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1.
转bar基因水稻恢复系的生理性状和农艺性状 总被引：5，自引：1，他引：5
通过杂交将转bar基因水稻亲本（Gulfmont、Bengal Hu-10和Cypress PB-6）中的bar基因转发转移到恢复系测64、明恢63和特青中，培育出6个抗除草剂（Liberty）的同型恢复系测64－B（G）、测64－B(C)、特青－B（C）、明恢63－B（G）和明恢63－B（C）。抗除草剂同型恢复系的大部分生理性状和主要农艺性状与原恢复系接近。将同型恢复系与不育系杂交，选配出8个抗除草剂三系杂交组合和2个抗除草剂两系杂交组合，抗除草剂组合能保持或略高于原组合的产量水平，利用这项技术有望解决杂交稻制种过程中出现的纯度问题。 相似文献
2.
GAO Fang-yuan REN Guang-jun LU Xian-jun HE Shu-lin CHEN Xiao-juan LU Dai-hua SUN Shu-xia LI Zhi-hua LIU Guang-chun ZHANG Yi-zheng 《水稻科学》2009,16(3):194-200
Bt5198, a new rice restorer line containing Bt gene, was developed from the cross and backcross of the elite restorer line Chenghui 177 with Bt Minghui 63, a transgenic Bt restorer line. The inbred lines were evaluated using PCR amplification, test paper evaluation, insect resistance evaluation in both the laboratory and paddy fields, nursery evaluation of rice blast resistance and pedigree selection of agronomic traits. Larval mortalities on Bt5198 and Bt Minghui 63 were 100% when rice culms were inoculated with the eggs of the striped stem borer (SSB) in the laboratory. Bt5198 was highly resistant against SSB and the yellow stem borer (YSB) under field conditions. The F1 hybrids derived from Bt5198 and four cytoplasmic male sterile (CMS) lines were also highly resistant to SSB and YSB and had a significant heterosis. Two-year evaluation of rice blast resistance confirmed that the resistance levels of Bt5198 to leaf blast and neck blast were similar to those of Chenghui 177 and significantly better than those of Bt Minghui 63. Seed germination ability and pollen yield of Bt5198 were similar with Chenghui 177, suggesting that the introduction of the Bt gene into the new restorer line had no significant effects on seed vitality or the yield of seed production. To identify the presence of the Bt gene, it was effective to combine test paper examination with the evaluation of insect-resistance, both in the laboratory and under field conditions. 相似文献
3.
利用分子标记辅助育种技术选育高抗白叶枯病恢复系 总被引：13，自引：2，他引：13
通过常规回交育种结合分子标记辅助选择技术,已将来自IRBB24的2个抗白叶枯病基因, Xa21和Xa4, 聚合到感病的杂交稻恢复系绵恢７２５中。通过分子标记检测目标基因和亲本遗传背景差异分析快速获得了4个高抗白叶枯病的恢复系姊妹系R207 1、R207 2、R207 3和R207 4。采用与目标抗性基因相应的菲律宾菌系P1、P6 和7个中国病原型代表菌系（CⅠ~CⅦ）对4个姊妹系及其杂交组合进行田间接种。结果表明,这些姊妹系及所配制的杂交组合抗病性强、抗谱广,其中杂交组合G46A/R207 2具有良好的产量潜力,将R207 2定名为蜀恢207。 相似文献
4.
DENG Qi-ming WANG Shi-quan ZHENG Ai-ping ZHANG Hong-yu LI Ping 《水稻科学》2006,13(1):22-28
Rice bacterial blight, one of the major diseases of rice caused by Xanthomonas oryzae pv. oryzae, Xoo, jeopardizes rice diversely. It causes leaf wilting, affects photosynthesis and reduces 1000-grain weight and generally results in yield loss by 20-30 pe… 相似文献
5.
转基因杂交稻组合汕优63和汕优559对白叶枯病的抗性 总被引：5，自引：1，他引：4
采用包括与Xa21相匹配的鉴别菌系在内的12个国际鉴别小种和中国7个病原型，接种由明恢63、盐恢559的Xa21转基因单拷贝抗性纯合系与珍汕97A杂交获得的转基因杂交稻汕优63 (汕优63-Xa21 73个 F1群体和转基因杂交稻汕优559（汕优559-Xa21 13个F1群体，测定转基因杂交稻对白叶枯病的抗性。结果表明这2个转基因杂交稻组合具有优良的广谱抗性， 抗谱与Xa21基因供体IRBB21相同，非转基因杂交稻表现感病。 相似文献
6.
香5是由湖北省农科院选育的优质两系杂交稻恢复系,所配组合广两优5号(广占63-4S/香5)于2013年通过了湖北省审定.利用回交和分子标记辅助选择技术,将供体亲本MD12086-1351中的抗稻瘟病基因Pi9、抗褐飞虱基因Bph14、Bph15和抗白叶枯病基因Xa23渗入到香5背景中,育成了3个同时携带Pi9、Bph14、Bph15和Xa23基因的新株系.鉴定结果表明,新株系的叶瘟抗性明显提高,穗颈瘟抗性部分提高,苗期抗褐飞虱,分蘖盛期高抗白叶枯病;产量、主要农艺性状、香味和稻米品质主要指标与香5相似.新株系所配的组合在产量、主要农艺性状上与香5所配的组合相似.表明新株系可以作为香5的替代系用于培育抗稻瘟病、抗褐飞虱和抗白叶枯病的两系杂交稻新组合. 相似文献
7.
转基因抗虫性水稻恢复系选育及特性分析 总被引：4，自引：1，他引：3
将优质抗稻瘟病水稻恢复系成恢177与转基因水稻Bt明恢63杂交并回交1次,采用PCR分析、试纸条检测、室内和田间人工接虫鉴定抗虫性,并结合系谱法选择农艺性状,病圃接种鉴定稻瘟病抗性,育成具转基因抗虫性的水稻新恢复系Bt5198。采用离体茎秆法接种二化螟卵块,新恢复系Bt5198和Bt明恢63的幼虫死亡率均为100%。在田间人工接虫条件下,该恢复系对二化螟和三化螟均表现高抗,与4个不育系配制的杂种F1仍保持良好的抗虫性,且杂种优势明显。两年稻瘟病抗性鉴定结果表明,新恢复系Bt5198的叶瘟和颈瘟抗性水平与成恢177相当,明显优于Bt明恢63。Bt5198的种子发芽率和花粉量与成恢177相当,Bt基因导入水稻恢复系不会对种子生活力和制种产量造成显著影响。在无选择标记基因的转基因后代中,结合利用试纸条检测和室内、田间人工接虫鉴定是筛选Bt基因抗虫性的有效方法。 相似文献
8.
Recent Advances in Development of Herbicide Resistant Transgenic Hybrid Rice in China 总被引：3，自引：0，他引：3
XIAO Guo-ying 《水稻科学》2009,16(3):235-239
In addition to weed control in direct seeding field of hybrid rice, herbicide resistance genes were used by Chinese scientists to increase and identify the purity of hybrid seeds, and to realize the mechanization of hybrid seed production. The elite restorer lines, such as Minghui 63, R752, T461, R402, D68 and E32 were transformed directly with herbicide resistance genes, in which D68 and E32 are restorer lines of two-line system and the others are of three-line system. Because almost all of important restorer lines are indica varieties and are recalcitrant in transformation, many herbicide resistant near-isogenic restorer lines were developed by sexual hybridization of indica and japonica varieties and backcross with indica restorer lines later, such as Ce 64, Minghui 63, Teqing, Milyang 46, R402 and 9311, in which 9311 is a restorer line of two-line system. The elite photoperiod-sensitive/thermo-sensitive genic male sterile lines, such as Pei'ai 64S, P88S, 4008S and 7001S, were transformed with herbicide resistance genes. A few herbicide resistant male sterile lines were developed through sexual hybridization and subsequently systemic selection, such as Bar1259S, Bar2172S, 05Z221A and 05Z227A. With the employment of herbicide resistant male sterile lines or herbicide resistant restorer lines, a few herbicide resistant hybrid rice combinations were developed, such as Xiang 125S/Bar 68-1 and Pei'ai 64S/Bar 9311. Based on herbicide resistance, the research was marching on to investigate the parental lines of hybrid rice with insect resistance, drought tolerance, etc. 相似文献
9.
分子标记辅助选择抗稻白叶枯病基因在选育水稻广亲和恢复系上的应用 总被引：18，自引：2，他引：18
以IRBB21为Xa21基因供体,广亲和恢复系4183为受体，进行1次杂交并回交3次，逐代用分子标记检测手段，导入广谱抗白叶枯病基因Xa21,在保持广亲和恢复系4183优良经济性状的基础上，增强其抗白叶枯病的能力，育成抗白叶枯病的广亲和恢复系抗4183，其抗性达到了IRBB21的抗性水平，且保持了4183的广亲和性、恢复性及优良的经济性状。并就杂交水稻白叶枯病的抗性改良及育种对策进行了讨论。 相似文献
10.
籼型恢复系绵恢2040的选育与应用 总被引：1，自引：0，他引：1
绵恢2040系西南科技大学水稻研究所用万恢228与多系I号杂交选育而成的具有粳稻亲缘的籼型恢复系，农艺、遗传性状稳定，具有恢复力和配合力强，较抗稻瘟病、品质较好、制种花粉量大、易获高产等特点；播抽期较明恢63短5d左右，温光反应特性与明恢63相似。所配组合B优840和C优2040已通过四川省农作物品种审定委员会审定。 相似文献
11.
D型杂交稻恢复系主效恢复基因的剖分与遗传效应分析 总被引：2，自引：0，他引：2
利用明恢63与D297A构建的近等基因系对D型杂交水稻恢复系主效恢复基因进行了剖分，连续回交7代后，经自交和测交筛选并结合农艺性状比较和SSR分子标记辅助选择，将明恢63的主效恢复基因剖分到具有D297A遗传背景的近等基因系818中，该近等基因系遗传背景与297A相似，SSR标记差异为1.5%。对近等基因系818恢复基因的遗传分析证明，该近等基因系具有一对主效恢复基因。近等基因系818与不同类型的不育系杂交后在不同生态地区的试验表明，近等基因系818所含单个主效恢复基因的恢复力与明恢63所含有的2对以上恢复基因的恢复力表现没有差异，并且在不同遗传背景和生态环境中的表现相似；单个主效恢复基因的恢复力在不同生态环境的表现稳定。 相似文献
12.
转溶菌酶基因水稻回交转育籼型杂交稻亲本 总被引：6，自引：0，他引：6
以抗稻瘟病的粳稻中花9号转溶菌酶基因材料D2 1 2与籼型杂交稻两优培九的恢复系9311及不育系培矮64S、汕优63的恢复系明恢63分别杂交和多代回交,进行外源溶菌酶基因的转育。对获得的转育回交后代B39311、B3MH63、B2PA64S和回交自交后代B2F29311、B2F2MH63、B1F2PA64S进行了PCR分析,表明外源基因在回交后代中呈1∶1分离,而在回交的自交后代中呈3∶1分离,证明外源溶菌酶基因是以单拷贝稳定传递给后代的。2003年和2004年对转育后代和测交组合进行了稻瘟病抗性调查,结果表明,转育后代抗性与轮回亲本相比、测交组合抗性与对应杂交稻组合相比都有了较大提高。随着转育回交世代的增加,抗性增强得越明显。研究表明通过回交转育方法将外源溶菌酶基因导入杂交稻亲本是选育抗稻瘟病杂交稻新组合的一条简便有效的途径。 相似文献
13.
A total of 638 isolates of rice blast(Magnaporthe oryzae) were isolated in 2002-2009 from different rice varieties in different regions of Sichuan,China and inoculated onto seven rice varieties(Lijiangxintuanheigu,IR24,Minghui 63,Duohui 1,Chenghui 448,Neihui 99-14 and RHR-1) to differentiate the virulence types of the fungus and trace the changes.The virulence to the seven varieties was respectively scored at 1,2,4,8,16,32 and 64.The total scores of individual M.grisea isolates which were the sum of scores infecting differential varieties could,in turn,be used for the nomenclature of the virulence types due to their accordance to the special virulence patterns.The 638 tested isolates were then differentiated into 56 different virulence types.Type 15 virulent to Lijiangxintuanheigu,IR24 and Minghui 63,and Type 127 virulent to all of the seven varieties were the most dominant virulence types respectively with the occurrence frequencies of 15.99% and 15.83%.Type 19 and other seven virulence types were not monitored during 2002-2009.Type 15 was the predominant virulence type in 2002,2003,2004 and 2007,whereas Type 127 had been the most dominant virulence type after 2005 except for the year 2007 when the province underwent severe drought.Five hundred and seven out of the 638 tested isolates were virulent to Minghui 63,and 89.58% of the 384 isolates virulent to either Duohui 1,Chenghui 448 or Neihui 99-14 were virulent to Minghui 63,which indicated the impact of the extensive plantation of hybrid rice Minghui 63 as the restorer line on the virulence evolution of M.oryzae in Sichuan.The virulence pattern of the dominant virulence types suggested that the acquiring of virulence to all the major resistant restorer lines was the main routes of the evolution in virulence of M.oryzae to hybrid rice in Sichuan.The virulence frequencies of the 638 tested isolates to IR24,Minghui 63,Duohui 1,Chenghui 448,Neihui 99-14 and RHR-1 were respectively 74.6%,79.5%,73.8%,37.0%,39.0% and 40.4%.The analysis for the sources of the different virulence type isolates indicated the pathogen on the newly released resistant varieties were stronger than conventional rice varieties which had become susceptible in the field since 1980s. 相似文献
14.
Heng-Xiu Yu Qiao-Quan Liu Li Xu Mei-Fang Lu Xiao-Jun Yang Zhi-Yun Gong Xiu-Lin Cai Yuan-San Zhang Chang-Quan Zhang Zong-Yang Wang Ming-Hong Gu 《Journal of Cereal Science》2009
In rice grains, high amylose content (AC) is correlated with poor grain quality, particularly in indica hybrid rice. To obtain indica hybrid rice with improved cooking and eating qualities, we introduced the antisense Waxy (Wx) gene into 2 elite parental lines of indica hybrid rice by using co-transformation methods. Subsequently, we selected several elite homozygous transgenic lines that did not contain the selectable marker. The expression of the endogenous Wx gene of the selected transgenic lines was significantly downregulated, resulting in low AC in the mature seeds; moreover, the AC in some lines reduced to the level observed in glutinous rice. With the decrease in AC, the gel consistency of the transgenic rice became softer, and the gelatinization temperature tended to be higher than those of the wild types, especially in the case of the Longtefu-derived transformants. We also analyzed the pasting properties of the selected transgenic low-AC lines, and we noted an improvement in the pasting properties of the transgenic rice lines. The results from a field trial indicated that the grain weights of the transgenic lines with lower AC exhibit remarkable reduction compared with those of the wild types. 相似文献
15.
IRBB近等基因系对白叶枯病混合菌系的抗性表现及在恢复系选育中的应用 总被引：1，自引：0，他引：1
利用浙江省流行小种混合菌系对IRBB近等基因系进行抗白叶枯病鉴定，结果表明，多个白叶枯病抗性基因的聚合有利于抗性的提高。育种上可利用聚合有多个白叶枯病抗性基因的IRBB52、IRBB54、IRBB60等作为抗源供体，与抗稻瘟病恢复系CDR448、明恢63等杂交，利用花培、常规遗传选育等方法，选育双抗恢复系。 相似文献
16.
四川主要水稻恢复系抗白叶枯病基因的分子检测 总被引：2，自引：0，他引：2
用分别与水稻抗白叶枯病基因Xa4、Xa7、Xa21和Xa23紧密连锁的PCR标记MP12、M3、pTA248和RM206对四川省新选育的以及主要应用的39份恢复系进行了分子检测，结果表明，这些恢复系均不含有Xa7（M3）和Xa21（pTA248）抗性等位点，17份携有榭（MP12）抗性等位点，少数（6个）携有Xa23（RM206）抗性等位点。在杂交水稻恢复系抗性改良中，应采用分子标记辅助选择（MAS）加快引入利用除工卅外的其它儿个显性抗性基因。 相似文献
17.
水稻三系恢复系镇恢084的螟虫抗性改良 总被引：1，自引：0，他引：1
以对螟虫有良好抗性的T1C - 19(含抗螟虫的cry1C*基因)作为供体,优良恢复系镇恢084为受体和轮回亲本,通过杂交、回交并结合分子辅助选择的方法将cry1C*基因导入镇恢084中,选育了携带cry1C*基因的纯合株系4个.对育成的抗螟虫新株系及其杂交组合的CRY1C*蛋白表达量、螟虫抗性、产量、主要农艺性状和稻米品质进行了研究.结果表明,4个抗虫新株系的CRY1C*蛋白表达量与供体T1C - 19基本一致,表明cry1C*基因在新株系中能够很好地表达;在自然条件下的田间调查结果显示,培育的4个抗螟虫株系及其所配杂交组合与供体亲本表现一致,都没有受到螟虫的危害,而受体亲本受到一定程度的螟虫危害.此外,新育成的4个抗螟虫株系在主要农艺性状、稻米品质和配合力上都与受体亲本镇恢084相近. 相似文献
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19.
R. Abdul Fiyaz D. Shivani K. Chaithanya K. Mounika M. Chiranjeevi G. S. Laha B. C. Viraktamath L. V. Subba Rao R. M. Sundaram 《水稻科学》2022,29(2):118-132
The production and productivity of rice has been challenged due to biotic and abiotic factors. Bacterial blight (BB) disease, caused by Xanthomonas oryzae pv. oryzae, is one of the important biotic stress factors, which reduces rice production by 20%–50%. The deployment of host plant resistance is the most preferred strategy for management of BB disease, and breeding disease resistant varieties remains a very economical and effective option. However, it is difficult to develop rice varieties with durable broad-spectrum resistance against BB using conventional approaches alone. Modern biotechnological tools, particularly the deployment of molecular markers, have facilitated the cloning, characterization and introgression of BB resistance genes into elite varieties. At least 46 BB resistance genes have been identified and mapped from diverse sources till date. Among these, 11 genes have been cloned and characterized. Marker-assisted breeding remains the most efficient approach to improve BB resistance by introducing two or more resistance genes into target varieties. Among the identified genes, xa5, xa13 and Xa21 are being widely used in marker-assisted breeding and more than 70 rice varieties or hybrid rice parental lines have been improved for their BB resistance alone or in combination with genes/QTLs conferring tolerance to other stress. We review the developments related to identification and utilization of various resistance genes to develop BB resistant rice varieties through marker-assisted breeding. 相似文献
20.
YI Zi-li WANG Zi-xuan QIN Jing-ping JIANG Jian-xiong TAN Yan-ning ZHOU Qing-ming 《水稻科学》2006,13(4):234-242
Rice blast (Pyricularia oryzae Cavara), one of the major fungal diseases in rice fields all over the world, is induced by the ascomycetous fungus Magnaporthe grisea (Hebert) Barr [Anamorph: Pyricularia grisea (Cooke) Sacc.] [1]. According to the statistic… 相似文献