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1.
Wheat pre-breeding using wild progenitors 总被引：6，自引：1，他引：6
J. J. Valkoun 《Euphytica》2001,119(1-2):17-23
To facilitate the use of wheat wild relatives in conventional breedingprograms, a wheat pre-breeding activity started at ICARDA in 1994/1995season. Preliminary results of gene introgression from wild diploidprogenitors, Triticum urartu, T. baeoticum, Aegilops speltoides andAe. tauschii and tetraploid T. dicoccoides are described. Crosseswith wild diploid Triticum spp. yielded high variation in plant andspike morphology. Synthetic hexaploids were produced from crosses of alocal durum wheat landrace `Haurani' with two Ae. tauschiiaccessions. Both Ae. tauschii accessions carry hybrid necrosis allelesthat gave necrotic plant phenotypes in crosses with some bread wheats.Backcross progenies with agronomical desirable traits, i.e. high spikeproductivity, short plant stature, earliness, drought tolerance and highproductive tillering, were identified in crosses of durum wheat with wild Triticum spp. and in a cross of one of the hexaploid synthetics with alocally adapted bread wheat cv. `Cham 6'. Resistance to yellow rust wasfound in durum wheat crosses with the three wild Triticum spp. andAe. speltoides and leaf rust resistance was identified in crosses withT. baeoticum and Ae. speltoides. The results show that wheatimmediate progenitors may be a valuable and readily accessible source ofnew genetic diversity for wheat improvement. 相似文献
2.
Noel E.A. Murphy Robert Loughman Robin Wilson Evans S. Lagudah Rudi Appels Michael G.K. Jones 《Euphytica》2000,113(3):227-231
Septoria nodorum blotch is the most important leaf disease of wheat in Western Australia. A potentially useful source of resistance
has been identified in an accession of Aegilops tauschii. To study the genetics of resistance of this source a cross was made between the resistant Ae. tauschii accession, RL5271, and a susceptible accession, CPI110889. The resistant parent took significantly longer to develop symptoms,
developed significantly fewer lesions and expressed significantly lower levels of disease than the susceptible parent. The
F1 mean response for disease severity indicated there was no complete dominance. The F3 families were classified using three
approaches. In the first approach the individual F3 plant response was used to classify the F3 families. In the second approach
the F3 family means and standard errors were used to classify the F3 families. In the final approach Best Linear Unbiased
Predictors of disease score and standard error for each F3 family derived from a REML analysis were used to classify the F3
families. The genotypic ratios generated by each of the approaches suggested that resistance is controlled by a single gene.
The effectiveness of the resistance and its simple genetic control in the Ae. tauschii, accession RL5271 may be a useful resistance source for use in a bread wheat breeding program.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
3.
Diploid wheat, Triticum monococcum s.l., is a host for the wheat leaf rust fungus, Puccinia triticina. Some accessions have been reported to show a high degree of prehaustorial resistance. This is non-hypersensitivity resistance,
which acts before the formation of haustoria by the pathogen. To assess the frequency of prehaustorial resistance 598 accessions
of diploid wheat were inoculated with the wheat leaf rust isolate Felix. Most T. monococcum s.s. accessions (84%) were resistant whereas all T. urartu and all but three T. boeoticumaccessions were susceptible. Histological components analysis revealed that a high percentage of prehaustorial resistance
to P. triticina was found in only three T. monococcum accessions. No haustoria were observed in such infection units confirming the prehaustorial nature of the resistance. Prehaustorial
abortion of certain infection units in an accession always coincided with posthaustorial abortion of the other infection units.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
4.
Summary Resistance to septoria nodorum blotch in Triticum monococcum, T. tauschii, T. timopheevii, T. dicoccum and T. durum was evaluated on plants at the three-leaf stage in greenhouse tests. A high frequency of resistant genotypes was found in T. monococcum, T. tauschii and T. timopheevii, but not in T. dicoccum and T. durum. The resistance of F1 plants of crosses of resistant T. monococcum (PI 289599) and T. timopheevii (PI 290518) accessions with susceptible common wheat cv. Park and durum wheat cv. Wakooma, respectively, was evaluated on the basis of percentage leaf necrosis, lesion number, lesion size and incubation period. No dominance was found for long incubation period, but various dominance relationships occurred for low percentage leaf necrosis, low lesion number and small lesion size, depending on the cross. Multiple regression analysis showed that lesion number contributed more to percentage leaf necrosis than lesion size or incubation period. Resistance to septoria nodorum blotch was transferred successfully from T. timopheevii to cultivated durum wheat. Resistant BC1F7 lines, recovered from the T. timopheevii (PI 290518) × Wakooma cross, showed normal chromosome behaviour at meiosis (14 bivalents) and were self-fertile. However, an effective level of resistance was not recovered in lines derived from the other interspecific crosses. 相似文献
5.
Variability of high molecular weight glutenin subunits (HMW-GS) was studied in198 accessions of Ae. Tauschii (2n=2x=14, DD) by sodium dodecyl sulphate(SDS-PAGE) and acid polyacrylamide gel electrophoresis (A-PAGE) and capillary electrophoresis
(CE). A high allelic variation of HMW-GS, including some novel x- and y-type subunits and variable subunit combinations were
observed. One accession(TD159) showed a x-type null form. The results by A-PAGE analysis revealed that the subunits Dx5
t and Dy10
t encoded by Glu-D
t
1 locus in Ae. tauschii were different in relative mobilities in comparison with the subunits Dx5 and Dy10 found in bread wheats, whereas they had
the same mobilities, respectively, when separated by SDS-PAGE. The higher resolution of Ae. tauschii HMW-GS separated by CE method showed two clear peaks in accordance with x- and y-type subunits, respectively,except the accession
TD151 which possessed only subunit Dy12.1*t. The electro elution time of the x-type and y-type subunits were about 13–14 and 7–8minutes, respectively. Characterization
of wheat HMW-GS was facilitated by using CE which provides high resolution and increases the speed of analysis in conjunction
with the traditional gel electrophoretic methods. A total of 42HMW-GS alleles were identified, among which were several alleles
not presently detected in bread wheats. Hence Ae. tauschii is potentially a valuable genetic resource for quality improvement of bread wheat.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
6.
Langdon durum D-genome disomic substitution lines were used to study the chromosome locations of adult-plant leaf rust resistance genes identified from tetraploid wheat accessions. The accessions are 104 (Triticum turgidum subsp. dicoccum var. arras) and 127 (T. turgidum subsp. durum var. aestivum). The complete sets of the substitution lines were crossed as female parents with the accessions and F1 double monosomic individuals selected at metaphase I. Segregating F2 individuals were inoculated during the flag leaf stage with pathotype UVPrt2 of Puccinia triticina. The substitution analysis involving accession 104 showed that the gene for leaf rust resistance is located on chromosome 6B. The analysis with accession 127 indicated that chromosome 4A carries a gene for leaf rust resistance. The two novel genes are temporarily designated as Lrac104 and Lrac127, respectively from accessions 104 and 127. 相似文献
7.
Hydroxamic acid content of triticum species 总被引：2，自引：0，他引：2
Hermann M. Niemeyer 《Euphytica》1988,37(3):289-293
Summary Fifty-five accessions of Triticum species were analyzed for content of hydroxamic acids (Hx), a natural resistance factor against various organisms. Hx were found in all accessions analyzed. Extreme values were found in wild diploid species: highest in T. speltoides (16.0 mmol/kg fr. wt) and lowest in T. tauschii (0.21). Modern polyploid wheats sharing the same genome did not show substantial variations in Hx levels. The data suggest possible sources of high Hx levels for wheat breeding programs. 相似文献
8.
Triticum tauschii (Coss.) Schmal. is an ancestor of bread wheat (T. aestivum). This species has been widely used as a source ofsimply-inherited traits, but there are few reports of yield increases due tointrogression of genes from this species. Selections from F2-derivedlines of backcross derivatives of synthetic hexaploid wheats (T.turgidum / T. tauschii) were evaluated for grain yield in diverseenvironments in southern Australia. Re-selections were made in theF6 generation and evaluated for grain yield, yield componentsincluding grain weight, and grain growth characters in diverse environmentsin southern Australia and north-western Mexico. Re-selection was effectivein identifying lines which were higher yielding than the recurrent parent,except in full-irrigation environments. Grain yields of the selectedderivatives were highest relative to the recurrent parent in thelowest-yielding environments, which experienced terminal moisture deficitand heat stress during grain filling. The yield advantage of the derivativesin these environments was not due to a change in anthesis date orgrain-filling duration, but was manifest as increased rates of grain-filling andlarger grains, indicating that T. tauschii has outstanding potential forimproving wheat for low-yielding, drought-stressed environments. 相似文献
9.
10.
Summary Faster canopy development in temperate cereals can result in more efficient utilization of wate, light and nutrients if these are in limited supply. It can also result in more competitive plants and less use of herbicides. The possible importance of the coleoptile tiller for increasing early vigour in wheat (Triticum aestivum L.) was determined for twelve F4 lines in two experiments. Each F4 line was chosen for variation in the presence and absence of a coleoptile tiller in plants in the previous generation; each F4 line was traced to a single F2 plant from a multiple convergent cross of sixteen parents. Eight plants of each of the F4 lines were grown in a single row in both experiments. Just after the coleoptile tiller appeared, plants within each line were classified into one of two groups; one group contained plants with large coleoptile tillers whereas the other group had either small coleoptile tillers or none at all. Leaf expansion rates were determined non-destructively at regular intervals, and plants were harvested to determine leaf and tiller characteristics when there were about five mainstem leaves. There was substantial variation in the leaf area of the coleoptile tiller both between groups and among lines. In both experiments plants with a large coleoptile tiller had 24–30% more leaf area and dry weight than plants selected with a small or absent coleoptile tiller up to the final harvest. Leaf expansion rates (increase in leaf area per day) were 25 to 35% greater in the large coleoptile tiller selections from the time tillers first appeared to the time of harvest, whereas the relative leaf expansion rate (increase in leaf area per unit of leaf area present per day) of the large selections was significantly greater than the small selections only at the time of appearance of the coleoptile tiller and just after. There was no evidence that the early appearance of a coleoptile tiller was associated with the largest seedlings or that it inhibited the growth of later tillers. It is concluded that selection for the appearance of a large coleoptile tiller should improve the early vigour of temperate cereals.Abbreviations DAS
days after sowing
- LA
leaf area
- LAR
leaf area ratio
- LER
leaf expansion rate
- RLER
relative leaf expansion rate
- SLA
specific leaf area
- VPD
vapor pressure deficit
- +COL
large coleoptile tiller group
- –COL
small or absent coleoptile tiller group 相似文献
11.
Gert H. J. Kema 《Euphytica》1992,63(3):207-217
Summary Seven spelt wheat accessions of different origin were hybridized with the susceptible bread wheat cultivar Taichung 29 in order to study the genetics of their resistance to yellow rust (Puccinia striiformis Westend. f. sp. tritici). One Iranian and five European accessions were found to carry Yr5 of Triticum aestivum ssp. spelta var. album, whereas a factor for resistance in the Iranian accession 415 was confirmed to be genetically distinct from Yr5. The alleles for resistance in each of the accessions studied showed a monogenic dominant mode of inheritance. Twenty-eight spelt wheat accessions, including those studied for their resistance to yellow rust, were subjected to polyacrylamide-gel-electrophoresis to study variation for gliadin storage protein patterns. Thirteen distinct patterns were revealed, implying the presence of duplicates within the studied spelt wheat collection. 相似文献
12.
Quahir Sohail Tariq Shehzad Andrezj Kilian Amin Elsadig Eltayeb Hiroyuki Tanaka Hisashi Tsujimoto 《Breeding Science》2012,62(1):38-45
Aegilops tauschii Coss. is the D-genome donor to hexaploid bread wheat (Triticum aestivum) and is the most promising wild species as a genetic resource for wheat breeding. To study the population structure and diversity of 81 Ae. tauschii accessions collected from various regions of its geographical distribution, the genomic representation of these lines were used to develop a diversity array technology (DArT) marker array. This Ae. tauschii array and a previously developed DArT wheat array were used to scan the genomes of the 81 accessions. Out of 7500 markers (5500 wheat and 2000 Ae. tauschii), 4449 were polymorphic (3776 wheat and 673 Ae. tauschii). Phylogenetic and population structure studies revealed that the accessions could be divided into three groups. The two Ae. tauschii subspecies could also be separately clustered, suggesting that the current taxonomy might be valid. DArT markers are effective to detect very small polymorphisms. The information obtained about Ae. tauschii in the current study could be useful for wheat breeding. In addition, the new DArT array from this Ae. tauschii population is expected to be an effective tool for hexaploid wheat studies. 相似文献
13.
R. P. de San Celedonio L. G. Abeledo J. M. Brihet D. J. Miralles 《Journal of Agronomy and Crop Science》2016,202(5):409-420
The aim of this study was to analyse (i) the crop attributes that determine flowering time (i.e. final leaf number, FLN; and phyllochron, Phy), (ii) the dynamics of tiller appearance and (iii) the synchrony between leaf and tiller appearance in wheat and barley plants exposed to waterlogging. Two experiments were carried out in pots, in which wheat and barley cultivars were exposed to five waterlogging treatments, during different periods throughout the crop cycle, from emergence to maturity. The appearance of leaves and tillers on the main stem was measured twice a week in labelled plants. Waterlogging from emergence to flag leaf appearance significantly delayed time to flowering. The delay was greater when waterlogging occurred at the beginning of tillering, lengthening the period from emergence to flowering 24 % (13–15 days) in barley and 10–15 % (6–10 days) in wheat, as compared to control. Phy was the main attribute explaining the delay in flowering, as FLN was not altered. Waterlogging during the early stages of development reduced tiller appearance rate (TAR) in both species, but this effect was partially counterbalanced by a lengthening of the tillering phase, so the effect on final tiller number at maturity was limited. In conclusion, the exposure of wheat and barley to waterlogging during early stages of development delayed time to flowering and reduce TAR in both species. Waterlogging during more advanced crop stages produced slight effects on tillering dynamics, which would indicate that waterlogging affected structure generation more than mortality. 相似文献
14.
C. M. Konik-Rose S. Rahman R. Appels R. Moss G. McMaster D. R. Marshall F. L. Stoddard 《Euphytica》2009,167(2):203-216
Greater variability in starch properties is found in lower ploidy wheats than in commercial hexaploid wheats. This paper reports
on the starch properties and variability in granule bound starch synthase (GBSS) loci of 17 diploid (Aegilops tauschii) and 12 tetraploid (durums) potential progenitors of wheat, compared with 29 synthetic hexaploid wheats produced from such
progenitors. Starch properties examined were granule size distribution, swelling power, amylose content, gelatinisation and
amylose-lipid dissociation properties. A PCR screening method was able to detect the presence or absence of each of the three
GBSS genes. It also detected polymorphisms in eight diploids and nine hexaploids, all displaying the same 25 bases deletion
in the D genome allele of GBSS. Two tetraploids and five hexaploids were null 4A for GBSS. There was little difference in
the amylose contents and amylose-lipid dissociation peak temperatures of the synthetic hexaploids and the lower ploidy wheats.
The synthetic hexaploids showed intermediate swelling power values with the durums giving the highest swelling powers. The
durums also had higher B granule contents than the A. tauschii accessions, but not as high as the synthetics. However, the A. tauschii samples gave the highest gelatinisation peak temperatures. The presence of the null 4A mutation was positively correlated
with swelling power, amylose content and DSC measurements. The new smaller D genome allele of GBSS was associated with slightly
higher swelling power. These results confirm the value of wheat progenitor lines as sources of new starch properties for hexaploid
wheat.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
15.
Summary The genus Paspalum contains a number of important forage grasses, including P. dilatatum Poir. (dallisgrass) and P. notatum Flugge (bahiagrass). Even though many Paspalum species are adapted to moist, humid areas, they frequently are subjected to extended periods of drought stress which reduces forage productivity. This study was initiated to determine the range of variation for different indicators of drought tolerance in several Paspalum species. Fifteen species representing a broad cross section of germplasm were grown in a replicated field nursery and sampled for leaf epicuticular wax content on four dates over three years. Wax was extracted with chloroform and quantified by the dichromate oxidation technique. Heat tolerance (solute leakage technique) was also determined by calculating the ratio of the conductivity of the external bathing solution of leaves following a heat treatment to the conductivity of the bathing solution after leaves were boiled. Wax values of the entries ranged from 5.7 to 0.88 mg/dm2. Both relative rankings and absolute wax values were very consistent across all sampling dates. The accessions of Paspalum nicorae Parodi and P. quandrifarum Lam. were consistently high in wax content, while the accessions of P. commune Lillo and P. unispicatum (Scribn. et Merr.) Nash were consistently low. The heat tolerance testing demonstrated that the accession of P. notatum var. saurae Parodi was the most heat tolerant of the entries. Results from this investigation demonstrate the wide range of diversity that exists for both epicuticular wax and heat tolerance within the genus Paspalum. 相似文献
16.
The greenbug, Schizaphis graminum(Rondani), the Russian wheat aphid, Diuraphis noxia (Mordvilko), and the bird cherry oat aphid, Rhopalosiphum padi(L.), annually cause several million dollars worth of wheat production losses in Europe and the United States. In this study,
Triticum and Aegilops accessions from the Czech Research Institute of Crop Production and the Kansas State University Wheat Genetic Resources Center
were evaluated for resistance to these aphids. Accessions with aphid cross-resistance were examined for expression of the
antibiosis, antixenosis, and tolerance categories of resistance. Aegilops neglecta accession 8052 exhibited antibiotic effects toward all three aphids in the form of reduced intrinsic rate of increase (rm). The rm of greenbug (biotype I) on Ae. neglecta 8052 was significantly lower than that of greenbugs on plants of the susceptible U. S. variety Thunder bird. The rm of Russian wheat aphids was significantly lower on foliage of both Ae. neglecta 8052 and T. araraticum accession 168 compared to Thunderbird. The rm values of bird cherry oat aphids fed both Ae. neglecta 8052 and T. araraticum 168 were also significantly lower than those fed the susceptible accession T. dicoccoides 62. Neither Ae. neglecta 8052 or T. araraticum 168 exhibited tolerance to either greenbug biotype I or Russian wheat aphid. Preliminary data suggest that T. araraticum 168 may also possess tolerance to bird cherry oat aphid. New genes from Ae. neglecta 8052 and T. araraticum 168 expressing aphid antibiosis can be used to develop multiple aphid resistant wheat in the U. S. and Central Europe.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
17.
Summary Six wild barley (Hordeum spontaneum) accessions, from a diverse range of habitats, and two spring-cultivated barleys, were examined for variation in durations of development phases. The durations of the leaf initiation and spikelet initiation phases were longer and spikelet growth phases shorter, in wild than in cultivated barley. Across all wild and cultivated barleys the rate and duration of spikelet initiation were negatively correlated, but neither was related to the number of spikelets per spike. The number of spikelets was positively correlated with the number of leaves and the ratio of the number of spikelets to the number of leaves declined with increasing time to anthesis, indicating that each successive leaf was associated with a diminishing increase in the number of spikelets. The duration of culm elongation and final culm length were shorter in accessions of cultivated barley compared with wild barley. This paper also discusses the feasibility for increasing the number of spikelets per spike through breeding for genetic changes in lengths of pre-anthesis phases of development.Abbreviations ANOVA
Analysis of variance
- HV
Hordeum vulgare
- CE
Culm elongation
- DR
Double ridge
- HS
Hordeum spontaneu
- ° Cd
Degree days 相似文献
18.
Resistance to stripe rust (caused by Puccinia striiformis Westend.) of 34 Triticum turgidum L. var.durum, 278 T. tauschii, and 267 synthetic hexaploid wheats (T. turgidum x T. tauschii) was evaluated at the seedling stage in the greenhouse and at the adult-plant stage at two field locations. Mexican pathotype 14E14 was used in all studies. Seedling resistance, expressed as low infection type, was present in all three species. One hundred and twenty-eight (46%) accessions of T. tauschii, 8 (23%) of T. turgidum and 31 (12%) of synthetic hexaploid wheats were highly resistant as seedlings. In the field tests, resistance was evaluated by estimating area under disease progress curve (AUDPC). Synthetic hexaploid wheats showed a wide range of variability for disease responses in both greenhouse and field tests, indicating the presence of a number of genes for resistance. In general, genotypes with seedling resistance were also found to be resistant as adult plants. Genotypes, which were susceptible or intermediate as seedlings but resistant as adult plants, were present in both T. turgidum and the synthetic hexaploids. Resistances from either T. turgidum or T. tauschii or both were identified in the synthetic hexaploids in this study. These new sources of resistance could be incorporated into cultivated hexaploid wheats to increase the existing gene pool of resistance to stripe rust. 相似文献
19.
Glaucous leaf and tough rachis phenotypes are rare in Aegilops tauschii, the D genome donor to common wheat (Triticum aestivum). The genes for glaucous leaf and tough rachis were mapped using microsatellite probes in A. tauschii. The glaucous phenotype was suppressed by the inhibitor W2I located on chromosome 2DS. The gene W2I was mapped to the distal part of 2DS, and was unlinked to the centromere. This suggests that the distance of the W2I locus from the centromere was maintained during the evolution of hexaploid wheat from its diploid progenitors as the inhibitor
gene is at the same position in A. tauschii and bread wheat. The Brt (Brittle rachis of A. tauschii) locus was located on the short arm of chromosome 3D, and was 19.7 cM from the centromeric marker, Xgdm72.3D. Brt causes breakage of the spike at the nodes, thus creating barrel-shaped spikelets, while Br1 in hexaploid wheat causes breakage above the junction of the rachilla with the rachis such that a fragment of rachis is attached
below each spikelet. 相似文献
20.
It was recently shown that allopolyploidy brings novel epistatic interactions to genes belonging to different genomes. However, systematic studies of the phenotypic relationships between synthetic hexaploid wheats and their parental lines have not been conducted. In this study, 27 synthetic hexaploid wheats were produced by crossing the tetraploid wheat cultivar ‘Langdon’ with 27 accessions of Aegilops tauschii. Variations in 20 morphological and flowering traits were analysed in both the synthetic wheat lines and the parental Ae. tauschii accessions. The 20 traits exhibited large variations in the wheat lines. For many of the traits, the degree of variation in the parental accessions was reduced in the hexaploid derivatives. Principal component analysis of floret‐related traits divided the Ae. tauschii accessions into two subspecies, ssp. tauschii and ssp. strangulata, but this parental pattern of subspecific division was not detectable in the hexaploids. Our results suggest that the ‘Langdon’ genome may have an alleviating effect on the expression of D‐genome‐derived variations in derived synthetics. 相似文献