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1.
The aim of the present work was to determine the influence of different CaCl(2) dosages on foliar biomass and quality of tobacco plants (Nicotiana tabacum L. var. Tennesse 86). Plants were grown under controlled conditions and submitted to regular fertilization with macro- and micronutrients. The CaCl(2) was applied to the nutrient solution at 1.25 mM (T1), 2.5 mM (T2), and 5 mM (T3). The results indicated that, under the experimental conditions of this work, the application of 1.25 mM of CaCl(2) favored the growth and development of the leaves, this leading to improved biomass production in tobacco leaves. The increase in foliar biomass in treatment T1 could largely be a result of the stimulation of NO(3)(-) assimilation. However, this situation has negative consequences for tobacco quality, given that the T1 treatment augmented the NO(3)(-) concentration and the foliar concentration of nicotine (both effects being harmful for human consumption) and decreased the concentration of reducing sugars in leaves of tobacco plants compared with those of T2 and T3. Finally, a close and directly proportional relationship was found in our experiment between the parameters of foliar-biomass production, total N concentration, chlorophyll concentration, and decline in quality.  相似文献   

2.
叶面喷施尿素对葡萄氮代谢相关基因表达的影响   总被引：3，自引：1，他引：2  
本研究利用VitisEST数据库中EST序列片段结合RT-PCR方法克隆了与氮代谢相关的硝酸盐还原酶（NR）,亚硝酸还原酶（NiR）,谷氨酰胺合成酶（GS）,谷氨酸脱氢酶（GDH）和天冬酰胺合成酶（AS）的基因,并对它们进行亚细胞定位分析。同时,采用半定量RT-PCR和荧光定量PCR法研究葡萄叶面喷施不同浓度尿素后5个基因的表达变化。结果表明,5个基因在葡萄幼叶中的表达水平显著高于老叶,说明叶面喷施尿素以喷布到幼叶对5个基因的表达水平影响显著;5个基因在不同时间段的表达水平差异不一致,但表达水平均高于对照,以0.3%和0.5%浓度的尿素对其影响明显;适当提高尿素水平既能提高氮代谢基因的表达水平,又能提高果实大小等果实指标,使其达到同步增加。喷施尿素6 h后,GS的表达量上升幅度明显高于其它基因,而NR在喷施尿素后48 h内一直保持着比较高的表达水平;NiR、AS表达量的变化趋势分别与NR、GS相一致,并且NR﹥NiR,GS﹥AS。  相似文献   

3.
Zn, Se, and Fe levels in 65 Chinese rice samples were investigated, and the results indicated that these micronutrients contents of rice products from different location varied considerably. The mean contents of Zn, Se and Fe in these rice samples were 21.5+/-1.8, 0.020+/-0.012, and 12.4+/-4.3 mg kg(-1), respectively, which were too low to meet the micronutrient demands for the population feeding on the rice as staple. A field orthogonal experiment L9 (3(4)) was conducted on rice cultivar Wuyunjing 7, to evaluate the effect of Zn, Se, and Fe foliar fertilization on the concentration of these micronutrients, yield, and protein and ash content of rice grain. The results indicated that Zn and Se were the main variables influencing the Zn, Se, and Fe content of rice, and the optimal combination of fertilization for enhancing these micronutrients was 0.90 kg ha(-1) Zn, 0.015 kg ha(-1) Se, and 0.90 kg ha(-1) Fe. Under the optimal application condition, Zn, Se, and Fe content of rice could be significantly increased by 36.7%, 194.1%, and 37.1%, respectively, compared with the control, without affecting grain yield and protein and ash content of rice products. Moreover, in the confirmation experiment on rice cultivar Ninggeng 1, the optimal fertilization could increase the Zn, Se, and Fe content of rice up to 17.4, 0.123, and 14.2 mg kg(-1), respectively.  相似文献   

4.
Raman spectra of amino acids showed complexity in the C-H stretching region (2800-3100 cm(-)(1)) attributed to diversity of CH, CH(2), and CH(3) groups in the side chains, ionization state, and microenvironment. The involvement of specific amino acids in the C-H stretching region of selected proteins, namely, lysozyme, alpha-lactalbumin, beta-lactoglobulin, and their binary mixtures, was investigated by deconvolution using maximum likelihood techniques. The main protein band near 2940 cm(-)(1) was attributed not only to aromatic and aliphatic amino acids but also to many other amino acids. A band near 3065 cm(-)(1) was assigned to aromatic residues, whereas bands near 2880 and 2900 cm(-)(1) corresponded primarily to aliphatic amino acids. Heating at 90 degrees C increased relative intensity near 2940 cm(-)(1) and decreased relative intensity at 2895-2902 cm(-)(1) for lysozyme and its mixtures with alpha-lactalbumin or beta-lactoglobulin. Additional bands at 2812 or 2838 and 3003 cm(-)(1) were observed after heating or in 8 M deuterated urea, reflecting changes upon denaturation.  相似文献   

5.
《Journal of plant nutrition》2013,36(6):1089-1101
Abstract

Indian mustard (Brassica juncea L. cv. RH-30) was grown under different types and levels of nitrogen (N) sources, i.e. nitrate, ammonical, and nitrate plus ammonical, at 40, 80, and 120 kg ha? 1 under green house conditions. The plants were salinized with 8 and 12 dSm? 1 at 35 and 55 days after sowing. A progressive inhibition of the activity of enzymes of N metabolism, i.e., nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate synthase (GOGAT), and glutamate dehydrogenase (GDH), was observed with increasing level of salinity. However, the magnitude of such reductions was lowest at the highest level of N (120 kg ha? 1) as compared with the lowest level (40 kg ha? 1) irrespective of N source. The activity of nitrate-assimilating enzymes (NR and NiR) was maximum with nitrate fertilizer, and minimum with the ammonical form. The alleviation of detrimental effects of salinity on NR and NiR was better with the highest level of N (120 kg ha? 1) in nitrate form as compared with the lowest level of N (40 kg ha? 1). In contrast, the maximum activity of ammonium-assimilating enzymes (GS, GOGAT, and GDH) was observed with the highest level of N (120 kg ha? 1) and the minimum with the nitrate form of N under salinity. These results indicate that despite the high salinity, an increase in the concentration and uptake of N stimulates the activities of nitrate-assimilating enzymes (NR and NiR) as well as of the ammonia-assimilating enzymes (GS, GOGAT, and GDH).  相似文献   

6.
This study aimed to investigate the response of vegetative growth, yield, and some metabolic constituents of maize grains cv. Single Cross 124 to foliar applications of salicylic acid (SA; 100, 200, and 400 mg L?1) and thiourea (TU; 500, 1000, and 1500 mgL?1), two bioregulators, either alone or in combination. The foliar application of SA and TU alone significantly increased stem diameter, number of leaves?/?plant, leaf area, total dry weight?/?plant, leaf area index, net assimilation rate, specific leaf weight, and yield (i.e., ear length, ear diameter, number of grains?/?row, number of rows?/?ear, 100-grain weight, grain yield?/?plant, grain yield?/?fed (1 feddan = 4200 m2), harvest index, and shelling percentage) by increasing SA or TU concentrations up to 200 and 1500 mg L?1, respectively. Salicylic acid and TU, when applied alone, significantly improved the nutritional value and quality of maize grains by increasing crude protein, total soluble sugars, total free amino acids, and total soluble phenols.  相似文献   

7.
Three triterpenic acids, oleanolic acid, ursolic acid and maslinic acid, at 2 or 4 μmol/L were used to study their antiangiogenic potential in human liver cancer Hep3B, Huh7 and HA22T cell lines. The effects of these compounds upon the level and/or expression of hypoxia-inducible factor (HIF)-1α, basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), interleukin (IL)-8, urokinase plasminogen activator (uPA), reactive oxygen species (ROS), nitric oxide (NO) and cell invasion and migration were examined. Results showed that these triterpenic acids at 4 μmol/L significantly suppressed HIF-1α expression in three cell lines (P < 0.05); and these compounds at test doses failed to affect bFGF expression (P > 0.05). Three triterpenic acids dose-dependently decreased production and expression of VEGF and IL-8, retained glutathione level, lowered ROS and NO levels, and declined cell invasion and migration in test cell lines (P < 0.05). These compounds also dose-dependently reduced uPA production and expression in Hep3B and Huh7 cell lines (P < 0.05); but these agents only at 4 μmol/L significantly suppressed uPA production and expression in HA22T cells (P < 0.05). These findings suggest that these triterpenic acids are potent antiangiogenic agents to retard invasion and migration in liver cancer cells.  相似文献   

8.
【目的】 作物可以直接吸收小分子有机氮,探究不同氨基酸对作物产量、品质及养分吸收的影响,及其与锌肥配合的效果可深化对有机营养的认识,为叶面施肥提供理论依据。【方法】 以小白菜为供试作物进行盆栽试验,以喷施清水为对照,设甘氨酸、谷氨酸、苏氨酸、七水合硫酸锌分别单独喷施和甘氨酸、谷氨酸、苏氨酸分别与七水合硫酸锌混合喷施处理,三种氨基酸浓度梯度均为100、250、400 mg/L,七水合硫酸锌施用浓度为0.1%,共20个处理;每个处理5次重复,随机区组排列;收获后测定植株生物量和硝酸盐、可溶性糖及维生素C含量,分析植株吸收锌量。【结果】 1）施用三种氨基酸均能明显促进小白菜生长、改善品质,并能显著促进小白菜对锌的吸收。与对照相比,喷施三种氨基酸小白菜生物量分别提高了32.0%、19.8%和16.6%,硝酸盐含量分别降低了53.9%、60.9%和65.6%,可溶性糖含量分别提高了66.2%、145.1%和207.6%,Vc含量分别提高了20.8%、38.8%和61.6%。喷施100 mg/L的甘氨酸小白菜锌吸收量提高了12.0%,而喷施三种浓度的谷氨酸与苏氨酸均可提高小白菜的锌吸收量,分别平均提高了38.7%、12.0%;2）甘氨酸、谷氨酸、苏氨酸与硫酸锌混合喷施可明显提高锌的应用效果,与硫酸锌单施相比,混合喷施可分别使小白菜生物量提高25.6%、25.4%和24.5%,硝酸盐含量降低19.0%、26.3%和25.2%,可溶性糖含量提高33.2%、72.7%和27.1%,Vc含量提高67.1%、22.6%和25.2%,锌吸收量提高26.7%、50.0%和67.8%,锌利用率分别提高了14.3%、10.2%和19.2%,差异显著。【结论】 甘氨酸、谷氨酸、苏氨酸单独施用或与硫酸锌混合施用均能明显促进小白菜的生长发育及对锌的吸收利用,并能显著改善其品质。甘氨酸、谷氨酸、苏氨酸单独施用或与硫酸锌混合喷施其最适浓度范围均分别为250~400 mg/L、250~400 mg/L、100~250 mg/L。苏氨酸与硫酸锌混合喷施应用效果较甘氨酸和谷氨酸更佳,在氨基酸螯合锌肥中可优先选用。  相似文献   

9.
Metal cations were quantitatively detected by atomic absorption spectrometry in samples of olive oil mill waste waters obtained by a pressure process (omww(1)) (K, 17.1; Mg, 2.72; Ca, 2.24; Na, 0.40; Fe, 0.123; Zn, 0.0630; Mn, 0.0147; Cu, 0.00860 g L(-)(1)) and a centrifugation process (omww(2)) (K, 9.80; Mg, 1.65; Ca, 1.35; Na, 0. 162; Fe, 0.0330; Zn, 0.0301; Mn, 0.00910; Cu, 0.00980 g L(-)(1)). The inorganic anions, determined in the same samples by ion chromatography, proved to be Cl(-), H(2)PO(4)(-), F(-), SO(4)(2)(-), and NO(3)(-) (1.61, 1.05, 0.66, 0.52, and 0.023 g L(-)(1), respectively, in omww(1) and 0.61, 0.40, 0.25, 0.20, and 0.0090 g L(-)(1), respectively, in omww(2)). Most of the metal cations were revealed to be bound to the omww organic polymeric fraction (opf), composed of polysaccharides, phenol polymers, and proteins. Opf relative molecular weight was substantially estimated in the range between 1000 and 30000 Da for approximately 75% and in the range from 30000 to 100000 Da for approximately 25%. The free residual cations pool proved to be neutralized by the inorganic counteranions. Finally, the possible exploitation of this material in agriculture and in environmental biotechnology processes is also discussed in the light of its chemical and biochemical oxygen demand parameters.  相似文献   

10.
A 6-month field experiment (from October to March) was conducted in a 20-year-old experimental grove of the Aristotle University of Thessaloniki in order to enhance its micronutrient levels and assess the effect of micronutrient foliar application manganese, zinc and boron (Mn, Zn and B) on mineral nutrition, chlorophyll concentration and chlorophyll fluorescence parameters (Fv/Fm, Fv/F0 and Performance index, PI) of the olive trees (cv. “Chondrolia Chalkidikis”). The experiment consisted of four treatments (Control-C: trees sprayed with deionized water, T50: trees sprayed with 50 mg/L Mn, Zn and B, T100: trees sprayed with 100 mg/L Mn, Zn and B, T200: trees sprayed with 200 mg/L Mn, Zn and B); the three micronutrients were applied in the forms of manganese sulfate (MnSO4), zinc sulfate (ZnSO4) and boric acid (H3BO3), respectively. The results showed that the most effective treatment for the enhancement of foliar Mn and B levels was T200, while for the increase of Zn levels was T100. In addition, significant differences among the treatments were recorded for most leaf nutrient concentrations (with the exception of potassium (K), which was not influenced by foliar treatment, the other macronutrients, such as calcium (Ca), magnesium (Mg), phosphorus (P) and nitrogen (N), as well as iron (Fe), obtained their maximum concentrations, at the end of the experiment, in C or T50 treatment). Chlorophyll concentrations were not influenced by foliar treatment. Concerning chlorophyll fluorescence parameters (Fv/Fm, Fv/F0 and P index), significantly lower values were found in the control (C) trees, compared to the other three treatments, so it seems that a chlorophyll fluorescence technique may be used to detect micronutrient deficiencies in olive groves. However, from all the leaf nutrient concentrations determined, it was found that with the exceptions of: 1) Mn concentrations in the C, T50 and T100 treatments, which were marginal, or slightly deficient; 2) B and Zn concentrations in the C and T50 treatments, which were slightly deficient and marginal, respectively; 3) some K concentrations during the early spring period, which were slightly deficient, all the other concentrations were within the normal levels of sufficiency or within the optimum range, so no serious nutrient deficiency was detected.  相似文献   

11.
Abstract

Despite the importance of Zn nutrition, little information is available on the effectiveness of foliar Zn application, in general, and Zn-amino acid complexes in particular on pistachio nut yield and quality. Response of pistachio (Pistachio vera L. cv. Akbari) to foliar application of zinc (Zn) in the form of mineral (ZnSO4) and complexed with methionine (ZnMet), lysine (ZnLys), and lysine plus methionine (ZnLysMet) was investigated. Lysine alone treatment was used to distinguish the effects of accompanying Lys. A control treatment with no amino acids and Zn was also used. The critical deficiency concentration of Zn in the leaf to achieve the highest 100-kernel mass was 37.6?mg kg?1. Foliar application of Zn significantly increased leaf Zn concentration in comparison with control. In both years, foliar supply of Zn in the form of complexed with amino acids resulted in a significant increase of total nut yield, fresh mass of 100-nut, and spilled nuts. A significant decrease in the empty nuts was also observed by Zn application. The effectiveness of ZnMet in improving yield and quality attributes of pistachio was in general, higher than the other Zn sources.  相似文献   

12.
Two methods for the investigation of different types of beer by capillary zone electrophoresis are presented. The first separation system described in this work allows the quantitative analysis of beers with respect to their contents of low molecular mass anionic components using indirect ultraviolet detection as well as conductivity detection, providing relative standard deviation between 0.5 and 6.6% for the peak areas and excellent limits of detection (LOD) ranging from 0.02 mg L(-)(1) for chloride to 0.41 mg L(-)(1) for phosphate. The second method offers the possibility of fast determination of amino acids in beer samples without the necessity of any sample pretreatment. LODs obtained for the investigated solutes were found to be strongly dependent on their spectroscopical properties and in the range of 0.5-50 mg L(-)(1). Despite this restriction, this analytical method can be regarded as a suitable tool for the screening of beers with respect to their amino acid patterns.  相似文献   

13.
A test mixture of five phytohormones [naphthaleneacetic acid (NAA), naphthoxyacetic acid (NOA), indoleacetic acid (IAA), indolebutyric acid (IBA), and indolepropionic acid (IPA)] was investigated. These compounds were cleanly separated with good resolution by capillary zone electrophoresis with a UV diode array detector using 20 mM sodium phosphate buffer (pH 7.25). The lowest detection limit was obtained for IPA (0.45 mg L(-)(1) or 0.005 mg kg(-)(1)) and the highest for NAA (1.04 mg L(-)(1) or 0.014 mg kg(-)(1)). The method has been applied for tomato samples fortified with the five phytohormones using a liquid-liquid extraction procedure, obtaining recovery percentages ranging from 91 to 109.0%.  相似文献   

14.
Abstract

Foliar fertilization with micronutrients and amino acids (AAs) has been used to increase the grain yield and quality of different crops. The aim of the present study was to evaluate the effects of Zn and AAs foliar application on physiological parameters, nutritional status, yield components and grain yield of wheat-soybean intercropping under a no-till management. We used a randomized block experimental design consisting of eight treatments and four replicates. The treatments were five Zn rates (0, 1, 2, 4 and 8?kg ha?1) and 2?L ha?1 of AAs and three additional treatments: a control (without the Zn or AA application), 2?kg ha?1 Zn and 2?kg ha?1 Zn + 1?L AA. The treatments were applied by spraying during the final elongation stage and at the beginning of pre-earing for the wheat and in growth stage V6 for the soybean for two crop years in a Typic Oxisol (860?g kg?1 clay). Zinc foliar fertilization increased the wheat grain Zn concentrations. The Zn rates and AA foliar fertilization in soil with did not affect the physiological parameters, nutrient status or yield components. The AA application at the different concentrations tested changed the soybean grain yield and the leaf N concentration. The results suggest that Zn and amino acids application increases the grains Zn concentration in the wheat, being an important strategy to agronomic biofortification.  相似文献   

15.
  【目的】  基于微量元素在水稻营养和代谢中的重要作用,研究叶面喷施 (Fe EDTA 0.1%, Zn EDTA 0.05%, B 0.02%, Cu EDTA 0.05%, and Mn EDTA 0.05%) 对水稻品种 Shiroodi 产量的影响,明确叶面喷施微量元素的最佳次数和时期。  【方法】  为了准确评估液体肥料 (Fe-EDTA 0.1%, Zn-EDTA 0.05%, B 0.02%, Cu-EDTA 0.05%, and Mn-EDTA 0.05%) 对水稻品种Shiroodi产量的影响,和喷施微量元素肥料1～7次 (T1～T7) ,第一次喷施在移栽后9天进行,之后每隔7天喷施一次。  【结果】   喷施微量元素显著增加了植株干重、籽粒产量、千粒重和收获指数,而且试验年份与喷施微量元素处理对籽粒含锌量、叶片叶绿素b含量和千粒重有显著交互作用。T4处理籽粒产量为4257 kg/hm2,而 T0只有3499 kg/hm2,增产幅度约为 20%。产量和产量构成因子的提高源于喷施微量元素肥料处理增加了每株分蘖数、穗长和穗粒数。  【结论】   叶面喷施有效地提高了水稻对微量元素的吸收利用,喷施4次效果最好。  相似文献   

16.
We assessed the effects of organic fertilization on the response of biochemical and physiological indicators and the yield of saladette-type tomato (Solanum lycopersicum L.) grown under greenhouse conditions. Five fertilization forms [sand + inorganic nutrient solution (F1); sand + vermicompost tea (F2); a mixture of sand, compost, + vermicompost tea (F3); a mixture of sand, vermicompost, + vermicompost tea (F4); and a mixture of sand, compost, vermicompost, + vermicompost (F5)] and two genotypes (Cuauhtémoc and El Cid) were evaluated. The parameters analyzed were leaf pigments, enzymatic activity of nitrate reductase (NR) in vivo, and yield. A fertilizer source of sand + vermicompost tea resulted in the best assimilation of nitrate (NO3-), the greatest NR endogenous activity, the second highest foliar concentration of organic nitrogen (N), and the second best yield. In conclusion, for improved tomato cultivation during organic production, treatment F2 produced the maximum organic yield and resulted in more efficient N utilization.  相似文献   

17.
Growth and biochemical parameters of leaves, flower buds and flowers of olive (Olea europaea L. cv. Chemlali) treated by foliar biofertilization (T0: untreated trees; T1: rich in nitrogen (N), phosphorus (P), potassium (K); T2: rich in calcium (Ca); T3: application of T1 and T2) were analyzed during the flowering stage. The results showed that T1 resulted in higher contents of leaf N, K, and Na. T1 and T3 resulted in an increase of pigment concentration in leaves. All foliar treatments affected negatively the contents of total polyphenols and orthodiphenols in leaves and flower buds. Lower amounts of individual phenolic compounds were detected in the leaves under all foliar bio-fertilizations and in flower buds of olive trees sprayed with T2 and T3. This decrease is explained by the lower enzymatic activity of l-phenylalanine ammonia-lyase. In flowers, T1 exhibits the highest levels of total polyphenols, o-diphenols, and individual phenolic compounds compared to T0.  相似文献   

18.
The balance between the vitamin E (tocochromanols) and polyunsaturated fatty acid (PUFA) contents mainly determines the susceptibility to lipid peroxidation and the storage stability of corn oil. In 1997, field experiments were conducted at two different locations to evaluate a collection of 30 corn hybrids for fatty acid profiles and tocochromanol contents. Hybrids differed significantly (p < 0.01) for major fatty acids, as well as for tocochromanol contents and composition. The major fatty acids were palmitic, oleic, and linoleic acids, whose contents were in the ranges 9.2-12.1%, 19.5-30.5%, and 53.0-65.3%, respectively. The tocopherol contents ranged as follows: alpha-tocopherol, 67-276 mg (kg of oil)(-1); beta-tocopherol, 0-20 mg (kg of oil)(-1); gamma-tocopherol, 583-1048 mg (kg of oil)(-1); delta-tocopherol, 12-71 mg (kg of oil)(-1); total tocopherol, 767-1344 mg (kg of oil)(-1). gamma-Tocopherol was the predominant derivative among all tocopherols. The tocotrienol contents were in the ranges 46-89, 53-164, and 99-230 mg (kg of oil)(-1) for alpha-, gamma-, and total tocotrienol contents, respectively. The tocotrienol profile was not characterized by the predominance of any tocotrienol homologue. alpha-Tocopherol was positively correlated with PUFA (r = 0.41) and with the vitamin E equivalent (vit E equiv) (r = 0.84), and it was not correlated with gamma-tocopherol. gamma-Tocopherol was highly correlated with total tocopherol and tocochromanol contents (r = 0.93 and r = 0.90, respectively), indicating that the contribution of this vitamer to the total tocochromanol content is the most important among all tocochromanols. The high positive correlation found between the vit E/PUFA ratio and the vit E equiv, as well as the absence of correlation between this ratio and PUFA indicates that a higher vit E/PUFA ratio can be easier achieved be increasing the vitamin E content than by modifying fatty acid profile in corn oil.  相似文献   

19.
含氨基酸铜基叶面肥对芹菜产量、品质和防病效果的影响   总被引：1，自引：0，他引：1  
【目的】铜基叶面肥能够提高作物产量和品质,具有一定的防病效果;氨基酸能够为植物提供氮源,提高作物品质和抗逆能力。研究氨基酸与铜基叶面肥配合施用的效果,为制备叶面肥和改进叶面肥的施用效果提供科学依据。【方法】2015和2016年以芹菜 (Apium graveolens L.) 为试验材料进行了两季盆栽试验。设叶面喷施清水 (CK)、喷施铜基叶面肥浓度为1.0 g/L (CF1)和2.0 g/L (CF2)、喷施含氨基酸铜基叶面肥浓度为1.0 g/L (Cu-AA1) 和2.0 g/L (Cu-AA2),共5个处理,随机排列。于定植后15、45、75和105天进行喷施,喷施量依次为15、30、50和50 mL。于定植后 30、60、90和120天,测定芹菜叶片感病数,计算病情指数,用叶绿素仪测定叶片SPAD值。收获后测定了芹菜的产量、光合速率和营养品质。【结果】喷施叶面肥处理较CK显著提高了芹菜产量,在2015年Cu-AA1较CF1处理显著增产10.9%,Cu-AA2较CF2处理显著增产13.8%,CF1和CF2处理差异显著,Cu-AA1和Cu-AA2之间差异不显著。定植120天时喷施叶面肥处理的芹菜SPAD值和光合速率较CK处理显著提高,但各叶面肥处理间的SPAD差异不显著;Cu-AA1较CF1处理的光合速率显著增加了17.3%,同一叶面肥不同用量之间差异不显著。与CK相比,CF1、Cu-AA1和Cu-AA2处理的芹菜可溶性固形物、可溶性蛋白和硝酸盐含量显著增加,各叶面肥间的可溶性固形物差异不显著;Cu-AA2的可溶性蛋白较CF2显著增加,CF1与CF2之间以及Cu-AA1与Cu-AA2之间差异不显著;Cu-AA2处理的硝酸盐含量较CF2显著降低了9.9%,CF1与CF2之间以及Cu-AA1与Cu-AA2之间差异显著。定植90和120天时各叶面肥处理的芹菜病情指数较CK处理显著降低,CF1、Cu-AA1处理较CF2、Cu-AA2处理显著降低,相同用量的不同叶面肥间差异不显著。收获时,叶面肥处理的芹菜叶中的铜含量较CK显著增加,CF2较CF1显著增加了60.5%,Cu-AA2较Cu-AA1显著增加了26.4%;Cu-AA1与CF1差异不显著,Cu-AA2较CF2处理显著降低了14.0%。【结论】与普通铜基叶面肥相比,配伍氨基酸后可进一步提高芹菜生长后期叶片的SPAD值和光合速率,进一步增加芹菜产量,提高其营养品质,降低硝酸盐含量,但对芹菜的病情指数没有显著影响。在芹菜上,含氨基酸铜基叶面肥喷施浓度以1.0 g/L为好。  相似文献   

20.
Selenium (Se) is a micronutrient in mammalian nutrition and is accumulated in kale (Brassica oleracea L. var. acephala), which has high levels of lutein and beta-carotene. Selenium, lutein, and beta-carotene have important human health benefits and possess strong antioxidant properties. The objectives of this study were to determine the influence of different Se [as sodium selenate (Na(2)SeO(4)) and sodium selenite (Na(2)SeO(3))] fertility levels on (1) biomass accumulation, (2) the accumulation patterns of carotenoid pigments, and (3) elemental accumulation in the leaves of kale. Winterbor kale was greenhouse-grown using nutrient solution culture with Se treatment concentrations of 0.0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, and 3.5 mg Se/L as Na(2)SeO(4) and 0.0, 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mg Se/L as Na(2)SeO(3). Increases in either selenate (SeO(4)(-)(2)) or selenite (SeO(3)(-)(2)) resulted in decreases in kale leaf tissue biomass. Neither of the Se treatments had an effect on the accumulation of lutein or beta-carotene in leaf tissues. Increasing SeO(4)(-)(2) significantly increased the accumulation of kale leaf Se; however, leaf tissue Se did not significantly change over the SeO(3)(-)(2) treatments. Increases in SeO(4)(-)(2) affected the leaf tissue concentrations of P, K, Ca, Mg, S, B, Cu, Mn, and Mo, whereas SeO(3)(-)(2) only affected B and S. Growing kale in the presence of SeO(4)(-)(2) would result in the accumulation of high levels of tissue Se without affecting carotenoid concentrations.  相似文献   

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