共查询到19条相似文献，搜索用时 174 毫秒
1.
因具有长日照条件下雄性不育以及短日条件下可育的特性,光敏核不育水稻在两系杂交稻育种中发挥了重大作用,然而接受光周期信号决定育性转换的水稻叶片和部位尚不清楚。本研究通过在自然长日照条件下,对光敏不育水稻农垦58S(NK58S)的不同叶片和部位套袋遮光作短日处理,探讨对育性转换的敏感部位。结果表明,在育性转换敏感期,NK58S最上两个叶片接受的光照长度决定其花粉育性,其中尤以心叶对育性的贡献最大,将倒二叶及叶鞘和心叶同时遮光可增加NK58S育性恢复的稳定性。对叶片的不同部位遮光有效应差异,对叶片的上半部分遮光不能使育性转换,反之对下半部分遮光能使育性转换。另外可观察到遮光短日处理育性恢复的植株,其抽穗期比育性未恢复及整株长日处理者提前约10d。表明水稻开花的光周期和雄性育性的光周期反应有一定的关联。 相似文献
2.
水稻光（温）敏雄性不育系的育性转换机理研究 总被引：28，自引：1，他引：28
光敏核不育水稻的育性转换是由抽穗前５至１５－２０天的光温条件综合作用的结果。可育期内育性表现为一偏向高温（长日）的单峰型曲线，其数量关系可由结实率量化模型表达。温敏型不育系的育性变化由温度主控，光敏型不育系的育性在日长大于最适日长时由日长主控，短于最适日长时由温度主控，光温敏型不育系的育性由温光条件共同作用。温光当量可作为比较温度和日长对育性影响大小的具体量值。育性转换是一个可逆连续过程，其恢复和 相似文献
3.
通过不同生态点的分期播种试验,观察Ｃ４９Ｓ的生育特性及育性转换特性,发现在较大生态区范围内Ｃ４９Ｓ具有较强的育性稳定性和生态适应性。该不育系的播种至抽穗历期与纬度和海拔无明显关系。温度是诱导育性转换的主导因素,同时提出其制种和繁殖的适宜时期。 相似文献
4.
5.
光温敏核不育水稻在连云港自然条件下具有明显的育性转换期，各材料出现的时间不同，中粳，中籼型发生在８月下旬至９月初，早籼、晚粳型发生在９月上、中旬、粳型育性转换比籼型迅速、彻底。各不育系对低温，日长的反应不同，其对低温的敏感期为雌雄蕊分化期至减粉分裂期。 相似文献
6.
温光条件对龙特浦A育性影响的研究初报 总被引：7，自引：0，他引：7
龙特浦A的育性受温光条件的影响，具有温光敏特性，而温度是诱发育性转换的主导因素。掌握这一特性，扬其长，避其短，对龙特浦A的合理利用极为重要。 相似文献
7.
水稻C815S及其同源株系的育性光温特性 总被引：2，自引：0，他引：2
对C815S及其同源株系通过12种人控光温组合处理、短日遮光处理以及分期播种试验,就其育性光温特性、发育感光性以及两者之间的关系进行了研究。结果表明,C815S及其同源株系不育起点温度低,均在23℃以下。在长沙自然长日条件下没有明显不育向可育的转换;在长沙短日条件下,育性出现轻微波动。其育性受光温双因子共同作用,且这种作用的程度与发育感光性强弱似乎存在密切联系。提出了光温敏不育系光温双因子互作量化的光温效应连动假设。这一假设能提供每一光长条件下与之对应的育性转换温度,对光温敏核不育系的选育、制种和繁殖具有实际指导意义。 相似文献
8.
两个甘蓝型油菜雄性不育系RM3231A和ZCL801A在不同播期的育性变化观察 总被引：2，自引：1，他引：2
在不同播种期条件下的育性观察结果表明:RM 3231 A和ZCL801 A的有粉花朵分别从播期为8月26日的8.8%、4.8%上升至11月14日的17.4%、9.4%;有粉花朵平均育性级别分别从1.28、1.32上升至1.5、1.52;群体不育度分别从97.2、98.4下降到93.5、96.4,RM 3231 A比ZCL801 A下降更快.在不同季节播种条件下的育性观察结果表明:在9月15日秋播条件下,RM 3231 A和ZCL 801 A的有粉花朵分别为11.3%、6.1%;有粉花朵平均育性级别分别为1.37、1.39;群体不育度分别为96.1、97.9.在塘头1月28日春播条件下RM3231 A和ZCL 801 A的有粉花朵分别为86.3%、45.8%;有粉花朵平均育性级别分别为2.66、2.68;群体不育度分别为42.5、69.4.在威宁夏播条件下,RM 3231A和ZCL 801A的有粉花朵分别为81.4%、43.6%;有粉花朵平均育性级别分别为2.2、2.25;群体不育度分别为55.2、75.5.说明两不育系的不育度在秋播条件下有随播期的延迟而下降的趋势,且前者比后者下降更明显.在春播和夏播条件下两者的不育度较秋播大幅度下降,且前者下降的幅度更大. 相似文献
9.
光敏核不育水稻的光温反应研究 Ⅲ.减数分裂期温度对两个籼稻光敏不育系育性转换的影响 总被引：1，自引：0，他引：1
在人工控制条件下研究了在15小时长光照下减数分裂期的不同温度(23.3～30.3℃)对籼稻光敏不育系W6154S和5460S育性转换的影响。结果表明,温度对育性的影响因温度的高低及处理持续时间长短而异,不同的材料对温度的反应不同。5460S从不育转为可育的临界温度在26.4℃左右,而W6154S在处理温度范围内均出现自交结实现象,表明供试的W6154S株系育性转换的临界温度可能超过30.3℃。提出了深入研究影响籼稻光敏不育系育性转换的临界温度的建议。 相似文献
10.
培矮64S育性对温度与光周期的反应 总被引：8，自引：1，他引：7
在自然条件、自然温度控制光长条件及控制温度与光长条件下,研究了培矮640.0育性对温度与光周期的反应,结果表明:培矮64S在育性转换敏感期感受人工日均温21.8~24.0℃(光期23.9~24.0℃/暗期19.5~24.0℃)或自然平均日均温31.3℃(平均日最高温36.1℃,平均日最低温27.5℃)的温度,无论长日还是短日处理均表现不育,花粉可染率 相似文献
11.
小麦雄性不育育性转换相关基因TaG3BP的克隆与表达分析 总被引：1，自引：0，他引：1
为揭示YS型小麦温敏雄性不育的育性转换基础,以该类型不育系A3017不育幼穗和可育幼穗为材料构建正、反杂交SSH-cDNA文库,从可育文库中筛选出一个与G3BP(Ras-GTPase activating protein SH3 domain-binding protein)基因同源的EST序列(GenBank登录号为DY543200)。以该EST序列的同源性比对和拼接结果为依据设计引物,在可育幼穗中扩增出一条1230bp的cDNA序列。该片段含有与G3BP相似的由409个氨基酸组成的结构域,与水稻G3BP的氨基酸序列同源性为79%,被命名为TaG3BP(GenBank登录号为GU475149)。利用Real-time PCR检测TaG3BP基因在YS型小麦温敏雄性不育系花药发育各时期中的表达模式,发现该基因在育性转换的关键时期上调表达,且可育条件下的表达量高于同时期不育条件下的表达量。进一步对TaG3BP在3种不同类型的小麦K型雄性不育材料的不育系及其保持系的幼穗中的表达模式进行半定量RT-PCR分析,结果该基因在保持系幼穗中表达量较高,在不育系中表达量较低。表明该基因可能在其育性转换中具有重要作用。 相似文献
12.
棉花核不育系豫98-8A育性遗传分析 总被引：1，自引：1，他引：0
为了阐明1999年从转基因后代遗传群体中发现的1株雄性不育植株不育基因的遗传规律及其与现有不育基因的等位性,采用表型观察测量,以及经典的自交和测交手段,研究了该不育材料败育性状的遗传规律。花器官形态特征调查表明：不育株花柱长和花柱外露长度均明显高于同质系的正常可育株,而每朵花的子房直径及花药数量没有明显差异。遗传分析表明：杂合体可育株自交,后代不育株与可育株呈3:1分离,不育株与杂合姊妹可育株测交,不育株与可育株呈1:1分离,表明该核不育材料受隐性单位点控制;与阆A(msc1)、洞A(msc3)等育性位点杂合可育株分别杂交,其F1代单株育性均得到恢复。由其F1代产生的F1:2家系中均出现不育株与可育株呈1:3和7:9两个育性分离群体,表明该材料败育基因为不同于阆A、洞A的不育基因位点。 相似文献
13.
黏类小麦细胞质雄性不育相关基因cMDH的克隆与表达分析 总被引：7，自引：4，他引：3
为深入研究黏类小麦雄性不育的分子遗传机制,利用抑制差减杂交技术构建了黏类小麦育性相关基因的二核期SSH文库.经文库筛选后,得到一个在可育文库中表达的与胞质苹果酸脱氢酶基因同源的EST序列.以该EST序列为信息探针,经电子克隆、RT-PCR、PCR克隆与序列分析,获得了小麦胞质苹果酸脱氢酶(cytosolic malate dehydrogenases,cMDH)基因的cDNA与DNA序列,利用荧光定量PCR技术对该基因在不育株和可育株花药中的表达进行了分析,并比较了MDH在小麦不育株和可育株中的活性变化.结果表明,该基因的cDNA序列长1213 bp,编码333个氨基酸;DNA序列长2 908 bp,含有7个外显子和6个内含子;该基因在不育株和可育株花药发育3个时期(单核、二核和三核)的表达均表现为先升后降的模式,而且该基因在不育株花药发育的二核期和三核期的表达相对于可育株被明显抑制;MDH在小麦不育株和可育株中的活性变化趋势与定量结果一致.推测该基因在花粉发育早期表达,它的下调表达可能影响了小麦雄蕊发育过程中的能量供应而导致雄性不育. 相似文献
14.
Agro-morphological characterization of ovary culture-derived plants of rice (Oryza sativa L.) 总被引：2，自引：0，他引：2
Plants derived from unpollinated ovary culture of ten rice genotypes showed significant variability in agro-morphological
characteristics. The ovary-derived plant (H1) populations were completely haploid, doubled haploid or haploid-doubled haploid
mixture. Haploids had very drastic reduction in plant height, panicle length, grain length, breadth and number and spikelet
fertility (0.0%–2.1%). Doubled haploids from the hybrid of UPRI 95–121 × UPRI 95–165 were normal with fertility ranging between
69.6% and 97.7%. A genetic segregation in ratio of 1:1 was observed for five pigmentation characters in the H1 population
derived from hybrid UPRI 95–122 × UPRI 95–165. Plant height showed the largest coefficient of variability (28.5%) followed
by the number of spikelets per panicle (24.2%), number of grains per panicle (22.0%), percent seed set (9.2%) and panicle
length (9.0%). The range of variation in the H1 population from fully fertile hybrid PMS 2A (CMS) × IR 31802 (restorer) was
similar to its corresponding F2 population for plant height, spikelet fertility and number of grains/panicle. A single clone
of plants from the cultivar BG 1321 exhibited complete male sterility but normal female fertility when pollinated with other
varieties. Ovary-derived plants from the CMS lines PMS 2A and IR 58025A showed stable male sterility and those from thermosensitive
genetic male sterile line UPRI 95–140 showed thermosensitive genetic male sterility. These lines have potential in the hybrid
breeding program and are being currently exploited.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
15.
Summary A high frequency of male sterile mutants regeneration was shown in callus cultures derived from leaves and panicles of haploid sorghum (Msc1, A1 cytoplasm) and a spontaneous autodiploid obtained from this haploid. The cultures derived from the embryos of this autodiploid yielded significantly fewer mutants. Absolutely or partially male sterile mutants appeared among the regenerants or in the progeny of fertile regenerants. In the self-fertilized progenies of partially male sterile mutants and in the hybrids of sterile mutants with autodiploid line (i.e. under one and the same nuclear genome) male sterility mutations were inherited as cytoplasmic. Non-Mendelian segregation of sterile, partially male sterile and fertile plants was observed in these progenies. Partially male sterile plants were characterized by somatic segregation of male sterility genetic factors. In test-crosses with some CMS A1 fertility restorers, mutations were manifested as nuclear recessive while with others as nuclear dominant. These differences are supposed to be the result of interaction of fertility restorer genes of these testers with the novel cytoplasm. Male sterility mutations accompanied with female sterility were inherited as nuclear recessives.Abbreviations f
fertile
- ps
partially male sterile
- s
male sterile plants 相似文献
16.
Zhi-Wei Wang Lei Gao Hai Zhou Liu Shi Yong Mei Yuan Zhou Chang Ping Xiang Ting Wang 《Euphytica》2012,186(2):313-320
A male sterile plant appeared in the radish breeding program at the Hubei Academy of Agricultural Sciences, Hubei, China.
In its progeny, a two-type (half of plants male sterile, the other half male fertile) line 01GAB was established. An F2 population of 260 plants from a cross of male-sterile 01GAB and a male fertile line 9802H segregated for male fertility in
a 3:1 ratio indicating that fertility was restored by a single dominant gene, here designated RsMs. A PCR-based DNA marker specific to the male fertility Rfob gene in 9802H was absent in 01GAB. Linkage analysis placed the RsMs locus 10.7 cM away from the Rfo locus. In an F2 population of hybrids between 01GAB and male fertile 9802B, a co-dominant DNA marker for the RSultr3.2A (a radish sulfate transporter gene) locus was linked to the RsMs locus at 1.5 cM suggesting that fertility restoration in 01GAB was located in the region with known male sterility restorers
in radish. However, no maintainer for the 01GAB source of male sterility has been identified so far. Cytological observations
have shown that the abnormalities in male sterile anthers first appeared in tapetum at the tetrad stage, followed by a hypertrophy
of the tapetal cells at the vacuolate microspore period. These results suggest that male sterility in 01GAB is likely to be
genetic in nature, or it may represent a new type of the cytoplasmic male sterility. 相似文献
17.
Xin Liu Jiwen Zhang Cuicui Zhang Liangchao Wang Hao Chen Zengrong Zhu Jumin Tu 《Breeding Science》2015,65(4):333-339
Stem borers and leaffolders are the main pests that cause severe damage in rice (Oryza sativa L.) production worldwide. We developed the first photoperiod- and thermo-sensitive male sterility (PTSMS) rice 208S with the cry1Ab/1Ac Bacillus thuringiensis (Bt) gene, through sexual crossing with Huahui 1 (elite line with the cry1Ab/1Ac gene). The novel 208S and its hybrids presented high and stable resistance to stem borers and leaffolders, and the content of Cry1Ab/1Ac protein in chlorophyllous tissues achieved the identical level as donor and showed little accumulation in non-chlorophyllous tissue. No dominant dosage effect in the Bt gene was observed in 208S and its derived hybrids. An analysis of fertility transition traits indicated that 208S was completely sterile under long day length/high temperature, but partially fertile under short day length/low temperature. With fine grain quality and favorable combining ability, 208S had no observed negative effects on fertility and agronomic traits from Bt (cry1Ab/1Ac). Additionally, 208S as a male sterile line showed no fertility decrease caused by Bt transgenic process, as it is the case in Huahui 1. Thus, 208S has great application value in two-line hybrid production for insect resistance, and can also be used as a bridge material in rice Bt transgenic breeding. 相似文献
18.
一个与小麦雄性不育育性转换相关的MADS-box转录因子基因 总被引：2，自引：0，他引：2
为了揭示YS型小麦温敏雄性不育育性转换的基础, 构建了该类型不育系A3017的不育和可育幼穗正、反杂交的两个SSH-cDNA文库。经文库比较, 在不育文库中筛选出一个与MADS-box基因同源的EST序列(GenBank登录号: 36925702)。以该EST序列的同源性比对和拼接结果为依据, 设计引物对该基因在可育和不育幼穗中的表达进行了RT-PCR分析, 结果表明, 该基因在不育幼穗中表达量较高, 可育幼穗中表达量很低。对不育幼穗中扩增出的cDNA片段进行克隆测序, 获得了666 bp的cDNA序列。序列分析表明, 该片段编码160个氨基酸, 具有MADS-box转录因子的典型结构域K-box, 被定名为TaMS-MADSbox, 与一个小麦MADS box转录因子基因WAG的氨基酸序列的相似性为94%。进一步以3种不同类型的小麦雄性不育系和保持系的幼穗cDNA为材料, 利用半定量RT-PCR对该基因的表达模式分析发现也存在类似差异, 该基因在不育系幼穗中表达量较高, 而保持系幼穗中表达量较低。以上分析表明, 该MADS-box转录因子基因的表达与小麦雄性不育系的育性转化相关, 表达量高时表现雄性不育, 表达量低时表现雄性可育。 相似文献
19.
大豆质核互作雄性不育系NJCMS3A双亲雄性育性基因的SSR标记 总被引：3，自引：0，他引：3
利用大豆质核互作雄性不育系NJMCS3A的质、核供体亲本N21566和N21249构建F2和BC1F1育性分离群体进行雄性育性的遗传分析与基因定位。结果表明, F1正反交可育,F2和BC1F1的可育株与不育株分离比例经χ2测验分别符合3∶1和1∶1,表明NJCMS3A供体亲本雄性育性由一对基因控制,可育等位基因为显性。该基因可能是NJCMS3A的一个恢复基因。选用793对SSR引物对F2和BC1F1群体分别进行育性基因定位,发现该育性基因位于O连锁群上,在Satt331和Satt477标记之间,与Satt331、CSSR133和Satt477标记距离的次序一致,分别为8.1~10.4 cM、11.4~16.4 cM、13.3~19.2 cM。 相似文献