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1.
用假阴道采集１０只新西兰白兔的精液，经测定精子活力强，密度大，呈前进运动。然后用三羟甲基氨基甲烷缓冲液作抗冻保护，使精液在２小时内冷却至５℃，放置２天。低温保存时加入５毫克分子浓度的咖啡碱或茶碱，并在３７℃条件下持温２小时。在受胎率试验中，选用４３只新西兰白兔，每次用新鲜精液，低温冷藏精液或添加５毫克分子浓度茶碱于稀释低温保存精液输精０．５毫升（含精子１５ｘ１０~６）。结果表明，含５毫克分子浓度咖啡碱或茶碱稀释的低温保存精液，游动精子的比例增加（Ｐ＜０．０１），但持温保存时游动精子的比例降低（Ｐ＜０．０１），且精子顶体异常率增加（Ｐ＜０．０１），乳酸脱氢酶释放进入细胞间质。采用含５毫克分子浓度茶碱低温保存精液的配种效果与鲜精相同；但鲜精与冷藏精液相比，则差异显著（Ｐ＜０．０５）。采用鲜精，冷藏精液及含茶碱低温保存精液输精，母兔的产仔率分别为７０．６％，４１．７％和６４．３％． 相似文献
2.
谷胱甘肽提高牛冻精活力及受胎率的研究 总被引：6，自引：0，他引：6
冻前于牛稀释精液中添加３２ｍｍｏｌ／Ｌ还原型谷胱甘肽（ＧＳＨ）做为试验组，对照组冻精中无ＧＳＨ。结果表明：冻精刚解冻后精子活力试验组比对照组高７５％（Ｐ＜００１）；解冻后稀释精液在３７℃水浴中保存３小时后，精子活力和顶体正常精子数量试验组比对照组分别高７８％（Ｐ＜００１）和４４５％（Ｐ＜００５）；３７℃水浴条件下保存时精子寿命试验组比对照组平均延长３２ｍｉｎ（Ｐ＜００１）；此外，ＧＳＨ使母牛情期受胎率提高了６２４个百分点（Ｐ＜００５）。 相似文献
3.
稀释液溶质、渗透压、pH值和缓冲指数与低温保存鸡精液效果的关系 总被引：8，自引：0，他引：8
将葡萄糖、氯化钠、氯化钾、柠檬酸钠四种物质分别配制成三个渗透压水平(278mOsm、328mOsm、378mOsm)的12种单方精液稀释液，在低温(1～4℃)下进行鸡精液稀释保存试验，在四种物质中各选出一种好的稀释液，再将所选四种单方稀释液按不同比例制成复方精液稀释液，再次进行保存试验。所有稀释液均测定渗透压、pH值、缓冲指数、精子存活时间、精子生存指数等指标。结果表明：①柠檬酸钠、葡萄糖对鸡精液的保存具有重要作用，而氯化钾在鸡精液稀释液中并不是必需的。②复方稀释液保存效果普遍优于单方稀释液。③低温下鸡精液稀释液渗透压应为等渗或接近等渗，且最适渗透压还与构成稀释液的物质种类和相互配比有关系。④稀释液pH值应接近中性，最好与精液pH值保持一致。⑤在缓冲指数为3．894—7．257的范围内，缓冲指数与精子存活时间和生存指数呈现显著的负相关。 相似文献
4.
动物宰前体内环境ｐＨ值一般为７．１～７．２，宰后随着时间和环境温度变化，其肌肉经历了未成熟。成熟、自溶、腐败四个时期，ｐＨ值发生以下变化：７．１～７．２（宰前）→７．０～６．２（未成熟期）→６２～５．６（成熟期）→６．２～６．７（自溶期）→６．７以上即腐败期。如果宰后肉尸处理不当，出现非正常变化如酸败，ｐＨ值持续下降至３．５～５．０。品质不同，肉品ｐＨ值变化也有所不同。１ 新鲜肉 包括未成熟期（热肉期、僵硬期）和成熟期的肉品。１.１ 热肉期 宰后 １ｈ之内，ｐＨ值７．０～６.５，肉尸温度较高，血液供氧… 相似文献
5.
6.
张亚妮 《畜牧兽医科技信息》2019,(8)
本文研究了绵羊精液在高倍稀释(1:30),常温(13～15℃)条件下保存,稀释液渗透压对精子活率、精子活力、质膜完整性的影响。我们分别对照研究了两组不同渗透压稀释液,实验结果表明:在常温条件下,稀释液渗透压的pH调为6.5时,绵羊精液在较高渗透压的稀释液中保存效果较好。 相似文献
7.
应用放射免疫法测定枫泾（ＦＪ）和长白（Ｌ）青年母猪妊娠２８ｄ左右外周血清和胎儿羊水、尿囊液中硫酸雌酮（Ｅ１Ｓ）的含量；观察了两品种母猪胚胎存活数。结果，ＦＪ和Ｌ母猪血清、胎儿羊水及尿囊液中Ｅ１Ｓ含量（μｇ／Ｌ）分别为５．３±０．９９、８．０±１．０、３８．０±６．７和３．６±０．４６、５．６±０．９９、６１．４±１１．７，均处于较高水平，且两品种间无显著差异。尿囊液Ｅ１Ｓ含量极显著高于羊水和母体血清含量（Ｐ＜０．０１）；胚胎存活数（个），ＦＪ和Ｌ分别为１４．５±１．３和１０．４±１．３；血清Ｅ１Ｓ浓度与其胚胎存活数呈正相关：ｒＦＪ＝０．７４２６（Ｐ＜０．０１），ｒＬ＝０．４７４７（Ｐ＜０．０５）。上述结果表明，母猪妊娠早期孕体雌激素代谢功能很强，且以Ｅ１Ｓ形式存在于血清和胎水中。母猪血清Ｅ１Ｓ浓度不仅反映早期孕体分泌雌激素的功能，而且是预测早期胚胎存活数的有价值指标 相似文献
8.
选用半舍饲的周岁龄青海细毛羊５６只，分为三组，试验１、２组各２０只，对照组１６只。在同等饲养管理条件下，试验１、２组每只羊每日分别添加０．２ｇ和０．１ｇＲＣＦ－３稀土添加剂，进行６０ｄ饲养试验。结果：试验１、２组绵羊平均日增重分别比对照组提高３０．６％（Ｐ＜０．０１）和１２．６％（Ｐ＞０．０５）；平均羊毛长度分别比对照组提高２２．１％（Ｐ＜０．０１）和５．５％（Ｐ＞０．０５）；经济效益分别比对照组提高２５．２％（Ｐ＜０．０１）和８．７％（Ｐ＞０．０５％）。试验１组平均日增重，羊毛长度和经济效益分别比２组提高１５．９％（Ｐ＜０．０５）、１５．７％（Ｐ＜０．０５）和１５．２％（Ｐ＜０．０５）以试验１组的效果为佳。 相似文献
9.
有VP12稀释鲜精后精子存活时间及输精试验报告 总被引：1，自引：0，他引：1
ＶＢ１２注射液不仅可以延长绵羊精液在室温条件下的存活时间，而且可以提高精子活力、存活时间及配种效果。试验结果表明，试验组比对照组稀释后的有效精子存活时间提高１２．９％（Ｐ〈０．０１），配种率比对照组提高１６％（Ｐ〉０．０１），用ＶＢ１２注射液稀释绵羊精液对精子有保护作用，因此可作为绵羊鲜精稀释液。 相似文献
10.
用添加２０ｍｇ／ｋｇＲａｃｔｏｐａｍｉｎｅ的饲粮饲喂纯种大约克肥育猪，发现半腱肌ＤＮＡ含量降低１４％（Ｐ＜０．０１），ＲＮＡ含量升高７．７％（Ｐ＜０．０１），背最长肌ＤＮＡ含量下降７％（Ｐ＜０．０１），ＲＮＡ上升５．５％（Ｐ＜０．０１），且两种肌肉中的ＲＮＡ／ＤＮＡ均呈升高趋势；背最长肌中酪氨酸、苏氨酸和蛋氨酸含量分别降低２５％（Ｐ＜０．０５）、９％（Ｐ＜０．０１）和１７％（Ｐ＜０．０５），而甘氨酸和丙氨酸含量分别上升１５％（Ｐ＜０．０５）和８％（Ｐ＜０．０５），肝组织中天冬氨酸和异亮氨酸分别提高１１．６％（Ｐ＜０．０１）和１２％（Ｐ＜０．０１）；此外背最长肌肌原纤维的降解系数（ＭＦＩ）明显降低，血清尿素氮下降１６．６％（Ｐ＜０．０５）。同时还发现Ｒａｃｔｏｐａｍｉｎｅ使皮下脂肪中异柠檬酸脱氢酶活力降低２７％（Ｐ＜０．０１），血清脂肪酶活力升高１２．２％（Ｐ＜０．０５），血清胆固醇降低２３．５％（Ｐ＜０．０５）。上述结果显示：Ｒａｃｔｏｐａｍｉｎｅ可促进体内蛋白质的合成和脂肪的分解，而抑制蛋白质的降解和脂肪的合成 相似文献
11.
为提高猪精液常温保存的效果,研究不同温度和稀释液pH对猪精液常温保存效果的影响。结果表明：（1）当精子活力分别为50％和30％时,配方Ⅰ与Ⅱ稀释液保存的精液,在温度为15~20 ℃条件下的精子存活时间均显著高于21~25 ℃（P<0.05）;在pH为6.5~6.9条件下的精子存活时间均显著高于6.0~6.4（P<0.05）;（2）精子顶体染色结果表明,适宜温度与pH条件下,精液保存48 h后,顶体完整率达到96%以上。研究结果表明,猪精液常温保存适宜温度为15~20 ℃,适宜PH为6.5~6.9。 相似文献
12.
鸵鸟精液品质及精液低温保存条件的研究 总被引：1，自引：0，他引：1
测定鸵鸟精液生理指标 ,结果表明 ,鸵鸟的射精量为 0 1 7～ 2 0 3mL ,精液 pH6 7～7 3,精子密度为 1 5 7～ 48 0亿 /mL ,原精液精子畸形率 3 0 9%～ 1 3 3% ,精子活率范围0 78～ 0 92。对鸵鸟精液用不同稀释液稀释后低温保存效果比较研究 ,初步获得在 0～ 5℃时用改进MEM液作稀释液以 1∶2比例稀释后 ,精子在设计的条件下存活时间平均约 72h ,而相应原精液或 0 85 %NaCl液作 1∶2稀释的精液在同样条件下精子仅能存活约 2 4h 相似文献
13.
It has been demonstrated that after experimental infection of pig slurry from the space under the slatted floor (infection dose of 10(6)PFU per ml), the Aujeszky's disease virus (ADV) survived for 72 hours at the temperature of 15 degrees C and at pH 6.5, but was inactivated after 96 hours. When technologically treated pig slurry from the storage tanks was saturated with water and infected with ADV at the dose of 10(5)PFU per ml, the virus survived for 23 days when kept at 15 degrees C and 4 degrees C and at pH 6.8, but was inactivated under the same conditions after 30 days. When the infective ADV dose in the technologically treated pig slurry in the storage tanks was reduced to 10(4)PFU per ml, the virus survived 16 days at +4 degrees C and pH 7.0 and 8.0 but was inactivated within 23 days after infection. 相似文献
14.
旨在评价酵母培养物(YC)对公猪生产性能的影响。将90头体重约为80kg的公猪随机分为3组,即对照组、试验Ⅰ组和试验Ⅱ组;对照组饲喂基础日粮,试验Ⅰ组和试验Ⅱ组基础日粮中分别添加5kg/t和7kg/t的酵母培养物,预试期5d,正试期45d,试验期间,3组饲养管理条件一致;试验结束时,评定各组公猪精液的颜色、气味,测定并比较各组公猪的精液量、精液pH值、精子活力、精子密度、精子畸形率、抗力指数。结果表明,试验Ⅰ组和试验Ⅱ组公猪的精液颜色和精液气味与对照组相比没有明显差异;3组公猪的精子抗力指数一致;试验Ⅰ组和试验Ⅱ组的精液量、精子活力、精子密度均显著大于对照组(P<0.05),精子畸形率均显著小于对照组(P<0.05),但这4项指标在2个试验组之间差异均不显著(P>0.05);3组公猪的精液pH值差异均不显著(P>0.05)。综上提示,基础日粮中添加一定量的酵母培养物能够提高公猪部分生产性能指标。 相似文献
15.
The acid-base status of 58 calves (all in normal anterior presentation) was determined from venous blood samples before the onset of traction and immediately after vaginal delivery. Calves were assigned to one of three groups according to their blood pH value: group 1 - normal, pH greater than 7.2; group 2 - acidotic, pH 7.2 to 7.0; group 3 - severely acidotic, pH less than 7.0. Before the onset of traction (i.e. during the 30 minutes following the appearance and rupture of the membranes), 43 calves (74.1%) had normal acid-base values, 14 (24.1%) had slight acidosis, and only 1 (1.7%) had severe acidosis. At birth the three groups of calves showed the following distribution: 23 (39.7%) were normal, 29 (50%) had slight acidosis, and 6 (10.3%) had severe acidosis. Seven calves (12.1%) died during birth or within 48 hours after birth; 2 were normal shortly before birth, 4 were acidotic shortly before birth, and 1 was severely acidotic even before the onset of traction. Traction was significantly longer for cows that delivered severely acidotic calves compared to cows in the other two groups. 相似文献
16.
André M. Crespilho Beth E. Spizziri Mindy Meyers James K. Graham 《Journal of Equine Veterinary Science》2013
Cooled stallion semen has a short viable life, which ranges with acceptable motility and viability from 24 up to 48 hours. The purpose of this study was to compare the effects of storage pH, the ability of three different zwitterionic buffers, and cholesterol-loaded cyclodextrins (CLC) to preserve the motility and integrity of stallion sperm cooled to 5°C for 48 hours. Fourteen ejaculates were collected and split to receive CLC or not (control group). After incubation, each sample was split into six subsamples and diluted in KMT extender containing 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), or 2-(N-morpholino)ethanesulfonic acid (MES) buffers, and the final pH was adjusted to either 7.0 or 6.6, totalizing 12 experimental groups as a function of CLC, buffer, and pH variables (2 × 3 × 2 factorial). The motility parameters and integrity of plasma and acrosome membranes (live cell index) were determined using computer-automated semen analysis and epifluorescence microscopy at 3, 6, 24, and 48 hours of cooling period. According to results, pH was not a significant source of variation for motility and live sperm over different cooling periods. However, samples diluted in BES exhibited higher progressive motility within 3 hours and higher percentages of total motile cells after 48 hours of incubation at 5°C (P < .05). After 24 hours of storage, CLC-treated sperm samples presented higher motility than control group (P < .05), and after 48 hours of incubation, CLC-treated sperm exhibited higher percentages of live, motile, and progressively motile sperms (P < .05). We inferred that equine semen diluted in KMT containing BES as buffer and CLC treatment improve the equine sperm survival during storage at 5°C for 48 hours. 相似文献
17.
稀释液溶质和渗透压对鸡精液低温保存效果的影响 总被引：1，自引：1，他引：0
观察15种物质不同渗透压（300mOsm、400mOsm、500mOsm）对鸡精子低温保存的精子活力、生存指数、存活时间和30h精子畸形率的影响。结果表明,对鸡精子低温保存生存指数大于50的物质由高到低依次为果糖、甘露醇、谷氨酸钠、山梨醇和氯化钾;精子总存活时间大于120h的物质由高到低依次是果糖、甘露醇、谷氨酸钠、山梨醇、氯化钾、磷檬酸钠和乙酸钾;30h精子畸形率小于0．2的物质由低到高依次为L-谷氨酸钠、甘露醇、蔗糖、乳糖。渗透压分析结果表明,各指标均随渗透压升高而降低。在300mOsm时,30h精子畸形率、精子生存指数和存活时间均极显著高。 相似文献
18.
The objective was to assess the influence of polyunsaturated fatty acid supplementation on the quality of fresh, cooled, and frozen-thawed stallion semen. Ten stallions received their normal diet (control group) or normal diet plus 150 mL of polyunsaturated fatty acid (PUFA) linseed-based oil (PUFA group). Semen was collected every 15 days during 60 days. Stallions were reversed across the treatments after a sixty-day interval. Semen was evaluated at 2, 6, 12, and 24 hours after cooling and 24 hours after freezing. Motility (MOT), vigor, membrane viability, morphology, acrosome integrity, and osmotic tolerance test (OTT) were evaluated. In the frozen-thawed semen, sperm dynamic characteristics were analyzed by computer-assisted sperm analysis and thiobarbituric acid reactive substances (TBARs) determined. The effects of treatment, time, semen type, and their interactions were submitted to PROCMIX (SAS) and means compared by the Tukey test. There was no treatment effect on the quality of fresh and cooled semen. However, frozen-thawed semen MOT, vigor, and OTT were superior (P < .05) in control compared to PUFA group. An interactive effect of sample day by treatment was observed, such that, TBARs increased over time (P = .002) in the PUFA group after 15, 30, 45, and 60 days from the beginning of supplementation. Thus, sperm may become more susceptible to the reactive oxygen species, probably due to the incorporation of polyunsaturated fat in the cell membrane. The addition of PUFA-enriched oil may be an alternative for improving frozen-thawed semen quality by increasing its MOT and resistance to osmotic changes to which sperm cells are submitted during the freezing process. 相似文献
19.
中国奶年饲养标准科研协作组 《中国畜牧杂志》1987,(2)
为建立我国根据牛奶营养成分推算所含能值的公式,中国奶牛饲养标准科研协作组组织全国六单位协作,在全国范围内采集有代表性的牛奶样本475,对其中的总干物质、乳脂肪、乳蛋白、乳糖、和能量等项进测定,并对所得结果分别进行多元和一元回归分析,所得主要回归方程如下: 相似文献
20.
Effect of a hay and a grain diet on the rate of hydrolysis of ochratoxin A in the rumen of sheep 总被引：2，自引：0，他引：2
H Xiao R R Marquardt A A Frohlich G D Phillips T G Vitti 《Journal of animal science》1991,69(9):3706-3714
The hydrolysis of ochratoxin A (OA) and the corresponding formation of its hydrolysis product, alpha ochratoxin (O alpha), by ruminal digesta and in the rumen of hay-fed and grain-fed sheep were compared. Ruminal contents from sheep fed diets with hay or with grain hydrolyzed OA in vitro; the majority of the activity was associated with the particulate fraction of the ruminal contents. The rate of hydrolysis of OA by ruminal fluid that was adjusted to different pH values was not influenced (P greater than .6) by the pH of the samples (pH was from 5.5 to 7.0). Ruminal fluid obtained from hay-fed animals (pH 7.0) was able to hydrolyze OA in vitro and to produce the hydrolyzed product, O alpha, at a much greater rate (fivefold) than ruminal fluid obtained from grain-fed animals (pH 5.5) (P less than .01). Ochratoxin A was administered intraruminally at a concentration of .5 mg/kg of BW to hay-fed and grain-fed sheep. The half-lives for disappearance of OA from the rumen of sheep fed grain (normal feed intake, rumen pH 5.7), fed grain at a low level (30% of normal feed intake, pH 6.5), and fed hay (pH 7.1) were 3.6, 1.3, and .6 h, respectively. The results suggest that OA is hydrolyzed much faster in the rumen of sheep fed hay than in sheep fed grain, presumably because of the different ruminal microbial population, which in turn influenced the rate of hydrolysis of OA. 相似文献