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不同油豆角品种过氧化物酶活性及其同工酶酶谱分析 总被引：1，自引：1，他引：0
应用过氧化物酶同工酶技术,通过相似系数比较和聚类分析,比较7个油豆角品种不同器官过氧化物酶(POD)的活性,并对其亲缘关系进行探讨。结果表明:不同品种POD活性不同,同一品种不同器官的POD活性也不同,根部的POD活性高于叶的,而叶片的高于茎的;不同器官POD酶谱不同,同一器官不同品种的酶带数也不尽相同;‘油豆子’、‘俏春大荚油豆王’和‘齐研五号架油豆’亲缘关系比较近;‘俏春3号地油’与‘油豆子’的亲缘关系最远。 相似文献
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海南省优良油豆角品种引种比较试验 总被引：3，自引：0，他引：3
为筛选出适合海南岛反季节种植的优良油豆角品种,对引种的榆树大油豆、紫花油豆、九月青、一棵树、57号架油豆、将军豆等6个油豆角品种进行了品种比较.通过对适应性、植物学性状、产量、嫩荚的性状、抗病性的调查分析表明,6个油豆角品种均能在海南岛顺利完成整个生长发育过程;榆树大油豆产量最高(20 010 kg/hm2),抗病性最强,商品品质较好,可以作为中晚熟的优良品种进行推广;而将军豆产量虽低(15 050 kg/hm2),但其单荚重最大(19.67 g),抗病性最强,商品品质最好,紫花油豆产量较高(19 630 kg/hm2),外观商品性好,2个品种也可作为中、早熟品种在海南进行推广. 相似文献
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紫花油豆是我国东北地区具有鲜明区域特色的当家蔬菜品种之一。紫花油豆为蔓生种,植株生长势强,基部分枝多,三出复叶中等大小,卵圆形;总状花序,花为紫色,在主蔓上6～7节着生第1花序;嫩荚 相似文献
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"龙油豆一号"由黑花油豆选育而成,2001年通过黑龙江省品种审定委员会审定并命名.该品种蔓生,早熟,生育期65 d(天),株高2.5 m～3.2 m(米),生长势强,抗病性好,尤其抗病毒病,耐炭疽病,产量高于对照品种紫花油豆.豆荚扁条形,绿色,架面有光泽,典型的油豆角.豆角荚长12 cm～15 cm(厘米),荚宽1.8 cm～2.0 cm(厘米),纤维少,肉质细腻,风味极佳,商品性好,还可以速冻贮藏长期食用,适于保护地和露地栽培. 相似文献
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菜豆大棚早熟栽培品种筛选 总被引：1，自引：1，他引：0
对紫花油豆、一粒王油豆、吉林油豆、三叶油豆、冠军油豆、一棵树油豆、五常油豆等10个菜豆品种进行了大棚春播栽培,通过对其植物学性状、抗病虫性、品质和产量的调查分析结果表明,维生素C含量最高的品种为五常油豆、八月绿和一粒王油豆,蛋白质含量最高的品种为五常油豆,纤维素含量最低的品种为八月绿,产量最高的品种为冠军油豆,发病率最低的品种为八月绿和一棵树油豆.品质试验综合分析表明在大棚早春栽培过程中五常油豆和八月绿油豆均表现出维生素C含量较高、蛋白质含量较高、纤维素含量较低,发病率较低,生长势旺盛和产量中等等特点,可作为大棚春播菜豆首选品种加以推广.冠军油豆产量很高,熟性适中,中后期长势较旺盛,品质略低于五常油豆和八月绿,但综合性状较好,也可作为早春菜豆主栽品种加以推广. 相似文献
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1品种简介 蓝色架莱豆品种是大连市农业科学研究院1998年从以色列引进的菜豆品种，商品荚紫色、圆形、荚长20厘米左有、中筋、无革质膜、口感好、产量高、耐贮运。中熟，辽南地区春露地比连农923品种晚熟5—7天，结荚期长，再生能力强。适应范围广。 相似文献
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以"紫花"、"亮秋"、"将军豆"、"龙油豆一号"、"龙油豆三号"、"龙油豆四号"、"冠军"、"一棵树"、"黑豆黄"、"春秋绿"、"黄金钩"、"八月绿"和"黑珍珠"13个油豆角品种为试材,比较分析了13个油豆角品种的生物学特性、产量和嫩荚性状等,以期筛选出适合青海省西宁地区露地栽培的优良油豆角品种。结果表明:"龙油豆三号"、"龙油豆四号"和"将军豆"产量较高,商品性较好,可作为适宜品种在西宁地区推广种植。 相似文献
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选取前期试验中筛选的坪用价值优异的狗牙根野生资源为研究材料,采用二因素裂区试验小区建坪,模拟足球场草坪的运动受力进行滚压处理,研究不同滚压强度对野生狗牙根的影响及不同材料间表现的差异.试验结果表明:不同滚压处理对狗牙根草坪的盖度、地上生物量、分蘖数、单位面积粗纤维、叶绿素和可溶性糖含量都存在不同程度的影响作用.不同材料之间也存在差异:生物学特征方面:嘉陵江流域的J7的草坪盖度和分蘖数均高于对照Jackpot,岷江流域的M28、M22两份材料次之,青衣江流域的Q10表现最差;生理学特征方面:单位面积的粗纤维、叶绿素、可溶性糖含量均是J7表现最好,其含量均高于对照Jackpot,其次是M22和M28,Q10最低. 相似文献
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检测了编号为M1、M2、M7、M8、10#羊肚菌菌丝体在液体培养基中生长时胞外酶的活性及培养液中糖与蛋白质的含量,同时观察了菌丝体生长情况。结果表明:菌丝体生长情况各不相同,10#在菌丝片的产生及菌丝成团最早,依次为10#M8M7=M2M1;与碳水化合物降解相关的酶(纤维素酶、淀粉酶)活性变化趋势基本相同,在发酵的第2~3天出现第1个峰值,并于第6天出现第2个峰值,且第1个峰值大于第2个峰值;在整个培养期内漆酶、愈创木酚酶、蛋白酶均有活性,产酶高峰也不尽相同;蛋白质、糖含量随时间变化且与酶活力相关。 相似文献
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ZHANG Min HUANG Xiao-yan ZHOU Xi SHI Xiang-xiang HUANG Fang ZHANG Huai-qin YANG De-ye 《园艺学报》2010,26(3):504-508
AIM: To investigate the effect of selective silencing of SLC7a8 on uptaking L-dopa in renal tubular epithelial cells of rat (NRK-52E). METHODS: The three siRNAs targeting SLC7a8 (siRNA-1, siRNA-2, siRNA-3) were designed and synthesized. A siRNA with nonspecific coding sequence (siRNA-con) was used for control. All siRNAs were transfected into NRK-52E cells. The siRNA-con transfected group, blank control group and gene-specific silencing SLC7a8 group were set up. The efficiency of transfection was estimated by flow cytometry. The efficiency of RNA interference was detected and screened by RT-PCR preliminarily, and was followed by Western blotting at protein level. The concentrations of L-dopa uptake into the NRK-52E cells were detected by ultraviolet spectrophotometry at different time points (6, 12, 24, 36, 48, 60, 72 and 120 min). RESULTS: The transfection efficiency was 94% detected by flow cytometry. The initial screening of RT-PCR showed that the efficiencies of RNA interference of siRNA-1 and siRNA-3 were higher, and siRNA-3 was the highest at protein level determined by Western blotting. No distinctive change was found between siRNA-con treated NRK-52E and blank control cells. The L-dopa uptake at different time points (6, 12, 24, 36, 48, 60, 72 and 120min) in siRNA-interference group was lower than that in siRNA-con transfected group and blank control group. No significant difference of L-dopa uptake between siRNA-con group and blank control group was observed. CONCLUSION: RNA interference technology selectively down-regulates SLC7a8 expression in rat renal tubular epithelial cells. The L-dopa uptake is also decreased after specifically silencing the slc7a8 expression. 相似文献
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Andreas Spornberger Elisabeth Schüller Edina Vidéki György Végvári 《Erwerbs-Obstbau》2018,60(3):231-238
In autumn 2008, in the research orchard of the University of Natural Resources and Life Sciences in Vienna, the apple cultivar ‘Topaz’ was planted on the rootstocks M9 (clone T337) with and without ‘Rubinola’ as interstem, M26, M7 grafted at 25?cm and at 55?cm respectively, M111 and ‘Bittenfelder seedling’, trained as spindles and tested for eight years according to organic production rules. The trees on seeding grew very vigorously, followed by MM111 and M7 normally grafted; the M7 highly grafted trees showed less vigor, comparable with M26, while the trees on M9 with and without interstem grew very weakly. Tree losses were only observed on M9 without interstem. The highest yields per hectare were found on M7 normally grafted, followed by M26, M7 highly grafted and M9 without interstem. M7 highly grafted showed the highest single fruit weights, followed by MM111, at the other end were M9 with and without interstem, and M26. The rootstock M7 grafted at 55?cm combines a medium growth with high yield and good fruit quality and therefore can be recommended for dry areas like our site. M9 without interstem showed a higher specific yield and fruit firmness after storage compared to the variant with interstem. Whether these advantages for ‘Topaz’ on M9 can be combined with a higher grafting height as effective preventive method to control Phytophthora cactorum, has to be examined in further research. 相似文献
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SU Cheng-cheng ZHANG Yi-dan MA Yong-qiang CHEN Xue-fen XIANG Guo-an ZHOU Xin PENG Shou-chun LIN Zhi-chun WEI Lu-qing JI Wen-jie 《园艺学报》2015,31(11):2065-2069
AIM: To establish a cell line of stable silencing of P2X7 receptor (P2X7R) expression through short hairpin RNA (shRNA)-mediated interference in murine RAW264.7 macrophages, and to investigate the proliferation and apoptosis in the cell line. METHODS: Stable silencing of P2X7R gene in the RAW264.7 cells was achieved by recombinant shRNA plasmid targeting murine P2X7R gene via liposome mediated transfection, followed by G418 selection. The efficacy of plasmid transfection and P2X7R silencing in G418 resistant cells was verified by immunofluorescent microscopy and real-time PCR, respectively. The proliferative activity was analyzed by CCK-8 assay and EdU cell proliferation assay. The cell cycle distribution and apoptosis were evaluated by flow cytometry. RESULTS: The expression of P2X7R at mRNA and protein levels was down-regulated by 80% in sh P2X7R group compared with negative control (NC) plasmid transfection. In addition, P2X7R-silencing cells exhibited higher proliferative activity compared with NC and wild-type RAW264.7 cells (P<0.05). Compared with NC cells, P2X7R silencing resulted in an increase in the phagocytosis of the cells (P<0.05). CONCLUSION: A cell line RAW264.7 of stable silencing of P2X7R expression was successfully established. P2X7R gene silencing stimulates the proliferation, and changes phagocytic function in murine RAW264.7 macrophages. 相似文献
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Samples of the ‘Ashley’, ‘Hartley’ and ‘Franquette’ cultivars of English walnut (Juglans regia L.) were collected at weekly intervals from bloom to harvest, and development of the fruit, embryo and surrounding tissue was followed. The first division of the zygote occurred approximately one week after pollination, by which time the endosperm, nucellus and integument had shown considerable growth. The endosperm first formed cell walls at the 8-celled embryo stage and was completely cellular by the time the embryo contained approximately 64 cells. Rapid growth of the cotyledons started as the shell began to harden at Week 7. Measurement of fruit growth indicated that fresh weight gain in ‘Ashley’ and ‘Hartley’ fruits followed a double sigmoid pattern, characterized by a 3-week period of slowed growth that began approximately 7 weeks after bloom. Rate of increase in total length and diameter also slowed greatly at Week 7. Fresh weight gain in ‘Franquette’ appeared to follow a single sigmoid curve, with no prolonged period of slowed growth corresponding to that in the other cultivars. Kernel growth also described a double sigmoid curve, with its initial, rapid phase nearly coincident with the beginning of Stage III of the whole-fruit, fresh-weight curve at Week 10. 相似文献
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对印度南瓜（Cucurbita maxima）开展转录组测序分析,共获得131 960条unigene（90.80 Mb）,筛选得到12 557个SSR位点（占总unigene的9.52%）,其发生频率为1/7.4 kb;其中SSR位点中主导类型为二核苷酸重复,占总SSR的49.82%;其次是三核苷酸重复,其出现频率为45.31%。通过Primer5设计得到7 290对SSR引物,随机选择20对SSR引物对30种不同来源的南瓜进行多态性验证分析,结果显示在14对可扩增产物的SSR引物中,11对引物表现稳定可重复的多态性,UPGMA多态性分析显示11对引物可将30份南瓜材料分为5类。研究表明通过对南瓜转录组分析可获得较高频率的SSR位点且类型丰富,为印度南瓜遗传多样性分析和遗传图谱构建提供了候选标记。 相似文献
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番茄子叶总蛋白双向电泳体系的建立 总被引：9，自引：0，他引：9
通过对IPG胶条梯度、上样方式、上样量、聚焦条件等4方面条件的优化,建立了番茄子叶总蛋白双向电泳体系,即采用TCA丙酮沉淀法提取番茄子叶总蛋白,选用24 cm pH3-7NL的IPG胶条,采用水化上样,上样量300 μg,按聚焦程序Ⅰ或Ⅱ聚焦后进行双向电泳分离和银染染色。若采用制备胶,以获取高含量蛋白点,则将上样量提高至1.5 mg,按聚焦程序Ⅲ进行聚焦后进行双向分离,并采用胶体考马斯亮蓝染色。采用该优化的体系可以获得分辨率高、重复性好的双向电泳图谱。 相似文献