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1.
AIM: Preparation and identification of McAbs against synthetic human inhibin α、βA、 or βB fragments and its immunohistochomical application in cancers.METHODS: The fragment of inhibin subunits α(1-26)(37-65), βA(1-28)(82-114), βΒ(1-28)(85-115) have been synthesized. Inhibin isolated from porcine follicle fluid and synthetic polypeptide fragments were used as antigen. Intraperitoneal immunization was performed on BALB/c mice, then boosted with intraspleen injection was performed 3 days prior to fusion. After fusion, selection and cloning procedures, 14 hybridoma cell lines were successfully obtained and their immunological characteristics were analyzed. After purification, McAbs were applied to immunohistochemical staining in gonadal tumors and other cancers.RESULTS: We obstained 14 stable cell lines which can secret McAbs againsting INH α、βA or βB subunit. The titre of ascites were 1×10-4-1×10-6. Immunohistochemical positive staining were observed in ovarian granulosa cell tumor (100%), ovarian mucinos tumor, embryonal carcinoma of the ovary, testis spermocytoma, embryonal carcinoma of the testis, In 40 samples of breast cancers, each INH subunit positive rates were over 70%.CONCLUSION: The INH's McAbs prepared by ourselves were usefull for cancer immunohistochemistry.  相似文献   

2.
AIM：To investigate the effects of fenofibrate on angiotensin Ⅱ (AngⅡ)-induced cardiomyocyte hypertrophy. METHODS：Primary neonatal cardiomyocytes were pretreated with fenofibrate (10 μmol/L) for 1 h followed by stimulation with AngⅡ (100 nmol/L). The mRNA levels of ANF, BNP and β-MHC were measured by real-time PCR. Western blotting was employed to determine the nuclear translocations of NFATc4 and p65-NFκB. Co-immunoprecipitation was used to investigate the interaction of NFATc4 with p65-NFκB in the nucleus of cardiomyocytes. In addition, the DNA binding activity of NFATc4 on the BNP promoter was determined by EMSA. RESULTS：Fenofibrate significantly inhibited AngⅡ-induced cardiomyocyte hypertrophy. Fenofibrate treatment inhibited the nuclear translocations of NFATc4 and p65-NFκB, as well as the interactions of NFATc4 with p65-NFκB in the nucleus of cardiomyocytes induced by AngⅡ. Fenofibrate inhibited the binding activity of NFATc4 with the BNP promoter, which was strengthened by AngⅡ. CONCLUSION： Fenofibrate enhances the interaction of NFATc4 with PPARα, decreases the interaction of NFATc4 with p65-NFκB in the nucleus of cardiomyocytes, and inhibits the DNA binding activity of NFATc4 induced by AngⅡ, which may be the important mechanisms of fenofibrate on inhibiting cardiac hypertrophy.  相似文献   

3.
Protection of biodiversity and ecosystem functions requires a better understanding of spatial diversity. Here we studied diversity patterns of true bugs and saproxylic beetles, sampled in 28 forest stands of southern Germany, using a hierarchical nested design of five increasingly broader spatial levels: trap location, stratum, forest stand, forest site, and ecoregion. We predicted that: (1) for large body-sized species (as a surrogate for highly mobile species) and host generalist species (low host specificity), the proportion of β-diversity decreases from small to large spatial scales; and (2) the differences between trait-based functional guilds in the proportion of β-diversity increase with increasing weighting of more-abundant species. Our results indicated that the ecoregion level is the most important diversity scale for both taxa and among functional guilds sampled, followed by the forest stand level. Specialized species were more strongly affected on the ecoregion level than generalist species. Differences in the proportion of β-diversity between functional guilds increased with increasing weighting of abundant species. The β-diversity patterns based on body size and host specificity were similar for true bugs, but partly contrasting for saproxylic beetles. Our results suggest that (1) future conservation schemes should focus on establishing new conservation sites in new ecoregions, rather than on enlarging existing protected areas; (2) host specificity might be a more meaningful trait than body size to be considered in biodiversity studies; and (3) common conservation approaches restricted to only large, conspicuous, but rare species might result in a mismatch of important biodiversity scales.  相似文献   

4.
This study established a highly effective micropropagation system to obtain good plantlet proliferation from floral organs via callus induction and bud differentiation in Guzmania ‘Hilda’ bromeliad. The best frequencies of organogenic callus formation (20% in petal and 35% in ovary explants) were obtained on media containing a combination of 1.0 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) with 1.0 mg l−1 α-naphthaleneacetic acid (NAA) and 1.5 mg l−1 2,4-D with 0.5 mg l−1 NAA, respectively. Organogenic calli were cultured on medium with 1.0 mg l−1 NAA and 0.5 mg l−1 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (TDZ) induce the differentiation and regeneration of adventitious buds into plantlets. When the plantlets were cultured in a medium with optimum NAA concentration (0.5–1.0 mg l−1) significant improvement in regeneration and elongation was achieved within one month. This overcame the difficulty of delayed elongation in Guzmania plantlets. More than 99% of the regenerated and acclimatized plantlets developed to the flowering stage.  相似文献   

5.
AIM: To evaluate the effect of interfering TGF-β receptor Ⅱ (TβRⅡ) expression on the viability and differentiation of human acute promyelocytic leukemia NB4 cells induced by all-trans retinoic acid (ATRA) and their apoptosis induced by arsenic trioxide (ATO). METHODS: The technique of lentivirus-mediated RNA interference was used to obtain stable NB4 cells with TβRⅡ knockdown, named TβRⅡ-shRNA NB4 cells. CCK-8 assay was used to detect the viability of TβRⅡ-shRNA NB4 cells. The expression level of CD11b was analyzed by flow cytometry, and Wright-Giemsa staining was used to detect the effects of ATRA on the differentiation of TβRⅡ-shRNA NB4 cells. Double staining (Annexin V-FITC/PI) and AO/EB staining were used to detect the effects of ATO on the apoptosis of TβRⅡ-shRNA NB4 cells. RESULTS: The viability of TβRⅡ-shRNA NB4 cells was significantly higher than that of NB4 parental cells. The differentiation was induced in TβRⅡ-shRNA NB4 cells and NB4 parent cells by treatment with ATRA at different concentration (0.01, 0.02, 0.04, 0.08, 0.1 μmol/L) for 96 h. The differentiation rate of TβRⅡ-shRNA NB4 cells was lower than that of NB4 parental cells in a dose-dependent manner. ATO induced apoptosis of TβRⅡ-shRNA NB4 cells and NB4 parent cells at different concentrations (2, 4 and 8 μmol/L) for 24 h. The apoptotic rate of TβRⅡ-shRNA NB4 cells was lower than that of NB4 parental cells dose-dependently. At the concentration of 8 μmol/L for 24 h, the apoptotic rates in TβRⅡ-shRNA NB4 cells and NB4 cells were (49.15±2.05)% and (66.85±2.41)%, respectively (P<0.01). CONCLUSION: Down-regulation of TβRⅡ increases the viability of NB4 cells, inhibits NB4 cell differentiation induced by ATRA, and also inhibits apoptosis induced by ATO.  相似文献   

6.
Avocado production is dependent on the singular synchronous protogynous dichogamy of the species that promotes outcrossing. With the objective of selecting potential pollinizer avocado genotypes for ‘Hass’, the most important avocado cultivar worldwide, we have monitored during two consecutive years the flowering phenology of 27 avocado genotypes in South-eastern Spain. The average length of the flowering season was 45 days ranging from 18 days for ‘Harvest’ to 50 days for ‘Fuerte’. The earliest genotypes to flower were ‘Fuerte’ and ‘Shepard’ that started blooming during the third week of March. The latest genotypes to flower were ‘Colin V-33’, ‘Adi’, ‘OA184’ and ‘Harvest’, which started blooming in the second week of April. ‘Hass’ blooming lasted 30 days, from the first week of April until the second week of May. Since a good pollinizer must present not only an overlapping in the flowering season but also an overlapping in sexual stages with the pollinated cultivar, a group of 12 genotypes (‘Hass’, ‘Fuerte’ and 10 genotypes producing ‘Hass-like’ fruit with good overlapping in the flowering season with ‘Hass’) was studied with more detail determining daily the stages of male and female overlapping every 2 h. Results herein indicate that ‘Marvel’ and ‘Nobel’ showed a high sexual overlapping with ‘Hass’. Taking into account the flowering phenology, the overlapping in sexual stages and the fruit set obtained with hand-pollinated flowers in the field, those two genotypes could be an interesting alternative to the current use of ‘Fuerte’ as pollinizer for ‘Hass’ in South-eastern Spain.  相似文献   

7.
Utilization from bio fertilization is well known a considerable tool to improve the yield and fruit quality of various crop fruits through the increasing emphasis on maintain of soil health, minimize environmental pollution and decrease the use of chemical fertilization. In this study, in order to improve wine grape quality features of cv. Shiraz, four different doses of foliar microbial fertilizer, including 0, 1000, 2000 and 3000?ppm were applied at two different terms as Term I (mostly; pre-bloom applications) and Term II (mostly; post-bloom applications). However, there were no influences of application terms of foliar microbial fertilizer treatments; treatment doses had considerable effects on yield and quality parameters. The lowest p-values, meaning the highest berry quality, from doses of foliar microbial fertilizer were obtained from 2000?ppm (105.08?μW), 1000?ppm (110.40?μW), 3000?ppm (112.97?μW) and 0?ppm (119.58?μW). Comparing the applications of foliar microbial fertilizer, it was observed that doses of 2000?ppm (3155.56?mg/kg), 1000?ppm (3000.92?mg/kg) and 3000?ppm (2530.19?mg/kg) exhibited higher total phenolic compounds content when compared with 0?ppm treatment (2206.97?mg/kg). Berries from grapevines applied with the doses of 2000, 1000 and 3000?ppm foliar microbial fertilizer respectively shown higher total anthocyanin content such as 1230.19, 1160.85 and 865.86?mg/kg compared to 0?ppm (637.37?mg/kg). As a result, research the findings indicated that 2000 and 1000?ppm doses of foliar microbial fertilizer were obviously effective on wine grape quality features of cv. Shiraz in terms of electrochemical property, total phenolic compounds content and total anthocyanin content.  相似文献   

8.
Uneven grape ripening, leading to decreases economic valued of grapes is one of the main problems faced by grape growers in early table grape growing. In recent years, there has been increasing concern about improving of fruit quality in fruit crops. Among the various quality improvement methods, different types of foliar compounds are used for this purpose and hydrogen peroxide (H2O2) and phenylalanine (Phe) can be given as examples to some of these chemicals. This research was carried out during the 2015 growing season by using early ripening table grape cv. Trakya Ilkeren. In the study, it was assessed that how different doses of hydrogen peroxide (0, 10, 20 and 40?mM) and phenylalanine (0, 1, 2 and 4?mM) affected physical and biochemical properties of cv. Trakya Ilkeren. In terms of physical properties, doses of hydrogen peroxide and phenylalanine had only significant effects on grape firmness and cluster length. The best results were obtained from 4?mM Phe application (6.76?N) in grape firmness and were obtained from applications of 10, 20 and 40?mM H2O2 (23.66, 23.86 and 23.99?cm) and applications of 1, 2, and 4?mM Phe (23.95, 24.46 and 24.88?cm) in cluster length. However, most of biochemical properties were considerably affected by doses of hydrogen peroxide and phenylalanine except for juice pH and p-value. It was recorded that the highest total phenolic compounds contents varied from 3571.28 (10?mM H2O2) to 3871.22?mg GAE kg?1 fw (40?mM H2O2) compared to 0?mM application (2591.48?mg GAE kg?1 fw). In terms of total anthocyanin content, the highest mean was recorded for 4?mM Phe application (330.56?mg GAE kg?1 fw) than 0?mM application (168.79?mg GAE kg?1 fw). As a result, 4?mM Phe application particularly improved the most of biochemical properties in cv. Trakya Ilkeren.  相似文献   

9.
Jianguo Wu 《Landscape Ecology》2017,32(12):2225-2239
  相似文献   

10.
This study has been conducted with the aim to determine the type of nutrient medium that can be used in micropropagation studies for ‘Öküzgözü’ and ‘Bo?azkere’ and to specify BAP concentrations. In the study where ejectors with a length of 0.7–0.8?cm that are obtained with single-node culture are used, it was focused on four different nutrient media such as MS, DKW, QL and WPM and on six different concentrations such as 0.2–0.4–0.6–0.8–1.0–1.5 mg l?1 BAP. Single-node suspension explants which will be used in initiating the culture, are taken into culture in MS nutrient medium and the nutrient medium is supported with 30?g l?1 sucrose, 6?g l?1 agar and 1?mg l?1 BAP. In the trial environment, parameters such as number of shoots, shoot length (cm), number of nodes and callus ratio have been investigated. For both grape varieties, the best outcome was obtained with MS nutrient medium with respect to number of shoots, shoot length, and number of nodes. These values were found as 4.66, 1.24 and 6.39 for ‘Öküzgözü’ variety respectively, whereas they are determined as 6.28, 1.15 and 6.81 for ‘Bo?azkere’ variety respectively. In both grape varieties in DKW nutrient medium, starting from the 2nd week of culture, obscuration began to appear on the shoots and after this stage no other development has taken place.  相似文献   

11.
In order to study the effect of nitrogen spray at different times on color and yield parameters of grape cv. Pykany, an experiment was carried out in factorial based on randomized complete block design with four replications in Khalil Abad County in 2015. The treatments included three concentrations of nitrogen (0, 5 and 10?ppm) and three spray times (Late April, before bloom stage, and after pollination and fruit set stage). Color parameters such as brightness rate (L), redness (a) and yellowness (b), chroma, hue angle and browning index were evaluated in this experiment. The results of variance analysis and means comparison showed that the effect of treatment containing spray at concentration of 10?ppm on yield was significant at 1%. There were not significant differences among treatments of urea concentrations and spray times on L and b but different concentrations of urea had significant effect on a* at the level of 1%, so that the highest amount of significant difference was due to the treatment of urea spray at the concentration of 10?ppm and the lowest amount was due to the control treatment. Furthermore, the interaction effect of concentration and different times of nitrogen application on color intensity index was significant at the level of 5%. Based on the results, the effect of different concentrations of urea spray on hue angle was significant at 1% so that the highest level of significant difference was due to the concentration of 10?ppm and the lowest was related to control treatment.  相似文献   

12.
Yield management is important subject in modern grape growing and winter pruning and cluster thinning treatments were used for yield control. Standard cluster thinning is generally labor intensive and expensive method and grape growers try to new methods, which are alternative to cluster thinning for reducing berry set. The goal of the present research was to investigate the effects of chemical thinning treatments such as ethephon (ETH), gibberellic acid (GA3), naphthalene acetic acid (NAA) and non-chemical thinning treatments such as early leaf removal (ELR) and standard cluster thinning (SCT) on decreasing grape yield and enhancing berry composition of cv. Shiraz. In this study, percentages of berry set of cv. Shiraz were decreased by both chemical and non-chemical thinning treatments compared to Control. The results from these two different thinning methods shown certainly significant differences in berry composition of cv. Shiraz. Eventually, the best results in improved quality characteristics of cv. Shiraz were obtained from ELR treatment and treatments of ELR, ETH, GA3, NAA and SCT successively resulted in considerable enhancements in quality attributes of cv. Shiraz.  相似文献   

13.
This research was conducted to determine the changes in the seasonal carbohydrates of annual shoots and growth characteristics of 10 years old ‘Trakya ?lkeren’ grape variety. Grapevines are grafted on 5BB and 5C rootstocks and they were grown in heavy clay soil conditions. In the study, changes of sugar, starch and total carbohydrates were determined from bud burst to dormancy. While sugar, starch and total carbohydrates showed significant differences (P < 0.01) there were no differences between rootstocks. Although sugar, starch and total carbohydrates of shoots decreased from bud burst to blooming, they increased thereafter until vegetative growth stopped. Total carbohydrates of annual shoots have been found to accumulate in period from blooming to harvest. Total and mean leaf area, shoot length and diameter, total chlorophyll and chlorophyll a/b ratio showed significantly variation among rootstocks and phenological stages (P < 0.01). Whereas the highest chlorophyll contents were found in the blooming, it was found at the lowest through to harvest. Overall mean and total leaf area, shoot length and shoot diameter, total chlorophyll content and chlorophyll a/b ratio were significantly higher on 5BB grafted vines. In the study, 5BB rootstocks are found suitable in terms of shoot carbohydrate accumulation and growth characteristics for ‘Trakya ?lkeren’ grape variety.  相似文献   

14.
Sunlight influences many morphological, biochemical and molecular changes in fruits and the most important is color change associated with the development of pigments. The exposure to sunlight influences the anthocyanin content of the fruit peel. With the aim to understand the influence of bagging of fruits on the mango peel color, anthocyanin and expression of anthocyanin biosynthetic genes, an experiment was conducted by covering fruits of red colored cv. ‘Lily’ with muslin cloth. The observations were recorded for fruits of control (non-bagged) and fruits developing in shade. In bagged fruits, the color of peel changed towards lightness and yellowness. The reduction in anthocyanin was observed up to 17 times in bagged and shaded fruits. We also observed the down-regulation of all anthocyanin biosynthetic genes in fruits under bagged and shaded conditions. This study reports the influence of bagging and shade on anthocyanin biosynthetic genes and subsequent change in the color of fruit peel.  相似文献   

15.
AIM: To observe the effect of immune-activated platelets and low-density lipoprotein cholesterol (LDL) on the expression and activity of cyclooxygenase-2 (COX-2) and peroxisome proliferator activated receptor α (PPAR-α) in human umbilical vein endothelial cells (HUVECs) treated with activated platelets and LDL. METHODS: The platelets were activated by ADP. The co-culture system of HUVECs with immune activated platelets and/or LDL were established. The activity of COX-2 and expression of PPAR-α at mRNA and protein levels in HUVECs were detected by RT-PCR and Western blotting. The concentration of PGE2 was measured by ELISA for representing the COX-2 activity. The PPAR-α activity was determined by a nuclear factor assay kit. RESULTS: The COX-2 activity and mRNA expression of PPAR-α, the protein levels of COX-2 and PPAR-α and PGE2 concentration in activated platelets group were significant higher than those in un-activated platelets group (all P<0.01). No difference of PPAR-α binding activity was observed between two groups. LDL didnt affect the COX-2 activity and PPAR-α expression, but significantly promoted the stimulating effect of immune-activated platelets. CONCLUSION: Immune-activated platelets significantly promote COX-2 activity and PPAR-α expression in HUVECs, but dont change the PPAR-α binding activity. LDL at general concentration does not affect the expression and activity of COX-2 and PPAR-α, but promote the effect of activated platelets on HUVECs.  相似文献   

16.
AIM: To investigate the role of dual-specificity tyrosine phosporylation-regulated kinase 1A (Dyrk1A)-alternative splicing factor (ASF)-calcium/calmodulin-dependent protein kinase Ⅱδ (CaMK Ⅱδ) pathway in the progression of myocardial hypertrophy in renovascular hypertensive rats. METHODS: The renovascular hypertension was induced by two-kidney one-clip (2K1C) method. The changes of blood pressure and myocardial hypertrophy were measured. The techniques of RT-PCR and Western blotting were used to detect CaMKⅡδ alternative splicing and the protein expression of Dyrk1A and ASF, respectively. RESULTS: Eight weeks after operation, systolic blood pressure (SBP) and diastolic blood pressure (DBP) in 2K1C rats increased (P<0.05). The increases in left ventricular weight (LVW), the ratio of LVW to body weight (BW) and the area of myocardial cells indicated that the hypertensive rats developed significant cardiac hypertrophy. The protein expression of Dyrk1A and mRNA expression of CaMKⅡδA and δB were significantly increased, while the protein expression of ASF and mRNA expression of CaMKⅡδC were decreased compared with sham-operated control rats (P<0.05). Treatment with Dryk1A inhibitor epigallocatechin gallate (EGCG) or harmine effectively attenuated cardiac hypertrophy and reversed the changes in the protein expression of Dyrk1A, ASF and alternative splicing of CaMKⅡδ (all P<0.05). CONCLUSION: Dyrk1A-ASF-CaMKⅡδ pathway plays a role in the development of myocardial hypertrophy in renovascular hypertensive rats.  相似文献   

17.
This study was focused not only at the assessment of effects of different sewage sludge application rates on vegetative parameters and yield of raspberry (Rubus ideaus L.) but also heavy metal accumulation in the soil and raspberry leaves. A three-year field study was set up in a completely randomized block design with five sewage sludge application rates (0.0, 2.5, 5.0, 7.5 and 10.0?kg of dry matter per plant) and three replications. One-year-old, virus free ‘Heritage’ saplings were used for the experiment. The results of this study clearly indicated that the sewage sludge application is an effective mean for improvement of vegetative growth, yield, soil and plant chemical properties of raspberry in light textured soils. In addition to macro-element contents, sewage sludge application also caused significant changes in micro-element content of soils. No adverse effects of these increases were observed on plants throughout the experimental period. The most effective application rate was found as 7.5?kg per plant for this ecological condition. It can be concluded that when properly treated and applied to farmland sewage sludge is not only disposed economically but also improved vegetative growth and yield of raspberry.  相似文献   

18.
Foliar sprayings of grapevines with different plant growth regulators or chemicals were evaluated for determining grape skin color and phenolic compounds content and enhancing table grape quality characteristics of cv. Red Globe in this study. Grape skin color is one of the most important quality factors for table grapes. Sometimes, it can be viewed difficulties in skin coloration of some red table grape cultivars grown in various grape growing regions of the world and poor skin coloration of red table grapes is a frequent trouble that reduces production efficiency. Despite the fact that it is utilized from some canopy management practices; plant growth regulators, chemicals with plant hormones and hormone-like compounds as active ingredients can be recently used for improving anthocyanin accumulation in growing of some table grape cultivars. In current study, nine different preharvest treatments were used for improving quality parameters of cv. Red Globe, including 1?Control (C), 2–300?mg/L Abscisic acid (ABA), 3–300?mg/L Ethephon (Eth), 4–30% Ethanol (EtOH), 5–300?mg/L ABA + 300?mg/L Eth, 6–300?mg/L ABA + 30% EtOH, 7–300?mg/L Eth + 30% EtOH, 8–300?mg/L ABA + 300?mg/L Eth + 30% EtOH and 9?Cluster tip cutting (CTC). Although the best results from total phenolic compounds content and total anthocyanin content of cv. Red Globe were obtained in CTC treatment in all preharvest treatments, Eth+ETOH treatment especially gave rise to the best results among the preharvest chemical treatments.  相似文献   

19.
AIM: To study the effect of gossypol on the cognitive function of type 2 diabetic rats, and to explore its mechanism. METHODS: Thirty male Sprague-Dawley rats were divided into three groups randomly: normal group, type 2 diabetic group and gossypol treated group. After fed with high-fat diet for 4 weeks, the later two groups were injected with streptozotocin intraperitoneally to establish type 2 diabetic rat model. The animals in gossypol treated group were given gossypol at dosage of 15 mg/kg once per day for 4 weeks by gavage. Since 5th week, the times of gavages were changed into once per week at the same dosage and lasted to 12th week. Learning and memory abilities of rats were assayed with Morris water maze test. The concentration of blood glucose was measured by biochemical method. The levels of serum corticosterone and insulin were detected by enzyme-linked immunosorbent assay and radioimmunoassay, respectively. The protein expressions of 11β-HSD1 and GR in cerebral cortex and hippocampus were determined by Western blotting. The morphological changes of cerebral cortex and hippocampus were observed under light microscope and transmission electronic microscope, respectively. RESULTS: Compared to normal group, the karyopyknosis, dilation of golgiosome and mitochondria swelling of neuron from cerebral cortex and hippocampus were prominent in diabetic group. The concentrations of blood glucose, serum corticosterone and insulin increased significantly (P<0.01). Protein expression of GR decreased (P<0.05), 11β-HSD1 protein tended to increase. Platform searching score was lower (P<0.01) and escape latency was longer (P<0.01) in diabetic group. After treated with gossypol, the concentrations of blood glucose, serum corticosterone and insulin declined (P<0.01). The protein expression of 11β-HSD1 was decreased (P<0.05) and GR was increased (P<0.05). Escape latency was shorter (P<0.01) and platform searching score was increased (P<0.01). CONCLUSION: Gossypol may improve the cognitive function of type 2 diabetic rats. Decreasing the level of 11β-HSD1 and increasing GR protein in the brain may be involved in the mechanism.  相似文献   

20.
In this study, average weight, colour, soluble solids content and titratable acidity were determined as quality parameters of strawberry fruits while ascorbic acid, total anthocyanins, total phenolics, total flavonoids, minerals and trace elements content as nutritional parameters. All these attributes along with the DPPH radical scavenging activity were screened in two strawberry genotypes grown on the same site at the ripe stage. Also, the phenolic profiles of the strawberry fruits were obtained by HPLC. Except colour, results showed no significant differences between cultivars in quality parameters. Ascorbic acid found for ‘Premial’ cv. (81.62?mg/100?g) was twice the amount found in ‘Magic’ cv. (42.15?mg/100?g), total phenolics varied from 207.95 (cv. ‘Premial’) to 249.98 (cv. ‘Magic’) mg GAE/100?g while total flavonoid content in fruits of ‘Magic’ cultivar (79.56?mg/100?g) was 2?fold higher than the one of the ‘Premial’ cv. The quercetin content ranged from 2.72 to 4.66?mg/100?g, whereas free ellagic acid ranged from 6.79 to 9.03?mg/100?g. The pale shiny fruits of ‘Premial’ cv. (higher L*, ho and chroma index) had a lower total anthocyanin content (19.07?mg CG/100?g) than the bluish fruits of the ‘Magic’ cv. (23.96?mg CG/100?g). An inverse relationship between the levels of ascorbic acid and anthocyanin contents was also noticed in strawberry samples. Significant differences were found also in minerals and trace elements content of the two strawberry cultivars. The results revealed the importance of genetic background for the content of antioxidant compounds in strawberry fruits.  相似文献   

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