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1.
Fish fillet quality has been shown to be influenced by the level of antioxidants in preslaughter diet. Thus, an experiment was conducted to study the effect of different levels of vitamin E and C on the fillet quality of market‐size reared turbot (Scophthalmus maximus). Turbot of a mean initial weight of 347 ± 20 g were divided into four groups and fed commercial turbot diets (60% protein, 12% fat), supplemented with α‐tocopheryl acetate (mg kg?1) and ascorbyl‐2 monophosphate (mg kg?1) at the following dietary levels: 500/100, 1000/100, 100/1000, 100/100 respectively. Over a dietary supplementation period of 15 weeks, fish were fed to satiation and reached a final mean weight of 916 ± 29 g. α‐Tocopherol levels increased significantly (P < 0.001) in tissue (i.e. muscle, liver, heart and kidney) of fish fed diets containing elevated levels of α‐tocopheryl acetate. In ice storage, fillets of these fish exhibited significantly lower (P < 0.001) levels of lipid oxidation, and showed significantly less (P < 0.001) colour deterioration (higher hue angle and lower chroma). Elevated dietary α‐tocopheryl acetate levels had a negative effect (P ≤ 0.001) on the concentration of ascorbic acid in muscle tissue. An increase in dietary vitamin C did not have any detectable effect on fillet quality. Prolonged feeding times had a negative effect on lipid oxidation (P < 0.001) and colour deterioration (P < 0.01). These results suggest that increased dietary α‐tocopheryl acetate could prevent colour deterioration and lipid oxidation of turbot fillets in retail storage on ice.  相似文献   

2.
The present study investigated the distribution of α‐tocopherol (vitamin E) in fillets of turbot (Scophthalmus maximus) and Atlantic halibut (Hippoglossus hippoglossus). Turbot and Atlantic halibut were fed commercial diets, supplemented with different levels of α‐tocopheryl acetate at the dietary target levels of 100, 500 and 1000 mg α‐tocopheryl acetate kg?1 diet. The actual levels were 72, 547 and 969 for turbot, while halibut received 189, 613 and 875 mg α‐tocopheryl acetate kg?1 diet. Turbot were fed the diets for 24 weeks, while Atlantic halibut were fed for 20 weeks prior to slaughter. At the end of the feeding periods fish had reached a final weight of around 1 kg. Fish were slaughtered and filleted. From the four fillets obtained per fish, 22 samples were taken from designated areas and analysed for their α‐tocopherol content. The average concentrations of α‐tocopherol incorporated in turbot and Atlantic halibut increased with increasing levels of α‐tocopheryl acetate in the diet. Atlantic halibut had significantly (P < 0.05) more α‐tocopherol in positions 2/II and 1/I than in position 9/IX. Turbot had significantly (P < 0.05) more α‐tocopherol in position 2/II than in positions 1/I, 4/IV and 11/XI. By mapping α‐tocopherol concentrations in fish fillets, a high degree of quality prediction may be established. Moreover, this study may help scientists in their choice of sampling position, when investigating if α‐tocopheryl acetate supplementation resulted in successful α‐tocopherol incorporation.  相似文献   

3.
The effects of dietary α‐tocopheryl acetate supplementation and different slaughtering methods were investigated on the flesh quality of farmed market‐size turbot Scophthalmus maximus (L). Turbot were divided into three groups and fed commercial diets, supplemented with different levels of α‐tocopheryl acetate at the following dietary inclusion levels: 72 (100), 547 (500), 969 (1000) (mg of α‐tocopheryl acetate kg?1 diet, analytical values with diet codes in brackets). After 5 months, fish (mean weight 1056 ± 19.7 g) from each dietary treatment were sampled, applying three different slaughtering methods: (A) bleeding in ice water; (B) thermal shock, no bleeding; (C) percussion followed by bleeding in ice water. The time course of rigor mortis was evaluated, using pH, rigor index and mechanical compression tests. The results showed that the three parameters corresponded very well. Percussive stunning resulted in higher initial post‐mortem pH (P < 0.01) and a significantly delayed onset of rigor mortis (P < 0.05). Diet significantly affected shelf‐life, with fillets from fish fed diets 500 and 1000 having lower TBARS (thiobarbituric acid reactive substances) numbers from day 2 (P < 0.001) and less colour deterioration from day 7 of storage on ice onwards (P < 0.05). These results suggest that an increase in dietary α‐tocopheryl acetate before slaughter as well as careful selection of the slaughtering method may greatly enhance the flesh quality of market‐size turbot.  相似文献   

4.
To investigate the potential synergistic effects of dietary ascorbic acid (AA), α‐tocopheryl acetate (TA) and selenium (Se) supplementation above minimum requirement levels on the growth performance and disease challenge of fingerling Nile tilapia, Oreochromis niloticus L., five experimental diets were formulated: control (150 mg AA, 100 mg TA and 0.2 mg Se per kg diet), excessive ascorbic acid (eAA) (2000 mg AA, 100 mg TA and 0.2 mg Se per kg diet), excessive α‐tocopheryl acetate (eTA) (150 mg AA, 240 mg TA and 0.2 mg Se per kg diet), excessive selenium (eSe) (150 mg AA, 100 mg TA and 0.5 mg Se per kg diet) and excessive all (eALL) (2000 mg AA, 240 mg TA and 0.5 mg Se per kg diet). Experimental fish averaging 2.9 were randomly distributed in each aquarium as a group of 40 fish with total weight 116±2.9 g (mean±SD). Each diet was fed on a dry‐matter basis to fish in three randomly selected aquaria at a rate of 4–8% of total body weight daily. After 10 weeks of the feeding trial, fish fed eAA, eTA and eALL diets had significantly higher weight gain, feed efficiency ratio, protein efficiency ratio and specific growth rate than fish fed eSe and control diets (P<0.05). There was no significant difference among fish fed five experimental diets in cumulative mortalities when fish were challenged with Edwardsiella tarda at the end of the experimental period. These results indicate that sufficient supplementation of dietary AA or TA had positive effects on growth performance, but there was no synergistic effect of excessive dietary AA, TA and Se supplementation on growth performance and disease resistance to E. tarda in fingerling Nile tilapia.  相似文献   

5.
A feeding experiment was conducted to investigate the effects of high dietary intake of vitamin E (supplied as dl ‐α‐tocopheryl acetate) and n‐3 highly unsaturated fatty acid (n‐3 HUFA) on the non‐specific immune response and disease resistance in Japanese flounder Paralichthys olivaceus. Nine practical diets were formulated to contain one of three levels of vitamin E namely, 0, 80 or 200 mg kg?1 (the total α‐tocopherol contents in the diets were 21, 97 and 213 mg kg?1 based on analysis), and at each vitamin E level with one of three n‐3 HUFA levels i.e. 0.5%, 1.5% or 2.0%. Each diet was randomly assigned to triplicate groups of Japanese flounder (initial body weight: 40.5±1.0 g, mean±SD) in a re‐circulation rearing system. Fish were fed twice daily to apparent satiation at 07:00 and 18:00 hours for 12 weeks. During the experimental period, water temperature was maintained at 18±1°C, salinity 31–35 g L?1, and pH 7.8–8.2. Dissolved oxygen was not less than 6 mg L?1, and there were negligible levels of free ammonia and nitrite. The results showed that the increase in dietary n‐3 HUFA from 0.5% to 1.0% significantly decreased muscle α‐tocopherol contents in fish‐fed diets with 21 and 97 mg α‐tocopherol kg?1 diet (P<0.05). In 1.0% HUFA groups, alternative complement pathway activity (ACH50) of fish fed the diet containing the 213 mg α‐tocopherol kg?1 diet was significantly higher than noted for fish fed the diet containing 97 mg α‐tocopherol kg?1 diet (P<0.05). Fish fed the diet with 213 mg α‐tocopherol kg?1 and 2.0% n‐3 HUFA had the highest lysozyme activity (131.7 U mL?1) among all the dietary treatments. Fish fed the diets containing 97 and 213 mg α‐tocopherol kg?1 with 1.0% n‐3 HUFA had significantly higher respiratory burst activity than those fed the diets containing 21 mg α‐tocopherol kg?1 with 0.5 and 1.0% n‐3 HUFA (P<0.05). In the disease resistance experiment, high intake of dietary vitamin E with 213 mg α‐tocopherol kg?1 significantly decreased cumulative mortality and delayed the days to first mortality after a 7‐day Edwardsiella tarda challenge (P<0.05). In addition, under the experimental conditions, dietary vitamin E and n‐3 HUFA had a synergistic effect on the non‐specific immune responses and disease resistance in Japanese flounder (P<0.05).  相似文献   

6.
This study was conducted to evaluate the dietary α‐tocopherol (vitamin E) requirement in juvenile sea cucumber, Apostichopus japonicus. Sea cucumbers averaging 1.48 ± 0.07 g (mean ± SD) were randomly distributed into 18 rectangular plastic tanks of 20 L capacity in a recirculating system (20 animals per tank). Six semi‐purified experimental diets with average protein and crude lipid levels (dry matter) of 29.7 ± 0.36% and 4.39 ± 0.23% (mean ± SD), respectively were formulated to contain 0 (E4), 15 (E12), 30 (E23), 60 (E44), 120 (E77) and 600 (E378) mg α‐tocopherol/kg diet, supplied as dl‐α‐tocopheryl acetate. Diets were analyzed for α‐tocopherol content by HPLC and the α‐tocopherol levels were 4.01, 12.4, 23.1, 44.3, 77.4 and 378 mg α‐tocopherol/kg diet for E4, E12, E23, E44, E77 and E378 diets, respectively. Casein and defatted fish meal were used as the protein sources in the diets while wheat flour was the carbohydrate source. Sea cucumbers were fed each of the six experimental diets in triplicate groups. At the end of the 14‐week feeding trial, weight gain (WG), specific growth rate (SGR) and feed efficiency (FE) of sea cucumbers fed on E23, E44, E77 and E378 diets were significantly (P < 0.05) higher than those of animals fed on E4 and E12 diets. However, there were no significant differences in WG, SGR and FE among sea cucumbers fed on E23, E44, E77 and E378 diets or among those fed on E4 and E12 diets. Survival of sea cucumbers fed on E44, E77 and E378 diets were significantly higher than those of animals fed on E4, E12 and E23 diets. However, there were no significant differences among sea cucumbers fed on E4, E12 and E23 diets or among those fed on E44 and E77 diets. Whole‐body vitamin E concentration increased with α‐tocopherol content of the diets. Broken line analysis of WG showed an optimum dietary α‐tocopherol requirement of 41 mg α‐tocopherol/kg diet in sea cucumber. These results indicated that the optimum dietary α‐tocopherol requirement in sea cucumber in the form of dl‐α‐tocopheryl acetate could be higher than 23.1 mg α‐tocopherol/kg diet but lower than 44 mg α‐tocopherol/kg diet.  相似文献   

7.
This study was conducted to determine the effects of increasing dietary lipid concentrations on fillet characteristics of post-juvenile rainbow trout. A feeding trial was conducted with fish meal based diets containing 10, 15, 20, 25, or 30% lipid for 24 wk. Menhaden oil was the lipid ingredient. Weight gain was significantly greater in fish fed the 30% lipid diet than in fish fed either 10%, 15% or 20% lipid diets. There were no significant differences in visceral somatic index. Fillet lipid concentration of fish fed the 30% lipid diet (9.2-g lipid/ 100-g fillet) was significantly higher (P < 0.05) than fish fed either the 10% or 15% lipid diets (5.8- and 6.9-g lipid/100-g fillet, respectively). In samples stored for 3 d at 5 C or S wk at -20 C, sensory panelists reported that the cooked fillets from fish fed the 30% lipid diet were "more fishy" than fish fed the 15% lipid diet, and preferred the cooked fillets from the 15% lipid treatment over the 30% lipid treatment. Triangle tests and fillet colorimetry showed no significant differences between fillets from fish fed the 15% and 30% lipid diets at any sampling time point. No significant differences in fillet concentrations of thiobarbituric reactive substances were observed among dietary treatments stored at either 5 C or -20 C. These results suggest that two main effects of feeding a 30% lipid diet (with fish oil as the lipid source) are a higher lipid concentration in the fillet and a "fishier" aroma compared to fillets from fish fed a 15% lipid diet.  相似文献   

8.
The impact of dietary α‐tocopherol on juvenile Chinese mitten crab Eriocheir sinensis was experimentally evaluated in a 10‐week study. Crab were fed with nine diets including three levels of α‐tocopherol (0, 100 and 300 mg kg?1 diet) and three levels of fish oil oxidation (fresh, moderate and high) in triplicates. Fresh and moderate oil oxidization enhanced weight gain, but moderate and high oil oxidization lowered survival and feed efficiency. The 100‐mg α‐tocopherol kg?1 diet resulted in higher hepatopancreas MDA than other α‐tocopherol diets. High oil oxidization led to the lowest serum superoxide dismutase (SOD) and glutathione peroxidase (GPH‐PX). The serum SOD and GPH‐PX activities in crab fed 100 mg α‐tocopherol were higher than in those fed other α‐tocopherol diets. The diet without α‐tocopherol addition lowered lysozyme and phenoloxidase (PO) activities compared to other α‐tocopherol diets. Fresh fish oil diet increased PO activity compared to oxidized oils. High oil oxidization caused significantly more mortality than fresh or moderate oxidization after 7‐d postchallenge with Aeromonas hydrophila. Supplementation with α‐tocopherol significantly enhanced resistance to bacterial infection. This study indicates that α‐tocopherol can protect lipid from peroxidation and enhance disease resistance.  相似文献   

9.
A 15‐wk study was conducted to evaluate the effect of supplemental menhaden fish oil levels and feeding duration on growth performance and tissue proximate and fatty acid (FA) compositions of juvenile channel catfish, Ictalurus punctatus. Dietary fish oil levels had no effect on final weight gain, feed efficiency, and survival of channel catfish. Tissue lipid contents were directly correlated to dietary lipid levels, while moisture contents were inversely related to dietary lipid levels. Fillet moisture contents progressively decreased, whereas fillet lipid increased with increasing feeding duration. Significant increase in saturated and total n‐3 FAs and decrease in monoenoic and total n‐6 FA in whole body and fillet were observed at each incremental level of dietary fish oil. Percentages of n‐3 and n‐3 highly unsaturated fatty acids in fillet of fish fed the control and 3% fish oil diets decreased with increasing feeding periods, whereas those of fish fed 6 or 9% added fish oil diets remained stable or increased. Ratios of n‐3/n‐6 were statistically comparable throughout the 15‐wk feeding. When expressed in terms of mg/g of fillet, the highest concentration of n‐3 was obtained in fillets of fish fed the 9% added fish oil diet for 15 wk.  相似文献   

10.
Turbot and Atlantic halibut are highly valued fish species. However,very little is known about fillet shelf-life characteristics associated withboth species. Thus, fillet -tocopherol content and proximate compositionof wild turbot (1.5 kg) and Atlantic halibut (1.1 kg)caught off the south coast of Ireland and the north-west coast of Iceland,respectively, were investigated. In addition, the susceptibility of fillets, storedunder retail conditions, to lipid oxidation and colour change was studied.Proximate composition analysis showed that turbot had significantly highermoisture (P < 0.001) and lower protein (P < 0.001) contents compared toAtlantic halibut. Atlantic halibut incorporated significantly higher (P <0.001) levels of -tocopherol into fillets than turbot. Over 14 days ofstorage on ice, fillets from Atlantic halibut exhibited significantly lower (P =0.020) levels of lipid oxidation than those of turbot. However, malondialdehyde(MDA) concentrations were generally very low, never exceeding 0.6 gg–1 fillet. Turbot maintained a significantly higher (P< 0.001) pH over the storage period. The lightness (L* values) offillets from both species increased over 14 days of storage, but wassignificantly higher (P < 0.001) in Atlantic halibut than in turbot. Turbotdeveloped a relatively intense yellow colour during storage (decrease in hueangle and increase in b* values), whereas this was not the case forAtlantic halibut. The results of this study demonstrate that fillets of wildAtlantic halibut stored on ice, were less prone to lipid oxidation anddiscolouration than those of wild turbot. However, quality changes in turbotwere very small showing that both fish have tremendous shelf-life capacities interms of lipid oxidation. These findings are considered in the context of knownmaterial for farmed fish.  相似文献   

11.
A study was conducted to evaluate low‐protein traditional or alternative diets for pond‐raised hybrid catfish, Ictalurus punctatus × Ictalurus furcatus. Three 24% protein diets containing decreasing levels of soybean meal (30, 20, and 15%) and increasing levels of cottonseed meal and corn germ meal were compared with a 28% protein control diet. Hybrid catfish fingerlings (mean initial weight = 71 g/fish) were stocked into 20 earthen ponds (0.04 ha) at a density of 14,826 fish/ha with five ponds per dietary treatment. Fish were fed once daily to apparent satiation for a 191‐d growing season. There were no significant differences in total diet fed, net yield, weight gain, feed conversion ratio (FCR), survival, or fillet proximate nutrient composition among dietary treatments (P ≥ 0.10). However, regression analysis showed for fish fed 24% protein diets there was a linear increase in FCR as soybean meal levels decreased (P = 0.06). Compared with fish fed the 28% protein control diet, fish fed 24% protein diets had lower carcass and fillet yield. Results demonstrate a 24% protein alternative diet containing 20% soybean meal may be substituted for 28% protein diets for hybrid catfish during food fish production.  相似文献   

12.
Rainbow trout (186 g) were fed three test diets where the lipid source (150 g kg?1) was either menhaden oil (MO), pollock oil (PO) or canola oil (CO) for eight weeks to an average weight of 370 g. The CO group was then divided into two groups, one continuing on the CO diet and the other switched to the PO diet (CO–PO). After nine additional weeks of feeding, the average fish weight approximately doubled (719–749 g). No significant differences were found in average final weight or fillet yield among dietary treatment groups. Fatty acid profiles of fillets from trout fed MO, PO or CO‐supplemented diets reflected the fatty acid profiles of the added oils, whereas the fatty acid profile of fillet from trout in the CO–PO group exhibited values similar to those of fish fed PO. The ratio of ω3 : ω6 FA was nearly 2.5 times higher in fillets from the CO–PO group compared to the CO group. Sensory analysis showed that panelists preferred CO‐fed fillets over those fed MO, PO, or CO–PO. Phase‐feeding CO and PO reduced fish oil use and resulted in fillets with double the content of Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA) over CO‐fed fish, similar to levels in MO‐fed fish.  相似文献   

13.
Channel catfish, Ictalurus punctatus, 88.4 ± 2.6 g/fish, were fed a basal diet amended with 4% of three processed menhaden, Brevoortia tyrannus, oils. These were compared with basal diets amended with 4% corn oil or 4% canola oil. Three replicate aquaria of nine fish each were fed assigned diets twice daily. At 6 wk, fish were group weighed, fillets were collected for sensory evaluation, fatty acid analysis by gas chromatography (GC). In a second study, catfish, 118.8 ± 3.2 g/fish, were stocked into fifteen 0.04‐ha earthen ponds and fed once daily for 16 wk one of four diets containing 2 or 4% of either catfish offal oil or refined (RF) menhaden oil. At harvest, fillets were saved for sensory evaluation and fatty acid analysis. Results showed no significant (P > 0.05) differences among treatments for aquarium study and pond study variables such as weight gain, fillet proximate analysis, or pond production. GC analysis showed that levels of omega‐3 (n‐3) highly unsaturated fatty acids (HUFA) in fillet lipid were significantly (P < 0.05) elevated for fish fed menhaden oil diets. Sensory evaluation revealed that fillets from fish fed RF menhaden oil had satisfactory flavor and could be a source of n‐3 HUFA for humans.  相似文献   

14.
An experiment was conducted to determine the effects of different levels of dietary vitamin C (VC) and E (VE) supplementation on fillet quality of red sea bream fed oxidized fish oil (OFO). Fish with an average body weight of 205.0 g were fed four test diets for 9 weeks. Control diet contained fresh fish oil (FFO) with 100 mg kg?1 of VE and 500 mg kg?1 of VC (FFO100E/500C). The other three diets contained OFO with varying levels of VE (mg kg?1) and VC (mg kg?1) (OFO100E/500C, OFO200E/500C and OFO200E/1000C). After feeding trial, two fillets from each fish by hand filleting were stored in a refrigerator at 4°C for 96 h during analyses. Results showed that fish fed OFO increased fillet thiobarbituric acid reactive substances (TBARS) and K‐value, and decreased fillet VC and VE concentrations during storage time. Supplementation of VC did not have any detectable effect on fillet quality. Increasing dietary VE supplementation increased fillet VE concentrations, reduced fillet TBARS and K‐value values of red sea bream. Therefore, we suggest that dietary supplementation of 200 mg kg?1 of vitamin E could improve fillet oxidative stability of red sea bream fed OFO.  相似文献   

15.
Economical, nutritious diets for hybrid striped bass (HSTB) are required for the continued expansion and sustainability of this industry. Turkey meal (TM) is a by‐product of the US turkey industry and is a potentially‐valuable local, alternative protein source for use in aquaculture diets because of its excellent nutritional composition and quality. TM may substitute for more expensive fish meal (FM)‐based diets; however, there are no published data with regard to using this ingredient in sunshine bass diets. Therefore, a 16‐week feeding trial was conducted with juvenile (36 g) sunshine bass (Morone chrysops × Morone saxatilis) to evaluate growth, feed conversion and body composition when fed diets with decreasing levels of FM (300, 200, 100 and 0 g kg?1) and increasing levels of turkey meal (0, 97, 175 and 264 g kg?1). Four practical diets were formulated to contain 400 g kg?1 protein and similar energy levels. Twenty fish were stocked into each of the 12, 1200‐L circular tanks and were fed twice daily ad libitum. At the conclusion of the feeding trial, there were no significant (P > 0.05) differences in final mean weight, percentage weight gain, specific growth rate and feed conversion ratio among treatments, which averaged 363.7 g, 904.3%, 2.02% day?1 and 1.73, respectively. Percentage survival of fish fed diet 4 (0 g kg?1 FM and 264 g kg?1 TM) was significantly (P > 0.05) lower (survival = 88.3%) than fish fed diet 3 (100 g kg?1 FM and 175 g kg?1 TM; survival = 95%), but not different from fish fed diet 1 (survival = 92.5%) and fish fed diet 2 (survival = 93.3%). Fillet weight and amount of abdominal fat were not significantly different among all treatments and averaged 258 and 58 g kg?1, respectively. Fish fed diet 1 (300 g kg?1 FM, 0 g kg?1 TM) and diet 2 (200 g kg?1 FM and 970 g kg?1 TM) had a significantly (P < 0.05) lower hepatosomatic index (2.83 and 3.01, respectively) than fish fed diet 4 (3.33), but not different (P > 0.05) compared to fish fed diet 3 (3.14). Lipid in the fillet of fish fed diet 2 (197 g kg?1) was significantly (P < 0.05) higher than fish fed all other diets; and the percentage lipid in the fillet of fish fed diet 1 (126 g kg?1) was significantly lower than fish fed diets 2 and 4, but not different (P >0.05) compared to fish fed diet 3. Fillet moisture, protein and ash were similar among fish fed all diets and averaged 748, 798 g kg?1 and 51.0 g kg?1 (dry‐matter basis), respectively. The amino acid composition of fillets was similar among all treatments with a few slight significant differences. Results from the present study indicate that tank‐grown sunshine bass can be fed a diet containing 264 g kg?1 TM with 0 g kg?1 FM, compared to diets containing up to 300 g kg?1 FM, without adverse effects on weight gain, growth rate, feed conversion and body composition. Further research should be conducted using lower‐protein diets to determine minimum protein level for tank‐grown sunshine bass.  相似文献   

16.
The aim of this work is to investigate olive oil by‐product [vegetation water (VW)] inclusion in rainbow trout diet and its effect on the productive traits and the quality of the product. Two levels of VW inclusion were used and one control group was included. Fish diets were isonitrogeonous (crude protein 40%) and isoenergetic (18 MJ kg?1 DM). Two thousand and four hundred rainbow trout were used. An in vivo digestibility experiment was performed in order to determine diets’ digestibility. All the fish diets and fillets were analysed to determine the proximate and fatty acid composition. On final fish fillet, lipid oxidation was determined at 0, 24, 48, 72, 96 and 192 h of storage using the 2‐thiobarbituric acid method (thiobarbituric acid reactive substances). Aroma analyses on the final cooked and raw fillet were performed using an electronic nose. The VW inclusion partially reduced protein digestibility. The fish growth varied between 1.08% and 1.1% day. The supplemental level of VW led to a better antioxidant status of fish fillet, in particular, in the fillet sample after 72 h of fillet conservation. Principal component analysis in raw and cooked fish fillet indicates that the VW inclusion in the fish diet led to aroma modification on fish fillet.  相似文献   

17.
18.
An 8‐week feeding trial was conducted to establish the dietary vitamin E requirement of juvenile cobia. The basal diet was supplemented with 10, 20, 30, 40, 60, 120 mg vitamin E kg?1 as all‐rac‐α‐tocopheryl acetate. The results indicated that fish fed the diets supplemented vitamin E had significantly higher specific growth rate, protein efficiency ratio, feed efficiency and survival rate than those fed the basal diet. It was further observed that vitamin E concentrations in liver increased significantly when the dietary vitamin E level increased from 13.2 to 124 mg kg?1. Fish fed the basal diet had significantly higher thiobarbituric acid‐reactive substances concentrations in liver than those fed the diets supplemented vitamin E. Fish fed the diets supplemented with 45.7 and 61.2 mg kg?1 vitamin E had significantly higher red blood cell and haemoglobin than those fed the basal diet, while fish fed the diets supplemented with 61.2 and 124 mg kg?1 vitamin E had higher immunoglobulin concentration than those fish fed the basal diet. Lysozyme and superoxide dismutase were significantly influenced by the dietary vitamin E level. The dietary vitamin E requirement of juvenile cobia was established based on second‐order polynomial regression of weight gain and lysozyme to be 78 or 111 mg all‐rac‐α‐tocopheryl acetate kg?1 diet, respectively.  相似文献   

19.
The influence of α-tocopheryl acetate (α-TOAc) on plasma concentration and fillet deposition of dietary astaxanthin was investigated in Atlantic salmon Salmo salar L. The diets were added 30 or 50 mg kg–1 astaxanthin, and 200, 400 or 800 mg kg–1α-TOAc at each astaxanthin level. Improved flesh deposition of astaxanthin by 8–14% was achieved for fish fed diets with 30 and 50 mg kg–1 astaxanthin, respectively, by the dietary addition of 800 compared with 200 mg kg–1α-TOAc. These results were supported by CIE[1976]L*a*b* tristimulus redness measurements (a* value). Plasma astaxanthin concentration mirrored the muscle astaxanthin concentration in the groups of fish fed a diet containing 30 mg kg–1 astaxanthin. The salmon fed a high astaxanthin and low α-TOAc diet had the highest plasma concentration of idoxanthin (P < 0.05). Astaxanthin retention was significantly higher (P < 0.001) in salmon fed 30 mg kg–1 astaxanthin than in those fed 50 mg kg–1 astaxanthin, but was not significantly affected by dietary α-TOAc. Liver weight, body weight, specific growth rate, feed/gain ratio and mortalities were not affected by dietary α-TOAc levels. In conclusion, the dietary addition of α-TOAc appears to increase astaxanthin fillet deposition in salmonids and may reduce the demand for astaxanthin supplementation. The effect was rather small and requires verification.  相似文献   

20.
Three isonitrogenous (520 g protein kg?1 DM) and isoenergetic (25 MJ kg?1 DM) diets containing increasing levels of flaxseed oil (FxO; 0%, 40% and 70% of total added oil) at the expense of fish oil (FO) were tested for 33 weeks in groups of 61 individually PIT‐tagged halibut (initial weight, 849 ± 99 g). Effects on fish growth performance, fillet nutritional and sensory quality were determined. Specific growth rate (0.2% day?1), feed conversion ratio (1.2–1.3) and nitrogen and energy retention were not affected by dietary treatments. Dietary fatty acid composition was reflected in fatty acid profiles of halibut muscle, liver and heart. Muscle of fish fed FxO diets contained higher 18:2n‐6 and 18:3n‐3 concentrations whereas 20:5n‐3 and 22:6n‐3 levels were significantly reduced. However, increasing FO replacement induced preferential retention of 22:6n‐3 especially in heart, and a trend for 20:5n‐3 conservation in heart and muscle was observed. FO replacement did not affect colour, texture and the characteristic fish odour and flavour of cooked fillets. By selectively retaining long‐chain polyunsaturated fatty acids halibut can adapt to a lower dietary supply without adverse effects on growth, feed conversion ratio, survival, and fillet nutritional and sensory quality.  相似文献   

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