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1.
培养时间对黑木耳单核菌株同32酶酶谱的影响 总被引：1，自引：0，他引：1
采用聚丙烯酰胺凝胶电泳分析了不同培养时间对黑木耳(Auricularia auricula)单核菌株7种酶同工酶的影响,其中EST、MDH、FDH的同工酶酶谱在菌丝生长阶段较稳定,培养20d的单核菌株H2和J3的EST、MDH和FDH的同工酶分别出现3个、2个和1个多态性位点,可用于H2与J3杂交后代同工酶基因位点的遗传分析.培养时间不同的H2和J3的ADH、GDH、ME、ACP的同工酶酶谱变化较小,单核菌株H2和J3的同工酶酶谱无显著的多态性表现. 相似文献
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采用聚丙烯酰胺凝胶电泳分析了不同培养时间对黑木耳（Auricularia auricula)单核菌种7种酶同工酶的影响，其中EST，MDH，FDH的同工酶酶谱在菌丝生长阶段较稳定，培养20d的单核菌株H2和J3的EST，MDH和FDH的同工酶分别出现了3个，2个和1个2多态性位点，可用于H2与J3杂交后代同工酶基因位点的遗传分析。培养时间不同的H2和J3的ADH，GDH，ME，ACP的同工酶酶谱变化较小，单核菌株H2和J3的同工酶酶谱无显著的多态性表现。 相似文献
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从云南、四川两省9个采样地共收集31个暗褐网柄牛肝菌(Phlebopus portentosus)子实体,通过组织分离获得菌株,分别利用固体、液体培养基培养菌丝体,对其进行酯酶同工酶分析,通过酶谱聚类分析研究分离菌株的遗传多样性。结果表明,固体培养的菌丝体共检出10个酯酶同工酶条带、21种酶谱类型,每个菌株具有2条~5条同工酶条带;液体培养菌丝体共检出11个同工酶条带、23种酶谱类型,每个菌株具有2条~6条同工酶条带;2种培养方式下所有菌株均检出一条相对迁移率R_f=0.13、相对分子质量为136 kDa的同工酶条带;不同培养方式相同菌株的酯酶同工酶酶谱存在较大差异。在遗传距离为0.220时,固体培养菌丝体的酯酶同工酶酶谱聚为9类,液体培养的聚为7类;在遗传距离为0.300时,2种培养方式的酶谱均聚为4类;聚类结果与地理来源无相关性。暗褐网柄牛肝菌具有丰富的遗传多样性,为后续种质资源保护、优良菌株筛选和种性鉴定等工作提供参考。 相似文献
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桦褐孔菌菌株过氧化物酶同工酶酶谱及其模糊聚类分析 总被引：2，自引：1，他引：1
本试验采用聚丙烯酰胺凝胶电泳技术,对采自6个不同地区的桦褐孔菌菌核分离得到的纯菌丝的过氧化物酶同工酶进行研究。结果表明,所有菌株酶带数目在2～8条,在Rf=0.75、Rf=0.98处所有菌株的酶带都较宽且着色较深,此为桦褐孔菌菌株的特征酶带。进一步采用Fuzzy聚类法进行数量分析表明,菌株间隶属度在[0.250,1.000],当隶属度为0.330时将8个菌株分类。结果显示,同一培养时期的各菌株间同工酶酶谱存在一定的种内及地理分布差异,部分菌株间存在较近的亲缘关系。 相似文献
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利用EST同工酶鉴定天麻品种及亲缘关系的研究 总被引：4，自引：0，他引：4
目的：鉴定3种天麻品种及其亲缘关系。方法：采用聚丙烯酰胺凝胶电泳(PAGE)对三种天麻菌株酯酶(EST)同工酶进行研究。结果：天麻不同菌株之间酯酶同工酶活性不同，酶谱条带数在4～5条，且具有特征谱带。B、G的酶谱条带数基本相同；R、B、G的酶谱条数依次分别为4条，5条，4条；其中基本酶带有3条；G号菌株与B号菌株的相似度指数为0．89，说明这两个菌株间亲缘关系最近。G号菌株与R菌株的相似度指数为0．75，反映它们之间的亲缘关系相对较远。 相似文献
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双孢蘑菇不同菌株生物学特性的研究 总被引：1，自引：0，他引：1
比较研究了13株双孢蘑菇菌株的菌丝生长势、出菇性状、菌丝拮抗性、酯酶同工酶酶谱等。结果表明,13个菌株中表现出一定的遗传差异性,部分菌株为同物异名。Ag-004菌株菌丝生长势最强,日平均生长速度0.54 cm·d~(-1),23d长满试管;出菇试验表明棕色蘑菇品种表现出比白色蘑菇品种产量高、较耐低温和抗逆性强的特性。大多数菌株之间表现出一定的拮抗反应,少部分菌株间拮抗现象不明显。酯酶同工酶酶谱出现3个区域,Rf=0.1758、0.2424、0.7697为所有菌株共有,可能为双孢蘑菇酯酶同工酶的特征谱带,Rf=0.8060为白色蘑菇品种的酯酶同工酶的特征条带,而Rf=0.7151为2个棕色蘑菇菌株所有,在所有的白色品种中几乎没有,Rf=0.7151可能为棕色蘑菇菌株的特征谱带。 相似文献
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杏鲍菇不同菌株生物学特性的研究 总被引：6，自引：2，他引：6
比较研究了杏鲍菇4个菌株的菌丝拮抗性、酯酶同工酶酶谱、菌丝生长速度、菌丝体生物量及多糖含量。结果表明，4个菌株问存在遗传差异。菌株X3菌丝生长速度最快，日均长速为0．69cm；菌株X2菌丝体生物量最高，为1．572g／100mL；菌株X1菌丝体多糖含量最低，仅为39.07μg／mg，菌株X2、X4和X3菌丝体多糖含量分别为84．28μg/mg、79．20μg／mg和78．99μg/mg。 相似文献
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粤北野生灵芝与栽培灵芝同工酶及可溶性蛋白的研究 总被引：1，自引：0，他引：1
采用垂直聚丙烯酰胺凝胶电泳技术,分别对粤北5株野生灵芝与9株栽培灵芝进行酯酶和过氧化物酶同工酶及可溶性蛋白进行比较与分析,并通过聚类分析对比各菌株间的遗传差异性.结果表明:酯酶和过氧化物酶同工酶酶谱在酶带数量和酶活性方面都表现出一定的差异;供试各菌株间可溶性蛋白差别不大.进一步采用两种同工酶综合聚类分析发现,14株菌株分为13类,野生灵芝菌株与栽培菌株之间差异较大;这也表明同工酶技术在灵芝分类、鉴定、和遗传标记等方面都具有参考作用,且粤北地区野生灵芝资源潜在较大的开发利用价值. 相似文献
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AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β-particles emission from 188Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by 188Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [3H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of 188Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from 188 Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G0/G1 arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation. 相似文献
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AIM:To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS:After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly (P＜0.01). The ET content in plasma also decreased significantly by L-Arg(P＜0.01).CONCLUSION: Plasma ET content and the expression of c-fos in the left ventricle of rats with renovascular hypertensive hypertrophy could be decreased by L-Arg administration. 相似文献
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《International Journal of Fruit Science》2013,13(3):101-120
Abstract Saskatoon berry (Amelanchier alnifolia Nutt., Rosaceae) and blueberry (Vaccinium corymbosum L., Ericaceae) are substantially equivalent in all characteristics that are important to the consumer, including fruit color, shape, size, nutrition, texture, and uses. In addition, both fruits are native to North America and they have practically identical historical uses and known health benefits. Their composition, processing, nutritional value and metabolism, intended uses, and levels of undesirable substances are compared. 相似文献
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多效唑对猕猴桃离体试管苗生长及内源激素的影响 总被引：18，自引：0，他引：18
多效唑（ＰＰ３３３）处理猕猴桃试管苗，降低了其生长强度；植株体内的ＧＡ３、ＩＡＡ和ＺＴ含量下降，ＡＢＡ的含量上升，乙烯释放率增加；并且能降低外源的ＧＡ３和ＩＡＡ促进生长的作用，而外源的ＧＡ３和ＩＡＡ又能不同程度地逆转多效唑的抑制作用，使植株恢复生长。 相似文献
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HU Zhi-cheng ZHU Jia-yuan ZHU Bin GUO Dong CHEN Bin ZHANG Kai HU Kun-hua LI Ming-tao TANG Bing 《园艺学报》2011,27(9):1802-1806
AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two-dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.RESULTS: A two-dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially-expressed proteins in keloid and hypertrophic scar identified from the spots of over 4-fold change, including 16 proteins in both keloid and hypertrophic scar (8 up-regulated and 8 down-regulated), 11 only in keloid (9 up-regulated and 2 down-regulated) and 9 only in hypertrophic scar (4 up-regulated and 5 down-regulated).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars. 相似文献
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The objective of this study was to establish a cryopreservation protocol for hawthorn shoot apices (Crataegus pinnatifida Bge.). Cryopreservation was carried out via encapsulation–dehydration, vitrification, and encapsulation–vitrification on shoot apices excised from in vitro cultures. We began by showing that cold-acclimation enhanced the regrowth of cryopreserved apices from 10.0 to 65.5% in encapsulation–dehydration. We then decided that the encapsulation–dehydration method was an optimal cryopreservation method for hawthorn shoot apices in terms of its high recovery after cryopreservation as well as its ease of use compared with vitrification and encapsulation–vitrification. In encapsulation–dehydration, the protocol leading to optimal regrowth was as follows: after cold-acclimation at 5 °C in the dark for 2 weeks, excised shoot tips were pretreated for 24 h at 25 °C on hormone-free Murashige and Skoog [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497] (MS) basal medium with 0.4 mol/L sucrose, then encapsulated and precultured in liquid MS medium with 0.8 mol/L sucrose for 16 h at 25 °C. Precultured beads were dehydrated for 6 h at 25 °C in the dessicator containing 50 g silica gel to a moisture content of 15.3% (fresh-weight basis) before cryostorage for 1 h. In addition, we examined the effect of adding glycerol to both the alginate beads and loading solution to enhance regrowth after cryopreservation in encapsulation–dehydration. In the present study, it was shown that adding 0.5 mol/L glycerol resulted in high regrowth percentages (82.5–90.0%) in four Crataegus species. 相似文献
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AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [3-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10-6-10-4 mmol/L) stimulated [3-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [3-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P＜0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P＜0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10-6-10-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P＜0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl2 for 6 h induced an increase cellular MT content by 5.7-fold (P＜0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P＜0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation. 相似文献
18.
Duncan Brean W. Boyle Shannon Breininger David R. Schmalzer Paul A. 《Landscape Ecology》1999,14(3):291-309
Historic landcover dynamics in a scrubby flatwoods (Tel-4) and scrub landscape (Happy Creek) on John F. Kennedy Space Center were measured using aerial images from 1943, 1951, 1958, 1969, 1979, and 1989. Landcover categories were mapped, digitized, geometrically registered, and overlaid in ARC/INFO. Both study sites have been influenced by various land use histories, including periods of range management, fire suppression, and fire management. Several analyses were performed to help understand the effects of past land management on the amount and spatial distribution of landcover within the study sites. A chi-squared analysis showed a significant difference between the frequency of landcover occurrence and management period. Markov chain models were used to project observed changes over a 100-year period; these showed current management practices being effective at Tel-4 (restoring historic landscape structure) and much less effective at Happy Creek. Documenting impacts of past management regimes on landcover has provided important insight into current landscape composition and will provide the basis for improving land management on Kennedy Space Center and elsewhere. 相似文献
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AIM: Previous studies performed with XBP-01 in vitro indicated that XBP-01 could inhibit vascular smooth muscle cells from being transformed into foam cell and could eliminate the atherosclerotic plaque in C57BL/6J mouse. This experiment is to investigate its mechanism of eliminating plaques in vitro. METHODS: The cultured porcine artery smooth muscle cells incubated with XBP-01 of 0.1 mg/L for 24 h after preincubated with oxidized low density lipoprotein of 15 mg/L for 72 h in vitro. The samples were analyzed by fluorescence microscope, confocal microscope system and flow cytometry. RESULTS: Apoptosis was triggered by being incubated with oxidized low density lipoprotein and this process was accelerated additionally by being incubated with XBP-01. CONCLUSION: XBP-01 can be effective in eliminating atherosclerotic plaque by accelerating the process in which oxidized low density lipoprotein induced smooth muscle cell apoptosis. 相似文献
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Species distribution models (SDMs) often use elevation as a surrogate for temperature or utilise elevation sensitive interpolations
from weather stations. These methods may be unsuitable at the landscape scale, especially where there are sparse weather stations,
dramatic variations in exposure or low elevational ranges. The goal of this study was to determine whether radiation, moisture
or a novel estimate of exposure could improve temperature estimates and SDMs for vegetation on the Illawarra Escarpment, near
Sydney, Australia. Forty temperature sensors were placed on the soil surface of an approximately 12,000 ha study site between
November 2004 and August 2006. Linear regression was used to determine the relationship with environmental factors. Elevation
was correlated more with moderate temperatures (winter maximums, summer minimums, spring and autumn averages) than extreme
temperatures (summer maximums, winter minimums). The correlation (r
2) between temperature and environmental factors was improved by up to 0.38 by incorporating exposure, moisture and radiation
in the regressions. Summer maximums and winter minimums were predominately determined by exposure to the NW and coastal influences
respectively, while exposure to the NE and SW was important during other seasons. These directions correspond with the winds
that are most influential in the study area. The improved temperature estimates were used in Generalised Additive Models for
37 plant species. The deviance explained by most models was increased relative to elevation, especially for moist rainforest
species. It was concluded that improving the accuracy of seasonal temperature estimates could improve our ability to explain
the patchy distribution of many species.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献