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1.
《林业研究》2020,31(4)
Temperate coniferous forest soils are considered important sinks of soil organic carbon(C).Fresh C inputs may,however,affect soil microbial activity,leading to increased organic matter decomposition and carbon dioxide production.Litter consists of labile and recalcitrant fractions which are thought to be utilized by distinct microbial communities and at different rates during the growing season.In this study,we incubated the whole litter(LC+RC),the labile(LC) and the recalcitrant(RC)fractions with the coniferous soil at two temperatures representing spring/autumn(10℃) and summer(20℃)for one month.Soil respiration and microbial community composition were regularly determined using phospholipid fatty acids as biomarkers.The LC fraction greatly increased soil respiration at the beginning of the incubation period but this effect was rather short-term.The effect of the RC fraction persisted longer and,together with the LC+RC fraction,respiration increased during the whole incubation period.Decomposition of the RC fraction was more strongly affected by higher temperatures than decomposition of the more labile fractions(LC and LC+RC).However,when we consider the relative increase in soil respiration compared to the dH_2 O treatment,respiration increased more at a lower temperature,suggesting that available C is more important for microbial metabolism at lower temperatures.Although C was added only once in our study,no changes in microbial community composition were detected,possibly because the microbial community is adapted to relatively low amounts of additional C such as the amounts naturally found in litter.  相似文献   

2.
To investigate the effects of biochar addition(1 or 3%)to the soil of a China fir plantation with or without litter,we conducted a 90-day incubation experiment.We also studied the C and N dynamics and the microbial community structure of the soil.In soil without litter,the application of biochar at a rate of 3%significantly decreased CO2 emissions,while addition of 1%biochar had no effect.Biochar application did not affect the net N mineralization rate but significantly reduced the NH4?concentration after 90 days.In litter-enriched soil,biochar application had no significant effect on total CO2 emissions;however,application of 3%biochar significantly reduced the net N mineralization rate.Biochar application to soil with or without litter immediately reduced the dissolved organic carbon(DOC)concentration independent of the application rate,which was primarily due to sorption of DOC by the biochar.Phospholipid fatty acid analysis demonstrated that both concentrations of added biochar to soil(with or without litter)altered the soil microbial community structure at the end of incubation,although the effect of biochar was not as strong as the effect of time or litter application.The effect of biochar addition alone on microbial community structure was inconsistent over time.Litter added to soil significantly increased fungi and reduced Gram-positive bacteria.In the presence of litter,biochar applied at both 1%and 3%significantly increased(p<0.05)the proportion of actinomycete only at day 90.Our results indicate biochar as a potentially effective measure for C sequestration in the test soil of a China fir plantation,even in the presence of litter.  相似文献   

3.
The effects of soil animals on soil nitrogen (N) mineralization and its availability were studied by investigating soil animal groups and their amounts of macro-faunas sorted by hand, and middle and microfaunas distinguished with Tullgren and Baermann methods under three Pinus sylvestris var. mongolica Litv. plantations in Zhanggutai sandy land, China. In addition, soil N mineralization rate was also measured with PVC closed-top tube in situ incubation method. The soil animals collected during growing season belonged to 13 orders, 5 groups, 4 phyla, whose average density was 86 249.17 individuals-m^-2. There were significant differences in soil animal species, densities, diversities and evenness among three plantations. Permanent grazing resulted in decrease of soil animal species and diversity. The average ammonification, nitrification and mineralization rates were 0.48 g:m^-2·a^-1, 3.68 g·m^-2·a^-1 and 4.16 g·m^-2·a^-1, respectively. The ammonification rate in near-mature forest was higher than that in middle-age forests, while the order of nitrification and net mineralization rates was: middle-age forest without grazing 〈 middle-age forest with grazing 〈 near-mature forest with grazing (P〈0.05). Soil N mineralization rate increased with soil animal amounts, but no significant relationship with diversity. The contribution of soil animals to N mineralization was different for different ecosystems due to influences of complex factors including grazing, soil characteristics, the quality and amount of litter on N mineralization.  相似文献   

4.
A laboratory simulated freeze-thaw was conducted to determine the effects of freeze-thaw on soil nutrient availability in temperate semi-arid regions. Soil samples were collected from sandy soils (0-20 cm) of three typical ecosystems (grassland, Mongolian pine plantation and poplar plantation) in southeastern Keerqin Sandy Lands of China and subjected to freeze-thaw treatment (-12℃ for 10 days, then r 20℃ for 10 days) or incubated at constant temperature (20℃ for 20 days). Concentrations of the soil NO3^--N, NH4^+-N, NaHCO3 extractable inorganic P (LPi) and microbial biomass P (MBP) were determined on three occasions: at the start of the incubation, immediate post-thawing and at the 10th day post-thawing. The results showed that soil net nitrification and N mineralization rates at three sites were negatively affected by freeze-thaw treatment, and decreased by 50%-85% as compared to the control, of which the greatest decline occurred in the soil collected from poplar plantation. In contrast, the concentration of soil NH4^+-N, NaHCO3 extractable inorganic P (LPi) and microbial biomass P were insignificantly influenced by freeze-thaw except that LPi and NH4^+-N showed a slight increase immediate post-thawing. The effects of freeze-thaw on soil N transformation were related to soil biological processes and the relatively constant available P was ascribed to severe soil aridity.  相似文献   

5.
Soil organic carbon(SOC)mineralization is closely related to carbon source or sink of terrestrial ecosystem.Natural stands of Larix olgensis on the Jincang forest farm,Jilin Province were selected to investigate the dynamics of SOC mineralization and its correlations with other soil properties in a young forest and mid-aged forest at soil depths of 0–10,>10–20,>20–40 and>40–60 cm.The results showed that compared with a mid-aged forest,the SOC stock in the young forest was 32%higher.Potentially mineralizable soil carbon(C0)in the young forest was 1.1–2.5 g kg^-1,accounting for 5.5–8.1%of total SOC during the 105 days incubation period and 0.3–1.5 g kg^-1 in the mid-aged forest at different soil depths,occupying 2.8–3.4%of total SOC.There was a significant difference in C0 among the soil depths.The dynamics of the SOC mineralization was a good fit to a three-pool(labile,intermediate and stable)carbon decomposition kinetic model.The SOC decomposition rate for different stand ages and different soil depths reached high levels for the first 15 days.Correlation analysis revealed that the C0 was significantly positively related with SOC content,soil total N(TN)and readily available K(AK)concentration.The labile soil carbon pool was significantly related to SOC and TN concentration,and significantly negatively correlated with soil bulk density.The intermediate carbon pool was positively associated with TN and AK.The stable carbon pool had negative correlations with SOC,TN and AK.  相似文献   

6.
Soil inorganic N is one of the most important soil quality indexes, which may be influenced by land-use change. The historical conversion of land-use from native vegetation to agriculture resulted in sharp declines in soil N dynamics. This study was conducted to determine the soil inorganic N concentrations and net N mineralization rate in four common types of land-uses in the mountain forest area in the north of Iran, namely arable land, pine plantation, ash plantation, and beech stand. The soil samples were taken from top mineral soil layer (5cm) in each site randomly (n=6) during August- September 2010. Beech stand and ash plantation showed significantly higher total nitrogen compared with arable land and pine plantation, while extractable NH 4 + -N concentration was significantly greater in Beech stand compare to arable soils (p<0.05). No significantly difference was found in Net N mineralization, net nitrification and net ammonification rates among different land-uses. Results showed that net N mineralization and ammonification were occurred just in the soil of Ash plantation during the incubation time. Our findings suggested that conversion of Hyrcanian forests areas to pine plantation and agricultural land can disrupt soil natural activities and affect extremely soil quality.  相似文献   

7.
Many studies have estimated approximately ranges of thresholds of low soil temperature in the growth and ecophysiological traits of trees, but difficultly determined the exact values. To resolve the problem, black spruce (Picea mariana) and jack pine (Pinus banksiana) seedlings were exposed to 5, 10, 15, 20, 25, 30 and 35°C soil temperature in greenhouses. After 90 days of the treatment, net photosynthetic rate (A), stomatal conductance (gs), transpiration rate (E), water use efficiency (WUE) and specific l...  相似文献   

8.
The consequence of land-use change from forest to agriculture and other uses has become one of the world’s greatest concerns.The soil,one of the most important components of forests and containing all the required plant nutrients as soluble ions,is highly impacted by these changes.Because vast areas of the Zagros forests in western Iran have changed in use during the last few decades,the present study investigated the effects of landuse changes of forest area to agriculture,orchard,and agroforestry on soil chemical and physical properties.Soil was sampled at four land-use areas:less-disturbed forest areas(control)and agricultural,orchard,and agroforestry areas.Among each of the two forest-use areas(agroforestry and orchard),we selected five trees with similar-sized crowns and sampled under each tree crown at 0-15 and>15-30 cm depths.Five soil samples also were taken in agriculture area at each depth.The findings indicated that during land-use changes,soil sand particles decreased,and clay and silt particles of soil increased,resulting in a fine soil texture.Moreover,the amount of nitrogen(N),phosphorus(P),organic carbon(OC),and electrical conductivity(EC)of soil decreased at both depths due to the decrease in organic matter.Soil pH and magnesium(Mg)level rose during land-use change at both depths except at agricultural sites.Soil potassium(K)content decreased during agricultural use due to the elimination of tree cover.The level of K decreased only at the depth of 0-15 cm because of K dependency on parent materials.Generally,most soil nutrients were affected by plant removal in the conversion.Forest and agricultural soil are distinguishable by their properties,while land-uses such as agroforestry-orchard separated from the others.Soil nutrients were severely affected by the decrease and elimination of tree cover,plowing,and continuous harvesting,resulting in a decline in soil quality and fertility.  相似文献   

9.
The water relation and leaf gas exchange of saxoul (Haloxylon Ammodendron Bge, a C4 shrub) seedlings were studied under water stress in 2001. Saxoul seedlings maintained high transpiration when the soil moisture was above 11%. The seedlings were able to take up water from soil with above 6 % soil water content, which was the threshold level of soil moisture for seedlings. The relationship between transpiration and potential evaporation was linear for well-watered seedlings. The decrease of soil water availability led to different degrees of down-regulation of stomatal conductance, leaf transpiration and net CO2 assimilation rate. The stomata played a relatively small part in determining the net CO2 assimilation rate for the same seedling. The relationship between net CO2 assimilation rate and transpiration was linear diurnally, and reduction scale of leaf transpiration was much bigger than that of net CO2 assimilation rate by waters tress treatments, therefore intrinsic water-use-efficiency increased. High evaporative demand increased the leaf transpiration but inhibited net CO2 assimilation rate.Because of the effect of VPD on transpiration in this region, the transpiration of well-watered and mild water stress seedlings becomes responsive to change in stomatal conductance over a wider range.  相似文献   

10.
Phenolic acids are secondary metabolites of plants that significantly affect nutrient cycling processes.To investigate such effects,the soil available nitrogen(N)content,phenolic acid content,and net N mineralization rate in three successive rotations of Chinese fir plantations in subtropical China were investigated.Net N mineralization and nitrification rates in soils treated with phenolic acids were measured in an ex situ experiment.Compared with first-rotation plantations(FCP),the contents of total soil nitrogen and nitrate in second(SCP)-and third-rotation plantations(TCP)decreased,and that of soil ammonium increased.Soil net N mineralization rates in the second-and third-rotation plantations also increased by 17.8%and 39.9%,respectively.In contrast,soil net nitrification rates decreased by 18.0%and 25.0%,respectively.The concentrations of total phenolic acids in the FCP soils(123.22±6.02 nmol g^-1)were 3.0%and 17.9%higher than in the SCP(119.68±11.69 nmol g^-1)and TCP(104.51±8.57 nmol g^-1,respectively).The total content of phenolic acids was significantly correlated with the rates of net soil N mineralization and net nitrification.The ex situ experiment showed that the net N mineralization rates in soils treated with high(HCPA,0.07 mg N kg^-1 day^-1)and low(LCPA,0.18 mg N kg^-1 day^-1)concentrations of phenolic acids significantly decreased by 78.6%and 42.6%,respectively,comparing with that in control(0.32 mg N kg^-1 day^-1).Soil net nitrification rates under HCPA and LCPA were significantly higher than that of the control.The results suggested that low contents of phenolic acids in soil over successive rotations increased soil net N mineralization rates and decreased net nitrification rates,leading to consequent reductions in the nitrate content and enhancement of the ammonium content,then resulting in enhancing the conservation of soil N of successive rotations in Chinese fir plantation.  相似文献   

11.
杉木纯林、混交林土壤微生物特性和土壤养分的比较研究   总被引:6,自引:0,他引:6  
王清奎  汪思龙 《林业研究》2008,19(2):131-135
本文于2005年5月份,在中国科学院会同森林生态实验站选择了一块15年生的杉木纯林和两块15年生杉阔混交林作为研究对象,调查了林地土壤有机碳、全氮、全磷、硝态氮、有效磷和土壤微生物碳、氮、磷、基础呼吸以及呼吸熵,比较了纯林和混交林土壤微生物特性和土壤养分.结果表明,杉阔混交林的土壤有机碳、全氮、全磷硝态氮和有效磷含量高于杉木纯林;在混交林中,土壤微生物学特性得到改善.在0(10 cm和10(20 cm两层土壤中,杉阔混交林土壤微生物氮含量分别比杉木纯林高69%和61%.在0(10 cm土层,杉阔混交林土壤微生物碳、磷和基础呼吸分别比杉木纯林高11%、14%和4%;在10(20 cm土层,分别高6%、3%和3%.但是,杉阔混交林土壤微生物碳:氮比和呼吸熵较杉木纯林低34%和4%.另外,土壤微生物与土壤养分的相关性高于土壤呼吸、微生物碳:氮比和呼吸熵与土壤养分的相关性.由此可知,在针叶纯林中引入阔叶树后,土壤肥力得以改善,并有利于退化森林土壤的恢复.  相似文献   

12.
Sugi heartwood was acetylated with acetic anhydride in supercritical carbon dioxide (CO2) (120°C or 130°C, 10–12 MPa). As a result, the weight percent gain increased with increasing acetylation time up to 16%–20% at 1 h and 24%–28% at 24 h. The antiswelling efficiency of the acetylated specimens reached 75%–80% at 3–4 h of acetylation. It is supposed that the acetylation in supercritical CO2 has a high bulking effect compared with liquid-phase and vapor-phase acetylation with uncatalyzed acetic anhydride. The results showed that the acetylation progressed rapidly because supercritical CO2 and acetic anhydride formed a single phase at more than 90°C, and the acetic anhydride reached the reaction sites in the wood quickly.  相似文献   

13.
A study was conducted to investigate the effects of high temperature on variations of alkaloid metabolism in C. roseus seedlings in Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Heilongiang, China. 60-day-old C. roseus seedlings with 3-4 pairs of leaves were incubated in chambers with temperature of 30℃ and 40℃for short-term heat shock experiment and 20℃, 25℃ and 35℃ for long-term experiment. The contents of vindoline, catharanthine, vinblastine and vincristine in C. roseus leaves and root were checked at different temperatures in short term (1-6 h) and long term (1-16 d). Results showed that under short-term heat shock, the contents of vindoline, catharanthine and vinblastine in leaves of the seedlings were higher at 40℃ than at 30℃, but after 6 h treatment, the contents of vindoline and catharanthine under the two temperatures came to the same level. Catharanthine was exclusively distributed in C. roseus roots and its content was increased by 40% after two hours incubation at 40℃, while increased slowly at 30℃ incubation and reached the highest value at 6 h. In the Long-term experiment, concentrations of monomeric alkaloids catharanthine and vindoline were higher at 20℃ than at 25℃ and had a sharp increase under the condition of 35℃. While for dimeric alkaloid, it showed that the higher the temperature, the earlier the peak value of vinblastine content appears. Vincristine had a continuous enhancement and attained 0.027 mg.g^-1 at 16th day under 35℃ condition which was higher than those in the other conditions. It was concluded that high temperature could promote the accumulation of different alkaloids in C. roseus and the accumulation characteristic is highly related to treatment time.  相似文献   

14.
随着大气CO2浓度的升高,主要由其引起的温室效应与对生物新陈代谢的影响变得越来越显著。森林生态系统在全球碳循环中扮演着重要的角色。为了评估和理解森林土壤CO2通量及其随空气和土壤温度的季节和昼夜变化规律,我们在长白山北坡典型阔叶红松林内利用静态箱技术进行了原位观测。实验在整个生长季(6月初至9月末)昼夜进行,利用气相色谱进行气体分析。结果表明: 长白山阔叶红松林土壤是大气二氧化碳源,其CO2通量具有明显的季节和昼夜变化规律。通量的变化范围是(0.30-2.42)μmol穖-2穝-1,平均值为0.98μmol穖-2穝-1。土壤CO2排放的季节规律表明,土壤CO2通量的变化与气温和土壤温度的变化有关。CO2平均通量的最大值出现在7月((1.27±23%)μmol穖-2穝-1),最小值出现在9月((0.5±28%)μmol穖-2穝-1)。土壤CO2的昼夜波动与土壤温度变化有关,而在时间上滞后于温度的变化。森林下垫面土壤CO2通量与土壤温度显著相关,与6cm深度土层温度相关系数最大。基于气温和土壤温度计算的Q10值范围为2.09-3.40。图2表3参37。  相似文献   

15.
Understanding the germination traits of plants is important not only for understanding natural regeneration processes but also for developing seedling production techniques for planting. Sabina vulgaris Ant. is a common species used for reforestation in semi-arid areas of the Mu-Us Desert, in Inner Mongolia, China, but its extremely low germination rate, both in situ and in vivo, is a bottleneck for seedling production. Sulfuric acid pretreatment was applied to improve germination, and the germination rate was compared for different soaking time (10, 30, 60, 90, and 120 min), different temperatures (10, 15, 20, 30, and 35°C) and under different lighting conditions (dark and light). Sulfuric acid treatment gave a high germination rate, reaching 60% at 30 days after sowing. However, the non-treated seeds produced no germination. The optimal treatment time in sulfuric acid was 120 min. Germination after sulfuric acid treatment increased at incubation temperatures from 10 to 30°C, but decreased at 35°C. Incubation at 25–30°C gave maximum germination of more than 50%. Light treatment had little effect on germination. Pretreatment with sulfuric acid improved water absorption by the embryo by creating cracks and cavities in the seed coat tissue. These results indicated that S. vulgaris seeds have physical dormancy caused by their hard seed coats, which prevents absorption of water into the embryo. A combination of pretreatment with sulfuric acid and incubation at 25–30°C was most effective in improving the germination of S. vulgaris seeds.  相似文献   

16.
土壤温度和水分对长白山3种温带森林土壤呼吸的影响   总被引:7,自引:0,他引:7  
为了研究土壤温度和土壤含水量对阔叶红松林(山地暗棕壤)、云冷杉暗针叶林(山地棕针叶林土壤)和岳桦林(生草森林土)的土壤呼吸的影响,于2001年9月在长白山进行了土壤实验。利用增加土壤样柱的含水量,将土壤含水量分为9%,、21%、30%、37%和43%5个等级,土壤样品分别在0、5、15、25和35的温度下保持24小时。阔叶红松林土壤在0~35范围内,土壤呼吸速率与温度呈正相关。在一定的含水量范围内(21%~37%),土壤呼吸随含水量的增加而升高,当含水量超出该范围,土壤呼吸速率则随含水量的变化而降低。土壤温度和水分对土壤呼吸作用存在明显的交互作用。不同森林类型土壤呼吸作用强弱存在显著差异,大小顺序为阔叶红松林>岳桦林>云冷杉暗针叶林.红松阔叶林土壤呼吸作用的最佳条件是土壤温度35,含水量37%;云冷杉暗针叶林下的山地棕色针叶土壤呼吸作用的最佳条件是25,21%;岳桦林土壤呼吸作用的最佳条件是35,含水量37%。但是,由于长白山阔叶红松林,云冷杉林和岳桦林处在不同的海拔带上,同期不同森林类型土壤温度各不相同,相差4~5,所以野外所测的同期的山地棕色针叶林土呼吸速率应低于暗棕色森林土呼吸速率,山地生草森林土呼吸速率应高于山地棕色针叶林土的呼吸速率。图2表1参25。  相似文献   

17.
A study was conducted to determine the effects of elevated CO2 on soil N process at Changbai Mountain in Jilin Province, northeastern China (42°24′N, 128°06′E, and 738 m elevation). A randomized complete block design of ambient and elevated CO2 was established in an open-top chamber facility in the spring of 1999. Changpai Scotch pine (Pinus sylvestris var. sylvestriformis seeds were sowed in May, 1999 and CO2 fumigation treatments began after seeds germination. In each year, the exposure started at the end of April and stopped at the end of October. Soil samples were collected in June and August 2006 and in June 2007, and soil nitrifying, denitrifying and N2-fixing enzyme activities were measured. Results show that soil nitrifying enzyme activities (NEA) in the 5–10 cm soil layer were significantly increased at elevated CO2 by 30.3% in June 2006, by 30.9% in August 2006 and by 11.3% in June 2007. Soil denitrifying enzyme activities (DEA) were significantly decreased by elevated CO2 treatment in June 2006 (P < 0.012) and August 2006 (P < 0.005) samplings in our study; no significant difference was detected in June 2007, and no significant changes in N2-fixing enzyme activity were found. This study suggests that elevated CO2 can alter soil nitrifying enzyme and denitrifying enzyme activities. Foundation project: This research was supported by the National Natural Science Foundation of China (No. 90411020) and Major State Basic Research Development Program of China (973 Program) (2002CB412502).  相似文献   

18.
本文研究了大叶桃花心木(Swietenia macrophylla King)一年生幼苗在经过夜温处理后的光响应曲线和在饱和光强下的CO2反应曲线.结果表明:在大气CO2浓度下,叶片的最佳光合作用温度在25-31℃之间,而在饱和CO2浓度下为31-35℃.在25℃以下光合速率开始降低,主要是由于羧化效率的降低,而当温度超过31℃时,光合速率下降,是因为羧化效率的降低和呼吸速率的增加.CO2浓度对光合的促进作用在低温下受到抑制,这意味着未来在CO2浓度增高的情况下,高浓度的CO2对热带常绿植物光合的促进在冬天低温情况下表现不十分明显.图4参23.  相似文献   

19.
Branch CO2 efflux of Norway spruce tree [Picea abies (L.) Karst.] was measured in ten branches at five different whorls during the growing season 2004 (from June till October) in campaigns of 3–4 times per month at the Beskydy Mts., the Czech Republic. Branch CO2 efflux was measured using a portable infrared gas analyzer (LI-6250, LI-COR, Inc., USA), operating as a closed system. Branch woody-tissue temperature was measured continuously in 10-min intervals for each sample position during the whole experiment period. On the basis of relation between CO2 efflux rate and woody-tissue temperature, a value of Q10 and of normalized CO2 efflux rate (E10–CO2 efflux rate at 10°C) was calculated for each sampled position. Estimated Q10 values ranged from 2.12 to 2.89, and E10 ranged from 0.41 to 1.19 μmolCO2m−2s−1. Differences in branch CO2 efflux were found between orientations, east-side branches presented higher efflux rate than west-side branches. The highest branch CO2 efflux rate values were measured in August and the lowest in October, which corresponds with woody-tissue temperature and growth processes during these periods. Branch CO2 efflux was significantly and positively correlated with branch position within canopy and woody-tissue temperature. Branches from the upper whorls showed higher CO2 efflux activity and seasonal dynamics than branches from the lower whorls.  相似文献   

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