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1.
为探寻可能引发鸡支气管阻塞的病毒类病原,试验采用RT-PCR方法检测了自河北及周边地区收集的43份有支气管阻塞病料样品中H5亚型禽流感病毒(AIV)、H7亚型AIV、H9亚型AIV、传染性支气管炎病毒(IBV)和新城疫病毒(NDV)等病毒类病原感染情况。结果显示,阳性样品共24份,总阳性率为55.8%(24/43),其中H9亚型AIV、IBV、H5亚型AIV、H7亚型AIV、NDV的阳性率分别为34.9%(15/43)、20.9%(9/43)、0(0/43)、0(0/43)、0(0/43),不存在H9亚型AIV和IBV的混合感染;自检测阳性样品中分离、鉴定了5株H9N2 AIV,HA蛋白裂解位点模式均为PSRSSR↓GLF,属于低致病性毒株,位于h9.4.2.5分支,3株分离毒株HA蛋白关键氨基酸位点模式与疫苗毒株WJ57完全一致,但5株分离毒株NA蛋白关键氨基酸位点模式与疫苗毒株WJ57不完全一致;自检测阳性样品分离、鉴定了4株IBV,S1蛋白裂解位点模式包括HRHRR和HRRRR,属于LX4型,均与疫苗毒株YX10 D90亲缘关系较近,与疫苗毒株Connecticut和H120亲缘... 相似文献
2.
胶东地区规模鸡场3种主要病毒感染情况的调查 总被引：3，自引：1，他引：2
应用RT-PCR对山东青岛、潍坊和烟台3个地区2008年1月至2009年6月送检的1837份具有呼吸道症状鸡的病料进行新城疫病毒(NDV)、传染性支气管炎病毒(IBV)和H9亚型禽流感病毒(AIV)3种主要病毒感染情况的调查。调查显示,3个地区3种病毒的感染率可达65.76%,其中NDV、IBV和AIV(H9)的平均阳性率分别为12.36%、12.30%和41.10%。青岛地区NDV、AIV(H9)和IBV的阳性率最高,分别为13.45%、17.37%和44.08%。2008年下半年较2008年上半年3种病毒的检出率均有所升高,以AIV(H9)升高最为显著;2009年上半年较2008年上半年AIV(H9)阳性率上升最为明显。3种病毒混合感染的阳性率可达11.00%,以IBV+AIV(H9)和NDV+AIV(H9)二重混合感染临床最为常见。表明山东省胶东地区NDV、IBV和AIV(H9)的感染情况比较普遍,且存在一定程度的混合感染。本调查结果为山东省制定规模鸡场3种疫病的防控措施提供了科学资料。 相似文献
3.
为了解2009下半年山东胶东地区规模化鸡场主要疫病的感染情况,应用RT-PCR技术对山东青岛、潍坊和烟台三地区送检的774份具有呼吸道症状的病鸡病料进行了新城疫病毒(NDV)、传染性支气管炎病毒(IBV)和H9亚型禽流感病毒(AIV)3种主要病毒的感染情况调查。调查结果显示,三个地区3种病毒的感染率为25.58%,NDV、IBV和AIV(H9)的阳性率分别为8.14%、11.37%和6.07%。青岛地区NDV、IBV的阳性率均最高,分别为12.47%、14.55%。2种病毒混合感染的阳性率达2.84%,以IBV+NDV二重混合感染临床最为常见。调查结果表明,山东省胶东地区NDV、IBV和AIV(H9)的感染情况比较普遍,且存在一定程度的混合感染,为有效预防和控制山东省规模鸡场该3种主要疫病提供了科学依据。 相似文献
4.
H9N2亚型禽流感病毒（Avian influenza virus,AIV）属于低致病性AIV,但因其分布广泛、传播迅速,可引起感染家禽生产性能下降,给家禽业带来了极大的经济损失。H9N2亚型AIV在感染家禽过程中可引起严重的免疫抑制,使家禽极易继发上呼吸道细菌、消化道细菌等感染,从而导致H9N2亚型AIV致病力增强,细菌黏附定植能力增强,家禽死亡率显著升高。另外,H9N2亚型AIV还能与禽传染性支气管炎病毒、禽传染性法氏囊病病毒、新城疫病毒等发生混合感染,病毒入侵时有可能出现协同作用或颉颃作用,从而相互促进或抑制病毒的复制和排毒;H9N2亚型AIV还极易发生突变或与其他亚型流感病毒在混合感染时发生基因重组产生感染人的新亚型毒株,给人类健康和公共卫生安全带来重大威胁。作者综述了H9N2亚型AIV与其他病原混合感染的研究进展,通过阐述H9N2亚型AIV与细菌或病毒混合感染的协同或颉颃作用,以期为临床上H9N2亚型AIV混合感染的防治提供参考。 相似文献
5.
为了解2008年1月～2012年6月山东胶东青岛、潍坊和烟台三地区新城疫病毒(NDV)、传染性支气管炎病毒(IBV)和H9亚型禽流感病毒(AIV(H9))的感染情况,采用RT-PCR对送检的5 001份患有呼吸道症状的病料进行了检测.结果显示,3地区3种病毒的总阳性率为48.49％,其中AIV(H9)感染率最高,为26.15％.青岛地区NDV和IBV的阳性率均最高,分别为15.94％和11.63％,潍坊地区AIV(H9)阳性率最高(29.23％).4年半间AIV(H9)感染下降明显,但NDV近3年感染明显抬头.寒冷月份3种病毒感染较高,3月份NDV和AIV(H9)阳性率均最高,分别为21.07％和38.17％;10月份IBV阳性率最高为15.40％.3种病毒混合感染阳性率为6.66％,以NDV+ AIV(H9)二重感染最常见(2.98％),NDV+IBV+AIV(H9)三重感染为0.40％.结果表明,山东胶东地区NDV、IBV和AIV(H9)的感染情况较普遍,存在一定程度的混合感染,为有效防控山东省规模鸡场该3种主要疾病提供了依据. 相似文献
6.
为了解2009下半年山东胶东地区规模化鸡场主要疫病的感染情况,应用RT.PCR技术对山东青岛、潍坊和烟台三地区送检的774份具有呼吸道症状的病料进行了新城疫病毒(NDV)、传染性支气管炎病毒(IBV)和H9亚型禽流感病毒(AIV)三种主要病毒的感染情况调查.调查结果显示三个地区三种病毒的感染率为25.58%,NDV、IBV和AIV(H9)的阳性率分别为8.14%、11.37%和6.07%.青岛地区NDV、IBV的阳性率均最高,分别为12.47%、14.55%.二种病毒混合感染的阳性率可达2.84%,以IBV+NDV二重混合感染临床最为常见.调查结果表明山东省胶东地区NDV、IBV和AIV(H9)的感染情况比较普遍,且存在一定程度的混合感染,这为有效预防和控制山东省规模鸡场该三种主要疾病提供了科学依据. 相似文献
7.
8.
为了解2009下半年山东胶东地区规模化鸡场主要疫病的感染情况,应用RT-PCR技术对山东青岛、潍坊和烟台三地区送检的774份具有呼吸道症状的病料进行了新城疫病毒(NDV)、传染性支气管炎病毒(IBV)和H9亚型禽流感病毒(AIV)三种主要病毒的感染情况调查。调查结果显示三个地区三种病毒的感染率为25.58%,NDV、IBV和AIV(H9)的阳性率分别为8.14%、11.37%和6.07%。青岛地区NDV、IBV的阳性率均最高,分别为12.47%、14.55%。二种病毒混合感染的阳性率可达2.84%,以IBV+NDV二重混合感染临床最为常见。调查结果表明山东省胶东地区NDV、IBV和AIV(H9)的感染情况比较普遍,且存在一定程度的混合感染,这为有效预防和控制山东省规模鸡场该三种主要疾病提供了科学依据。 相似文献
9.
《中国动物检疫》2010,(9)
为了解2009下半年山东胶东地区规模化鸡场主要疫病的感染情况,应用RT-PCR技术对山东青岛、潍坊和烟台三地区送检的774份具有呼吸道症状的病料进行了新城疫病毒(NDV)、传染性支气管炎病毒(IBV)和H9亚型禽流感病毒(AIV)三种主要病毒的感染情况调查。调查结果显示三个地区三种病毒的感染率为25.58%,NDV、IBV和AIV(H9)的阳性率分别为8.14%、11.37%和6.07%。青岛地区NDV、IBV的阳性率均最高,分别为12.47%、14.55%。二种病毒混合感染的阳性率可达2.84%,以IBV+NDV二重混合感染临床最为常见。调查结果表明山东省胶东地区NDV、IBV和AIV(H9)的感染情况比较普遍,且存在一定程度的混合感染,这为有效预防和控制山东省规模鸡场该三种主要疾病提供了科学依据。 相似文献
10.
《黑龙江畜牧兽医》2017,(20)
根椐Gen Bank中已发表的白色念珠菌(CA)、传染性支气管炎病毒(IBV)、新城疫病毒(NDV)基因序列设计并合成三种病原的特异性检测引物,通过三重PCR反应条件的优化,建立了检测三种病原的PCR检测方法。该方法对同一样品中的CA、IBV和NDV核酸模板可同时扩增出231 bp、417 bp和747 bp的特异性片段,检测光滑念珠菌(CG)、克柔念珠菌(CK)、热带念珠菌(CT)、H9亚型禽流感病毒(AIV H9)、鸡传染性法氏囊病毒(IBDV)、鸡传染性喉气管炎病毒(ILTV)、减蛋综合症病毒(EDSV)等7种禽病病原均为阴性,该方法对CA DNA、IBV c DNA、NDV c DNA的检测最低限度分别为25.0,33.5,36.5 pg,临床样品检测结果表明三重PCR检测方法可以用于这三种病原单独和混合感染的检测和鉴别诊断。说明所建立的三重PCR检测方法具有很好的特异性、敏感性、重复性和临床适用性,为三种病原单独或混合感染的检测和鉴别诊断提供了一种操作简单、检测快速的分子生物学诊断方法。 相似文献
11.
生长阻滞和DNA损伤诱生蛋白45β（growth arrest and DNA damage 45β，GADD45β）参与多种细胞信号通路，在病毒感染过程中发挥重要作用，但在禽白血病病毒（avian leukosis virus，ALV）感染中研究较少。本研究中，用J亚群禽白血病病毒（ALV-J）感染DF-1细胞，通过荧光定量PCR和Western blot检测GADD45β表达水平。此外，在过表达GADD45β和干扰GADD45β的情况下，通过Western blot、间接免疫荧光试验和ELISA检测GADD45β对ALV-J复制的影响。结果表明，ALV-J感染DF-1细胞能显著上调GADD45β表达水平（P<0.05）。过表达GADD45β后，ALV-J的蛋白表达水平显著降低（P<0.05），荧光信号强度也明显低于对照组。然而，干扰GADD45β后，ALV-J的复制水平显著上调（P<0.05），说明GADD45β可以抑制ALV-J病毒复制。本研究首次发现GADD45β具有抑制ALV-J病毒复制的功能，为抗ALV-J的研究提供了新的思路和理论基础。 相似文献
12.
Development and characterization of monoclonal antibodies to subgroup J avian leukosis virus. 总被引：6，自引：0，他引：6
In an attempt to develop a specific diagnostic test for avian leukosis virus (ALV) subgroup J (ALV-J) strain Hc1, four monoclonal antibodies (MAbs), JE9, G2, 145, and J47, were generated that are specific for ALV-J envelope glycoprotein, gp85. Polymerase chain reaction (PCR) was used to amplify genomic pro-viral DNA of Avian Disease and Oncology Laboratory (ADOL)-Hc1 and ADOL-4817 envelope genes. Both open reading frames encoding glycoproteins gp85 and gp37 were cloned into baculoviruses. Abundant expression of gp85 and gp37 was detected in the recombinant viruses with specific antibody to Hc1 strain of the ALV-J. The expressed proteins were used for immunization of mice to produce hybridoma cell lines secreting MAbs specific to ALV-J envelope protein. A panel of MAbs was generated by fusing NS1 myeloma cells and spleen cells from mice immunized with the recombinant baculoviruses. With the use of an immunofluorescence assay, three MAbs (JE9, G2, 145) reacted with ALV-J but not with subgroups A, B, C, D, or E of ALV. MAb J47 reacted with all exogenous subgroups of ALV including A, B, C, D, and J but not with endogenous subgroup E viruses. Western blot analysis was performed with all four MAbs against recombinant baculovirus and Hc1-infected chicken embryo fibroblast (CEF) lysates. A major band with a molecular weight about 90 kD corresponding to the size of ALV-J envelope was consistently obtained. With these MAbs, we detected the Hc1 antigen in CEFs infected with several ALV-J viruses isolated in the United States and also in tissue sections from chickens infected with Hc1 strain of ALV-J. These MAbs will be useful reagents for the diagnosis of ALV-J infection because they recognize a common antigenic epitope in six isolates tested thus far. 相似文献
13.
研究禽网状内皮组织增生症病毒(REV)、禽J亚群白血病病毒(ALV-J)感染及混合感染引起血清指标动态变化与鸡群体重抑制的关系.3组1日龄哈伯德父母代肉种鸡,分别接种REV、LV-J和REV与ALV-J.定期称量体重,采外周静脉血,通过生化自动分析仪检测鸡血清中总蛋白(TP)、高密度脂蛋白胆固醇(HDL-c)、低密度脂蛋白胆固醇(LDL-c)、甘油三酯(TG)、胆固醇(CHO)的动态变化.结果表明,前7周,血清学指标TP、TG、LDL-c浓度变化与生长抑制的变化呈现相关性,随体重的增加,TP、TG、LDL-c浓度有上升的趋势,HDL-c、CHO浓度变化与体重的变化无相关性.因此,检测7周内的血清TP、TG、LDL-c浓度的动态变化与鸡群体重抑制的关系可作为这两种肿瘤性疾病早期诊断的依据. 相似文献
14.
Thu WL Wang CH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(3):325-328
Subgroup J avian leucosis virus (ALV-J) causes great economic losses in the poultry industry. One in 3 grandparent farms was closed due to ALV-J infection in 1998 in Taiwan. The remaining 2 farms were forced to import breeding chicks from different breeding companies afterwards. We report on the ALV-J infection status among these breeders, their progeny and Taiwan native chickens during 2000-2002. The weekly mortality for the male line among the infected breeders was higher than that for the female line. Sixty-three percent (5/8) of the broiler flocks were infected with ALV-J. The surface (SU) portion of the env gene from the ALV-J field isolates was cloned and sequenced. The phylogenetic results show that all of the isolates fell into 2 clusters. Unexpectedly, the isolates from the same breeds fell into different clusters, with a cluster including isolates from different breeding companies. ALV-Js from native chickens crossbred with imported chickens were placed into the same clusters as those from the imported breeds. The high similarities observed in different ALV-J isolates suggest that different ALV-Js were mixed in the pedigree generations in different breeding lines. 相似文献
15.
采用SP法检测商品蛋鸡感染J亚群禽白血病病毒对产蛋性能的影响 总被引：6，自引：0，他引：6
本研究属世界上首次报道J亚群禽白血病病毒感染对蛋鸡产蛋性能的影响。尸体剖检观察到产蛋鸡的生殖系统发育不良，表现为卵巢、输卵管幼稚型，输卵管粗细不均。组织病理学观察，发现卵巢组织中有大量的胞浆内充满球形嗜酸性颗粒的骨髓瘤细胞。用特异性抗J亚群禽白血病病毒(ALV—J)囊膜糖蛋白gp85的单克隆抗体检测不产蛋鸡的卵巢、输卵管等待检的组织切片，均检出病毒阳性抗原。结果表明ALV—J的感染造成产蛋鸡卵巢、输卵管发育不良，是蛋鸡不产蛋的直接原因。 相似文献
16.
应用鸡包涵体肝炎病毒 FAV- Hb株试验感染 SPF鸡胚 ,通过透射电镜对感染鸡胚肝脏的观察 ,表明 FAV- Hb株可引起鸡胚肝细胞内形成三种类型的包涵体 ,即非病毒性中等电子密度包涵体 ,病毒性包涵体和非病毒性高电子密度包涵体 ,各型包涵体均可见于胞核或胞浆内 ,但胞核是包涵体首先形成的部位 ,核内包涵体通过核膜进入胞浆。各型包涵体在其形成过程中有较密切的关系。 相似文献
17.
Feather pulp from experimentally infected chickens was used as a source of DNA for polymerase chain reaction (PCR) amplification of avian leukosis virus subgroup J (ALV-J) proviral DNA. A primer set that produces a large amplicon (approximately 2,125) was used to detect ALV-J proviral DNA. This primer set was used in lieu of previously published primers because it allows for sequencing of the entire envelope gene and because it was able to detect diagnostically a number of North American ALV-J isolates that could not be detected with previously published primers and PCR conditions. ALV-J proviral DNA was detected in feather pulp at 7 days of age in more than 90% of birds infected as embryos and 7 days postinoculation in over 50% of chickens infected at 3 days of age. The results obtained with PCR on feather pulp were compared with those of virus isolation. In the embryo-inoculated birds, the percentages of agreement between PCR and virus isolation were 92.5% at 7 days of age and 100% at 28, 42, 49, and 56 days of age. However, the overall sensitivity of virus isolation in embryo-infected birds was higher, particularly at 7 and 56 days of age. In chickens inoculated at 3 days of age, the percentages of agreement of detection between PCR and virus isolation ranged from 75% at 10 days of age to 100% at 42 days of age. Agreement of negative results of ALV-J detection by PCR and virus isolation in chickens infected posthatch ranged between 66.6% and 100% between the ages of 10 and 42 days. Virus isolation requires chicken embryo fibroblasts of specific genetic lines, and the process takes onaverage 7-9 days. Aseptic collection of blood and tissues for virus isolation and molecular detection of ALV-J requires sterile necropsy instruments as well as syringes and needles for each individual chicken, whereas sterile microcentrifuge tubes and gloves are the only equipment necessary for aseptic feather pulp collection for ALV-J detection by PCR. PCR-based detection of ALV-J in feather pulp is especially suitable when ALV-J infection must be diagnosed rapidly and unequivocally without killing the chicken(s) and in situations where crucial reagents or suitable virus propagation substrates are not readily available for isolation and propagation of ALV-J in cell culture. 相似文献
18.
19.
Isolation and characterization of emerging subgroup J avian leukosis virus associated with hemangioma in egg-type chickens 总被引：1，自引：0，他引：1
Lai H Zhang H Ning Z Chen R Zhang W Qing A Xin C Yu K Cao W Liao M 《Veterinary microbiology》2011,151(3-4):275-283
Subgroup J avian leukosis virus (ALV-J), first isolated in 1989, predominantly causes myeloid leukosis (ML) in meat-type or egg-type chicken. Since 2006, the clinical cases of hemangioma rather than ML in commercial layer flocks associated with ALV-J have been reported, but it was still not clear whether the novel oncogenic ALV-J had emerged. We characterized SCAU-HN06 isolate of ALV-J from hemangioma in commercial Roman layers through animal experiment and full-length proviral genome sequence analysis. The SPF white leghorn egg-type chickens infected with SCAU-HN06 in ovo at day 11 of incubation showed an overall incidence of 56% hemangioma and 8% renal tumor throughout the 22-week trial, the mortality rate was 16%. Most genes of SCAU-HN06 isolate showed high nucleotide sequence identity to JS09GY6 which was isolated from Hy-Line Variety Brown layers suffering hemangioma. The 19-bp insertion in leader sequence and one key deletion in E element were the common features of SCAU-HN06 and JS09GY6. SCAU-HN06 and those ALV-Js associated with hemangioma, possibly recombinants of ALV-J and other avian retrovirus, may share the same ancestor. 相似文献
20.
两个规模化商品蛋鸡场发病鸡群临床解剖显示：脚部和翅膀有血管瘤、肌肉组织内出现纤维肉瘤,肿大的肝、脾出现髓细胞瘤;经组织病理学观察符合J亚群禽白血病（ALV-J）的病理学特征。针对ALV—J保守序列P27基因设计特异引物,建立PCR方法,从出现病理改变的各组织脏器、各多发性肿瘤中以及血液中均检测到ALV-J,证实该病为以髓细胞瘤,血管瘤和纤维肉瘤为主的多发性肿瘤传染病。对扩增产物序列进行测定后,与原型株序列进行比较,核苷酸同源性为98．27％,从分子水平上证明为J亚群禽白血病,用PCR检测进一步验证J亚群禽白血病毒的感染机制和引起鸡群的病理改变。 相似文献