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1.
A 5-month-old mixed-breed filly presented with diarrhea due to Salmonella typhimurium infection and subsequently developed pneumonia in addition to ischemic necrosis of distal limbs. Pulmonary lesions were characterized by numerous discrete, disseminated pyogranulomas with intralesional fungal hyphae. The morphologic characteristics of fungal hyphae were consistent with Aspergillus spp., and large numbers of A fumigatus were isolated from lung tissue via fungal culture. Lesions in all 4 limbs were similar in distribution, duration, and severity and were characterized by coagulation necrosis accompanied by occasional thrombi in small vessels without evidence of thrombosis of larger limb arteries. Thus, limb lesions are consistent with symmetrical peripheral gangrene, a potential complication of sepsis. 相似文献
2.
从细胞角度揭示苜蓿假盘菌与苜蓿叶片亲和性互作机制。以两者亲和性互作系统为研究材料,利用透射电子显微镜技术研究了苜蓿假盘菌与苜蓿叶片亲和性互作的超微结构特征。结果表明,病菌侵入寄主组织后,菌丝直接穿透寄主细胞壁进入寄主细胞形成胞内菌丝,以胞内生长并向相邻细胞扩展。病菌菌丝穿透寄主细胞壁时,菌丝中的液泡给予了较大的压力帮助其穿透。进入寄主细胞内的病菌菌丝,被内陷的寄主原生质膜包裹,菌丝与质膜始终是隔离的,寄主原生质膜和细胞壁之间沉积电子致密度深的物质。病菌菌丝不断地在寄主原生质膜区域扩展,伴随菌丝体在寄主细胞内的不断扩大,周围的原生质膜也相应扩大其面积,但始终将寄主原生质与菌丝体隔开,而脱离质膜的菌丝形成菌丝鞘包裹。随侵染程度的增加,未被穿透的寄主原生质膜区域逐步被降解。病菌侵染叶绿体等细胞器时,首先是菌丝鞘与叶绿体等细胞器膜相连,然后降解其基粒片层结构,被降解的细胞器组织沿菌丝和胞壁周围沉积。侵染后期,菌丝胞内和胞外扩展,但处在细胞降解物中的菌丝显示较厚的细胞壁,寄主细胞内充满了大量的黑色物质和结晶状的颗粒物。 相似文献
3.
Cerebral phaeohyphomycosis in a dog 总被引：2，自引：0，他引：2
G Migaki H W Casey W B Bayles 《Journal of the American Veterinary Medical Association》1987,191(8):997-998
Cerebral phaeohyphomycosis was diagnosed in a 9-year-old spayed dog that had a series of epileptic convulsions a day before death. About 6 weeks before her death, she had been treated for severe demodectic mange. During this period, persistent leukopenia, lymphocytopenia, and thrombocytopenia were found by blood analyses. At necropsy, multiple large pyogranulomatous lesions were found in the cerebrum and meninges. Dematiaceous fungi with brown, branching, septate hyphae and budding yeasts were found within tissue cells and in the necrotic areas. 相似文献
4.
Silva SM Castro RS Costa FA Vasconcelos AC Batista MC Riet-Correa F Carvalho EM 《Veterinary pathology》2007,44(3):314-319
Conidiobolomycosis is reported in the state of Piauí, in the semiarid region of northeastern Brazil. Affected sheep had depression, weight loss, serous or mucohemorrhagic nasal discharge, and cranium-facial asymmetry from exophthalmos of 1 eye, generally with increased volume of the eyeball, keratitis, and corneal ulceration. At necropsy of 60 sheep, friable masses were observed in the posterior region of the nasal cavity, often destroying the ethmoturbinate bones. Frequently, the lesions invaded the nasal sinuses, cribiform plate, orbit, and brain. The masses were irregular, granular with moist surfaces, and soft and friable with white, yellow, or tan coloration. Dissemination of the lesion to lungs was observed in 27 sheep, to the brain in 26, to lymph nodes in 3, to the kidney in 2, and to the gallbladder and heart in 1. The microscopic examination showed granulomatous inflammation composed of central necrosis surrounded by lymphocytes, epithelioid and giant cells, and fibrous tissue. In all lesions, negatively stained structures representing hyphae were surrounded by Splendore-Hoeppli material. Coagulative necrosis, thrombosis, and vasculitis were also observed. Grocott methenamine silver stain showed 8-30-microm-thick hyphae, rarely septate or ramified, irregular in shape, and with black contoured wall, sometimes with bulbous dilatation in the extremities. On electron microscopy, the hyphae had a thick double wall surrounded by cellular remnants and an inflammatory exudate. Conidiobolus coronatus was isolated from the lesions of 6 sheep. Conidiobolomycosis is an important disease of sheep in the state of Piauí, and other regions of northeastern Brazil. 相似文献
5.
A case of subcutaneous phycomycosis of a horse from which Basidiobolus haptosporus was isolated is described. Although some clinical signs were similar to the disease caused by Hyphomyces destruens , the gross and microscopic pathology were different in several respects. Main characteristics of the histopathology were the narrow eosinophilic sleeve around wide, frequently septate hyphae scattered throughout the affected tissue and found commonly at the advancing border of the lesion. A retrospective study of 63 cases of phycomycosis diagnosed at this laboratory since 1970 indicated a further 11 cases with similar histological features. The significance of this finding for the epidemiology and pathogenesis of equine dermal granulomas is discussed. 相似文献
6.
Fumiko SUZUTA Kumiko KIMURA Ryo URAKAWA Yukio KUSUDA Shogo TANAKA Yasuko HANAFUSA Makoto HARITANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):1029-1031
This report presents a new case of mucormycosis encountered in penguin characterized by morphological variation of hyphae and presence of sporangia with numerous sporangiospores. A 4.5-year-old Magellanic penguin (Spheniscus magellanicus) died after exhibiting anorexia, poor nutritional condition and dyspnea. Multiple nodular lesions were observed in the thoracic and abdominal regions. Histopathologically, hyphae of various sizes were seen in the lungs, air sac and nodular lesions. Myriad sporangiospores and several sporangia were observed in/around the bronchi or parabronchi. The very narrow and short hyphae in the nodules were not consistent with the characteristics of Mucorales. However, for most hyphae, including those in the nodules, sporangiospores and sporangia, immunohistochemistry revealed Mucorales-positive reactions. In addition, these fungi were identified as Rhizomucor pusillus by gene
analysis. 相似文献
7.
An immunohistochemical study of 25 lesions from 7 dogs with disseminated aspergillosis (Aspergillus terreus) is presented. All had multiple fungal granulomas in many viscera, with centres of necrotic tissue and hyphal elements surrounded by a mixed infiltrate of predominantly mononuclear cells. Within these lesions, hyphae coated with immunoglobulin (IgG, IgM, IgA) and complement (C3, C4) were identified, together with peri-lesional mononuclear cells that reacted with antisera directed towards either IgG, IgM, IgA or a T lymphocyte marker (MUII). A conspicuous feature was the prominent hyphal fluorescence seen with IgA and C3 antisera. The IgA reagent also marked large numbers of mononuclear cells both around lesions and scattered throughout interstitial tissue, suggesting an abnormality of IgA production or regulation as a factor predisposing to this condition. 相似文献
8.
Sosa ER Landsberg JH Stephenson CM Forstchen AB Vandersea MW Litaker RW 《Journal of aquatic animal health》2007,19(1):14-26
In the spring of 1998, the Florida Fish and Wildlife Research Institute received numerous reports of lesioned or ulcerated fish primarily from the St. Lucie Estuary on the southeast coast of Florida, an area known since the late 1970s for lesions of the ulcerative mycosis (UM) type. From these and archived reports, as well as others received from different areas of Florida, we documented that diseased specimens had randomly distributed skin ulcers (usually reddened or hemorrhagic) with raised irregular margins and, in some cases, deeply penetrating hyphae in the surrounding muscle tissue. Since 1998, 256 fish (comprising 18 species) with ulcerative lesions (from 15 different locations) were confirmed with hyphae in fresh squash preparation or by histological evaluation. Squash preparations revealed nonseptate, sparsely branching, thick-walled hyphae; histological sections revealed mycotic granulomas in the dermis that occasionally penetrated into the skeletal muscle. These pathological characteristics were consistent with UM caused by the oomycete Aphanomyces invadans in Southeast Asia, Japan, Australia, and the United States. For specific identification, six isolates from ulcerated fish were cultured and prepared for molecular characterization using established diagnostic methods. Ribosomal RNA gene sequence analysis identified three isolates as Aphanomyces invadans, one as the oomycete Achlya bisexualis, and two as the ascomycete Phialemonium dimorphosporum. A more extensive survey of 67 ulcerated skin samples from fish collected between 1998 and 2003 was performed using a polymerase chain reaction assay specific for Aphanomyces invadans. Of these, 26 (38.8%) samples from seven fish species and nine collection locations were positive. Confirmation of UM associated with Aphanomyces invadans represents new host records in Florida for the sheepshead Archosargus probatocephalus, striped mullet Mugil cephalus, white mullet Mugil curema, silver perch Bairdiella chrysoura, black drum Pogonias cromis, largemouth bass Micropterus salmoides, and American shad Alosa sapidissima. 相似文献
9.
An adult Bengal tiger (Panthera tigris tigris) housed in an outdoor sanctuary in Florida exhibited vomiting, diarrhea, and weight loss. A clinical workup did not reveal the source of the clinical signs and antibiotic therapy was unrewarding. Radiographs revealed the presence of an abdominal mass. The tiger died during an immobilization for a follow-up clinical examination. A necropsy was performed and tissue samples of intestine and mesenteric lymph nodes were submitted for histopathologic diagnosis. A pyogranulomatous panenteritis and lymphadenitis with intralesional hyphae led to a presumptive etiologic diagnosis of intestinal/abdominal pythiosis. The diagnosis of pythiosis was confirmed by serology and immunoblotting. 相似文献
10.
This case report describes the clinical and postmortem findings in a 2.5-year-old goat with necrotizing granulomatous pneumonia. The goat was referred to our clinic because of swelling of the head and neck, which was unresponsive to treatment, dysphagia, and deterioration in general condition. Thoracic radiographs showed two soft tissue densities, about 10 cm in diameter, in the left caudodorsal lung. The goat was euthanized and a necropsy was carried out. The two lesions in the left caudodorsal lung were round, firm and clearly demarcated from the surrounding lung tissue. They contained purulent material and compromised about 70 % of the diaphragmatic lung lobe. Histological examination of the lesions revealed a dense network of hyphae characteristic of Mucorales spp. 相似文献
11.
John T Mackie Arvind A Padhye Reginald J Sutherland Warwick A Lamb Stephen Davis 《Journal of veterinary diagnostic investigation》2004,16(3):248-250
This report describes severe generalized granulomatous lymphadenitis and splenitis in a 5-year-old, spayed female, Rottweiler dog with anorexia and diarrhea. There was replacement or effacement of much of the parenchyma of the lymph nodes and spleen by sheets of macrophages, multinucleated giant cells, and myriad nonpigmented fungal organisms, most of which appeared to be intracellular. These organisms were very pleomorphic, including large chlamydospore-like cells, small round yeast-like cells, and septate hyphae. A fungus identified as Monocillium indicum was isolated from lymph node tissue. To the authors' knowledge, this is the first report of infection with Monocillium in either humans or other animals. 相似文献
12.
J Bucek L Pospísil M Moster B Skalka 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1992,39(7):495-502
The histopathologic features of an experimental dermatophilosis of rabbit skin were described. The strain of Dermatophilus congolensis used for the experimental infection was obtained from the unique isolation and cultivation of this actinomycete from the ulcerous leg lesion of one male patient. Further strains in experiment came from the collections of type cultures. The experimental infection was characterized as an exudative and crustal dermatitis with acanthosis and subcorneal abscesses. Branching hyphae and clusters of coccoid bodies were found mostly in the parakeratotic layer and infrequently in the abscesses. Both the coccoid bodies and the hyphae were Gram-positive. The hyphae were acid-fast but the coccoid bodies were non-acid-fast. The fine structure of hyphae and coccoid bodies, namely of those with flagella, was also described by means of a transmission and scanning electron microscope. 相似文献
13.
红豆草黑腐病菌菌丝生长和产毒培养条件的优化 总被引：4，自引：2，他引：2
从红豆草自然发生的黑腐病病斑上分离得到细链格孢菌(Alternaria tenuis)菌株,对该病菌的菌丝生长及其产毒条件进行了研究。结果表明,病原菌在供试的6种培养液中均能生长并且产毒,但在PSK培养液中生长及产毒最好;在1-19d的培养时间内该病菌均可以生长及产生毒素,而在13 d时菌丝生长量及其产毒能力均达到最大,菌丝生长与产毒能力间显著正相关;该病原菌在5-40℃的范围内均可生长并产生毒素,但菌丝生长的最适温度为25℃,而最适合产毒的温度为15℃;在pH值为3~11的范围内,该病菌均能生长并产毒,而且偏酸性条件有利于菌丝的生长及其毒素的产生,菌丝生长和产毒的最佳pH值分别为4和6。 相似文献
14.
《Journal of aquatic animal health》2013,25(4):289-294
Abstract Vegetative hyphae of Saprolegnia parasitica, a common parasitic fungus offish and fish eggs, are aseptate and have thin walls (30–90 nm). The hyphae contain large vacuoles and have elongate mitochondria and nuclei oriented parallel to the longitudinal axis and in the direction of hyphal extension. Presporangium hyphae, those hyphae that will form sporangia, also are aseptate, but have thicker walls (120–170 nm) and contain smaller vacuoles. Presporangium hyphae do not, in general, have the longitudinal orientation of organelles seen in vegetative hyphae. Densebody vesicles and primary bars, or encystment vesicles, as well as mitochondria, dictyosomes, and endoplasmic reticulum, are consistent features of these presporangium hyphae. 相似文献
15.
木霉对草坪上2种重要土传病害生防效果的研究 总被引：8，自引：0，他引：8
利用木霉菌Trichoderma spp.防治草坪上2种重要的土传病害--褐斑病及镰刀枯萎病.木霉菌对草坪上的植物病原真菌室内抑菌效果及木霉制剂温室和田间防效试验结果表明,在拮抗作用测定中,木霉菌对草坪镰刀枯萎病菌和立枯丝核病菌具有较强的拮抗作用,拮抗机制主要为竞争作用、重寄生作用和抗生作用;温室盆栽及田间防治试验结果表明,木霉对镰刀枯萎病的防效分别为85%和74%,木霉对立枯丝核病菌的防效分别为86%和76%,且具有防效持久、刺激植物生长的作用. 相似文献
16.
Yasuda M Inoue T Takakura A Itoh T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(3):357-359
A 3-year-old female common marmoset was euthanatized because of wasting. Grossly, a perforating lesion was present in the jejunum and hemorrhagic lesions in the cecum and colon. Histopathologically, these gross lesions were a perforated jejunal ulcer and necrotizing colitis, respectively. Necrotizing colitis was characterized by extensive mucosal necrosis along with numerous ribbon-shaped aseptate hyphae. These aseptate hyaline hyphae were positively stained with PAS and GMS, and reacted immunohistochemically with the antibody against the family Mucoraceae. This case was diagnosed as intestinal mucormycosis. This is the first report on mucormycosis in a common marmoset. 相似文献
17.
Cutaneous pythiosis in beef calves 总被引：3，自引：0，他引：3
R I Miller B M Olcott M Archer 《Journal of the American Veterinary Medical Association》1985,186(9):984-986
Six Brahman and Brahman-cross calves less than or equal to 9 months old were examined because of ulcerative swellings of the fetlocks (5 calves) or numerous focal ulcerated cutaneous lesions (1 calf). Biopsies revealed focal cutaneous granulomas around regular, thick-walled branching hyphae, 4 to 9 micron in diameter. In all cases, portions of the hyphae were surrounded by granular encrustations, which ultrastructurally were composed of amorphous material comparable to antigen-antibody complexes. The protist Pythium sp was isolated from 2 calves. 相似文献
18.
John S Munday Richard A Laven Geoff M B Orbell Sushil K Pandey 《Journal of veterinary diagnostic investigation》2006,18(6):619-622
A 7-year-old dairy cow presented with clinical signs of neurologic disease. Despite treatment with penicillin, the cow died 36 hours after initial presentation. Necropsy examination revealed multiple foci of hemorrhage within the cerebrum and thickened meninges. Additionally, endometritis and consolidation of approximately 30% of both lungs was observed. Histology revealed necrotizing vasculitis, infarction, and hemorrhage within sections of the brain, uterus, and lung. Large numbers of intralesional fungal hyphae were visible. Because only formalin-fixed tissue was available, polymerase chain reaction was used to make an etiologic diagnosis of Mortierella wolfii. 相似文献
19.
Orós J Arencibia A Fernández L Jensen HE 《Veterinary journal (London, England : 1997)》2004,167(2):202-207
Post mortem examination of a juvenile loggerhead sea turtle (Caretta caretta) stranded in the Canary Islands revealed a fishing-line in the small intestine. Histologically, severe necrotic enteritis, multiple haemorrhages, and marked oedema of the intestinal submucosa were observed. Yeast cells and fungal hyphae were seen in the lamina propria of the intestinal mucosa and in the connective tissue of the submucosa. Because fungal cultures were not taken at the time of necropsy, an immunohistochemical study was performed in order to identify the fungus involved. Specific monoclonal and heterologously absorbed polyclonal antibodies served as the primary reagents for identification of aspergillosis, candidiasis, fusariosis, geotricosis, scedosporiosis, and zygomycosis, using an indirect immunofluorescence staining technique. The fungal elements were strongly stained only by a polyclonal antibody against Candida albicans and a monoclonal antibody against C. albicans. There are no known previous reports of Candida sp. causing skin disease or systemic mycotic infection in sea turtles. 相似文献
20.
Epichloё yangzii是共生于鹅观草属Roegneria植物的产子座有性型内生真菌。2004-2008年,分别在南京的不同地区采集产子座的鹅观草植株,研究E. yangzii在宿主植物体内的分布特征和种传能力。结果显示,E. yangzii在宿主的地上部分有系统的分布,并进入种子,可以进行垂直传播。同时发现种传的Epichloё属真菌在宿主植物体上也同样具有形成子座的能力,但子座不一定每年都能形成。在须根中没有发现E. yangzii的存在。菌丝体随在植株体内分布部位的不同,其形态特征有一定的差异。 相似文献