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1.
Silage making practices in respect of 130 samples of autumn made grass silages ensiled in bunkers on commercial farms in South Wales during 1983-1985 were analysed to discern the effect of wilting and/or silage additives on fermentation. Silages were primarily made during late September and early October in fine weather from perennial ryegrass pasture which had not been grazed for 6 weeks. On average 13.9 ha of pasture was cut for silage. Analysis of 120 samples of grass showed it contained 176 g kg?1 dry matter (DM) with (g kg?1 DM) 215 protein, 240 modified acid detergent fibre (MADF), 78 water-soluble carbohydrate (WSC) and 95 ash. Most farmers attempted to pre-wilt grass for 1 day prior to ensilage and one quarter of them made unwilted silage. Silage making was usually completed within 6 days of starting to cut and was generally made with a precision-chop machine. Silage additives applied were (kg t?1) formic acid (4·7), formic acid 4-formalin (5·5), sulphuric acid-+ formalin (5·0) sugars (14) and inoculants (0·65). Formic acid significantly reduced pH, and formic acid with or without formalin significantly reduced ammonia nitrogen (N) content of silages compared with other treatments. Protein contents of acid/formalin treated silages were significantly higher and MADF of acid with or without formalin treated silages were significantly lower than other treatments. Pre-wilting grass prior to ensilage did not significantly increase subsequent silage DM content and significantly increased the pH of non-additive treated silages. Unwilted silages treated with formic acid with or without formalin had a significantly lower ammonia-N content and higher residual WSC than other treatments. It is suggested that only formic acid application either alone or in conjunction with formalin to unwilted silage was successful in producing well preserved silages and that a grass WSC content of 17 g kg?1 would be necessary to achieve this.  相似文献   

2.
An experiment was carried out during 1984 to study the effect of treating grass at ensiling with three commercially available inoculant-type additives (H/M Inoculant, Grass Sile and Siron), formic acid (850 g kg−1; Add-F) or no additive on grass preservation, in-silo loss, intake and animal performance. Primary growth grass ensiled from 28–29 May into concrete-walled covered silos was of high dry matter (DM, 234 g kg−1), water-soluble carbohydrate content (WSC. 212 g kg DM−1) and digestibility (MADF, 250 g kg DM−1).
The untreated silage displayed good preservation and with the exception of the Sirontreated silage which showed significantly lower buffering capacity (Be) and volatile fatty acid (VFA) contents than the untreated silage, the application of inoculant-type additives did not improve silage preservation or decrease in-silo DM losses. The formic acid-treated silage displayed significantly lower Be, water-soluble carbohydrate, ash, ammonia nitrogen (g kg total N−1) and lactate contents than the untreated silage.
After a 133 d storage period, silages were offered to finishing beef cattle for an 84-d period. Cattle offered the silages displayed similar and non-significant daily DM intakes, daily liveweight gains, dressing proportions and daily carcass gains. From this experiment it appears unlikely that any of the additives evaluated will improve animal performance relative to a well-preserved untreated silage.  相似文献   

3.
A randomized block experiment involving thirty-six lactating dairy cattle was carried out to evaluate a bacterial inoculant (Grazyme Grobac, Agritech), containing bacteria, enzymes and a rumen enhancer, as a silage additive. Herbage from the primary regrowth of predominantly perennial ryegrass swards was ensiled unwilted and precision-chopped after a 51-d growth interval on 4 and 5 August. Alternate loads of herbage were ensiled either untreated (C) or treated with formic acid at a rate of 2.65 1 (t herbage)?1 (F) or the inoculant at a rate of 0.64 kg (t grass)?1 (I). The treatments were harvested using the same harvester in the rotation of I, F and C treatments. Mean dry-matter (DM), water-soluble carbohydrate and nitrate nitrogen (N) concentrations and buffering capacity of the C herbages at ensiling were 198 g kg?1, 18 2 g kg?1, 290 mg (1 juice)?1 and 379 mequiv. (kg DM)?1 respectively. For silages C, F and I pH values were 3.7, 3.8 and 3.8 and ammonia N concentrations 61, 43 and 58 g (kg total N)?1 respectively. Inoculant treatment did not alter aerobic stability of the silages, whereas formic acid treatment increased it. The silages were offered ad libitum and supplemented with 5 kg of concentrates per head daily. For treatments C, F and I silage DM intakes were 10.8, 11.2 and 10.8 (s.e. 0–33) kg d?1, milk yields 21.3, 20.9 and 20.7 (s.e. 0.52) kg d?1, fat concentrations 38.3, 40.3 and 37.2 (s.e. 0.83) g kg?1 and protein concentrations 30.8, 32.6 and 32.6 (s.e. 0.49) g kg?1 respectively. Inoculant treatment did not alter (P0.05) the digestibility coefficients of the total diets, whereas formic acid treatment decreased DM (P0.05), organic matter (P0.05), neutral detergent fibre (P0.01) and hemicellulose (P0.01) digestibilities. Formic acid treatment altered rumen fermentation patterns, whereas inoculant treatment had no effect. It is concluded that, relative to a well-preserved untreated silage, additive treatment did not alter DM intake, milk yield or fat plus protein yield. However, both additive treatments increased milk protein concentrations and formic acid treatment increased milk fat concentration relative to the well-preserved, untreated silage.  相似文献   

4.
The effects of offering a range of grass silages and mixtures of grass and maize silages on the intake of beef cattle were studied. Four grass silages (GS1, GS2, GS3 and GS4) were used. Grass silage 1 was ensiled from a second regrowth in mid‐late September and treated with an inoculant additive. Grass silages 2, 3 and 4 were ensiled, without additive, from a primary regrowth harvested in early July, late May and mid‐June respectively. Wilting periods were 8, 30, 36 and 36 h for GS1, GS2, GS3 and GS4 respectively. Grass silages 1, 2 and 3 were precision chopped and ensiled in bunker silos, while GS4 was ensiled in round bales. The DM content (g kg?1) and starch concentration (g kg?1 DM) of the three maize silages (MS1, MS2 and MS3) used were 256 and 128, 256 and 184, and 402 and 328 for MS1, MS2 and MS3 respectively. Seventy‐two Charolais and Limousin cross‐bred steers were used in a changeover design with two 4‐week periods. The study consisted of sixteen treatments incorporating the four grass silages fed alone and with the three maize silages arranged as a 4 × 4 factorial design. The grass silage and maize silage mixtures were offered in a ratio of 0·60:0·40 (DM basis) once daily using individual Calan gates. All silages were offered ad libitum with 3 kg per head per day of a concentrate supplement. Dry matter and metabolizable energy (ME) intakes were highest with diets based on grass silage GS4 compared with diets containing the other grass silages. Metabolizable energy intakes of diets containing no maize silage, and those based on MS1 and MS2, were similar (P > 0·05) but lower than that of diets containing MS3. Only limited increases were found in DM and ME intakes with the inclusion of maize silage in grass silage‐based diets while offering high‐quality grass silage (assessed in terms of DM content, and fibre and N concentrations) promoted high voluntary intakes.  相似文献   

5.
An experiment is described in which three silages were prepared from herbage treated with either a bacterial inoculant (Ecosyl, Imperial Chemical Industries plc) at 2-71 t?1, formic acid (850 g kg?1, Add-F, BP Chemicals International Ltd.) at 2-21 t?1, or no additive (control). The herbage (second regrowth from perennial ryegrass swards), was ensiled unwilted, and had mean dry matter (DM) and water-soluble carbohydrate concentrations at ensiling of 186 and 34-1 g kg?1 respectively. The resulting silages were all well preserved and had only minor chemical differences. Thirty lactating British Friesian cows were subjected to a 21 d standardization period and were then offered the three silages for 21 d in a randomized-block design experiment. The animals were stalled individually, offered the silages ad libitum and in addition received 5 kg d?1 of a supplement containing 198 g crude protein kg DM?1. The mean silage DM intake, milk yields and milk fat concentrations during the final 7 d on treatment were 8-44, 8-62 and 887 kg d?1; 238, 230 and 25-1 kg d?1; and 368, 400 and 366 g kg?1 for the control, formic acid- and inoculant-treated silages respectively. Following the feeding trial, six cows per treatment were subdivided to provide three animals which were offered silage alone, and three offered silage plus 5 kg d-’supplement. Rumen samples taken after 21 d on these treatments indicated no effect of the inoculant on ruman volatile fatty acid proportions, whereas the use of formic acid resulted in increases in both acetate and butyrate concentrations. The results from this study support that of the earlier work that this particular inoculant, when used at ensiling, can considerably improve animal performance over that achieved with both untreated and formic acid-treated silages.  相似文献   

6.
Eight grass silages were made from two contrasting varieties of perennial ryegrass, four silages each from a late-cut early-maturing (high dry matter, HDM) variety and from an early-cut, late-maturing (low dry matter, LDM) variety. The grass was ensiled without additive (untreated), or with formic acid, or with one of two enzyme mixtures of hemicellulases and cellulases (enzyme 1 and enzyme 2) for a period of 130 d.
Formic acid-treated silage had lower levels of lactic acid at both levels of dry matter than the other silages. Enzyme treatment of grass prior to ensilage resulted in reduced levels of cellulose, acid-detergent fibre and neutral-detergent fibre in LDM silages and lower acid-detergent fibre and neutral-detergent fibre in the HDM silages compared with the corresponding untreated and formic acid-treated silages. Voluntary intakes (g DM d−1) of untreated and enzyme-treated silages were significantly ( P <0·01) lower at both digestibilities compared with formic acid-treated silages (LDM: untreated, 982; formic, 1069; enzyme 1, 868; enzyme 2, 937; HDM: untreated, 931; formic, 1027; enzyme 1, 943; enzyme 2, 914). The organic matter, carbohydrate and nitrogen digestibility coefficients of LDM silages were significantly ( P <0·001) higher than those of HDM silages. There were no significant differences in any component digestibility related to silage additive.
Comparison of digestibility coefficients for constituents of the LDM silages fed to sheep or steers showed no differences between species.  相似文献   

7.
Herbage from the first regrowth of perennial ryegrass-based swards was directly ensiled after treatment with a bacterial inoculant/enzyme preparation (SIL-ALL, Alltech UK) at 3·0 1 t?1, formic acid (850 g kg?1) at 2·59 1 t?1 or no additive (Control). The mean dry matter (DM) and water-soluble carbohydrate concentrations of the grass were 185 and 24·0 g kg?1 (fresh basis) respectively. Lactic acid concentrations after ensiling increased at a lower rate in formic acid-treated herbage than with the other treatments. All silages were well preserved and formic acid-treated silage had a lower ultimate concentration of lactic acid and higher concentration of water-soluble carbohydrate. Effluent output was increased on a proportional basis by ?0·06 with formic treatment, whereas the inoculant reduced effluent output by 0·05 in comparison with the mean effluent production of the control silage. The in vivo digestibilities of the silages were determined using sheep. The digestibilities of DM, organic matter and energy were significantly higher with inoculant-treated silage than with formic acid treatment, whereas values for the control silage were intermediate. The three silages were offered ad libitum to forty dairy cows with individual recording of daily intakes for a 10-week period in a randomized block experiment with four treatments. Sixteen animals were offered the control silage with half of these offered 3 kg concentrates per day (C3) and the other half offered 7 kg concentrates per day (C7). Twelve animals were allocated to each of the additive-treated silages, with concentrates offered at 5 kg d?1. Treatment effects on animal performance were measured in weeks 7–10. To compare animal performance for the treated silages with the control, an estimate of performance at 5 kg concentrates per day was obtained by regression using values obtained at 3 and 7 kg concentrates. In comparison with estimated silage intake for the control silage with 5 kg d?1 concentrates, inoculant and formic acid treatment of the silages increased dry matter intake by 0·04 (P > 0·05) and 0·13 (P > 0·01) respectively. In comparison with estimated milk production and yield of fat plus protein for the control treatment with 5 kg d?1 concentrates, neither inoculant treatment nor formic acid treatment produced any significant differences.  相似文献   

8.
First-harvest direct-cut, double-chopped grass (190 and 164g DMkg?1 in Experiments 1 and 2 resptectively) was ensiled without an additive or, in Experiment 1, with 30 kg t?1 grass of an absorbent additive based on sugar beet pulp (Sweet ‘n’ Dry) or with 3·441 t?1 grass of formic acid and, in Experiment 2, with 30, 50 and 70 kg t?1 grass of Sweet ‘n’ Dry or with 50kg t?1 grass of unmolassed sugar beet pulp. The preservation and nutritive value of the silage, in-silo losses (including silage effluent production), silage intake and animal performance of adult and growing cattle were examined. In Experiment 1 all three silages were well preserved, although the formic acid-treated silage displayed significantly lower pH, ammonia nitrogen (NH3N) [g kg?1 total nitrogen (TN)] and volatile fatty acids (VFAs) than the other two silages. In Experiment 2 absorbent-treated silages displayed significantly lower pH, buffer capacity (Bc), NH3N (gkg?1 TN), CP, modified acid detergent fibre (MADF) and VFAs than untreated silage. Treatment of grass with the absorbent additives at ensiling resulted in reduced effluent production. In Experiment 1 each kilogram of Sweet ‘n’ Dry retained approximately 11 effluent, and in Experiment 2 silages made with Sweet ‘n’ Dry applied at 70kgt?1 and sugar beet pulp applied at 50 kg t?1 produced similar volumes of effluent and each kilogram of absorbent retained 1·0 and 1·31 of effluent respectively. In Experiment 1 sixty beef cattle [mean initial live weight (LW) 460 kg] were grouped according to LW and allocated to treatment at random. For untreated silage (unsupplemented or with 1 or 2 kg supplement head?1 day?1), absorbent-treated silage (unsupplemented or with 1 or 2 kg supplement head?1 day?1) and formic acid-treated silage (1 kg supplement head?1 day?1) the daily silage DM intakes were 6·12, 6·21, 6·40, 7·65, 7·45, 7·11 and 7·85 (s.e. 0·280) kg respectively, the daily liveweight gains were 0·22, 0·56, 0·81, 0·59, 0·74, 0·81 and 0·75 (s.e. 0·071) kg respectively and daily carcass gains were 0·31, 0·47, 0·67, 0·47, 0·61, 0·70 and 0·57 (s.e. 0·043) kg respectively throughout a 75-day feeding period. In Experiment 2, fifty-six growing cattle (mean initial weight 312 kg) were grouped according to LW and allocated to treatment at random. For untreated silage (unsupplemented or with 1·5 kg Sweet ‘n’ Dry or 1·5 kg commercial concentrates head?1 day?1), silage treated with Sweet ‘n’ Dry at 30, 50 and 70 kg t?1 grass and silage treated with 50kg sugar beet pulp t?1 grass the daily silage DM intakes were 5·46, 5·28, 5·33, 6·21, 6·27, 6·60 and 6·62 (s.e. 0·154) kg respectively and daily liveweight gains were 0·39, 0·75, 0·81, 0·63, 0·76, 0·94 and 1·75 (s.e. 0·052) kg respectively throughout a 122-day feeding period. In this experiment 360g kg?1 more absorbent was required when it was included at ensiling rather than offered as a supplement to untreated silage to achieve the same individual animal performance.  相似文献   

9.
Data from twenty experiments, conducted at ADAS Research Centres in England and Wales during 1986–92, were used to determine effluent production from additive-treated grass silages made in large-scale bunker silos. The additives compared were formic acid at 4·0 l t–1, rolled barley at 44·0 kg t–1, dried molassed sugar beet feed at 40·0 kg t–1 and liquid inoculants at 2·2 l t–1 together with a non-additive-treated control. The silages were made from herbage with an average dry-matter (DM) content of 177 (s.e. 3·8) g kg–1 and water-soluble carbohydrate content of 140 g kg–1 DM. Average silage toluene DM content was 213 (s.e. 3·8) g kg–1. The formic acid and sugar beet feed silages were both well fermented, whereas the other silages were less well fermented. Effluent produced was determined as either effluent production (l t–1 grass ensiled) during the 52-d period in which it was measured or peak flow (l h–1) during the first 2 d of ensilage. Compared with non-additive-treated silage, dried molassed sugar beet feed significantly reduced both effluent production (27%) and peak flow (36%). Formic acid significantly increased peak flow (51%), but had little effect upon effluent production, and significantly reduced effluent N and lactic acid content. Barley and inoculant treatment had no significant effect upon effluent production. In general, poor relationships were found between DM content and effluent production. Nevertheless for silages, except those treated with absorbents or formic acid, a significant (P < 0·001) negative relationship between silage effluent production (l t–1 grass ensiled) and the DM (g kg–1) content of the ensiled grass was found.  相似文献   

10.
Two silages were made from perennial ryegrass ensiled without wilting in 2-t capacity silos with the application of either formic acid or an enzyme mixture of cellulases and hemicellulases. Effluent losses were monitored over the ensiling period. Subsequent silage analysis showed that the enzyme-treated silage had higher concentrations of residual water soluble carbohydrate, lactic acid and acetic acid, and lower concentrations of cellulose, ADF and NDF. Effluent production was higher with the enzyme silage (formic acid, 211 1 t?1; enzyme, 2671 t?1). The silages were either offered as the sole diet or supplemented with rapeseed meal at two levels (60 or 120 g fresh weight kg?1 silage DM offered) to growing steers equipped with rumen cannulae and T-piece duodenal cannulae. Apparent whole tract digestibilities for DM, OM, N, ADF and NDF were similar for all diets although nitrogen retention (g d?1) was increased with supplementation of both silages (formic acid, 21·1; formic acid + 60 g, 23·5; formic acid+ 120 g, 28·5; enzyme, 22·6; enzyme + 60 g, 25·8; enzyme+ 120 g, 31·6). Rumen pH, ammonia and total volatile fatty acids patterns were similar. Supplementation increased the amount of organic matter apparently digested in the rumen (ADOMR) with formic acid-treated silage but not with enzyme-treated silage. Liveweight gains were similar for both unsupplemented silages (0·49 kg d?1). These increased to 0·55 and 0·65 kg d?1 for formic + 60 and formic + 120 respectively. Liveweight gains for the corresponding enzyme-treated supplemented diets were 0·81 and 0·91 kg d?1 respectively. Liveweight gains on supplemented enzyme-treated diets were significantly (P < 0·05) greater than those on formic acid-treated diets.  相似文献   

11.
A first cut of timothy, treated with water (untreated), formic acid (FA), cellulase + lactic acid bacteria (CB), cellulase + hemicellulase (CH) or cellulase + hemicellulase + a lignin-modifying enzyme (CHL), was ensiled in pilot-scale silos. Silages, except CB, were fed to four male cattle, each equipped with a rumen and duodenal cannula, in a digestibility trial designed as a 4 × 4 Latin square. The animals were fed a diet of 400 g of concentrate and 600 g of silage at a level of 70 g DM kg?1 live weight (LW0·75). All enzyme-treated silages were well-preserved with a more extensive fermentation than in FA silage. The quality of untreated silage was poorer as indicated by higher pH and ammonia-N content. The amount of effluent from enzyme-treated silages ranged from 116 to 127 g kg?1; for FA and untreated silages values were 101 g kg?1 and 80 g kg?1, respectively. Total DM losses from enzyme-treated silages were higher than from FA silage (P < 0·05). No significant differences were noticed between silages in the apparent digestibility of organic matter (OM), neutral-detergent fibre (NDF), acid-detergent fibre (ADF) or nitrogen (N). The apparent digestibility of cellulose was higher with enzyme-treated silages than with FA silage (P < 0·05). The values for microbial N flow at the duodenum were 80·0, 91·9, 80·7 and 70·5g N d?1, and for the efficiency of rumen microbial N synthesis 38·6, 47·6, 36·9 and 32·5 g N kg?1 OM apparently digested in the rumen for untreated, FA, CH and CHL silages, respectively. In the rumen the molar proportion of propionate was higher (P < 0·01) and that of butyrate lower (P < 0·01) with enzyme-treated silages when compared with FA silage. The proportion of butyrate was also lower with untreated than with other silages (P < 0·01). The rumen residence time of NDF and ADF was longer (P < 0·05) with enzyme-treated silages than with FA silage.  相似文献   

12.
Three silages were prepared from herbage treated with either an inoculant (Ecosyl, Imperial Chemical Industries plc) at 3.21 t?1, formic acid (850 g kg?1, Add F BP Chemicals International Ltd) at 2·3 1 t?1, or no additive (control). The herbage used was the first regrowth from perennial ryegrass swards. It was ensiled unwilted, and had mean dry matter and water soluble carbohydrate concentrations at ensiling of 154 and 24·1 g kg?1 respectively. Time course studies showed only minor effects of additive treatment on fermentation patterns within the silo and all three silages had good fermentations. Over an 88 d feeding period, commencing on day 7 of lactation, forty-eight British Friesian cows were used to evaluate the silages in a three-treatment, randomized-block design experiment. The animals were stalled individually, offered the silages ad libitum, and in addition received 5 kg d?1 of a supplement containing 196 g kg?1 crude protein. On the basis of the data recorded during the final 28 d on treatment the animals receiving the inoculant-treated silage consumed 12 and 10% more silage dry matter and produced 2·1 and 2·3 kg d?1 more milk than those given the control and formic acid-treated silages respectively. Over the total experimental period the milk yields were 1957, 1894 and 2094 (±41·3) kg for animals receiving the control, formic acid- and inoculant-treated silages respectively. Animals offered the formic acid treated silage produced milk of significantly higher fat concentration than those given the other two silages. Total ration digestibility studies, conducted with three cows per treatment, indicated no significant differences in digestibility coefficients, nitrogen utilization or metabolizable energy concentrations of the three treatment diets. It is concluded that the higher milk yield recorded with the inoculant-treated silage, and the higher milk fat concentration with the formic acid-treated silage, over that obtained with the control silage, were due to the increases in ME intake of 5 and 16 MJ d?1 for the formic acid and inoculant-treated silages respectively.  相似文献   

13.
A total of 1713 samples of silage from commercial farm silos were analysed to investigate the effect of dry matter (DM) content and chemical additives on fermentation as measured by ammonia-N concentration and pH, Increasing DM content without additive use had a major beneficial influence upon fermentation. When silage DM contents were greater than 260 g kg-1 83% of silages were well fermented, with average ammonia-N concentrations of 94 g (kg total N)-1 and pH 4.36. With diminishing DM concentration the proportion of well fermented silages declined. In the DM range 220-260 g kg-1 67% of silages were well fermented with ammonia-N concentrations of 125 g (kg total N)-1 and pH 4.30, in the DM range 180-220 g kg-1 48% were well fermented with ammonia-N at 151 g (kg total N)-1 and pH 4.38 and with DM below 180 g kg-1 no silages were well fermented with ammonia-N concentration of 252 g (kg total N)-1 and pH 4.84. The benefit of chemical additives, albeit at poorly defined and often inadequate rates, was small in comparison to that of increased DM concentration. Below DM concentrations of 180 and 220 g kg-1, the only benefit was that formic acid decreased ammonia-N to 151 g (kg total N)-1 and pH to 4.32 compared with 163 g (kg total N)-1 and pH 4.43 for untreated silages. Within the DM range 220-260 g kg-1 formic acid decreased ammonia-N level to 104 g (kg total N)-1 and pH to 4.07, and there was a slight benefit from using sulphuric acid/formalin which decreased ammonia-N to 117 g (kg total N)-1 and pH to 4.23 compared with 125 g (kg total N)-1 and pH 4.27 for untreated silages. Above 260 g DM kg-1 both formic acid and sulphuric acid/formalin provided a small but consistent decrease in ammonia-N and pH compared with untreated silages. Chemical additive use conferred no other benefit when compared with untreated silage. Calcium formate/sodium nitrite mixtures and acid mixture use provided no benefit in fermentation compared with untreated herbage.  相似文献   

14.
Pure perennial ryegrass or perennial ryegrass/white clover mixtures (70:30 and 40:60 on a fresh-matter basis) were ensiled in laboratory silos either untreated or alter treatment with freshly cultured Lactobacillus (Lb.) plantarun or freshly cultured Lb. plantarum plus Lactococcus (Lc.) lactis. freeze-dried Lb. plantarum or freeze-dried Lb. Plantarum plus sodium formate, sodium formate or formic acid. The effect of these additives on silage fermentation characteristics and quality of the resultant silages was examined. There were significant interactions between treatments and herbages for all silage quality parameters measured, except for acetic acid concentration. The influence of additives on the final pH of all silages was small but statistically significant. Lactic acid concentration was not directly related to herbage mixture, overall mean values ranging from 118 to 120 ± 1.5 g kg?1 dry matter (DM), but wider variation was seen between treatments for individual herbage mixtures. Acetic acid concentrations were significantly (P<0·001) affected by herbage mixture ensiled, increasing linearly as clover content increased from zero to 60%. Untreated control and formic acid-treated silages contained significantly (p<0·001) higher acetic acid concentrations than those treated with other additives. Silage ammonia N concentrations were significantly (p<0 001) influenced by herbage mixture. Lowest ammonia N concentrations (< 50 g kg?1 DM) were observed in silages that had been treated with formic acid, freshly cultured Lb. plantarum or Lb. plantarum plus Lc. lactis. The fraction 1 leaf protein (FILP) contents of silages were significantly (P <0·001) affected by both treatment and herbage mixture, with consistently and significantly higher values found in freshly cultured inoculant-treated silages. A poor correlation (r2= 0·12) existed between ammonia N and FILP in all silages. The inclusion of up to 60% white clover in the ensiled herbage did not adversely affect final silage quality. However, additive treatment markedly influenced the residual FILP content of silages, those treated with freshly cultured inoculants having the highest values.  相似文献   

15.
Four grass silages were made from perennial ryegrass ensiled after a 1h wilt in 2-t silos without additive application, with application of formic acid or with one of two enzyme mixtures of hemicellulases and cellulases (enzyme 1 and enzyme 2). Effluent losses were monitored over the ensiling period (130 d).
Analyses of the silage showed that formic acid-treated silage had lower concentrations of lactic acid than the other silages. Both enzyme-treated silages had lower levels of cellulose, acid detergent fibre (ADF) and neutral-detergent fibre (NDF) than the untreated and formic acid treated silages. Effluent production was highest with enzyme-treated silages.
The silages were subsequently fed to growing steers equipped with rumen cannulae and T-piece duodenal cannulae. Apparent whole-tract digestibilities of organic matter constituents were significantly lower ( P < 0·05) with both enzyme-treated silages (untreated; 0·736, formic acid; 0·722, enzyme 1; 0·694, enzyme 2; 0·703). Both untreated and enzyme 2-treated silages sustained higher nitrogen digestibilities (g g−1 intake) (untreated; 0·675, formic acid; 0·636, enzyme 1; 0·630, enzyme 2; 0·662) and N retentions (g d−1) untreated; 16·0, formic acid; 14·0, enzyme 1; 11·6, enzyme 2; 16·6), but none of these differences was significant. When formic acid-treated silage was offered, there was a greater amount of organic matter apparently digested in the rumen (ADOMR). Non-ammonia nitrogen and microbial nitrogen flows at the duodenum were similar on all diets. The efficiency of microbial protein synthesis was highest with enzyme 2-treated silage and lowest with formic acid-treated silage (untreated, 35·4; formic acid, 25·2; enzyme 1, 30·4; enzyme 2, 39·4), but none of these differences were significant.  相似文献   

16.
The aim of the experiment was to assess the effect of applying, at commercially recommended rates, formic acid or formic acid/formaldehyde at ensilage upon the subsequent digestion of the silages by cattle. Three wilted grass silages were made from perennial ryegrass ensiled at a DM concentration of 206 g kg−1 after a poor wilting period of 49 h without additive application and with application of 2.5 litres t−1 of formic acid or of 4.5 litres t−1 of a mixture of (gkg−1) 500 formic acid, 200 sulphuric acid and 200 formatin giving an application rate of 15 g formaldehyde kg−1 herbage crude protein (N × 6.25).
The silages were fed to cattle equipped with rumen cannulae and duodenal re-entrant cannulae. Results from analyses of silage composition and from the digestion of organic matter and N showed no major differences between silages. The efficiency of rumen microbial N synthesis, the rumen degradability of silage N (determined in vivo or in sacco ) and in vivo digestion of individual amino acids were also unaffected by additive treatment. These results indicate that poor wilting conditions before ensilage restricted the effectiveness of additive treatment; the ineffectiveness of formaldehyde in reducing the rumen degradability of silage N may also have been related to the low rate of formaldehyde application.  相似文献   

17.
Two experiments were carried out with grass silages cut at a leafy (Experiment 1) and a more mature (Experiment 2) stage of growth to evaluate the effect of wilting and chop length on silage intake and performance of store lambs. In each experiment, the herbage was cut with a rotary mower and was either ensiled within 24 h as unwilled silage (U) or wilted for 1–3 d (W). Each silage type was harvested with either a double-chop harvester (D) or a precision-chop harvester (P). All silages were treated with formic acid at 3 1 t?1 and were well preserved. The silages were fed ad libitum to Suffolk crossbred store lambs (twenty-four lambs per treatment) without any supplement over a period of 8 or 9 weeks. Wilting of the silages had little effect on silage intake (797 vs. 809g dry matter (DM) d?1) or on lamb performance in Experiment 1. In Experiment 2, wilting of the D silage increased silage DM intake (589 vs. 534 g DM d?1; +10%) and reduced the extent of liveweight losses. Wilting of the P silage reduced silage intake (770 vs. 791g DM d?1; -3%) and reduced liveweight gains. In Experiment 1 intakes of the D silages were 650–667g DM d?1 and just maintained lamb live weights. Intakes of the P silages were 39–49% higher than the D silages (927–968 g DM d?1) and increased liveweight gains. In Experiment 2 intakes of the D silages were 534–589 g DM d?1 and resulted in a loss in lamb live weight. Precision-chopping increased silage intakes by 31–48% (770–791 DM d?1)in Experiment 2 and improved lamb liveweight gains. Lamb performance was higher on the UP silage than on the WP silage. The rumen retention lime (RRT), estimated from the rumen contents of the lambs at slaughter and their silage intake before slaughter, was much shorter for the lambs fed on the P silages (12.6–20.6 h) than those fed on the D silages (21.4–29.3 h) in each experiment. Silage intake and liveweight gain were positively related to silage in vivo DM digestibility (DMD), whereas RRT was negatively related to DMD. However, there were distinct differences between the P and D silages in the elevation and, to a lesser extent, in the slope of the regression lines, indicating that intake of D silage was limited by factors other than the digestibility of the silage The results of this study show that the chop length of grass silage had a far greater effect on intake and on lamb performance than silage digestibility, whereas wilting had little or no effect.  相似文献   

18.
Fonr grass sUages made in May from the same herbage were compared io a 16-week feeding experiment with 12 Ayrshire cows. The silages were made from herhage wilted for either 1 or 2 days, and with or without 1/2 gal formic acid per ton of herhage. The silages treated with acid were, on average, 5°C cooler than the untreated silages, had lower pH values, higher lactic acid contents, slightly higher DM and crude protein contents, and higher contents of digestible organic matter in the DM. The silages were fed ad lib. with a supplement of harley and groundnut cake. The intakes of silage DM were higher with the acid-treated than with the nntreated silage. The mean daily milk yields on the silages made with and without the acid additive were 35.4 and 33.1 lb (16.1 and 15.0 kg), respectively, for the silage, wilted for 1 day and 34.2 and 32.2 lb (15.5 and 14.6 kg) for the silage wilted for 2 days. The effects of the treatments on the composition of the milk were small. It is concluded that the silages treated with formic acid additive were superior to the nntreated silages as a feed for dairy cows.  相似文献   

19.
This experiment evaluated a bacterial inoculant based on a single strain of Lactobacillus plantarum as a silage additive. Three silages were harvested on 8 September 1989 from the second regrowth of a perennial ryegrass sward, which had received 167 kg N, 28 kg P2O5 and 45 kg K2O ha?1. Mean dry matter (DM) and water soluble carbohydrate concentrations of the herbages at ensiling were 148 g kg?1 and 78 g.(kg DM)?1 respectively. Herbages were treated with either no additive (C), formic acid (3·0 1 t?1) (F) or the inoculant (3·0 1 t?1) (I) and were ensiled in three 80-t capacity silos. For silages C, F and I respectively, pH values were 4·70, 3·77 and 4·47, ammonia-N concentrations were 192, 111 and 182 g (kg total N)?1 and butyrate concentrations were 6·8, 1·8 and 7·1 g (kg DM)?1. The silages were offered ad libitum and supplemented with 2·0 kg concentrates per head daily to thirty-six heifers (mean initial live weight 442 kg). For silages C, F and I, silage DM intakes were 12·7, 14·4 and 14·1 (s.e. 0·42) g (kg live weight)?1, metabolizable energy intakes were 155, 166 and 172 (s.e. 5·1) kJ (kg live weight)?1, and estimated carcass gains were 456, 519 and 518 (s.e. 28·1) g d?1 respectively. A further 18 similar cattle were used in studies on the digestibility of the silages, and rumen degradation of each was estimated with three mature cattle. Inoculant treatment significantly increased crude fibre (P <0·01), neutral detergent fibre (P <0·01), modified acid detergent fibre (P <0·01), hemicellulose (P <0·05) and N digestibilities (P <0·05) and tended to increase N retention from the total diet. It is concluded that although treatment of herbage that was difficult to ensile with the inoculant did not improve silage fermentation, it significantly increased digestibility, especially of the fibre fractions, and silage DM intake and tended to increase animal performance to levels similar to those achieved with a well preserved formic acid-treated silage. Increases in silage DM and metabolizable energy intakes are likely to have been attributable to the effects of the inoculant on digestibility, especially of the fibre fractions.  相似文献   

20.
An experiment was carried out to examine silage fermentation, effluent production and aerobic stability in unwilted grass silage, which was either ensiled without additive or with a commercially available blend of ammonium hexamethanoate, ammonium hexapropionate and octanoic acid (6 l t?1, Maxgrass, BP Chemicals Ltd., Northwich, UK) and to determine alternative approaches to obtaining the same performance in finishing beef cattle. Seventy‐two Limousin × Friesian and Charolais × Friesian steers (mean initial live weight 424 kg s.d. 28·3) were blocked into groups of nine according to live weight and previous performance and offered silage, either with or without the additive, and supplemented with 0, 1·5, 3·0 or 4·5 kg d?1 of a concentrate with a crude protein content of 150 g kg?1 DM or allocated to a pre‐experimental slaughter group to enable calculation of daily carcass gain. Daily silage intakes were recorded for 112 days. At the end of the experiment, all cattle were slaughtered and daily carcass gain, omental, mesenteric, perinephric and retroperitoneal fat depots [kidney‐knob and channel fat (KKCF)], fatness, conformation, subcutaneous fat depth over M. longissimus dorsi muscle and carcass fat, protein and bone contents were assessed. Across all levels of supplementation, cattle offered the silage with the additive showed significantly (P < 0·001) higher daily DM intakes than those offered the silage without additive. Cattle offered the silage with the additive but unsupplemented had significantly (P < 0·001) higher daily carcass gains than those offered the silage without additive and unsupplemented. The response in carcass gain was 76 and 35 g kg?1 additional concentrate for the silages with and without the additive respectively.  相似文献   

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