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1.
Summary Cytogenetic evidence has shown that teosinte (Zea mays ssp. mexicana (Schrad.) Iltes) and maize (Zea mays L. ssp. mays) are conspecific. They hybridize readily and their offspring are generally fertile. Teosinte could not have originated as a byproduct of maize-Tripsacum hybridization. Such introgression gave rise to plants that are phenotypically maize or Tripsacum, depending on which parent was used as a pollen donor. Compartive morphological and genetical studies indicated that it is more probable that maize originated from a teosinte-like ancestor under domestication, than that a maize-like plant gave rise to teosinte through a series of mutations.Reseach supported financially by the Illinois Agricultural Experiment Station, and Grants GB-40136-X and BM573-01034 A02 from the National Science Foundation.  相似文献   

2.
Y. Mano    M. Muraki    M. Fujimori    T. Takamizo    B. Kindiger 《Plant Breeding》2005,124(5):432-439
Two genetic linkage maps of Zea mays were constructed: one population comprised 94 F2 individuals of a dent ‘B64’ × teosinte (Z. mays ssp. huehuetenangensis) cross while the second consisted of 94 F2 individuals of a ‘B64’ × Caribbean flint ‘Na4’ cross. The level of polymorphism was higher in the ‘B64’ × teosinte combination than the ‘B64’ × ‘Na4’ combination. In the ‘B64’ × teosinte cross, a total of 338 amplified fragment length polymorphism (AFLP) and 75 simple sequence repeat (SSR) markers were mapped to 10 chromosomes, which covered 1402.4 cM. In the ‘B64’ × ‘Na4’ cross, a total of 340 AFLP and 97 SSR markers were mapped to 10 chromosomes, covering 1662.8 cM. Segregation distortion regions were found on chromosomes 4, 5 and 8 in the ‘B64’ × teosinte cross and on chromosome 9 in the ‘B64’ × ‘Na4’ cross. Comparison of the two maps revealed that the maize × teosinte map was 11.5% shorter than the maize × maize map. The maps generated in this study may be useful to identify genes controlling flooding tolerance.  相似文献   

3.
Zea mays ssp. mexicana, an annual wild relative of maize, has many desirable characteristics for maize improvement. To transfer alien genetic germplasm into maize background, F1 hybrids were generated by using Z. mays ssp. mexicana as the female parent and cultivated maize inbred line Ye515 as the male parent. Alien introgression lines, with a large range of genetic diversity, were produced by backcross and successive self-pollinations. A number of alien introgression lines with the predominant traits of cultivated maize were selected. Genomic in situ hybridization (GISH) proved that small chromosome segments of Z. mays ssp. mexicana had been integrated into the maize genome. Some outstanding alien introgression lines were evaluated in performance trials which showed 54.6% hybrids had grain yield greater than that of hybrid check Yedan12 which possessed 50% Ye515 parentage, and 17.1, 9.9% hybrids had grain yield competitive or greater than those of Nongda108 and Zheng958, which were elite commercial hybrids in China, respectively. The results indicated that some of the introgression lines had excellent agronomic traits and combining ability for maize cultivar, and demonstrated that Z. mays ssp. mexicana was a valuable source for maize breeding, and could be used to broaden and enrich the maize germplasm.  相似文献   

4.
Summary The tripartite hypothesis postulates that cultivated maize was derived from a wild podcorn which was once indigenous to the lowlands of South America; that this wild Z. mays is now extinct; that Z. mexicana (teosinte) originated from natural hybridization of Z. mays and a species of Tripsacum after cultivated maize was introduced into Central America; and that most modern races of maize resulted from introgression of primitive maize with teosinte, Tripsacum, or both. This hypothesis has been criticized primarily on the basis that it involves too many theories for the available facts.The basic question that needs to be answered is whether maize was domesticated from a wild Z. mays or from Z. mexicana. The oldest known archaeological remains suggest that a wild Z. mays existed before Z. mexicana came on the scene. This question can only be settled by further archaeological research.Accepting the assumption that a species of Zea which is distinctly different from Z. mexicana gave rise to maize, however, does not necessarily lead to accepting a hybrid origin for teosinte. Judging from present day breeding behaviour, Z. mays and Z. mexicina are conspecific and only distantly related to Tripsacum. This cytogenetic similarity could be explained on the basis of extensive introgression between teosinte and maize. Introgression of germplasm from teosinte into maize is obvious wherever these taxa are sympatric. This pattern of a crop absorbing genes of its wild progenitor across a weedy race, is commonly encountered in all other cereals. Should this not be true in maize, it certainly will be a rare exception.The assumption that teosinte originated as a hybrid between domesticated Z. mays and a species of Tripsacum remains an intriguing possibility. However, after weighing the pros and cons of the available morphological and genetical evidence, the level of confidence with which this hypothesis can be excepted appears rather low.  相似文献   

5.
Adventitious root formation (ARF) at the soil surface is one of the most important adaptations to soil flooding or waterlogging. Quantitative trait loci (QTL) controlling ARF under flooding condition were identified in a 94 F2 individual population by crossing maize (Zea mays L., B64) × teosinte (Z. mays ssp. huehuetenangensis). A base-map was constructed using 66 SSR and 42 AFLP markers, covering 1,378 cM throughout all ten maize chromosomes. The ARF capacity for seedlings was determined by evaluating the degree of root formation at the soil surface following flooding for 2 weeks. ARF showed continuous variation in the F2 population. Interval mapping and composite interval mapping analyses revealed that the QTL for ARF was located on chromosome 8 (bin 8.05). Utilising a selective genotyping strategy with an additional 186 F2 population derived from the same cross combination and 32 AFLP primer combinations, regions on chromosomes 4 (bin 4.07) and 8 (bin 8.03) were found to be associated with ARF. Z. mays ssp. huehuetenangensis contributed all of the QTL detected in this study. Results of the study suggest a potential for transferring waterlogging tolerance to maize from Z. mays ssp. huehuetenangensis.  相似文献   

6.
Origin of maize: The tripartite hypothesis   总被引：2，自引：0，他引：2  
Summary The oldest known archaeological race of maize is a pod-popcorn. The closest living wild relative of cultivated maize is teosinte. Although the morphological differences between maize and teosinte are spectacular in appearance they are conspecific genetically. The tripartite hypotheses proposes that the ancestor of domesticated maize was a now extinct wild pod-popcorn; that teosinte originated from maize-Tripsacum hybridization; and that introgression with either teosinte or Tripsacum gave rise to the tripsacoid syndrome characteristic of many modern races of maize. Maize and teosinte cross readily, and exchange genes wherever they are sympatric. Hybrids between maize and Tripsacum are not always easy to produce, but natural introgression between these taxa is possible. However, artificially induced introgression from Tripsacum into maize failed to produce either teosinte-like offspring or the combination of tripsacoid characteristics assumed to indicate such introgression during the evolution of several South American races of maize. The available archaeological data seem to exclude teosinte as a possible ancestor of domesticated maize. This will make maize the only cereal without a living direct ancestor. Biosystematic studies suggest that teosinte is so closely related to domesticated maize that it could be accepted as the progenitor of maize.Supported in part financially by the Illinois Agricultural Experiment Station, and Grant GB-28495 from the National Science Foundation.  相似文献   

7.
To improve haploid plant production in durum wheat, the haplomethod involving intergeneric crossing with maize followed by embryo rescue was used. The influence of parental genotypes and various experimental factors were studied. Ten cultivars of Triticum turgidum ssp. durum (female parent) were crossed with eight genotypes of Zea mays. After pollination, plant stems were either maintained in situ or cut near the base and kept in a 2,4‐dichlorophenoxyacetic acid (2,4‐D)‐sucrose solution. Ten to 18 days after pollination, embryos were excised from developed ovaries and cultured on one of MS, MS/2, or B5 media. Haploid embryos and plants were obtained (78 green haploid plants regenerated in 0 year). The wheat genotype was significant for ovary development, embryo and plant formation, whereas the maize genotype was significant only for embryo formation. Detailed results of all crosses showed the best crossing partner for each wheat genotype. Cutting the plant stems after pollination gave better results than maintaining them in situ. The optimal stage for embryo rescue was 14 days and B5 and MS/2 media were more efficient than MS for embryo culture.  相似文献   

8.
The study was undertaken to evaluate the relative efficiency of different Gramineae genera for haploid induction in triticale (x Triticosecale) and triticale × wheat (Triticum aestivum) hybrids through the chromosome elimination (wheat × maize, Zea mays) system. Eight intergenotypic triticale and 15 triticale x wheat crosses were subjected to hybridization with nine different Gramineae genera viz., Z. mays, Sorghum bicolor, Pennisetum americanum, Setaria italica, Festuca arundinacea, Imperata cylindrica, Cynodon dactylon, Lolium temulentum and Phalaris minor in two separate experiments. This was followed by in vivo auxin treatment of the crossed spikes and subsequent rescue of the haploid embryos to regenerate green haploid plantlets. All the triticale and triticale x wheat crosses resulted in seed set in variable frequencies when hybridized with maize, I. cylindrica, pearl millet and sorghum. Seed set was also obtained with S. italica, F. arundinacea and P. minor in a few crosses in both groups. In general, all the triticale x wheat crosses, except for one in each case, resulted in embryo formation and green haploid plantlet regeneration when hybridizations were carried out with maize and I. cylindrica. However, the latter outperformed the former in embryo formation (25.48% vs. 20.0%) and regeneration (34.17% vs. 15.10%) frequencies, the differences being significant for regeneration frequencies. In the case of triticale hybrids, no significant differences between maize and I. cylindrica were observed for the three parameters of haploid induction. Embryo formation and regeneration were also observed in some of the triticale as well as triticale × wheat F1 hybrids when hybridized with sorghum and pearl millet.  相似文献   

9.
Summary DNA restriction fragment length polymorphism (RFLP) analysis was performed on 50 wild and old cultivated sugarcane accessions. Ninety-four maize low copy nuclear DNA sequences of known chromosomal position were screened for hybridization to digested sugarcane genomic DNA blots. Seventy-five (80%) gave very strong hybridization signals and usually yielded many bands and detected profuse polymorphism. Twenty-nine probes and 36 probe/enzyme combinations were selected on the basis of the scorability of the banding profiles. A total of 1110 fragments were separately identified among the 50 genotypes. Multivariate analyses of the data allowed the separation of the three basic species, Saccharum spontaneum, S. robustum and S. officinarum, showed that S. spontaneum had structure which could be related to the geographic origin of the clones and supported current hypotheses on the origin of secondary species S. barberi and S. sinense. The use of more probes did not improve the resolution between the various species examined but identified a few key polymorphisms which were not accounted for by current phylogenetic hypotheses and can guide future analyses. RFLPs in sugarcane will be useful essentially for depicting the genomic constitution of modern varieties of interspecific origin.  相似文献   

10.
Current agricultural practice favours winter cover crops, which can not only optimize N management in field crop rotation; but also affect subsequent crops. Three field experiments were carried out in Eastern Slovenia to examine the effects of Italian ryegrass (Lolium multiflorum Lam.), winter rape (Brassica napus ssp.oleifera (Metzg.) Sinsk), subclover (Trifolium subterraneum L.), and crimson clover (Trifolium incarnatum L.) as winter cover crops on the mineral N (Nmin) content of soil and on the yield and N content of subsequent maize (Zea mays L.), fertilized with 120 kg N ha−1. Italian ryegrass and winter rape decreased soil Nmin contents before winter and in spring more than both clovers. In contrast, clovers accumulated significantly higher amounts of N in organic matter and had lower C/N ratios than winter rape and especially Italian ryegrass. In comparison to the control (bare fallow without cover crop), clovers increased the whole above ground maize dry matter yield, maize grain yield and N contents in whole above ground plants and in grain. The yields and N contents of maize following winter rape were on the same level as the control, while yields and N contents of maize following Italian ryegrass were, in two of the experiments, at the same level as the control. The effects of Italian ryegrass on the maize as subsequent crop in the third experiment were markedly negative. Maize in the control treatment exploited N much more efficiently than in treatments with cover crops. Therefore, cover crop N management should be improved, especially with a view to optimizing the timing of net N mineralization in accordance with the N demands of the subsequent crop.  相似文献   

11.
The principal characteristics of maize and its two relatives, teosinte and Tripsacum are described. The manifestations of the introgression of teosinte into maize determined from experimental cultures can be recognized in prehistoric maize from Guatemala, Mexico, and the southwestern United States. This introgression, which began at least 2,000 years ago, is still proceeding in Mexico where maize and teosinte commonly grow in the same fields and hybridize frequently. Evidence is presented of the direct introgression of Tripsacum in several races of maize of South America. The introgression of teosinte and Tripsacum has contributed to the evolution of maize through gene recombination, mutagenic effects, and various types of heterotic phenomena. The role of introgression in the improvement of maize is discussed.Presented at a conference entitled The role of introgressive hybridization in the evolution of crop plants and their wild relatives, held at Versailles on 4th January, 1961 by the Section Wild Species and Primitive Forms of Eucarpia (European Association for Research on Plant Breeding).The research reported in this paper was supported in part by a grant from the Rockefeller Foundation and the publication expenses were borne in part by the Maria Moors Cabot Foundation for Botanical Research. I am indebted to Dr. W. C. Galinat for several drawings and to Mr. S. M. Sehgal for the photographs in figure 14.  相似文献   

12.
Downy mildews cause considerable damage to maize (Zea mays L.) worldwide, particularly in the tropical Asia. We have evaluated a set of 42 tropical/sub-tropical maize inbred lines developed in different countries in Asia (India, Thailand and Philippines), and Mexico, for analysing the genetic variability for resistance to sorghum downy mildew [Peronosclerospora sorghi; SDM] and Rajasthan downy mildew [P. heteropogoni; RDM]. Experiments were carried out in replicated trials under artificial infection in field conditions against SDM and RDM at Mandya in Karnataka, India, and Udaipur in Rajasthan, India, respectively, during 1999 and 2000. The study resulted in identification of five inbred lines offering consistent and strong resistance to both SDM and RDM, while several inbred lines revealed resistance only to RDM. It was also revealed that the SDM-resistant inbreds are invariably resistant to RDM, while the RDM-resistant inbreds might show differential responses to the SDM. The maize inbred lines identified in this study with broad-spectrum resistance to downy mildews could be potentially useful for basic and applied research work on downy mildews in tropical Asia. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

13.
cDNA-SRAP标记在玉米氮素营养诊断上的应用初探   总被引：1，自引：0，他引：1  
为探讨对玉米氮素营养状况进行分子诊断的可行性,以大面积推广的玉米( Zea mays)杂交种郑单958为材料,用cDNA-SRAP标记的方法研究了玉米幼苗在不同氮素水平下基因表达的差异,对其中的一些差异表达片段进行了克隆和测序,并通过Real-time PCR鉴定了克隆的差异表达片段的真实性.结果表明:不同的SRAP标...  相似文献   

14.
Potential polymorphism of an (AT)N microsatellite at the 3’end of waxy genes in bread wheat was examined. Primers were designed from a published cDNA sequence of a wheat waxy gene. Polymerase chain reaction (PCR) amplification of genomic DNA from 135 mainly Australian cultivars revealed eight alleles on chromosome 7A. This polymorphic microsatellite is a potential codominant marker for the Wx-A1 locus in breeding programmes. A distinguishable fragment was also amplified from chromosome 7D. This fragment was absent where a plant was null for the waxy gene on chromosome 7D, being a dominant marker for the Wx-D1 locus. The primers were also useful for amplifying genomic DNA from barley, rye and triticale and can be used to detect potential polymorphism in these species.  相似文献   

15.
Summary The genetic control of endosperm modification in 12 opaque-2 maize (Zea mays L.) inbred lines was investigated by means of a diallel cross experiment conducted across two environments. Kernel vitreousness and kernel hardness were determined by partially dominant genes. Additive gene action was largely responsible for kernel modification. A favourable general combining ability for kernel vitreousness and kernel hardness was positively correlated with an accumulation of dominant kernel modifying genes. South African sources of endosperm modifiers have been found to be similar to those used in other quality protein maize breeding programmes. Certain inbred lines displayed sufficient genetic potential for use in a quality protein maize hybrid breeding programme.  相似文献   

16.
The germplasm of the potato (S. tuberosum ssp. tuberosum) has been modified since the beginning of this century by breeders who introgressed important agronomic traits, for example disease resistance genes, from several wild and cultivated Solanum species of the Americas. In this paper we show that the Rflp analysis of potato-breeding material can be used to detect chromosomal regions descended from more-distantly related Solanum species. The Rflp patterns of individuals of ten Solanum species, ten breeding lines of S. tuberosum ssp. tuberosum and the cultivated variety ‘Bintje’ were analyzed. Rflp data for each of eight single loci of known genomic position were used for the computation of locus-specific phenograms by distance matrix methods. Several of the potato-breeding lines deviated clearly from the clustered species S. tuberosum, S. stenotomum and S. canasense for one or more of the loci considered. These deviations indicated the presence of “exotic” germplasm at a particular locus. The possibility of detecting such germplasm has implications for mapping the agronomic traits for which the wild species were introgressed and which may still be linked to “foreign” chromosome fragments.  相似文献   

17.
Population structure and relationships within and among 185 accessions of wild (Hordeum vulgare ssp. spontaneum) and cultivated barley (H. v. ssp. vulgare) from five countries in the West Asia and North Africa (WANA) region were studied using 36 simple sequence repeat (SSR) markers. The accessions were divided into subspecies/origin (S/O)‐groups and marker results were analysed in relation to genetic diversity and genetic structure. Wild barley from WANA was found to be highly diverse. The landraces from different countries of the Near East showed genetic diversity that was nearly as high as the wild barley from the same country. Further analyses showed that wild barley from Palestine/Israel represented the group with the highest diversity and the most complex structure. However, this group was distantly related to the cultivated barley in WANA, while the wild barley from the rest of the WANA region was closely related to the cultivated WANA barley. The high diversity and the close relationship to the wild barley make the WANA landraces an interesting genetic resource for both conservation and exploitation.  相似文献   

18.
The use of living mulch (LM) systems for maize (Zea mays L.) cultivation may reduce soil erosion and nitrate leaching. However, the yield of maize cultivated in LM is lower than in conventional farming systems. This decrease has often been attributed to belowground competition, but the lack of a suitable method to demonstrate such competition has prevented further investigation. A recently developed method allows for the direct and non-destructive observation of root growth in LM. Maize expressing a green fluorescence protein was grown in monoculture or together with Italian ryegrass (Lolium multiflorum Lam.). Root growth was screened in minirhizotrons from the time of sowing to the anthesis of the maize.Compared with the maize cultivated without Italian ryegrass, the cultivation in the LM significantly (p < 0.05) reduced the shoot dry matter (56%), leaf area (39%) and mean root density (41%) of the maize at anthesis. An analysis of variance showed that the reduction due to the living Italian ryegrass in the root density of the maize was not significantly different from the reduction in leaf area. Similarly, the reduction in the root density and shoot dry matter of the maize were not significantly different. The roots of the LM maize grew in the soil where the LM was removed but also where the LM continued growing. This study constitutes the first direct quantification of root growth and distribution in a LM system.  相似文献   

19.
Increasing CO2 concentration ([CO2]) is thought to induce climate change and thereby increase air temperatures and the risk of drought stress, the latter impairing crop growth. The objective of this study was to investigate the effects of elevated [CO2] and drought stress on root growth of one maize genotype (Zea mays cv. Simao) and two sorghum genotypes (Sorghum bicolor cv. Bulldozer and Sorghum bicolor × Sorghum sudanense cv. Inka) under the cool moderate climate of Central Europe. It was hypothesized that root growth stimulation due to elevated [CO2] compensates for a reduced root growth under drought stress. Therefore, we established an experiment within a f ree‐a ir c arbon dioxide e nrichment system (FACE) in 2010 and 2011. Sorghum and maize genotypes were grown under ambient [CO2] (385 ppm CO2) and elevated [CO2] (600 ppm CO2) and in combination with restricted and sufficient water supply. Elevated [CO2] decreased root length density (RLD) in the upper soil layers for all genotypes, but increased it in deeper layers. Higher [CO2] enhanced specific root length (SRL) of “Simao” and “Bulldozer,” however, did not affect that of “Inka.” “Simao” achieved a higher SRL than the sorghum genotypes, indicating an efficient investment in root dry matter. Although elevated [CO2] affected the root growth, no interaction with the water treatment and, consequently, no compensatory effect of elevated [CO2] could be identified.  相似文献   

20.
The parasitic witchweed, Striga hermonthica (Del.) Benth, is the greatest biological constraint for cereal crop production by resource-poor farmers in sub-Saharan Africa. Maize, Zea mays L., is a widely grown cereal crop in this region (22 × 106 ha). Striga-resistant maize populations were produced and tested as half-sib families in West and Central Africa. Three populations with white (W), yellow (Y), or mixed (Y/W) grain colour were formed by: (1) intercrossing Striga resistant maize inbred lines followed by two generations of random mating; (2) testing far under artificially induced S. hermonthica infestations in Nigeria, Cameroon, and Ivory Coast and selection of resistant families; (3) two generations of random mating; and (4) two years of testing for resistance. Striga-resistant synthetic W, Y and Y/W populations were produced by compositing resistant half-sib families. Outstanding performance in grain yields and host plant resistance was observed. Maize damage ratings and number of harvested ears were highly correlated with grain yield. High variation was observed for Striga emergence counts. The populations have combined resistance to Striga, maize streak virus (MSV), and other major biotic constraints for maize cultivation in Africa, thus providing the opportunity for improved sustainable maize production under stress environments. Breeder's seed of these synthetic varieties are being multiplied for distribution to national programmes.  相似文献   

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