共查询到19条相似文献，搜索用时 265 毫秒
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利用层析技术对桑黄子实体水提醇沉粗多糖进行了分离纯化,获得不同的组份.采用体外刺激小鼠脾淋巴细胞增殖试验对各组份进行了体外免疫活性的检测,结果表明,水提醇沉的各级粗多糖均有不同程度的体外刺激淋巴细胞增殖的作用;经过离子柱层析分离后,P30得到的各组份均是有活性的,P60得到的各组份中,0W和P60S2活性较好,0S1无活性;分别将P60W和P60S1进行了凝胶柱层析得到均一多糖P60W1-1和P60S1-1,免疫活性比较结果表明P60W1-1有活性,0S1-1无活性,说明有活性的均一多糖必须要从有活性的粗多糖中去分离纯化获得. 相似文献
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《果树学报》2020,(8)
【目的】对籽用西瓜果实瓜瓤多糖进行单糖组成及其一级结构进行鉴定,分析其与生物活性的关系,以评价籽用西瓜多糖的利用价值。【方法】通过水提醇沉法提取籽用西瓜瓜瓤粗多糖,使用Sephadex-G75(葡聚糖凝胶柱层析-G75)和DEAE-Sepharose(DEAE-纤维素柱层析)色谱分离纯化粗多糖得到纯化多糖(SⅠ),利用高效液相色谱法(HPLC)测定其相对分子质量和单糖组成,再通过红外光谱和核磁共振进一步鉴定其一级结构,最后采用体外抗氧化试验和体外抑菌试验分析其生物活性。【结果】纯化多糖SⅠ的HPLC分析结果表明该多糖相对分子质量为1 747 Da,由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、木糖、阿拉伯糖7种单糖组成,摩尔比为2.4∶2.2∶6.6∶36.8∶32.1∶7.2∶12.7;红外光谱分析和核磁共振分析显示SⅠ含有糖类化合物、糖醛酸基团和吡喃糖环的特征吸收峰,多糖中葡萄糖和半乳糖为α-D构型,甘露糖为β-D构型,鼠李糖为β-L构型。SⅠ对羟自由基和DPPH自由基均有清除作用,对Fe~(3+)具有一定还原力。SⅠ对酵母菌有较好的体外抑制效果。【结论】籽用西瓜瓜瓤多糖由多种单糖组成,单糖构型多样,具有较好的抗氧化活性和抑菌活性,在食品和医疗保健等行业中具有很大的开发利用价值。 相似文献
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《食药用菌》2015,(4)
利用超声辅助提取法和分级醇沉得到3种醇沉组分粗多糖CSP40、CSP60和CSP80,并分析这3种组分的抗氧化活性。活性较好的粗多糖CSP40经过DEAE-Sepharose Fast Flow离子柱层析和Sephacryl S-400凝胶柱层析纯化得到单一组分CSP40-2a,进行乙酰化处理后用GC-MS测得其单糖组成。结果表明,超声辅助提取所得的3个醇沉组分均具有较好的抗氧化活性,从强到弱依次为CSP40CSP80CSP60。乙酰化分析表明,CSP40-2a主要由3种单糖组成,分别是D-甘露糖、D-葡萄糖和D-半乳糖,其摩尔比为1.98︰1.0︰0.67。 相似文献
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菌草栽培猴头菌子实体多糖的研究 总被引：1，自引：0，他引：1
以菌草栽培和木屑栽培的猴头菌子实体为试材,采用L9(34)正交实验设计方法对2种猴头菌子实体粗多糖进行提取,研究了不同浸提次数、料液比、浸提时间和温度对猴头菌子实体粗多糖得率的影响;用DEAE SepHarose Fast Flow离子柱层析法和SepHadex G-100凝胶柱层析色谱法对提取出的粗多糖进行初步分离和纯化,以期得到几个组分,且每组分为具有单一对称峰的多糖。结果表明:猴头菌子实体粗多糖提取的最佳条件是,浸提2次,料液比1∶25g·mL-1,浸提时间4h,温度90℃。菌草栽培猴头菌子实体多糖得到3个组分,分别记为cHEP1、cHEP2、cHEP3;木屑栽培猴头菌的子实体多糖纯化后只得到2个组分,分别记为sHEP1、sHEP2。对分离出的cHEP2、cHEP3和sHEP2纯度鉴定结果表明,3种组分均显示为单一对称峰。 相似文献
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长裙竹荪多糖Di-S2P的分离纯化和鉴定 总被引：2，自引：2，他引：2
长裙竹荪 (Dictyophoraindusiata)子实体经 ρ(Na2 CO3 ) =2 %溶液提取 ,用蛋白酶法和Sevag法相结合除去蛋白 ,乙醇分级沉淀 ,级分 2经DEAE -SephadexA -2 5柱层析纯化得到长裙竹荪多糖Di-S2P .经测定该多糖为均一组分 ,分子量约为 8 7× 10 5,红外光谱呈现出典型的多糖吸收峰 ,紫外扫描无核酸和蛋白质的特征吸收峰 .纸层析和气相层析分析得知Di -S2P含有D -葡萄糖、D-半乳糖、D -甘露糖和D -木糖 ,其摩尔比为 1 6 2∶1 87∶1 0 0∶0 93。 相似文献
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一种灵芝硒多糖SeGLP-2A的分离纯化及分子组成分析 总被引：2，自引：1，他引：2
从灵芝 (Ganodermalucidum)加硒深层培养的菌丝中 ,分别经水、碱水提取 ,醇析 ,透析 ,脱色 ,脱蛋白 ,DEAE—Cellulose柱层析和SepharoseCL4B柱层析纯化后 ,得SeGLP - 2A。经SephadexG - 10 0、聚丙烯酰胺凝胶电泳和紫外光谱分析鉴定 ,SeGLP - 2A为均一级分。GC与TLC分析表明 :SeGLP - 2A含有 4种单糖Gal、Xyl、Glu、Rha ,其单糖摩尔比为n (Gal) :n (Xyl) :n (Glu) :n (Rha) =2 5 5 9∶16 30∶1 0 0∶0 86。经红外光谱分析 ,确定SeGLP - 2是由α -糖苷键连接的吡喃多糖 相似文献
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从大孔树脂类型、洗脱剂与上样量等方面确定灰树花中抑糖基化反应化合物的初分离条件,并选取抑糖基活性最高的组分进行薄层层析分析和硅胶柱分离纯化,最后利用高效液相色谱法和傅里叶红外光谱分析化合物。结果表明:采用D101大孔树脂,25 g树脂最佳上样量为2 g,20%乙醇洗脱组分C_3的抑糖基化活性最高。用氯仿∶甲醇(v/v 1∶9)为洗脱液对C_3进行硅胶柱层析纯化,共分离出5个组分C_35～C_39,其中C_38和C_39具有比较强的抑糖基化活性,合并C_38和C_39,再次用氯仿∶甲醇=(v/v 2∶8)为洗脱液进行硅胶柱层析纯化,共分离出5个组分G_1～G_5,薄层层析和HPLC分析分别显示G_3为单一斑点和单一峰型,说明G_3是一种单体化合物,根据红外光谱结果初步推断该化合物为酚类物质。 相似文献
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灵芝硒多糖的制备及其分离纯化 总被引：5，自引：0，他引：5
灵芝（Ganoderma lucidum）菌丝体通过生物转化作用富集无机硒，分别经水、碱水浸提，醇析，去蛋白，脱色后，得到灵芝硒多糖粗提物。再经DEAE—Cellulose柱层析分离纯化后，收集得到的各组分在高效液相色谱上进行进一步纯化，从而得到了10个不同的硒多糖组分。以K562细胞进行的抗癌试验结果显示，SeGLP-2B-1，SeGLP-2B-2和SeGLP-2B-3三种纯化硒多糖均有抗癌活性，其中SeGLP-2B-1对K562细胞的抑制率最高，达61．58％。 相似文献
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棘托竹荪子实体水溶性多糖DE2—2的分离纯化和鉴定 总被引：10，自引：0，他引：10
棘托竹荪（Dictyophora echinovolvata Zang,Zheng et Hu)子实体干品经热水抽提，乙醇沉淀，蛋白酶法和Sevag法去蛋白后得粗多糖DE。DE经乙酥分级沉淀得到三个级分DE1、DE2和DE3。DE2经DEAE-Sephadex A-25柱进一步纯化得到棘托竹荪多糖纯品DE2-2。DE2-2经高效液相色谱法测定为均一物质。红外扫描表明，其具有典型的多糖吸收峰。纸层析和气相色谱分析结果表明，DE2-2的单糖组成为：D-葡萄糖、D-甘露糖、D-半乳糖和L-岩藻糖，其摩尔比为8.68:1.00:1.85:0.74，分子量约为84000。经测定，DE2-2具有一定的抑瘤活性，其抑瘤率为38.93%。 相似文献
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香菇菌丝体中两种新多糖（Le—2—1,Le—2—2）的分离,纯化和鉴定 总被引：4，自引：0，他引：4
用发酵培养72h的香菇菌丝体,经热水提取,乙醇沉淀,再经二次DEAE-纤维素柱分离(Cl-型和B_4O_7)和Sephadex G-100纯化,得到两个香菇多糖——Le-2-1,Le-2-2。经HPLC(μ-Bondgel Linear柱)和醋酸纤维薄膜电泳鉴定,两者都为均一组分,HPLC测得Le-2-1的纯度为99.99%,Le-2-2为100%。气相色谱测单糖组成、摩尔比,HPLC测分子量得Le-2-1为:Gle:Ga1:Man:Xy1:Ara:Rha=86.05:2.48:2.06:21.96:1.98:1.58,分子量:5.7×10~3:Le-2.2为:GlC:Gal:Man:Xyl:Ara:Rha=70.60:10.25:8.51:1.80:2.35:3.16,分子量;5.8×10~4。 相似文献
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AIM: To evaluate the effects of acetyl-11-keto-beta-boswellic acid (AKBA, a main active component from frankincense, one of the traditional Chinese herb for healing wounds) on the activities of matrix metalloproteinase(MMP)-1, MMP-2 and MMP-9.METHODS: Pure human interstitial collagenase (MMP-1) or gelatinase A (MMP-2) was activated by p-aminophenylmercuric acetate (APMA), and was incubated with AKBA for 1 h. The activities of the enzymes were observed by quenched fluorescent substrate. The lysates of rat polymorphonuclear neutrophils ［PMNs, rich in gelatinase B (MMP-9)］ was incubated with AKBA for 1 h, and activity of MMP-9 was tested by gelatin zymography. Three cell models: activated human dermal fibroblasts by TNF-α, activated THP-1 cells by PMA and fibroblasts-THP-1 co-culture system were established. AKBA was cultured with these cell models for 24 h. The levels of MMP-1, MMP-2 and MMP-9 in the cell culture supernatants were tested by ELISA and activities of MMP-2 and MMP-9 were tested by gelatin zymography assays.RESULTS: AKBA dose-dependently inhibited the activities of human MMP-1 and MMP-2 at the range of 0.1-0.8 mmol/L, with 50% inhibitory concentration (IC50) of 0.18 mmol /L and 0.27 mmol/L, respectively. In the range of 0.05-0.85 mmol/L, AKBA inhibited the MMP-9 activity (P<0.01). Although AKBA promoted fibroblasts to secrete MMP-2, the production of MMP-9 by THP-1 was inhibited. In the cell co-culture system, the inhibitory effects on MMP-1, MMP-2 and MMP-9 productions were also observed.CONCLUSION: AKBA, as a bioactive component of frankincense, has an inhibitory effect on MMPs production and activities, indicating the possible mechanism for healing chronic wounds by frankincense. 相似文献
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对豇豆99-2,99-3,99-4三个优良株系性状进行比较试验.结果表明,豇豆99-2具有生长势好、早熟、高产、优质、商品性好等特点,丰产性尤为突出;前期产量12 304.5 kg/hm2,占总产量33 949.5 kg/hm2的36.68%,鲜荚比99-3、99-4早2～3 d上市.可作为早熟品种推广栽培. 相似文献
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E. Westphal 《Scientia Horticulturae》1987,30(4):316-318
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B. Jeffcoat 《The Journal of Horticultural Science and Biotechnology》2013,88(1):143-153
Foliar sprays of the synthetic cytokin, 6-benzylamino-9-(tetrahydropyran-2-yl)-9H-purine (PBA), increased branching in carnation, chrysanthemum, poinsettia, petunia and fuchsia, which showed varying levels of tolerance. In no instance did PBA reduce plant height. Applications of PBA to carnation and chrysanthemum stimulated branching in both intact and pinched plants. Treatment a few days prior to pinching produced more uniform development of new laterals. Response to the compound was most favourable under optimum growing conditions. Under relatively poor light intensity the laterals exhibited a reluctance for continued elongation and showed marked chlorosis. Application of PBA to flower buds at an early stage of development increased both the diameter and the fresh weight of carnation flowers or chrysanthemum inflorescences when fully open. Response to the compound decreased with age of the bud at the time of treatment. In carnation the effects on size were accompanied by an increase in petal area together with an increase in the number of primary petals produced. Relatively high concentrations also induced the formation of secondary growing centres from which varying numbers of additional petals were produced. Relatively large numbers of these secondary centres within a flower resulted in an increased incidence of calyx splitting. These centres were not observed in chrysanthemum. The formation of secondary growing centres could also be induced by GA3 and IAA. However, while these growth substances increased the diameter and fresh weight of carnation flowers, unlike cytokinins they had no apparent effect on the number of primary petals formed. Finally PBA was also observed to increase the longevity of cut flowers of carnation. This was particularly apparent at relatively low temperatures. 相似文献
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金秋梨在室温贮藏的情况下,20d(天)开始腐烂,至30 d(天)出现第一个腐烂高峰,以后20 d(天)开始为稳定期,至70 d(天)后又出现第二个腐烂高峰.室温下随着贮藏时间的延长,呼吸速率逐步增加并在19 d(天)时达到峰值,以后开始迅速下降,然后在53 d(天)出现第二个呼吸峰.金秋梨刚采收时,乙烯释放量极低,低于检测限(10 mol/L～8 mol/L),正常采收期采摘的果实贮藏至第7 d(天)才有少量乙烯释放,释放量为11.15μL/kg·h,采后30 d(天),乙烯释放量达到212.33μL/kg·h,此时金秋梨进入完熟阶段,采后61 d(天),乙烯释放量达到最高289.84μL/kg·h,此时果实已经衰老、萎蔫、腐烂严重,丧失了贮藏价值. 相似文献
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分别用不同浓度(50、100、200mg·L-1)的除草剂安全剂类似化合物乙酰苯胺和2-氯苯磺酰胺处理IN2-2::GUS转基因芥菜。结果显示:乙酰苯胺和2-氯苯磺酰胺处理后,IN2-2启动子在芥菜根、叶、花器官的花萼、花瓣、雄蕊及花粉中表达,但不在胚珠中表达。100mg·L-1乙酰苯胺以及50mg·L-12-氯苯磺酰胺适合用于在芥菜中调控IN2-2启动子的表达,乙酰苯胺较2-氯苯磺酰胺诱导表达所需时间更短。高浓度的2-氯苯磺酰胺影响芥菜种子发芽及生长发育,并且明显抑制IN2-2启动子的表达活性。4℃低温胁迫诱导IN2-2启动子在幼苗叶片中表达,IN2-2启动子轻微受150mmol·L-1Na Cl胁迫表达,但不受重金属Gu~(2+)离子的诱导表达。 相似文献