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1.
This study was conducted in South Wollo Zone of Amhara Regional State, Ethiopia, to assess the milk yield and reproductive performance of indigenous and crossbred cattle under smallholder management conditions. Questionnaire survey was used to collect retrospective data on the performance of dairy cattle in 186 households. Thirty two postpartum cows (16 indigenous and 16 crossbred) were selected purposively and monitored for five months and milk samples were collected twice per week from each cow to determine postpartum ovarian activity using ELISA techniques. Daily milk yields at all stages of lactation were significantly (P < 0.01) higher in crossbred than indigenous cows. Crossbred cows had significantly (P < 0.01) longer lactation length and shorter age at first calving, while calving interval was significantly shorter in cows that gave previous calves during the wet season than the dry season. Only six cows (18.8%) showed heat signs on an average of 136 days postpartum of which one was a crossbred and five were indigenous. While, 11 cows (34.3%) resumed ovarian activity until 150th day postpartum among which three were crossbred and eight were indigenous.  相似文献   

2.
During recent decades, milk production per cow has increased drastically due to improved management, nutrition, and genetic selection; however, the reproductive performance of high-producing dairy cows has been declining. One of the factors responsible for this low reproductive performance is negative energy balance (NEB). NEB affects the onset of first ovulation in early postpartum cows. It is generally accepted that early first ovulation positively relates to the resumption of normal ovarian function, first service, and conception rate in dairy cows. Hence, delayed first ovulation has a negative impact on subsequent fertility. The metabolic condition of cows in NEB shifts to catabolic metabolism, which in turn causes increased plasma growth hormone and non-esterified fatty acid concentrations and decreased plasma insulin-like growth factor-1, insulin, and glucose concentrations. On the other hand, plasma β-carotene concentrations decrease throughout the dry period and reach their nadir in about the first week postpartum, and this change reflects energy balance during the peripartum period. β-Carotene plays a role independently of vitamin A in the reproductive performance of dairy cows, and the positive relationship between supplemental β-carotene and reproductive function has been demonstrated in many studies during the past decades. However, β-carotene content in corn silage, which is a popular main feed in high-producing dairy cows, is very low. This review describes nutritional factors related to ovulation during the first follicular wave postpartum in dairy cows.  相似文献   

3.
Practical aspects of the fertility of dairy cattle   总被引：1，自引：0，他引：1  
To establish sustainability in the dairy industry, it is important that cows become pregnant at a biologically optimal time and at an economically profitable interval after calving. In this review, the results obtained from Holstein cattle in an experimental herd for dairy research are summarized. First, the effect of age at first calving of heifers on productive and reproductive performance was examined. A reduction in calving age from 25.1 to 21.5 months with the same growth rate during the first 12 months after birth had no negative effects on the heifers' performance. Second, the postpartum follicular dynamics of lactating cows were traced in relation to their fertility, and the emergence and fate of cystic ovarian follicles were examined. The premature initiation of ovarian activity does not always improve the fertility of cows as indicated by the number of days open. Third, the occurrences of anestrous ovulation during the early postpartum period were analyzed with reference to the frequency of reversion to anestrus. The premature onset of estrous activity also did not improve fertility, and relapse back into anestrus after the onset of the estrous cycle often occurred during the breeding period. Fourth, some indices for the occurrence of postpartum reproductive events were evaluated as an indicator of the reproductive performance of lactating cows. The milk yield and percentage of body weight loss could be indicators for reproductive events. Finally, the potency of a pedometry system for the detection of typical and atypical estrous behaviors of heifers and lactating cows was evaluated in terms of efficiency and accuracy. The location of the pedometers and housing conditions for the animals affected the estrus detection of the system. These results represent the reproductive potential of modern high-yielding dairy cattle and provide a baseline to evaluate their reproduction.  相似文献   

4.
Studies were conducted in Korean native cattle to determine the age and body weight at puberty, and to determine the postpartum anestrus intervals based on plasma progesterone profiles, rectal palpation, ultrasonography and estrus observation. Korean native cattle reached puberty at 344 days of age and at 188 kg body weight as determined by plasma progesterone profiles, but estrous behavior was not detected until 3 months later, at a body weight of 215 kg. Prolonged postpartum anestrus occurred in 40% of smallholders cows, where animals were tethered and fed hay and rice straw, whereas its incidence was only 6.8% in a well managed herd that was grazed and housed. Plasma progesterone profiles were used to monitor ovarian activity postpartum in standard fed cows (n=44) and restricted fed cows (n=20). In standard fed cows 81.8% showed luteal activity by 50 days postpartum, compared to 55% in restricted fed cows. Approximately 40% of cows in both groups had shown observable estrous behavior by this time. This study indicates that one or more ovulations had occurred before pubertal heifers and postpartum cows showed overt estrous behavior. It was concluded that improved management and feeding practices should result in improved reproductive performance.  相似文献   

5.
Nutrition has long been known to have a profound influence on reproductive performance of female cattle, but the underlying mechanism remains poorly understood. Whilst early investigations focused on the modulation of nutrition on hypothalamic-pituitary axis, more recent studies have tested the hypothesis that metabolic hormones as nutritional signals exert a direct effect at the ovarian level. In cattle, treatment with recombinant bovine somatotrophin (rGH) significantly increases the population of small ovarian follicles. This is associated with increases in circulating concentrations of insulin and insulin-like growth factor-I (IGF-I). Subsequent studies, both in vitro and in vivo, have highlighted the importance of IGF-I and/or insulin acting in synergy with FSH and LH. More recently, we demonstrated that feeding heifers with 200% maintenance requirements for a short period significantly increases circulating insulin concentrations and population of small ovarian follicles. Based on these findings, our recent work has aimed at addressing some practical problems in cattle production. Firstly, we showed that both rGH pretreatment and increased dietary intake significantly enhance the response to standard superovulatory regimes. Secondly, we have demonstrated that feeding a diet to increase circulating insulin concentrations during the early lactation can advance the first ovulation postpartum and increase conception rate to the first service in dairy cows. In summary, nutrition influences ovarian follicle development in cattle possibly through changes in metabolic hormones. These interactions can be manipulated to improve reproductive performance.  相似文献   

6.
A total of 51 pluriparous post-partum Bunaji (Zebu) cows belonging to agropastoralists were involved in a 2 x 2 factorial experiment for a period of 180 days. The cows were assigned randomly at calving to four treatments: (1) grazing only (no supplementation) and exposure to bull (NSBE); (2) grazing only (no supplementation) and no exposure to bull (NSNE); (3) grazing plus feed supplementation (each cow received 600g of 20.8% crude protein of whole cottonseed supplement per day) and exposure to bull (FSBE); (4) grazing plus feed supplementation and no exposure to bull (FSNE). Cows with an increase in milk progesterone (P(4)) concentration of > or =1 ng/mL from the weekly milk samples were used to analyse the number of days from calving to the time of resumption of ovarian activity. The mean interval from parturition to cyclic ovarian activity for FSBE cows was 95 days, compared to 119 days for the FSNE cows. Intervals to onset of post-partum ovarian activity were 24, 33 and 39 days which were significantly earlier in the FSBE cows, than the FSNE, NSBE and NSNE cows, respectively. Intervals to cyclic activity were 9 and 15 days earlier in FSNE cows than in NSBE and NSNE cows. By 150 days post-partum, 100% and 92% of the cows in the supplemented groups (FSBE and FSNE), had resumed cyclic ovarian activity compared with 75% and 69% for the unsupplemented cows (NSBE and NSNE). It is concluded that nutritional supplementation and exposure to bulls synergistically shortened the length of post-partum anoestrus in zebu cattle. The economic benefits of using exposure to bulls and cottonseed supplementation in this study to enhance early resumption of post-partum ovarian activity of cattle may serve as a management tool in tropical areas where livestock production has some constraints.  相似文献   

7.
The aim of our study was to investigate the effects of suckling on reproductive performance and metabolic status of obese (mean body condition score of more than 4.0 on a scale of 1-5) maternal Japanese Black cows during early postpartum period. We used 7 postpartum Japanese Black cattle. Four cows were suckled ad libitum (suckled) until completion of their first artificial insemination (AI), while 3 cows were not suckled at all because they were separated from their calves immediately after parturition (non-suckled). Body weight and plasma concentrations of metabolites and hormones were measured from wk 1 to 9 postpartum. Ovarian activity was detected using plasma progesterone concentration, and all cows received their first AI after application of the Ovsynch protocol at approximately 4 months postpartum. Although body weights of non-suckled cows increased during experimental period (P<0.05), those of suckled cows remained unchanged. Plasma concentrations of glucose of non-suckled cows were higher at wk 2 postpartum (P<0.05) and their levels of non-esterified fatty acid tended to be lower at wk 1 and 2 postpartum compared with suckled cows (P<0.1); however, these differences between groups were not observed with progression of postpartum period. In addition, plasma insulin concentrations of non-suckled cows were higher than those of suckled cows during experimental period (P<0.05). During sampling period (wk 0 to 9 postpartum), onset of normal ovarian cycle was observed in all non-suckled and 2 of 4 suckled cows, and it was delayed in other 2 suckled cows compared with non-suckled cows; however, 3 suckled cows conceived at the first AI after application of the Ovsynch protocol; none of non-suckled cows conceived at this time. Overall, we suggest that suckling seems to reduce increase of body weight after parturition, although it does not improve obesity, and influences conception despite delay in resumption of normal ovarian cyclicity in obese Japanese Black cows.  相似文献   

8.
现代规模化、集约化奶牛养殖生产模式下,不断供给高营养水平日粮是维持后备牛快速生长发育和泌乳母牛高泌乳性能的物质基础。然而,在遗传品质到达"瓶颈期"后,尽管随着日粮营养浓度的不断提高和干物质采食量（DMI）的增加,奶牛单产水平可以继续维持在一定的高水平状态,但是高产奶牛的营养代谢性疾病的发病率也呈现快速增长趋势,特别是围产期能量负平衡（negative energy balance,NEB）引起的酮病,高精日粮引发的酸中毒、高蛋白质日粮引起的血液中尿素氮升高等营养代谢性疾病,负调控奶牛繁殖性能,造成奶牛产后发情不明显,配种率和受胎率下降等,直接影响奶牛生产群的更新速度、优质牛群泌乳性能正常发挥以及奶牛养殖的经济效益。作者详细介绍了近年来国内外不同营养代谢性疾病对奶牛繁殖性能影响的相关研究资料,重点分析了酮病、低血钙、瘤胃酸中毒等围产期高发的营养代谢性疾病影响奶牛繁殖性能的分子机制,并对营养代谢性疾病与高产奶牛繁殖性能今后研究的方向提出了展望和思考,以期为提高中国规模化牧场高产奶牛群繁殖效率和母牛围产期营养管理水平提供一定的借鉴和理论依据。  相似文献   

9.
Ten reared cows of a Japanese Black cattle herd in Kagoshima prefecture, Japan, exhibited extremely low blood urea nitrogen (BUN) concentration (2.6 ± 0.6 mg/dL). Examination of dietary feed nutrition and relevant pastureland soil content suggested a correlation with crude protein (CP) deficiency or unbalanced nutritional dietary feeds. Thirteen months after the introduction of a dietary remedial measure (bean cake supplementation), BUN, total cholesterol and albumin concentration from five of the original 10 cows increased significantly compared with their values of before the dietary remedy. The postpartum day open period was significantly lower after the dietary remedial measure than that before it. The abnormally low BUN levels of the cattle herd may be due to inadequate dietary nutritional content, primarily from the imbalance of total digestible nutrient and CP of the feed and far lower han average CP value. In conclusion, routine examination of serum biochemical parameters in Japanese Black breeding cattle may be a useful strategy for determining subclinical metabolic failure of cattle herds, and consequently, its effect on reproductive performance of the herd.  相似文献   

10.
Under the modern large-scale and intensive dairy farming production mode,the continuous supply of feed with high nutrition level is the material basis for maintaining the rapid growth and development of reserve cows and the high lactation performance of lactating cows.However,after the genetic quality reaches the "bottleneck period",although the milk yield of dairy cow can continue to maintain a certain high level with the continuous improvement of the nutrient concentration of feed and the increase of dry matter intake (DMI),the incidence of nutritional metabolic diseases of high-yield dairy cows also shows a rapid growth trend,especially ketosis caused by the negative energy balance in perinatal period,acidosis caused by high-precision diet and the increase of blood urea nitrogen caused by high-protein diet,negatively regulate the reproductive performance of dairy cows,resulting in inconspicuous postpartum estrus,decreased mating rate and conception rate of dairy cows,which directly affect the update speed of dairy cows production groups,the normal performance of high-quality cattle and the economic benefits of dairy farming.In this paper,the author introduced in detail the relevant research on the current situation and its mechanism influence of different nutritional metabolic diseases on the reproductive performance of dairy cows in recent years,and focused on analyzing the molecular mechanism of nutritional and metabolic diseases with high perinatal incidence such as ketosis,low blood calcium and moderate gastric acid,and put forward the prospect and thinking on the future research direction of nutritional and metabolic diseases and reproductive performance of high-yielding dairy cows,in order to provide some reference and theoretical basis for improving the reproductive efficiency of high-yielding dairy cows in large-scale pastures in China and the perinatal nutrition management level of dairy cows.  相似文献   

11.
Mammals such as cattle, swine, sheep and humans are born with a highly variable number of ovarian follicles and oocytes in the ovaries that dwindle during ageing and are never replenished. This variation in the ovarian reserve is reflected in the numbers of antral follicles in the ovaries at all ages after birth. As numbers of follicles in ovaries are determined during gestation, the role of maternal nutrition and health during gestation (at time of ovarian development in their foetuses) has been investigated as factors that may impact oogonia proliferation and thus follicle numbers post-natally. These studies have found that both nutrition and health impact numbers of follicles in their offspring. The idea that numbers of follicles and oocytes in ovaries impact fertility is a long-held belief in reproductive biology. This has recently been tested in cattle, and it has been shown that cows with a relatively high number of antral follicles in ovaries have higher pregnancy rates, shorter calving to conception intervals and fewer artificial inseminations during the breeding season compared with cows with a lower number of follicles, and similarly, heifers with many follicles had higher pregnancy rates than those with fewer follicles. Studies summarized in this review highlight the importance of the maternal environment during gestation in determining the size of the ovarian reserve in their offspring and also the contribution of the ovarian reserve to subsequent fertility in cattle.  相似文献   

12.
The aim of this study was to investigate the relationship between the first ovulation within 3 weeks postpartum and subsequent ovarian cycles and fertility in high producing dairy cattle in Hokkaido, Japan. In Experiment 1, 110 cows (44 primiparous and 66 multiparous) were used to determine the effects of the first ovulation within 3 weeks postpartum on subsequent ovarian cycles. Milk samples were collected twice weekly from 7 to 100 days postpartum. The first ovulation was identified by an increase in milk progesterone (P4) to more than 1 ng/ml within 3 weeks postpartum. The numbers of cows showing ovulation and anovulation within 3 weeks postpartum were 31 (70.5%) and 13 (29.5%) in the primiparous cows and 35 (53.0%) and 31 (47.0%) in the multiparous cows, respectively. The patterns of ovarian resumption after calving were classified into two types (normal ovarian cycles and abnormal ovarian cycles) on the basis of milk P4 concentrations. Initiation of normal ovarian function in cows ovulated within 3 weeks postpartum occurred earlier than in anovulated cows regardless of the number of calvings (primiparous, 27.8 days vs. 44.4 days; multiparous, 30.6 days vs. 55.7 days; P<0.01). Out of the multiparous cows that ovulated within 3 weeks postpartum, initiation of normal ovarian function followed by a normal luteal phase was earlier than when it was followed by an abnormal luteal phase (25.5 days vs. 40.4 days; P<0.05). Milk P4 concentrations after the first ovulation were lower than those after the second ovulation in both the primiparous and multiparous cows (P<0.05). In Experiment 2, 22 multiparous cows were used to determine the effects of the first ovulation within 3 weeks postpartum on subsequent fertility. Blood samples were collected once a week from 0 to 3 weeks postpartum. The interval from parturition to first service in ovulated cows was shorter than in anovulated cows (68.4 days vs. 94.8 days; P<0.05). The conception rate by 100 days after calving tended to be higher in ovulated cows than in anovulated cows (50.0% vs. 16.7%, P=0.09). In conclusion, our data strongly suggests that ovulation within 3 weeks postpartum is a crucial phenomenon for subsequent resumption of ovarian function and conception, and thus it can be used as an index of subsequent reproductive performance.  相似文献   

13.
The objectives of this study were to assess changes in nutritional status/body condition score, percentage pregnancy and calving rate in Zebu cows and to establish the relationship between body condition score loss and postpartum interval to resumption of ovarian activity. A total of 198 cows and postpubertal heifers of the Tanzanian Shorthorn Zebu kept under a traditional management system were randomly selected from 200 smallholder herds. Factors investigated during bi-weekly visits include body condition score (BCS), heart-girth circumference, milk yield and reproductive status of the animals, pregnancy/non-pregnancy and cyclicity/non-cyclicity. Local weather conditions and dates of occurrence of other reproductive events such as calving were also recorded. Calvings occurred all year round but with a strong seasonal distribution characterized by annual peaks observed between April and July. The annual calving pattern was closely related to rainfall, with peak precipitation occurring a few months before peak calving. The overall percentage pregnancy varied from 30% to 50% throughout the year. Postpartum cows exhibited minimum mean BCS and heart-girth circumference 12–14 weeks after calving, and cows with BCS loss >1 point exhibited the longest time interval from calving to onset of ovarian activity.  相似文献   

14.
This report reviews the most recent developments in prostaglandin‐based oestrous synchronization programmes for postpartum dairy cows and addresses the efficiency of controlled breeding protocols based on such developments for cows with abnormal ovarian conditions. A double prostaglandin protocol applied 11–14 days apart seems to be capable of bringing most cows to oestrus. Because of varying oestrus onset times, improved conception rates are obtained following artificial insemination (AI) at detected oestrus rather than fixed‐time AI in prostaglandin‐treated cows. The administration of oestradiol or human chorionic gonadotrophin, or both these hormones, after prostaglandin treatment, improves the synchrony of oestrus yet does not enhance the conception rate. Progesterone‐based treatments for oestrous synchronization are considered the most appropriate for non‐cyclic or anoestrous postpartum dairy cows; prostaglandin alone being ineffective because of the absence of a mature corpus luteum in these cows. Improved oestrus synchrony and fertility rate have been reported using short‐term progesterone treatment regimes (7–9 days) with or without oestradiol benzoate combined with the use of a luteolytic agent given 1 day before, or at the time of, progesterone withdrawal. The ovulation synchronization (Ovsynch) protocol, based on the use of gonadotrophin releasing hormone and prostaglandin, was developed to coordinate follicular recruitment, CL regression and the time of ovulation. This protocol allows fixed time insemination and has proved effective in improving reproductive management in postpartum dairy cows. However, timed AI following Ovsynch seems to have no beneficial effects in heifers, because of an inconsistent follicle wave pattern, and in anoestrous cows, given their lack of prostaglandin responsive CL. To date, there are several prostaglandin based, fixed‐time insemination oestrous synchronization protocols for use in early postpartum dairy cows with ovarian disorders such as ovarian cysts and acyclicity.  相似文献   

15.
The objectives of this study were to determine the risk factors for development of postpartum ovarian cysts by evaluating several reproductive factors in individual cows, and to determine the economic impact of ovarian cysts on subsequent reproductive performance in dairy herds in Korea. The data, including cow parity, abnormal puerperium, endometritis, body condition score (BCS), and breeding status were collected from 634 cows in 9 dairy herds. We used logistic regression to evaluate the effects of these factors on ovarian cysts. A stepwise procedure, used to obtain the appropriate model with alpha=0.05, revealed that cow parity was the most important risk factor for ovarian cyst development within 8 weeks postpartum, while development of endometritis and BCS loss>or=1 from the dry period to 8 weeks postpartum were the most important risk factors for ovarian cyst development beyond 8 weeks postpartum. The occurrence of ovarian cysts beyond 8 weeks postpartum prolonged (P<0.01) the mean intervals from calving to first service (27 days) and conception (77 days), and increased (P<0.05) the culling rate (7.8%), while ovarian cyst development within 8 weeks postpartum did not affect (P>0.05) the mean intervals from calving to first service and conception or the culling rate. The economic loss resulting from the occurrence of ovarian cysts was estimated at approximately 823,996 won ($687) due to effects on the cost of nutrition, average growth of calves, labor and medical costs, and culling. These results suggest that cow parity is correlated with the development of ovarian cysts within 8 weeks postpartum, and endometritis and BCS loss>or=1 from the dry period to 8 weeks postpartum are correlated with the development of ovarian cysts after 8 weeks postpartum, which decreases reproductive performance and results in economic loss in dairy herds in Korea.  相似文献   

16.
The relationship among nutritional status, systemic insulin-like growth factor-I (IGF-I) and ovarian function early postpartum were investigated. A total of 27 Holstein-Friesian cows, 10 that cycled normally within 20 days postpartum, 5 diagnosed with follicular cysts, 8 with persistent corpus luteum (CL) after the first ovulation postpartum and 4 with inactive ovaries were used for the study. Blood samples were collected 1-3 times per week, for 60 days pre- and postpartum, for IGF-I, progesterone, estradiol, free fatty acids (FFA), blood urea nitrogen (BUN), and aspartate aminotransferase (AST) determination. Inactive ovary and cystic cows had a higher body condition score before calving and lost more condition than normal or persistent CL cows. Immediately postpartum, IGF-I levels were higher and rose sharply in cows that cycled normally than in cystic, inactive ovary or persistent CL cows. At calving and early postpartum, FFA was higher in inactive ovary and cystic than in normal and persistent CL cows. There was a significant strong positive relationship between IGF-I and BUN, and strong negative relationships between IGF-I and FFA and AST in all groups. There was a positive relationship between serum IGF-I and estradiol in normal cystic and inactive ovary cows. This study found that overconditioned cows during the dry period or at calving, lost more body condition postpartum. These cows also had a deeper and longer period of negative energy balance (NEB), poor liver function and low circulating IGF-I concentrations early postpartum. Such cows were likely to have poor reproductive function as seen in development of cystic ovaries, persistent CL and inactive ovary. Changes in serum IGF-I early postpartum may help predict both nutritional and reproductive status in dairy cattle.  相似文献   

17.
Social cues, often in the form of priming pheromones, can retard or enhance the rate of sexual development in a variety of mammals. The complex interactions between the social environment and reproduction have been explored most thoroughly in the house mouse. A urinary pheromone produced by females in a group inhibits sexual development, and a urinary pheromone from adult males accelerates onset of puberty in juvenile females. These priming pheromones apparently are detected by the vomeronasal organ and induce the changes in ovarian function via changes in the hypothalamic-pituitary system. Accelerated onset of puberty is not accompanied by deficits in reproductive performance. Puberty acceleration can have important management implications for domestic farm animals. It already is proving useful for manipulation in rearing swine and in synchronizing seasonal reproductive recrudescence in sheep. In cattle, the results are less clear that signals from the bull can hasten onset of puberty in heifers. The effect may be operable only under certain nutritional or other interacting conditions. Presumably, the postpartum anestrus period in the cow can be shortened by stimulation from the bull, although pheromones have not been implicated in this effect.  相似文献   

18.
高丽华 《饲料研究》2021,(2):132-134
在规模化牛场的生产过程中,品种、营养、牛群结构、饲养管理、疾病防治等因素对奶牛生产及经济效益有很大影响。而选择高产品种、调整营养水平、建立合理的牛群结构、采取适宜的饲养管理和疾病防治措施,不仅能够提高奶牛的生产性能、繁殖性能,还能降低饲养成本、提高经济效益。文章对影响奶牛生产性能及经济效益的因素进行论述,为促进我国规模化奶牛业的持续健康发展提供参考。  相似文献   

19.
The current review aims to establish insulin-like growth factor-1 (IGF-I) as the factor that signals nutritional status to the reproductive axis, and show that assessment of IGF-I in blood early postpartum during the negative energy balance (NEB) period could be used to predict both nutritional and reproductive status in dairy cattle. The review also explores the effect of nutritional status on circulating IGF-I concentrations and the endocrine role of IGF-I on the reproductive axis. IGF-I plays an important role in gonadotropin-induced folliculogenesis, ovarian steroidogenesis and corpus luteum (CL) function. It also modulates pituitary and hypothalamus function. IGF-I clearly has an endocrine role on the reproductive axis. Severe under nutrition significantly reduces plasma IGF-I concentrations. During the critical period of NEB in high yielding dairy cattle early postpartum, IGF-I concentrations are low in blood and its levels are positively correlated to energy status and reproductive function during this period. Changes in circulating IGF-I immediately postpartum may help predict both nutritional and reproductive status in dairy cattle. IGF-I is therefore one of the long sought factors that signal nutritional status to the reproductive axis.  相似文献   

20.
Survey data on dairy cattle production were gathered in two sites [Site I (three-year survey) and Site II (two-year survey)] in Southern Luzon, Philippines. Crossbred (Holstein-Friesian x Sahiwal) dairy cows (n = 122) managed by smallholder farmers belonging to five primary cooperatives under the federation of dairy farmers, were monitored monthly for milk production, feed intake and availability, and reproduction and health status. The purpose of the survey was to identify constraints to productivity. The reproductive status of the cows was monitored by measuring milk and plasma progesterone concentrations by radioimmunoassay and rectal palpation of the ovaries. Plasma concentrations of selected metabolites [beta-hydroxybutyrate (BHB), inorganic phosphorus, albumin, globulin, urea] were also measured at one month before calving and at one month and 2-3 months postpartum, to determine if these could serve as biochemical indicators of nutritional stress. A long calving interval (CI = > 400 days) was identified as the major constraint to productivity of dairy cattle on smallholder farms. The three main problems related to this reproductive constraint were: (1) poor breeding management, in particular lack of accurate estrus detection; (2) repeat breeding, i.e. three or more services were required before conception; and (3) poor ovarian function, shown by some cows with lose progesterone levels. An important cause of these problems was undernutrition, particularly at critical periods of the cow's reproductive life, reflected in the slow recovery from loss in body weight and condition score during the early postpartum period and the increased plasma BHB values at peripartum period in some cows, indicative of negative energy balance, and the flat lactation profile. These findings are useful and relevant as a database in the development of an appropriate management scheme aimed toward improving dairy cattle production and productivity at smallholder level. It highlights the importance of estrus detection, good breeding management and the use of a practical strategic nutritional supplementation, particularly during stressful periods in the cows's reproductive life.  相似文献   

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