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1.
Intensive greenhouse vegetable‐production systems commonly utilize excessive fertilizer inputs that are inconsistent with sustainable production and may affect soil quality. Soil samples were collected from 15 commercial greenhouses used for tomato production and from neighboring fields used for wheat cropping to determine the effects of intensive vegetable cultivation on soil microbial biomass and community structure. Soil total nitrogen (N) and organic‐matter contents were greater in the intensive greenhouse tomato soils than the open‐field wheat soils. Soil microbial carbon (C) contents were greater in the greenhouse soils, and soil microbial biomass N showed a similar trend but with high variation. The two cropping systems were not significantly different. Soil microbial biomass C was significantly correlated with both soil total N and soil organic matter, but the relationships among soil microbial biomass N, soil total N, and organic‐matter content were not significant. The Biolog substrate utilization potential of the soil microbial communities showed that greenhouse soils were significantly higher (by 14%) than wheat soils. Principal component (PC) analysis of soil microbial communities showed that the wheat sites were significantly correlated with PC1, whereas the greenhouse soils were variable. The results indicate that changes in soil microbiological properties may be useful indicators for the evaluation of soil degradation in intensive agricultural systems.  相似文献   

2.
Ecological soil functions are protected in Germany. Thus, for the sustainable use of urban soil resources data on the function of soils to serve as a habitat are required. Soil microbial biomass and activities were studied in two surface horizons in two consecutive years at nine sites in Stuttgart, Germany, differing in land use. Microbial biomass (chloroform‐fumigation extraction, substrate‐induced respiration) and microbial activities (potential N mineralization, potential ammonium oxidation, and enzyme activities of dehydrogenase, urease, arylsulfatase, and phosphatase) were determined in 2001 and 2002. DIN/ISO standard methods were applied as far as they were available. Furthermore, soil chemical properties were determined in the 2001‐samples. Large differences in chemical and microbiological properties among surface horizons were found. Concentrations of microbial biomass and microbial activities were, however, often comparable to agricultural or forest surface soils. The lowest microbial biomass and activities were observed at a highly disturbed railway area where vegetation was missing and total organic C (TOC) had been altered by anthropogenic organic particles. In contrast, microorganisms were promoted at vegetated sites and where organic impurities were negligible. As TOC was altered by obscure organic matter, total N (TN) and not TOC closely correlated with soil microbiological properties. Biomass and activity generally decreased with depth, but mixing of organic matter resulted in more uniform depth distribution of microbial properties in one garden soil. In 2002, microbial biomass and activity were often lower compared to 2001, but interpretation of this difference hampered as the number of samples taken was probably not sufficient to address the spatial variability in soil properties. Additional studies are needed to develop simple and cost‐effective procedures for the evaluation of ecological quality of urban soils by combined efforts of city planners and soil scientists.  相似文献   

3.
 The effect of long-term waste water irrigation (up to 80 years) on soil organic matter, soil microbial biomass and its activities was studied in two agricultural soils (Vertisols and Leptosols) irrigated for 25, 65 and 80 years respectively at Irrigation District 03 in the Valley of Mezquital near Mexico City. In the Vertisols, where larger amounts of water have been applied than in the Leptosols, total organic C (TOC) contents increased 2.5-fold after 80 years of irrigation. In the Leptosols, however, the degradability of the organic matter tended to increase with irrigation time. It appears that soil organic matter accumulation was not due to pollutants nor did microbial biomass:TOC ratios and qCO2 values indicate a pollutant effect. Increases in soil microbial biomass C and activities were presumably due to the larger application of organic matter. However, changes in soil microbial communities occurred, as denitrification capacities increased greatly and adenylate energy charge (AEC) ratios were reduced after long-term irrigation. These changes were supposed to be due to the addition of surfactants, especially alkylbenzene sulfonates (effect on denitrification capacity) and the addition of sodium and salts (effect on AEC) through waste water irrigation. Heavy metals contained in the sewage do not appear to be affecting soil processes yet, due to their low availability. Detrimental effects on soil microbial communities can be expected, however, from further increases in pollutant concentrations due to prolonged application of untreated waste water or an increase in mobility due to higher mineralization rates. Received: 28 April 1999  相似文献   

4.
 In arable soils in Schleswig-Holstein (Northwest Germany) nearly 30% of the total organic C (TOC) stored in former times in the soil has been mineralized in the last 20 years. Microbial biomass, enzyme activities and the soil organic matter (SOM) composition were investigated in order to elucidate if a low TOC level affects microbial parameters, SOM quality and crop yield. Microbial biomass C (Cmic) and enzyme activities decreased in soils with a low TOC level compared to soils with a typical TOC level. The decrease in the Cmic/TOC ratio suggested low-level, steady-state microbial activity. The SOM quality changed with respect to an enrichment of initial litter compounds in the top soil layers with a low TOC level. Recent management of the soils had not maintained a desirable level of humic compounds. However, we found no significant decrease in crop yield. We suggest that microbial biomass and dehydrogenase and alkaline phosphatase activities are not necessarily indicators of soil fertility in soils with a high fertilization level without forage production and manure application. Received: 12 December 1997  相似文献   

5.
 Soil organic matter level, mineralizable C and N, microbial biomass C and dehydrogenase, urease and alkaline phosphatase activities were studied in soils from a field experiment under a pearl millet-wheat cropping sequence receiving inorganic fertilizers and a combination of inorganic fertilizers and organic amendments for the last 11 years. The amounts of soil organic matter and mineralizable C and N increased with the application of inorganic fertilizers. However, there were greater increases of these parameters when farmyard manure, wheat straw or Sesbania bispinosa green manure was applied along with inorganic fertilizers. Microbial biomass C increased from 147 mg kg–1 soil in unfertilized soil to 423 mg kg–1 soil in soil amended with wheat straw and inorganic fertilizers. The urease and alkaline phosphatase activities of soils increased significantly with a combination of inorganic fertilizers and organic amendments. The results indicate that soil organic matter level and soil microbial activities, vital for the nutrient turnover and long-term productivity of the soil, are enhanced by use of organic amendments along with inorganic fertilizers. Received: 6 May 1998  相似文献   

6.
Declining rates of soil respiration are reliably observed during long-term laboratory incubations. However, the cause of this decline is uncertain. We explored different controls on soil respiration to elucidate the drivers of respiration rate declines during long-term soil incubations. Following a long-term (707 day) incubation (30 °C) of soils from two sites (a cultivated and a forested plot at Kellogg Biological Station, Hickory Corners, MI, USA), soils were significantly depleted of both soil carbon and microbial biomass. To test the ability of these carbon- and biomass-depleted (“incubation-depleted”) soils to respire labile organic matter, we exposed soils to a second, 42 day incubation (30 °C) with and without an addition of plant residues. We controlled for soil carbon and microbial biomass depletion by incubating field fresh (“fresh”) soils with and without an amendment of wheat and corn residues. Although respiration was consistently higher in the fresh versus incubation-depleted soil (2 and 1.2 times higher in the fresh cultivated and fresh forested soil, respectively), the ability to respire substrate did not differ between the fresh and incubation-depleted soils. Further, at the completion of the 42 day incubation, levels of microbial biomass in the incubation-depleted soils remained unchanged, while levels of microbial biomass in the field-fresh soil declined to levels similar to that of the incubation-depleted soils. Extra-cellular enzyme pools in the incubation-depleted soils were sometimes slightly reduced and did not respond to addition of labile substrate and did not limit soil respiration. Our results support the idea that available soil organic matter, rather than a lack microbial biomass and extracellular enzymes, limits soil respiration over the course of long-term incubations. That decomposition of both wheat and corn straw residues did not change after major changes in the soil biomass during extended incubation supports the omission of biomass values from biogeochemical models.  相似文献   

7.
长期有机物循环利用对红壤稻田土壤供磷能力的影响   总被引：6，自引：3，他引：3  
采用盆栽试验,研究了长期不同施肥处理定位试验土壤供磷能力的差异,并从土壤磷素平衡、全磷、有机磷、Olsen-P和MB-P的含量的变化等方面探索了导致供磷能力差异的原因。结果表明,长期施用磷肥能显著提高土壤的供磷能力,其中以有机物循环利用配合磷肥施用处理土壤的供磷量能力最高,植株平均吸磷量是长期不施磷肥处理的3.5倍,比长期施用磷肥处理平均高出59.8%。长期单施氮肥导致土壤供磷能力衰竭,植株总吸磷量比长期不施肥还低17.2%,单一有机物循环利用和配施N肥植株总吸磷量比长期不施肥分别高80.3%和40.2%。有机物循环利用能明显提高土壤微生物对磷素的固持量,土壤微生物对无机磷的利用可能是其向有效磷转化的关键途径。磷肥配合系统内有机物循环利用,是提高红壤稻田土壤供磷能力的有效施肥模式。  相似文献   

8.
不同施肥制度对稻田土壤微生物的影响研究   总被引：20，自引：0，他引：20  
湖南省进行了18年土壤肥力与施肥效益监测的长期定位试验,就3个定位点不同施肥制度对稻田土壤微生物的影响进行了研究。结果表明,长期施肥土壤的有机质含量与细菌总数及放线菌总数之间无直接相关,土壤有机质含量的高低并不能作为评价土壤微生物多少的指标;真菌数量可反映土壤的肥力水平,土壤真菌数量可用来评价不同施肥制度的效果。施用30%有机肥和习惯施肥处理的氮素养分和硝化细菌数量较多,硝化细菌数量与有机质含量也无相关;长期施用大量有机肥(60%)的土壤反硝化细菌数量高于其它施肥处理,这可能与长期使用大量有机肥后土壤氧化还原电位降低有关。硫化细菌和反硫化细菌数量与土壤有机质含量无直接相关性,施肥可影响土壤硫素的供应。施用单质氮肥多的土壤,氨化细菌数量较多,而有机肥施用量高的土壤,氨化细菌数量较少。无肥区的土壤微生物活度最低,60%有机肥处理的土壤微生物活度最高。土壤有机质含量高,土壤微生物活度也高,两者呈正相关。保持农业土壤的健康要从多方面来进行联合调控,而不是单纯的采用某一农业技术来进行。  相似文献   

9.
 The effects of growing trees in combination with field crops on soil organic matter, microbial biomass C, basal respiration and dehydrogenase and alkaline phosphatase activities were studied in soils under a 12-year-old Dalbergia sissoo (a N2-fixing tree) plantation intercropped with a wheat (Triticum aestivum) – cowpea (Vigna sinensis) cropping sequence. The inputs of organic matter through D. sissoo leaf litter increased and crop roots decreased with the increase in tree density. Higher organic C and total N, microbial biomass C, basal soil respiration and activities of dehydrogenase and alkaline phosphatase were observed in treatments with tree-crop combination than in the treatment without trees. Soil organic matter, microbial biomass C and soil enzyme activities increased with the decrease in the spacing of the D. sissoo plantation. The results indicate that adoption of the agroforestry practices led to an improved organic matter status of the soil, which is also reflected in the increased nutrient pool and microbial activities necessary for long-term productivity of the soil. However, tree spacing should be properly maintained to minimize the effects of shading on the intercrops. Received: 21 February 1997  相似文献   

10.
To improve soil fertility, efforts need to be made to increase soil organic matter content. Conventional farming practice generally leads to a reduction of soil organic matter. This study compared inorganic and organic fertilisers in a crop rotation system over two cultivation cycles: first crop broad bean (Vicia faba L.) and second crop mixed cropped melon-water melon (Cucumis melo-Citrullus vulgaris) under semi-arid conditions. Total organic carbon (TOC), Kjeldahl-N, available-P, microbial biomass C (Cmic), and N (Nmic), soil respiration and enzymatic activities (protease, urease, and alkaline phosphatase) were determined in soils between the fourth and sixth year of management comparison. The metabolic quotient (qCO2), the Cmic/Nmic ratio, and the Cmic/TOC ratio were also calculated. Organic management resulted in significant increases in TOC and Kjeldahl-N, available-P, soil respiration, microbial biomass, and enzymatic activities compared with those found under conventional management. Crop yield was greater from organic than conventional fertilizer. The qCO2 showed a progressive increase for both treatments during the study, although qCO2 was greater with conventional than organic fertilizer. In both treatments, an increase in the Cmic/Nmic ratio from first to second crop cycle was observed, indicating a change in the microbial populations. Biochemical properties were positively correlated (p < 0.01) with TOC and nutrient content. These results indicated that organic management positively affected soil organic matter content, thus improving soil quality and productivity.  相似文献   

11.
Soil in short-term crop rotation systems (STCR) is still in the initial development stage of farmland soil, whereas after long-term crop rotation treatment (LTCR), soil properties are significantly different. This study compares STCR (4 years) and LTCR (30 years) rice-rice-fallow, rice-rice-rape rotation practices under the same soil type background and management system. To reveal ecosystem mechanisms within soils and their effects on rice yield following LTCR, we analyzed the physical, chemical, and microbiological properties of soils with different rotations and rotation times. Relative to STCR, LTCR significantly reduced soil water-stable aggregate (WSA) content in the <?0.053-mm range, while >?2 mm WSA content significantly increased. Soil organic matter increased in fields under LTCR, mainly in >?2 mm, 2–0.25 mm, and <?0.053 mm soil WSA in 0–10 cm soil layer. LTCR was associated with significantly increased total soil organic matter, at the same time being associated with increasing the amount of active organic matter in the 0–20 cm soil layer. The two crop rotation regimes significantly differed in soil aggregate composition as well as in soil N and P, microbial biomass, and community composition. Relative to STCR, LTCR field soils had significantly higher soil organic matter, active organic matter content, soil enzyme activities, and overall microbial biomass, while soil WSA and microbial community composition was significantly different. Our results demonstrate that LTCR could significantly improve soil quality and rice yield and suggest that length of rotation time and rice-rice-rape rotation are critical factors for the development of green agriculture.  相似文献   

12.
2,4-Dichlorophenoxyacetic acid butyl ester (2,4-D butyl ester) is extensively applied for weed control in cultivation fields in China, but its effect on soil microbial community remains obscure. This study investigated the microbial response to 2,4-D butyl ester application at different concentrations (CK, 10, 100 and 1000 μg g?1) in the soils with two fertility levels, using soil dilution plate method and phospholipid fatty acid (PLFA) analysis. Culturable microorganisms were affected by the herbicide in both soils, particularly at the higher concentration. After treating soil with 100 μg g?1 herbicide, culturable bacteria and actinomycetes were significantly higher, compared to other treatments. Treatment of soil with 1000 μg g?1 2,4-D butyl ester caused a decline in culturable microbial counts, with the exception of fungal numbers, which increased over the incubation time. PLFA profiles showed that fatty acids for Gram-negative (GN) bacteria, Gram-positive (GP) bacteria, total bacteria and total fungi, as well as total PLFAs, varied with herbicide concentration for both soil samples. As herbicide concentration increased, the GN/GP ratio decreased dramatically in the two soils. The higher stress level was in the treatments with high concentrations of herbicide (1000 μg g?1) for both soils. Principal component analysis of PLFAs showed that the addition of 2,4-D butyl ester significantly shifted the microbial community structure in the two soils. These results showed that the herbicide 2,4-D butyl ester might have substantial effects on microbial population and microbial community structure in agricultural soils. In particular, the effects of 2,4-D butyl ester were greater in soil with low organic matter and fertility level than in soil with high organic matter and fertility level.  相似文献   

13.
Microbial activity of arable soils in Lower Saxony, Germany. II. Soil characterization by microbial activities Field trials were carried out on six soil types in Lower Saxony and were supported by glasshouse and laboratory trials. Soils were sampled frequently to monitor biomass-related and overall microbial activities as well as the activity of soil enzymes. Similar soils showed comparable activity patterns. Crop plants and, especially, the herbicide ‘Flüssig Herbogil’ (dinoterb) affected some microbial functions. Activity patterns can be used in long-term trials to characterize soil types and agricultural practices. In addition, they may be useful in short-time trials to identify ecological and ecotoxicological effects.  相似文献   

14.
不同耕作方式对土壤有机碳、微生物量及酶活性的影响   总被引：12，自引：2，他引：10  
【目的】依托8年长期(2005~2012)固定道定位试验,研究不同耕作方式对土壤有机碳、土壤微生物量、土壤酶活性在0—90 cm土层的分布特征,为优化中国西北干旱区的耕作方式提供理论依据。【方法】试验包括固定道垄作(PRB)、固定道平作(PFT)与传统耕作(CT)三种耕作模式下的土壤有机碳土壤总有机碳(TOC)、颗粒有机碳(POC)、土壤微生物量碳(MBC)、土壤微生物量氮(MBN)、土壤微生物量磷(MBP)、蔗糖酶、过氧化氢酶、脲酶及小麦产量进行了测定和分析。【结果】在0—90 cm土层,不同耕作方式下的TOC、POC、MBC、MBN、MBP、蔗糖酶活性、脲酶活性均随着土层的增加呈下降趋势,过氧化氢酶活性呈先下降后增大的分布特征;在0—60 cm,固定道保护性耕作能够显著增加心土层作物生长带土壤有机碳储量,有机碳储量大小为PRBPFTCT;PRB、PFT较CT可以显著增加0—10 cm作物生长带TOC、POC、MBC、MBN、MBP含量、蔗糖酶、脲酶活性,其大小为PRBPFTCT;耕作方式对过氧化氢酶活性影响不显著;TOC、POC、MBC、MBN、MBP、蔗糖酶活性、脲酶活性、过氧化氢酶活性之间均达到了显著或极显著相关。【结论】PRB较PFT、CT能够提高耕作层(0—10 cm)土壤有机碳含量、土壤微生物量、土壤酶活性, 增加作物产量, 增大0—60 cm土层有机碳储量,耕作方式(PRB、PFT及CT)对10 cm以下土层土壤环境改善作用不明显。  相似文献   

15.
城郊土壤不透水表面有土壤机碳转化及其相关性质的研究   总被引：2，自引：0，他引：2  
Installation of impervious surface in urban area prevents the exchange of material and energy between soil and other environmental counterparts, thereby resulting in negative effects on soil function and urban environment. Soil samples were collected at 0-20 cm depth in Nanjing City, China, in which seven sites were selected for urban open soils, and fourteen sites with similar parent material were selected for the impervious-covered soils, to examine the effect of impervious surface on soil properties and microbial activities, and to determine the most important soil properties associated with soil organic carbon （SOC） transformation in the urban soils covered by impervious surfaces. Soil organic carbon and water-soluble organic carbon （WSOC） concentrations, potential carbon （C） and nitrogen （N） mineralization rates, basal respiration, and physicochemical properties with respect to C transformation were measured. Installation of impervious surface severely affected soil physicochemical properties and microbial activities, e.g., it significantly decreased total N contents, potential C mineralization and basal respiration rate （P 〈 0.01）, while increased pH, clay and Olsen-P concentrations. Soil organic carbon in the sealed soils at 0-20 cm was 2.35 kg m-2, which was significantly lower than the value of 4.52 kg m-2 in the open soils （P 〈 0.05）. Canonical correlation analysis showed WSOC played a major role in determining SOC transformation in the impervious-covered soil, and it was highly correlated with total N content and potential C mineralization rate. These findings demonstrate that installation of impervious surface in urban area, which will result in decreases of SOC and total N concentrations and soil microbial activities, has certain negative consequences for soil fertility and long-term storage of SOC.  相似文献   

16.
长期不同施肥模式下碱性土有效磷对磷盈亏的响应   总被引：2，自引：1，他引：1  
[目的]磷素易于在土壤中固定,碱性土壤更甚,影响着磷肥的肥效和利用效率.研究长期施用磷肥对我国北方碱性土有效磷与磷盈亏的影响,为碱性土地区合理施用磷肥提供理论依据.[方法]本研究是基于河北、北京、山东、天津、河南和山西的6个冬小麦-夏玉米轮作长期定位施肥试验,试验周期为1991-2011年.所有定位施肥试验均设有不施磷...  相似文献   

17.
Summary Microbial biomass C and N respond rapidly to changes in tillage and soil management. The ratio of biomass C to total organic C and the ratio of mineral N flush to total N were determined in the surface layer (0–5 cm) of low-clay (8–10%), fine sandy loam, Podzolic soils subjected to a range of reduced tillage (direct drilling, chisel ploughing, shallow tillage) experiments of 3–5 years' duration. Organic matter dynamics in the tillage experiments were compared to long-term conditions in several grassland sites established on the same soil type for 10–40 years. Microbial biomass C levels in the grassland soils, reduced tillage, and mouldboard ploughing treatments were 561, 250, and 155 g g-1 soil, respectively. In all the systems, microbial biomass C was related to organic C (r=0.86), while the mineral N flush was related to total N (r=0.84). The average proportion of organic C in the biomass of the reduced tillage soils (1.2) was higher than in the ploughed soils (0.8) but similar to that in the grassland soils (1.3). Reduced tillage increased the average ratio of mineral N flush to total soil N to 1.9, compared to 1.3 in the ploughed soils. The same ratio was 1.8 in the grassland soils. Regression analysis of microbial biomass C and percent organic C in the microbial biomass showed a steeper slope for the tillage soils than the grassland sites, indicating that reduced tillage increased the microbial biomass level per unit soil organic C. The proportion of organic matter in the microbial biomass suggests a shift in organic matter equilibrium in the reduced tillage soils towards a rapid, tillage-induced, accumulation of organic matter in the surface layer.  相似文献   

18.
In mountain areas, water erosion plays an important role on soil structure and can strongly affect its functions. Suitable management practices, namely choice of crops, may be able to improve aggregate stability and reduce soil loss by surface runoff. To study the effects of cover crops on aggregation in mountain soils, we investigated a soil planted with wheat (site C) compared with two soils under alfalfa (sites A and B). All investigated soils were Typic Ustorthents. The soil aggregates were isolated by a physical‐functional procedure defined on the basis of aggregate resistance against specific breakdown mechanisms such as slaking and water abrasion. One year after alfalfa plantation, its effect on wet aggregate stability and the amount of organic carbon (OC) and nutrients retained by the isolated aggregates were measured. In the less degraded soils (site B), organic matter decomposition was stimulated, and after 1 year, the total OC (TOC) and TOC/N declined. Consequently, a reduction in aggregate stability and ability to preserve elements occurred, as shown by the decrease of wet aggregate stability indexes and total nutrients (e.g. N, P and K) retained by stable aggregates. In more degraded soil (site A), alfalfa generally positively affected the investigated properties. The effect of alfalfa in the studied crop rotation depends on the degree of land degradation due to water erosion. As regards the investigated breakdown mechanisms, our data showed that the aggregate resistance to water abrasion was related to TOC, while the resistance against fast wetting breakdown is correlated to the microbial biomass. Copyright © 2017 John Wiley & Sons, Ltd.  相似文献   

19.
From 1969 to 1974, two experimental fields were studied in terms of their soil microbial and biochemical characteristics in relation to soil management practice and crop yields. Of the various microbial and biochemical properties measured soil phosphatase, saccharase, β-glucosidase and urease activities, N mineralization and soil respiration were found valuable to characterize the soils. These characteristics increased with increasing soil organic matter, clay and CaCO3 content. They also revealed a strong effect of organic fertilization. Multiple regression analyses indicated that the alkalinity and the humus content of the soil largely determined the magnitude of these characteristics. The regression analyses also showed that the yields of winter wheat were related positively to phosphatase activity while the sugarbeet yields were related negatively to soil urease activity.  相似文献   

20.
As a major attribute of soil quality, organic matter is responsive to agricultural land use practices including tillage. A study was initiated in eastern Canada to characterize changes in the masses of organic C and total N, and organic matter fractions in forested and adjacent cultivated or forage sites. Generally, the cultivated and forage sites had denser soil profiles than the forest sites. Based on an equivalent soil mass, to accommodate differences in soil bulk density, the paired forest and cultivated sites showed that cultivation decreased the mass of organic C (35%) and total N (10%) in the soil profile of the Podzolic soils, but increased organic C (25%) and total N (37%) in the Brunisolic (Cambisol) and Gleysolic soils. For the Podzolic soils, use of forages increased soil stored organic C and N by 55% and 35%, respectively. Organic C fractions were mainly of significance in the A horizon. Soil microbial biomass C was greater in the forested, compared to the cultivated soil, but the proportion of soil organic C as microbial biomass C (1.3% to 1.6%) was similar. The proportion, however, was greater (2.1%) for the forage soil, compared to the corresponding cultivated (1.3%) soil, suggesting that organic C was continuing to increase under the former. The relatively large proportion (19%) of organic C found in the light fraction of forest soils in the A horizon was decreased (up to 70%) by cultivation. In contrast, the proportion of macro-organic C present in the soil sand fraction was not greatly influenced by cultivation. Overall, soils in eastern Canada have a relatively large potential to store organic matter. The study illustrates the importance of soil type and cultivation interactions for maintenance of soil organic matter storage, and the positive influence of forages in this regard in agroecosystems.  相似文献   

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