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1.
Growing areas under transgenic crops have created a concern over their possible adverse impact on the soil ecosystem. This study evaluated the effect of Bt-cotton based cropping systems on soil microbial and biochemical activities and their functional relationships with active soil carbon pools in Vertisols of central India (Nagpur, Maharastra, during 2012–2013). Culturable groups of soil microflora, enzymatic activities and active pools of soil carbon were measured under different Bt-cotton based cropping systems (e.g. cotton-soybean, cotton-redgram, cotton-wheat, cotton-vegetables and cotton-fallow). Significantly higher counts of soil heterotrophs (5.7–7.9 log cfu g?1 soil), aerobic N-fixer (3.9–5.4 log cfu g?1 soil) and P-solubilizer (2.5?3.0 log cfu g?1 soil) were recorded in Bt-cotton soils. Similarly, soil enzymatic activities, viz. dehydrogenase (16.6–22.67 µg TPF g?1 h?1), alkaline phosphatase (240–253 µg PNP g?1 h?1) and fluorescein di-acetate hydrolysis (14.6–18.0 µg fluorescein g?1 h?1), were significantly higher under Bt-cotton-soybean system than other Bt- and non-Bt-cotton based systems in all crop growth stages. The growth stage-wise order of soil microbiological activities were: boll development > harvest > vegetative stage. Significant correlations were observed between microbiological activities and active carbon pools in the rhizosphere soil. The findings indicated no adverse effect of Bt-cotton on soil biological properties.  相似文献   

2.
This study investigates the effect of conjoint use of bio-organics (biofertilizers + crop residues + FYM) and chemical fertilizers on yield, physical–chemical and microbial properties of soil in a ‘French bean–cauliflower’-based cropping system of mid hills of the north-western Himalayan Region (NWHR) of India. Conjoint bio-organics at varied levels of NPK chemical fertilizers increased yield of ‘cauliflower’ over corresponding single application. Incorporation of crop residues with 75% of the recommended NPK application resulted in the highest yield (19 t ha?1). Conjoint use of bio-organics produced a yield (15.65 t ha?1), which was statistically on a par with 75% of the recommended NPK application alone. This indicated a saving of 75% NPK chemical fertilizers. In the case of ‘French bean’, the effect was non-significant. The results also showed significant higher soil available N (351.3 kg ha?1) under 75% NPK + biofertilizers, whereas the highest soil available K (268.3 kg ha?1) was recorded under 75% NPK + crop residues. Lowest bulk density (1.03 Mg m?3), highest water holding capacity (36.5%), soil organic matter (10.6 g kg?1), bacterial (4.13 × 107 cfu g?1) and fungal (6.3 × 107 cfu g?1) counts were recorded under sole application of bio-organics. According to our study, we concluded that the combination of NPK fertilizers and bio-organics increased yield except French bean, soil available N, K and saved chemical fertilizers under ‘French bean–cauliflower’-based cropping system.  相似文献   

3.
Root samples of 11 non-cultivated monocotyledonous and 7 dicotyledonous species taken during a wet summer had low mean nitrogenase activities of 10.2 and 7.1 nmol C2H4·g?1 DW·h?1 after preincubation at pO2 0.02, respectively. Maxima of 139–169 nmol·g?1·h?1 were observed with Agrostis vulgaris and Agropyron repens on a sandy soil poor in Corg. Three of 6 early, but none of 4 late fodder maize cultivars had a very low activity up to 0.5 nmol·g?1h?1. Oat, rye and wheat roots from plots with organic or mineral N fertilizers had activities between 1.3 and 7.3 nmol·g?1h?1 at flowering, which were not correlated with their Azospirillum populations (102-107·g?1 after preincubation). Winter wheat and barley roots given 0, 40, 80 and 120 kg. ha?1 NH4NO3-N in 0–3 applications had mean activities of 0.08, 4.06, 0.09 and 0.08 nmol or 1.77, 2.67, 0.36 and 0.23 nmol C2H4g?1·h?1 after flowering, respectively. An appreciable part of this activity could be removed by root washing. In preincubated rhizosphere soil of wheat and barley populations of N2-fixing, facultative anaerobic Klebsiella and Enterobacter spp. were 10–100 times higher than those of Azospirillum sp., both being higher in O N than in 80 kg N·ha?1 trials.  相似文献   

4.
Radioactive substances were released into the environment after the nuclear accident at the Fukushima Daiichi Nuclear Power Station; this led to the contamination of the soil at Fukushima Prefecture. Mixing of organic matter with soil during plowing is known to influence radiocesium (134Cs and 137Cs) absorption by crops. However, the effect of mixing organic matter polluted by radioactive substances during plowing on radiocesium absorption by plants is not yet known. The aim of this study was to investigate the effect on the radiocesium absorption by komatsuna (Brassica rapa L. var. perviridis) cultivated in a 45-L container containing Andosol (14,300 Bq kg?1) or Gray Lowland soil (33,500 Bq kg?1) mixed with polluted wheat (Triticum aestivum L. Thell.) straw (2080 Bq kg?1). The radiocesium concentration of the plants and the soil and the amount of exchangeable radiocesium in the soil were determined using a germanium semiconductor. The transfer of radiocesium from the soil to plants decreased by 53 and 27% in Andosol and Gray Lowland soil, respectively, after the application of 10 t ha?1 polluted wheat straw. This reduction in the level of radiocesium transfer might be attributed to potassium contained in the wheat straw, which might compete with cesium during membrane transport and thereby block the transport of cesium from the soil solution to the roots and from the roots to the shoots. Alternatively, the applied wheat straw probably absorbed radiocesium and decreased the amount of exchangeable radiocesium in the soil. Our findings suggest that the mixing of polluted wheat straw with contaminated soil might influence the absorption of radiocesium content by agricultural products. Further studies are warranted to determine the long-term effects of the application of polluted wheat straw on the rate of radiocesium transfer to crops.  相似文献   

5.
The potential of Nostoc 9v for improving the nitrogen (N)2–fixing capacity and nutrient status of semi‐arid soils from Tanzania, Zimbabwe, and South Africa was studied in a laboratory experiment. Nostoc 9v was inoculated on nonsterilized and sterilized soils. Inoculum rates were 2.5 mg dry biomass g?1 soil and 5 mg dry biomass g?1 soil. The soils were incubated for 3 months at 27 °C under 22 W m2 illumination with a photoperiod of 16 h light and 8 h dark. The moisture was maintained at 60% of field capacity. In all soils, Nostoc 9v proliferated and colonized the soil surfaces very quickly and was tolerant to acidity and low nutrient availability. Cyanobacteria promoted soil N2 fixation and had a pronounced effect on total soil organic carbon (SOC), which increased by 30–100%. Total N also increased, but the enrichment was, in most soils, comparatively lower than for carbon (C). Nitrate and ammonium concentrations, in contrast, decreased in all the soils studied. Increases in the concentration of available macronutrients were produced in most soils and treatments, ranging from 3 to 20 mg phosphorus (P) kg?1 soil, from 5 to 58 mg potassium (K) kg?1 soil, from 4 to 285 mg calcium (Ca) kg?1, and from 12 to 90 mg magnesium (Mg) kg?1 soil. Positive effects on the levels of available manganese (Mn) and zinc (Zn) were also observed.  相似文献   

6.
Boron (B) is an essential microelement, which is necessary for reproductive organs including pollen tube formation in wheat (Triticum aestivum L.), and flowering and boll formation in cotton (Gossypium hirsutum L.) The study was associated with wheat-cotton rotation in 80 farm fields, belonging to different soil series, in four districts of cotton belt of Punjab, Pakistan to assess concentrations of extractable B in soils [0.05 M hydrochloric acid (HCl) extractable B], and added fertilizer B and their relationship to some soil physico-chemical properties [pH, organic matter (OM), calcium carbonate (CaCO3) and clay content], yields and total B concentrations in wheat and cotton plants. All soils had alkaline pH (7.45 to 8.55), high CaCO3 content (2.14 to 8.65%), less than 1.0% OM (0.33 to 0.99%), low plant available-P (Olsen P less than 8 mg kg?1 soil) and medium ammonium acetate extractable potassium (K) (< 200 mg K kg?1 soil). Of the 80 soil samples, 65 samples (81%) were low in available B (<0.45 mg B kg?1, ranging from 0.11 to 0.43 mg B kg?1) Of the corresponding 80 plant samples, leaves B concentrations were below critical levels (<10 mg B kg?1 for wheat; <30 mg B kg?1 for cotton) for all the tested samples for wheat and cotton. The regression analysis between plant total B concentrations and soil extractable B concentrations showed strong linear positive relationships for both wheat (R2 = 0.509***, significant at P <0.001) and cotton (R2 = 0.525***, significant at P <0.001). Further regression analysis between extractable soil B and wheat grain yield as well as between wheat leaves total B and wheat grain yield also depicted strong linear relationships (R2 = 0.76 and 0.42, respectively). Boron fertilizer demonstration plots laid out at farmers’ fields low in extractable B, in each district not only enhanced grain yields of wheat crop but also contributed a significant increase towards seed cotton yield of succeeding cotton crop through residual B effect. In conclusion, the findings suggest that many soils in the cotton belt of Punjab may be low in extractable B for wheat and cotton, especially when these crops are grown on low OM soils with high CaCO3 content.  相似文献   

7.
Abstract

Efficient nutrient and water use are two important considerations to obtain good harvests of wheat. This necessitates the development of an effective nutrient management technique that not only increases yield, but simultaneously can save nutrient and water use. In this context, a field experiment was conducted at Indian Agricultural Research Institute, New Delhi, India to evaluate the residual effect of sesbania and rice bean (in-situ), subabul (ex-situ) green manuring and Zinc (Zn) fertilization, using chelated Zn-ethylenediaminetetraacetic acid (Zn-EDTA) on nutrient use, yields and water productivity of wheat under rice–wheat cropping system. Among residual effects of green manure crops and Zn fertilization, sesbania and foliar spray of 0.5% chelated Zn-EDTA at 20, 40, 60 and 80 days after sowing (DAS) recorded significantly higher nutrient content and uptake and yields than other green manure crops and Zn treatments. Residual effect of sesbania saved about 46.5?×?103 and 30.5?×?103 L irrigation water per tonne of wheat over subabul and rice bean, respectively. Foliar spray of 0.5% chelated Zn-EDTA at 20, 40, 60 and 80 DAS saved about 55.5?×?103, 47?×?103 and 13?×?103 L irrigation water per tonne wheat over residual effect of 5?kg Zn ha?1 through chelated Zn-EDTA as soil application, 2.5?kg Zn ha?1 through chelated Zn-EDTA as soil application + 1 foliar spray of 0.5% chelated Zn-EDTA at flowering and foliar spray of 0.5% chelated Zn-EDTA at active tillering?+?flowering?+?grain filling, respectively. Correlation analysis showed positive correlation between Zn uptake and grain yield.  相似文献   

8.
Wheat (Triticum aestivum L.) residues and nitrogen (N) management are the major problems in the southern part of Iran where irrigated wheat–cotton (Gossypium hirsutum L.)–wheat rotation is a common practice. A 2-year (2009–2011) field experiment was conducted as a split plot design with four replications at a cotton field (Darab), Fars Province, Iran, to determine the influence of different rates of wheat residue (0%, 25%, 50%, and 75%) incorporation and N rates (150, 200, 300, and 400 kg ha?1) on weed suppression, yield, and yield components of cotton. Results showed that a higher residue incorporation and a lower N rate improved weed suppression in both years. For treatments receiving 150 kg N ha?1 and 75% of wheat residues (2250 kg ha?1), weed biomass and density were significantly lower compared to treatments receiving 400 kg N ha?1. The highest cotton lint yield (about 2400–2700 kg ha?1) was obtained by 300 kg N ha?1 in the absence of residue application, in both years. Incorporation of 25% of wheat residue (750 kg ha?1) and application of 300 kg N ha?1 are recommended to guarantee an optimum level of cotton lint yield and weed suppression in a wheat–cotton–wheat rotation in this region.  相似文献   

9.
Abstract

Zinc (Zn) deficiency is a widespread micronutrient disorder in crops grown in calcareous soils; therefore, we conducted a nutrient indexing of farmer‐grown rainfed wheat (Triticum aestivum, cv. Pak‐81) in 1.82 Mha Potohar plateau of Pakistan by sampling up to 30 cm tall whole shoots and associated soils. The crop was Zn deficient in more than 80% of the sampled fields, and a good agreement existed between plant Zn concentration and surface soil AB‐DTPA Zn content (r=0.52; p≤0.01). Contour maps of the sampled areas, prepared by geostatistical analysis techniques and computer graphics, delineated areas of Zn deficiency and, thus, would help focus future research and development. In two field experiments on rainfed wheat grown in alkaline Zn‐deficient Typic Haplustalfs (AB‐DTPA Zn, 0.49–0.52 mg kg?1), soil‐applied Zn increased grain yield up to 12% over control. Fertilizer requirement for near‐maximum wheat grain yield was 2.0 kg Zn ha?1, with a VCR of 4∶1. Zinc content in mature grain was a good indicator of soil Zn availability status, and plant tissue critical Zn concentration ranges appear to be 16–20 mg kg?1 in young whole shoots, 12–16 mg kg?1 in flag leaves, and 20–24 mg Zn kg?1 in mature grains.  相似文献   

10.
High nitrogen fixing, phosphate solubilizing, phytohormones producing isolates of Azotobacter, Azospirillum, Acetobacter and Pseudomonas were used as inoculants for cotton. Important cultures were selected on the basis of their effect on root/shoot length and chemotactic behaviour. Selected bioinoculants were earlier tested for their beneficial properties like nitrogen fixation (ARA), ammonia excretion, IAA production etc. These bio-inoculants were further tested for phosphate solubilization property. Various chosen strains were tested with Desi (HD 123) and American (H 1098) cotton under field conditions (as for wheat). Plant height and boll weight were determined at the time of harvesting whereas survival rate of inoculated bacteria was determined after 30, 80 and 130 days respectively. In the year 2000–01, on the basis of boll number and boll weight plant?1 AVK 51 (36; 76.2?g plant?1), HT 57 (27; 56.9?g plant?1), AC18 (33; 61.5?g plant?1), Ala 27 (36; 61.4?g plant?1) and Pseudomonas (34; 71.3?g plant?1) were identified as significant both for American and desi cotton varieties. Highest survival rate was observed with Mac 68 (33.4 × 105) followed by HT54 (31.5 × 105) after 30 days of sowing, which decreased after 80 days and remained constant up to 130 days. This trend was observed with all the cultures. Similar results were observed in 2001–02. 25?kg ha?1 N saving was observed with A. chroococcum (AVK51) bioinoculant for cotton crop.  相似文献   

11.
Drainage water from agricultural fields with applied manure can degrade the bacterial quality of surface and groundwater. The impact of conventional tillage (CT) and zero tillage (ZT) practices on Escherichia coli (E.coli) discharge through artificially drained soils is not well understood. Consequently, two field trials were conducted during 2002–2004. The first trial involved fall applications of beef manure while the second involved spring applications of dairy manure. Both surface and subsurface drainage water were monitored in the first trial while only subsurface drainage water was monitored in the second. Under fall applied beef manure (trial 1), no differences (p?>?0.05) were observed in E.coli concentrations (cfu/100 ml) in combined drainage water under both tillage systems. However, during 2003–2004, subsurface drainage water under ZT had higher E.coli concentrations and loads than drainage water under CT. When the combined (surface + subsurface) annual E.coli loads were considered, CT loads were greater than ZT during 2002–2003 with an opposite situation during 2003–2004. Overall, annual E.coli loads were similar under ZT (4.7?×?1010 cfu/ha) and CT (4.8?×?1010 cfu/ha). Spring dairy manure application (trial 2) produced significant (p?>?0.03) tillage effect on E.coli loads in subsurface drainage water only during the second year. During the study period, ZT plots (1.55?×?1010 cfu/ha) discharged 5× more E.coli than CT (0.23?×?1010 cfu/ha). A longer duration of ZT practices resulted in higher subsurface flow volumes and subsequently greater loads of E.coli discharge in both trials.  相似文献   

12.
Cotton plants that are continuously subjected to monoculture suffer greatly from Verticillium wilt disease. The application of a novel bioorganic fertilizer (BOF) consisting of organic fertilizer combined with the antagonistic Bacillus subtilis strain HJ5 significantly suppressed Verticillium wilt of cotton. The disease incidence rates in soils that were treated with BOF (1 %, w/w) in the nursery stage, in the transplanted soil stage or in both stages, decreased by 42.9 %, 57.1 %, and 88.0 %, respectively, compared with controls. B. subtilis HJ5 was tagged with a plasmid-borne gfp gene encoding the green fluorescent protein to investigate its colonization behavior on cotton root surfaces. The results indicated that B. subtilis HJ5 predominantly colonizes the elongation and differentiation zones of the roots and forms micro-colonies in hydroponic and soil systems. The population of B. subtilis HJ5 in the rhizosphere and on cotton roots was also monitored. The number of B. subtilis HJ5 cells on the root surface reached a peak value of approximately 107 cfu per gram of roots 3 days after exposure of the cotton seedlings to the bacteria. Probably the colonization of B. subtilis HJ5 on cotton roots is one of the mechanisms involved in protecting cotton plants from fungal infection.  相似文献   

13.
Long-term effects of chemical fertilizers and farmyard manure (FYM) in rice (Oryza sativa)–wheat (Triticum aestivum) cropping system were monitored for two consecutive years after 38 and 39 years on productivity and soil biological properties of Mollisols. The study encompasses varying chemical fertilizer levels of optimum fertilizer rate (120, 26 and 37 kg ha?1 N, P and K, respectively) for both the crops. The treatments were application of 50% NPK, 100% NPK, 150% NPK, 100% NPK + hand weeding (HW), 100% NPK + Zn, 100% NP, 100% N, 100% NPK + 15 t FYM ha?1, 100% NPK(-S) and unfertilized control. The rice and wheat yields were highest with 100% NPK + 15 t FYM ha?1. This treatment also gave maximum and significantly more counts of bacteria, fungi and actinomycetes in soil than all the other treatments after crop harvest. The soil microbial biomass C (410.0 and 407.5 µg g?1) and N (44.53 and 48.30 µg g?1) after rice and wheat, respectively, were highest with 100% NPK + 15 t FYM ha?1, which were significantly higher over all the other treatments. The activities of soil enzymes like dehydrogenase, acid and alkaline phosphatase, arylsulphatase and urease and CO2 evolution rate with 100% NPK + 15 t FYM ha?1 were also found significantly higher over the other treatments. Fertilizer treatments with 100% NPK and 150% NPK were comparable and significantly better than application of 50% NPK, 100% N, 100% NP and 100% NPK(-S) in various studied soil biological properties. Integrated use of 100% NPK with FYM sustained the higher yields and soil biological properties under rice–wheat cropping system in Mollisols. Application of Zn and hand weeding with 100% NPK were found better over 100% NPK alone in rice and wheat productivity. Imbalanced use of chemical fertilizers had the harmful effect on soil biological health.  相似文献   

14.
Abstract

An experiment was conducted to substitute mineral fertilizers with biofertilizers in strawberry to work out the yield, quality of strawberry and soil fertility. A 25% substitution of mineral fertilizer with biofertilizer increased the number of fruits/plant along with improving Juice content (89.55%), Total soluble solids (10.35°B), total sugar (6.69%), ascorbic acid (43.80?mg 100?g?1), anthocyanin content (81.05?mg 100?g?1), total phenol (5.97?mg Gallic acid equiv. g?1), flavonoids (0.12?mg g?1) and antioxidant capacity (2.13?µmol. Trolox equiv. 100?g?1). The available N and K content in post-harvest soils were improved significantly with 75% RDF + Azospirillium @ 2?g plant?1 + PSB @ 2?g plant?1 + topdressing of 25% K treatments (200.10 and 211.70?kg ha?1, respectively). Viable count of soil microorganisms (Bacteria, actinomycetes and fungi) was also estimated maximum (4066, 190 and 11.33?×?104 cfu g?1?dry soil, respectively) with substitution of 25% of mineral fertilizer either with Azotobacter or Azospirillum.  相似文献   

15.
ABSTRACT

We studied the effect of integrated nutrient management (INM) combinations on supplement of plant nutrient for quantitative and qualitative fruit production in sapota. Thus, 17 combinations of INM practices were evaluated on fruit yield of sapota and nutrient availability in a Vertisol of Chambal region, India. The results demonstrated that almost all treatment combinations comprised of recommended dose of fertilizer (RDF), i.e. 1,000:500:500 g NPK plant?1 with application of organic and inorganic sources of nutrients had a significant effect on the fruit yield of sapota, soil microbial biomass, NPK content of leaf, fruit and soil over control (T1). Among different treatments, application of 2/3rd part of RDF + 50 kg FYM + 250 g Azospirillum + 250 g Azotobacter plant?1 (T11) significantly enhanced the number of fruits plant?1 (327.88), yield plant?1 (29.03 kg) and yield ha?1 (4.52 t). However, the soil microbial count of fungi (8.89 cfu g?1 soil), bacteria (11.19 cfu g?1 soil) and actinomycetes (5.60 cfu g?1 soil) at fruit harvest was higher under the 2/3 of RDF +10 kg vermicompost + 250 g Azospirillum + 250 g Azotobacter plant?1 (T15). The leaf nitrogen content (N, 2.03%) was higher in T15, while phosphorus (P, 0.28%) and potassium (K, 1.80%) content were higher in T11. It is evident that treatment T11 increased fruit yield by 32% in Sapota cv. Kalipatti compared to control. Therefore, combined application of nutrient sources proved not only beneficial for enhancing fruit yield of sapota but also sustaining soil health in Chambal region of south-eastern Rajasthan.  相似文献   

16.
A sandy soil was amended with different types of sewage sludge (digested, dried, and composted) and pig slurry. The composted sludges displayed higher organic‐matter stability (39–45%) than only digested sludge (26–39%) or digested + dried sludge (23–32%). The microbial biomass of the dried sludge was undetectable. Digested and composted sludges and pig slurry displayed microbial biomasses (12492–13887 µg g?1, 1221–2050 µg g?1, and 5511 µg g?1, respectively) greater than the soil (108 µg g?1). The wastes were applied at seven doses, ranging from 10 to 900 g kg?1. Soils were incubated for 28 days. Substrate‐induced respiration (SIR) was measured for 12 consecutive hours on day 1 and on day 28. The results showed that SIR increased with the dose of organic amendment. However, SIR decreased when moderate doses of pig slurry or high doses of digested + dried sludge were tested. The possibility of using this inhibition as an ecotoxicological indicator is discussed.  相似文献   

17.
Abstract

Characterization of methanotrophs isolated from paddy soils and rice (Oryza sativa) roots was investigated in the present study. The number of methanotrophs in root homogenates of the rice cultivar Mutsuhomare was 4.9 × 107 most-probable-number (MPN) g?1 dry roots, in Yumeakari it was 2.0 × 108 MPN g?1 dry roots and in Kirara it was 4.6 × 107 MPN g?1 dry roots. Although bacterial cells were observed infrequently on the surface and in the interiors of roots before incubation, a large number of colonies, measuring 0.5–5 mm in diameter, were observed on the sterilized roots after incubation on nitrate mineral agar plates under methane in air. In particular, a large number of colonies were observed at the emergence sites of lateral roots and root hairs. Strains MD5-1 and M1 were isolated from the roots of Mutsuhomare and strain R62 was isolated from the root homogenate of Yumeakari. All isolates were catalase-positive and oxidase-positive, Gram-negative, straight-rod-shaped and curved-rod-shaped bacteria, and formed exospores. The isolates were able to fix nitrogen and grew in the absence of copper. In addition, all were found to be positive for naphthalene-oxidizing activities (corresponding to soluble methane mono-oxygenase activities). Strains MD5-1, M1 and R62 were closely related to Methylosinus sporium. Methanotrophic strains W3-6, SD3-5 and 2-19, isolated previously from paddy field soils, were classified into Methylosinus (W3-6) and Methylocystis (SD3-5 and 2-19) type II methanotrophs. Isolates from the rice roots (MD5-1, R62 and M1) grew logarithmically when casamino acid was used as the nitrogen source; however, the growth of these strains was reduced on the nitrate medium. These strains preferred amino acids over inorganic nitrogen as a nitrogen source for growth.  相似文献   

18.
A long-term field experiment was conducted for 8 years on a Vertisol in central India to assess quantitatively the direct and residual N effects of soybean inoculation with Bradyrhizobium and wheat inoculation with Azotobacter in a soybean–wheat rotation. After cultivation of soybean each year, its aerial residues were removed before growing wheat in the same plots using four N levels (120, 90, 60 and 30 kg ha?1) and Azotobacter inoculation. Inoculation of soybean increased grain yield by 10.1% (180 kg ha?1), but the increase in wheat yields with inoculation was only marginal (5.6%; 278 kg ha?1). There was always a positive balance of soil N after soybean harvest; an average of +28 kg N ha?1 yr?1 in control (nodulated by native rhizobia) plots compared with +41 kg N ha?1 yr?1 in Rhizobium-inoculated plots. Residual and direct effects of Rhizobium and Azotobacter inoculants caused a fertilizer N credit of 30 kg ha?1 in wheat. Application of fertilizers or microbial inoculation favoured the proliferation of rhizobia in crop rhizosphere due to better plant growth. Additional N uptake by inoculation was 14.9 kg N ha?1 by soybean and 20.9 kg N ha?1 by wheat crop, and a gain of +38.0 kg N ha?1 yr?1 to the 0–15 cm soil layer was measured after harvest of wheat. So, total N contribution to crops and soil due to the inoculants was 73.8 kg N ha?1 yr?1 after one soybean–wheat rotation. There was a total N benefit of 13.8 kg N ha?1 yr?1 to the soil due to regular long-term use of microbial inoculants in soybean–wheat rotation.  相似文献   

19.
Abstract

The effect of zinc–phosphorus (Zn‐P) interaction on Zn efficiency of six wheat cultivars was studied. The higher dry matter yields were observed when Zn was applied at 5 µg g?1 soil than with no Zn application. Phosphorus applications also increased dry matter yield up to the application of 25 µg P g?1 soil. The dry matter yield was significantly lower at the P rate of 250 µg g?1 soil. At the Zn‐deficient level, the Zn‐efficient cultivars had higher Zn concentrations in the shoots. Zinc concentrations in all cultivars increased when the P level in the soil was increased from 0 to 25 µg P g?1 soil except for the cv. Durati, in which Zn concentrations decreased with increases in P levels. However, when Zn×P interactions were investigated, it was observed that at a Zn‐deficient level, Zn concentrations in the plant shoot decreased with each higher level of P, and more severe Zn deficiency was observed at P level of 250 µg g?1 soil.  相似文献   

20.
Dry matter (DM) partitioning into root, leaf, stem, shoot dry weight plant?1 response in four cool season C3-cereals viz. wheat (Triticum aestivum L.), rye (Secale cereale L.), barley (Hordeum vulgare L.) and oats (Avena sativa L.) was investigated at 30, 60 and 90 days after emergence (DAE) under eight nitrogen, phosphorus and potassium (NPK) sources: S1 = 20-20-20, S2 = 20-27-5, S3 = 7-22-8, S4 = 10-10-10-20S, S5 = 11-15-11, S6 = 31-11-11, S7 = 24-8-16, and S8 = 19-6-12 in pot experiment at Dryland Agriculture Institute, West Texas A&;M University, Canyon, Texas, USA during winter 2009-10. A considerable variation in DM partitioning into various plant parts was observed in the four crop species at different growth stages and NPK source. At 30 DAE, 27% of the total DM per plant (TDMPP) was partitioned into roots and 73% into shoots (19% stems + 54% leaf). Only16 % of the TDMPP was partitioned into roots and 84% into shoots (18 % stem + 66 % leaf) at 60 DAE. At 90 DAE, 29% of TDMPP was partitioned into roots and 71 % into shoots (33 % stems + 38 % leaf) at 90 DAE. Percent DM partitioning into stems ranked first (33%) at 90 DAE > at 30 DAE (19%) > at 60 DAE (18 %). With advancement in crops age, DM partitioning into various crop parts increased. The root DM plant?1 (RDMPP) increased from 11.5–722 mg plant?1; stem DM plant?1 (STDMPP) from 8.3–889.0 mg plant?1; leaf DM plant?1 (LDMPP) from 23.1–1031.0 mg plant?1; shoot DM plant?1 (SHDMPP) from 31.3–1921 mg plant?1, and TDMPP increased from 42.9–2693.0 mg plant?1 at 30 and 90 DAE, respectively. Because of the higher N contents in S7 (24:8:16) and S6 (31:11:11) reduced the DM partitioning into various plants parts as well as TDMPP at all three growth stages. The adverse effects of S6 and S7 on DM partitioning was more on oats > rye > wheat > barley. The S4 with 10:10:10 (NPK) and :20S was not toxic at 30 DAE, but at 60 and 90 DAE it became toxic that adversely affected the DM partitioning as well as TDMPP probably may be due its high sulfur (20%) content which lacking in other NPK sources. The DM partitioning to various parts of barley and wheat was more than oats and rye at different growth stages (barley > wheat > rye > oats). Since the DM portioning values were determined on the average of five plants in pot experiment under organic soil at field capacity; in case of field experiments more research is needed on various crop species/varieties under different environmental conditions particularly under moisture stress condition.  相似文献   

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