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1.
用不同的时间和温度培养鸡致病性Ｅ．ｃｌｏｉ，４１℃，４８ｈ和３７℃，７２ｈ均可见众多的菌毛，而在４１℃，４８ｈ的条件下，活菌数及菌毛产量均高于３７℃，７２ｈ的。用提取的菌毛、超声波灭活的Ｅ．ｃｏｌｉ制成油乳苗免疫鸡，用同型Ｏ５０菌株经后胸气囊攻毒后，免疫鸡无死亡，而氢氧化铝胶菌苗免疫组的死亡率为２０％，未免疫攻毒组死亡率为３３．３％。各免疫组幸存鸡平均病变级数均显著低于未免疫攻毒组（Ｐ＜０．０１）。Ｅ．ｃｏｌｉ的菌毛剂量为１２０μｇ／只和８０μｇ／只的油乳苗免疫组，在免疫后用同型Ｏ５０菌株攻击时，保护指数最高；当剂量为６０μｇ／只时，保护指数较高；而用３０μｇ／只的剂量免疫时，免疫无效。雏鸡经含菌毛、不含菌毛的Ｅ．ｃｏｌｉ超声波灭活油乳苗和福尔马林灭活油乳苗免疫后用同型Ｏ５０菌株攻毒，含菌毛菌苗的保护指数均高于相应的不含菌毛菌苗的；超声波灭活菌体的油乳苗免疫组幸存鸡平均病变级数低于福尔马林灭活菌体的油乳苗免疫组的；各免疫组与未免疫攻毒组幸存鸡平均病变级数差异极显著（Ｐ＜０．０１）。 相似文献
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2009年5月,贵州省贵定县人工饲养的大鲵（娃娃鱼）发生了以四肢和腹侧的皮肤溃烂、口腔粘膜弥漫性出血以及肝脏肿大为临床病理特征的传染病。本研究对该传染病进行了病原分离、动物回归、菌株16S rRNA基因测序检测、药物敏感性、药物治疗及灭活乳化疫苗免疫保护方面的实验研究。试验结果表明,动物回归分离菌株与病原分离菌株其形态特征及理化特性一致,分离菌基因16S rRNA测序检测与嗜水气单胞菌的同源性达到99.57%以上,因此确诊该病为鱼类嗜水气单孢菌感染。致病株具有较强的耐药性,敏感药物治疗能抗菌,但皮肤溃烂组织、深部肌肉组织抗炎效果较差。经致病菌株灭活乳化免疫和免疫保护攻毒试验表明,灭活乳化疫苗免疫平均保护率达83.33%。 相似文献
3.
检测多杀性巴氏杆菌毒素抗体的单抗竞争ELISA方法的建立 总被引：1，自引：1，他引：0
本研究以纯化的多杀性巴氏杆菌毒素基因片段的原核表达产物作为抗原免疫小鼠制备单抗,并利用表达蛋白和多杀性巴氏杆菌毒素单抗酶结合物建立了竞争ELISA方法检测多杀性巴氏杆菌毒素抗体。经过研究确定抗原包被浓度为223ng／mL,待检血清最佳稀释度为1：2,酶标单抗工作浓度为1：3200,血清抑制率大于50%为阳性。应用单抗竞争ELISA和细胞毒性中和试验同时对82份血清进行猪多杀性巴氏杆菌毒素抗体检测,竞争ELISA的检出率为40.2％,细胞毒性中和试验检出率为36.6％,两者符合率达91.5％。试验结果表明,该ELISA方法特异性强,敏感性高,稳定性和重复性好,操作简便。本方法的建立在实验室诊断的标准化、猪群萎缩性鼻炎疫苗免疫效果的评价及流行病学调查方面具有应用价值。 相似文献
4.
进行了芒果叶水提物对部分畜禽常见致病菌的体外抑制试验以及对10日龄鸡胚人工感染鸡新城疫病毒(NDV)的抑制试验。抑菌试验结果表明,芒果叶水提物对大肠杆菌、多杀性巴氏杆菌、鸡白痢沙门氏菌、猪丹毒杆菌43-6混43-8、金色葡萄球菌86003、鸭疫里默氏杆菌、链球菌等致病菌有良好的抑菌效果。抑制NDV增殖试验结果表明,NDV强毒组在48h时的鸡胚保护率为10.00%,2.500g/mL芒果叶生药+NDV组的鸡胚保护率为63.64%,两者差异显著(P<0.05);1.250g/mL+NDV组、0.625g/mL+NDV组、0.313g/mL+NDV组的鸡胚保护率分别为72.72%、70.00%、69.23%,与强毒组相比,差异极显著(P<0.01),可见芒果叶水提物对NDV增殖具有抑制作用。 相似文献
5.
殷勤云 《广西农业生物科学》1982,(2)
以同位素钴—60γ射线处理,得到一株禽源的多杀性巴氏杆菌(Pasteurella multocida)链霉素耐药性(Str·R)突变株,暂称为GNCo 1株。 GNCo 1株以能在高浓度(500μg/m1)链霉素环境中生长和较亲代株生长慢为特征。毒力减弱,星布洛鸡以活菌102.9亿、广西三黄鸡以活菌60亿皮下注射不发病,家兔耐受30亿活菌皮下注射(更高未测);对小白鼠比亲代菌株PD_1毒力减弱10~(3-4)倍。与同时试验的“1010”株、“广农系”弱毒株毒力相近,比“731”株弱。通过有链霉素或无链霉素的人工培养基各30代,小白鼠18代,鸡3代(更多代未试),链霉素耐药性和毒力稳定,亦未发现链霉素依赖性突变。耐药性转移试验阴性。免疫不同品种、日龄的鸡13组,100％保护的5组(38.46％);80％保护1组(7.7％);60—67％保护4组(30.77％);50％保护2组(15.38％);20％保护1组(7.7％)。保护率50％以上的有12组,占13个试验组的92.3％。其中饮水免疫1组,保护4/6(67％)。两个月以后攻毒的组,亦保护80％以上。小群野外免疫不同品种的鸡1429只,无严重反应。GNCo1株在鸡体内主要停留于注射局部,存活期8—14天。 相似文献
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7.
传染性萎缩性鼻炎是养猪业中的一种重要的呼吸道传染病，产毒素多杀性巴氏杆菌是其重要的病原之一，外膜蛋白H（OmpH）在该菌的致病和免疫中发挥重要作用。本研究克隆了产毒素多杀性巴氏杆菌HN-13株的ompH基因，进行了序列分析，并在大肠杆菌中高效表达，用表达产物建立了间接ELISA方法。同时用生物信息学方法对ompH基因及其编码蛋白的结构特性进行了分析和预测，表明OmpH是一种通道蛋白。 相似文献
8.
制备兔多杀性巴氏杆菌(Pasteurella multocida,Pm)外膜蛋白A(OmpA)重组蛋白单抗,为兔多杀性巴氏杆菌病的诊断提供特异性强,灵敏度高的单克隆抗体。本研究选取并扩增Pm外膜蛋白基因OmpA,原核表达获得了的大小为37.6kD的OmpA重组蛋白,以纯化复性的兔多杀性巴氏杆菌OmpA重组蛋白作为免疫原,按常规方法免疫BALB/c小鼠(Mus musculus),取其脾细胞与SP2/0细胞融合,ELISA筛选出了4株分泌Pm OmpA蛋白单克隆抗体的杂交瘤细胞,分别命名为5D2、BC11、2A2和6A4。其中2A2细胞培养液效价为1∶256,5D2、BC11和6A4效价为1∶128;2A2腹水效价为1∶12800,其余3株达1∶6400。杂交瘤细胞经反复冻存、复苏及多次传代,仍能稳定分泌高效价抗体。Western blot显示,4株单克隆抗体均能与Pm OmpA重组蛋白重组发生特异性反应。用ELISA方法鉴定2A2单克隆抗体亚型为IgG2b,Protein A亲和纯化2A2腹水抗体,获得的单抗浓度为130μg/mL。选取纯化的2A2单克隆抗体进行潜在应用研究,Western blot与间接免疫荧光结果显示,单克隆抗体能与兔多杀性巴氏杆菌分离菌株发生特异性反应。表明利用体外重组表达兔多杀性巴氏杆菌OmpA融合蛋白制成的杂交瘤能够稳定分泌效价高、特异性强的单克隆抗体,可用于兔多杀性巴氏杆菌诊断,本研究结果为兔多杀性巴氏杆菌诊断试剂盒的研制提供技术参数。 相似文献
9.
猪胸膜肺炎放线杆菌毒素Ⅱ基因的克隆、表达及其免疫原性 总被引：1，自引：2，他引：1
以本实验室分离的猪胸膜肺炎放线杆菌血清2型(Actinobacilluspleuropneumoniaeserotype2,APP-2)菌株HB08的基因组为模板,扩增了2871bp的APP毒素Ⅱ的结构基因apxIIA,并克隆到pET-28a原核表达载体中构建重组表达质粒pET28aIIA,转化到大肠杆菌(Escherichiacoli)BL21(DE3),经SDS-PAGE和Westernblot分析表明,表达的重组蛋白具有良好的反应活性。将表达的蛋白经或不经复性处理,与纯化的天然毒素Ⅱ分别免疫昆明小鼠,同时设PBS空白对照组,每组12只,间隔2周免疫2次,采血检测其抗体效价,二免后2周用致死剂量的APP血清7型(APP-7)菌株(1.08×108CFU(菌落形成单位,colonyformunit)/只)腹腔攻击。结果显示,复性蛋白组的保护率为83.3%,非复性蛋白组的保护率为58.3%,天然毒素蛋白对照组保护率为91.7%,空白对照组小鼠全部死亡,说明复性的重组毒素Ⅱ具有良好的免疫原性。 相似文献
10.
鸡传染性鼻炎自家油乳苗免疫的研究 总被引：1，自引：0，他引：1
本试验用从广西分离的二株引起鸡传染性鼻炎的副鸡嗜血杆菌 ( H aemophilus paragallinarum,H.pg) GX 97、GX 981株研制成自家油乳苗 ,进行后备鸡免疫试验 ,免疫后 3、 6、 9和 1 2个月进行免疫抗体检测和攻毒试验。结果表明 ,免疫后 6个月 90 %免疫鸡的抗体滴度在 1∶ 640以上 ,9个月抽样攻毒有 75%～ 80 %的鸡获得保护。大田免疫试验也获得了良好的效果。证明自家油乳苗免疫接种也是防制该病的一个有效途径。 相似文献
11.
Varieties of market cheese were analyzed for alkaline phosphatase by the modified rapid colorimetric method of the American Public Health Association (APHA) and the official AOAC method, 16.304-16.306. In the APHA method, 5 g cheese (pH less than 7.0) is macerated with 2 mL 1:1 carbonate buffer, or 2 mL water (for cheese with pH greater than 7.0). Addition of 0.1 mL magnesium acetate (1 mg magnesium) to test portions of cheese extracts yielded reproducible and quantitative recovery of added phosphatase. In the AOAC method, macerating 0.5 g cheese with 1 mL borate buffer before adding milk phosphatase improved recovery among cheeses. Addition of magnesium ion increased phosphatase activity in some cheeses. Phosphatases in blue mold-ripened and Swiss cheeses were inactivated by heat faster than was milk phosphatase, yet milk phosphatase added to various soft cheeses was completely inactivated at 60 degrees C for 10 min. The lability of phosphatase was due to the heat-denaturing effect of NaCl present in finished cheeses. Some Mexican style soft cheeses contained both heat-labile and heat-stable phosphatases. These data suggest that the phosphatase test to differentiate milk and microbial phosphatases on the basis of repasteurization and analysis of cheese is no longer valid. 相似文献
12.
利用二氧化氯(ClO2)对水、苹果汁中和苹果表面的酿酒酵母1450(Saccharomyces cerevisiae)进行杀菌处理。结果表明：6、12 mg/L的ClO2处理15 min使水中酵母分别减少(5.81±0.12)和(6.20±0.05)lg cfu/mL;100 mg/L的ClO2对苹果汁中酵母的杀菌作用不明显,200 mg/L的ClO2处理1 h可将果汁中(3.79±0.04)lg cfu/mL的酵母完全杀死,使果汁中(6.48±0.03)lg cfu/mL的酵母减少(3.67±0.05)lg cfu/mL,300 mg/L的ClO2可将苹果汁中(6.52±0.06)lg cfu/mL的酵母完全杀死;10、20、30和40 mg/L的ClO2处理1 h使苹果表面接种量为(4.95±0.02)lg cfu/mL的酵母数分别减少(3.41±0.02)、(3.64±0.08)、(3.80±0.04)、(4.47±0.09)lg cfu/mL,50 mg/L的ClO2处理1 h,可将接种于苹果表面的酵母全部杀死,接种量进一步增加,ClO2对苹果表面酵母的杀灭效果将会减弱;ClO2处理对苹果汁的理化指标影响较小。 相似文献
13.
Glycoprotein derived from the hot water extract of mint plant, Perilla frutescens britton 总被引：1，自引：0，他引：1
Asada M Fukumori Y Inoue M Nakagomi K Sugie M Fujita Y Tomizuka N Yamazaki Y Oka S 《Journal of agricultural and food chemistry》1999,47(2):468-472
Glycoprotein showing inhibitory activity against mast cell degranulation and hyaluronidase activity was purified from the hot water extract of mint plant (Perilla frutescens Britton). The purified inhibitor gave a single band detected with Coomassie brilliant blue staining and periodic acid-Schiff staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The molecular mass was estimated to be 6.0 kDa on SDS-PAGE. The inhibitor did not become inactivated when boiled for 30 min or digested with trypsin, V8 protease, or proteinase K but was inactivated by NaIO(4) oxidation. The inhibitor prevented mast cell degranulation and hyaluronidase activity (IC(50) = 0.42 mg/mL) in a dose-dependent manner. The inhibitor also inhibited the protein kinase C activity. It is possible to purify and characterize a glycoprotein with putative pharmacological properties from mint plants. 相似文献
14.
根据GenBank发表的鸭IL-18 cDNA基因序列，设计、合成一对引物，直接从成年麻鸭脾淋巴细胞提取总RNA，经反转录-聚合酶链反应（RT-PCR）扩增麻鸭IL-18成熟蛋白基因，扩增产物进行了T-A克隆、测序，获得了麻鸭IL-18成熟蛋白基因全序列，其大小为513 bp，编码一条由170个氨基酸残基组成的多肽。将该基因片段亚克隆到原核表达载体pQE30，构建重组表达质粒pQE30-mDuIL18，转化大肠杆菌M15，并用IPTG 诱导。重组菌菌体裂解物经SDS-PAGE可检测到相对分子质量为19.76 Ku的重组蛋白，Western blotting证实该重组蛋白可与兔抗鸡IL-18血清发生特异性反应。表达产物以包涵体形式存在，包涵体经变性、复性处理后进行鸭T淋巴细胞转化试验表明，重组蛋白具有明显促进鸭T细胞转化的生物学活性。用mDuIL-18（150 ng or 200 ng /鸭）和AIV 疫苗免疫鸭2周后，HI抗体平均滴度达到7.5–7.7 log 2 ，而只用AIV 疫苗免疫和用mDuIL-18（100 ng /鸭）和AIV 疫苗免疫的HI抗体平均滴度仅达到6.3–6.6 log 2，表明mDuIL-18提高AIV灭活油乳苗诱导的免疫应答。这为研究鸭IL-18结构和生物学应用，以及研制新型免疫佐剂和免疫调节剂奠定基础。 相似文献
15.
猪胚胎干细胞培养、分离和传代 总被引：4，自引：0，他引：4
摘要:利用五指山小型猪近交系不同发育阶段的早期胚胎为材料，探索猪胚胎干细胞(embryonic stem cells,ES)培养、分离、传代的影响因素，以选择猪胚胎干细胞建系的适宜条件。收集五指山猪第5～10 d胚胎(配种当天记为0 d)，以 STO细胞[STO细胞是来自SIM小鼠(S)胚胎对硫代鸟嘌呤(thioguanine,T)和乌本苷(ouabain,O)有抗性的成纤维细胞系]为饲养层，分别采用两种培养液: 杜氏培养液(Dulbecco's modified eagle medium ，DMEM) + 10%胎牛血清（fetal bovine serum，FBS）+ 10 %新生牛血清（newborn bovine serum，NBS）+1 000 IU/mL白血病抑制因子（leukemia inhibitory factor，LIF）+ 30 ng/mL干细胞生长因子（stem cell growth factor , SCF）+20 ng/mL碱性成纤维细胞生长因子(basic fibroblast growth factor ，bFGF)或DMEM + 10% NBS + 10% FBS + 1 000 IU/mL LIF + 30 ng/mL SCF。比较发现，培养液中是否加入bFGF对胚胎干细胞的培养无显著影响；实验共收集胚胎124枚，其中D5～6胚胎18枚，胚胎贴壁率为68.8% ，内细胞团出现率为6.3% ，未能传代；收集D7孵化囊胚27枚，贴壁率为100% ，内细胞团出现率为38.9% ，传代1次失败；D9胚胎18枚，贴壁率为100%，传代后ES细胞生长较缓慢；收集D10胚胎52枚，胚胎贴壁率为100%，传代率为100%，传代后可出现ES细胞克隆点；实验过程中比较了胚径对干细胞培养的影响，发现胚径越大，培养效果越好，胚径大于100μm,分离ICM后出现干细胞集落成功率达100%，并得到了AP染色呈阳性的传4代的ES细胞集落。 相似文献
16.
Extraction protocols for β‐glucan from oat flour were tested to determine optimal conditions for β‐glucan quality testing, which included extractability and molecular weight. We found mass yields of β‐glucan were constant at all temperatures, pH values, and flour‐to‐water ratios, as long as sufficient time and enough repeat extractions were performed and no hydrolytic enzymes were present. Extracts contained about 30–60% β‐glucan, with lower proportions associated with higher extraction temperatures in which more starch and protein were extracted. All commercial starch hydrolytic enzymes tested, even those that are considered homogenous, degraded β‐glucan apparent molecular weight as evaluated by size‐exclusion chromatography. Higher concentration β‐glucan solutions could be prepared by controlling the flour‐to‐water ratio in extractions. Eight grams of flour per 50 mL of water generated the highest native β‐glucan concentrations. Routine extractions contained 2 g of enzyme‐inactivated flour in 50 mL of water with 5mM sodium azide (as an antimicrobial), which were stirred overnight, centrifuged, and the supernatant boiled for 10 min. The polymer extracted had a molecular weight of about 2 million and was stable at room temperature for at least a month. 相似文献
17.
嗜水气单胞菌外膜蛋白(OMP)在乳酸乳球菌中的表达及其对BALB/c小鼠的免疫保护效果 总被引：1，自引：0，他引：1
乳酸球菌(Lactococcus lactis)是一种安全级微生物,为活载体疫苗传递抗原的理想选择.嗜水气单胞菌(A eromonas hydrophila,Ah)能引起鱼类等多种动物的败血病,因其血清型众多,寻找共同保护性抗原是进行疫苗研究的重点.为探究嗜水气单胞菌AS1.927株外膜蛋白(OMP)在乳酸乳球菌中的表达和检测其表达产物对小鼠的免疫保护效果,本研究将己克隆的ompA基因经BamH Ⅰ和Xba Ⅰ双酶切后连接载体pNZ8048,转化乳酸乳球菌NZ9000,nisin诱导表达,并进行SDS-PAGE分析鉴定.结果显示,重组基因工程菌L.lactis[pNZ-ompA]表达的融合蛋白分子量约为36.2 kD,成熟蛋白分子量约为33.7 kD.将重组基因工程菌L.lac tis [pNZ-ompA]口服免疫BALB/c小鼠(Mus musculus),用放射免疫法检测末次免疫一周后的各组小鼠肠粘膜分泌型免疫球蛋白A(sIgA)的水平以及用间接ELISA法检测小鼠血清特异性抗体IgG,结果发现,该重组基因工程菌能显著提高sIgA的分泌(P＜0.05);同时在小鼠血清中的特异性抗体含量也显著高于对照组(P＜0.05).末次免疫两周后用100 LD50的Ah AS 1.927(3.3×105 cfu/mL)腹腔注射攻击小鼠,口服重组菌对小鼠的相对免疫保护力(relative percent survival,RPS)为87.5％,表明重组基因工程菌可诱导小鼠对AhAS1.927产生一定的免疫保护作用.该结果将为开发安全有效的口服基因工程鱼用疫苗提供一定的实验基础. 相似文献
18.
Li Y Song C Zhang K Wang M Yang K Yang A Jin B 《Journal of agricultural and food chemistry》2008,56(2):337-342
A highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) kit was established for quantifying ovomucoid from hen's egg white, which has been considered as one of the major allergen in egg white. The detection limit reached 0.041 ng/mL, and linearity ranged from 0.1 to 6.25 ng/mL. Intra- and interassay coefficient variations were all lower than 5% at three concentrations (0.5, 2.5, and 5 ng/mL). No cross-reactivity was observed with bovine serum, horse serum, goat serum, human serum, duck egg white, goose egg white, quail egg white, and pigeon egg white, but a low level of cross-reactivity was found with chicken serum. The ELISA kit was established on the basis of two monoclonal antibodies (mAbs) recognizing different epitopes of ovomucoid. However, these mAbs were generated using commercially purified ovalbumin as immunogen. Studies on the relative allergenicity and antigenicity of egg white protein have been performed by many researchers, but there were controversial opinions reported previously because of the impurity of each egg white protein used in various studies. In the present work we measured the degree of ovomucoid contamination in commercially purified ovalbumin sample, and the value was about 11%. We also determined the ovomucoid residue in influenza vaccine samples for the first time. These data showed that the ELISA kit we established could serve as an effective method for precisely quantifying concentrations of ovomucoid in the egg industry and as a useful tool for the research of allergenicity and antigenicity of hen's egg proteins. 相似文献
19.
摘要：纯化的重组子pM-VP2-E290阳性质粒，与Bac-N-Blue DNA共转染昆虫细胞sf9，通过同源重组，形成具有感染活性的重组杆状病毒，并利用重组病毒的LacZ表型进行噬斑纯化。纯化后的高效价重组杆状病毒在昆虫细胞中进行表达，表达产物应用SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）、免疫印迹（Western-blot）和免疫酶斑点技术（Dot-ELISA）进行分析，确定所表达的蛋白分子量大小约为67KD，且具有天然蛋白的抗原特异性。应用免疫电镜对表达产物进行观察，可见到细小病毒样颗粒。病毒样颗粒在无免疫佐剂参与的情况下，免疫6-8周龄的BALB/c小鼠，同时设PPV的灭活疫苗免疫组及PBS接种组作为参比对照，检测小鼠的细胞免疫和体液免疫学指标。结果表明，表达蛋白所形成的细小病毒样颗粒，不仅能诱导小鼠机体产生抗CSFV的特异性CTL反应，还能刺激小鼠产生高效价的抗PPV的特异性抗体。而且机体所产生的抗体效价显著高于灭活疫苗对照组。 相似文献
20.
Biochemical and functional properties of Atlantic salmon (Salmo salar) muscle proteins hydrolyzed with various alkaline proteases 总被引：6，自引：0，他引：6
Protein hydrolysates (5, 10, and 15% degrees of hydrolysis) were made from minced salmon muscle treated with one of four alkaline proteases (Alcalase 2.4L, Flavourzyme 1000L, Corolase PN-L, and Corolase 7089) or endogenous digestive proteases. Reaction conditions were controlled at pH 7.5, 40 degrees C, and 7.5% protein content, and enzymes were added on the basis of standardized activity units (Azocoll units). Proteases were heat inactivated, insoluble and unhydrolyzed material was centrifuged out, and soluble protein fractions were recovered and lyophilized. Substrate specificities for the proteases was clearly different. Protein content for the hydrolysates ranged from 71.7 to 88.4%, and lipid content was very low. Nitrogen recovery ranged from 40.6 to 79.9%. The nitrogen solubility index was comparable to that of egg albumin and ranged from 92.4 to 99.7%. Solubility was high over a wide range of pH. The water-holding capacity of fish protein hydrolysates added at 1.5% in a model food system of frozen minced salmon patties was tested. Drip loss was on average lower for the fish protein hydrolysates than for egg albumin and soy protein concentrate, especially for Alcalase hydrolysates. Emulsification capacity for fish protein hydrolysates ranged quite a bit (75-299 mL of oil emulsified per 200 mg of protein), and some were better than soy protein concentrate (180 mL of oil emulsified per 200 mg of protein), but egg albumin had the highest emulsifying capacity (417 mL of oil emulsified per 200 mg of protein). Emulsification stability for fish protein hydrolysates (50-70%) was similar to or lower than those of egg albumin (73%) or soy protein concentrate (68%). Fat absorption was greater for 5 and 10% degrees of hydrolysis fish protein hydrolysates (3.22-5.90 mL of oil/g of protein) than for 15% hydrolysates, and all had greater fat absorption than egg albumin (2. 36 mL of oil/g of protein) or soy protein concentrate (2.90 mL of oil/g of protein). 相似文献