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1.
苹果属种、杂交种及品种之间关系的AFLP分析 总被引：3，自引：1，他引：2
用AFLP技术,对72个苹果属植物样本(34个种、变种、杂交种及38个品种)进行遗传多样性分析.在64对AFLP引物中选出6对引物进行扩增,得到有效谱带1692条,其中多态谱带1547条,多态谱带比率为91.4%.对72个样品的试验数据进行聚类分析,它们的相似系数为0.54～0.82.在相似系数0.60处参试材料被分为4大类,即佛罗伦萨苹果(Malus florentina)为一类;绿苹果组(Sect.Choromeles)和多胜苹果组(Sect.Docyniopsis)分别各聚一类;花楸组(Sect.Sorbomalus)、脱萼组(Sect.Gymnomeles)和苹果组(Sect.Malus)聚为一类,所有苹果品种和观赏海棠品种全聚在原产亚洲的花楸组、脱萼组及苹果组中.亚洲原产的苹果属植物对于苹果及观赏海棠品种的发展起重要作用.根据试验结果及文献资料,支持将佛罗伦萨苹果组(Sect.Florentinae Cheng M.H.)成立单种组;建议将原属花楸组植物除佛罗伦萨苹果外,全部并入苹果组;取消脱萼组,将其组内的植物全部并入苹果组. 相似文献
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Jianming Gao Yong Zhang Chunguo Wang Shougong Zhang Liwang Qi Wenqin Song 《New Forests》2009,37(3):333-344
Amplified fragment length polymorphism (AFLP) was used in genetic fingerprinting of 22 elite Chinese Populus deltoides and Populus × canadensis accessions. The results indicated that each of the nine AFLP primer pairs selected generated fingerprint profiles that were
unique to each of the accessions. Therefore, each accession was definitively identified by any of the nine primer pairs. Furthermore,
the inter-accession genetic relationships inferred based on 461 polymorphic fragments from the nine AFLP primer pairs were
largely consistent with phylogenetic relationships based on morphologic traits. Bootstrap analysis showed that three AFLP
primer pairs were required to obtain genetic similarity values with a maximum CV of 10% while 10 AFLP primer pairs could give
a maximum CV of 5%. Thus, AFLP can readily be applied for a rapid and accurate evaluation of the degree of similarity between
poplar cultivars. In this study, the number of AFLP fragments used was sufficient to establish a reliable estimate of genetic
similarity among accessions, with a maximum CV of 5.12%. Therefore, the information on the genetic relationships among the
poplar accessions generated in this study in connection with knowledge on agronomic traits may have an impact on poplar breeding
and planting in China. 相似文献
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分子标记法和形态学特征对杏仁栽培种及野生种基因型的遗传关系构建的辨别 总被引：1，自引：0，他引：1
Karim Sorkheh Behrouz Shiran Soghra Kiani Nazanin Amirbakhtiar Sadegh Mousavi Vahid Rouhi Shahram Mohammady-D Thomas M.Gradziel Lyudmyla V.Malysheva-Otto Pedro Martínez-Gómez 《林业研究》2009,20(3):183-194
应用23个形态学特征,19个扩增片段长度多态性(AFLP)引物组合,80个随机扩增多态DNA(RAPD)引物和32个简单序列重复(SSR)引物对,比较三种分子标记法在29个杏仁栽培种和3个野生种遗传关系构建中的信息景和效率.根据预期杂合度的评价,与AFLPs和RAPDs相比,SSRs具有较高水平的多态性和较大的信息量.AFLPs预期朵合度值最低,但其辨别效率值最高,因为AFLPs能揭示每个反应中的大量条带,导致各种类型的多样性指数均较高.三种分子标记法对杏仁基因型的辨别效率均较高,只是SSRs无法辨别‘Monagha'和‘Sefied'杏仁基因型.三种分子标记法基因型相似性相关系数统计上显著,但SSR数据要低于RAPDs和AFLPs的值.尽管三种分子标记法树形图拓扑结构存在一些差异,但相似性水平均较高.SSRs、RAPDs和AFLPs的系统树图及其综合数据都能依据地理散布反映大多数栽培种的关系.AMOVA检测到每个地理组中栽培种和野生种的变异.辅助程序分析表明,实验所应用的标记物数量足以保证基因相似性估计的可靠性和标记法间的比较是有意义的. 相似文献
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Genetic relationships among Prunus mume var. pendula were studied by using AFLP markers. 18 accessions representing 14 cultivars ofPrunus murne var. pendula were selected from the germplasm collection at the Research Center of China Mci Flower. Seven Mse I-EcoR I AFLP primer combinations revealed 450 legible bands, and 269 of which were polymorphic markers. A similarity matrix was prepared using the simple matching coefficient of similarity and Nei‘s (72) distance coefficient. A UPGMA dendrogram demonstrated the genetic relationships of the cultivars. The information given by AFLP markers was basically consistent with the morphological classification and the evolutionary history of the morphotypes, and roughly supported the new revised classification system for Chinese Mci Cultivars. But there were still several exceptions: 1) the ‘Guhong Chuizhi‘ inserted between the ‘Tiaoxue Chuizhi‘ and the ‘Danfen Chuizhi‘; 2) the ‘Wufu Chuizhi‘ kept off the Pink Pendant Form, and the ‘Moshan Chuizhi‘ was removed from Viridiflora Pendant Form; 3) the ‘Danbi Chuizhi‘ and the ‘Shuangbi Chuizhi‘ of Viridiflora Pendant Form got together well but fell within the Pink Pendant Form. 相似文献
6.
引进葡萄柚品种基因资源的AFLP分析 总被引：3，自引：0，他引：3
采用AFLP分子标记技术,对引进的9个葡萄柚品种基因资源进行分析,评价引进资源的遗传多样性,揭示遗传结构和品种间亲缘关系,为引进葡萄柚品种的进一步开发利用和遗传改良提供基础信息。结果表明,筛选出的7对引物共扩增出263条带,多态带比率为7.22%。多态性标记百分率、观测等位基因数、有效等位基因数、Nei’s基因多样性指数和Shannon信息指数在物种水平分别为7.22%、0.023 4、0.008 0、0.6592和0.258 5,而在品种水平它们分别为2.41%、1.024 1、1.013 2、0.008 0和0.012 2。基因分化系数Gst为0.659,表明葡萄柚的遗传差异主要来自于品种之间。9个品种间的遗传距离变幅在0.000 5~0.037 1之间,平均为0.025 8。基于UPGMA的聚类结果将9个品种分为2组,火焰、矮化、亨路比、哈路比、帕利斯和瑞路比聚为一组,里德马叙、里约红和星路比构成另一组。 相似文献
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为给锥栗杂交育种和遗传作图的亲本选配提供参考,应用SSR分子标记技术,分析了福建省建瓯市17个锥栗主栽农家品种的遗传多样性。结果表明:不同农家品种遗传多样性丰富,利用筛选出的12对引物组合扩增的片段大小主要在50~2 000 bp之间,共扩增出180个位点,多态位点163个,占90.56%;17个锥栗主栽农家品种的遗传相似性系数在0.509 2~0822 1之间,乌壳长芒和牛角仔(晚熟)的相似性系数最大,中尖嘴与白露仔的相似性系数最小,且在相似性系数为0.60处,大尖嘴和中尖嘴单独聚为I类,在相似性系数为0.77处,第II类又被分为A~H共8个组;SSR引物组合Cs CAT33和Cm TCR4共同构建了17个锥栗主栽农家品种的DNA指纹图谱。 相似文献
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本研究在对选择性扩增引物进行筛选的基础上,对银白杨、N001杨及银白杨×N001杨杂交子代共10个样本进行了AFLP试验,并利用Popgene和NTsys软件分别对遗传多样性参数及个体聚类进行分析。结果表明:筛选出的8对选择性扩增引物,共扩增出969条谱带,多态性条带数929条,多态性比例为95.76%,平均有效等位基因数为1.4329,Nei's平均遗传多样性指数为0.2662,Shannon平均信息指数为0.4161。不同杂交子代在E-ACC/M-CAC引物对上均有特异位点,这些特异位点用于申报优良品种保护。在通过NTsys软件聚类后,在遗传相似系数为0.76时,将10个试样分为2个大支,其中N001杨为1个AFLP群,银白杨及8个杂交子代为1个AFLP群;当遗传相似系数为0.79时,银白杨与8个杂交子代的AFLP群又细化为2个亚群,其中杂交子代2和杂交子代6聚合为亚群1,银白杨和其余6个杂交子代为亚群2。本研究的结果多态性比例很高,对样品的区分率达到100%,为杨树AFLP分析检测提供了较好的应用和指导借鉴价值。 相似文献
11.
Jianming Gao Shougong Zhang Liwang Qi Yong Zhang Chunguo Wang Ruiyang Chen Wenqin Song 《Frontiers of Forestry in China》2007,2(2):204-209
Accurate identification of varieties (clones) and knowledge of their genetic relationships are essential for poplar breeding
and variety management. In this study, 21 elite poplar varieties of Tacamahaca and Aigeiros in China were fingerprinted using amplified fragment length polymorphism (AFLP) markers. Four AFLP primer pairs developed
generated totally 181 AFLP polymorphic fragments, and in particular, each primer pair generated fingerprint profiles specific
to each of the tested varieties. The genetic relationships among the varieties were evaluated by dendrograms and multidimensional
scaling (MDS). The results showed that the tested poplar can be classified into five groups, and indicated the clear separation
of varieties of different sections of poplar and the primary distinction between native and exotic poplar varieties. This
study indicated that tested poplar varieties could be identified by their fingerprint profiles and that genetic relationships
deduced from the study were consistent with their genealogy. In addition, our results demonstrated that AFLP could be used
to construct DNA fingerprints of polar clones at a large-scale level and to determine genetic relationships of poplar varieties.
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Translated from Acta Botanica Yunnanica, 2006, 28(1): 85–90 [译自: 云南植物研究] 相似文献
12.
腰果混杂群体的遗传组成分析 总被引：2，自引：0，他引：2
利用AFLP分子标记技术对54个单株构成的腰果混杂群体的遗传组成进行分析,评估其遗传变异水平,旨在为合理有效利用该资源提供科学理论依据。结果显示,筛选出的8对引物组合共扩增出467条谱带,其中,多态性带为179条。多态性带百分率、观测等位基因数、有效等位基因数、Nei’s基因多样性指数和Shannon信息指数分别为38.33%、1.383 3、1.150 4、0.094 6和0.149 2。遗传距离的变异范围在0.017 1~0.250 5之间,平均为0.103 5。UPGMA聚类结果将54个样株归于4个复合组和2个独立组。 相似文献
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十一种槭属植物遗传多样性AFLP分析 总被引：4，自引：0，他引：4
采用了AFLP技术分析了槭属十一种植物的遗传多样性,从12对AFLP引物中筛选出5对多态性高、分辨率清晰的引物,其中选择最优的3个引物组合分别扩增出101、163、178条多态性带。三对引物(E ACG/M CAG、E ACA/M CAT、E AAC/M CAC)共扩增出484条带。分析结果按UPGMA类平均法进行聚类,聚类结果显示将11种械属植物聚为I、Ⅱ、Ⅲ三大类,其中羊角槭和栓皮槭,长尾秀丽槭和毛脉槭,复叶槭与剑叶槭之间的亲缘关系最近。 相似文献
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舞毒蛾不同地理种群基于AFLP分子标记的遗传分析 总被引：1，自引：0，他引：1
舞毒蛾是一种食性很广、危害很大的世界性林木害虫,根据其地理分布和生活特性,现在被分为亚洲型和欧洲型2种。对来自俄罗斯远东地区、蒙古、日本、美国和中国5个地理种群,共26份舞毒蛾样品进行AFLP分子标记研究。成功建立并优化舞毒蛾AFLP分析体系,从16对引物组合中筛选出3对扩增条带多、多态检出率高的荧光标记引物组合,利用CEQ-8000遗传分析仪进行毛细管电泳及数据分析,共检测到507个多态性位点。通过PAUP软件对AFLP数据进行UPGM和NJ树的聚类分析以及遗传距离分析,结果表明:5个地理种群舞毒蛾明显分成欧洲型(美国种群)和亚洲型,其中亚洲型又可分成俄罗斯、日本、中国及蒙古3个类群。美国种群间遗传变异比其他种群较大,中国种群与美国种群遗传距离最大,而与蒙古种群遗传分化最小。从分子水平上研究舞毒蛾不同种群的遗传分类情况,揭示利用AFLP分子标记技术可以区分舞毒蛾不同地理种群的基因型,为研究舞毒蛾的起源、入侵与扩散、遗传与变异以及检疫措施的制定等方面提供科学依据。 相似文献
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In light of the economic importance of buckwheat as well as existence of enormous accessions of Fagopyrum species in the Himalayan regions of India, the characterization of tartary buckwheat for rutin content variation vis-à-vis DNA fingerprinting was undertaken so as to identify fingerprint profiles unique to high rutin content accessions. Rutin content analysis in mature seeds of 195 accessions of Fagopyrum tataricum showed a wide range of variation (6 μg/mg to 30 μg/mg D.W.) with most of the accessions (81%) containing 10-16 μg/mg of rutin followed by 14% accessions with significantly higher rutin content (17 μg/mg to 30 μg/mg) and 5% accessions with low rutin content (≤10 μg/mg). AFLP fingerprinting of 18 accessions having high (≥17 μg/mg) and low rutin content (≤10 μg/mg) with 19 EcoRI/MseI primer combinations yielded 136 polymorphic fragments out of total 907. The hierarchical and model-based cluster analyses of AFLP data strongly suggested that the 18 populations of F. tataricum were clustered into two separate groups. The high and low rutin content accessions were clustered into two separate groups based on AFLP fingerprinting. The AFLP fingerprints associated with high rutin content accessions of F. tataricum are expected to be useful for evaluation, conservation and genetic improvement of buckwheat. 相似文献
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为了探讨银白杨、马氏杨及银白杨×马氏杨杂交子代间的遗传多样性参数及个体聚类关系,利用8对引物对银白杨、马氏杨及银白杨×马氏杨杂交子代4个样本进行了AFLP分析。结果表明,8对引物共扩增出780条扩增谱带,其中多态性条带数为715条,多态性比例为91.65%,平均有效等位基因数为1.5017,Nei’s平均遗传多样性指数为0.3065,Shannon平均信息指数为0.4679。不同杂交子代在E-AAC/M-CAG引物对上均有特异性位点,这些特异位点可用于品种鉴定。聚类研究发现,在相似系数为0.76时,试验样品分成2个AFLP群,其中马氏杨为1个AFLP群,银白杨及2个杂交子代为1个AFLP群;当相似系数为0.78时,银白杨和2个杂交子代的AFLP群又划分为2个亚群,其中杂交子代1和杂交子代2聚合为亚群1,银白杨为亚群2。本研究的结果多态性比例很高,对样品的区分率达到100%,为杨树AFLP分析检测提供了指导借鉴价值。 相似文献
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枣优良品种分子鉴别系统的开发 总被引：2，自引：0，他引：2
利用荧光AFLP分子标记技术对26个枣优良品种及1个酸枣品种进行分子鉴别.8对引物共扩增得到886条带,其中特征性条带(某品种特有)112条,缺失条带(某品种缺少)60条,利用这些特殊性条带可有效地对供试材料进行鉴别.从3对引物中选择21条多态性丰富的DNA条带,将其转换成1、0数据,在此基础上开发出了用于枣优良品种鉴别的计算机应用软件. 相似文献
20.
Lin J Gunter LE Harding SA Kopp RF McCord RP Tsai CJ Tuskan GA Smart LB 《Tree physiology》2007,27(11):1575-1583
Salix matsudana Koidz. cultivar 'Tortuosa' (corkscrew willow) is characterized by extensive stem bending and curling of leaves. To investigate the genetic basis of this trait, controlled crosses were made between a corkscrew female (S. matsudana 'Tortuosa') and a straight-stemmed, wild-type male (Salix alba L. Clone 99010). Seventy-seven seedlings from this family (ID 99270) were grown in the field for phenotypic observation. Among the progeny, 39 had straight stems and leaves and 38 had bent stems and curled leaves, suggesting that a dominant allele at a single locus controls this phenotype. As a first step in characterizing the locus, we searched for amplified fragment length polymorphism (AFLP) and randomly amplified polymorphic DNA (RAPD) markers linked to the tortuosa allele using bulked segregant analysis. Samples of DNA from 10 corkscrew individuals were combined to produce a corkscrew pool, and DNA from 10 straight progeny was combined to make a wild-type pool. Sixty-four AFLP primer combinations and 640 RAPD primers were screened to identify marker bands amplified from the corkscrew parent and progeny pool, but not from the wild-type parent or progeny pool. An AFLP marker and a RAPD marker linked to and flanking the tortuosa locus were placed on a preliminary linkage map constructed based on segregation among the 77 progeny. Sectioning and analysis of shoot tips revealed that the corkscrew phenotype is associated with vascular cell collapse, smaller cell size in regions near the cambium and less developed phloem fibers than in wild-type progeny. Identification of a gene associated with this trait could lead to greater understanding of the control of normal stem development in woody plants. 相似文献