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1.
In August 1990, a 2-ha plantation was established in an area where rainfall (about 515 mm year(-1)) was insufficient to meet evaporative demand. On nine occasions between September 1991 and April 1993, pressure-volume curves were constructed for irrigated and rainfed Eucalyptus globulus ssp. globulus Labill. and E. nitens (Deane and Maiden) Maiden trees. During the experiment, rainfed trees experienced six periods when predawn water potential was significantly lower than that of irrigated trees. In early spring of 1991 and 1992, osmotic potentials at full turgor and turgor loss point in the irrigated E. nitens were significantly lower than at other times of the year, probably because of winter hardening. Water stress reduced osmotic potential and increased bulk elastic modulus in E. nitens, whereas the reverse occurred in E. globulus. However, treatment differences with respect to changes in osmotic and elastic properties were commonly overshadowed by interspecific differences. These were most apparent at the end of the sixth period of water stress when osmotic potentials at full and zero turgor were significantly higher and bulk elastic modulus and relative water content at turgor loss point were significantly lower in E. globulus than in E. nitens. We conclude that the drought-tolerance responses of E. globulus make it a more suitable species than E. nitens for establishment on sites where moderate water stress is experienced. 相似文献
2.
Leaf water relations and stomatal behavior of four allopatric Eucalyptus species planted in Mediterranean southwestern Australia 总被引：2，自引：0，他引：2
In 1986, four allopatric Eucalyptus species (E. camaldulensis Dehnh, E. saligna Smith, E. leucoxylon F. Muell and E. platypus Hook.) were planted together in a 480-mm rainfall zone, in 8-m wide contour belts as part of a plan to minimize waterlogging and secondary salinization. Throughout 1997, 1998 and 1999, there was significant inter-specific variation in predawn leaf water potential (Psi(pd)); however, maximum stomatal conductance (g(sm)) only differed significantly between species in mid to late summer. Relationships between g(sm) and Psi(pd) were significant and showed that stomata of E. camaldulensis were significantly more sensitive to Psi(pd), and presumably soil water potential, than stomata of E. leucoxylon or E. platypus. When applied to the Psi(pd) data, these relationships predicted that g(sm), and by inference transpiration, varied much less between species than Psi(pd). Diurnal measurements throughout the season confirmed this prediction, and showed that E. camaldulensis and E. saligna avoided drought by gaining access to deeper water, whereas E. leucoxylon and E. platypus maintained greater g(sm) at a given water stress than E. camaldulensis or E. saligna. Osmotic potentials measured after rehydration and water release curves of the leaves indicated that different mechanisms accounted for the apparent drought tolerance of E. leucoxylon and E. platypus. In summer, E. leucoxylon reduced osmotic potential at full and zero turgor by similar amounts compared with winter. In summer, E. platypus had a significantly lower bulk elastic modulus and relative water content at turgor loss point than E. camaldulensis, E. saligna or E. leucoxylon. This elastic adjustment resulted in a larger difference between osmotic potential at full and zero turgor in summer than in winter. The inherently low osmotic potential in E. leucoxylon and elastic adjustment in E. platypus resulted in turgor loss at a similar and significantly lower water potential than in E. camaldulensis or E. saligna. These results have implications for species selection for planting to manage groundwater recharge in areas prone to waterlogging and secondary salinization. 相似文献
3.
Grossnickle SC 《Tree physiology》1988,4(1):85-97
Water relations of bare-root jack pine (Pinus banksiana Lamb.) and white spruce (Picea glauca (Moench) Voss) planted in a greenhouse and on a boreal cut-over site were examined during the first growing season. In field-planted trees, maximum stomatal conductances (g(wv)) were initially low (< 0.10 cm s(-1)). Base and minimum xylem pressure potentials (Psi(x(base)) and Psi(x(min))) were less than -1.5 and -1.7 MPa for jack pine and -2.0 and -2.6 MPa for white spruce, respectively. During the growing season, maximum g(wv) increased in both species to around 0.2 cm s(-1). Base and minimum xylem pressure potentials also increased in both species to around -0.5 and -1.0 MPa in jack pine and -1.0 and -1.5 MPa in white spruce, respectively. Minimum xylem pressure potentials in white spruce fell below the turgor loss point during the first half of the growing season. Osmotic potential at the turgor loss point Psi(pi(TLP)) decreased after field planting to around -2.7 and -2.3 MPa in jack pine and white spruce, respectively. In the greenhouse, minimum values of Psi(pi(TLP)) were -2.2 and -2.3 MPa in jack pine and white spruce, respectively. Maximum bulk modulus of elasticity was greater in white spruce and underwent greater seasonal change than in jack pine. Relative water content (RWC) at turgor loss ranged between 71 and 74% in jack pine and 80 and 87% in white spruce. Available turgor (T(avail)), defined as the integral of turgor over the range of RWC between Psi(x(base)) and xylem pressure potential at the turgor loss point, was similar in jack pine and white spruce just after field planting. For the rest of the growing season, however, T(avail) in jack pine was two to three times that in white spruce. Diurnal turgor (T(diurnal)), defined as the integral of turgor over the range of RWC between Psi(x(base)) and Psi(x(min)), as a percent of T(avail) was higher in field-planted white spruce than jack pine until the end of the season. Dynamics of tissue water potential components are discussed in relation to plantation establishment. 相似文献
4.
Ogren E 《Tree physiology》1999,19(11):749-754
Fast-growing willow clones (six clones of Salix viminalis L. and one clone each of S. viminalis x S. schwerenii E. Wolf and S. dasyclados Wimm.) were compared with respect to growth rhythm and frost hardening in the fall. Frost resistance of stem tissues was assessed by controlled freezing followed by analysis of chlorophyll fluorescence and scoring of visible cambial discoloration. The fluorescence method proved superior to scoring based on visible cambial discoloration because it was more rapid and less subjective, but needed calibration against cambial damage. Frost hardening in mature parts of stems did not start until growth cessation was initiated in the shoot apices, irrespective of whether growth cessation occurred early or late in the fall. Frost resistance varied because of clonal variations in: (1) pre-hardening frost resistance; (2) timing of growth cessation and hence start of frost hardening; and (3) rate of frost hardening. Compared with coastal and southern clones, continental and northern clones started hardening earlier, and a continental clone proceeded through hardening more rapidly at a given temperature. A cross between a continental and coastal clone was intermediate in timing. The pre-hardening frost resistance, however, was unrelated to both growth and frost hardening characteristics. 相似文献
5.
Leaf water relations responses to limited water supply were determined in 7-month-old plants of a dry inland provenance of Eucalyptus argophloia Blakely and in a humid coastal provenance (Gympie) and a dry inland provenance (Hungry Hills) of Eucalyptus cloeziana F. Muell. Each provenance of E. cloeziana exhibited a lower relative water content at the turgor loss point, a lower apoplastic water content, a smaller ratio of dry mass to turgid mass and a lower bulk modulus of elasticity than the single provenance of E. argophloia. Osmotic potential at full turgor and water potential at the turgor loss point were significantly lower in E. argophloia and the inland provenance of E. cloeziana than in the coastal provenance of E. cloeziana. There was limited osmotic adjustment in response to soil drying in E. cloeziana, but not in E. argophloia. Between-species differences in water relations parameters were larger than those between the E. cloeziana provenances. Both E. cloeziana provenances maintained turgor under moderate water stress through a combination of osmotic and elastic adjustments. Eucalyptus argophloia had more rigid cell walls and reached lower water potentials with less reduction in relative water content than either of the E. cloeziana provenances, thereby enabling it to extract water from dryer soils. 相似文献
6.
Water relation parameters of bare-root seedlings of Chinese fir (Cunninghamia lanceolata Hook.)and Masson pine (Pinus massoniana Lamb.)were measured and changes of root growth potential as well as field survival rate of both species were studied after the bare-root seedlings were exposed in a sunny field condition.the results showed that masson pine had a lower osmotic potential(-2.07Mpa) at turgor loss point and at full turgor(-1.29Mpa),compared with Chinese fir(-1.80Mpa and -1.08Mpa respectively).The parameter Vp/Vo(63.27%) of Masson pine was higher than that of chinese fir (58.03%).This means that Masson pine has a stronger ability to tolerate desiccation,compared to Chinese fir according to analysis of above water relation parameters.Root growth potential and field survival rate decreased with prolonging duration of exposure.The field survival rate of both species was reduced to less than 40% after the seedling being exposed only two hours.Water potentials of -1.60 Mpa and -1.70 Mpa were suggested to be critical values for Chinese fir and Masson pine respectively in successful reforestation. 相似文献
7.
Changes in leaf size, specific leaf area (SLA), transpiration and tissue water relations were studied in leaves of rooted cuttings of selected clones of Eucalyptus globulus Labill. subjected to well-watered or drought conditions in a greenhouse. Significant differences between clones were found in leaf expansion and transpiration. There was a significant clone x treatment interaction on SLA. Water stress significantly reduced osmotic potential at the turgor loss point (Pi0) and at full turgor (Pi100), and significantly increased relative water content at the turgor loss point and maximum bulk elastic modulus. Differences in tissue water relations between clones were significant only in the mild drought treatment. Among clones in the drought treatments, the highest leaf expansion and the highest increase in transpiration during the experiment were measured in those clones that showed an early and large decrease in Pi0 and Pi100. 相似文献
8.
Ogren E 《Tree physiology》1999,19(11):755-760
The accumulation of sugars and dry matter in stems in fall was examined in relation to frost hardening in eight willow clones (six clones of Salix viminalis L. and one clone each of S. viminalis x S. schwerenii E. Wolf and S. dasyclados Wimm.). Evidence is presented that three sources of variation in fall frost resistance among the eight clones could be assessed from an analysis of stem composition. First, the pre-hardening value of frost resistance could be assessed from the total sugar concentration. Second, the start of induction of apical growth cessation and hence frost hardening could be distinguished by a stepwise increase in sucrose-to-glucose ratio. Third, the progress of frost hardening during its first phase could be followed from a proportional rise in total sugar concentration and, even more accurately, from a proportional rise in dry-to-fresh weight ratio. In contrast, the second phase of frost hardening was largely uncoupled from sugar and dry matter accumulation. Raffinose and sucrose accumulation seemed to be under differential environmental controls. Sucrose accumulation started with the initiation of growth cessation controlled by photoperiod, whereas raffinose accumulation started with falling temperatures later on. Starch reserves that built up in stems in early fall were partially mobilized later on to support sugar accumulation. In contrast to stems, leaves did not exhibit a preferential accumulation of sucrose in fall. 相似文献
9.
Pressure-volume curves were determined for black spruce (Picea mariana (Mill.) BSP) trees from four full-sib families. During the first two years, trees were measured from a plantation on a dry site. In the third year, trees were sampled from the dry site and a wet site. Diurnal measurements of shoot water potential allowed in situ shoot turgor to be estimated in addition to standard water relations traits. Over all years, Female 59 progeny displayed lower osmotic potentials at saturation (Psi(sat)) than Female 63 progeny. Genetic differences in Psi(sat) were similar on both the dry and wet sites. Modulus of elasticity (epsilon) was greater for Female 59 progeny than for Female 63 progeny, producing a compensatory effect resulting in no genetic or site differences in osmotic potential at turgor loss point (Psi(tlp)) or relative water content at turgor loss point (RWC(tlp)). Mean and predawn shoot turgor pressures (P(x) and P(pd)) were higher for Female 59 progeny than for Female 63 progeny and higher at the wet site than the dry site. Genotype x environment trends were observed; compared to Female 63 progeny, Female 59 progeny displayed 9.8 and 5.1% higher P(pd) on the dry and wet sites, respectively, and 3.4 and 9.8% greater P(pd) values in wet and dry years, respectively. Tree volume growth showed no relationship to Psi(tlp) or RWC(tlp), but was correlated with Psi(sat) and P(x); however, the strongest correlation was with P(pd) (r = 0.90). 相似文献
10.
Pressure-volume curves and shoot water potentials were determined for black spruce (Picea mariana (Mill.) BSP) trees from four full-sib families at the Petawawa Research Forest, Ontario, Canada. Trees were sampled from a dry site in 1992 and from the dry site and a wet site in 1993. Modulus of elasticity (epsilon), osmotic potential at turgor loss point (Psi(tlp)) and relative water at turgor loss point (RWC(tlp)) all decreased during the growing season. Osmotic potential at saturation (Psi(sat)) and turgor displayed no general temporal trend. Across a range of environmental conditions, Female 59 progeny had equal or lower Psi(sat), and higher or similar epsilon, mean turgor pressure (P(x)) and predawn turgor pressure (P(pd)) compared with Female 63 progeny. Osmotic potential at saturation decreased as water stress increased from mild to moderate and increased as water stress increased from moderate to severe. Stable genetic differences in Psi(sat) were maintained by the same rate of osmotic adjustment from low to moderate water stress. Modulus of elasticity and RWC(tlp) decreased with decreasing water availability, whereas Psi(tlp) showed no response. The combined effects of Psi(sat) and epsilon resulted in no change in P(pd) as water stress increased from low to moderate values, but turgor declined sharply as water stress increased from moderate to high values. We conclude that drought tolerance traits strongly influence the growth of these black spruce families across sites of varying water availability. 相似文献
11.
Bareroot jack pine (Pinus banksiana Lamb.) and white spruce (Picea glauca (Moench) Voss) were planted near Elliot Lake, Ontario, on a boreal reforestation site. Site preparation treatments were mixed, mineral and undisturbed (i.e., control) soil. Seedling water relations and growth were examined during the first field season. During the first 28 days after planting, jack pine base (i.e., predawn) and minimum xylem water potential readings were more negative in the control site preparation treatment. White spruce, during the first 10 days, in all site preparation treatments had base and minimum xylem water potential readings more negative than –1.7 MPa. By day 28 base xylem water potentials of white spruce had increased to approximately –1.0 MPa in all site preparation treatments. As the growing season progressed, white spruce minimum xylem water potential readings ceased exceeding the measured turgor loss point first in the mixed followed by the mineral and then control site preparation treatment. Jack pine minimum xylem water potential readings, in all site preparation treatments, almost never exceeded the measured turgor loss point. Water stress and stomatal optimization integrals, day 28 and 125, for both species showed least water stress and greater stomatal optimization in the mixed, mineral and control site preparation treatments, respectively. Both species had less new root growth in the field during the first 28 days after planting compared to seedlings grown for 28 days in a greenhouse for root growth capacity testing. Root growth at 28 days and both shoot and root development at the end of the growing season, were greatest to least in mixed, mineral, and control site preparation treatments, respectively. 相似文献
12.
《Forest Ecology and Management》1987,18(4):299-318
Bare-root jack pine (Pinus banksiana Lamb.) and white spruce (Picea glauca (Moench) Voss) seedlings were planted on a boreal cut-over site and subsequent growth and seedling water relation patterns were monitored over the first growing season. Comparison of morphological development between white spruce and jack pine showed jack pine seedlings had greater new root development and a lower new shoot/new root ratio, while white spruce seedlings had greater new shoot development. Seasonal water relation patterns showed white spruce seedlings to have a greater decrease in xylem pressure potential (x) per unit increase in transpirational flux density in comparison to jack pine seedlings. These results suggest that the greater resistance to water flow through the soil-plant-atmosphere continuum in white spruce seedlings compared to jack pine seedlings may be due to the relative lack of new root development in white spruce. Stomatal response of the seedlings showed that as absolute humidity deficit between the needles and air (AHD) increased, needle conductance (gwv) decreased in both species, but at low AHD levels white spruce had gwv approximately 35% higher than jack pine. For white spruce seedlings, gwv decreased as x became more negative in a predictable curvilinear manner, while gwv of jack pine seedlings responded to x with a threshold closure phenomenon at approximately - 1.75 MPa. Tissue water potential components for jack pine and white spruce seedlings at the beginning and end of the growing season showed jack pine to reach turgor loss at 76% relative water content while white spruce reached turgor loss at 88% relative water content. White spruce seedlings showed osmotic adjustment over the growing season, with an osmotic potential at turgor loss of - 1.27 MPa and - 1.92 MPa at the beginning and end of the growing season, respectively. Jack pine did not show any osmotic adjustment over the growing season. The implication of morphological development on water relation patterns are discussed with reference to successful seedling establishment. 相似文献
13.
Grossnickle SC 《Tree physiology》1992,11(3):229-240
Freezing tolerance and shoot water relations parameters of western red cedar (Thuja plicata Donn) seedlings were measured every 2 weeks from October 1989 to April 1990. Freezing tolerance, measured by freeze-induced electrolyte leakage, showed seasonal shifts in the temperature causing 50% foliage electrolyte leakage (LT(50)). The LT(50) value was -4 degrees C in October, it decreased to -20 degrees C in February and then increased to -6 degrees C in April. The foliage index of injury at -10 degrees C (II(-10)) also showed seasonal shifts from a high of 98% in October to a low of 18% in February followed by an increase to 82% in April. Osmotic potentials at saturation (Psi(s(sat))) and turgor loss point (Psi(s(tlp))) were, respectively, -1.07 and -1.26 MPa in October, -1.57 and -2.43 MPa in January, and -1.04 and -1.86 MPa in April. Dry weight fraction (DWF) increased and symplastic volume at full turgor (V(o)) decreased during the fall-winter acclimation phase, whereas DWF decreased and V(o) increased during the late winter-spring deacclimation phase. Relationships between seasonal patterns of freezing tolerance and shoot water relations parameters showed that LT(50) and II(-10) decreased linearly as Psi(s(tlp)) and V(o) decreased and DWF increased. There was no discernible difference in the relationship during fall acclimation or spring deacclimation. The freezing dehydration index at -10 degrees C (FDI(-10)) declined from 0.69 in November to 0.41 in February and increased to 0.56 in April. The value of II(-10) decreased linearly as FDI(-10) decreased, although a measurement made on actively growing spring foliage did not fit this relationship. The results indicate that seasonal changes in freezing tolerance of western red cedar are partially due to changes in tissue water content, symplastic volume, passive osmotic adjustment and FDI(-10). 相似文献
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16.
Diurnal variation in leaf extension and biophysical parameters of leaf growth were measured in young leaves from a stand of Salix viminalis L. in southern Sweden over a two-day period of clear skies during late July. Leaf growth rate (irreversible extension) was greatest during the late afternoon and early evening, falling to negligible values during the night and early morning.Leaf water potential and leaf osmotic potential showed declining values in the morning with subsequent recovery in the late afternoon. Diurnal variation in osmotic potential (-1.3 to -1.7 MPa) was small compared with that of leaf water potential (-0.1 to -1.2 MPa). Calculated values of leaf turgor pressure during the night (1.2 MPa) were double the midday values. Growth rate correlated poorly with turgor, which (except on one occasion) was always above a calculated value of yield turgor at 0.53 MPa.Diurnal variation in extension growth rate was large compared with that in plastic extensibility of leaf tissue as measured by an Instron technique. Values of extensibility were low and showed little diurnal variation, which is consistent with a proposed negative feedback of expansive growth rate on extensibility. Extension growth rate correlated well with air temperature, suggesting that the rate of leaf expansion may have been limited by a temperature-dependent rate of cell wall loosening. 相似文献
17.
Eight populations of Sitka spruce (Picea sitchensis (Bong.) Carr.) and interior spruce (Picea glauca (Moench) Voss x Picea engelmannii Parry ex. Engelm.) seedlings were sampled from a zone of Sitka-interior spruce introgression in British Columbia, Canada. Restriction fragment length polymorphisms of the nuclear ribosomal RNA genes (rDNA) were used to define species-specific hybridization patterns for the Sitka spruce and interior spruce populations. Hybridization was estimated from an index based on the relative abundance of polymorphic rDNA combining bands for each population. Sitka x interior hybrid seedlings had an index value for the relative abundance of interior spruce rDNA (Si-rDNA) ranging from 0.07 (Lower Nass; the most westerly collected source) to 0.95 (Bulkley Valley low-elevation seed orchard). During shoot elongation, osmotic potential at saturation (Psi(sat)) and turgor loss point (Psi(tlp)) increased, whereas total turgor (Psi(PTotal)) decreased. After bud set in the summer and throughout the fall, Psi(sat) and Psi(tlp) decreased, whereas Psi(PTotal) increased. At all times of year, populations with a higher Si-rDNA index had lower Psi(tlp) and Psi(sat) and higher Psi(PTotal) than populations with a lower Si-rDNA index. During the fall, Sitka x interior hybrid seedlings exhibited a seasonal decline in the temperature causing 50% needle electrolyte leakage (LT(50)) and in the critical temperature indicating the initial point of freezing injury. Seedlings with a higher Si-rDNA index had lower LT(50) and critical temperature values indicating greater freezing tolerance in the fall. Throughout most of the year, seedling population Si-rDNA index was related to the degree of drought and freezing tolerance. 相似文献
18.
We investigated seasonal patterns of water relations in current-year leaves of three evergreen broad-leaved trees (Ilex pedunculosa Miq., Ligustrum japonicum Thunb., and Eurya japonica Thunb.) with delayed greening in a warm-temperate forest in Japan. We used the pressure-volume method to: (1) assess the extent to which seasonal variation in leaf water relations is attributable to leaf development processes in delayed greening leaves versus seasonal variation in environmental variables; and (2) investigate variation in leaf water relations during the transition from the sapling to the adult tree stage. Leaf mass per unit leaf area was generally lowest just after completion of leaf expansion in May (late spring), and increased gradually throughout the year. Osmotic potential at full turgor (Psi(o) (ft)) and leaf water potential at the turgor loss point (Psi(w) (tlp)) were highest in May, and lowest in midwinter in all species. In response to decreasing air temperature, Psi(o) (ft) dropped at the rate of 0.037 MPa degrees C(-1). Dry-mass-based water content of leaves and the symplastic water fraction of total leaf water content gradually decreased throughout the year in all species. These results indicate that reductions in the symplastic water fraction during leaf development contributed to the passive concentration of solutes in cells and the resulting drop in winter Psi(o) (ft). The ratio of solutes to water volume increased in winter in current-year leaves of L. japonicum and E. japonica, indicating that osmotic adjustment (active accumulation of solutes) also contributed to the drop in winter in Psi(o) (ft). Bulk modulus of elasticity in cell walls fluctuated seasonally, but no general trend was found across species. Over the growing season, Psi(o) (ft) and Psi(w) (tlp) were lower in adult trees than in saplings especially in the case of I. pedunculosa, suggesting that adult-tree leaves are more drought and cold tolerant than sapling leaves. The ontogenetic increase in the stress resistance of I. pedunculosa may be related to its characteristic life form because I. pedunculosa grows taller than the other species studied. 相似文献
19.
Abrams MD 《Tree physiology》1988,4(3):263-273
Water relations of co-occurring understory saplings of Quercus ellipsoidalis E.J. Hill, an early successional, xeric species, Populus tremuloides Michx., an early successional, mesic species, and Acer rubrum L., a late successional species that occurs on both wet and dry sites, were evaluated on four dates during the 1986 growing season. The understory was characterized by high soil water content, low irradiance and low vapor pressure deficit throughout the growing season. Stomatal conductance and calculated transpiration flux were lowest for A. rubrum and highest for P. tremuloides and Q. ellipsoidalis. Except early in the growing season, leaf water potentials were lower in P. tremuloides than in the other species. Populus tremuloides had the highest bulk modulus of elasticity, Q. ellipsoidalis the lowest. Over the growing season, Populus tremuloides and Q. ellipsoidalis, but not A. rubrum, exhibited a decrease in osmotic potential at both full and zero turgor. Of the three species, Populus tremuloides exhibited the sharpest decrease in leaf water potential and turgor pressure with decreasing relative water content. 相似文献
20.
Villar-Salvador P Planelles R Oliet J Peñuelas-Rubira JL Jacobs DF González M 《Tree physiology》2004,24(10):1147-1155
Drought stress is the main cause of mortality of holm oak (Quercus ilex L.) seedlings in forest plantations. We therefore assessed if drought hardening, applied in the nursery at the end of the growing season, enhanced the drought tolerance and transplanting performance of holm oak seedlings. Seedlings were subjected to three drought hardening intensities (low, moderate and severe) for 2.5 and 3.5 months, and compared with control seedlings. At the end of the hardening period, water relations, gas exchange and morphological attributes were determined, and survival and growth under mesic and xeric transplanting conditions were assessed. Drought hardening increased drought tolerance primarily by affecting physiological traits, with no effect on shoot/root ratio or specific leaf mass. Drought hardening reduced osmotic potential at saturation and at the turgor loss point, stomatal conductance, residual transpiration (RT) and new root growth capacity (RGC), but enhanced cell membrane stability. Among treated seedlings, the largest response occurred in seedlings subjected to moderate hardening. Severe hardening reduced shoot soluble sugar concentration and increased shoot starch concentration. Increasing the duration of hardening had no effect on water relations but reduced shoot mineral and starch concentrations. Variation in cell membrane stability, RT and RGC were negatively related to osmotic adjustment. Despite differences in drought tolerance, no differences in mortality and relative growth rate were observed between hardening treatments when the seedlings were transplanted under either mesic or xeric conditions. 相似文献