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1.
The effect of the dual inoculation with arbuscular mycorrhizal (AM) and saprophytic fungi and a combination of wheat straw and sewage sludge residues were studied by determining their effect on dry weight of tomato and on chemical and biochemical properties of soil. Incubation of organic residue (sewage sludge combined with wheat straw) with saprophytic fungi and plant inoculation with mycorrhizal fungi was essential to study plant growth promotion. Soil application of organic residues increased the dry weight of tomato inoculated with Rhizophagus irregularis. The greatest shoot dry mass was obtained when the organic residues were incubated with Trichoderma harzianum and applied to AM plants. However, the greatest percentage of root length colonized with AM in the presence of the organic residues was obtained with inoculation with Coriolopsis rigida. The relative chlorophyll was greatest in mycorrhizal plants regardless of the presence of either saprophytic fungus. The presence of the saprophytic fungi increased soil pH as the incubation time increased. Soil nitrogen and phosphorus contents and acid phosphatase were stimulated by the addition of organic residues, and contents of N and P. Total N and P content in soil increased when the organic residue was incubated with saprobe fungi, but this effect decreased as the incubation period of the residue with saprobe fungi increased. The same trend was observed for soil β‐glucosidase and fluorescein diacetate activities. The application of organic residues in the presence of AM and saprophytic fungi seems to be an interesting option as a biofertilizer to improve plant growth and biochemical parameters of soils.  相似文献   

2.
Soil-borne plant pathogens are among the most important limiting factors for the productivity of agro-ecosystems.Fungistasis is the natural capability of soils to inhibit the germination and growth of soil-borne fungi in the presence of optimal abiotic conditions.The objective of this study was to assess the effects of different soil managements,in terms of soil amendment types and frequency of application,on fungistasis.For this purpose,a microcosm experiment was performed by conditioning a soil with frequent applications of organic matter with contrasting biochemical quality (i.e.,glucose,alfalfa straw and wheat straw).Thereafter,the fungistasis response was assessed on four fungi (Aspergillus niger,Botrytis cinerea,Pyrenochaeta lycopersici and Trichoderma harzianum).Conditioned soils were characterized by measuring microbial activity (soil respiration) and functional diversity using the BIOLOG EcoPlatesTM method.Results showed that irrespective of the fungal species and amendment types,frequent applications of organic matter reduced fungistasis relief and shortened the time required for fungistasis restoration.The frequent addition of easily decomposable organic compounds enhanced soil respiration and its specific catabolic capabilities.This study demonstrated that frequent applications of organic matter affected soil fungistasis likely as a result of higher microbial activity and functional diversity.  相似文献   

3.
Summary Studies using cocultures of a cellulolytic fungus with a capsular organism show that microbial polysaccharides can be synthesized during the degradation of wheat straw. Polysaccharide accounted for up to 1.6% (w/w) of the initial straw weight but the sugar composition and viscosity indicated that a large fraction was low molecular weight material of plant origin. Nevertheless several cocultures produced sufficient microbial polysaccharide effectively to stabilize aggregates of Hamble silt loam. This correlated significantly with increased viscosity and also stabilization of Mount St. Helens volcanic ash. Four cocultures, Sordaria alcina with two strains of Enterobacter cloacae and a Pseudomonas sp. and Trichoderma harzianum with E. cloacae, were significantly more effective than a natural inoculum from straw. Inoculation of straw to produce a compost which can increase the stability of poorly structured soils is suggested.  相似文献   

4.
In a simple experimental system with wheat plants, interactions between the collembolan Onychiurus armatus and Trichoderma harzianum, a soil-borne fungus parasite of several plant pathogenic fungi, were studied in the presence of Gaeumannomyces graminis var. tritici, one of the most important foot and root fungal pathogens of cereals world-wide. Trichoderma harzianum was used according to two different modalities: fungal inoculum applied to seed or mixed with substrate. The isolate of T. harzianum proved to be ineffective against the pathogenic fungus, independent of the application modality, as shown in the experiments where this fungus was used alone, whereas Collembola used alone significantly reduced disease severity. However, the mode by which T. harzianum was applied significantly influenced the disease control ability of Collembola. In fact, only when T. harzianum was coated to seed were springtails able to reduce the disease caused by G. graminis var. tritici.  相似文献   

5.
Inoculation of wheat and corn grains with formulations of Azospirillum brasilense significantly increased seedling growth parameters of wheat and corn compared to untreated controls. Inoculation with Azospirillum and supplemental Trichoderma harzianum free or coimmobilized in calcium alginate resulted in significant increase in all plant growth parameters in addition to improving plant nutrient-content [phosphorus (P), potassium (K), and calcium (Ca)]. Grain treatments with T. harzianum alone or in a combination with A. brasilense were protected from invasion by Fusarium in a pot experiment. Nitrogen (N) fixation was investigated by A. brasilense free or double inoculated with T. harzianum in soil amended with different C-sources; also, phosphate solubilization was tested by these two organisms. Single and double inoculation with A. brasilense and/or T. harzianum improved wheat yield growth parameters in addition to seed protein; therefore, immobilized and coimmobilized formulations could be used as biofertilizer and biopesticide, and might be recommended to avoid the extensive use of the agrochemicals.  相似文献   

6.
This study was conducted to investigate the effect of Trichoderma harzianum (T22) on the growth and development of maize (Zea mays) plants. T. harzianum was applied to the grains in two different treatments, either by inoculating the soil with air-dried mycelia of T. harzianum or by treating the seeds with different concentrations of the metabolic solution (as the culture supernatant of T. harzianum) before sowing. Comparisons were made between the two treatments to determine if any beneficial effects for improving the growth of maize plants occurred. Results revealed that application of the highest concentration of air-dried mycelia and all concentrations of the metabolic solution of T. harzianum caused increases in all measured parameters which include growth parameters, chlorophyll content, starch content, nucleic acids content, total protein content and phytohormone content of maize plants but the magnitude of these increases was much more pronounced in case of treating the seeds with the metabolic solution of T. harzianum.  相似文献   

7.
To better understand the role of organic matter （OM） prepared from chicken manure and agriculture residues compost on the growth of plants （Lepidium sativum L.） and antagonistic fungi （Trichoderma harzianum）, we analyzed the structure and composition of extracted OM using fluorescence excitation-emission matrix （EEM） spectroscopy and solid-state 13C cross-polarization magic-angle- spinning nuclear magnetic resonance （13C CPMAS NMR） spectroscopy. The results showed that the EEM contours of water-extracted OM （WEOM） and alkali-extracted OM （AEOM） were similar. Furthermore, solid-state 13C CPMAS NMR spectroscopy demonstrated that water extraction could not proportionally pull out aromatic moieties （112-145 ppm） from compost, but the alkali method in proportion extracted both carbohydrates （65 85 ppm） and aromatic moieties. The results suggest that AEOM may better reflect the bulk OM composition of compost, and one should be cautious when applying WEOM as an alternative indicator of total compost OM. Further investigations demonstrated that, compared to carbohydrates, aromatic moieties played a predominant role in growth suppression of Lepidium sativum L. seeds and Trichoderma harzianum.  相似文献   

8.
Rose flower is one of the most important cut flowers, with the world's highest production rate. Soilless culture has been increasingly popular in plant production worldwide in the past few decades. In two separate trials, the efficacy of Trichoderma harzianum, Anearobacillus migululanas, slow filtration, and UV ultraviolet (UV) treatment and sand filtration in eliminating Fusarium oxyporum, artificially added in the recirculating nutrient solution, was evaluated with Rosea hybrid plants grown in closed soilless systems. The dynamics of these fungi in the recirculating nutrient solution and in the sand filter was also investigated. T. harzianum, A. migululanas, UV treatment and slow sand filtration were effective in reducing Fusarium root rot. However, slow sand filtration may be a more feasible disinfection method than UV because of lower costs of installation and maintenance and for its adaptability to a wide range of production systems. Moreover, these disinfection techniques can be successfully combined with the application of antagonistic microorganisms.  相似文献   

9.
The filamentous fungi Trichoderma spp. is currently developed as biocontrol agents against many plant pathogens. Recent studies have shown that these fungi are able to infect nematode eggs and juveniles. In this research, biological control of root-knot nematode (Meloidogyne javanica) by Trichoderma harzianum BI was investigated in greenhouse and laboratory experiments. Results showed that different concentrations (102–108 spores/ml) of T. harzianum BI decreased nematode infection and other parameters significantly, compared to control. T. harzianum BI was able to penetrate nematode egg mass matrix and significantly decreased nematode egg hatching level. Specific activities of resistance-related enzymes, namely peroxidase (POX), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) increased significantly in T. harzianum BI inoculated plants. Maximum activities of POX, PPO and PAL were observed at the 5, 5 and 6 days after inoculation, respectively. Chitinase activity was also increased in culture filtrates of T. harzianum BI grown on wheat bran moistened with salt solution supplemented with colloidal chitin or nematode eggs. Maximum activity of chitinase was recorded at the 4 days after inoculation, in media supplemented with colloidal chitin (1.15 U/min per ml) and nematode eggs (0.85 U/min per ml). Results suggested that direct parasitism of eggs through the increase in extracellular chitinase activity, which would be indicator of eggs infection capability, and inducing plant defense mechanisms leading to systemic resistance are two main suppression mechanisms used by T. harzianum BI against nematode.  相似文献   

10.
Strigolactones have recently been suggested to be phytohormones that are present in all plants. Strigolactones are released by roots into the rhizosphere, stimulating the seed germination of parasitic plants such as Striga spp. and Orobanche spp. and play a crucial role in the interaction between plants and symbiotic arbuscular mycorrhizal fungi.By applying different concentrations of the synthetic strigolactone analogue GR24 to alfalfa (Medicago sativa) inoculated with Sinorhizobium meliloti we could show that in alfalfa nodulation is positively affected by the presence of the strigolactone analogue GR24. Moreover, we could show that this increased nodulation cannot be linked with a stimulatory effect of GR24 on the growth or the expression of nod genes of S. meliloti.Putative mechanisms operating in the plant in response to the addition of GR24 and leading to increased nodule formation by rhizobia are discussed.  相似文献   

11.
The application of paddy straw to soil improved the yield of soybeans significantly in a light-textured red sandy loam soil with a pH of 5.6. Whereas the nodulation was unaffected in the crop grown under rainfed conditions it was improved in the irrigated summer crop of soybeans. Grain yield was significantly increased in treatment receiving straw at 3 t/ha. Addition of dung slurry as a source of inoculum for straw decomposition or a second inoculation of plants with Rhizobium failed to affect nodulation and yield of soybeans.  相似文献   

12.
The effect of salinity on the efficacy of two arbuscular mycorrhizal fungi, Glomus fasciculatum and G. macrocarpum, alone and in combination was investigated on growth, development and nutrition of Acacia auriculiformis. Plants were grown under different salinity levels imposed by 0.3, 0.5 and 1.0 S m-1 solutions of 1 M NaCl. Both mycorrhizal fungi protected the host plant against the detrimental effect of salinity. The extent of AM response on growth as well as root colonization varied with fungal species, and with the level of salinity. Maximum root colonization and spore production was observed with combined inoculation, which resulted in greater plant growth at all salinity levels. AM fungal inoculated plants showed significantly higher root and shoot weights. Greater nutrient acquisition, changes in root morphology, and electrical conductivity of soil in response to AM colonization was observed, and may be possible mechanisms to protect plants from salt stress.  相似文献   

13.
The accumulation of phenolic acids in soil is one of the main problems associated with continuous cropping of peanut. Although laccases secreted by fungi can efficiently transform phenolic acids, there are few reports on the use of these enzymes to bioremediate continuous cropping soil. Food waste and wheat straw are waste products; however, they could be used productively as resources for laccase production by the endophytic fungus Phomopsis liquidambari B3. We cultured Phomopsis liquidambari B3 in medium containing food waste as the main nitrogen source and wheat straw as the main carbon source. In order to study the effects of fermentation liquid on phenolic acid degradation, rhizosphere soil microbial communities and peanut seedling growth, the fermentation product, which had high laccase activity, was added to continuously cropped soil of peanut. The concentration of 4-hydroxybenzoic acid, vanillic acid, and coumaric acid in soil had decreased by 57.4, 52.5, and 49.4%, respectively, compared with no-treatment control during 28 days. Analysis of denaturing gradient gel electrophoresis profiles showed that the bacterial and fungal community structures in rhizosphere soil were affected by changes in the phenolic acids concentration. The biomass of peanut plants and the number of root nodules were increased 68.3% and 5.9-fold, respectively. These results showed that the laccase product reduced the accumulation of phenolic acids in soil, the decrease in phenolic acids concentration and the increase in certain dominant microorganisms promoted peanut seedling growth and nodulation. This technology provides a new strategy for bioremediation of continuous cropping soil, while simultaneously reducing waste and protecting the environment.  相似文献   

14.
Effects of heavy metals on rhizobia and the symbiotic association with leguminous hosts are currently unclear. To investigate this problem, we examined Rhizobium meliloti (microsymbiont) and alfalfa (Medicago sativa) (macrosymbiont) collected from soils contaminated with varying concentrations of heavy metals (varying distances from a Zn smelter operating 90 yr.). Soil populations of R. meliloti were not correlated with metal concentrations in soil. The lowest rhizobial population was found in the soil with the highest extractable metal concentrations, but the highest populations were found in soil which was moderately contaminated. A greenhouse study in which alfalfa was grown in the same soils showed no significant trend for nodulation or nitrogenase activity of roots. Highest nodule number and nitrogenase activity were observed in those soils which had the lowest population of R. meliloti. When the heavy metal Minimum Inhibitory Concentration (MIC) of individual isolates was examined, no correlation was found between the MIC and soil metal concentration (total, or water or 0.01 M Ca(NO3)2 extractable).These results indicate that even in highly contaminated soils, metal activity was not high enough to exert an antagonistic influence on the soil rhizobial population or the symbiotic association between alfalfa and R. meliloti.  相似文献   

15.
 Take-all and brown foot rot, caused respectively by Gaeumannomyces graminis var. tritici and Fusarium culmorum, are two important components of the foot and root fungal disease complex of winter cereals world-wide. These fungi persist in soil and in crop debris in the same layer of agricultural soil as Collembola, a well represented taxon of soil animals. Previous in vitro tests showed that these fungi grown on agarised medium were readily consumed by springtails. In a simplified experimental system with wheat plants and the pathogenic fungi grown on millet and wheat kernels, the severity of disease was significantly reduced by collembolan feeding activity. Received: 18 April 2000  相似文献   

16.
Representatives of the same species of microscopic fungi developed on composite materials with similar polymeric matrices independently from the type of soils, in which the incubation was performed. Trichoderma harzianum, Penicillium auranthiogriseum, and Clonostachys solani were isolated from the samples of polyurethane. Fusarium solani, Clonostachys rosea, and Trichoderma harzianum predominated on the surface of ultrathene samples. Ulocladium botrytis, Penicillium auranthiogriseum, and Fusarium solani predominated in the variants with polyamide. Trichoderma harzianum, Penicillium chrysogenum, Aspergillus ochraceus, and Acremonium strictum were isolated from Lentex-based composite materials. Mucor circinelloides, Trichoderma harzianum, and Penicillium auranthiogriseum were isolated from composite materials based on polyvinyl alcohol. Electron microscopy demonstrated changes in the structure of polymer surface (loosening and an increase in porosity) under the impact of fungi. The physicochemical properties of polymers, including their strength, also changed. The following substances were identified as primary products of the destruction of composite materials: stearic acid for polyurethane-based materials; imide of dithiocarbonic acid and 1-nonadecen in variants with ultrathene; and tetraaminopyrimidine and isocyanatodecan in variants with polyamide. N,N-dimethyldodecan amide, 2-methyloximundecanon and 2-nonacosane were identified for composites on the base of Lentex A4-1. Allyl methyl sulfide and imide of dithiocarbonic acid were found in variants with the samples of composites based on polyvinyl alcohol. The identified primary products of the destruction of composite materials belong to nontoxic compounds.  相似文献   

17.
Micronutrient deficiency and malnutrition in humans are severe problems in many developing countries, particularly in areas with calcareous soils. There is almost no information on whether inoculation with plant growth–promoting Azospirillum and/or Trichoderma can help to reduce this problem by increasing the mineral concentration of the seeds. Field experiments were conducted in Tokat (Turkey) in 2001–2002 to determine whether inoculation with Azospirillum brasilense, Trichoderma harzianum, sole or in combination, and/or the application of P fertilizers can enhance micronutrient concentrations of field‐grown bean (Phaseolus vulgaris) and wheat (Triticum aestivum). In beans, Azospirillum inoculation combined with P fertilization significantly (p < 0.05) increased seed concentrations of Mn, Zn, and Cu, from 8.8, 22.6, and 7.0 mg kg–1 in the control to 10.3, 28.3, and 11.0 mg kg–1, respectively. Trichoderma inoculation alone significantly (p < 0.05) reduced the concentrations of Fe, Mn, Zn, and Cu and the cumulative plant uptake of Fe and Zn in 45‐day‐old bean plants. However, it significantly (p < 0.05) increased bean‐seed Cu content and accumulation. The double inoculation resulted in significantly (p < 0.05) higher micronutrient concentrations than Trichoderma inoculation alone in 45‐day‐old plants. In contrast to beans, the effects of microbial inoculations were less in wheat. However, dual inoculation significantly (p < 0.05) increased Zn content by 45% and Zn accumulation by 40% above the uninoculated control. Inoculation with plant growth–promoting microorganisms appears to be a promising strategy to combat micronutrient deficiencies.  相似文献   

18.
Plant disease resistance and susceptibility are greatly influenced by the availability of micronutrients, particularly manganese (Mn) and zinc (Zn). Take-all disease of wheat, caused by a strong Mn oxidizing fungus (Gaeumannomyces graminis var tritici, Ggt), results in a lack of availability of Mn to plants and increases disease severity in wheat. Three commercial Trichoderma harzianum (Vitalin T-50, BioHealth®-WSG, and BioHealth®-G) and one Bacillus subtilis (Vitalin SP-11) were investigated individually and in combination (Vitalin T-50 and Vitalin SP-11) for growth promotion and Mn/Zn uptake of take-all infected wheat in a rhizobox experiment under greenhouse conditions. Inoculation with Trichoderma and Bacillus biofertilizers did not increase the shoot dry weight and shoot to root ratio, whilst shoot length was significantly increased with Vitalin T-50 and Biohealth-G treatments in the final harvest. Biofertilizers inoculation that significantly (P < 0.05) enhanced root surface area and root dry weight were Vitalin T-50, BioHelath-G and combination of Vitalin (T-50 + SP-11). The bulk soil pH was not influenced by biofertilizer inoculation, whereas rhizosphere and rhizoplane soil pH were significantly reduced (0.3 – 0.4 pH scale) in Vitalin (T-50 + SP-11) and BioHealth-G treatments and to a lesser extent by Vitalin T-50 inoculation. Manganese uptake in shoots of wheat exhibited no significant differences among the biofertilizer treatments. On the contrary, Zn uptake was significantly higher in Vitalin T-50, Vitalin (T-50 + SP-11), BioHealth-G, and BioHealth-WSG (47, 64, 44, and 45%, respectively) inoculated plants. Therefore, Vitalin T-50 and Biohealth-G showed better performance in improving plant growth and Zn uptake.  相似文献   

19.
Residues from some tree species may contain allelopathic chemicals that have the potential to inhibit plant growth and symbiotic N2-fixing microorganisms. Soybean [Glycine max (L.) Merr] was grown in pots to compare nodulation and N2-fixation responses of the following soil amendments: control soil, leaf compost, red oak (Quercus rubra L.) leaves, sugar maple (Acer saccharum Marsh) leaves, sycamore (Platanus occidentalis L.) leaves, black walnut (Juglans nigra L.) leaves, rye (Secale cereale L.) straw, and corn (Zea mays L.) stover. Freshly fallen leaves were collected from urban shade trees. Soil was amended with 20 g kg-1 air-dried, ground plant materials. Nodulating and nonnodulating isolines of Clark soybean were grown to the R2 stage to determine N2-fixation by the difference method. Although nodulation was not adversely affected, soybean grown on leaf-amended soil exhibited temporary N deficiency until nodulation. Nodule number was increased by more than 40% for soybean grown on amended soil, but nodule dry matter per plant generally was not changed compared with control soil. Nonnodulating plants were severely N deficient and stunted as a consequence of N immobilization. Nodulating soybean plants grown on leaf or crop residue amended soil were more dependent on symbiotically fixed N and had lower dry matter yields than the controls. When leaves were composted, the problem of N immobilization was avoided and dry matter yield was not reduced. No indication of an allelopathic inhibition on nodulation or N2-fixation from heavy application of oak, maple, sycamore, or walnut leaves to soil was observed.  相似文献   

20.
Aspergillus tubingensis and A. niger were isolated from the landfills of rock phosphate mines and tested for their efficacy to solubilize rock phosphate (RP), and improve plant growth and phosphate (P) uptake by plants grown in soil amended with RP. The results showed that they effectively solubilized RP in Pikovskaya's (PKV) liquid medium and released significantly higher amounts of P into the medium. A. tubingensis solubilized and released 380.8 μg P mL?1, A. niger showed better efficiency and produced 403.8 μg P mL?1. Field experiments with two consecutive crops in alkaline agricultural soil showed that inoculation of these fungi along with RP fertilization significantly increased yield and nutrient uptake of wheat and maize plants compared with control soil. P uptake by wheat and maize plants and the available P increased significantly in the RP-amended soil inoculated with fungi compared with control. These results suggest that the fertilizer value of RP can be increased, especially in alkaline soils, by inoculating P-solubilizing fungi.  相似文献   

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