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1.
Mining wastes may pose risk nearby urban and agricultural areas. We investigated a lettuce crop land close to a former capped mine tailing in order to determinate the metal uptake by crops. Soil plot sampling design within the crop area and two transects along the tailing were performed. In addition, lettuces (root and leaves) were analyzed after transplant and harvest. The results showed a pH of around 7–8 for all the soil samples. Total metal concentrations were as follows: 190–510 mg kg?1 Pb, 13–21 mg kg?1 Cu, and 210–910 mg kg?1 Zn. Diethylene triamine pentaacetic acid-extractable Pb was around 18% of the total Pb in some samples. Transects along the base and on the plateau of the tailing showed high metal concentrations of Pb (up to 5,800 mg kg?1) and Zn (up to 4,500 mg kg?1), indicating that capping layer had been eroded. Lettuce leaves showed Pb concentrations within standard for human health (<0.3 mg kg?1 in fresh weight). For essential micronutrients such as Cu and Zn, leaves had optimal content (10–28 mg kg?1 Cu, 60–85 mg kg?1 Zn). A continued monitoring in metal uptake is needed in crop lands close to mining wastes in order to prevent risks in food safety. Capped tailings must be monitored and rehabilitation works performed from time to time.  相似文献   

2.
The influence of differing soil management practices on changes seen in soil organic carbon (SOC) content of loamy Haplic Luvisol was evaluated. The field experiment included two types of soil tillage: 1. conventional tillage (CT) and 2. reduced tillage (RT) and two treatments of fertilization: 1. crop residues with nitrogen, phosphorus, and potassium (NPK) fertilizers (PR+NPK) and 2. NPK fertilizers (NPK). The results of SOC fluctuated from 9.8 to 14.5 g kg?1 and the tillage systems employed and fertilization status did not have a statistically significant influence on SOC. The SOC content was higher in RT (12.4 ± 0.86 g kg?1) than in CT (12.2 ± 0.90 g kg?1). On average, there was a smaller higher value of SOC in PR+NPK (12.4 ± 1.02 g kg?1) than in NPK (12.3 ± 0.88 g kg?1). During a period of 18 years, reduced tillage and application of NPK fertilizers together with crop residues build up a SOC at an average speed of 7 and 16 mg kg?1 year?1, respectively, however conventional tillage and NPK fertilizer applications caused a SOC decline at an average speed of 104 and 40 mg kg?1 year?1, respectively.  相似文献   

3.
A pot experiment evaluated the growth of lettuce (Lactuca sativa L.) and barley (Hordeum vulgar) and accumulation of molybdenum (Mo) in plants and soils following amendments of Mo compost (1.0 g kg?1) to a Truro sandy loam. The treatments consisted of 0 (control), 12.5, 25, and 50% Mo compost by volume. The Mo compost did not affect dry‐matter yield (DMY) up to 25% compost, but DMY decreased at the 50% compost treatment. The 50% compost treatments increased the soil pH an average of 0.5 units and increased the nitric acid (HNO3)–extractable Mo to 150 mg kg?1 and diethylenetriaminepentaacetic acid (DTPA)–extractable Mo to 100 mg kg?1 in the growth medium; the same treatment increased tissue Mo concentration to 569 and 478 mg kg?1 in the lettuce and barley, respectively. Plants grown in the 25% compost produced about 55 mg kg?1 of total Mo in the growth medium; this resulted in tissue Mo concentration of 348 mg kg?1 in lettuce and 274 mg kg?1 in barley without any phytotoxicity. Our results suggested that 55 mg Mo kg?1 soil would be an appropriate limit for Mo loading of soil developed from compost additions, a value which is presently greater than the Canadian Council for Ministers of the Environment (CCME) Guidelines for the use of type B compost in Canada.  相似文献   

4.
Rice, dry bean, corn, and soybean are important food crops. Phosphorus (P) deficiency is one of the most yield-limiting factors for these crops grown on highly weathered Brazilian Oxisols. Four greenhouse experiments were conducted to determine P requirements of these four crops. The P levels used were 0, 50, 100, 200, and 400 mg kg?1. Growth, yield, and yield components evaluated of four crop species were significantly increased with the application of P fertilization. Most of the responses were quadratic in fashion when the P was applied in the range of 0 to 400 mg kg?1. Maximum grain yield of upland rice was obtained with the application of 238 mg P kg?1 of soil, maximum dry bean grain yield was obtained with the application of 227 mg P kg?1 of soil, and maximum grain yield of soybean was obtained with the application of 224 mg P kg?1 of soil. Maximum shoot growth of corn was obtained with the addition of 323 mg P kg?1 of soil. Most of the growth and yield components had significant positive association with grain yield or shoot dry weight. Phosphorus concentration and uptake were greater in the grain compared to straw in upland rice and dry bean plants. Overall, P-use efficiencies decreased with increasing P rates.  相似文献   

5.
Potassium humate (PH) is a promising natural resource to be utilized as an alternative for increasing crop production. A pot experiment was conducted during 2009 and 2010 to assess the efficacy of application of potassium humate (0, 5, and 10 mg kg?1 soil) alone and in combination with chemical fertilizers (75% and 100% recommended dose of nitrogen–phosphorus–potassium) on yield and nutrient availability patterns in soil at different growth stages of rice. Two doses of zinc, viz. 0 and 12.5 mg kg?1, were also applied. Sole and combined application of potassium humate with nitrogen–phosphorus–potassium (NPK) and zinc significantly (p < 0.05) improved the yield and availability of nitrogen, phosphorus, potassium, sulfur, zinc, and dehydrogenase activity in soil. Application of 10 mg kg?1 potassium humate along with 100% NPK and 12.5 mg kg?1 zinc sulfate proved significantly superior when compared to 75% and 100% of NPK alone.  相似文献   

6.
Available iron, zinc, copper and manganese were determined in six pedons located in upper slope, middle slope and valley bottom soils derived from Abeokuta geological materials in Nigeria. The soils had an average of 639.8 g kg?1 sand, 241.8 g kg?1 clay and 118.4 g kg?1 silt. The fertility status of the soils was low–medium with a strongly acid–neutral reaction, 1.3–15.1 g kg?1 organic carbon contents, moderate–high exchangeable bases and 1.38 mg kg?1 available phosphorus. Both Fe (122.50 mg kg?1) and Mn (111.40 mg kg?1) occurred at toxic levels, whereas the mean Cu (1.27 mg kg?1) and Zn (2.56 mg kg?1) contents were found to be adequate for most crops grown in the region. There were significant positive correlations among the micronutrients and also between soil pH, organic carbon, particle size fractions and micronutrients. The high levels of Fe and Mn were probably due to the presence of oolitic ironstone in the parent material.  相似文献   

7.
Berpura alluvial soil series of the Indo‐Gangetic Plains is situated in the Ambala District of the Haryana State of India. Soils of this series had medium concentrations of both potassium (K) and phosphorus (P) and large concentrations of sulfur (S) before 1970. To study different fractions of K, Olsen P, and 0.15% calcium chloride (CaCl2)–extractable (available) S of soils of the Berpura series and to create nutrient indexing of rice crops growing on this series, surface soil samples were collected from 100 farmers' fields after the harvest of the wheat crop in 2005. During kharif season of same year, samples of upper two leaves at anthesis growth stage of rice crop were also collected from the same 100 farmers' fields that had earlier been sampled for soil analysis. Analysis of soil samples showed more K depletion in soils of this series, of which 86% of farmers' fields were deficient in ammonium acetate (NH4OAc) K (available K). Thirty and 62% of leaf samples of the rice crop growing on the 100 fields of this series were extremely and moderately deficient in K, respectively. The mean values of water‐soluble, exchangeable, nonexchangeable, lattice, and total K were 10.6, 30.3, 390.0, 8204, and 8635 mg kg?1, respectively. In soils of this series, 0.123, 0.351, 4.517, and 95.009% of total K were found in water‐soluble, exchangeable, nonexchangeable, and lattice K forms, respectively. On the other hand, long‐term farmers' practice of more application of P fertilizer in wheat crop has resulted in P buildup in the soils of the Berpura series. Olsen P in soils of farmers' fields of this series ranged from 9.0 to 153.0 mg kg?1, with the mean value of 41.8 mg kg?1. Eighty‐two percent of leaf samples of rice crops grown on this series without application of P fertilizer were sufficient in P. The analysis of soil and rice crops for P and K proved the suitability of 0.5 M sodium bicarbonate (NaHCO3) and 1 N NH4OAc for extracting available P and K, respectively, in alluvial soils of the Indo‐Gangetic Plains. The 0.15% CaCl2–extractable S in this soil ranged from 9.6 to 307 mg kg?1 with a mean value of 34.6 mg kg?1. Four and 26% of soil samples had low and medium, respectively, in 0.15% CaCl2–extractable S. S deficiency was recorded in rice crops, as 29% of the leaf samples were extremely deficient in S and 58% were moderately deficient in S. This indicated the unsuitability of the 0.15% CaCl2 to extract available S from the Udic ustochrept utilized for cultivation of rice crops.  相似文献   

8.
The distribution in soil and plant uptake of zinc (Zn) and lead (Pb) as influenced by pine bark-goat manure (PBG) compost additions were investigated from the soils artificially contaminated with Zn or Pb ions using maize (Zea mays L.) as a test crop. Soils were amended with four rates of pine bark-goat manure compost (0, 50, 100, and 200 tons ha?1) and four rates (0, 300, 600 and 1200 mg kg?1) of Zn or Pb. Maize was planted and grown for 42 days. At harvest, plants samples were analyzed for Zn and Pb concentration. Soils samples were analyzed for pH, extractable and diethylene triamine pentaacetic acid (DTPA) extractable Zn and Pb. Extractable Zn and Pb was lower in PBG compost amended soils than in unamended soils and steadily declined with increasing amount of compost applied. The extractable fraction for Zn dropped by 62.2, 65.0 and 44.6% for 300, 600 and 1200 mg Zn kg?1, respectively when 200 t ha?1 of PBG compost was applied. Metal uptake by maize plants were directly related to the rate of applied heavy metal ions with greater concentrations of metals ions found where metal ions were added to non-amended soils.  相似文献   

9.
Hydroponic and pot experiments were conducted to assess the uptake of heavy metals (Cd and Zn) by a common crop plant, African basil, Ocimum gratissimum. In addition, the effects of soil amendments, hydroxyapatite (HA) and cow manure on plant growth and metal accumulations were compared. In the hydroponic study, plants were exposed to various concentrations of Cd (2.5 and 5 mg L?1) and Zn (10 and 20 mg L?1) for 15 days. O. gratissimum was shown to be a Cd accumulator more than a Zn accumulator. Cadmium concentration in its shoots exceeded 100 mg kg?1. In the pot experiments, soils from a heavily Cd-contaminated site (Cd 67.9 mg kg?1 and Zn 2,886.8 mg kg?1) were treated with cow manure and HA at the rates of 10% and 20% (w/w), and 0.75 and 1.5% (w/w), respectively. Plants were grown in the greenhouse for 3 months. The addition of cow manure resulted in the highest biomass production and the lowest accumulations of Cd in plant parts, while HA was more efficient than cow manure in reducing Zn uptake. Leaves of African basil showed a decreased Cd concentration from 1.5 to 0.3 mg kg?1 (cow manure) and decreased Zn concentration from 69.3 to 34 mg kg?1 (HA). This clearly demonstrates the efficiency of HA and cow manure in reducing metal content in leaves of plants grown on high metal-contaminated soil to acceptable or close to acceptable values (0.2 mg kg?1 for Cd, 99.4 mg kg?1 for Zn).  相似文献   

10.

Purpose

Inorganic contaminants present a major challenge for the restoration of aquatic ecosystems. The objectives of this study were to determine the extent of trace metal contamination and investigate the influence of different plant communities on trace metal accumulation in the soils of the Florida Everglades.

Materials and methods

Soil samples (n?=?117) were collected from 0 to 10-cm depth using a stainless steel coring device from sites with three dominant plant communities—cattail, sawgrass, and slough—of Water Conservation Area-2A (43,281 ha) of Florida Everglades.

Results and discussion

The mean pH in soils collected from three plant communities was 6.75–6.82, whereas electrical conductivity was slightly greater in the sawgrass (0.69 dS m?1) than cattail (0.58 dS m?1) and slough (0.40 dS m?1). Mean reduction–oxidation potential was greatest in cattail (?113 mV) than sawgrass (?85.3 mV) and slough (?48.3 mV) soils. Among 11 trace metals (As, B, Co, Cr, Cu, Mn, Mo, Na, Ni, Pb, Zn) found in soil samples, Na had the greatest contents and was greater in cattail (2070 mg kg?1) and sawgrass (1735 mg kg?1) than slough (1297 mg kg?1). Four trace metals (B, Cu, Mo, Ni) were significantly greater in cattail than sawgrass and slough. Whereas, Mn was significantly lower in cattail (31 mg kg?1) than both sawgrass (84 mg kg?1) and slough (51 mg kg?1). Cattail also had significantly lower Cr (1.97 mg kg?1) and Pb (10 mg kg?1) than sawgrass (Cr 2.5 mg kg?1; Pb 20.8 mg kg?1). As (<6.9 mg kg?1), Co (<1.3 mg kg?1), and Zn (<17.2 mg kg?1) were not significantly different among soils collected from three plant community-dominant sites. Contents of Cd and Se were below the method detection limits (Cd 0.01 mg L?1; Se 0.2 mg L?1) and are not reported.

Conclusions

None of the trace metals in the soils exceeded the US Environmental Protection Agency sediment toxicity thresholds. Results from this study provided baseline concentrations of trace metals, which can be used to measure the success of restoration efforts in Florida Everglades.
  相似文献   

11.
A pot experiment was conducted to investigate the effect of chromium compost (0, 10, 30, and 50%) on the growth and the concentrations of some trace elements in lettuce (Lactuca sativa L.) and in the amended soils. Compost addition to the soil (up to 30%) increased dry matter yield (DMY); more than 30% decreased DMY slightly. The application of compost increased soil pH; nitric acid (HNO3)–extractable copper (Cu), chromium (Cr), lead (Pb), and zinc (Zn); and diethylenetriaminepentaacetic acid (DTPA)–, Mehlich 3 (M3)–, and ammonium acetate (AAc)–extractable soil Cr and Zn. The addition of Cr compost to the soil increased tissue Cr and Zn but did not alter tissue cadmium (Cd), Cu, iron (Fe), manganese (Mn), nickel (Ni), and Pb. The Cr content in the lettuce tissue reached 5.6 mg kg?1 in the 50% compost (326 mg kg?1) treatment, which is less than the toxic level in plants. Our results imply that compost with high Cr could be used safely as a soil conditioner to agricultural crops.  相似文献   

12.
Phospho-compost (PC) and poultry manure (PM) were evaluated in field experiments to diversify integrated nutrient management (INM) for rain-fed cotton. Seed cotton yield in the PC (2501–2579 kg ha?1) was similar to the recommended INM (2673 kg ha?1) treatment and was significantly better than nitrogen, phosphorus and potassium (100% NPK) (2130 kg ha?1) and farmers practice (FP) (1886 kg ha?1). Yield was lower in the PM (2476–2617 kg ha?1) than in the PC. Nutrient uptake was higher in all INM intervention plots due to an improvement in soil nutrient status compared with those receiving 100% NPK. Soil labile carbon values were higher in the INM treatments (333–452 mg kg?1), with a greater magnitude in the PC-amended plots (402–452 mg kg?1). Carbon management index (CMI) values were higher for the INM than treatments NPK and FP. Among INM interventions, PC plots had higher values than the PM.  相似文献   

13.
Abstract

Zinc (Zn) deficiency is a widespread micronutrient disorder in crops grown in calcareous soils; therefore, we conducted a nutrient indexing of farmer‐grown rainfed wheat (Triticum aestivum, cv. Pak‐81) in 1.82 Mha Potohar plateau of Pakistan by sampling up to 30 cm tall whole shoots and associated soils. The crop was Zn deficient in more than 80% of the sampled fields, and a good agreement existed between plant Zn concentration and surface soil AB‐DTPA Zn content (r=0.52; p≤0.01). Contour maps of the sampled areas, prepared by geostatistical analysis techniques and computer graphics, delineated areas of Zn deficiency and, thus, would help focus future research and development. In two field experiments on rainfed wheat grown in alkaline Zn‐deficient Typic Haplustalfs (AB‐DTPA Zn, 0.49–0.52 mg kg?1), soil‐applied Zn increased grain yield up to 12% over control. Fertilizer requirement for near‐maximum wheat grain yield was 2.0 kg Zn ha?1, with a VCR of 4∶1. Zinc content in mature grain was a good indicator of soil Zn availability status, and plant tissue critical Zn concentration ranges appear to be 16–20 mg kg?1 in young whole shoots, 12–16 mg kg?1 in flag leaves, and 20–24 mg Zn kg?1 in mature grains.  相似文献   

14.
Removal of soil phosphorus (P) in crop harvest is a remediation option for soils high in P. This 4-year field-plot study determined P uptake by annual ryegrass (ARG, Lolium multiflorum Lam.) and common bermudagrass (CB, Cynodon dactylon (L.) Pers.) from Ruston soil (fine-loamy, siliceous, thermic Typic Paleudult) enriched in P by five previous annual applications of poultry litter, and related P removed to Bray 2 P in surface (0–15 cm) soil. Decreases in surface soil Bray 2 P were largely attributable to uptake. Phosphorus uptake was positively related to Bray 2 P but approached a limit. Mass of P removed in harvest closely approximated the decrease in mass of surface soil Bray 2 P. Maximum Bray 2 P drawdown per harvest (ARG and CB, average) was ?3 mg kg?1 at Bray 2 P ? 300 mg kg?1, generally consistent with measured decreases in Bray 2 P.  相似文献   

15.
Urban horticulture is gaining more and more attention in the context of sustainable food supply. Yet, cities are exposed to (former) industrial activities and traffic, responsible for emission of contaminants. Trace elements were monitored in soils located in the urban environment of Ghent (Belgium) and 84 samples of Lactuca satica L. lettuce grown on it. The effects of cultivation in soil versus trays, neighbouring traffic and washing of the lettuce before consumption were studied. The 0–30 cm top layer of soils appeared heterogenic in composition and enriched in Co, Cd, Ni and Pb within 10 m from the nearest road. Yet, no similar elevated concentrations could be found in the crops, except for As. Besides uptake from the roots, the presence of trace elements in the plants is also caused by the atmospheric deposition of airborne particulate matter on the leaf surface. Correlation analysis and principal component analysis (PCA) revealed that this latter transport pathway might particularly be the case for Pt, Pd and Rh. Concentrations of Cd did not exceed the 0.2 mg kg?1 (fresh weight) threshold for Cd in leafy vegetables set by the European Commission. Measurements to reduce the health risks include the washing of lettuce, which effectively reduced the number of samples trespassing the maximum Pb level of 0.3 mg kg?1 (fresh weight). Also, cultivation in trays resulted in a lower As content in the plants. Taking into account a vigilance on crop selection, cultivation substrate and proper washing before consumption are considered essential steps for safe domestic horticulture in urban environments.  相似文献   

16.
Abstract

Phytotoxicity, due to chromium [Cr (VI)] additions from low to very high levels in a swell–shrink clayey soil (Haplustert), in maize and spinach was studied in a pot culture experiment. Six levels of Cr (VI) (0, 5, 10, 25, 50, and 75 mg kg?1 soil) for maize and five levels for spinach (0, 2, 5, 10, and 25 mg kg?1 soil) were applied singly and in combination with two doses (0 and 20 t ha?1) of city compost. At levels of more than 75 mg Cr (VI) kg?1 soil for maize there was virtually no growth after germination, whereas 25 mg Cr (VI) kg?1 soil hindered the germination of spinach crop. Initial symptoms of Cr (VI) toxicity appeared as severe wilting of the tops of treated plants. Maize plants suffering from severe Cr (VI) toxicity had smaller roots and narrow brownish red leaves covered with small necrotic spots. In spinach, severe chlorosis was observed in leaves. Higher levels of Cr (VI) inhibited the growth and dry‐matter yield of the crops. However, application of city compost alleviated the toxic effect of Cr (VI). The concentration of Cr (VI) in plant parts increased when Cr (VI) was applied singly but decreased considerably when used in combination with city compost. There was evidence of an antagonistic effect of Cr (VI) on other heavy‐metal (Mn, Cu, Zn, and Fe) concentrations in plant tops. Thus, when Cr (VI) concentration increases, the concentration of other beneficial metals decreases. Chromium (VI) concentration in maize roots ranged from traces (control) to 30 mg kg?1and were directly related to soil Cr (VI) concentration. At 25 mg Cr (VI) kg?1 soil, yield of maize was reduced to 41% of control plants, whereas in spinach, 10 mg Cr (VI) kg?1 soil caused a 33% yield reduction. Experimental results revealed that the maize top (cereal) is less effective in accumulating Cr (VI) than spinach (leafy vegetables). Laboratory studies were also conducted to know Cr (VI) sorption capacity of a swell–shrink clayey soil with and without city compost, and it was found that Cr (VI) sorption reaction was endothermic and spontaneous in nature.  相似文献   

17.
Cover crops improve soil quality properties and thus land productivity. We compared soil chemical and biological changes influenced by hairy vetch (Vicia villosa Roth.) and cereal rye (Secale cereal) cover crops grown in Menfro silt loam (fine-silty, mixed, superactive, mesic Typic Hapludalfs), Mexico silt loam (fine, smectitic, mesic Vertic Epiaqualfs), or sand in the greenhouse. Cover crop biomass, soil β-glucosidase, β-glucosaminidase, and fluorescein diacetate (FDA) hydrolase activities, and soil chemical properties were measured at six, nine, and twelve weeks after planting. Cover crop biomass increased with highest (p < 0.0001) yields for hairy vetch and cereal rye in Menfro and Mexico soils, respectively. β-glucosaminidase, FDA, organic carbon (C), total nitrogen (N), and total phosphorus (P) contents significantly decreased in all soils for both cover crops. However, β-glucosidase activity significantly increased (p < 0.0001). Long-term field studies are needed to evaluate soil quality improvement under cover crops, especially for soils with marginal organic matter and fertility.  相似文献   

18.
This study evaluated the petiole uptake of nitrogen, phosphorus, potassium, and sulfur (N, P, K, and S) by the potato from two seed meals, mint compost, and five commercially available organic fertilizers under an irrigated certified organic production system. Available soil nitrate (NO3-N) and ammonium (NH4-N) from each amendment averaged 115 kg N ha?1 at application and 25 kg N ha?1 30 d after planting through harvest, with minor differences between fertilizers. Petiole N declined from an average of 25,000 mg N kg?1, 4 wk after emergence to 3,000 mg N kg?1 prior to harvest. Petiole P and K concentrations were maintained above 4,000 mg P kg?1, 10,000 mg K kg?1, and 2,000 mg S kg?1 tissue, respectively, throughout the growing season in all treatments. Tuber yields were not different between fertilized treatments averaging 53 Mg ha?1. This study provides organic potato growers baseline information on the performance of a diverse array of organic fertilizers and amendments.  相似文献   

19.
Abstract

The low‐hilly regions in South China are mainly covered with Ultisols and Alfisols with a pH of 4.5–6.0. The major factor limiting crop performance is soluble aluminum (Al) in acid soils, resulting in phytotoxicity in susceptible species. An investigation in Jiangxi and Zhejiang provinces in South China showed that many plants including some native plants and cultivated crops (total of 27 species) could grow well in acid soils of these areas. The Al‐accumulating capacity in leaves varied greatly from species to species. Camellia oleifera Abel accumulated more than 13,500 mg kg?1 Al in old leaves; Camellia sinensis (L.) O. Kuntze, Fagopyrum esculentum Moench, and Dicranopteris pedata (Houtt.) Nakaike accumulated more than 1000 mg kg?1 Al in leaves; and Oryza sativa L., Eucalyptus globulus Labill., Citrus reticulata Blanco, and Brassica chinensis L. accumulated less than 200 mg kg?1 Al in leaves. This investigation provides an important basis for further exploring Al accumulation and resistant mechanisms in plants.  相似文献   

20.
The establishment of a complementary grass cover on vineyard soils can promote sustainability of the affected environment. In this work, we used an acid vineyard soil with total Cu concentration 188 mg kg?1 to study the influence of pine bark amendment on Lolium perenne growth and Cu uptake. The results indicate that the pine bark amendment did not cause a significant increase in the mass of the shoots of Lolium perenne, but favored the root biomass: 0.034 g for control and 0.061 g for soil samples amended with 48 g kg?1 of pine bark. Moreover, the pine bark amendment decreased Cu concentration in both, shoots (50 mg kg?1 for control soil and 29 mg kg?1 for soil amended with 48 g kg?1 pine bark) and roots (250 mg kg?1 for control soil and 64 mg kg?1 for soil amended with 48 g kg?1 pine bark). The main factor responsible for these results was a significant decrease of the most mobile fractions of Cu in the soil. Those fractions were extracted using ammonium acetate, ammonium chloride, sodium salt of ethylene-diamine-tetraacetic acid (EDTA-Na), and diethylene-triamine-pentaacetic acid (DTPA).  相似文献   

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