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用0、10、20、30、40、50、60 mg/L浓度的氯化胆碱(CC)溶液分别喷施马蹄金幼苗,研究幼苗相关生理指标的动态变化.结果表明:随着处理浓度的增加,幼苗叶片中的还原糖、可溶性蛋白、叶绿素以及游离脯氨酸的含量均表现先升高后降低的趋势,但还原糖、可溶性蛋白的含量以40 mg/L时的最高,叶绿素和游离脯氨酸的含量则在50 mg/L时最高;丙二醛的含量呈现先降低后升高的态势,50 mg/L的CC时含量最低;超氧化物歧化酶(SOD),过氧化物酶(POD),过氧化氢酶(CAT)的活性在40 mg/L时上升到最高,随后逐渐降低. 相似文献
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硝酸钙处理对菊花扦插生根及抗氧化酶活性的影响 总被引：2，自引：0，他引：2
以切花菊品种‘万盛’为材料,研究了不同浓度Ca(NO3)2处理对菊花扦插生根及其过程中插穗叶片超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性以及膜质过氧化产物丙二醛（MDA）含量的影响。结果表明,60 mg·L-1 Ca2+处理的生根数最多,而且插穗鲜样质量和干样质量也比对照明显增加,而80 mg·L-1Ca2+处理的生根数、插穗鲜样质量和干样质量以及生根率比对照减少。60 mg·L-1 Ca2+处理的SOD、POD、CAT和APX活性与其它处理相比显著增加,MDA含量明显减少。以上结果表明,在菊花扦插过程中,采用适当浓度的Ca2+处理可以提高插穗叶片SOD、POD、CAT和APX等保护酶的活性和降低MDA含量,从而促进菊花扦插生根,并提高扦插苗品质。 相似文献
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《园艺学报》2019,(6)
为了认识硼对菊花生理生化过程的影响,进行了土壤施硼0、0.2、0.5、1.0、2.5、5.0和10.0mg·kg~(-1)的盆栽菊花试验,测定了菊花生长期间叶片的光合色素、可溶性糖、维生素C、脯氨酸(Pro)、丙二醛(MDA)含量和过氧化氢酶(CAT)活性。结果表明,适当的施硼有益于菊花开花和株高增加,但施硼超过2.5 mg·kg~(-1)时,菊花开花延迟。施硼2.5 mg·kg~(-1)处理显著增加了菊花叶片叶绿素的含量,维持稳定的Chl.a/Chl.b和类胡萝卜素含量。土壤施硼0.2～10.0 mg·kg~(-1)增加了菊花植株旺长期叶片可溶性糖含量;施硼0.2～5.0 mg·kg~(-1)处理降低了菊花开花期叶片可溶性糖含量,而施硼10 mg·kg~(-1)处理却使其增加。施硼0.2和0.5 mg·kg~(-1)处理增加了菊花旺长期叶片CAT的活性,5.0和10.0 mg·kg~(-1)处理则使其降低;而施硼0.2～10.0 mg·kg~(-1)均显著增加了开花期叶片CAT的活性。施硼0.5～2.5 mg·kg~(-1)显著增加了开花期叶片的维生素C含量,而施硼5.0和10.0 mg·kg~(-1)则显著降低维生素C的含量。土壤施硼使开花期菊花叶片脯氨酸的含量降低了7.9%～75.5%,施硼量越高,降幅越大。施硼0.2～2.5 mg·kg~(-1)处理对开花期菊花叶片MDA含量无显著影响,而施硼5.0和10.0 mg·kg~(-1)则显著增加了MDA的含量。本试验中适宜的土壤施硼量应不超过2.5 mg·kg~(-1)。 相似文献
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外源水杨酸对盐胁迫下番茄幼苗生理特性的影响 总被引：1，自引：0，他引：1
以盐敏感型番茄“田园六号”为试材,采用水培法,研究了培养液中不同浓度外源水杨酸(SA)(10、50、150、300 mg/L)在100 mg/L NaCl胁迫下,对番茄幼苗超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、根系活力、可溶性蛋白含量、可溶性糖含量以及叶绿素含量的影响.结果表明:盐胁迫增加了SOD活性,提高了MDA、可溶性蛋白和可溶性糖的含量,降低了叶绿素含量和根系活力.50 mg/L SA处理后,MDA含量较只有NaCl处理的降低21.35％,而SOD活性、可溶性蛋白和可溶性糖含量则分别提高了14.15％、74.30％和33.71％,并有效缓解了叶绿素含量的降低和根系活力的下降,其缓解程度高达26.88％和82.03％. 相似文献
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糖为植物生长发育提供能量,也是形态建成的物质基础。但培养基的蔗糖浓度对葡萄试管苗生长及光合作用的影响研究较少,以‘黑比诺’葡萄试管苗为试材,以GS为基本培养基、蔗糖浓度30g/L为对照,分别研究蔗糖浓度0、60g/L对‘黑比诺’葡萄试管苗生理指标和光合参数的影响。结果表明:蔗糖浓度为30g/L试管苗生长状况最佳,试管苗干鲜重、叶绿素含量、可溶性糖含量均显著高于其他2个处理,总糖含量无显著差异;蔗糖浓度60 g/L处理试管苗叶片可溶性蛋白含量显著高于其他2个处理,在高糖及无糖处理下,试管苗叶片蒸腾速率(E)、气孔导度(Gs)与净光合速率(Pn)均显著低于正常浓度处理;叶绿素a、叶绿素b、光合电子传递速率(ETR)、最大量子产量(Fv/Fm)在蔗糖浓度30 g/L处理下均显著高于其他2个处理,叶片中不同激素受蔗糖浓度的影响各不相同。综上,蔗糖浓度能够影响葡萄试管苗生物量、叶绿素相对含量、可溶性蛋白、可溶性糖及总糖含量变化,培养基蔗糖浓度过高或过低会影响叶绿素荧光参数的变化,进而影响光合作用。 相似文献
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SA+CaCl2对菊花花瓣生理和花期的影响 总被引：1，自引：0，他引：1
以不同浓度的水杨酸(SA+CaCl2)水溶液对菊花"兼六香菊"品种全株喷雾处理,测定整个花期花瓣中可溶性蛋白、可溶性糖含量、超氧化物歧化酶(SOD)活力、超氧阴离子(O2-)产生速率、丙二醛(MDA)含量以及相对电导率的变化.结果表明:不同浓度的SA+CaCl2均能提高花瓣中可溶性蛋白和可溶性糖含量、SOD活力和降低了O2-产生速率、MDA含量和相对电导率,花期有不同程度的延长,其中以SA 15 mg/L+CaC12160 mg/L处理效果较佳. 相似文献
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不同植物生长调节剂及浓度对曼地亚红豆杉扦插生根的影响 总被引：2，自引：0，他引：2
以曼地亚红豆杉1年生半木质化嫩枝插穗为试材,研究了4种不同植物生长调节剂(IBA、ABT1、NAA、IAA)和4种不同浓度处理(100mg/L浸泡1h、300mg/L浸泡0.5h、500mg/L速蘸5s和700mg/L速蘸5s)对曼地亚红豆杉扦插生根的影响。结果表明:NAA处理时,成活指标以700mg/L NAA速蘸5s处理效果最好,生长指标以100mg/L NAA浸泡1h处理效果最好;IAA处理时,成活指标以300mg/L IAA浸泡0.5h为最佳,生长指标以500mg/L IAA速蘸5s为最优;ABT1处理时,100mg/L ABT1浸泡1h处理生根效果最好;IBA处理时,300mg/L IBA浸泡0.5h处理生根效果最好,700mg/L速蘸5s时平均梢长最长;不同植物生长调节剂种类和浓度处理会不同程度的影响曼地亚红豆杉扦插生根的效果,不同生长调节剂种类对扦插生根效果影响显著,浓度处理对生根效果无显著影响。 相似文献
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The effects of combinations of salinity (no salt, 2000 p.p.m. or 4000 p.p.m. of CaCl2 and NaCl, 1:1) and water table (30 cm, 60 cm or 90 cm from the soil surface), on the vegetative growth and tolerance of ‘Golden Japanese’ plum and ‘Mit Ghamre’ and ‘Balady’ peaches were studied. The plants were grown in lysimeters. The growth of the trunk, total shoot length, the increase in shoot length per cm and the fresh weight of top, root and total plant were reduced with increasing salinity of the irrigation water. The effect was accentuated when the plants were maintained at high water table level. The salinity treatments resulted in the death of 43%, 73% and 76% of the plants in the plum, and the ‘Mit Ghamre’ and ‘Balady’ peaches, respectively, indicating that the plum is more tolerant to salinity than the peach. The plants of the salinity treatments showed various symptoms of salt injury, such as leaf burn, defoliation, shoot die-back and finally death. In the peaches, salt injury started to occur in the first growing-season, whereas the salt injury appeared in the plum in the second growing-season. The symptoms were more pronounced in the 4000 p.p.m. treatment than in the 2000 p.p.m. treatment and were more pronounced at the high water table level. The salinity level was the predominant factor and the effect of the water table on the vegetative growth diminished with increase in the salinity level of the irrigation water. 相似文献
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AIM：To establish a fast, accurate and economical technique for culturing mouse pulmonary arteriolar smooth muscle cells (PASMCs), and to explore the effects of hypoxia on the proliferation and apoptosis of the PASMCs. METHODS：In sterile condition, the pulmonary artery was isolated from the male BALB/c mice by digesting with collagenase I, and the cells were cultured in fetal bovine serum-coated flask. Centrifugal procedure was not used during the cell passage. The cell morphology was observed under an inverted phase-contrast microscope. α-Smooth muscle actin was identified by immunocytochemistry and immunofluorescence. The effects of hypoxia on the proliferation and apoptosis of the PASMCs were detected by CCK-8 assay and TUNEL assay. RESULTS：PASMCs were identified by the methods of immunocytochemistry, immunofluorescence staining and observation of morphology. Unlike the rat PASMCs with typical subcultured peak-vally pattern, the mouse PASMCs showed a lot different without a peak-vally pattern. The cells could be subcultured after 5 d to 7 d and there was 3 to 5 generations depending on the activity of the cells. CCK-8 assay demonstrated that the A values of PASMCs exposed to hypoxia increased after 24 h (P＜0.05) as compared with normoxia. TUNEL result showed that the apoptotic index of the PASMCs in hypoxia decreased after 24 h (P<0.05). CONCLUSION：This technique for obtaining cultured mouse PASMCs is simple, fast, accurate and economical. The digestion time is easy to control. Hypoxia promotes the proliferation and inhibits the apoptosis of PASMCs. 相似文献
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小檗碱和壳聚糖抗蔬菜病原真菌活性测定及复合膜制备 总被引：2，自引：0，他引：2
研究了小檗碱和壳聚糖对几种常见蔬菜病原真菌的抑制活性,以及以小檗碱和壳聚糖为主料制备复合膜的方法,并测定了该膜的药物释放效果。试验表明:低浓度(0.234 mg/mL)小檗碱即可抑制辣椒炭疽病菌(Vermicularia capsici)等5种蔬菜病原真菌的生长。20 mg/mL浓度壳聚糖对番茄灰霉病菌(Botrytis cinerea)的抑制率高达65%,而对其余4种果蔬病原真菌也有一定的抑制作用。为了集成这2种天然化合物的优点,制备了小檗碱-壳聚糖复合膜,该膜具有缓释功能,在模拟外部环境(磷酸缓冲液,pH 6.8)条件下,20 d小檗碱累计释放率接近25%,提示其在果蔬贮藏抗菌中的应用价值。 相似文献
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Fresh onion seeds desiccated to 6.0% seed moisture content (SMC) were stored in various packaging materials under different storage conditions. Seeds packed in aluminum-laminated pouches beside those stored with silica gel at 25 °C maintained satisfactory germinability and vigour after 12 months. Desiccated seeds stored in moisture impervious containers produced more vigourous seedlings. Germination potential of onion seeds increased with reduced SMC besides storage in moisture impervious packets along with desiccants as physiological and biochemical attributes are regulated. Seed viability and vigour decreased with accelerated ageing due to increased lipid peroxidation, decreased activities of several free radical and peroxide scavenging enzymes. Electrical conductance of seed leachates also increased with ageing. Thus, adoption of appropriate storage temperature and moisture control techniques would significantly affect onion seed quality, which was due to minimum accumulation of free peroxide radicals and enhanced activity of free radical scavenging enzymes. 相似文献
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以大荔县冬枣种植区采集的发病枣果为试材,采用常规组织分离法进行病原菌的分离纯化,通过柯赫氏证病法则、形态学观察及分子生物学分析,研究鉴定了冬枣黑斑病和炭疽病的病原菌种类,并采用田间调查法对2种病害为害特征进行比较分析,以期为田间有效区分冬枣黑斑病和炭疽病为害症状,并快速判断其病原类型,及进一步深入开展精准防控提供参考和指导.结果表明:陕西大荔冬枣黑斑病的病原菌为细极链格孢(Alternaria tenuissima),炭疽病的病原菌为胶孢炭疽菌(Colletotrichum gloeosporioides).2种病害均主要为害枣果实,在枣果果腰或果肩上形成黑褐色病斑,可能同时存在且症状相近极易混淆,但在病斑表面、皮下及久置表型3个方面存在明显区别. 相似文献
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钾肥在小白菜和萝卜上的施用效果 总被引：6，自引：0，他引：6
采用盆栽试验方法研究了施用钾肥对小白菜、萝卜生长发育、产量、品质及钾素含量的影响。结果表明 ,施用钾肥对小白菜和水萝卜的株高、叶片数无显著的影响 ,但可提高水萝卜块根的产量 ,提高幅度为 31.0 4 %～ 97.93%。施用钾肥可提高小白菜和萝卜的蛋白质和VC含量 ,降低硝酸盐含量 ,增加植株的钾素含量 相似文献