共查询到19条相似文献，搜索用时 218 毫秒
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采用盐酸萘乙二胺法测定了西宁市猪肉熟制品中亚硝酸盐的残留量。结果表明：在检测的48份样品中有45份阳性，亚硝酸盐的平均残留是14.87mg/kg，最高量达89.9mg/kg。 相似文献
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消毒是采用物理学、化学、生物学手段杀灭和减少生产环境中病原体的一项重要技术措施。其目的在于切断疫病的传播途径,防止传染性疾病的发生与流行,是综合性防疫措施中最常采用的重要措施之一。笔者在近五年的种猪生产与实践中总结了几点,与广大同行共同交流。 1 猪场消毒制度的建立 1．1 进入猪场大门前应设置消毒池,水深10～15cm,配制成2%氢氧化钠溶液。 1．2 猪场消毒室设置紫外线灯管,进场前消毒5～10min。 1．3 工作栋设置小消毒池,配成2%的氢氧化钠溶液。 1．4 工作人员进场前必须更换工作服、鞋,有条件可以洗澡。 1．5 猪群年龄结构不同,饲养工作人员严禁串栋。 1．6 猪舍每天打扫2～3次,料槽水槽干净并无饲料结块。 1．7 栏舍、场内空地应定期消毒。 2 全进全出制生产,空猪舍的常规消毒程序 2．1 首先对空栏应彻底清扫干净。 2．2 用2%的氢氧化钠溶液喷洒,然后刷洗干净,经1～2h后,再用清水冲洗。 2．3 福尔马林薰蒸消毒,37%～42%的甲醛溶液按每立方米空间15～20ml,关闭门窗,用火加热,经1h即可。 3 消毒时注意事项 3．1 大门、小门入口处消毒池应7～15d更换一次消毒液。 3．2 畜舍空栏洗后消毒应在完全干燥后进行。 3．3 消毒剂应广谱,多种类消毒剂交叉使用,稀释浓度应正确。 3．4 消毒时防止药物腐蚀猪皮肤和猪中毒等。 作者电话:0724-7224987 传真:0724-7221942 相似文献
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为探究RT-PCR技术在口蹄疫病毒中的应用，采集4家养殖场不同猪、牛、羊群血清样本共300份，检测口蹄疫O/GX/09-7株，按照病料处理、标准品RNA制备、DNA的聚合酶链式扩增、回收纯化、感受态细胞制备、阳性菌液保存步骤，以仪器(试剂)默认值为参考，判断检测结果。检测结果表明猪阳性率病例数为2例，阳性病例占比为1.92%(2/104),牛羊阳性率较低，各为1例，阳性病例占比分别为0.98%(1/102)、1.06%(1/94),总检出率1.33%(4/300)。相关部门和技术人员应根据口蹄疫检测结果，依据阳性病例数量、日龄、高发时段、高发动物类型等，第一时间做好消毒、扑杀、净化等一系列处理工作，以进一步控制疫情蔓延。 相似文献
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应用常规细菌分离法对西宁市某几个农贸市场采集的70份仔虾样品进行了副溶血性弧菌的检测,经细菌形态观察、嗜盐性试验、细胞色素氧化酶试验和生化特性测定,鉴定为副溶血性弧菌。结果检出阳性菌株4株,阳性率为5.7%(4/70),同时通过致病性试验证明4株分离菌对小白鼠有致死效应。 相似文献
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Mustafa Yipel Cemil Kürekci İbrahim O Tekeli Murat Metli Fatih Sakin 《Aquaculture Research》2017,48(7):3829-3836
The aim of the study was to monitor the occurrence of 37 antibiotics from different classes including fluoroquinolone, tetracycline, macrolide, sulphonamide, penicillin, amphenicol and cephalosporin in three different farmed fish species (n = 75; rainbow trout, see bass, gilthead see bream) from five cities in Turkey. A method for LC‐MS/MS was optimized and validated to assess these antibiotics. As a result, 25 samples showed contamination with at least one antibiotic residue. The most detected antibiotics were norfloxacin (NOR) (18.7%), marbofloxacin (MAR) (12%), tetracycline (TC) (10.7), danofloxacin (DAN) (9.3%), oxytetracycline (OTC) (9.3%), tilmicosin (TLM) (8%) and enrofloxacin (ENR) (6.7%). A total of 17.3% of samples examined were found to be contaminated with residues exceeding the maximum residue limits (MRLs). The results indicate that antibiotics are frequently used in aquaculture production without the lack of knowledge. This study also revealed that the consumption of farmed fish could produce a public health concern as the level of residues for some antibiotics was much higher than MRLs. 相似文献
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Relative Prevalence of Antibiotic Resistance among Heterotrophic Bacteria from Natural and Culture Environments of Freshwater Prawn,Macrobrachium Rosenbergii 
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下载免费PDF全文 Abdulla A. Mohamed Hatha A. Deborah Gnana Selvam Ambatt P Thomas 《Journal of the World Aquaculture Society》2016,47(4):470-480
As antibiotic residues and multidrug‐resistant (MDR) bacteria are of increasing concern to those involved in the seafood industry, there is an intense international focus to monitor MDR bacteria in seafood. There is also a trend to source seafood from organically raised farms in order to reduce antibiotic usage in culture operations. Hence this study was conducted to compare the antibiotic resistance among bacteria associated with various samples from the natural and culture environment of Macrobrachium rosenbergii. Samples from the natural environment included water, sediment, eggs, larvae, postlarvae (PL), feed items, and gut samples of M. rosenbergii and those from the culture environment were water, sediment, eggs, feed pellets, and gut samples of M. rosenbergii. Bacteria from water samples of natural and culture environments were frequently resistant to ampicillin, erythromycin, and penicillin, while more than 50% of the strains from sediment were resistant to erythromycin, nalidixic acid, and penicillin. Bacterial isolates from commercial feed had a relatively high degree of antibiotic resistance. Interestingly, occurrence of multiple drug resistance was higher among the bacteria associated with the samples from the natural environment of M. rosenbergii, pointing to more favorable selection pressure for drug resistant mutants in the natural environment. 相似文献
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对虾传染性皮下及造血器官坏死病毒(IHHNV)可感染世界各地养殖对虾,给对虾养殖业造成严重经济损失。本实验首次采用实时定量PCR法对广西地区的84份凡纳滨对虾样品进行检测,同时以常规PCR检测作对照。实时定量PCR检测阳性率为79·8%,常规PCR检测阳性率为40·5%,表明广西地区养殖的凡纳滨对虾IHHNV的感染率较高。将二者检测均呈阳性的30份样品扩增产物进行序列分析测序,测序结果通过DNA STAR软件包进行分析,并通过NCBI Blast与GenBank中的序列进行比对。结果证明,测定的是IHHNV序列。30份样品的IHHNV序列很保守,可以分为4种类型,仅有两个碱基的位置发生变异。实时定量PCR检测IHHNV,快速、灵敏、准确,特异性好,可以作为检测对虾感染病毒的有效方法。 相似文献
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Amin Abrahim Daniel Sergelidis Ioannis Kirkoudis Vasiliki Anagnostou Eleni Kaitsa-Tsiopoulou Polyzo Kazila 《Journal Of Aquatic Food Product Technology》2013,22(2):93-102
The presence and antibiotic resistance of Staphylococcus spp. in freshwater fish and the environment of fish markets of Northern Greece were investigated. A total of 269 samples were examined, consisting of 71 rainbow trout (Oncorhynchus mykiss), 65 gibel carp (Carassius gibelio), and 133 environmental samples swabbed from various surfaces at fish markets.Staphylococcus spp. was isolated from 27% of the samples, and 16%, 9%, 0.7%, 0.4% and 0.4% were found to be positive for S. aureus, S. epidermidis, S. warneri, S. hominis-hominis, and S. haemolyticus, respectively. All isolates were examined for their susceptibility to 20 antibiotics. None of the S. aureus isolates were resistant to oxacillin and glycopeptides. Three S. epidermidis, two S. Warneri, and one S. haemolyticus isolates were resistant to oxacillin (methicillin resistant Staphylococcus, MRS). All isolates, except one, showed resistance to as many as 15 antibiotics. The population of Staphylococcus spp. in fish did not exceed 1.0 log CFU/g. Freshwater fish and the environments of retail fish markets were found to harbor multi-drug resistant staphylococci. Whether these findings present a real health risk for humans and to what extent needs to be evaluated. 相似文献
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Prevalence and antimicrobial resistance of vibrios of human health significance in inland saline aquaculture areas 下载免费PDF全文
下载免费PDF全文 Balwinder Singh Anuj Tyagi Naveen K Billekallu Thammegowda Meera D Ansal 《Aquaculture Research》2018,49(6):2166-2174
This study was conducted to evaluate the prevalence, potential pathogenicity and antimicrobial resistance of Vibrio isolates from 65 soil/water/fish samples collected from inland saline aquaculture areas. Depending on the sample type, presumptive Vibrio counts ranged from 2.50 to 6.16 log10 CFU/ml (or/g). Among the 119 confirmed Vibrio isolates, Vibrio cholerae was found to most dominant (91.6%) and it was detected in all the samples from inland saline areas. Seven other Vibrio spp. including Vibrio parahaemolyticus and Vibrio vulnificus were also detected. Except one O139 serotype, rest of the V. cholerae isolates were found belonging to non‐O1/non‐O139 serogroups. None of the V. cholerae isolate was found positive for ctx gene. Antimicrobial susceptibility testing against 7 commonly used antibiotics revealed highest resistance (50.4%) against ampicillin. Very high intermediate resistance (87.4%) was also observed against erythromycin. Contrary to previous studies, high susceptibility (>70%) to chloramphenicol, nalidixic acid, tetracycline and trimethoprim was observed in Vibrio isolates obtained in present study. Almost 20% of Vibrio isolates were resistant to two or more antibiotic classes with multiple antibiotic resistance (MAR) index value of ≥0.28. Presence of V. cholerae isolates with very high MAR index value of 0.85 also suggested that these multidrug‐resistant environment isolates could serve as reservoir of antibiotic‐resistant genes in aquatic systems. The presence of multiple drug resistance vibrios in emerging inland saline aquaculture systems emphasizes the need for their routine monitoring for developing the risk assessment and mitigation strategies. 相似文献
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Abstract. Cultivation plates for the primary isolation of Renibacterium salmoninarum were incubated for 12 or more weeks over 3.5 years. The incubation time for sample of two groups of Atlantic salmon, Salmo solar L., broodfish on a selective agar medium is reported. Samples turned positive over a long time-span. but the longest incubation time recorded for growth of the bacterium was 19 week. In one group, 79% of all positive samples form fish with macroscopic signs of bacterial kidney disease were positive after 6 weeks of incubation and the highest incidence of positive samples occured during the sixth week. From covertly infected fish within the same group.42% of sample were positive after 6 weeks of incubation, and the highest incidence of positive sample occured during the sixth and the ninth weeks of incubation. The growth pattern of the bacterium at primary isolation was characterized by a prolonged lag phase followed by a relatively growth in one week from microscopic to macroscopic size of the colonies. 相似文献
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Abstract. The effectiveness of three different growth media KDM-2, S-KDM and S-KDM-C for primary isolation of Renibacterium salmoninarum was examined over a 14-week incubation period. Kidney samples were taken from Atlantic salmon, Salmo salar L., broodfish returning to a sea ranch after 2 years at sea. Homogenized samples were inoculated onto two selective media, S-KDM supplemented with lamb serum and S-KDM-C supplemented with activated charcoal. The third medium was the non-selective serum supplemented KDM-2. Renibacterium salmoninarum was isolated from 112 samples on one or more of the media used. Of all positive samples, 91% were positive on S-KDM, 60% on S-KDM-C and 35% on KDM-2. These results demonstrate that selectivity significantly enhances the isolation capacity of the medium and that supplementing with serum is significantly more effective than supplementing with charcoal. 相似文献
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胶体金免疫层析法快速检测水产品中呋喃妥因代谢物残留 总被引：1，自引：0，他引：1
采用胶体金免疫层析技术(GICA)测定水产品中的呋喃妥因代谢物残留,并用液相色谱-串联质谱(LC-MS/MS)对实验结果进行确证。结果显示,胶体金免疫层析法的检测限为1.0μg/kg,假阳性率不大于5%,假阴性率为0;对实际样本的检测结果与LC-MS/MS法一致。研究表明,胶体金试纸条法操作简便、快速,成本低,为水产品中呋喃妥因代谢物的残留检测提供了快速筛选方法,而LC-MS/MS法灵敏度高,结果准确,适用于阳性样本的确证和精确定量。 相似文献