共查询到19条相似文献，搜索用时 374 毫秒
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马铃薯脱毒试管苗生产是马铃薯脱毒微型薯产业化的重要环节,但组培苗生产成本较高,严重制约了脱毒种薯产业化的发展。本文以工厂化生产组培苗直接成本与间接成本为线索,从经营管理与技术更新上提出一些既能保证试管苗质量,又能降低生产成本的有效管理措施,对非机械化生产组培室有一定的参考价值。 相似文献
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马铃薯试管苗工厂化生产降低成本研究 总被引：4，自引：1，他引：4
1 前言近年来,马铃薯的脱毒组培生产在我国有了很大发展,取得了很大成绩.但是存在着一个普遍问题是马铃薯试管苗成本偏高,在一定程度上影响了其发展.几年来,我们菜芜市农科所在马铃薯试管苗工厂化生产降低成本上做了一些研究,现将结果报告如下. 相似文献
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1 前 言试管苗的工厂化扩繁生产随着规模的不断扩大 ,逐渐建立了一套高效低成本的试管苗工厂化生产技术本系。在长期的探索中 ,对该体系的生产成本进行了细致、准确的核算 ,目的是不断降低成本 ,提高公司的经济效益和社会效益。2 试管苗的工厂化生产我们采用传统的人工光照组织培养进行马铃薯试管苗的工厂化生产。在此生产技术基础上 ,探索出一些适合当前工厂化生产的管理技术措施。2 1 培养基成分的改进(1)通过试验 ,去掉MS基本培养基中的肌醇及其高激素成分 ,对试管苗的生长影响不明显 ;(2 )用廉价、方便的自来水代替昂贵的纯净水或… 相似文献
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马铃薯脱毒试管苗扦插剪尖次数与繁殖系数及经济收益分析余显蓉，李艳（四川省凉山州西昌农科所西昌６１５０００）１－ｇｉ言在马铃薯脱毒微型薯工厂化生产中，围绕降低脱毒微型薯成本，加快繁殖速度这一问题，我们作了试管苗扦插成活后，在７０ｄ收薯的前提下，不同剪尖... 相似文献
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网室中马铃薯脱毒微型薯生产技术 总被引：1，自引：0，他引：1
在常规的网室马铃薯脱毒微型薯的生产中,脱毒试管苗的移栽成活率在60%左右,甚至更低,对人力、财力是很大浪费。为了解决这个问题,本文从防虫网棚温室的设施、基质的配制、水肥管理、病虫害综合防治措施等方面进行了改进和建立规范化生产,使试管苗移栽成活率达到97%,以期为高效低成本马铃薯脱毒微型薯工厂化生产提供技术参考。 相似文献
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不同培养方式和光照强度对马铃薯脱毒试管苗微繁的影响 总被引：10，自引：0，他引：10
为适应马铃薯种薯生产工厂化的需求,从高效低成本出发,对影响马铃薯脱毒试管苗微繁的培养方式及自然光的有效利用进行了研究。结果表明,液体培养显优于固体培养,而且在普通棉和脱脂棉之间,在上部鲜重,叶片数,有效节数和株高等动态指标上无明显差异,但普通棉却大幅度降低了成本。利用充足的自然光可以满足试管苗正常生长,从而减少用电消耗,降低成本。 相似文献
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Maintaining potato viruses X, S, Y, A, M and leafroll in tissue culture plantlets is a convenient, cost and space effective alternative to the use of greenhouse plants. Of these six viruses, only certain strains of PVX induced symptoms in tissue culture plantlets. Nevertheless, all infected tissue culture plants were found to be more reliable than greenhouse grown plants as virus-infected controls in enzyme-linked immunosorbent assay (ELISA) testing. Another advantage of maintaining viruses in tissue culture plantlets was the elimination of contamination by other viruses or other pathogens. Leaves, stems, and roots of virus infected plantlets were tested separately for antigen levels by ELISA. In these tests, the stems and leaves of all but PVA infected tissue culture plants consistently gave positive ELISA values. In contrast, root tissue from PVY infected tissue culture plantlets was not reliable for PVY detection. In all cases, the viruses detected in the original source material were detected in the resulting tissue culture plantlets. 相似文献
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Katarzyna L. Janczur Mark K. Nakhla Amy O. Charkowski 《American Journal of Potato Research》2006,83(6):495-501
Potato plantlets initiated into tissue culture must be tested for numerous viruses prior to propagation for seed potato production. Ideally, one plantlet is tested for all pathogens of concern and, if found pathogen-free, this plantlet is propagated for production of seed potatoes. Commercially available ELISA kits are generally used for the pathogen tests, but the commercial kits have some limitations. For example, the protocols differ for different viruses, so multiple extractions must be completed, increasing the time and expense of testing. This is a significant problem with tissue culture plantlets, for which there is limited material available to test and an ever-increasing number of pathogens that must be tested for, including viruses in the potyvirus, carlavirus, potexvirus, luteovirus, pomovirus, tobravirus, tospovirus, alfamovirus, and tymovirus groups. We have optimized a non-radioactive nucleic acid hybridization (NASH) assay for the simultaneous detection of carlavirusPotato virus S (PVS), potexvirusPotato virus X (PVX) and potyvirusPotato virus Y (PVY) in potato tissue culture plantlets. This assay requires a single extraction from a small portion of a tissue culture plantlet for the detection of viruses from three different families. 相似文献
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【目的】对一品红组培苗移栽技术进行研究。[方法】对一品红茎段进行组织培养,并以此为材料对一品红炼苗时间、移栽地点、移栽时间和移栽基质进行筛选试验。【结果】一品红组培苗应提前7d以上进行炼苗;移栽的时间阜新地区选择在5月初最佳;移栽地点普通温室和智能温室都可;移栽的基质以草炭＋珍珠岩成活率高,在降低成本的情况下,1／2草炭＋1／2园土也比较适合。[结论】通过移栽时间和基质的选择,能有效提高一品红组培苗的成活率。 相似文献
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用组织培养法繁殖野生亚麻的研究 总被引：8，自引：1，他引：7
野生亚麻是优异的种质资源，由于其种子具有较强的休眠性，用常规方法繁殖难度较大，本试验利用组织培养方法，通过对实生苗幼嫩的茎杆及叶片诱导愈伤组织再分化苗的途径，对野生亚麻的试管苗扩繁进行了研究，获得了一定数量的野生亚麻再生无根苗。研究结果表明，野生亚麻愈伤分化成苗对培养基的选择性不强，用组织培养法快速繁殖是行之有效的途径。 相似文献
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基施有机肥对马铃薯原原种生产及二次结薯的影响 总被引：1，自引：0，他引：1
以早熟马铃薯‘早大白’为供试品种,在防虫温室内进行了有机肥对脱毒马铃薯试管苗生长及二次结薯影响的试验,旨在探索提高微型薯产量及试管苗利用率的途径。试管苗移栽70 d后收获微型薯并尽量保护好根系,继续使其结薯,进行多次收获。结果表明:多次收获微型薯的产量远高于一次性收获的微型薯产量,因此二次结薯可大大提高试管苗的生产效率。全部追施处理二次结薯单株结薯2.3粒,而基施有机肥33 kg/667 m2条件下单株结薯3.8粒,因此基施有机肥有利于试管苗的生长。 相似文献
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Micropropagation is a tissue culture technique adapted for the rapid multiplication of disease-free seed stocks. Procedures for propagating potatoes in the laboratory and acclimating plantlets in the greenhouse are available, but information on cultural practices for maximizing tuber yield of plantlets when transplanted to the field is lacking. Centennial Russet and Russet Burbank plantlets were transferred from culture jars to three sizes of transplant containers for establishment under greenhouse conditions before transplanting to the field. Length of field growing season was varied by using two transplant dates and two vine kill dates. Survival of field transplanted plantlets was above 95 percent in both 1983 and 1984. Cultural practices significantly affected the tuber yield of plantlets of both cultivars. Total yield and yield of tubers larger than 35 mm in diameter increased with increasing transplant container volume. Transplant container volume had no effect on the yield of tubers less than 35 mm in diameter. Highest total yields and tuber production per plantlet for both Centennial Russet and Russet Burbank were obtained from the longest growing season (early transplant date with late vine kill). Yield of Russet Burbank plantlets increased more in response to a longer growing season than did Centennial Russet. 相似文献