共查询到20条相似文献，搜索用时 343 毫秒
1.
试验旨在探讨油橄榄叶提取物（olive leaf extract，OLE）防治酒精性脂肪肝（AFLD）的作用机制。将50只健康大鼠随机均分为5组：空白对照组，模型组，OLE高、中、低剂量组，除空白对照组外，其他各组均采用递增法灌胃食用乙醇24周，建立大鼠肝损伤模型，造模同时OLE高、中、低剂量组分别用OLE（1 000、500、250 mg/kg）进行灌胃治疗。利用生物显微技术观察肝脏组织结构的变化，全自动生化分析仪测定血脂水平，实时荧光定量PCR、Western blotting和酶联免疫吸附法（ELISA）检测肝组织蛋白磷酸酶1（PP1）、DNA依赖的蛋白激酶（DNA-PK）、上游刺激因子1（USF1）mRNA水平和蛋白含量。结果显示，与空白对照组相比，模型组大鼠肝脏损伤程度明显加重，血清甘油三酯（TG）、游离脂肪酸（FFA）含量极显著升高（P<0.01）；肝脏PP1、DNA-PK、USF1水平和蛋白含量极显著升高（P<0.01）。与模型组相比，OLE干预后，肝脏病理改变明显减轻；血清TG、FFA含量显著或极显著降低（P<0.05；P<0.01）；肝脏PP1、DNA-PK、USF1 mRNA水平和蛋白含量显著或极显著降低（P<0.05；P<0.01）。结果表明，OLE可通过调节PP1-DNA-PK-USF1信号通路缓解肝脏脂肪变性，抑制AFLD的发展。 相似文献
2.
文章旨在研究基础日粮添加不同水平黄芩苷对育肥猪生长性能、免疫机能及抗氧化能力的影响。试验将健康状况良好、品种相同、日龄和初始体重相近的100头育肥猪随机分为4组,每组5个重复,每个重复5头。4组育肥猪分别饲喂基础日粮+0、200、400、600?mg/kg黄芩苷的日粮,试验为期45?d。整个试验期间,育肥猪自由饮水、自由采食,且按照规模化养殖要求进行分栏饲养。结果 ：（1）200?mg/kg黄芩苷组育肥猪结测体重（EW）、平均日增重（ADG）、平均日采食量（ADFI）均显著高于0、600?mg/kg黄芩苷组（P <0.05）,200?mg/kg黄芩苷组育肥猪料重比（F/G）显著低于0?mg/kg黄芩苷组（P <0.05）。（2）600?mg/kg黄芩苷组血清中免疫球蛋白A(IgA)、免疫球蛋白G(IgG)含量显著高于0?mg/kg黄芩苷组（P <0.05）,400、600?mg/kg黄芩苷组血清中白细胞介素-6(IL-6)含量显著高于0?mg/kg黄芩苷组（P<0.05）。（3）600?mg/kg黄芩苷组血清中总抗氧化能力（T-AOC）活性显著高于0?mg/kg... 相似文献
3.
本试验旨在研究芦丁对山羊组织脂肪酸合成和代谢相关基因表达的影响。试验选取36只体重相近且健康的努比亚山羊,随机分成3组,每组12只羊。对照组不添加芦丁,两个试验组分别添加25mg/kg、50 mg/kg芦丁,试验期为70d。结果表明：（1）芦丁对山羊肝脏、空肠和肌肉的ACOX1 mRNA表达量无显著影响（P> 0.05）。（2）与对照组相比,50mg/kg组山羊肝脏的PPAR-γ和ACACA mRNA表达量显著升高（P <0.05）,25 mg/kg组山羊空肠的PPAR-γ mRNA表达量显著升高（P <0.05）。对照组山羊肌肉的PPAR-γ和ACACA mRNA表达量显著高于其他两组（P <0.05）。（3）与对照组相比,25 mg/kg和50 mg/kg组山羊肝脏的FASN mRNA表达量显著升高（P <0.05）,而空肠的FASN mRNA表达量则相反。50 mg/kg组山羊肌肉的FASN mRNA表达量显著低于其他两组（P <0.05）。综上所述,芦丁能够通过调控脂肪酸合成和代谢相关基因的表达来影响山羊体内脂肪的沉积。 相似文献
4.
试验选取48只21日龄断奶SD大鼠,随机分成4个处理,试验组分别灌胃小檗碱(30、120mg/kg体重)、黄连水提物(含9.9%的小檗碱,300mg/kg体重),另设空白对照组。试验共15d。结果表明:30mg/kg体重小檗碱和300mg/kg体重黄连水提物均显著提高大鼠平均日增重(P<0.05),降低粪中大肠杆菌数量(P<0.05),增加回肠绒毛高度和绒毛高度/隐窝深度(P<0.05);300mg/kg体重黄连水提物还显著提高了大鼠胸腺和脾脏指数(P<0.05);120mg/kg体重小檗碱显著降低粪中大肠杆菌数量(P<0.05),提高大鼠胸腺和脾脏指数(P<0.05),增加回肠绒毛高度(P<0.05),但平均日增重与空白对照组相比无显著差异(P>0.05)。综上所述,300mg/kg体重黄连水提物在促进大鼠生长发育方面效果最佳。 相似文献
5.
本试验旨在建立阿司匹林诱导的大鼠肠道损伤模型。试验采用单因素试验设计,选用6周龄SD大鼠18只,经过7 d的适应性饲养后随机分为3个组,每组6只,单笼饲喂。模型1组和模型2组阿司匹林的灌胃剂量分别为50和200 mg/kg,空白对照组灌胃等量生理盐水,持续灌胃14 d。结果表明:与空白对照组相比,灌服50 mg/kg阿司匹林显著降低了大鼠的体增重、血清白细胞介素-2(IL⁃2)含量、肠道黏膜分泌性免疫球蛋白A(sIgA)含量、肠道绒毛高度和绒毛高度与隐窝深度的比值(P<0.05),但对肝脏指数、脾脏指数和血清溶菌酶(LZM)活性没有显著影响(P>0.05);灌服200 mg/kg阿司匹林显著降低了大鼠的体增重、肝脏指数、血清IL⁃2含量与LZM活性、肠道黏膜sIgA含量、肠道绒毛高度和隐窝深度及二者的比值(P<0.05)。在本试验条件下,采用200 mg/kg剂量的阿司匹林连续灌胃14 d可以成功建立大鼠的肠道损伤模型。 相似文献
6.
目的:探讨黄芪复方剂提取物总黄酮(EFA)对CCl4致大鼠肝氧化损伤的保护作用及机制。方法:将雄性SD大鼠60只随机分成4组,即灌胃灭菌生理盐水空白对照组和模型组、联苯双酯(100 mg/kg)溶液阳性药物对照组、黄芪复方剂提取物总黄酮(200 mg/kg)组。除空白对照组腹腔注射等量生理盐水外,其余各组腹腔注射四氯化碳玉米油(2 mL/kg)造成大鼠肝氧化损伤模型,后分别于12、24 h将相应大鼠处死,检测血清中ALT、AST、ALB含量与肝组织中MDA、SOD、GSH-PX活性。结果:与模型组比较,经黄酮药物组预防性治疗后,AST、ALT转氨酶明显降低(P<0.05),而ALB含量增加不明显;同时肝脏中MDA含量明显降低(P<0.05),SOD、GSH-PX活性接近空白组,且明显高于模型组(P<0.01)。结论:EFA对四氯化碳造成的大鼠肝氧化损伤具有保护作用。 相似文献
7.
那西肽对猪生长性能的影响 总被引：5，自引：0，他引：5
选用日龄、体重相近,发育正常的杜×长×大三元杂交健康仔猪170头,随机分成5组,空白对照组、2.5 mg/kg那西肽组、5 mg/kg那西肽组、10 mg/kg那西肽组和20 mg/kg维吉尼亚霉素组。每组设3个重复栏,每个重复栏11～12头猪。饲喂28 d,结果显示,日粮中添加了抗生素的试验组较空白对照组在日增重上均有提高,2.5、5、10 mg/kg那西肽组的日增重分别比空白对照组提高了10.2%（P<0.05）、12.5%（P<0.05）、16.1%（P<0.05）,维吉尼亚霉素组较空白对照组提高了5.3%（P<0.05）;2.5、5、10 mg/kg那西肽组的日增重分别比维吉尼亚霉素组提高了3.1%（P＞0.05）、5.2%（P＞0.05）、8.6%（P<0.05）。日粮中添加了抗生素的组的料重比均显著高于空白对照组（P<0.05）,以10 mg/kg那西肽组最高。因此,在日粮中添加2.5～10 mg/kg那西肽对猪具有明显的促生长效果。 相似文献
8.
试验旨在研究加味理中汤方对脾虚泄泻模型小鼠血常规、血糖及脏器指数的影响。试验中复方由干姜、党参、白术、甘草、茯苓、山药、木香、桂枝、砂仁组成。选择无特定病原体级ICR小鼠72只,随机分为6组,每组12只小鼠,雌、雄各半。除空白对照组（C组）小鼠灌胃同体积的生理盐水外,其余各组小鼠灌胃番泻叶提取物9 d建立脾虚泄泻模型。试验第10 d停用番泻叶,恩诺沙星治疗组（A组）小鼠灌胃恩诺沙星水溶液,各中药组分别给予低剂量（6.5 g/kg）、中剂量（13.0 g/kg）、高剂量（26.0 g/kg）灌胃治疗,C组和泄泻模型组（M组）用同体积的生理盐水灌胃,连续治疗3 d。结果显示,与C组相比,M组小鼠空腹血糖含量、淋巴细胞百分比、肝脏指数均极显著降低（P<0.01）,血小板数目显著降低（P<0.05）,中性粒细胞百分比及白细胞数目显著增加（P<0.01）。与M组比,各治疗组小鼠中性粒细胞百分比极显著降低（P<0.01）,淋巴细胞百分及空腹血糖含量极显著升高（P<0.01）;中药高剂量组（ZH组）小鼠血小板数目显著提高（P<0.05）,肝脏指数极显著提高（P&l... 相似文献
9.
《黑龙江畜牧兽医》2020,(8)
为了研究黄芩苷对脂多糖(LPS)诱导后仔猪临床症状及血液学指标的影响,试验选取36头健康仔猪,分为空白对照组、LPS模型组、氟尼辛葡甲胺组、黄芩苷低剂量(25 mg/kg)组、黄芩苷中剂量(50 mg/kg)组和黄芩苷高剂量(100 mg/kg)组。各组猪在注射LPS前0.5 h给药,黄芩苷各剂量组及模型组猪按体重0.1 mg/kg腹腔注射LPS,空白对照组猪注射等量生理盐水,LPS诱导6 h后再给药1次。于注射LPS 3 h后测量各组猪的体温,并统计呕吐、颤抖、嗜睡猪的头数;于注射LPS后第3,24小时对各组猪采血,检测血常规指标和血液生化指标。结果表明:LPS诱导后第3小时,LPS模型组猪出现体温升高、颤抖、呕吐和嗜睡等临床症状,黄芪苷各剂量组猪的体温较LPS模型组均显著降低(P0.05),猪的呕吐和嗜睡症状减轻;LPS诱导后第3小时,与空白对照组比,LPS模型组白细胞数和血小板总数均极显著降低(P0.01);与LPS模型组比,黄芩苷中剂量组白细胞数极显著升高(P0.01)。LPS诱导后第24小时,与空白对照组比,LPS模型组白细胞数极显著升高(P0.01),血小板总数极显著降低(P0.01);与LPS模型组比,黄芩苷中剂量组血小板总数显著升高(P0.05)。LPS诱导后第3小时,与空白对照组比,LPS模型组总胆红素、肌酐、总蛋白、白蛋白和血糖含量出现显著或极显著变化(P0.05或P0.01);与LPS模型组比,黄芩苷中剂量组肌酐含量显著降低(P0.05);LPS诱导后第24小时,与空白对照组比,LPS模型组总胆红素含量、天门冬氨酸氨基转移酶活性、肌酐和血糖含量出现显著或极显著变化(P0.05或P0.01)。与LPS模型组比,黄芩苷低剂量组和中剂量组能极显著(P0.01)和显著(P0.05)降低总胆红素含量。说明50 mg/kg黄芩苷肌肉注射给药可以有效缓解LPS刺激导致的仔猪体温升高、白细胞数异常变化、血小板总数降低及肝脏和肾脏功能的损伤。 相似文献
10.
11.
Olsén L Ingvast-Larsson C Larsson P Broström H Bondesson U Sundqvist M Tjälve H 《Journal of veterinary pharmacology and therapeutics》2006,29(2):129-135
The pharmacokinetics and the effects on inhibition of histamine-induced cutaneous wheal formation of the histamine H1-antagonist fexofenadine were studied in horse. The effect of ivermectin pretreatment on the pharmacokinetics of fexofenadine was also examined. After intravenous infusion of fexofenadine at 0.7 mg/kg bw the mean terminal half-life was 2.4 h (range: 2.0-2.7 h), the apparent volume of distribution 0.8 L/kg (0.5-0.9 L/kg), and the total body clearance 0.8 L/h/kg (0.6-1.2 L/h/kg). After oral administration of fexofenadine at 10 mg/kg bw bioavailability was 2.6% (1.9-2.9%). Ivermectin pretreatment (0.2 mg/kg, p.o.) 12 h before oral fexofenadine decreased the bioavailability to 1.5% (1.4-2.1%). In addition, the area under the plasma concentration-time curve decreased 27%. Ivermectin did not affect the pharmacokinetics of i.v. administered fexofenadine. Ivermectin may influence fexofenadine absorption by interfering in intestinal efflux and influx pumps, such as P-glycoprotein and the organic anion transport polypeptide family. Oral and i.v. fexofenadine significantly decreased histamine-induced wheal formation, with a maximal duration of 6 h. A pharmacokinetic/pharmacodynamic link model indicated that fexofenadine in horse has antihistaminic effects at low plasma concentrations (EC50 = 16 ng/mL). However, oral treatments of horses with fexofenadine may not be suitable due to the low bioavailability. 相似文献
12.
13.
Xuting LI Sicong LI Bin WANG Min ZHANG Dingsheng YUAN Jinliang LI Ge LIANG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1338
Borneol is a traditional Chinese medicine. In Chinese veterinary clinics, borneol and its related compounds are often used in combination with florfenicol to treat respiratory infections. This study investigated whether the pharmacokinetics of florfenicol in rats was affected by its concomitant use with borneol. Sprague-Dawley rats were intragastrically administered borneol (50 mg/kg body weight (BW)) or 0.5% carboxymethyl-cellulose sodium for 7 consecutive days, and then intragastrically administered florfenicol (25 mg/kg BW) on the eighth day. Pharmacokinetic studies showed that borneol significantly decreased the area under the concentration-time curve from zero to infinity (AUC(0-t)), time to reach peak concentration (Tmax), and the peak concentration (Cmax) values of florfenicol, whereas the values of mean residence time from zero to infinity (MRT(0-t)), elimination half-life (t1/2z), apparent volume of distribution fraction of the dose absorbed (Vz), and plasma clearance fraction of the dose absorbed (CLz) were increased significantly. Furthermore, the mRNA expression levels of multidrug resistance 1 (MDR1) and cytochrome P450 3A1 (CYP3A1) in the jejunum and of CYP1A2 and CYP2C11 in the liver were significantly upregulated by borneol. In conclusion, borneol decreased absorption, increased clearance, improved distribution, and increased the mean residence time of florfenicol in rats, possibly through regulating the mRNA expression levels of drug-metabolizing enzymes and efflux transporters. 相似文献
14.
为了探讨脂多糖对肉鸡空肠中多药耐药相关蛋白2(MRP2)mRNA表达及转运功能的影响,采用荧光定量PCR方法检测脂多糖处理6 h和12 h后对健康肉鸡空肠组织中MRP2 mRNA表达的影响;采用小肠原位单向灌流试验测定了不同剂量脂多糖(5 mg/kg和15 mg/kg)对酮康唑空肠渗透性的影响。结果显示:经LPS处理6和12 h后,空肠中MRP2 mRNA的表达水平均极显著降低(P<0.01);鸡小肠原位单向灌流试验显示高浓度的脂多糖(15 mg/kg)能显著增加酮康唑在小肠的有效渗透率(Peff)(P<0.05)。试验表明,脂多糖能抑制肉鸡空肠组织中MRP2 mRNA的表达并对空肠的外排转运功能产生影响,从而促进其底物药物的小肠渗透性,进而可能会影响其体内生物利用度。该结果可为炎症状态下肉鸡的合理用药提供理论支持。 相似文献
15.
Kun Hu Gang Cheng Haixin Zhang Huicong Wang Jiming Ruan Li Chen 《Journal of aquatic animal health》2013,25(2):59-65
AbstractThe aim of this study was to analyze the influence of permeability glycoprotein (P-gp) gene expression on enrofloxacin (ENR) metabolism in aquatic animals. Nile Tilapia Oreochomis niloticus were fed different doses of ENR ranging from 0 to 80 mg/kg. The P-gp gene expression levels were determined by quantitative real-time PCR (qRT-PCR) at indicated time points after drug administration. Drug metabolism was determined by HPLC. The P-gp gene expression in liver and kidney was greatly enhanced 30 min after ENR administration at 40 mg/kg, peaked 3 h after drug administration, and then gradually decreased. Thirty minutes after a single oral administration of ENR (0, 20, 40, or 80 mg/kg), the P-gp gene expression increased in a dose-dependent manner. The P-gp gene expression levels in the kidney were significantly higher than those in the liver. Additionally, the metabolic rate of ENR in kidney was more rapid than that in liver. Furthermore, a close correlation was found between P-gp gene expression and ENR concentrations. These results suggest that P-gp may be involved in the ENR metabolism process in Nile Tilapia, providing a novel model for the potential utility of gene expression and drug metabolism studies in aquatic animals.Received July 9, 2013; accepted October 17, 2013 相似文献
16.
Kun Hu Xinyan Xie Yi-Ni Zhao Yi Li Jiming Ruan Hao-Ran Li 《Journal of aquatic animal health》2015,27(2):104-111
The aim of this study was to investigate the relationship between the administration of chitosan (CTS), expression of permeability glycoprotein (P-gp), and the metabolism of norfloxacin (NOR) in Grass Carp Ctenopharyngodon idella. Fish were administrated with a single dose of either NOR, CTS, 1:5 NOR–CTS or 1:10 NOR–CTS. The P-gp expression was analyzed by immunohistochemistry and real time-PCR. The concentration of NOR was determined using HPLC. The mRNA and protein expression of P-gp in the fish intestine was significantly enhanced following a single dosage of 40 mg/kg NOR, and peak expression occurred at 3 h after drug administration (P < 0.05). A single dosage of both 1:5 NOR–CTS and 1:10 NOR–CTS reduced the intestinal P-gp expression to levels significantly lower than that from NOR alone (P < 0.05), but significantly higher than that from the control (P < 0.05). Interestingly, CTS alone also led to a slight decrease in P-gp expression. In addition, pharmacokinetic assays revealed a marked increase in area under the curve (AUC) of NOR with 1:5 and 1:10 NOR–CTS, by approximately 1.5-fold and threefold, respectively. Finally, the relative bioavailability of NOR after a single oral dosage of 1:5 and 1:10 NOR–CTS was enhanced to 148.02% and 304.98%, respectively. In this study, we demonstrated that the transmembrane glycoprotein P-gp regulates NOR metabolism in the intestine of Grass Carp, suggesting that NOR may be a direct substrate of P-gp. More importantly, we showed that CTS can inhibit P-gp expression in a dose-dependent manner and improve the relative bioavailability of NOR in this species.
Received August 25, 2014; accepted November 12, 2014 相似文献
17.
18.
L.M. JOHNSON S.M. LANKFORD S.A. BAI 《Journal of veterinary pharmacology and therapeutics》1995,18(2):117-123
The disposition of intravenously (0.5 mg/kg) and orally (5 mg/kg) administered verapamil was studied in six dogs after 3 days' pre-treatment with verapamil alone (5 mg/kg, every 8 h) and during concomitant oral administration of cimetidine (16 mg/kg, every 8 h). Racemic verapamil and norverapamil, an active metabolite of verapamil, were measured by fluorescence high performance liquid chromatography using an achiral phenyl column. The isolated racemic verapamil was rechromatographed on an Ultron-OVM chiral column, which separated the two verapamil enantiomers. Cimetidine co-administration significantly reduced the systemic clearance of racemic verapamil as well as that of its enantiomers by 25–29%. The clearance of racemic verapamil administered orally as well as that of its enantiomers was also reduced by 28% during cimetidine coadministration. The decrease in verapamil metabolism by cimetidine appeared to be non-stereoselective. On the other hand, cimetidine co-administration had no significant effect on the apparent volume of distribution of racemic verapamil and its enantiomers or the plasma protein binding or the blood to plasma concentration ratio of racemic verapamil. In addition, the ratio of the area under the plasma concentration-time curve for norverapamil to that of verapamil was unaffected by cimetidine co-administration. These results suggest that cimetidine alters the disposition of verapamil by decreasing the hepatic blood flow rate and by inhibition of its first-pass metabolism. 相似文献
19.
将40只1日龄雏鸡随机分成低Se试验组（日粮Se含量0.032 mg/kg）和正常对照组（日粮Se含量0.229mg/kg）,分别在30、45、60和75 d断头处死取样,用ELISA法检测组胺浓度及半定量RT-PCR法测定空肠2型组胺受体（H2R）mRNA表达水平。结果表明,血清组胺水平在对照组与缺Se组及缺Se组组间比较差异均极显著（P〈0.01）;Se缺乏时鸡空肠H2R mRNA表达水平呈升高趋势,对照组与缺Se组及缺Se组组间比较差异极显著（P〈0.01）;在整个试验期间,缺Se组血清Se含量与组胺浓度及H2R mRNA表达水平呈时间-效应关系。缺Se刺激肥大细胞脱颗粒,使血清中组胺浓度和空肠H2R mRNA的表达水平升高,可能对硒缺乏引起的空肠组织损伤具有保护作用。 相似文献
20.
Sasaki N Yoshihara T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(2):211-213
Seven Thoroughbred horses were laparotomized and Force Transducers were fixed on the proximal jejunal and cecal serosa. After observation of the digestive tract motility in consciousness, cisapride (0, 0.5, 0.75 or 1 mg/kg) was orally administered. In horses treated with 0.75 mg/kg or 1.0 mg/kg cisapride, the migrating contraction (MC) of the jejunum was significantly increased in frequency. 相似文献