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1.
Four species of microalgae (Thalassiosira weissflogii, Thalassiosira pseudonana, Nannochloropsis sp. and an unidentified species) were isolated from natural seawater of East China Sea. By studying the effect on growth and survival rates of two sizes of postset juveniles of razor clam, Sinonovacula constricta (Lamarck, 1818) (shell length 0.37 ± 0.05 mm and 1.07 ± 0.08 mm, respectively), the food value of these microalgae and four other commonly used microalgal species in China (Chaetoceros calcitrans, Isochrysis galbana, Platymonas subcordiformis and Nannochloropsis oculata) in single and two algal species diets were evaluated and compared. For smaller juvenile, when used as a monospecies diet, C. calcitrans and I. galbana supported the greatest shell growth and the highest survival rate, and P. subcordiformis, N. oculata and T. weissflogii produced the smallest shell growth and the lowest survival rate. Meanwhile, for the larger juvenile, single‐species algal diet had the greatest and weakest food value on T. weissflogii and N. oculata, respectively. Full‐mixed algal diets exhibited the strongest food value and most of the two species diets supported more food value than corresponding individual single microalgae diet. Moreover, shell growths and the survival rates of larger juvenile fed most of the two species diets were lower than fed single species of T. weissflogii.  相似文献   

2.
Sufficient high‐quality microalgae are required for indoor nursery of juvenile Ruditapes philippinarum. However, culturing numerous microalgae to support clam feeding is a heavy burden on many hatcheries. The effects of detritus from the macroalgae Ulva pertusa, Chondrus ocellatus and Undaria pinnatifida on the growth, amino acid content and fatty acid profile of Rphilippinarum were assessed as potential substitute diets. The green microalga Tetraselmis cordiformis served as comparative diet. Results revealed that the clams ingesting distinct diets presented no significant differences in growth of soft tissues, but the nutritional component of these clams differed dramatically. The clams fed with Undaria + Tetraselmis had the highest content of essential amino acids and proteins. In addition, the clams fed with single macroalgal diets and mixed macroalgal detritus and Tetraselmis showed significantly higher or statistically equal levels in n‐3/n‐6 ratio and docosahexaenoic acid (DHA)/eicosapentaenoic acid (EPA) ratio with respect to Tetraselmis diets. The relative percentages of EPA and DHA in clams fed with Undaria were 28% and 63% higher than those fed with Tetraselmis, and the arachidonic acid abundances in clams ingesting Undaria + Tetraselmis and Tetraselmis were significantly higher than those in clams ingesting other diets. Together, the diets containing single Undaria or mixed Undaria + Tetraselmis produced Manila clams with nutritional advantages in terms of essential amino acids and polyunsaturated fatty acids. Thus, the detritus of macroalgae, especially Undaria, is an appropriate substitute diet, at least partially, for culture of nutrition‐improved R. philippinarum.  相似文献   

3.
The importance of dietary 20:5n‐3 (EPA), 22:6n‐3 (DHA) and 20:4n‐6 (ARA) for growth, survival and fatty acid composition of juvenile cockles (Cerastoderma edule) was investigated. Cockles of 6.24 ± 0.04 mm and 66.14 ± 0.34 mg (live weight) were distributed into three treatments where live microalgae diets were fed constantly below the pseudofaeces production threshold, for three weeks. Diets had distinct fatty acid profiles: high EPA (53% Chaetoceros muelleri + 47% Pyramimonas parkeae), no DHA (47% Brachiomonas submarina + 53% Tetraselmis suecica) and low ARA concentrations (73% P. parkeae + 27% Phaeodactylum tricornutum). Growth was positively affected by high EPA and low ARA diets, whereas no significant growth was observed for the no DHA diet. High mortality of cockles fed no DHA diet raises questions about its suitability for cockles. In balanced diets with EPA and DHA, lower concentrations of ARA do not limit growth. The impact of dietary fatty acids was evident in the fatty acids of neutral and polar lipids of cockles. In polar lipids of all cockles, there was a decrease in EPA, in contrast to an increase in DHA. The combination of EPA and DHA in a live microalgae diet was beneficial for the growth and survival of juvenile cockles.  相似文献   

4.
This study was conducted to investigate the effects of dietary docosahexaenoic to eicosapentaenoic acid (DHA/EPA) ratios on growth performance, survival and fatty acid composition of juvenile swimming crab (Portunus trituberculatus). Four isonitrogenous and isoenergetic experimental diets were formulated to contain different DHA/EPA ratios (0.70, 0.84, 1.06 and 1.25). There were three replicates (15 crabs per replicate) for each diet treatment. The crabs were fed (about 6–8% body weight) twice daily for 8 weeks. A good growth performance and feed utilization were observed in swimming crabs fed the diets with DHA/EPA ratios of 0.70 and 0.84. Crabs fed diet with 0.70 DHA/EPA ratio showed a significantly higher weight gain (WG) compared with the crabs fed the diet with DHA/EPA ratio of 1.25 (P < 0.05). The result of this study showed that the survival increased with decreasing the ratio of DHA/EPA. The DHA/EPA ratios in polar lipid from tissues were influenced by the dietary DHA/EPA ratios. Results of this study indicated that the growth performance and survival of juvenile swimming crab are correlated to the DHA/EPA ratio in the diets, and the best growth performance and survival were achieved with the ratios of 0.70–0.84.  相似文献   

5.
A 30‐day experiment was conducted to investigate the effect of phospholipids (PLs) on growth performance, survival, fatty acid profile and gene expression of the early juvenile (C1) swimming crab (Portunus trituberculatus). Five semi‐purified diets were formulated with graded PLs levels (0%, 1%, 2%, 4% and 8%). Each diet was fed to four replicates of crabs (30 crabs per replicate, initial weight: 8.4 ± 0.1 mg). In this study, crabs fed diets with 2%, 4% and 8% PLs had significantly higher survival rates than crabs fed with 0% and 1% PLs. Crabs fed diets with 0% and 1% PLs had significant lower weight gain (WG) than crabs fed with 8% PLs. But crabs fed with diets containing 2%, 4% and 8% PLs showed no significant difference in WG. Besides, the content of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in whole body was higher than that in diets, but the monounsaturated fatty acids (MUFA) content showed an opposite trend. The EPA and DHA content in whole body increased with the increase in dietary PLs. Moreover, the moulting number increased with the increasing dietary PLs levels, the lowest moulting number was observed in crabs fed diets without supplementation of PLs. The ecdysteroid receptor (EcR) was found to be significantly up‐regulated by PLs levels. This study demonstrated that 2% PLs could meet the requirement of early juvenile (C1) swimming crab based on the growth and survival. And 4%–8% PLs supplemented in diets could reduce the leaching of feed.  相似文献   

6.
The effect of microalga lipid composition on the reproduction of the mussel Mytilus galloprovincialis has been assessed to determine the best feeding strategy for producing large quantities of mussel seed. Three diets based on two microalgae Isochrysis galbana (clon T‐iso) and Chaetoceros gracilis were tested. Besides, hatchery groups were compared with mussels from natural populations. Lipid content and fatty acid profile of digestive gland and mantle of both sexes, eggs and microalgae were analysed and related to sexual maturation. Hatchery groups, specifically the one fed on T‐iso, showed better results in reproduction success, and these differences were reflected on tissue and egg lipid composition. Microalga fatty acid profile influenced tissues and sexes, and higher levels of 18:1n‐9, 22:6n‐3 (DHA) and 18:2n‐6 were detected in groups fed on T‐iso while higher level of 20:5n‐3 (EPA) and 16:1n‐7 in groups fed on C. gracilis. Evidence of synthesis capacity of EPA from 18:4n‐3 and DHA from EPA is detected comparing their levels and the mobilization between tissues. Egg fatty acid profile was influenced by the female diet, and differences among groups were detected and confirmed by PCAs.  相似文献   

7.
8.
Development and design of adequate feeding protocols on sea cucumber larvae are necessary for improvements on larvae growth, survival and production of competent juveniles. This work assessed the Holothuria arguinensis larvae growth and survival, fed with three different single microalgae diets of Chaetoceros calcitrans, Isochrysis galbana and Tetraselmis chui, and two microalgae diets based on a combination of C. calcitrans and T. chuii (C:T; 1:1) and C. calcitrans, T. chuii and I. galbana (C:T:I; 1:1:1) during 2 months. Holothuria arguinensis larvae fed with T:C:I, showed higher survival and larger larval and stomach sizes than the ones fed with the other diets. Comparing the results obtained from the single diets, H. arguinensis larvae fed with I. galbana grew and survived better during the first 5 days. However, higher growth was obtained on the larvae fed with C. calcitrans from day 5 to day 12; since this day, larvae fed with T. chuii showed the largest size. These results could be related to the different microalgae size and morphology, in addition to the nutritional value of the different microalgae used. However, no competent larvae that metamorphosed into doliolaria were obtained during this experiment. The tanks used were transparent, which could be causing light stress to the larvae, since competent larvae were obtained in opaque production tanks in our aquaculture installations during the same time period. The combined diet of C. calcitrans, T. chuii and I. galbana (C:T:I) could improve the growth and larvae competency of H. arguinensis.  相似文献   

9.
The main objective of this investigation was to study the lipid requirements of the early juvenile (C1) swimming crab (Portunus trituberculatus) based on growth performance, survival, moulting and fatty acid profile. Four test diets were formulated with graded lipid levels (3.63%, 6.70%, 10.72% and 13.91%). Each diet was fed to 4 replicates of crabs (30 crabs per replicate initial weight (8.4 ± 0.1 mg). In this study, crabs fed diets with 13.91% lipid had significantly (< 0.05) higher survival than crabs fed with 3.63% lipid, but no significant (> 0.05) improvement of survival was observed when dietary lipid increase from 6.70% to 13.91%. Crabs fed diets with 10.72% and 13.91% lipid had significant higher weight gain(WG) than crabs fed with 3.63% lipid. While crabs fed with diets containing 6.70%, 10.72% and 13.91% lipid showed no significant (> 0.05) difference in weight gain(WG). Moreover, the lowest moulting number was observed in crabs fed diets with 3.63% lipid, but there was no significant difference (> 0.05) among other groups. The content of LC‐PUFA and DHA in the crabs fed diets with 3.63% lipid was significant (< 0.05) lower compared to other groups. But there was no significant (> 0.05) difference in EPA and ARA content among all groups. The activity of lipase increased as dietary lipid level increased (from 6.70% to 10.72%). However, beyond 10.72%, a significant (< 0.05) decreased in lipase activity was observed. The regression analysis of weight gain data indicated that crab fed diet containing 10.47% lipid level is considered as optimum lipid level for its maximum growth and moulting process.  相似文献   

10.
Gonadic conditioning and maturation of queen clam Dosinia ponderosa fed three microalgae (Isochrysis galbana, Tetraselmis suecica and Chaetoceros calcitrans) at three temperatures (20, 25 and 30°C) were assessed. Histological analysis showed six gonadic stages, indicators of maturity. The evaluation included sex proportion, oocyte diameter and frequency of gonadic stages after 22 (middle of trial) and 44 days (end of trial). At day 22, around 60% of females conditioned at 20°C, reached stage III (advances grade of maturation) when fed T. suecica. At day 44, around 50% of females were on stage III, conditioned at 20 and 25°C and fed T. suecica and C. calcitrans. For males, the best combination was C. calcitrans at 20 and 25°C. The temperature of 30°C resulted inadequate for maturation, especially when fed the diatom Isochrysis galbana. High mortalities occurred at that temperature with the three microalgal diets, especially in females. The results permits conclude that T. suecica and C. calcitrans are good sources of feed for D. ponderosa and that the best range of temperature for gonadic development is 22–25°C. These results may be basic for a best management and culture of this clam.  相似文献   

11.
An 8‐week feeding trial was conducted to evaluate the effects of replacement of fish oil (FO) with blending vegetable oils (VOs) on growth performance, antioxidant enzyme activities and fatty acid composition in tissue of swimming crab Portunustrituberculatus. Five isonitrogenous and isolipidic diets were formulated to contain VOs (colza oil: palm oil: linseed oil = 4:2:1) to replace 0 (the control diet), 250, 500, 750 and 1000 g/kg of FO (defined D0, D25, D50, D75, D100). Three hundred juvenile swimming crabs (initial weight 2.34 ± 0.08 g) were randomly stocked and sorted into 300 individual rectangle plastic baskets in three cement pools. Each treatment has three replicates, one replicate has 20 swimming crabs, and each diet fed 60 crabs distributed in 60 baskets. The results indicated that crabs fed the control diet showed significantly higher survival, final body weight, per cent weight gain (PWG), specific growth rate and moulting frequency, crude protein and crude lipid contents in muscles than those fed the D75 and D100 VO diets (p < .05). Crabs fed the D25 VO diet showed significantly higher concentration of triglyceride, low‐density lipoprotein cholesterol and total protein, activities of superoxide dismutase, catalase and glutathione peroxidase (GSH‐Px) in haemolymph than those fed the control diet (p < .05). Fatty acid composition in hepatopancreas was positively correlated with dietary composition. In summary, based on the PWG, the optimal replacement of FO with VOs was estimated to be 250 g/kg. These findings demonstrated that swimming crabs make better use of FO than VOs.  相似文献   

12.
A 6‐week study was conducted to determine the effects of different lipid sources in pelleted diets on juvenile mud crab Scylla paramamosain. Five isonitrogenous and isolipidic diets containing 8% level of fish oil (FO), lard (LD), safflower oil (SO), perilla seed oil (PO) or mixture oil (MO; VFO:VSO:VPO = 1:1:1), and a live food of marine bivalve Potamocorbula rubromuscula as the control diet (CF), were fed to groups of 25 juvenile crabs (average initial weight 7.4 g, carapace width 3.5 cm) in triplicate. The results showed that crabs fed MO had the highest survival (< 0.05). The moisture content was significantly higher in crabs fed LD, SO and PO (< 0.05). Crabs fed SO exhibited the lowest crude protein and lipid (< 0.05). Ash contents were obviously lower in LD group (< 0.05). Highest total lipid in the hepatopancreas and muscle was in LD and FO group respectively. Glucose, total cholesterol and low‐density lipoprotein were higher while high‐density lipoprotein was lower (< 0.05) in LD group. Tissue fatty acid compositions were consistent with those in diets. FO and MO diets had the same depression effect like CF on fatty acid synthase activity and mRNA expression in the hepatopancreas. The results of this study indicated that FO and mixed oil are suitable for preparation of pelleted diets with better effects for juvenile S. paramamosain compared with live food, and the ratio of n‐6/n‐3 fatty acids in pelleted diets must be <1.  相似文献   

13.
Four microalgal species [Dunaliella tertiolecta, Phaeodactylum tricornutum, Tetraselmis suecica, Thalassiosira pseudonana], previously identified as poor single‐algal diets for juvenile geoduck clams, Panopea generosa, were selected to mix with two comparatively better food‐value microalgae [Isochrysis sp. (TISO), Chaetoceros muelleri (CM)] to estimate synergistic or non‐additive effects of mixed microalgae on the growth/survival of young juveniles of P. generosa. In addition, the effects of different TISO/CM proportions [75/25, 50/50, 25/75; ash‐free dry weight (AFDW) basis] on growth/survival were assessed. Shell length, wet weight, dry weight, AFDW and organic matter content were significantly increased when using mixed TISO diets compared with TISO alone. In contrast, there were no significant differences with these variables when comparing mixed CM diets with CM alone. Ultimately, the best diet in terms of length and weight enhancement was a previously identified optimal one of TISO/CM (50/50). Survival on day 23 (60.7–79.3%) was not significantly different among any of the 11 diets tested. There were no significant differences in shell length, wet weight, dry weight, AFDW and organic matter content among the three TISO/CM ratios tested, but these diets gave superior results to TISO and CM fed alone. Survival (60.7–74.7%) on day 23 in the three TISO/CM ratios tested did not vary significantly.  相似文献   

14.
Replacing dietary fish oil with DHA‐rich microalgae Schizochytrium sp. and EPA‐rich microalgae Nannochloropsis sp. for olive flounder (Paralichthys olivaceus) was examined. Three experimental isonitrogenous and isolipidic diets with lipid source provided by 50% fish oil (F50S50), 50% (M50F25S25) and 100% microalgae raw material (M100) respectively were compared with a soybean oil (S100) diet as control. Triplicate groups of olive flounder juveniles (16.5 ± 0.91 g) were fed the experimental diets, and a group was fed the control diets for 8 weeks in a recirculation system. Results showed feed efficiency and growth performance were not significantly changed when fish oil (FO) was totally substituted by soybean oil (SO) or microalgae raw material (MRM). The whole‐body composition, lipid content of liver and muscle, and lipid composition of plasma were not significantly influenced by the total substitution of FO by MRM. The polyunsaturated fatty acids (PUFA) content of muscle and liver declined in fish fed S100 diet, whereas it was not significantly reduced in fish fed M50F25S25 and M100 diets. The total substitution of FO by MRM not only maintained the levels of arachidonic acid, EPA or DHA but also increased n‐3/n‐6 ratio. In conclusion, MRM as the sole lipid source is sufficient to obtain good feed efficiency, growth performance and human health value in olive flounder juveniles.  相似文献   

15.
The brackish cyclopoid copepod Apocyclops royi is used in Taiwanese aquaculture industry as a prey for fish larvae. This study investigated the effects of seven microalgal diets, namely single‐species diets of Isochrysis galbana (ISO), Nannochloropsis oculata (NAN), and Tetraselmis chui (TET), two‐species diets (ISO+NAN, ISO+TET and TET+NAN), and a three‐species diet (ISO+NAN+TET), on the population growth, female fecundity and fatty acid composition of A. royi. For reproductive traits, the combination ISO+NAN was found to be the most supportive diet for both population growth and female fecundity. For nutritional value, copepods fed ISO and ISO+NAN were detected to have the highest content of docosahexaenoic acid (DHA) (18.99% and 10.73% total fatty acid, respectively) and, more importantly, a high DHA/EPA ratio (6.09 and 4.09, respectively). Additionally, a comparison of fatty acid composition between copepods and microalgae gives a tentative indication that A. royi may have the ability to synthesize long‐chain polyunsaturated fatty acids (PUFA) from short‐chain PUFA. Our findings illustrate that ISO+NAN is the most suitable microalgal diet for mass culturing A. royi because it increases productivity and enhances the nutritional value of the copepods for use as fish larvae prey.  相似文献   

16.
The microalgae Isochrysis galbana and Chaetoceros muelleri were cultured with seaweed extract (SWE) from six different seaweeds and each SWE was used either as a supplement or as an alternative media to conventional f/2 medium. Microalgae cultured with only the f/2 media acted as the control treatment. I. galbana and C. muelleri were then fed to Penaeus indicus larvae, either singly or in combination, for 8 days to evaluate its nutritional quality. Shrimp larvae fed on microalgae cultured in f/2 media supplemented with SWE from Ulva lactuca or Enteromorpha intestinalis moulted faster (P < 0.05) to the second mysis stage compared with the control. Survival, total length and growth rates were better in shrimp larvae fed on microalgae previously cultured in media supplemented with SWE. In general, shrimp larvae fed microalgae cultured entirely in SWE performed equally well compared with the control. Shrimp larvae fed on mixed microalgae (I. galbana + C. muelleri) showed significantly better growth, survival and development compared with larvae on either single microalgal diet. It was concluded that microalgae cultured with SWE could be used as a low‐cost alternative media in producing live food for the hatchery production of shrimp larvae.  相似文献   

17.
Six experimental diets were designed with two phospholipid (PL; 0% and 1.5%) and three fish oil levels (0%, 1% and 3%) to evaluate the effects of dietary fish oil and PL levels on growth, survival and fatty acid composition of juvenile swimming crab, Portunus trituberculatus. Diets were iso‐energetic and iso‐nitrogenous and each diet was fed to triplicate groups (initially weight, 24.88 ± 0.04 g per crab) for 59 days. Weight gain (WG) and specific growth rate (SGR) increased with dietary PL addition to 0% fish oil‐supplemented diets (P < 0.05). On the other hand, WG and SGR decreased with dietary PL addition to 3% fish oil diets (P < 0.05). Crabs fed PL supplemented diets had higher haemolymph low‐density lipoprotein cholesterol concentrations and muscle crude lipid levels (P < 0.05) than crabs fed a none PL supplemented diet. The percentage of highly unsaturated fatty acids (HUFA; % total FA) in both polar and neutral lipids fractions of muscle tissue only increased in case of PL addition to 0% and 1% fish oil‐supplemented diets (P < 0.05). HUFA levels in the neutral lipids fraction of the hepatopancreas increased by dietary PL addition at each dietary fish oil level (P < 0.05). In this study, both dietary fish oil and PL addition contributed to a high n‐3/n‐6 ratio in muscle and hepatopancreas of P. trituberculatus. In conclusion, PL addition is only meaningful with fish oil‐deficient diets, in which case it enhanced lipid transport and HUFA absorption efficiency, hence improving the nutritional value of the diet.  相似文献   

18.
  1. Identification, protection and enhancement of essential habitats are priority issues for management and restoration of exploited species. The shores utilized by Asian horseshoe crabs as nurseries were surveyed and the coastal habitat characteristics were described in the northern Beibu Gulf of China. Regression models were applied to explore species‐habitat relationships.
  2. Fourteen and ten nursery sites were identified for Tachypleus tridentatus and Carcinoscorpius rotundicauda populations, respectively. Xiacun and Jinhaiwan in the eastern region of the northern Beibu Gulf were the essential nurseries for T. tridentatus, whereas Shanxin and Jiaodong in the western part were the primary nursery shores for C. rotundicauda. These shores supported high densities (4–6 individuals/100 m2) of juvenile horseshoe crab populations.
  3. Mangrove and seagrass coverage area, coupled with sediment physico‐chemical parameters, particularly grain size, and the environmental heterogeneity of nursery habitats explained the distribution pattern of juvenile populations. Most juvenile populations were found along the outer fringe of mangroves in the small shallow estuary, particularly near outflows of tidal creeks with generally higher chlorophyll a and organic carbon contents. The distribution of high‐density juvenile populations of both species also overlapped with areas of seagrass patches.
  4. These findings highlight the importance of mangroves and seagrasses in the nursery habitat use of Asian horseshoe crabs. Preserving the estuarine habitats with these vegetation types and identifying the high‐use nursery sites should be prioritised in China and other Asian places to conserve the declining Asian horseshoe crab populations.
  相似文献   

19.
A feeding trial was conducted to evaluate the optimum dietary level of eicosapentaenoic acid (EPA, 20:5n‐3) based on growth and non‐specific immune responses in juvenile rock bream. A basal diet without EPA supplementation was used as a control, and six other diets were prepared by supplementing with 4, 8, 12, 16, 20 or 40 g of EPA per kg diet. The actual EPA concentrations of the diets were 0.5, 4.3, 8.5, 13.0, 16.8, 21.0 and 41.2 g of EPA per kg diet, and the diets were abbreviated as EPA0.5, EPA4.3, EPA8.5, EPA13.0, EPA16.8, EPA21.0 and EPA41.2, respectively. Triplicate groups of fish averaging 1.06 ± 0.01 g (mean ± SD) were fed one of the seven experimental diets at the apparent satiation for 8 weeks. At the end of the feeding trial, weight gain, specific growth rate and feed efficiency of fish fed EPA16.8, EPA21.0 and EPA41.2 diets were significantly higher than those of fish fed EPA0.5, EPA4.3, EPA8.5 and EPA13.0 diets (< .05). Superoxide dismutase activity of fish fed EPA16.8, EPA21.0 and EPA41.2 diets were significantly higher than those of fish fed EPA0.5, EPA4.3 and EPA8.5 diets. Fish fed EPA21.0 and EPA41.2 diets showed significantly higher lysozyme activity than did fish fed EPA0.5, EPA4.3, EPA8.5 and EPA13.0 diets. The broken‐line analysis of weight gain indicated that the optimum dietary EPA level was 16.7 g/kg diet. These results suggested that the optimum dietary EPA level in juvenile rock bream could be greater than 16.7 g/kg diet but less than or equal to 16.8 g/kg diet based on the broken‐line analysis and the ANOVA test of weight gain.  相似文献   

20.
Four iso‐nitrogenous and iso‐lipidic diets were designed to investigate the effects of dietary phospholipids (PL) levels (with 0%, 1%, 2%, and 4% PL supplementation) on growth performance, lipid metabolism, and antioxidant capacity in early juvenile green mud crab (Scylla paramamosain). There were three replicates of 28 crabs (initial body weight from 42.02 to 42.44 mg) for each diet treatment, and growth trial lasted for 8 weeks. At the end of the growth trial, there was no significant difference in survival among all treatments. Crabs fed diet with 2% PL obtained highest weight gain (WG) and specific growth rate than other crabs. The molting frequency was not affected by different dietary PL addition. Besides, the contents of whole body lipid and long chain highly unsaturated fatty acids significantly increased with elevating dietary PL levels. In the hepatopancreas, crabs fed diet with 0% PL had significantly higher malondialdehyde concentration than other crabs. And crabs fed diet with 2% PL obtained significantly higher superoxide dismutase activity than crabs fed diets with 0% and 4% PL. Moreover, the mRNA expression of Na+/K+‐ATPase was significantly down‐regulated with dietary PL supplementation over 2%. Based on the second order polynomial regression analysis of WG, 2.37% dietary PL level was the optimal demand for early juvenile S. paramamosain. Moreover, we found crabs fed diet with 2% PL obtained better antioxidant capacity than other crabs.  相似文献   

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