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Serological investigation of aborted sheep and pigs for infection by Neospora caninum 总被引：4，自引：0，他引：4
Serology for Neospora caninum was undertaken using direct ELISAs on sera from 660 aborted sheep and 454 breeding sows, which had aborted or were considered infertile. All ovine sera were further tested by indirect fluorescent antibody test (IFAT) for N. caninum, and a latex agglutination test (LAT) for Toxoplasma gondii was performed on 423 of the samples, including all those positive by ELISA. ELISA-positive porcine sera were tested by IFAT and an inhibition ELISA for antibodies to N. caninum and by LAT for T. gondii. Only 3 (0.45%) of the ovine sera were seropositive for N. caninum by both ELISA and IFAT whereas although 40 porcine sera were seropositive by ELISA all were negative by IFAT. The results suggest that environmental exposure to N. caninum occurs rarely in sheep and pigs. 相似文献
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In the present study the seroprevalence of the protozoan parasite Toxoplasma gondii infection in sheep was investigated in 6 regions of Lithuania. Blood samples were taken from 354 sheep and were tested using commercial ELISA method. The total seroprevalence of Toxoplasma gondii infection in sheep was 42.1%. Significant differences in seroprevalence were observed between age groups (P < or = 0.05). The results of this investigation suggest that the Toxoplasma gondii parasite is widely spread, and can be one of reasons of sheep abortion in Lithuania. 相似文献
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[目的]了解内蒙古部分地区放牧牛羊弓形虫病感染情况。[方法]采用间接血凝试验(IHA)对阿拉善盟及呼伦贝尔市随机采集的286份放牧牛羊血清样本进行弓形虫抗体检测。[结果]绵羊血清弓形虫抗体总阳性率为1.68%,其中,阿拉善盟阳性率为4.84%,呼伦贝尔市阳性率为0.56%;经统计学分析,两个地区的绵羊血清弓形虫抗体阳性率差异不显著(P>0.05);牛血清弓形虫抗体总阳性率为0。[结论]内蒙古主要牧区放牧绵羊存在弓形虫感染,应引起足够的重视,并制定相应的弓形虫病防治措施。 相似文献
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Serum samples from 704 animals from 54 Swedish sheep flocks were analysed by ELISA twice during 1 breeding season for antibodies to Toxoplasma gondii and border disease virus (BDV). An ELISA, originally developed for the detection of antibodies to bovine viral diarrhoea virus (BVDV) in cattle, was assessed on sheep sera and the results were compared with those obtained in a virus neutralization test. The correlation between the 2 assays proved good. Before breeding, 132 (19%) sheep in 42 flocks had antibodies to T. gondii and 7 (1%) sheep in 5 flocks were seropositive to BDV. During the observation period 4 sheep seroconverted to T. gondii and 13 to BDV, giving an incidence rate of 0.7% and 1.9% respectively. No clinical signs due to the infections were observed. In 5 flocks the frequency of barrenness, abortion or stillbirths exceeded 5%, 5% and 8%, respectively, but there was no evidence that this was attributable to the agents studied. The proportion of BDV-positive flocks was significantly higher among flocks that had been in contact with cattle than among those that had not. 相似文献
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Rossi GF Cabral DD Ribeiro DP Pajuaba AC Corrêa RR Moreira RQ Mineo TW Mineo JR Silva DA 《Veterinary parasitology》2011,175(3-4):252-259
Toxoplasmosis and neosporosis have been recognized as economically important diseases with considerable impact on the livestock industry. Considering the scarce information on the occurrence of Toxoplasma gondii and Neospora caninum infections in sheep from Uberlandia, Minas Gerais State, Brazil, this study aimed to investigate the frequency of antibodies against these parasites in sheep sera from this region by using different serological methods. A total of 155 sheep serum samples were analyzed by the indirect fluorescence antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) for the detection of IgG against T. gondii and N. caninum. Seroreactivity by IFAT showed 80% of samples with titers between 512 and 2048 for T. gondii (cutoff ≥ 64) and 78% presenting titers between 50 and 200 for N. caninum (cutoff ≥ 50). Seroreactivity by ELISA showed 75% of samples with ELISA index (EI) between 2.0 and 3.0 for T. gondii (cutoff ≥ 1.3) and 54% presenting EI between 1.3 and 2.0 for N. caninum (cut off ≥ 1.3). Discordant results by both tests were analyzed by immunoblot, resulting in a total seropositivity of 61% for T. gondii and 23% for N. caninum, with 41% to T. gondii only, 3% to N. caninum only, and 20% to both parasites. There was a significant positive association between seropositivity to T. gondii and age over one year (P<0.001), but such association was not found for N. caninum infection. In conclusion, as T. gondii and N. caninum infections are simultaneously present in sheep flocks of this region, it should be emphasized the importance to carry out a regular monitoring of Toxoplasma infection due to its high prevalence, its zoonotic potential and induction of reproductive disorders leading to economic losses. For neosporosis, sheep farmers should be instructed about the presence of the parasite in the flock, its risk factors and potential abortifacient role in sheep. Differential flock management could be valuable tool to establish the association of serological positivity and reproductive disease induced by N. caninum in sheep. 相似文献
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Katzer F Brülisauer F Collantes-Fernández E Bartley PM Burrells A Gunn G Maley SW Cousens C Innes EA 《Veterinary research》2011,42(1):121
Toxoplasma gondii seroprevalence was determined in 3333 sheep sera from 125 distinct sheep flocks in Scotland, with the majority of flocks being represented by 27 samples, which were collected between July 2006 and August 2008. The selected farms give a representative sample of 14 400 sheep holdings identified in the Scottish Government census data from 2004. Overall T. gondii seroprevalence, at individual sheep level, was determined to be 56.6%; each flock tested, had at least a single positive animal and in four flocks all ewes tested positive. The seroprevalence of sheep increased from 37.7% in one year old stock to 73.8% in ewes that were older than six years, showing that acquired infections during the life of the animals is frequent and that environmental contamination by T. gondii oocysts must be significant. The median within-flock seroprevalence varied significantly across Scotland, with the lowest seroprevalence of 42.3% in the South and the highest seroprevalence of 69.2% in the far North of Scotland and the Scottish Islands, while the central part of Scotland had a seroprevalence of 57.7%. This distribution disequilibrium may be due to the spread and survival of oocysts on pasture and lambing areas. A questionnaire accompanying sampling of flocks identified farms that used Toxovax®, a commercial vaccine that protects sheep from abortion due to T. gondii infection. Only 24.7% of farmers used the vaccine and the vaccine did not significantly affect the within flock seroprevalence for T. gondii. The implications for food safety and human infection are discussed. 相似文献
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弓形虫几乎可以感染所有的温血动物,是一种十分重要的人兽共患原虫病病原。牛羊弓形虫感染不仅影响其自身的发育和繁殖,对畜牧业发展造成一定的经济损失,更重要的是感染弓形虫的牛羊肉和奶可成为人类健康和生命安全的潜在威胁。牛羊弓形虫感染受多种因素(年龄、品种、养殖场管理及犬、猫和鼠的存在等)的影响,不同地区牛羊弓形虫感染的风险因素不尽相同。基于此,论文对弓形虫感染的检测方法、我国不同地区牛羊弓形虫的感染情况及影响弓形虫感染的因素进行综述,旨在为相应地区牛和羊的饲养管理及保障其奶和肉产品的安全供给提供参考。 相似文献
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Buxton D Maley SW Wright SE Rodger S Bartley P Innes EA 《Veterinary parasitology》2007,149(1-2):25-28
Ovine toxoplasmosis, caused by the intracellular protozoan parasite Toxoplasma gondii, was first described in 1954 and while the incidence of ovine infection is difficult to define, it has been reported that in the UK it is responsible for between 1 and 2% of neonatal losses per annum. Recent reports have suggested that sheep persistently infected with T. gondii may pass infection to the fetus in subsequent pregnancies more readily than previously thought. These data show a high proportion of both successful and failed pregnancies in sheep to be positive by PCR for T. gondii with a tendency for samples from certain genetic lines of Charollais sheep more likely to be positive than samples from other lines, suggesting that some sheep have a particular genetic susceptibility to T. gondii. 相似文献
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Shahiduzzaman M Islam R Khatun MM Batanova TA Kitoh K Takashima Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1375-1376
The seroprevalence of Toxoplasma gondii was examined in adult women and domestic animals used for meat products from the Mymensingh District, Bangladesh. Cattle, goats and sheep showed a high seroprevalence (12, 32 and 40%, respectively), while the sera from all fifteen women examined in the same area were seronegative. Considering that primary infection in women during pregnancy can cause abortion and congenital defects, accidental ingestion of T. gondii infected meat products from domestic animals represents a risk factor for adult women living in the same area. 相似文献
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During the period 1999-2002, we have analyzed 9639 serum samples and 815 aborted samples (670 fetuses and 145 placenta) from 964 ovine and caprine farms distributed over all Sardinia island. After abortion notification, sera collected at random from adult animals were examined to detect simultaneously IgG and IgM antibodies specific to Toxoplasma gondii by indirect immunofluorescence assay, whereas fetuses and placenta were analyzed by a single tube nested PCR assay. Specific IgG antibodies were detected in 2048 (28.4%) sheep and 302 (12.3%) goats, specific IgM antibodies were found in 652 (9%) sheep and 139 (5.6%) goats. From a total of 2471 ovine and 362 caprine fetal samples including muscle, liver, abomasum, spleen, brain and placenta, 271 (11.1%) ovine and 23 (6.4%) caprine samples were T. gondii PCR-positive. Although T. gondii DNA was amplified from different types of tissues, placenta was the tissue with the highest detection rate. On the one hand, these results indicate that the seroprevalence of T. gondii infection in sheep and goats is relatively high, on the other PCR results demonstrate that T. gondii has a significant role in ovine and caprine abortion. Adequate management might be useful and essential to control the toxoplasmosis in the sheep and goats herds of Sardinia. 相似文献
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Sera collected from 447 dairy cattle on 14 dairy farms were tested for Neospora caninum antibodies by use of an immunofluorescent antibody technique. Positive reactions with titres > or =1:200 were found in 63 (14.09%) of animals. Neospora positive sera were also tested for Toxoplasma gondii antibodies by using a commercial latex agglutination test. Antibodies to T. gondii were detected in 3 (4.76%) of 63 N. caninum positive sera. These results indicate that N. caninum infection is widespread among dairy cattle in Bahia state. 相似文献
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为了解我国牛羊弓形虫病流行情况,应用间接血凝试验（IHA）对河南、山东、山西、内蒙古、云南、贵州6省区151份牛血清、50份奶样、490份羊血清进行了弓形虫病血清流行病学调查。结果显示：151份被检牛血清和50份牛奶样品,弓形虫抗体均为阴性。490份羊血清弓形虫抗体总阳性率5.71％,其中母羊、公羊血清阳性率分别为4.03％和9.79％;山羊、绵羊、杂交羊血清阳性率分别为6.58％、4.81％和5.13％;阳性率最高的为公山羊（13.2％）,最低的为母绵羊（2.96％）。28份阳性羊血清中,75％的抗体滴度为1：64,25％的抗体滴度为1：256。1岁后的羊,随年龄增长,血清阳性率升高。 相似文献
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Sharif M Gholami Sh Ziaei H Daryani A Laktarashi B Ziapour SP Rafiei A Vahedi M 《Veterinary journal (London, England : 1997)》2007,174(2):422-424
Serum samples from 290 cattle, 400 goats and 588 sheep slaughtered for food in various areas of the Mazandaran province, Iran were tested for antibodies to Toxoplasma gondii by the indirect immunofluorescence antibody test (IFAT), from December 2004 to April 2005. Antibodies to T. gondii were found in 30% (120/400) goats and 35% (206/588) sheep and 0% (0/290) cattle, at a dilution of 1:16 or more for goats and sheep and 1:128 or more for cattle. The highest titres observed in cattle, goats and sheep were 1:64 (0.7%), 1:128 (1%), 1:64 (2%), respectively. These results indicate that T. gondii antibodies are widespread in the animal populations and suggest that toxoplasmosis is a widely spread zoonotic infection in northern Iran. 相似文献
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Sheep are important domestic animals in the Northeast region of Brazil due to their minimal rearing and maintenance costs, and to their production of both meat and milk. In animals, Toxoplasma gondii infection results in significant reproductive and economic losses. The epidemiology of toxoplasmosis in sheep in the Northeast of Brazil has been little studied; particularly in Rio Grande do Norte State. Sera from 102 sheep intended for consumption in Lajes were subjected to the Toxoplasma-ELISA test to detect anti-T. gondii specific IgG confirming a past infection. Of the total tested, 30 (29.41%) sera were positive for IgG with an increasing number of positive animals with advancing age. We used IgG avidity ELISA in 30 positive samples and observed that 6 (20%) had low avidity antibodies and 24 (80%) had high avidity antibodies. Epidemiological studies are required in order to identify sources of infection for these hosts as well as their impact on animal breeding in the region and risk of transmission to humans. 相似文献
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J P Dubey H P Hughes H S Lillehoj H R Gamble B L Munday 《American journal of veterinary research》1987,48(3):474-476
The passage of non-Toxoplasma antibodies from dam to fetus through damaged placenta was studied in sheep inoculated with Toxoplasma gondii. Six ewes were inoculated with chicken globulins and Leptospira bacterins 2 months before oral inoculation with Toxoplasma gondii oocysts. Ewes were euthanatized between 42 and 62 days after T gondii inoculation. Antibody titers against chicken globulins, Leptospira spp, Haemonchus contortus, Sarcocystis spp, and T gondii were measured in the maternal and fetal sera. All ewes became infected with T gondii and had grossly visible necrotic foci in the placentas, and T gondii antibodies were found in the fetuses and the ewes. Appreciable amounts of antibodies to Haemonchus contortus, Sarcocystis sp, Leptospira spp, and chicken globulins did not cross the placental barrier. Seemingly, serologic examination of the fetus was reliable for the diagnosis of ovine congential toxoplasmosis. 相似文献
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Twenty-nine of 200 (14.5%) ewes on a farm in Cobleskill, NY aborted or had dead lambs during the lambing seasons of 1985 and 1986. Thirteen of 15 ewes that aborted had high Toxoplasma gondii antibody titers (1,024), via the modified agglutination test, and T gondii was isolated from the tissues of 1 fetus. In the 1987 lambing season, 5 ewes aborted, but not because of T gondii infection. Toxoplasma gondii antibodies were detected in 73.8% of sera obtained from 592 ewes in January 1987, indicating enzootic toxoplasmosis on this farm. Seropositivity increased with age; 40.2% of 1-year-old ewes had detectable antibody vs 89.2% of 2-year-old ewes. 相似文献
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Sawadogo P Hafid J Bellete B Sung RT Chakdi M Flori P Raberin H Hamouni IB Chait A Dalal A 《Veterinary parasitology》2005,130(1-2):89-92
Toxoplasma gondii is a ubiquitous intracellular protozoan parasite transmitted by food. Concerning this parasite, there are few studies done in Morocco. In this study, 261 sera from sheep intended for consumption in Marrakech were subjected to the Toxoplasma ELISA based serology test for the detection of anti-T. gondii specific IgG confirming a past infection. Of the total tested 72 (27.6%) sera were positive for IgG. This result shows that the seroprevalence approaches the world average and is similar to what is found in other cities of Morocco. This has prompted us to investigate other animal species in the region in order to evaluate the degree of contamination by this parasite as well as the potential risk incurred on consumption of their meat. 相似文献