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红菜薹游离小孢子培养技术研究 总被引：1，自引：0，他引：1
对影响红菜薹游离小孢子培养的关键因素进行分析. 研究结果表明,不同红菜薹材料之间的小孢子胚诱导率差异很大;4℃低温预处理2 h时小孢子胚的形成具有促进作用;NLN培养基中添加生长素(NAA)和细胞分裂素(6-BA)对小孢子胚诱导率影响不大,添加浓度过大时诱导率反而降低;培养基中添加活性炭能提高小孢子胚诱导率;子叶型胚在15 g/L琼脂的培养基上直接成苗率最高,达56.7%;再生植株在MS和1/2MS+IBA(0.5 mg/L)中的生根能力最好,1/2MS生根效果其次. 相似文献
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对影响红菜薹游离小孢子培养的关键因素进行分析。研究结果表明,不同红菜薹材料之间的小孢子胚诱导率差异很大;4℃低温预处理2h对小孢子胚的形成具有促进作用;NLN培养基中添加生长素(NAA)和细胞分裂素(6-BA)对小孢子胚诱导率影响不大,添加浓度过大时诱导率反而降低;培养基中添加活性炭能提高小孢子胚诱导率;子叶型胚在15g/L琼脂的培养基上直接成苗率最高,达56.7%;再生植株在MS和1/2MS IBA(0.5mg/L)中的生根能力最好,1/2MS生根效果其次。 相似文献
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胡萝卜游离小孢子胚状体诱导技术优化 总被引：1，自引：0，他引：1
以10种基因型胡萝卜品种为试材,采用游离小孢子离体培养的方法,对影响胡萝卜小孢子诱导的因素进行了研究,以期为胡萝卜小孢子培养过程中的相关环节提供参考。结果表明:有3个基因型诱导出胚状体,不同基因型之间胚诱导率差异明显,表明基因型是影响小孢子胚诱导的重要因素;盛花期取材进行诱导的出胚率显著高于其它2个时期,表明盛花期为最佳取材时期;33℃处理2 d的出胚率最高,为胡萝卜小孢子胚诱导的最佳热激条件;激素最佳配比为0.5 mg·L^-1 2,4-D;培养基中添加一定量的活性炭有利于小孢子诱导产生胚状体,最佳浓度为0.2 g·L^-1。 相似文献
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菜心小孢子培养体系优化 总被引：1，自引：0，他引：1
《中国瓜菜》2020,(7)
为了优化菜心小孢子培养体系,以10个菜心品种为试材进行小孢子培养,研究基因型、不同培养基配方和植物生长调节剂对小孢子胚诱导率的影响。结果表明,基因型是决定菜心品种能否出胚的一个重要因素,且不同基因型试材间小孢子胚诱导率具有明显差异,其中'Cx1'Cx6'Cx8'经过小孢子培养后较易获得胚状体,而其他材料则没有获得胚状体,基因型的不同导致胚状体诱导率在0～2.10胚·蕾~(-1)之间;同一基因型,在不同培养基成分诱导条件下,添加活性炭对小孢子胚状体诱导率的影响较大,胚诱导率在0.03～2.10胚·蕾~(-1)之间;培养基中蔗糖质量浓度为130 g·L~(-1)时',Cx1'的胚诱导率最高;此外,在培养基中添加0.05 mg·L~(-1)6-BA和0.5 mg·L~(-1)NAA可以显著提高菜心'Cx1'胚状体诱导率。 相似文献
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以9个大白菜品种的花蕾为试材,研究了基因型、6-BA以及不同固体培养基对大白菜小孢子胚发生的影响,以期为其它十字花科蔬菜小孢子培养体系的建立提供技术参考。结果表明:不同基因型大白菜的小孢子胚诱导率具有明显差异,9个大白菜品种中有6个诱导出胚状体,‘北京特好吃火锅菜’出胚率最高,达到每蕾12.38胚;添加0.3mg/L的6-BA可以促进小孢子胚状体的形成,以‘北京特好吃火锅菜’提升效果最显著;添加0.01g/L活性炭的MS固体培养基比B5培养基更适于成熟胚状体发育成苗。 相似文献
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大白菜小孢子培养影响因素研究 总被引：9，自引：0，他引：9
以福田50、长快两个大白菜一代杂种为试材,对影响小孢子胚状体形成的几个因素进行了研究。结果表明,0.1~1.0mg·L-1的NAA对大白菜小孢子胚的发生有抑制作用,而且随生长素浓度增高抑制作用加大。2,4-D对小孢子胚的形成有强烈的抑制作用。较低浓度的细胞分裂素能促进胚状体的发生,而较高浓度则产生抑制作用,ZT的最适宜浓度为0.2mg·L-1,6-BA的最适浓度为0.4mg·L-1,且6-BA的作用大于ZT。生长素和细胞分裂素的综合影响是两者影响的叠加。NLN培养基是最适于大白菜游离小孢子培养的基本培养基。活性炭对大白菜小孢子胚的发生有抑制作用。 相似文献
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为克服耐抽薹大白菜游离小孢子培养中出胚难的问题，以23个耐抽薹大白菜品种为试材，探究不同基因型间小孢子胚胎发生的差异以及两性霉素-B、灰黄霉素对小孢子胚诱导的影响。结果表明：采用NLN-13培养基，23份耐抽薹大白菜材料中仅有3份诱导出胚，胚诱导成功率为13.0%，且不同基因型间小孢子出胚率存在显著差异，表明基因型是影响大白菜小孢子胚胎发生的重要因素。添加适宜浓度的两性霉素-B和灰黄霉素，23份耐抽薹大白菜材料的胚诱导成功率分别达到60.9%和82.6%，添加1.0～2.0 mg·L-1两性霉素-B和0.5 mg·L-1灰黄霉素时诱导出胚材料数量最多，胚诱导率最高可达0.467胚·蕾-1和0.733胚·蕾-1，表明培养基中加入抗生素能够在一定程度上促进耐抽薹大白菜小孢子胚胎形成。 相似文献
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活性炭和振荡培养对提高大白菜胚诱导率的影响 总被引：4，自引：0，他引：4
以11个大白菜品种(品系)为材料,采用NLN培养基进行游离小孢子培养,研究了活性炭和振荡培养对小孢子胚诱导和形成的影响。结果表明:11个大白菜品种有9个诱导出胚,每花蕾诱导胚数0.056～12.67个。在NLN-13培养基中添加活性炭(0.5g/L),有利于小孢子胚的诱导和形成,Y536和Y585胚诱导率分别提高了16.1和4.8倍,极难诱导出胚的材料Y516也诱导出胚;采用振荡培养方式(65r/min),Y531和Y445胚诱导率较静置培养分别提高了18.23%和30.91%,出胚时间提早2～4d。 相似文献
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Ill-When Sul Schuyler S. Korban 《The Journal of Horticultural Science and Biotechnology》2013,88(6):822-827
SummarySignificant effects of seven basal media and three carbon sources (sucrose, glucose and fructose) on the induction of adventitious buds from embryos of Pinus sylvestris L. were observed. Moreover, hyperhydricity of expiants and shoot regenerants was observed on basal media containing fructose, especially with half-strength Murashige and Skoog (MS), MS, and woody plant medium (WPM). Expiants grown on a Gresshoff and Doy (GD) medium with sucrose produced the highest frequency of regeneration (81%) and with no hyperhydricity observed of developing adventitious shoots. Among three cytokinins tested including BA, BPA, and TDZ (at four concentrations each), 5 μM BA resulted in the highest regeneration frequency and mean number of adventitious shoots per embryo. Shoot régénérants were elongated after transfer to a GD medium containing 2 g-l–1 activated charcoal and no growth regulators. After one month, rooting was induced on 10% of expiants. 相似文献
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菠萝叶基愈伤组织诱导体细胞胚 总被引：6，自引：1，他引：6
以菠萝[Ananascomosus(L.)Merr.]吸芽上的叶基部组织为材料,研究了2,4-D、NAA、BA、TDZ在菠萝愈伤组织诱导体细胞胚过程中的影响。结果表明,2,4-D在体细胞胚诱导过程中起着极为重要的作用,其适宜浓度为5mg/L,适当浓度(1mg/L)的BA在降低畸形胚比例和增加体细胞胚发生率方面有重要作用,但BA也会促进愈伤组织分化不定芽;低浓度的TDZ与BA效果相似,在不同生长调节物质处理组合中,MS+0.01mg/LTDZ+5mg/L2,4-D培养基的体细胞胚发生率最高(97%)。低浓度NAA(2￣5mg/L)利于非胚性愈伤组织的增殖,而高浓度的NAA虽能促进体细胞胚发生,但这种体细胞胚很难继续增殖,在萌发时只生根不发芽。成熟体细胞胚在MS+0.2%活性炭上30d时的转株率为22.7%。 相似文献
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WANG Rong QIN Hai-mei HUANG Hua-tuo LU Yu-lan LAN Yan WEI Gui-Jiang GUO Jin WEI Ye-sheng 《园艺学报》2018,34(1):136-140
AIM: To investigate the distribution characteristics of interleukin-22 (IL-22) gene rs2227485C/T and rs2227491A/G polymorphisms in Guangxi people and the distribution differences with other ethnic groups, and to explore the difference levels of common lipid indexes in different genotypes. METHODS: SNaPshot technique and DNA sequencing were used in 280 Guangxi persons to examine IL-22 genotypes and to analyzed the distribution frequencies of allele and genotype in these sites. The distribution frequencies in different sexes, and the differences between groups and diffe-rence levels of common lipid indexes in different genotypes were analyzed statistically. RESULTS: Three genotypes of CC, CT and TT were found in rs2227485C/T with the frequency distribution of 17.1%, 49.3% and 33.6%, respectively. No significant difference between different sexes of each genotype and allele frequency in the Guangxi population was observed (P>0.05). Compared with the distribution frequencies of genotype and allele in HapMap-TSI, HapMap-HCB, HapMap-JPT and HapMap-MEX, those in Guangxi population showed statistically significant differences (P<0.05). Three genotypes of AA, AG and GG were found in rs2227491A/G with the frequency distribution of 16.1%, 52.8% and 31.1%, respectively. There was no significant difference between different sexes of each genotype and allele frequency in the Guangxi population (P>0.05). The significant differences of genotype frequencies among Guangxi population, HapMap-TSI, HapMap-JPT and HapMap-MEX were detected (P<0.05). Compared with the other 4 populations, allele frequencies in Guangxi population had significant difference (P <0.05). There were significant differences in the levels of HDL-C and LDL-C among the 3 genotypes of rs2227491A/G. The level of HDL-C had difference between AG/AA genotype and GG genotype. In addition, the level of LDL-C had difference between AG/GG genotype and AA genotype (P<0.05). CONCLUSION: rs2227485C/T and rs2227491A/G polymorphisms of IL-22 gene have differences in different populations. The rs2227491A/G polymorphism may be associated with serum lipid levels. 相似文献
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Rapid in vitro multiplication and conservation of Garcinia indica: A tropical medicinal tree species
《Scientia Horticulturae》2005,106(4):539-553
A simple and efficient method has been developed for rapid regeneration of plantlets via adventitious bud differentiation on mature seeds of Garcinia indica (Thouars) Choisy, a medicinally important facultative apomictic tropical tree species. High frequency direct shoot proliferation was induced in seed segments cultured on Murashige and Skoog's medium supplemented with cytokinins (BAP, kinetin and TDZ) alone and in combination with auxin (NAA). Amongst the various combinations used, BAP proved to be the most effective. Multiple shoots formed within 4–5 weeks of culture. The shoot forming capacity of the seeds was influenced by the BAP concentration tested (5–50 μM) and optimal response was observed at different concentrations (12.5–50 μM) in different genotypes investigated. Significant differences were recorded in terms of percent response (27.78–100%) as well as average number of shoots per explant (3.49–57.67) among the four genotypes investigated. Elongation of the induced shoots was achieved on MS basal medium containing 0.2% activated charcoal. The induction medium had a profound effect on rate of bud elongation with shoots induced on lower concentrations of BAP showing as much as four-fold elongation within 4 weeks. Proliferating shoot cultures were established by repeatedly subculturing the shoot nodes on MS medium supplemented with 5 μM BAP. Maximum rooting (91.66%) occurred in shoots cultured on half-strength MS medium supplemented with 10 μM IBA. The plantlets were successfully transferred to soil after hardening; the time period from initiation of shoot buds to transplantation being 15–17 weeks. For in vitro conservation, the shoot cultures were maintained on medium supplemented with 0.5 μM BAP and the subculture duration could be enhanced up to maximum of 11 months. 相似文献
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The aim of this work was to study the effect of solid medium, developmental stage, embryonic age, cold treatment and additives to the medium on plant regeneration from microspore-derived embryos in four F1 hybrids of ornamental kale (Brassica oleracea L. var. acephala). The results showed that all of the cultivars responded best when the embryos were cultured in solidified B5 medium with 1% agar. Optimal regeneration was gained when cotyledonary embryos were cultured for 25 days. Cold treatment significantly improved plant regeneration with a frequency of up to 79.0% under 4 °C for 2 d or 5 d. The addition of 3.0 or 5.0 mg/L silver nitrate (AgNO3) increased the frequency of plant regeneration. In the Zhouyehongxin cultivar, the frequency of plantlet development reached 84.4%. The addition of activated charcoal reduced embryo hyperhydricity. 相似文献
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极早熟油桃幼胚离体培养成苗相关因素探讨 总被引：2，自引：0，他引：2
以极早熟油桃‘超红短枝’的幼胚为试材, 在离体培养条件下, 研究了不同胚龄、低温处理时间、蔗糖浓度、6-BA、GA3浓度配比及种皮等因素对幼胚发育和成苗的影响。结果表明: 果实发育56 d时, 平均PF值仅为0.086。在各处理中, 以低温处理80 d、糖浓度6%对幼胚的发育和成苗效果最佳。6-BA、GA3配比对经80 d低温处理的幼胚影响较小, 9 mg/L的6-BA对30 d低温处理的幼胚发育有一定的促进作用。在低温条件下, 带种皮培养对幼胚PF值增大和提高成苗率的效果明显好于不带种皮培养的。 相似文献