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1.
来自苹果的SSRs在蔷薇科植物资源上的通用性分析 总被引：9，自引：0，他引：9
用来自苹果基因组的6个SSR标记(CH02a10、CH02b07、CH02c11、MS01a03、CH01f12和CH03d11) 引物, 对蔷薇科植物的6属19个种的基因组DNA进行了PCR扩增, 以分析其在不同物种上的通用性。结果表明, 这些SSR引物几乎在所有的供试物种上都能扩增出与在苹果上大小相似的SSRs产物。可见, 这些从栽培苹果上开发的SSR引物可用于蔷薇科内更多跨种、跨属的种质资源的研究。但不同的SSR标记所表现出的多态性有较大差异。对本研究所用的几个SSR标记来说, CH01f12在供试物种间呈现的多态性最高。 相似文献
2.
《Scientia Horticulturae》2005,104(2):151-160
Random amplified polymorphic DNA (RAPD) markers were used to assess the genetic stability of 10 micropropagated plants regenerated through axillary buds of clonal apple (Malus pumila Mill.) rootstock MM106. Eleven random decamer primers were successfully used to analyse genomic DNA from mother plants and in vitro plant material. A total of 129 scorable fragments were amplified with an average of 11.73 bands per primer. Among them, 99 were monomorphic and 30 were polymorphic with 23.2% polymorphism. Among these 30, 12 were found monomorphic across seven plants and parent. Three plants could be regarded as off-types. Our results show that RAPD markers could be used to detect the genetic similarities and dissimilarities in micropropagated material. 相似文献
3.
苹果柱型基因的ISSR分子标记研究 总被引：15，自引：1，他引：15
以普通型苹果品种‘富士’和柱型苹果‘舞姿’以及其杂交后代的柱型与非柱型实生苗为试材, 建立了苹果的ISSR ( Inter-Simple Squence Repeat polymorphic DNA) 分子标记体系, 并将ISSR 标记用于苹果柱型基因Co 的遗传分析。结果表明, 在20μL 反应体系中各组分的用量为Taq DNA 聚合酶1U、Mg2+ 的浓度为2.5 mmol·L-1 、模板DNA 用量20 ng、引物浓度0.2μmol·L - 1及退火温度52 ℃, 80 %引物具有良好的扩增能力。调整模板DNA 的用量、引物浓度及退火温度能够优化苹果ISSR-PCR 扩增体系。从所筛选的65 个引物中获得了35 个ISSR 标记, 其中33 个标记呈现1∶1 分离, 可用于苹果柱型基因的遗传分析。 相似文献
4.
《Scientia Horticulturae》2004,102(4):375-386
Ipomoea trifida belongs to the family Convolvulaceae and is closely related to sweetpotato. In this study, we reported the microsatellites in I. trifida sequence database, and tested their transferability and polymorphisms for both sweetpotato and its related wild species.In the DNA database, 1425 sequences were registered for I. trifida. Sixty-one independent sequences were found to have microsatellite motifs and PCR primers were designed to amplify 15 microsatellites loci identified. Twelve primer pairs could amplify the expected product size and nine primer pairs showed polymorphisms among the three genotypes of I. trifida. These 12 functional primer pairs were used to assess the transferability and the level of polymorphism between sweetpotato cultivars and its related wild species. The transferability showed, 100% for I. batatas, 83.3% for I. tiliacea, 75% for I. triloba and 66.7% for I. Lacunosa, respectively. These markers also revealed high level of polymorphism between wild species and sweetpotato cultivars. 相似文献
5.
大白菜和紫菜薹自交系染色体组DNA的RAPD分析 总被引：17，自引：1，他引：16
用８个随机引物（１０ｂｐ）对２个紫菜薹自交系的３个单株和４个大白菜自交系的４个单株的染色体组ＤＮＡ进行ＰＣＲ扩增，共有４０条带分离清晰、明亮，其中引条带在７个单株中表现出差异，可作为遗传标记（ＲＡＰＤ标记）。４个大白菜自交系之间的平均差异条带数为１２．３，２个紫菜薹自交系之间为９．５，大白菜与紫菜薹之间平均有１５．５条带的差异。用７个引物在９３Ｗ０５－７（紫菜薹）和９３Ｗ５８－５（大白菜）之间检测出对个ＲＡＰＤ标记。从同一自交系的不同单株抽提的染色体组ＤＮＡ扩增出较一致的ＤＮＡ谱带，而不同自交系间扩增出的ＤＮＡ谱带差异很大，这表明此方法也可以象ＲＦＬＰ一样，用于大白菜和紫菜薹的分子标记。 相似文献
6.
The genetic relationships among 63 melon (Cucumis melo L.) genotypes collected from various regions of Turkey were determined by comparing their molecular ISSR, SRAP, and RAPD markers with those of 19 foreign melon genotypes to investigate the taxonomic relationships and genetic variation of Turkish melon germplasm. Total 162 polymorphic markers (69, 18, and 75 obtained from ISSR, SRAP, and RAPD primers, respectively) were used to define the genetic similarity among the melon genotypes by dendrogram or two and three dimensional scalings. The average similarity (SM coefficient) between any two pairs of accessions examined as estimated by molecular variation was 0.73 ± 0.48. Within-group genetic similarities ranged between 0.46 and 0.96. Related genotypes or genotypes collected from similar regions were partitioned to similar clusters. Southeastern Anatolian genotypes were distinctly apart from group inodorus and group cantalupensis (sweet) genotypes. This reinforced the position of Turkey in the secondary genetic diversity center of melon. The genetic diversity among Turkish genotypes (H = 0.28 and I = 0.42) was only a little less than that of the world accessions (H = 0.30 and I = 0.45). On the other hand, the percentage of polymorphic loci among Turkish melon genotypes (90.7%) was even higher than that of the world accessions (87.6%). 相似文献
7.
Sequence-related amplified polymorphism (SRAP) and morphological markers to estimate the genetic relations among forty pea varieties (Pisum sativum L.) were studied. Data on 15 morphological traits were collected and analyzed. A total of 162 polymorphic SRAP's bands were scored using seven combinations of primers. Cluster analysis and both principal component and principal coordinate analysis were carried out. The varieties were grouped in four clusters through procrustes generalized analysis. Relationships among varieties revealed by molecular markers were significantly correlated with those based on the agronomic traits, suggesting that the two systems give similar estimates of genetic relations among the varieties. Parents selection depend on the specific objectives in further breeding programs. 相似文献
8.
莲雾转录组SSR信息分析及其分子标记开发 总被引：3，自引：0，他引：3
对莲雾[Syzygium samarangense（Bl.）Merr. et Perry]开展转录组测序分析,共获得87 538条Unigene。利用MISA软件搜索1 kb以上的21 153条Unigene,共检测出11 641个SSR位点,分布于8 115条Unigene中,出现频率为55.03%,平均分布距离为4.08 kb。优势重复基序为单核苷酸、二核苷酸和三核苷酸,分别占总SSR的43.44%、30.98%和24.38%。二核苷酸重复基元中以AG/CT为优势重复基元,占总位点的26.99%,三核苷酸重复基元以CCG/CGG为主。利用Primer 3.0共设计出25 734对SSR引物。从94对有效扩增引物中随机选择35对引物,对30个莲雾种质进行多态性验证分析,其中25对（占71.43%）引物表现稳定可重复的多态性。利用UPGMA作图,将30份供试材料分为2类。利用莲雾转录组数据进行SSR标记开发能获得较高频率的SSR位点,且类型丰富,可为莲雾遗传多样性分析和遗传图谱构建提供更丰富可靠的标记选择。 相似文献
9.
《Scientia Horticulturae》2005,103(2):209-218
Peroxidases (EC 1.11.1.7) were extracted from fresh cladodes harvested from nine ecotypes of the cactus species Opuntia ficus indica Mill. growing as a collection, in Marrakech (South Morocco). Two enzyme fractions were obtained by a progressive solubility method leading to soluble peroxidases (S) and ionically wall-bound peroxidases (I). The preferred substrate for cladode peroxidases was determined to be o-dianisidine over 4-chloro-1-naphtol and guaiacol. Contrarily to roots, no guaiacol-based activity was found in cladodes. The late ecotypes 1‘Haddaouia’ and ‘Moussa’ showed a relatively high peroxidase ratio S/I (units g−1 fresh weight). When subjected to electrophoresis on polyacrylamide gels, S and I peroxidase fractions each exhibited two enzyme forms based on their electric charges in basic and acidic gel media. Acidic peroxidase forms, well separated on basic gels, showed two principal migration zones with great differences in their enzyme activities depending on the fraction types S and I. Acidic ionically wall-bound peroxidases exhibited fast, highly active isoforms with Rf values 0.42–0.58. Basic forms, represented essentially in fractions S and resolved on acidic gels, were typified by slow and fast isoforms with a double banded pattern in most ecotypes. Opuntia ecotypes collected from localities surrounding Marrakech exhibited fast basic soluble isoforms, while distant ecotypes from Marrakech, including late ones, were typified by a fast acidic ionically bound isoperoxidase of Rf 0.58. Possible roles of O. f. indica peroxidases in growth and their evaluation as markers in this cactus species are discussed in this study. 相似文献
10.
Rania Jbir Néjib Hasnaoui Messaoud Mars Mohamed Marrakchi Mokhtar Trifi 《Scientia Horticulturae》2008
The amplified fragment length polymorphism (AFLP) analysis of DNA was used to characterize 34 pomegranate cultivars. By using a combination of six primers, a total of 327 markers were scored with a mean of 57.5. The high percentage of polymorphic bands (ppb) of 94.7 and the resolving power (Rp) collective rate value of 129.14 were scored. Data proved that the tested primers were informative to discriminate among cultivars and to survey the genetic diversity in this fruit crop. It has been assumed that the local pomegranate germplasm is characterized by a typically continuous genetic diversity. The derived dendrogram proved that cultivars are clustered independently from their geographical origin and their denomination. In addition, AFLP permitted the generation of a nearly unlimited number of molecular markers that are reliable in differentiating the cultivars and/or the polyclonal varieties. 相似文献
11.
以“秦冠”ד富士”F1群体的158个株系为试材,得到最佳SSR- PCR反应体系:在20 μL总的反应体系中Taq DNA聚合酶0.5U、引物0.1μmol/L、Mg2+ 1.8 mmol/L、模板DNA浓度15 ng/μL、dNTPs 0.30 mmol/L.利用SSR分子标记,采用JoinMap 3.0软件构建“秦冠”ד富士”的遗传连锁图谱.结果表明:从340对SSR引物中筛选出49对具有多态性的引物,在群体中共获得75个SSR标记,其中17个不符合孟德尔遗传规律,其余27个位点可定位到10个连锁群上.对苹果杂交F1代早期落叶病进行抗性遗传分析,最后将抗早期落叶病基因定位到了遗传连锁图谱中的第10个连锁群上.与已经发表的苹果遗传连锁框架图对比结果表明,抗早期落叶病基因被定位在已发表图谱的第8连锁群上. 相似文献
12.
Weiguo Zhao Yuhuang Wang Tingting Chen Gaofeng Jia Xiaoming Wang Jinliang Qi Yanjun Pang Sisi Wang Zhonghai Li Yongping Huang Yile Pan Yong-Hua Yang 《Scientia Horticulturae》2007
Mulberry is a perennial and economically important plant that has traditionally been used for feeding the silkworm. Evaluating genetic relationship is important for long-term improvement in mulberry yield, quality and resistance, and for germplasm conservation and identification. Population structure and genetic diversity of 8 mulberry populations from different ecotypes in China were analyzed by ISSR markers. Twelve ISSR primers generated a total of 83 amplification products, of which 50 were polymorphic, revealing 60.24% polymorphism among 66 mulberry local varieties, the mean PIC value was 0.1469. The total heterozygosity (HT), heterozygosity within population (HS), diversity between populations (DST) were 0.1600, 0.0851 and 0.0749, respectively. The coefficient of population differentiation (GST) was 0.4683, indicating that the variations among populations and those within populations contributed 46.8% and 53.2% to the total heterozygosity, respectively. The gene flow (Nm) was 0.5678, suggesting that genetic drift between populations can caused local genetic differentiation and therefore, population divergence. The mean genetic similarity coefficient was 0.8456, genetic similarity coefficient among 8 mulberry populations ranged from 0.8441 to 0.9640, indicating that genetic diversity of different populations existed variation. A dendrogram of all 66 local varieties of mulberry based on the genetic similarity using ISSR markers was generated by UPGMA cluster method. In the dendrogram, most varieties from the same ecotype clustered together. 相似文献
13.
14.
苹果属种质资源亲缘关系的SSR分析 总被引：15，自引：1，他引：15
应用SSR技术对59份苹果属材料进行基因组的多态性分析,从20对引物中筛选出12对SSR引物扩增出176个等位基因,平均每个位点14.7个等位基因。位点杂合度为0.4039～0.7412,遗传多样性指数为0.6156。用3对引物(CH02a04、CH02g04和CH01f03b)即可区分全部供试材料。NTSYS软件进行相似系数计算,UPGMA法聚类分析将59份苹果属材料分为3大类群,即栽培品种、地方品种和新疆的野生苹果、野生种或其变种。栽培品种中具有相同亲本起源的品种被紧密地聚到了一起,相似系数较高;地方品种与新疆的野生苹果的聚类相互交错,呈现出新疆的野生苹果作为东亚基因中心的起源种与中国各地方品种在起源演化上的相关性;各野生种及其变种类型均聚到了一起,与传统的系谱基本一致。 相似文献
15.
辣椒抗根结线虫基因Me3的精细定位 总被引：1，自引：0，他引：1
以含Me3基因的抗根结线虫辣椒自交系‘HDA149’为父本,感根结线虫辣椒自交系‘8214’为母本,构建了一个2 133株的F2作图群体。采用群体分离分析法（Bulked Segregant Analysis,BSA）,构建抗、感病两个DNA池,对由两亲本筛选出来的285对引物进行扩增分析,发现引物SSCP_B322和EPMS658在抗、感池间具有稳定的多态性。继续用这两对引物对F2单株进行扩增,以JoinMap 3.0软件分析发现SSCP_B322和EPMS658标记位于Me3基因两侧,遗传图距分别为0.56 cM和1.33 cM。运用回交群体BC1和F3群体验证实了这两个标记,可有效用于辣椒抗线虫分子标记辅助育种,也为图位克隆Me3基因奠定了基础。 相似文献
16.
《Scientia Horticulturae》2005,105(2):283-290
Randomly amplified polymorphic DNA (RAPD) markers were used to identify cultivars of persimmon (Diospyros kaki Thunb.) and to estimate the phenetic relationships between Diospyros species. Long random primers (15 and 20 bases) were used to increase the effectiveness of recognizing polymorphism. Twenty-five of 34 (74%) long random primers amplified polymorphic bands of 25 persimmon cultivars, indicating that the effectiveness improved compared with RAPD analysis using 10-base primers. The 25 primers amplified a total of 444 polymorphic bands, which distinguished all 25 cultivars. RAPD markers (133) clarified the phenetic relationship between the six species and subspecies of Diospyros: D. lotus, D. lotus subsp. glabbra and D. taitoensis formed one group (lotus group), and persimmon was related in order of closeness to the lotus group, D. oleifera, and D. rhombifolia. 相似文献
17.
Veerendra K. Verma T.K. Behera A.D. Munshi Swarup K. Parida T. Mohapatra 《Scientia Horticulturae》2007
Ten inbred lines of ash gourd [Benincasa hispida (Thunb.) Cogn.] were crossed to produce 45 F1 hybrids (without reciprocal) which were evaluated along with the parents for 20 growth- and yield-related traits, in a replicated field trial. High level of heterosis was observed among the hybrids for most of the traits examined, including yield. These inbred lines were analysed by using 42 RAPD primers those produced 282 DNA marker bands. A total of 130 RAPD markers were obtained with a mean of 3.1 per primer, which in combination discriminated all the inbreds from each other. Pair-wise genetic distance measurements ranged from 0.07 to 0.31, suggesting a wide genetic diversity for these inbreds. These inberds were also analysed with five ISSR primers of which four were informative. Twenty-six ISSR marker bands were generated of which 11 were polymorphic with an average of 2.80 per primers. The percentage of polymorphic bands produced were higher in ISSR markers (>80%) than generated through RAPD markers (46%). Although the results indicated significant positive correlations of genetic distance with hybrid performance and heterosis, the RAPD based genetic distance measures and use of limited ISSR markers in this present study could not effectively predict hybrid performance in this crop. The genetic variation among ash gourd inbred lines examined, herein, defined a marker array (combined ISSR and RAPD) for the development of a standard reference for further genetic analyses, and the selection of potential parents for predicting hybrid performance and heterosis. 相似文献
18.
Soumaya Rhouma Sonia Dakhlaoui-Dkhil Ali Ould Mohamed Salem Salwa Zehdi-Azouzi Abdelmajid Rhouma Mohamed Marrakchi Mokhtar Trifi 《Scientia Horticulturae》2008
Random amplified microsatellite polymorphisms (RAMPOs) were used to assess genetic diversity among 30 date-palm cultivars and 10 male trees. Using 18 primers combinations, 197 bands were scored and 186 were polymorphic suggesting the high level of polymorphism among studied cultivars. Moreover, taking into account the high percentage of polymorphic bands (ppb), the resolving power (Rp) together with the polymorphism information content (PIC) scored values, all the tested primer sets contribute strongly in the discrimination of date-palm genotypes. In addition, the topology of the derived UPGMA dendrogram exhibited cultivars’ clustering made independently both from the geographical origin and/or from the sex of trees. The present data support the Mesopotamian origin of the date-palm domestication. Thus we assume that the used method is efficient to assess genetic diversity within date-palm cultivars. Data are discussed in relation with the opportunity of the RAMPO method to provide additional molecular markers suitable in the improvement of the date-palms germplasm characterisation. 相似文献
19.
萝卜过氧化物酶基因片段的cDNA克隆及花期表达谱分析 总被引：1，自引：0，他引：1
通过研究过氧化物酶(POD)活性在心里美萝卜花期的变化以及萝卜POD基因的结构,探讨POD的基因结构和功能之间的关系。测定了萝卜花期 不同时间各个部位中POD活性,用改进的半定量RT-PCR分析了过氧化物酶基因(Rsprx)的表达谱,并通过RT-PCR克隆过氧化物酶基因的cDNA片段。结 果表明,心里美萝卜花期不同器官中的POD活性具有明显的变化,地上部分和地下部分POD活性差异显著,在结荚期的木质部II中达到峰值（17.53± 0.35）×103 U•g-1(FW)。不同器官中Rsprx的表达差异明显,花蕾和荚中表达最强。从花蕾和荚中得到4个长度为400 bp左右的cDNA克隆片段,序列 比对结果显示,与棉花的花器官过氧化物酶基因Ghpod具有较高的同源性和结构相似性。因此认为POD与萝卜花期生长发育有一定的关系,且有多种 过氧化物酶基因在该过程中表达。 相似文献
20.
W. A. Roach W. O. Roberts 《The Journal of Horticultural Science and Biotechnology》2013,88(1):108-119
SummaryRandomly Amplified Polymorphic DNA (RAPD) markers were used to evaluate genetic similarity and inter-relationship among31 acid citrus species and cultivars, including sour oranges (six accessions); ‘Yuzu’ (four accessions) andits relatives (21 accessions). Out of the 60 decamer primers screened, 27 were selected which produced 108 markers; 76 of which were polymorphic. Species or cultivar-specific RAPD markers were also found. A dendrogram based on genetic distance implied that sour oranges were very distinct from ‘Yuzu’ and its relatives. ‘Yuzu’ accessions were very closely linked to each other, however; for the other specimens genetic polymorphism could easily be detected by RAPDs and the genetic variation between accessions was quite high and revealed their different origins. In this study some RAPDs allowed the distinction of very close cultivars, for instance ‘Kabosu’ from ‘Aka kabosu’. 相似文献