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1.
SRAP在葱栽培品种遗传多样性研究中的适用性分析 总被引：23，自引：2，他引：21
为评价SRAP技术对葱品种进行鉴定和遗传关系分析的适用性, 对20个葱栽培品种的表型特征进行了观察记载, 利用256个SRAP引物组合对其进行了遗传多样性研究。结果表明: (1) 256个SRAP引物组合中有161个引物组合产生多态性条带, 占所用引物组合数的62.9%。161个引物组合共产生336条多态性条带, 不同引物组合产生的多态性条带数为1～6个, 平均2.1个。20个葱栽培品种遗传相似系数变幅为0.464～0.938, 平均0.703。(2) 依据SRAP进行聚类分析的分类结果与依据表型特征分类的结果一致。上述结果说明SRAP标记可以在葱栽培品种的鉴定和遗传多样性研究中应用。 相似文献
2.
利用SRAP分子标记研究了6个切花菊品种及其2×4不完全双列杂交(NCⅡ)的38个F1代单株的遗传关系。结果表明,17对SRAP引物组合共获得229条带,其中多态性条带127条,平均每个引物获得7.5个多态性条带,多态性比率为56.0%,说明切花菊亲本品种及其杂交后代的分子多样性适中。6个亲本品种之间的Nei’s遗传距离介于0.11~0.25之间,平均为0.19,说明亲本品种之间的亲缘关系较近。亲本和杂交后代的遗传相似系数分别介于0.42~0.72和0.40~0.85之间,杂交后代遗传相似系数的中位数(0.61)高于亲本品种(0.55),说明杂交产生了一些变异株系,但是总的遗传基础有变窄或同质化趋势。基于遗传相似系数,UPGMA聚类将亲本和杂交后代划分为两大类,聚类结果与母本和杂交组合类型相符,说明SRAP分子标记可有效用于鉴定菊花不同杂交组合后代。 相似文献
3.
Li-Wang Liu Li-Ping Zhao Yi-Qin Gong Ming-Xia Wang Li-Ming Chen Jin-Lan Yang Yan Wang Fan-Min Yu Long-Zhi Wang 《Scientia Horticulturae》2008
Three molecular marker systems, RAPD (random amplified polymorphic DNA), ISSR (inter-simple sequence repeat) and SRAP (sequence-related amplified polymorphism), were employed for identification and genetic diversity analysis of 35 elite late-bolting radish cultivars. Detected by 35 RAPD primers, 22 ISSR primers and 17 SRAP primer combinations, the proportions of polymorphic bands were 85.44%, 85.2% and 85.41%, respectively, and the mean genetic similarity coefficients between pairs of genotypes were 0.781, 0.787 and 0.764, respectively. Each of the three molecular marker systems can identify all the cultivars. Five sets of three-RAPD primers, 3 sets of three-ISSR primers and 16 sets of three-SRAP primer combinations were able to distinguish all the cultivars. A linear relationship was observed between Resolving power (Rp) of a primer and its ability to distinguish genotypes. The 35 cultivars were clustered into three major groups based on the RAPD, ISSR and marker combination data with UPGMA, which are in high accordance with their own origins and main characteristics. The results demonstrated that these three marker systems could be useful for identification and genetic diversity analysis of radish cultivars. 相似文献
4.
Young Hoon Park Seong Gyu Ahn Young Mi Choi Hye Jeong Oh Dong Chun Ahn Jin Gi Kim Jum Soon Kang Young Whan Choi Byoung Ryong Jeong 《Scientia Horticulturae》2010
The ‘Genome database for Rosaceae (GDR)’ provides a large collection of expressed sequence tags (ESTs) harboring simple sequence repeats (SSRs) from several Rosaceae genera, including Rosa (rose). Primer pairs flanking SSR were designed for 312 unique Rosa ESTs based on GDR database. Eight rose (Rosa hybrida L.) genotypes were tested for PCR amplification, and 287 (92%) of the primer pairs generated allele-specific PCR bands that were readily scored. From 183 (63.7%) primer pairs that evidenced polymorphic alleles among the eight rose cultivars, 20 pairs evidencing EST sequence homology to known gene functions and high levels of polymorphism were selected and utilized for DNA fingerprinting and genetic diversity assessments of 47 rose hybrids. A total of 202 polymorphic bands were scored and generated unique fingerprints for each rose hybrid. The Nei–Li genetic similarity coefficients among 1081 pair-wise comparisons of 47 cultivars exhibited a broad range of genetic variations from 0.30 (‘Grand King’ and ‘Carnival’) to 0.99 (‘First Red’ and ‘Red Champ’). UPGMA cluster analysis divided 47 hybrids into five major groups and two sub-groups. The cross-species transferability of 273 EST-SSR primer pairs was evaluated using four genotypes of the strawberry, a genus member of the Rosaceae family. PCRs on the DNA samples of strawberry were successful for 165 primer pairs; among these, 123 pairs amplified 243 polymorphic bands. As surrogates of the marker transfer, the phenetic relationship among the four strawberry genotypes was evaluated. Genetic similarity coefficients varied from 0.78 (‘Maehyang’ and ‘Janghyee’) to 0.64 (‘Janghyee’ and ‘Pragana’). The results of cluster analysis showed that the three octaploid strawberry cultivars were quite similar, whereas the diploid ‘Pragana’ was related distantly at the genomic DNA level. The EST-SSR markers developed in the present study can be efficiently utilized for genetic diversity studies in Rosaceae. 相似文献
5.
Collection and characterization of all sorts of germplasm resources are required for the development of new cultivars. Molecular characterization is more reliable than morphological characterization. Here, we employed sequence-related amplified polymorphism (SRAP) markers to evaluate genetic variation in a diverse collection of 56 Solanum accessions. Fifty-five SRAP primer combinations were used and a total of 635 polymorphic bands were observed. Cluster analysis by the unweighted pair-group method with arithmetic averages based on similarity matrices indicated that there were three clusters: (i) S. melongena; (ii) S. aethiopicum; (iii) S. surattense. The coefficients of genetic similarity among all the accessions ranged from 0.04 to 0.96 with an average of 0.73, and averaged 0.78 among S. melongena accessions originated from China, indicating extensive genetic variation. These results demonstrated that SRAP can be efficiently used to estimate genetic diversity and analyze phylogenetic relationship. 相似文献
6.
The pedigrees of most rabbiteye blueberry (Vaccinium virgatum) cultivars can be traced back to four wild selections, ‘Ethel’, ‘Clara’, ‘Myers’, and ‘Black Giant’; thus, they result from a very narrow germplasm base and are highly related. Until now randomly amplified polymorphic DNA (RAPD) has been the only type of molecular marker used in rabbiteye blueberry. Here we have tested whether a type of sequence-tagged site (STS) marker which utilizes specific ∼20-mer primers from expressed sequence tags (ESTs) of highbush blueberry (V. corymbosum), called EST-PCR markers, are useful for genetic fingerprinting and relationship studies in rabbiteye blueberry. Of 44 EST-PCR primer pairs, from an assortment of genes expressed in flower buds of cold acclimated and non-acclimated plants, and shown to amplify polymorphic fragments among a collection of highbush genotypes, 40 (91%) resulted in successful amplification, and 33 of those (83%) amplified polymorphic fragments among the rabbiteye genotypes. The average number of scorable bands per primer pair was two. A dendrogram constructed from genetic similarity values, based on the EST-PCR marker data, tended to group siblings and parent/progeny together, generally agreeing with pedigree information. A group of 20 markers from five EST-PCR primer pairs distinguished all the genotypes in this study. These markers are as easy to generate and as affordable as RAPDs, but are based on actual gene sequences, and should have general utility for DNA fingerprinting, genetic diversity, and mapping studies. 相似文献
7.
Bermudagrass (Cynodon ssp.) germplasm is genetically diverse and widely distributed in the world. The study was conducted to identify and assess the molecular variation and relationship among 24 cultivars developed in China, Australia and the USA. Sequence-Related Amplified Polymorphism (SRAP) was applied to cultivars identification in this study for the first time. Thirty of the 90 SRAP primer combinations generated a total of 274 clearly bands encompassing 249 (91%) polymorphic. Each bermudagrass cultivar has its unique binary code and can be distinguished from the others. Three distinct clusters were obtained by unweighted pair-group method with arithmetic averages based on the polymorphic markers. Coefficients of genetic distance among the genotypes ranged from 0.57 to 0.97. The results demonstrated that SRAP marker is a stable molecular marker technique for the identification of bermudagrass cultivars and their genetic relationships. 相似文献
8.
基于SRAP分子标记的桂花品种亲缘关系研究 总被引：8，自引：0，他引：8
利用相关序列扩增多态性( SRAP) 分子标记, 以柊树[Osmanthus heterophyllus ( G. Don. )P. S. Green ] 和华东木犀(Osmanthus cooperi Hemsl. ) 为对照种, 研究了桂花(Osmanthus fragrans Lour. )88个品种、1个野生种的亲缘关系, 18对SRAP引物共获得296个位点, 其中248个为多态性位点, 多态性比率达83.78%。平均每对引物组合产生16.4个位点和13.8个多态性位点。其中, 银桂品种群的Shanon信息指数( 0.3412) 和遗传多样性指数( 0.2191) 最高。遗传变异估算表明: 桂花遗传分化系数为52.95% , 大部分变异存在于品种群之间, 说明品种群体间遗传分化高。聚类分析结果表明, 以遗传相似系数0.762为截值, 可将91份种质分成6类; 各品种群的品种往往聚在一起; 四季桂品种群与其他品种群遗传距离较远; 色质较深的金桂往往与丹桂品种群的多个品种聚在一起。基于SRAP分子标记的聚类结果与基于形态的传统分类学的结果基本相符。 相似文献
9.
Echinacea is an allogamous genus, thus its cultivars or populations are genetically heterogeneous. Using amplified fragment length polymorphism (AFLP) to estimate the genetic diversity of Echinacea is generally limited by the large number of individual plants and the higher cost that need to be processed. In the present study, effectiveness of several sizes of DNA bulking (10, 15, 20, 25 and 30 individuals) with 20, 36 and 55 primer pairs was compared using AFLP in determining the genetic diversity of Echinacea species. The results indicated that the use of bulked DNA-based AFLP analysis by using the selected eight primer pairs was capable of detecting genetic diversity between the tested Echinacea species, provided that the potential presence of low frequency variants was ignored and a possible bias in the estimates of genetic similarity was accepted. The assessments showed that a bulk of 15 individuals could detect sufficient AFLP variations at most genomic sites. Additionally, 20 primer pairs could generate sufficient polymorphic fragments to achieve high resolving power of AFLP for the tested Echinacea species. 相似文献
10.
Gladiolus is one of the important commercial flowers with a large number of cultivars. However, genetic relationships among its genotypes have not been reported. This study analyzed genetic relatedness of 54 gladiolus cultivars using amplified fragment length polymorphism (AFLP) markers. A total of 24 AFLP primer pairs with three samples were initially screened, from which 9 primer sets that showed clear scorable and highly polymorphic bands were selected for AFLP reactions. Fluorescence-labeled amplification products were subjected to electrophoresis and then analyzed using an automated sequencer. A dendrogram was constructed by the unweighted pair group method using the arithmetic average (UPGMA). The number of AFLP fragments generated per primer set ranged from 10 to 151 with fragment sizes varying from 50 to 450 bp. A total of 660 AFLP fragments were detected, of which 658 (99.70%) were polymorphic. All the primers except E-AGG/M-CTA displayed 100% polymorphism. All cultivars were clearly differentiated by their AFLP profiles. The AFLP data were compared with previously obtained RAPD data and combined to generate a common dendrogram. The first cluster was dominated with indigenously bred cultivars while the second was dominated with exotic cultivars. This shows that most of the exotic cultivars as well as indigenous cultivars are closely related with each other. However, two indigenous cultivars viz., Pusa Suhagin and Pusa Archana share genetic similarity with exotic cultivars. Among the genotypes selected for the investigation, Pusa Gunjan was identified as the most distinct genotype. The AFLP markers developed will help future Gladiolus cultivar identification, germplasm conservation and new cultivar development. The assessed genetic relationships among gladiolus cultivars may enhance the efficiency of breeding program by selecting desirable parents with reduced breeding cycle. 相似文献
11.
为了探明东北区甜菜单胚不育系的遗传基础和类群划分,使用SRAP分子标记技术对48份东北区甜菜单胚品系骨干材料的遗传多样性进行分析。筛选出21对多态性较高的引物组合对供试材料进行PCR扩增,共扩增出366条带,其中196条是多态性带,平均多态性条带比率是53.6%。平均遗传距离是0.3945,平均遗传相似系数是0.6740。利用MEGA3.1软件,在遗传距离0.20处,供试材料被分为5个类群。结果表明,东北区48份甜菜单胚品系骨干材料的遗传多样性较为丰富,利用各类不育系做亲本,将可获得杂交优势好的杂交组合。 相似文献
12.
Sequence related amplified polymorphism (SRAP) markers were used to detect molecular marker polmorphisms among 86 citrus and their relatives in Aurantioidea. Twenty-one SRAP primer combinations produced a total of 376 polymorphic fragments with an average of 17.9 per primer combination and an average polymorphism information content (PIC) of 0.86. The unweighted pair group method arithmetic average (UPGMA) analysis demonstrated that the accessions had a similarity range from 0.28 to 1.00 with a mean of 0.64. The subtribe Clauseninae (tribe Clauseneae) separated from the subtribes of the tribe Citreae. The subtribe Balsamocitrinae (tribe Citreae) was the most distant from the others. In the Citrinae, ‘primitive citrus fruit trees’ and ‘near citrus fruit trees’ groups did not clearly separate from each other but all genera in these groups were distinct. On the other hand, subgenus Papeda and subgenus Citrus were not separated clearly in the dendrogram. C. maxima, C. medica and C. reticulata separated into three distinct clusters in agreement with three ‘true basic species’ thesis. Similarity-based analyses supported the theory of few ancestral species in Aurantioidea. 相似文献
13.
西瓜杂交种遗传多态性的SRAP标记分析 总被引：48，自引：0，他引：48
利用新型分子标记SRAP ( Sequence2Related Amp lified Polymorphism) 进行西瓜杂交种遗传多态性的研究, 利用25个引物组合对当前生产上推广的20个西瓜杂交种进行扩增, 从中筛选得到20个多态性引物组合, 共产生135个多态性条带, 平均每个引物组合产生71.1个多态性条带, 显示了较高的多态性比率。聚类分析20个引物组合的扩增结果, 20份材料分为3大类, Jaccardps相似系数在0.29～0.86之间。 相似文献
14.
Fubing Lü Niu Yu Xiaolan Zhao Jianxia Zhang Wenfang Xiao Wenyan Li 《The Journal of Horticultural Science and Biotechnology》2013,88(5):486-492
SummaryA selfed progeny from Phalaenopsis ‘Frigdaas Oxford’, with yellow flowers and red-purple blotches, was established. Individual plants varied greatly in terms of the number, size, and distribution pattern of the red-purple blotches. Sequence-related amplified polymorphism (SRAP) markers were used to analyse 159 individual plants in the progeny, and 14 polymorphic primer combinations were selected from the 594 SRAP primer combinations tested. In total, 80 polymorphic bands were produced using these primer combinations. On average, each primer pair combination amplified 80.1% polymorphic bands. An UPGMA dendrogram and a principal coordinate analysis (PCA) showed that the 159 individual plants selected could be divided into 12 clusters, including a cluster that consisted of plants in which the petals and sepals were fully red-purple in colour. Plants in which the petals and sepals had large red-purple blotches were genetically closer to the latter cluster than were those plants in which the blotches were small, indicating that the SRAP markers could be used efficiently to identify genetic variation in a Phalaenopsis population with respect to flower colour. Furthermore, 45 unique genes identified by SRAP from the selfed progeny population were sequenced. These data suggest that SRAP markers for the pattern of pigmentation in the petals and sepals of Phalaenopsis may be used in breeding Phalaenopsis for specific patterns of flower pigmentation. 相似文献
15.
梅遗传多样性的SRAP分析 总被引：4，自引：0，他引：4
为探明梅种质资源间的亲缘关系,利用SRAP标记对梅135份样品进行了遗传多样性分析。17个引物组合共扩增出124个位点,其中多态性位点为106个,多态位点比率87.5%。每个引物组合扩增位点数在4 ~ 12个之间,平均每个引物组合扩增位点数为7.3;通过NTSYS软件计算得到的样品间SM相似系数介于0.556 ~ 0.958,平均值为0.733,显示梅资源间存在一定的遗传差异;根据SRAP扩增结果,利用UPGMA法构建树状聚类图,聚类分析将135份样品分为7组,聚类分析结果与梅种的形态分类系统基本相符。 相似文献
16.
Paolo Riccardi P. Emilio Casali Francesco Mercati Agostino Falavigna Francesco Sunseri 《Scientia Horticulturae》2011
The genus Asparagus is very large consisting of around 150 species found as herbaceous perennials, tender woody shrubs and vines. The cultivated species (Asparagus officinalis L., diploid) is a highly prized vegetable, grown in different environments ranging from cool temperate zones to deserts, Mediterranean climates and tropical areas. As a consequence, Asparagus breeders have developed different cultivars that differ for their morpho-agronomic traits, habit and ploidic status (few triploid and tetraploid cultivars are used). Several breeding methods are used for developing cultivars, among which a well developed in vitro anther culture technique produces homozygous clones useful for F1 hybrids constitution. A fluorescent based AFLP (amplified fragment length polymorphism) technique were applied with the aim to assess genetic diversity among a collection of 173 doubled haploid (DH) androgenetic clones, five Asparagus wild species and interspecific hybrids obtained among the cultivated species and two wild relatives. The average number of AFLP fragments generated per primer set was 105, varying in size from 50 to 550 bp. A total of 1054 AFLP fragments were detected, 20% of which were polymorphic. Genetic similarity based on DNA polymorphisms, showed that a few number of AFLP primer combinations are able to distinguish the cultivated DH clones from the wild species. Indeed, from one DH clone for each anther donors and the wild species were used to construct a dendrogram using Dice's coefficient and the unweighted pair group method with the arithmetic mean (UPGMA). Genetic distances among all DH clones were calculated using the C.S. Chord distance; and a neighbour-joining (NJ) consensus tree was constructed in order to support the breeder for parental genotype choice for asparagus hybrid constitution. 相似文献
17.
Genetic diversity is essential to crop improvement. However, lack of molecular markers prevents the understanding of genetic diversity in celery and related species. In this study, SRAP and SSR markers was firstly used to evaluate genetic variation in 68 accessions of Apium graveolens and related species. A total of 888 bands were generated by 40 primer combinations of SRAP and 32 bands were produced by eight SSR markers. Of the 920 bands, 95.1% were polymorphic between A. graveolens and related species, and 49.2% were polymorphic within A. graveolens. Cluster and structure analysis could distinguish local celery, celery, and related species. These results suggested that both SRAP and SSR technologies can be efficiently used to characterize genetic variation and analyze genetic relationship in celery and related species. 相似文献
18.
Dendrobium loddigesii Rolfe is an endangered perennial herb with ornamental and medicinal value. Due to habitat deterioration and human over-exploitation, it has suffered a significant decline in abundance. Determining the level of genetic diversity and pattern of population genetic structure of this species would be helpful for its conservation and management. In this paper, sequence-related amplified polymorphism (SRAP) markers were applied to assess the level and pattern of genetic diversity in seven populations of D. loddigesii. Seventeen SRAP primer combinations generated a total of 231 clear amplification bands encompassing 187 (80.95%) polymorphic bands. A high level of genetic diversity was detected (PPB = 80.52%, H = 0.2743, I = 0.4113) at the species level. There was a moderate genetic differentiation (Gst = 0.304) among populations. Two main clusters were detected by cluster analysis using the unweighted pair-group method with arithmetic average (UPGMA). Mantel test revealed that no significant positive correlation was found between genetic distances and geographic distances (r = 0.2302; P > 0.05). Recommendations for conservation of the endangered species resources are proposed. 相似文献
19.
海南椰子栽培品种的SSR标记分析 总被引：2，自引：0，他引：2
应用简单重复序列（SSR）标记方法,对海南的11个椰子栽培品种进行了遗传多样性分析。选取30对引物用于PCR扩增,有23对引物扩增出有效多态性片段136条,平均多态性百分率为95.77%,每对引物扩增出的带数2~12条不等,平均为6.17条,每个SSR位点的多态信息量（PIC）变化于0.173~0.896之间,平均为0.561。11份材料之间遗传相似系数变化范围0.061~0.861,说明海南椰子栽培品种之间存在丰富的遗传多样性。UPGMA聚类分析结果表明,11个椰子栽培品种分为四个类群和两个亚群。SSR标记反映出的品种间亲缘关系与形态学研究的分类结果并不完全吻合。 相似文献
20.
利用SRAP 标记对111 份芥菜种质的遗传多样性进行了分析,并记载了其形态特征。从300 对SRAP 引物中筛选出21
对多态性明显、条带清晰、反应稳定的引物组合,共扩增出150 条条带,其中106 条为多态性条带,多态性比率为70.67%。
SRAP 聚类分析结果表明,111 份芥菜材料的遗传相似系数在0.38～0.89 之间,分为5 大类和若干亚类。对芥菜的叶片性状及根、
茎、叶、薹的发育状况等农艺性状进行调查分析,结果表明111 份芥菜材料的遗传相似系数在0.39～1.00 之间,分为4 大类和
若干亚类。这两种方法的聚类结果基本一致,表明SRAP 标记可以应用于芥菜种质资源遗传多样性和亲缘关系研究。 相似文献