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WANG Xin-e ZHANG Cheng-he XUAN Shu-xin MAN Hong LIU Hai-he SHEN Shu-xing 《中国农业科学(英文版)》2008,7(6):656-663
Interspecific alien addition lines have played significant roles in gene mapping, intergenomic gene transfer and chromosomal homoeological identification between closely related species. Selection of alien addition lines was conducted by karyotype analysis and morphological observation with the reference of parents. Triploid interspecies hybrid （AAC, 2n = 3x = 29） was obtained from Brassica campestris ssp. chinensis var. parachinensis Qinglu 9601 （tetraploid, AAAA, 2n = 4x = 40） x B. oleracea var. alboglabra Baihua 9705 （diploid, CC, 2n = 2x = 18） by immature hybrid embryo culture in vitro. Five different alien monosomic addition lines （AA ＋ C2, AA ＋ C3, AA ＋ C4, AA ＋ C6, AA ＋ C7） were obtained from the backcross progenies of AAC x AA. Each alien monosomic addition line has some specific morphological characters. It is feasible to obtain alien addition lines from the progenies of AAC × AA by karyotype analysis and morphological observation based on the reference of parents 相似文献
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本试验通过对向日葵品种的染色体核型分析,得出2n=34=24m+6Sm(4SAT)+4St(4SAT)的结果。同已报道的某些向日葵品种染色体核型相比,最显著的差异表现在随体染色体数目上,本试验首次发现向日葵有4对染色体带有随体。 相似文献
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榛属（Corylus L.）植物抗寒性研究 总被引：3，自引：0，他引：3
应用电解质渗出率法、恢复生长法和组织褐变法对榛属４７份试材的抗寒性进行了分析研究。根据抗寒性和恢复生长后的萌芽百分率，筛选出１０个较抗寒的杂交品系，为杂交品系的区域试验和推广提供了依据。 相似文献
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在小麦及其亲缘属物种中进行赤霉病（Fusarium graminearum Schw.）抗性鉴定的结果表明，纤毛鹅观草[Roegneria ciliaris Trin （Nevski）（Agropyron ciliare Franchet，2n=28，SSYY]和鹅观草[R. Kamoji Ohwi （Agropyron tsukushiense（Honda）Ohwi var. Transiens （Hack.）Ohwi，2n=42，SSHHYY]是迄今为止筛选出的对赤霉病抗性最高的物种。为将这一抗性转移到普通小麦中去，进行了它们与普通小麦的属间杂交。运用杂种幼胚培养技术，成功地获得了纤毛鹅观草（♀）×普通小麦品种中国春（♂）、中国春（♀）×鹅观草（♂）以及鹅观草（♀）×中国春（♂）3个组合的属间杂种，其中后两个组合为迄今首次成功的报道。根尖细胞染色体计数表明前两个组合F#-1均具有预期的染色体数（分别为2n=35和2n=42），而在鹅观草（♀）×中国春（♂）F#-1中，除2n=42的预期类型外，还发现有2n=63的异常类型。预期类型F#-1形态均呈中间型，2n=63异常类型偏向于中国春。所有
F#-1均完全雄性不育。离体鉴定表明它们对赤霉病仍然表现高抗。花粉母细胞减数分裂中期Ⅰ平均染色体配对构型分别为：（纤毛鹅观草×中国春）F#-1，30.47Ⅰ+2.22Ⅱ+0.01Ⅲ；（中国春×鹅观草）F#-1，39.82Ⅰ+1.09Ⅱ；（鹅观草×中国春）F#-1预期类型（2n=42），39.76Ⅰ+1.09Ⅱ+0.10Ⅲ；异常类型（2n=63），27.11Ⅰ+17.63Ⅱ+0.09Ⅲ+0.08Ⅳ。用中国春回交，所有F#-1均获得了回交后代。 相似文献
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YU Xiao-xia ZHAO Ning LI Hui JIE Qin ZHAI Hong HE Shao-zhen LI Qiang LIU Qing-chang 《农业科学学报》2014,13(2):310-315
Sweetpotato （Ipomoea batatas （L.） Lam.） breeding is challenging due to its genetic complexity. In the present study, interval mapping （IM） and multiple quantitative trait locus （QTL） model （MQM） analysis were used to identify QTLs for starch content with a mapping population consisting of 202 F1 individuals of a cross between Xushu 18, a cultivar susceptible to stem nematodes, with high yield and moderate starch, and Xu 781, which is resistant to stem nematodes, has low yield and high starch content. Six QTLs for starch content were mapped on six linkage groups of the Xu 781 map, explaining 9.1-38.8% of the variation. Especially, one of them, DMFN 4, accounted for 38.8% of starch content variation, which is the QTL that explains the highest phenotypic variation detected to date in sweetpotato. All of the six QTLs had a positive effect on the variation of the starch content, which indicated the inheritance derived from the parent Xu 781. Two QTLs for starch content were detected on two linkage groups of the Xushu 18 map, explaining 14.3 and 16.1% of the variation, respectively. They had a negative effect on the variation, indicating the inheritance derived from Xu 781. Seven of eight QTLs were co-localized with a single marker. This is the first report on the development of QTLs co-localized with a single marker in sweetpotato. These QTLs and their co-localized markers may be used in marker-assisted breeding for the starch content of sweetpotato. 相似文献
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欧洲甜樱桃（Ceraus avium（L.）Moench）花芽分化的研究 总被引：1，自引：0，他引：1
欧洲甜樱桃 (Ｃｅｒａｕｓａｖｉｕｍ (Ｌ )Ｍｏｅｎｃｈ) ,俗称大樱桃 ,是北方成熟最早的落叶果树 ,素有“春果第一枝”的美誉〔1〕。它全身是宝 ,是栽培价值极高的果树。花芽是决定果树产量的主要因素 ,研究花芽分化规律是正确制定栽培技术的重要理论依据 ,有关大樱桃花芽分化规律 ,除姚宜轩〔2〕和Ｇｕｉｍｏｎｄ ,Ｃ Ｍ〔3〕报道过之外 ,其他未见报导。为此 ,我们对乌克兰大樱桃品种早红宝石在泰安地区的花芽形态分化进行了研究 ,以便进一步制定合理的增产措施、控制生长发育、达到稳产高产提供理论依据。1 材料与方法1 1 材料试验于… 相似文献
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本文报道了采用组织培养方法首次成功地获得了水飞蓟试管苗,试验结果表明,在选用的１／２ＭＳ、ＭＳ和改良ｗｈｉｔｅ３种愈伤组织诱导培养基中,筛选出１／２ＭＳ＋ＮＡＡ１．０＋ＢＡ０．５＋ＺＴ０．５为适宜的培养基。选用的芽诱导培养基为：１／２ＭＳ＋ＮＡＡ０．２＋ＢＡ２．０;不定根诱导培养基为：ＭＳ＋ＮＡＡ１．０。在根、节间、节部和叶等４种外植体中,筛选部为适宜外植体。 相似文献
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稻属（Oryza L.）的核质互作效应研究 总被引：8，自引：0，他引：8
用6个栽培稻品种为核供体亲本,以O. Barthii、O. Glaberrima野生稻种以及分别属于Indica、Javanica和Japonica亚种的6个品种为细胞质供体亲本,通过核置换回交8-12代,育成8×6个同核异质代换系。观察了全部代换系的11个农艺性状,研究其中7×6个代换系对20个稻瘟病菌株和15个白叶枯病菌株的感病性反应。结果表明,核质互作因素对所研究性状和两种病菌的反应都表现出显著的遗传效应;其中核质互作导致的雄性不育是一主导效应。研究的结果又一次为稻核质互作导致不育提供这样的证据：以进化上较低级的种质为胞质亲本更可能获得雄性不育。初步认为,胞质的多样性在杂交稻育种上较之在常规品种选育上更为重要和迫切。设想把细胞质导致核质互作雄性不育的这一胞质特性作为稻种分类的依据之一。 相似文献
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不同育苗基质对叶用莴苣（L.sativa var.crispa L.）苗生长的影响 总被引：7，自引：0，他引：7
系统地评价了蛭石，珍珠岩，砂，砻糠灰，煤渣，醋糠，木屑等基质在不同配合和配比条件下的有关基质处理在叶用莴苣育苗上的各自不同效果。研究结果表明，不同基质处理对出苗，成苗以及苗生长发育的影响具有显著的差异。 相似文献
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HUANG Jia-quan SUN Zhong-hai 《中国农业科学(英文版)》2005,4(9):714-720
The Arabidopsis ICEI （inducer of CBF expression 1） gene was cloned through RT-PCR of Arabidopsis cDNAs and introduced into the lemon （Citrus Limon （L.） Burm. F. cv. Eureka） genome using Agrobacterium-mediated transformation method. Epicotyl segments from in vitro grown lemon seedlings were co-cultivated with A. tumefaciens strain EHA 105 carrying the binary plasmid pMVICE1, whose T-DNA region contain ICEI gene driven by 35S CaMV promoter. Among 320 epicotyl segments inoculated, 71 explants responded and regenerated 51 elongated shoots. These shoots were subjected to an extra month of kanamycin exposure. In this way, the number of escapes reduced. Thirteen of 31 survived shoots formed roots and 7 were tested positive using PCR technique. Southern blot analyses confirmed PCR results and demonstrated that more than two copies of the ICE1 gene were integrated into the lemon genome. 相似文献
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以四川省的48个割手密为材料,采用垂直板状聚丙烯酰胺凝胶电泳进行3种同工酶分析。结果表明,在酯酶、过氧化物酶、谷氨酸草酰乙酸转氨酶中,以过氧化物酶分析效果最佳,可作为甘蔗野生资源种群划分的生化指标。根据过氧化物酶谱特征,供试样本出现19个类群,反映了四川割手密的多态性、遗传差异和地理分布的特点。 相似文献
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