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《山东畜牧兽医》2019,(12)
本研究旨在探索一种快速测定饲料中木质素(ADL)含量的方法,同时评价聚酯网袋法测定饲料中ADL含量的准确性。本试验采用聚酯网袋法、国标法(GB/T20805-2006)和ANKOM滤袋法三种方法,分别测定了米糠粕、豆粕、甜高粱、玉米秸、大豆秸、干豆渣、大麦麸、青贮玉米、稻壳粉、麦壳、国槐、苜蓿、玉米芯共16种饲料中ADL含量,比较不同方法的测定结果,对不同方法的准确度进行分析。结果表明:(1)聚酯网袋法与国标法的测定结果一致;两种方法测定结果呈强正相关,线性回归方程为:y=1.0029x-0.0779(r=0.9997)。(2)ANKOM滤袋法测定饲料中ADL含量的结果与国标法一致,二者呈强正相关,线性回归方程为:y=0.9701x+0.2972(r=0.9975)。聚酯网袋法测定饲料中ADL含量的方法具有测定时间短、效率高、成本低、准确度高等特点。 相似文献
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本文对饲料级磷酸氢钙标准(HG2636-94)检测方法中钙含量的测定、氟含量的测定存在的问题进行了探讨,并提出了解决方法.利用分散剂消除磷酸根对钙测定的干扰,EDTA直接络合滴定钙,方法快速准确;控制适当的试科量以保证氟检测结果的准确度. 相似文献
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依据NY/T 1461—2007《饲料微生物添加剂地衣芽孢杆菌》所提供方法,开展饲料中地衣芽孢杆菌检验的方法验证,确定该方法的可行性。经过菌落特性检验、革兰氏染色检验及生化鉴定,对方法的排他性进行确认;通过营养琼脂倾注法,对3个不同产品开展了方法的包容性进行了考察,对同一样品的重复测定6次,开展了检验方法的准确度考察方法的重复性相对偏差在0～21%,中间精密度相对偏差为13.5%。通过确认表明该检测方法结果准确可靠,重复性好,适用于本实验室开展饲料中地衣芽孢杆菌的质量检验要求。 相似文献
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我们评价饲料蛋白质的营养价值通常依据蛋白质和氨基酸含量的高低,事实上这是不准确的。因为饲料蛋白质的营养价值不仅取决于氨基酸的含量,更取决于氨基酸的利用程度,即饲料氨基酸的生物学价值。通常用氨基酸利用率、氨基酸代谢率、氨基酸消化率来表示。但对鸡来说,由于粪尿很难分开,且尿中排出氨基酸量少,所以我们测定鸡对饲料的氨基酸消化率实际上是利用率,在实际生产中往往把这两者等同起来,尽管这不很准确。下面就饲料氨基酸利用率的若干问题作一综述。一、氨基酸利用率的测定方法测定氨基酸利用率的方法很多,可分为体内法和体… 相似文献
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饲料中种钙含量测定方法的比较研究 《畜牧与饲料科学》2016,37(10):38-38
试验采用高锰酸钾法（GB/T 6436-2002）、乙二胺四乙酸二钠络合滴定法（EDTA法,GB/T 6436-2002）和均相沉淀法对11种饲料原料进行钙含量测定的比较研究。结果表明,对饲料中的钙含量进行测定,3种方法所获得的结果差异均不显著（P〉0.05）。其中,高锰酸钾法准确度高于EDTA法和均相沉淀法,但过程复杂、时间长;EDTA法简单、快速,但测定结果不好判断;均相沉淀法比较生僻,很少在实际生产中应用。 相似文献
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应用Sibbald的TME(真代谢能)生物学评定法,测定了胶原蛋白饲料的真氨基酸利用率(TAAA),现将测试结果报告如下。 1 材料与方法试验动物:同品种43周龄成年公鸡6只。真氨基酸利用率的测定方法:用TME方法测定供试饲料和排泄物的氨基酸含量后,按下 相似文献
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牛结核ELISA方法的改进和优化研究 总被引：1，自引：0，他引：1
在传统ELISA基础上，对ELISA板的稳定系统、血清稀释液的显色系统、ELISA底物等流程进行了改进，建立了牛结核ELISA方法。阻断试验和交叉反应结果表明该ELISA方法具有较好的特异性；不同人多次重复试验，证明本方法的重复性好。 相似文献
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旨在建立蓝舌病病毒（BTV）血清学ELISA抗体检测方法,本研究以原核表达并纯化的BTV NS4重组蛋白为包被抗原,通过反应条件优化,建立了一种BTV重组NS4蛋白的间接ELISA抗体检测方法。SDS-PAGE结果显示,获得大小约52 ku的NS4重组融合蛋白,主要在上清中存在,Western blot显示,纯化后的重组蛋白具有良好的抗原性。通过方阵试验进行了ELISA反应条件优化,确定了重组蛋白抗原最佳包被量为3.0 μg·孔-1;血清最佳稀释倍数为1：200,酶标二抗最佳工作浓度为1：4 000,临界值分别为0.29和0.35。上述以NS4蛋白作为包被抗原建立的BTV抗体间接ELISA方法检测敏感性可达1：1 600;批内和批间重复性变异系数均小于10%;检测76份重庆地区牛群血清样品,阳性符合率为98%,阴性符合率为100%。本研究建立的间接ELISA方法为临床BTV血清抗体检测及BTV血清流行病学调查奠定了基础。 相似文献
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YI Huashan ZHAO Yao MA Xianping LI Huan XIA Yu ZHU Wenqing LIU Qianru CHEN Siqian CAO Huizhen YAO Ting GAO Lixu ZHANG Jinyang YANG Fahui WEI Xiaorong LI Qianyong XIE Yuanbing 《畜牧兽医学报》1956,51(12):3133-3140
This study was conducted to establish an indirect ELISA method for the detection serological antibody of bluetongue virus (BTV). The purified BTV recombinant NS4 protein obtained from the prokaryotic express system was used as the coated antigen, and then an indirect ELISA antibody detection method of BTV was developed by optimizing the reaction conditions. SDS-PAGE results showed that the recombinant NS4 protein with a size of about 52 kDa was obtained, which mainly existed in the supernatant. Western blot results showed that the purified recombinant NS4 protein had good antigenicity. The ELISA reaction conditions were optimized by the square matrix test. The optimal coating amount of recombinant NS4 protein antigen was determined to be 3.0 μg per well, and the optimal dilution ratio of serum to be tested was 1:200, and the optimal dilution concentration of HRP-labeled rabbit anti-cow IgG secondary antibody was 1:4 000, and the critical values were 0.29 and 0.35, respectively. The detection sensitivity of the BTV antibody was up to 1:1 600. The intra-assay repeatability and the inter-assay repeatability coefficient of variation were less than 10%. The positive coincidence ratio and negative coincidence ratio were 98% and 100% respectively. The indirect ELISA method established in this study laid a foundation for clinical serum antibody detection and serum epidemiological investigation of BTV. 相似文献
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Glaeser H 《DTW. Deutsche tier?rztliche Wochenschrift》2004,111(5):193-195
When applying methods for the detection of pathogenic micro-organisms in foodstuffs, information on the distribution of the target organism in the foodstuff submitted to a test should be available. The sampling plan used should allow to detect the presence of low levels of the target organism with a high probability. The individual steps of a detection procedure (pre-enrichment, selective enrichment, isolation and confirmation) need careful examination. It is important that inoculation of low levels of the target organism leads to successful enrichment even in the presence of relatively large numbers of competing organisms. In cases where competing micro-organisms form suspect colonies, there is a risk that false-negative results are obtained, because colonies of the target organism may not be isolated. Collaborative trials have to be carried out to assess the performance of presence/absence tests. Meaningful results are obtained only, if the test samples contain low levels of the target organism and if the effect of competing micro-organisms is checked. While it can hardly be disputed that the determination of the sensitivity and specificity of the test method provides valuable information, this cannot be said for "accordance" and "concordance", two recently proposed parameters which correspond to repeatability and reproducibility in quantitative tests. A better alternative may be to specify the probability to obtain two positive results, when analysing samples containing the target organism under repeatability or reproducibility conditions. In an analogous way, the probability to obtain two negative results with samples containing competing micro-organisms, but not the target organism, could be specified. 相似文献
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为了建立一种能快速检羊梅迪-维斯那病毒(MVV)的实时荧光定量PCR,根据GenBank中MVV gag基因的序列,设计了1对特异性引物及TaqMan探针,经过反应体系及条件优化,以定量的10倍系列稀释的阳性质粒为标准品进行实时荧光定量PCR扩增,建立了MVV实时荧光定量PCR。结果显示,该方法对MVV DNA最小检出量为10copies/μL,比普通PCR具有较高的检出率;组内及组间变异系数均低于2%,具有较好的重复性;该方法可以特异地检测到MVV,与其他病毒性样品无交叉反应。被检的92份临床样品中,实时荧光定量PCR和常规PCR的阳性检出率分别为4.3%和3.3%。为羊MVV快速检测和分子流行病学调查提供了一种有效的方法。 相似文献
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蚕用消毒剂亚迪欣的产品质量检测分析 总被引：2，自引：0，他引：2
亚迪欣是一种新型蚕用消毒药剂,主要有效成分为三氯异氰尿酸、磷酸三钠。为加强该消毒药剂的生产质量管理,进行了产品的质量检测分析试验。药剂中三氯异氰尿酸的有效氯含量检测方法经对照试验、精密度及重复性试验,其相对标准差(RSD)分别为0.02%、0.10%及0.12%,且在0.418~2.338 mg/mL范围内线性关系良好,R2=1;加入磷酸三钠的平均回收率为100.1%、RSD=0.16%,说明加入磷酸三钠对三氯异氰尿酸的有效氯含量检测没有影响。磷酸三钠在密塞条件下放置24 h检测其含量基本不变,在干燥条件下的质量分数增加1.08%,在潮湿条件下的质量分数减少1.41%。试验结果显示对亚迪欣产品有效成分含量的检测方法及标准制订合理可靠,产品质量可控性强。 相似文献
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为建立骆驼斯氏副柔线虫病间接ELISA （iELISA）诊断方法,本试验对骆驼斯氏副柔线虫半胱氨酸蛋白酶CPR基因进行重组表达,将获取的重组蛋白（rCPR）进行纯化和Western blotting检测,然后以纯化好的重组蛋白为抗原,通过棋盘滴定试验优化了抗原包被浓度、包被条件、抗体稀释度、酶标二抗稀释度、封闭液和封闭时间等,建立了骆驼斯氏副柔线虫病iELISA诊断方法,并对建立的iELISA检测方法进行了重复性、敏感性、特异性试验和临床检测。结果显示,抗原最佳包被浓度为8 μg/孔,血清最佳稀释度为1：50,酶标二抗最佳稀释度为1：5 000,最佳包被条件为4℃包被过夜,最佳封闭条件为3% BSA封闭2 h。临界值为0.235,待检血清D450 nm值>0.235则确定为阳性。重复性试验中变异系数均<10%,重复性较好;用该方法检测阳性血清的敏感性为96.3%;此方法仅与骆驼斯氏副柔线虫病阳性血清发生特异性反应,与感染了其他寄生虫的阳性血清无交叉反应,特异性为100%;对140份临床血清进行检测,阳性率为86.4%。综上可知,本试验成功建立了一种快速有效诊断骆驼斯氏副柔线虫病的iELISA方法。 相似文献
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Charlier J Duchateau L Claerebout E Vercruysse J 《Preventive veterinary medicine》2005,68(2-4):277-288
An indirect Ostertagia ostertagi ELISA on milk is a promising diagnostic tool in bovine parasitology. Interpretation of the test results requires a good knowledge of the test characteristics. In this study, border effects, the repeatability of the ELISA and the effect of different factors such as storage, skimming and freeze–thaw cycles of the milk samples were investigated. The border effects trial showed that significant border effects can occur. The repeatability trial was conducted over 3 days. An alternative graphical technique to assess the repeatability over a large number of ELISA plates measured over different days was developed. From these graphs, it was obvious that the ODR values obtained on the third day were deviating from the values on the first and second day. On the third day, also abnormal control values were observed. When the control values were normal, 94% of the variability was explained by the milk sample and 6% by assay variability. The expected 95% range of the difference of 2 ODR readings of the same sample on the same plate and the same sample on different plates was −0.14 to 0.14 and −0.16 to 0.16. No extra variability was observed when samples were tested on a different day, however these results are based on the measurement of 2 days. Storage for 2–4 days at 4 °C, using whole milk instead of skimmed milk and up to 2 extra freeze–thaw cycles of the milk samples did not significantly affect the test results. 相似文献
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The sperm-bearing fractions of 163 ejaculates collected from 6 Beagles aged two years were used to test the cryoprotective effect of DMSO and glycerol in deep freezing investigations. It could be shown that the cryoprotective capacity of glycerol was better than the one of DMSO. Glycerol should rather be added to the sperms at + 5 degrees C than at + 25 degrees C. The best preservation results were achieved by a combined addition of DMSO and glycerol under the following conditions: time of cooling down to +5 degrees C of the extended sperm fractions 50 minutes, final concentration of DMSO 1% (max.), final concentration of glycerol 8% (max.), glycerol equilibration time 30 minutes. 相似文献