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1.
AIM: To study the immunogenicity and biocompatibility of xenogeneic swine corneal stroma as biological carrier for cornea reconstruction and to reconstruct corneal endothelial tissue with this carrier in vitro. METHODS: (1) The lymphocytes from the peripheral blood of F344 rats were immunologically labeled by anti-rat CD25-FITC and anti-rat CD4/CD8-PE, then determined by flow cytometry (FCM) at 12th， 90th day after intracorneal implantation with fresh and dehydrated swine corneal stroma. (2) The fresh and dehydrated grafts made of swine corneal stroma were implanted intralamellarly in corneas of New Zealand rabbits. Clinical examinations were performed monthly and histological examinations were made at 14th， 30th， 60th， 120th and 240th day. (3) The cat corneal endothelial cells were seeded on the Descemet's membrane of the dehydrated swine corneal stroma, then cultured in the medium with epidermal growth factor and laminin for 7 days. The morphology of reconstructed tissue was tested by microscope. RESULTS: (1) Compared to isograft group and negative control, the expression CD4＋CD25＋， CD8＋CD25＋ and CD4＋/CD8＋ of xenograft rat group implantation with swine corneal stroma did not appear significantly different in statistics (P>0.05). (2) In the total 12 rabbits, all the cornea grafts survived without rejection reaction， corneal haze or corneal neovascularization. The fresh grafts got transparent after 2 months, and the dehydrated grafts got transparent after 6 months. Histological study demonstrated both fresh and dehydrated stroma grafts had fused with rabbits'corneal stroma very well without lymphocytes infiltrating. (3) As shown in histological observations, the reconstructed cat corneal endothelial tissue was similar to the nature tissue. Cultured cat endothelial cells connected tightly to each other and attached to the Descemet's membrane closely. CONCLUSION: Swine corneal stroma has low immunogenicity and satisfying biocompatibility,it is an ideal biological carrier for cornea reconstruction in vitro.  相似文献   

2.
AIM: To study the change of cellular immunological function in patients with locally advanced lung cancer before and after operations. METHODS: A lung cancer group of 20 cases with locally advanced lung cancer (group A), a benign disease group of 20 cases with lung benign disease (group B) and a normal group of 20 cases from healthy volunteers (group C) were set up. The levels of the peripheral blood T lymphocyte subsets (CD+3, CD+4, CD+8, CD+4/ CD+8 ratio) were detected in the group A before operation and on the 10th day and 17th day after operation by indirect immuno-fluorescence assay and contrasted with the group B and group C. RESULTS: The levels of T lymphocyte subsets in group A were abnormal before operation, CD+3, CD+4/ CD+8 ratio were significantly lower than those in group B and group C (P<0.05), and CD+8 was significantly higher (P<0.05). CD+3 significantly increased (P<0.05) and CD+8 decreased (P<0.05) on 10th day and 17th day after operation. CD+4/ CD+8 ratio significantly increased on 17th day after operation (P<0.05). There was no significant difference of the levels of T lymphocyte subsets between the 10th day and 17th day after operation. CONCLUSIONS: The patients with locally advanced lung cancer have a remarkable impairment of immunological function, which mainly show stronger immunosuppression and have some recovery after operation. In the view of immunology, the surgical resection for locally advanced lung cancer shows active significance.  相似文献   

3.
AIM: To investigate the levels of neuropeptides and electrolytes in the patients with acute traumatic brain injury.METHODS: Seventy-eight patients with acute brain injury were divided into mild, moderate and severe groups according to their GCS scores. The serum levels of arginine vasopressin (AVP) and angiotensin II (Ang II) were measured on day 1, 3 and 7 after injury. The serum levels of electrolytes were also measured on day 1. Forty-one subjects who received healthy check-up served as normalcontrols. RESULTS: Compared with normal control group, the serum levels of AVP and Ang II significantly increased in the patients with traumatic brain injury (P<0.01), depending on the severity of brain injury. Both neuropeptides reached the peak on day 3 after injury. The concentrations of serum potassium and calcium decreased in the patients with acute brain injury(P<0.01),also showing a severity-dependent tendency. No significant change of serum sodium in the patients with brain injury was observed. CONCLUSION: The serum levels of arginine vasopressin and angiotensin II canalso be used as the severity indicators of traumatic brain injuries. Decrease in serum potassium and calcium can also be used to evaluate the severity in patients with acute traumatic brain injury.  相似文献   

4.
AIM: To observe the immunoregulation of allogeneic cornea on the human peripheral blood T lymphocytes in vitro. METHODS: After Co-culture of human peripheral blood lymphocytes and allogeneic cornea in vitro, T lymphocytes were labeled by monoclonal antibody, and analyzed by fluorescent activated cell sorter (FACS). RESULTS: CD25 expression on T lymphocytes in control was 25.2%, after stimulated by the allogeneic cornea or PDB, CD25 expression on T lymphocytes was 56.8% and 80.9%, respectively. After stimulated by the allogeneic cornea, CD25 expression on CD4+ or CD8+ T lymphocytes were 67.3% and 52.3%, respectively. CONCLUSION: Allageneic cornea stimulates CD25 expression on human peripheral blood T lymphocytes, and the CD25 expression on CD4+ T lymphocytes is more prominent than CD8+ T lymphocytes.  相似文献   

5.
AIM: To investigate differentiation of CD34+ cells in human umbilical blood into eosinophils under the condition of cell culture in vitro. METHODS: CD34+ cells were separated and purified from human umbilical blood. The cells were divided into negative group, IL-5 group and allergic rhinitis serum group. The differentiation ability of the cells was measured by flow cytometry, HE staining and electron microscope at the first day, second day, 7th day, 14th day and 28th day culture. RESULTS: The proportion of CD34+ cells in IL-5 group and allergic rhinitis serum group were decreased at the second day. The proportion in allergic rhinitis serum group was lower than that in IL-5 group significantly. The typical structure of eosinophils was observed at the second day. CONCLUSION: The allergic patient serum and IL-5 induce differentiation of CD34+ cells in human umbilical blood to eosinophils.  相似文献   

6.
AIM:To investigate the role of SDF-1α in migrating of bone marrow stromal cells to the injured areas.METHODS:Ischemic brain lesion model was created in rats by permanent middle cerebral artery occlusion (MCAO).48 SD rats were divided randomly into 2 groups.Group 1:phosphate buffered saline (PBS 1 mL) for control (n=25); Group 2:BMSCs (2×106) were injected intravenously at 24 h after MCAO (n=24).After propagated in BMSCs, Ad5/F35 GFP (green fluorescent protein) was infected to BMSCs.The expression of SDF-1α (stromal cell-derived factor-1α) mRNA in the penrumbral tissue was assayed by real-time quantitative PCR.The expression of CXCR4 on MSCs was detected by flow cytometry.Confocal microscopy was used to detect the GFP-labeled MSCs migration.RESULTS:Ad5/F35 GFP signals was observed in almost infected BMSCs.The expressions of SDF-1α mRNA in the thalamus and hippocampus of the ischemic brains were peaked at 3rd day after stroke, followed by a decrease at 14th day post-ischemia.The expression of SDF-1α mRNA in the cortex of the ischemic brains was peaked at 7th day post-ischemia, still at high level at 14th day post-ischemia.The median percentage of surface CXCR4 expression in BMSCs was 14%.GFP labeled BMSCs were detected in the origination of the middle cerebral artery (olfactory area) at 6 h, after 3 days in the prenumbra tissue such as thalamus, and in the cortex more labeled cells were found after 14 d post-ischemia.CONCLUSION:BMSCs can pass through the blood brain barrier of ischemic rats.Its mechanism might be associated with the expression of SDF-1α in the ischemic brain.  相似文献   

7.
AIM:To explore the molecular effects of Astragalus polysaccharide(AP) on improving nervous functions and preventing neuronal apoptosis in rat cerebral cortex with cerebral ischemia and reperfusion. METHODS:One hundred and twenty male Wister rats were randomly divided into sham operation group(SOG), model groups(MG-1 d, 3 d and 7 d), low-dose AP treatment groups(L-APTG-1 d, 3 d and 7 d), and high-dose AP treatment groups(H-APTG-1 d, 3 d and 7 d). The right middle cerebral artery of the rats in MG and AGTG was intercepted by operation to induce ischemic brain injury. The rats in L-APTG and H-APTG were treated with AP at the doses of 5 mg/kg and 15 mg/kg by intraperitoneal injection, respectively. On the 1st day, 3rd day and 7th day after operation, those animals were sacrificed to collect the brain specimens for the study after cerebral blood flow reperfusion and determination of neurological deficit scores. The structural changes of the neurons were observed under electron microscope. Apoptosis was analyzed by flow cytometry. The protein levels of heat-shock protein 70(HSP70), protein kinase B(PKB) and P53 in cerebral corical neurons were determined by immunohistochemical staining and Western blotting. RESULTS:The neurological deficit scores and the apoptotic rate of cerebral cortical neurons in H-APTG were significantly lower than those in MG and L-APTG(P<0.05). The structures of the neurons in H-APTG, such as ribosome endoplasmic reticulum, nucleolus, Golgi complex, mitochondria, etc, were better than those in MG and L-APTG. On the 1st day, 3rd day and 7th day, the protein levels of HSP70 and PKB in cerebral cortical neurons in H-APTG were significantly higher than those in L-APTG, which were significantly higher than those in MG(P<0.05). However, the P53 protein level in H-APTG was significantly lower than that in L-APTG, which was significantly lower than that in MG(P<0.05). CONCLUSION:AP improves nervous functions and inhibits neuronal apoptosis during ischemia and reperfusion. The molecular mechanisms are associated with variations of protein expression in cerebral cortical neurons, such as promotion of HSP70 and PKB and inhibition of P53.  相似文献   

8.
AIM: Humanized-NOD/SCID(hu-NOD/SCID) mouse model was established and the level of immune reconstitution was assessed in this model. METHODS: Mononuclear cells (MNC) and CD34+ cells were isolated or sorted from cord blood(CB). Human CD45, CD19, CD3 markers on cells from NOD/SCID murine peripheral blood(PB), bone marrow(BM), thymus were detected by FCM from 4 to 10 weeks after hematopoietic stem cell transplantation. After 10 weeks, the gene expressions of the human β2M and RAG2 were detected by RT-PCR in PB or bone marrow of mice model. RESULTS: Human CD45, CD19, CD3 cells populations in PB and BM were found by flow cytometry in mice model transplanted with CD34+ cells or CB MNC from 4 to 10 weeks. The highest positivity of human lymphocytes was at 8 week after transplantation. The levels of human cell engraftment in mice transplanted with CD34+ cells were higher than those in mice transplanted with CB MNC. The mRNA of human β2M and RAG2 were found by RT-PCR in BM.CONCLUSION: The higher level of human lymphocyte engraftment is established in NOD/SCID mouse model transplanted with CD34+ compared with CB MNC. The maturation of T lymphocytes could be happened in bone marrow of mice model.  相似文献   

9.
AIM: To observe the effects and location of autologous bone marrow stem cells (BMSCs) transplanted through renal artery into ischemic-reperfusion (I/R) injured kidney.METHODS: BMSCs were collected from rabbits after isolated and then labeled with 5-bromo-2-deoxyuridine (BrdU). Twenty-eight rabbits were subjected to clamping renal pedicles for 105 min and divided into the transplantation group and control group randomly. BrdU labeled BMSCs or saline were injected into the kidney by renal artery, respectively. Before and after I/R at the 1st, 3rd, 5th, 7th, 14th, 21th and 28th d, the venous blood was collected to measure serum Cr and BUN. In the same time, renal tissue was collected for pathological and immunohistochemical study.RESULTS: After I/R, serum Cr and BUN levels in the rabbits in two groups became higher, and on the 1st and 3rd d after I/R, reached the highest level. On the 7th d the serum Cr and BUN levels in transplantation group were lower than those in control group. On the 28th d the levels of serum Cr (90.1±11.1) μmol/L and BUN (8.0±1.5) mmol/L in transplantation group were significantly lower than those in control group (135.6±32.5) μmol/L and (10.9±2.5) mmol/L, respectively (P<0.05). Pathological observation of renal tissue showed the degeneration, necrosis and abscission in renal tubular epithelial cells. The BrdU positive staining by immunohistochemical study was found in renal tubular in transplantation group on the 5th and 7th d after I/R, and maintained to the end of experiment, but no detection in control group.CONCLUSION: BMSCs transplantation through renal artery accelerates the repairment of renal functions after acute tubular necrosis by ischemic-reperfusion. Autologous BMSCs transplantation is a safe and valid method to accelerate the repairment after acute tubular necrosis.  相似文献   

10.
AIM: To explore the impact of granulocyte colony-stimulating factor (G-CSF) on acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in a murine model and its possible mechanisms. METHODS: Male C57BL/6 (H-2b) and BALB/c (H-2d) mice were used as the allogeneic and syngeneic donor mice, respectively. Moreover, female BALB/c mice were used as recipient mice. The recipient mice were conditioned by a single dose (8 Gy) of total body irradiation (TBI). The recipient mice were randomly divided into 7 groups: TBI group, Syn-BMST control group, post-Syn-BMST G-CSF administration (Syn-BMST+G-CSF)group, allo-BMT control group, post-allo-BMT G-CSF administration (allo-BMT+G-CSF)group, allo-BMST control group and post-allo-BMST G-CSF administration (allo-BMST+G-CSF) group. The mice in control groups and G-CSF administration groups were subcutaneous injected with 0.1 mL normal saline (NS) and 0.1 mL NS containing 2 μg G-CSF per day from 1st day, respectively. The effect of G-CSF on aGVHD was evaluated by clinical manifestations and pathological changes, as well as survival time of the mice in different groups. The serum levels of IL-2, IL-4, IFN-γ and TNF-α in allo-BMST and allo-BMST+G-CSF groups were detected by ELISA at 10th day. Flow cytometry was used to analyze the immunophenotypes of splenocytes at 10th day. RESULTS: The mice in TBI group were all died for hematologic failure on 9~15 d after TBI. No effect of G-CSF on the survival of the mice underwent Syn-BMST and transplantation of single allogeneic marrow cells was observed. The mean survival days in allo-BMST group and allo-BMST+G-CSF group were (34.8±4.5) d and (19.8±6.1) d'respectively (P<0.01). Moreover, post-transplant administration of G-CSF increased the spleen total nucleated cells count (SpTNC), NK cells subset, and DC1/DC2 ratio in the spleen with over 99% of donor chimerism rate at 10th day. No difference in the levels of serum IL-2, IL-4, IFN-γ and TNF-α between the 2 group at 10th day was found. CONCLUSION: The administration of G-CSF after allo-BMST significantly aggravates mouse aGVHD. The expansion of NK cells stimulated by G-CSF may be involved in the mechanism of generating alloreactivity against host cells. These results imply there may be potential risk of evoking or aggravating acute GVHD if G-CSF is administered in the early stage of clinical allo-HSCT.  相似文献   

11.
AIM: To investigate the effects of deep-frozen treatment on the immune response of bone-patellar tendon-bone (BPB) xenograft rejection. METHODS: A muscle pocket model was used to study the immune response of rat to deep-frozen treated BPB xenograft from guinea pig, autograft and fresh xenograft served as controls. The expression of T-cell surface activation antigen CD25 in the peripheral blood and the morphological changes of the implants were used to measure the immune response. RESULTS: The expression of CD25 in CD4+, CD8+ cells greatly increased 3 d after fresh BPB xenograft, the obvious difference to that of autograft (P<0.05) was observed. It reached a peak level 14 d after fresh BPB xenograft and didnt decrease 35 d after transplantation. The expression of CD25 in CD4+, CD8+ cells was greatly inhibited in deep-frozen treated BPB xenograft. Its expression was delayed and just had a small increase 14 d after transplantation. The P values were all less than 0.05 compared to those of fresh xenograft at every time-point after transplantation. Histologic examination showed that there were a few lymphocytes surrounding the bone and tendon tissue in deep-frozen treated BPB xenograft, but the lymphocytes hadnt invaded into the tissues. No tissue necrosis, but a clear tendon structure and regular fibrogen arrangement were observed. There were large amount of fragmentary lymphocytes infiltrating into or surrounding the bone and fibrogen tissues after fresh xenograft, the bone was broken into pieces, the tendon structure was unclear and the fibrogen arrangement was irregular, quite a few of inflammatory cells such as acidophilic granulocytes and macropolycytes were found in some slices. CONCLUSION: Deep-frozen treatment markedly reduces BPB xenograft antigenicity and inhibits the immune rejection.  相似文献   

12.
AIM: To study cellular and molecular mechanism involved in increasing susceptibility of infection in psychological stress persons. METHODS: Comparative studies were performed with double staining and flow cytometry analysis on immunophenotyping and in vitro expression of early activating surface molecule CD69 in response to mitogens on T cells from peripheral blood of 20 healthy college student volunteers before and after psychological stress. A series of term final examinations was defined as psychological stress. RESULTS: Immunophenotyping analysis showed no statistically significant difference in the percentage of CD2, CD3, CD4, CD8, CD19, CD20, CD16 and CD56 positive lymphocyte populations before and after psychological stress. There was a statistically significant decrease in the in vitro expression of CD69 in response to polyclonal stimulators on the T cells from persons after psychological stress than those before psychological stress. The percentage of CD69 expression (CD69+CD3+/CD3+%) in response to PHA and PDB in the whole blood culture for 72 hours decreased respectively from 28.1±4.1 and 80.7±6.8 on the T cells obtained before psychological stress to 17.6±3.8 and 65.8±7.9 on those obtained after psychological stress, while there was no statistically significant difference between the CD69 expression rates without stimulators on the T cells obtained before and after psychological stress. CONCLUSIONS: The effects of psychological stress to immune system is not on the level of changing proportions of the sub-populations within peripheral blood lymphocytes. Psychological stress can decrease the activating response of T cells in healthy persons, which may be responsible for the increase of susceptibility to infection in the psychological stress persons.  相似文献   

13.
AIM: To purify human yolk sac mesenchymal stem cells (hYS-MSC) and investigate its osteogenic and neurogenic differentiation potentials. METHODS: hYS-MSC were separated from yolk sac and purified via passage culture. The karyotype of hYS-MSCs was analyzed via G-banded characteristics. Flow cytometric analysis was used to determine the cell cycle and phenotype of hYS-MSC. The AKP expression of hYS-MSC was also tested. Osteogenic differentiation of hYS-MSCs was induced by 10-8mol/L dexamethasone, 10 mmol/L β-glycerophosphate and 50 mg/L vitamin C. Alizarin red S stain was used for identification of mineralization. β-mecaptoethanol or salviae miltiorrhizae were used to induce neurogenic differentiation of hYS-MSCs. The expressions of NSE, NF and GFAP were identified by immunohistochemical method. RESULTS: hYS-MSCs could be purified at passages 2 or 3. The cell cycle analysis suggested that hYS-MSCs showed strong proliferational potentials by which the cells kept normal diploid karyotype during the in vitro culture. Flow cytometry showed the phenotype of purified hYS-MSCs was uniformly positive for CD29, CD44, CD105, and CD166, and negative for reactivity to antigens CD34, CD45, or CD86. hYS-MSCs were weakly but clearly positive in AKP. Osteogenic differentiation was appeared after induction of osteogenic differentiation. hYS-MSCs, which were of spindle shape, uniform in size, were induced to pleomorphism osteoblast-like cells which expressed high level of AKP. Aggregates or nodules were formed at day 7 and calcium accumulation was detected by alizarin red S staining on day 10 or day 14. Neurogenic differentiation of hYS-MSCs was induced by β-mecaptoethanol or salviae miltiorrhizae. NSE, NF or GFAP positive cells were detected by immunohistochemical staining. CONCLUSIONS: hYS-MSCs have strong proliferation potential and the normal diploid karyotype is kept during the in vitro culture. The phenotype of hYS-MSCs is coincident with adult hMSCs. hYS-MSCs could be induced to differentiate into osteogenic or neurogenic cells.  相似文献   

14.
AIM: To establish an animal model of rheumatoid arthritis(RA) in DBA/1 mice induced by immunodominant mixed peptides derived from glucose-6-phosphate isomerase(GPI). METHODS: The DBA/1 mice were immunized with emulsified mixed peptide fragments of hGPI325-339+hGPI469-483 or single peptide hGPI325-339 in complete Freund's adjuvant by subcutaneous injection to induce the model of RA. Body weight, ankle joint symptom scores, the pathological change of the ankle joint, the levels of CD4+ T cells in the spleen and peripheral blood, the proportion of iNKT cells in the peripheral blood, and the levels of TNF-α and IL-6 in serum were detected to evaluate and analyze the model. RESULTS: The hind paw of the model mice appeared red swelling on the 8th day, and then aggravated gradually to the limbs. The red swelling reached peak on the 14th day, and then relieved gradually. Inflammation response dominated by lymphocytes and monocytes was observed in the ankle joint. The inflammatory effect of mixed peptides was more obvious than that of the single one(P<0.05). Compared with control group and the mice treated with single peptide, the weight gain was slow, the amount of CD4+ T cells in the peripheral blood and spleen were increased, the proportion of peripheral iNKT cells in the inflammatory peak was decreased(P<0.05), and the serum level of TNF-α was increased significantly(P<0.05) in the mice treated with mixed peptide fragments. CONCLUSION: The immunological characteristics of RA model induced by mixed GPI peptides in DBA/1 mice is closer to that in RA patients, especially in the immunopathology of iNKT cells. Therefore, this model can be used as a new tool for studying the mechanism and immunological intervention of RA.  相似文献   

15.
AIM:This study aimed at elucidated the possibility that prevent tissue from secondary injury by regulating polymorphonuclear neutrophil (PMN) apoptosisin vitro. METHODS:Neutrophils, isolated from peripheral blood, were incubated with sodium arsenite (Ars), tumor necrosis factor (TNF-α), interleukin-6 (IL-6), burned serum and traumatic serum, respectively. Apoptosis rate, expression of CD11b, respiratory burst and concentration of Ca2+ were then measured. RESULTS:The elevation of PMN apoptosis rate was Ars concentration dependent, but activated PMN became insensitive to Ars. IL-6 delayed PMN apoptosis (compared with control at 24 h,P＜0.05), inhibited CD11b expression. Burned and traumatic serum had more significant effects on PMN compared with IL-6. CONCLUSION:PMN was observed for the first time to resist spontaneous apoptosis when activated by LPS/TNF, and became insensitive to apoptosis-inducing substance Ars. IL-6, burned serum and traumatic serum could delay PMN apoptosis and recover PMN functions partly.  相似文献   

16.
AIM: To study the possible immunologic mechanism of mesenchymal stem cells (MSCs) by which the hematopoiesis of mice with aplastic anemia (AA) is improved. METHODS: The mice model of immuno-mediated aplastic anemia was established. Thirty BALB/c mice were divided into 3 groups: radiation group, AA model group and MSCs group. The mice in radiation group only had processing of 5 Gy ［60Co］γ radiation. The mice in AA model group were intravenously injected with the cell suspension of thymocyte and lymph-node cell mixture (prepared from DBA/2 mice, 1×106 cells) after 5 Gy ［60Co］γ radiation. The animals in MSCs group received the same treatment as the mice in AA model group did, and afterwards was injected with 1×106 MSCs intravenously at 3 days. All the mice in the 3 groups were observed and analyzed the following parameters: peripheral blood cells, pathological features of bone marrow, the relation between the changes of peripheral CD4+, CD8+, CD4+/CD8+, NK cells and the hematopoiesis. RESULTS: After 5 Gy ［60Co］γ radiation, the peripheral blood cells in all groups decreased at 7 days, while at 21 days those in MSCs group and radiation group restored to normal but those in AA model group was still at lower level. At 7 days, the cells of CD4+, CD8+ and CD4+/CD8+ in each group were similar without statistical difference, but the number of NK cells in MSCs group was quite lower than that in the other 2 groups (P<0.05). At 14 days, the CD4+ cells in all groups were obviously decreased, while the CD8+ cells in MSCs group and AA model group were significantly higher than those in radiation group. At 21 days, the CD8+ and NK cells in MSCs group were quite the same as those in radiation group while those in AA model group were obviously higher compared to the other 2 groups. The number of adipose cells observed in the pathological slice of femoral bone in MSCs group and radiation group was no difference while that in AA model group was much higher. CONCLUSION: MSCs transfusion improves the hematopoiesis in mice with aplastic anemia by regulating the functions of immune cells.  相似文献   

17.
AIM：To test the effects of FTY720 on mouse intestinal allografts.METHODS：C3H mice（H-2k）were used as donor and C57BL/6 mice (H-2b) as recipients.FTY720 group,allogeneic control group and isogeneic control group were set up.6 and 14 days after transplantation,murine intestinal grafts were harvested for histologic assessment.Lymphocytes were collected from mesenteric lymph nodes (MLN),Peyer’s patch (PP),lamina propria lymphocytes (LPL) and intraepithelial lymphocytes (IEL) in the graft,then were analyzed by cytometry.RESULTS：Rejection was inhibited in FTY720 group at the 6th post-transplant day,although not at the 14th day.Recipient CD4+ and CD8+ T cells,CD19+ B cells,as well as γδ TCR lymphocytes,were greatly reduced by FTY720 therapy.The similar action of FTY720 was also revealed in Gr1+CD11b+ monocytes.CONCLUSION：FTY720 is efficient on alleviating allo-immune response by reducing the infiltration of both lymphocytes and monocytes into the graft in a mouse intestinal transplantation model.  相似文献   

18.
AIM:To investigate the effect of microwave ablation of liver cancer on the cellular immunity in mice.METHODS:A C57BL/6J mouse model of liver cancer was established by subcutaneous injection of Hepa 1 - 6 cells.The tumors were subjected to micr owave ablation under the ablation condition of 45 ℃,50 ℃,55 ℃ or 60 ℃ fo r 180 s.The CD4＋ T cells,CD8＋ T cells and natural killer cells (NK) in p eripheral blood were detected by FACS.The cytotoxicity of splenic NK and splen ic cytotoxic T lymphocytes (CTL) activated by inactivated Hepa 1-6 cells was ass ayed by LDH method.RESULTS:The proportions of CD4＋ T cells,CD8＋ T cells and NK cells in peripheral blood in 50 ℃ and 55 ℃ group at 21 d after ablation we re significantly increased and that of NK cells in 60 ℃ group was significantly in creased.There was no significant difference between those in group 42 d after ablation and control.The cytotoxicities of splenic CTL and NK cells in 50 ℃ an d 55 ℃ groups at 21 d or 42 d after ablation were significantly increased,and they were much higher than those in 45 ℃ group at the same time.The cytotoxicities of splenic CTL in 50 ℃ and 55 ℃ groups at 21 d after ablation were much highe r than that in 60 ℃ group at the same time.CONCLUSION:Under a certain ablation temperature,microwave abla tion of liver cancer promotes the cellular immunity.  相似文献   

19.
XIE Jing  GAO Hui-chun  ZHENG Xi 《园艺学报》2016,32(10):1905-1908
AIM: To investigate the effect of astragaloside IV on neurological function and the protein expression of neuron-specific enolase (NSE), Notch1 and NF-κB in acute cerebral hemorrhage (ACH) rats.METHODS: ACH model in rats was established via injection of autologous blood, and the rats were divided into 4 groups:sham, ACH, ACH+astragaloside IV (100 mg/kg) and ACH+astragaloside IV (200 mg/kg) groups. The impairment of neurological function in each group was graded, and the brain coefficient and water content were calculated. The serum level of NSE was measured by ELISA. Additionally, the expression of Notch1 and NF-κB was determined by Western blot.RESULTS: Astragaloside IV improved the neurological function and decreased the brain coefficient and water content in ACH rats. Moreover, the ACH-induced increase in NSE was inhibited after astragaloside IV treatment. Similarly, astragaloside IV also significantly attenuated the expression of Notch1 and NF-κB in ACH rats.CONCLUSION: Astragaloside IV attenuates the impairment of neurological function in ACH rats, which may be through decreasing the NSE level and down-regulating the expression of Notch1 and NF-κB.  相似文献   

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