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1.
G. Ram M. K. Bhan Ashok Ahuja S. R. Meena M. K. Kaul K. K. Gupta R. L. Jolly R. K. Khajuria 《Genetic Resources and Crop Evolution》2007,54(3):649-654
Variability studies were carried out among different accessions of Argyrolobium roseum for 12 metric traits under natural and cultivated conditions. Almost all characters showed higher values in cultivated than
natural population. Leaf breadth recorded highest CV (30.59%). Highest percentage of vitexin and D-pinitol (0.208 and 0.773%
dwb) was observed in RAR-7 and RAR-6, respectively, under cultivated conditions. Phenotypic coefficient was higher than genotypic
coefficient of variation. The association analysis revealed that root yield had positive significant correlation with 100
seed weight (r=0.839) biomass yield (r=0.601), where biomass yield had a positive significant correlation with leaf length, single pod weight. High heritability
(97.4–99.8%) coupled with moderate genetic advance ranged between (49–67%) as a percent of mean was observed for leaf breadth,
pod length, total foliage biomass yield and total root yield/plant. This suggests that direct selection for these traits is
suitable for the improvement of this crop. 相似文献
2.
Kamal K. Panda Anath B. Das Brahma B. Panda 《Genetic Resources and Crop Evolution》2009,56(5):629-637
Pandanus tectorius Parkinson (
P. fascicularis Lam.) of the family Pandanaceae constitutes one of the major bioresources of Ganjam coast, Orissa; used mainly in small scale
perfume industry for aromatic compound extracted from the male inflorescences. In order to establish genetic diversity, if
any related to perfume yield, samples of P. tectorius representing male populations from seven locations representing populations I–VII along the coastline of Orissa, India, were
analysed for somatic chromosome number, 4C genomic DNA content, randomly amplified polymorphic DNA (RAPD) as well as phytochemicals.
The somatic chromosome number in all the populations I–VII was 2n = 60. The chromosomes were of minute size without showing any remarkable structural variation. Like wise the average 4C DNA
content was 5.09 pg (≅4,912 Mbp) that showed no intra- or inter-population differences. Out of 54 decamer primers tested,
a total of 1,260 amplicons were obtained from 34 primers accounting 43.49% polymorphism. Molecular phylogenetic analysis of
the seven populations revealed two distinct branches, with populations II and III in one and the rest populations in the other
branch of the phylogenetic tree. It was important to note that the unique populations II and III confined to the Ganjam coast
of Orissa having RAPD markers: OPA 09–940 bp, OPA 09–705 bp, OPC 14–1,500 bp, OPC 14–700 bp, OPC 20–1,475 bp, OPC 20–1,350 bp,
OPC 20–920 bp and OPC 20–700 bp, were distinguished form the rest of the populations. The aforesaid populations (II and III)
are well known to produce aroma of high quality and yield, composed of primarily phenyl ethyl methyl ether (66.8–83%) and
terpinen-4-ol (5–12%) along with a number of other phyto-chemical compounds that support the flourishing perfume industry
and livelihood of the local people in the region. The findings underscored the possible role of local eco-geography in contributing
to the micro-evolution of unique high perfume yielding genotypes of P. tectorius that represented populations II and III at Ganjam coast, which were genetically distinct from the rest of the populations
revealed by RAPD analysis. 相似文献
3.
Naresh Kaushik Sushil Kumar Krishan Kumar R. S. Beniwal N. Kaushik S. Roy 《Genetic Resources and Crop Evolution》2007,54(8):1827-1832
Forty Candidate Plus Trees (CPTs) of Pongamia pinnata were selected based on the morphometric traits to identify suitable seed sources with high oil content and for production
of quality seedlings for mass afforestation in different forestry and agroforestry programmes. Significant genetic variability
and association were recorded among 40 CPTs for pod and seed traits. Maximum 100-seed weight (186.80 g) and pod-weight (403.94 g)
was recorded in CPT-33, while CPT-18 showed maximum oil content (44.07%). In general, phenotypic coefficient of variation
was higher than genotypic coefficient of variation indicating the predominant role of environment. High heritability (broad
sense) and genetic gain observed for pod–seed ratio (99.00%, 87.78%), 100-seed weight (100.00%, 66.99%) and 100-pod weight
(98.00%, 57.38%), respectively indicate additive gene action. Seed weight and pod weight showed positive and significant correlation
with oil content. CPTs 18, 20, 33, 13 and 29 were found to be the best on the basis of oil content and pod–seed characters. 相似文献
4.
An investigation was conducted to determine the extent of diversity and relationships among a worldwide safflower (Carthamus tinctorius L.) germplasm collection and to find out adapted accessions that can be used in an organic safflower breeding program in
Germany. A total of 468 accessions was studied under organic farming conditions at Kleinhohenheim experimental station during
the seasons of 2004 and 2005. All the accessions were evaluated for 12 phenotypic traits and three rated diseases. Multivariate
analyses have been used to measure the diversity in a subset of 200 accessions and 11 geographical regions. Generally, the
study showed that there was a large genetic variation within accessions. A coefficient of variation (CV%) for investigated
traits and diseases ranged from 2.9 to 91.0% with the highest CV was recorded for yield/m2, yield/plant and seeds/plant. The
most accessions that originated in Europe revealed relatively better performance compared to non-Europeans. High yielding,
early maturing, and disease tolerant accessions were identified. However, the low oil content (8.7–22.8%) is the primary concern
in this germplasm collection. The degree of heritability varied between 10% for lodging to 86% for plant height. Genotypic
coefficient of correlation (r
g) was slightly higher for many traits than the respective phenotypic coefficient. Oil content and seed yield/m2 were highly
significantly correlated (r
g = 0.78). The genotypic coefficient of correlation showed that selection for seeds/plant and thousand kernel weight was effective
for improvement of seed yield and oil content. The results of the principal component analysis and the clustering pattern
of accessions were consistent with the results of analysis of variance. About 78% of the total phenotypic diversity in the
germplasm was explained on the basis of four principal components and 88% of the total variation among geographical regions
was contributed by the first three principal components. The distribution of the accessions within clusters has no apparent
relationship with the geographical origin. However, many of the European accessions have a tendency to stay together. 相似文献
5.
Pedro A. López Mark P. Widrlechner Philipp W. Simon Satish Rai Terri D. Boylston Terry A. Isbell Theodore B. Bailey Candice A. Gardner Lester A. Wilson 《Genetic Resources and Crop Evolution》2008,55(2):247-275
Our goals for this research were to elucidate phenotypic and biochemical diversity in coriander (Coriandrum sativum L.) populations maintained at the North Central Regional Plant Introduction Station in Ames, IA, and examine relationships
between amplified fragment length polymorphism (AFLP) markers and patterns of phenotypic and biochemical diversity. Phenotypic
and biochemical traits were evaluated, and analyses of variance and mean comparisons were performed on the resulting data
sets. Euclidean distances from phenotypic (PD) and biochemical (BD) data were estimated, and modified Rogers’ distances (RD)
were estimated for 80 polymorphic AFLP markers. These data were subjected to cluster analyses (CA) and principal components
analyses (PCA), to reveal patterns among populations, and to analyses of molecular variance (AMOVA) for grouping patterns
from PD and BD by using the 80 polymorphic AFLP markers. Resulting phenotypic, biochemical, and molecular distance matrices
were also compared by applying Mantel tests. Our results describe significant differences among populations for all the phenotypic
traits, and dendrograms obtained from PD and BD revealed complex phenetic patterns, as did groups from PCA. The primary seed
essential oils and nearly all fatty-acid components were identified and their abundance measured; the primary chemical constituents
of corresponding PCA groups are described herein. Molecular evidence supported phenotypic and biochemical subgroups. However,
variation attributed among subgroups and groups was very low (∼4–6%), while variation among populations within groups was
intermediate (∼24–26%), and that within populations was large (∼69–70%), reflecting weak differentiation among subgroups and
groups, which was confirmed by values for fixation indices. Phenotypic subgroups described in this study differed somewhat
from previous infraspecific classifications. Weak correlations were found between the phenotypic and biochemical matrices
and between the biochemical and AFLP matrices. No correlation was found between the phenotypic and AFLP matrices. These results
may be related to coriander’s phenotypic plasticity, its wide range in lifecycle duration, its predominantly allogamous reproductive
biology, a human-selection process focused on special traits that may be controlled by few genes, and the widespread trade
of coriander seeds as a spice, which may result in dynamic, poorly differentiated molecular variation, even when phenotypic
and biochemical differentiation is easily documented.
The U. S. Government’s right to retain a non-exclusive, royalty-free license in and to any copyright is acknowledged. 相似文献
6.
Miao-Miao Li Yu-Liang Cai Zeng-Qiang Qian Gui-Fang Zhao 《Genetic Resources and Crop Evolution》2009,56(4):455-464
In this study, the genetic diversity and differentiation of 10 natural Prunus pseudocerasus Lindl. populations were investigated using inter-simple sequence repeat (ISSR) markers. Totally, 18 selected primers generated
150 loci, with an average of 8.33 bands per primer. The results showed that the percentage of polymorphic bands (PPB) was pretty low at the population level (PPB = 1.13–32%), but relatively high at the species level (PPB = 84%). Besides, a high level of genetic differentiation among populations was detected based on the gene differentiation
coefficient (G
ST = 0.7118) and the hierarchical analysis of molecular variance (AMOVA) (Φ
ST = 64.53%, P < 0.001), in line with the low inter-population gene flow (N
m = 0.2025). Moreover, Mantel test revealed a significant correlation between genetic and geographic distances among the populations
(r = 0.5272, P < 0.005). The high level of intraspecific genetic diversity was probably related with its life history traits, while its
small population size and the resultant high levels of genetic drift and inbreeding might explain the low genetic diversity
within populations. The relatively high inter-population genetic differentiation was largely attributed to its small population
size, habitat fragmentation, the mode of pollen and seed dispersal, and geographic isolation. Based on the present study,
conservation strategies were proposed to preserve this valuable natural germplasm resource. 相似文献
7.
Wheat landrace populations, collected from different altitudes of three regions of Turkey, were evaluated for variation within
and among populations. Experimental material consisted of 380 accessions, from nine provinces, in Central Anatolian Plateau
and North Transitions. The material was grown at Haymana-Ankara Research Farm of Central Research Institute for Field Crops
in 1999–2000 planting season, in a three-meter, two-row-plot trial with three bread and three durum wheat checks. Populations
were evaluated for plant height, tillers number, spike length, grains per spike, awn length, thousand kernels weight (TKW),
winter survival, and days to flowering. Observations and measurements were performed on five randomly selected plants in each
plots. Mean, coefficient of variation (CV), and range were computed for populations from five altitude intervals of 0–399,
400–799, 800–1199, 1200–1599, and 1600–1999 m, and geographical regions. Correlation, principal component (CPC) and cluster
analyses were performed later. The highest variation was recorded for awn length and the lowest for number of days to flowering.
First three principal components (PCs) accounted for 60.69% of the total variation. Cluster analysis for bread wheat, durum
wheat, cultivated einkorn and cultivated emmer grouped the species meaningfully. 相似文献
8.
Takanori Ohsako Takafumi Ohgushi Hino Motosugi Kouzou Oka 《Genetic Resources and Crop Evolution》2008,55(7):1047-1053
Genetic variability within and among eight landrace populations of tea (Camellia sinensis (L.) O. Kuntze) located in southern Kyoto, Japan, was surveyed with six microsatellite markers. The average number of alleles
per locus was 3.83 to 4.67 for landrace populations, whereas the corresponding value among modern cultivars and breeding lines
was 6.63. Expected heterozygosity values averaged over loci within landrace populations ranged from 0.498 to 0.723. A similar
level of variation, 0.682, was observed for cultivars and breeding lines. High fixation index values (0.177–0.417) for each
population are consistent with biparental inbreeding within the population. Genetic differentiation among local populations
was extremely low with F
ST = 0.062, although AMOVA revealed significant differentiation among landrace populations. We propose that these populations
share a common ancestral gene pool and that some degree of artificial selection within each population has been performed
by local farmers. Neighbor-joining analysis revealed that genetic relationships among populations reflect geographical location
of populations. This might result from more frequent genetic exchange by nearby farmers. 相似文献
9.
V. B. Sreekumar A. M. Binoy Sarah T. George 《Genetic Resources and Crop Evolution》2007,54(8):1659-1665
Artocarpus altilis (Park.) Fosberg is an important fruit vegetable tree grown in the homesteads of southern part of India. To provide reasoned
scientific management practices and conservation measures, the pattern of morphological and genetic variation were investigated
for six populations using Amplified Fragment Length Polymorphism (AFLP) markers and 15 morphological traits. The use of five
selective primer combinations on 60 samples resulted a total of 414 bands in which 85% were polymorphic. The values of Nei’s
genetic distance varied from 0.0044 (Palai–Palghat) to 0.3376 (Palghat–Mangalore). Analysis of molecular variance revealed
most of the variation within populations (57.45%) than (42.55%) among populations. The genetic variation by AFLP data is not
reflected in quantitative morphological variables. However, the genetic and geographical distances were positively correlated
which were further well supported by the PCO analysis and Dollo-parsimony tree, both show the tendency of the individuals
to group according to the geographical localities. 相似文献
10.
Pan Dong Yu-Ming Wei Guo-Yue Chen Wei Li Eviatar Nevo You-Liang Zheng 《Genetic Resources and Crop Evolution》2009,56(1):121-136
Using the 8 specific primer pairs based on the conserved motifs of plant resistance genes, the plant disease resistance gene
analog polymorphisms (RGAPs) in 15 wild emmer wheat (Triticum dicoccoides) populations from Israel had been detected. High genetic variations at the RGAP loci were observed in T. dicoccoides populations. A total of 254 discernible bands were obtained among 115 accessions, and 192 bands (75.6%) were polymorphic.
Each genotype had a unique banding profile, and the genetic similarity coefficient ranged from 0.094 to 0.862. In T. dicoccoides, the proportion of polymorphic loci (P), the genetic diversity (He) and Shannon’s information index were 0.756, 0.362 and 0.541, respectively. The proportion of polymorphic loci (P) per population averaged 0.732 (range: 0.515–0.932); genetic diversity (He) averaged 0.271 (range: 0.212–0.338); and Shannon’s information index averaged 0.404 (range: 0.310–0.493). The coefficients
of genetic distance (D) among populations averaged 0.107 (range: 0.043–0.178), and the results of Mantel test (r = 0.168, P = 0.091) showed that the estimates of genetic distance were geographically independent. Neighbor-joining cluster analysis
suggested that the genetic relationships of T. dicoccoides populations were associated with their ecogeographic distribution. The hierarchical analysis of molecular variance (AMOVA)
and the coefficient of gene differentiation (G
ST
) values revealed that most of the variations were presented within populations, although significant differences among populations
and regions were also detected. The values of P and Shannon’s information index were negatively correlated with the two factors: Tdd (day–night temperature difference) and
Ev (mean annual evaporation), whereas they were positively correlated with one water factor: Rn (mean annual rainfall). The
correlation matrix between He in the RGAPs and geographic variables contained 20 significant (P < 0.05) correlations. The present study established that T. dicoccoides in Israel had a considerable amount of genetic variations at RGAP loci at least partly correlated with ecological factors. 相似文献
11.
The study aimed at exploring durum wheat landraces to be utilized in breeding programs. 566 single durum wheat plants selected
from 117 populations collected from 12 provinces were studied. The selected material was planted for characterizing their
some qualitative and quantitative traits such as percent vitreousness, pearling index, grain protein content, seed yield and
thousand kernel weight; as well as determining time frame for germination-tillering, germination-shooting, germination-heading,
germination-maturity, tillering-shooting (T-S), tillering-heading (T-H), tillering-maturity, shooting-heading, shooting-maturity,
and heading-maturity. Mean, coefficient of variation, and confidence intervals (0.95) were computed for each of 12 provinces,
for altitudinal origins with 200 m of ranges and, for each of two geographical regions separately. The highest variation existed
for number of days between T-H and the lowest for number of days between T-S. The highest variation within developmental stages
was observed in samples from Diyarbakir with a CV of 32.96%, from 600 to 799 altitude range with a CV of 18.86%, and from
Southeast Anatolia with a CV of 20.12%. 相似文献
12.
Random amplified polymorphic DNA markers were used to study sub-structure and genetic differentiation amongst 31 populations
(seven cultivated and 24 wild populations) belonging to 14 Asiatic Vigna species. Ten pre-selected RAPD primers generated 152 polymorphic amplification products. Estimates of polymorphism indices
were higher for the wild taxa in comparison to the cultivated forms. FST values between populations ranged from 0.111 to 0.801 and Nei’s genetic diversity values between and within species varied
from 0.26 to 0.70 and 0.04 to 0.56 respectively. The high FST and FCT values indicated strong subdivision of populations and high differentiation among species. Analysis of molecular variance
was performed by grouping the populations conforming to specific species. AMOVA was also performed separately to better resolve
the differentiation of species within mungo–radiata complex. Molecular phylogenetic relationships amongst the species of radiata–mungo complex; namely, black gram (V. mungo (L.) Hepper), green gram (V. radiata (L.) Wilczek), V. radiata var. sublobata, V. radiata var. setulosa, V. mungo var. silvestris and V. hainiana, were studied through cluster analyses. Two distinct groups were recognized within the complex, with population samples of
V. hainiana forming one cluster. Further, V. hainiana appeared to be equidistant to both V. radiata and V. mungo. 相似文献
13.
David M. Cole Timothy L. White P. K. R. Nair 《Genetic Resources and Crop Evolution》2007,54(1):189-204
Knowledge of the effects of farmer practices on population genetic parameters of peach palm (Bactris gasipaes Kunth) is relevant to the improvement and conservation of the palm’s genetic resources. Microsatellite markers were used
to assess genetic diversity and population structure of peach palm in swidden-fallow agroforestry systems in northeastern
Peru. The study covered eight communities, comprising two study areas 160 km apart – one occupied by indigenous Amerindians
and the other by mixed race campesinos. Simultaneous analysis of an ex situ peach palm germplasm collection provided a means to compare population genetic parameters. Farmers who were surveyed on seed
selection practices for peach palm reported that an average of only four palms (4.3 for campesino and 1.5 for indigenous populations)
were used to provide seed for the establishment of the forest gardens sampled. As expected, inbreeding coefficients observed
within communities were relatively high (f = 0.105 − 0.210), however, observed heterozygosities within communities were also high (0.625–0.741). A metapopulation approach
was used to describe migration within and among regions, implying a hierarchical structure of gene flow which maintains relatively
high levels of genetic diversity. Seed migration was found to occur over longer distances (≤600 km) and at a higher frequency
(46% of palms sampled) in the indigenous study area, and a proportionally greater number of alleles was found (49 vs. 43 over
three loci) with twice as many private alleles occurring only in the indigenous populations. The farmers’ practice of preserving
remnant palms through successive swidden generations may have contributed to the maintenance of alleles by reducing the severity
of founder effects. Although the campesino study area exhibited a significant (20% of the variation; p < 0.01) isolation-by-distance relationship across 35 km distance, in general, both study populations had relatively limited
genetic structure (θ = 0.012–0.03), which is believed to have resulted from the exchange of seeds over long distances and
periods of time. 相似文献
14.
Phenotypic diversity was assessed for quantitative and qualitative traits in a salt-tolerant subset of the international safflower
(Carthamus tinctorius L.) germplasm collection originating from 11 countries in three regions (Central Asia, Southwest Asia and Africa) of the
Middle East. Phenotypically, the germplasm, among and within regions, was highly variable, especially for rosette- and yield-related
traits. Frequency of desirable variants of seven agronomically important traits ranged from 14% for long rosette period to
50% for no or few spines. Level of population differentiation was high for number of capitula per plant (30%), whereas most
traits partitioned their diversity (82–87%) within populations. Region-specific nonrandom associations among sets of qualitative
traits and the existence of broad morphological and phenotypic diversity in this germplasm were supported by the large number
of log-linear models needed to describe qualitative trait associations, the high number of principal components needed to
account for total variability, and the low discriminatory power of phenotypic traits among germplasm from regions and countries
in the Middle East. These results suggest that adaptation of the species to the wide spatial and temporal variation in the
Middle East resulted in a multitude of ecotypes and in enormous amount of local variation. A multivariate selection criterion
for high biological and seed yield, long rosette period and no or few spines identified five accessions from Southwest Asia
that can be introduced into subsistence farming systems as a multipurpose crop under saline agriculture. 相似文献
15.
A total of 4,840 entries of tetraploid wheat germplasm collections representing 121 accessions from Ethiopia were evaluated
for phenologic and qualitative trait diversity. The objectives were to assess the diversity patterns of the germplasm with
respect to regions, species and altitudinal class. High values of Shannon–Weaver Diversity Index (H′) were recorded for most
traits in each region, altitudinal classes, and species. Monomorphism was also high at accession levels. Both H′ and Nei’s
gene diversity value for the entire data set (total gene diversity H
T = 0.572; the within accessions gene diversity H
S = 0.112; and gene diversity among accessions D
ST = 0.460) showed the variation for the trait is mainly among accessions/populations rather than within accessions/population.
The least mean H′ value over all the traits used for the study was obtained from released varieties (among the origin groups)
and Triticum
dicoccon (among species). Triticum
durum exhibit the highest H′ for a number of traits. Accessions collected from altitudinal class III (2401–2800 m a.s.l.) and class
II (2001–2400 m a.s.l.) showed the highest H′ values for a valuable number of traits. Thus classifications using various phenology
and qualitative traits enable to identify adaptation of a genotype and would improve the evaluation of genotype for potential
adaptation. 相似文献
16.
17.
H. Sanou N. Picard P. N. Lovett M. Dembélé A. Korbo D. Diarisso J. -M. Bouvet 《Genetic Resources and Crop Evolution》2006,53(1):145-161
Forty-one shea tree populations were sampled, spanning the main climatic zones of Vitellaria paradoxa Gaertn. in Mali and 10–35 adult trees were chosen randomly in the agroforestry parklands of each area. A total of 12 morphological
traits, related to tree morphology, fruit size and leaf form were measured. The variance components showed that variation
among populations represented the smaller percentage of the total variation with most of the values varying between 15 and
30%. The repeatability coefficient was generally high for tree within populations with values ranging between 0.23 and 0.78.
Although genetic correlations cannot be accurately estimated, due to difficulties in separation from environmental effects,
the results indicate that there is a very low genetic relation between the three kinds of traits, i.e., between those related
to tree, those related to leaf and those related to fruit. Leaf and fruit size traits were positively and significantly correlated
with rainfall, although tree circumference was negatively correlated with rainfall and the significantly larger shea trees
were noted in the drier areas – an observation thought linked to human management of the parklands. Soil drainage and parkland
density, however, did not explain differences between populations for fruit traits. This study offers preliminary information
for the development of a breeding population for a shea tree improvement programme. The value of repeatability, the low correlation
between sets of traits and the distribution of variation, suggest that selection of many individual trees within a few populations,
would allow capture of large genetic gain especially for fruit traits. 相似文献
18.
Héla El Ferchichi Ouarda David J. Walker Mohamed Larbi Khouja Enrique Correal 《Genetic Resources and Crop Evolution》2009,56(7):1001-1010
Acacia tortilis (Forsk.) Hayne ssp. raddiana (Savi) Brenan (Family Fabaceae; subfamily Mimosoideae) is a pioneer tree of dry areas. In spite of its several uses, there
are no any published studies which deal with genetic diversity of Acacia tortilis (Forsk.) Hayne ssp. raddiana (Savi) Brenan in Tunisia. For this reason, here we study its genetic diversity with morphometric (only in Tunisian populations),
chromosome counting, and cytometric (in Tunisian and Ziambabwean populations) approaches. Morphological field studies of four
Tunisian populations (Bouhedma, Haddej, Hajeb and Gtar, respectively, designated as A, B, C, D) of A. tortilis (Forsk.) Hayne ssp. raddiana (Savi) Brenan using a general linear model (ANOVA) revealed statistically highly-significant differences between populations
for all examined characters (P < 0.0001). Significant differences were found also between genotypes for the majority of morphological traits (P < 0.0001) except the width of 10 pods (laP), weight of 10 pods (Wt) and seed number of 10 pods (Sn) (P ≥ 0.110). The variability in the tree, pod and seed characters can be exploited for agro-industrial purposes. The DNA amount
and chromosome numbers of populations Umguza and Gwaii River from Ziambabwe and of Tunisian populations A, B, C and D were
determined. Tunisian populations had 2C nuclear DNA contents of 2.95–3.03 pg, and were shown to be tetraploid (2n = 4x = 52), whilst the two Ziambabwean populations had 1.39–1.40 pg and were diploid (2n = 2x = 26). It is suggested that the Tunisian populations are paleotetraploids, adapted to the more-arid local conditions. 相似文献
19.
Demissew Sertse Oliver Gailing Nicolas-George Eliades Reiner Finkeldey 《Genetic Resources and Crop Evolution》2011,58(6):849-859
Juniperus procera is economically highly important but threatened tree species. It is the only species among 67 taxa in the genus Juniperus that naturally grows in Africa and south of the equator extending up to 18°S in Zimbabwe. Ethiopia is assumed to host the
largest J. procera populations, which are also believed to have high genetic variation owing to their wide ecological amplitude. This study
assessed genetic variation at AFLPs of J. procera populations in the Ethiopian highlands. In the study six populations, namely Chilimo, Goba, Menagesha-Suba, Wef-Washa, Yabelo
and Ziquala were included. A total of 20–24 trees from each population were investigated based on 128 AFLP band positions.
AMOVA revealed that most of the variation (94%) resided within populations of J. procera suggesting extensive gene flow among populations which is attributable to the outcrossing mating system and effective gene
transport mechanisms of the species. However, genetic differentiation among populations was still significant (P < 0.05), and the differentiation was significantly (P < 0.05) correlated with geographic distance. All population pairs were significantly (P < 0.05) differentiated except for Menagesha-Suba and Wef-Washa. These two populations also showed the highest gene diversity
(H
j = 0.301 and H
j = 0.297, respectively). These results are in accordance with historical records that claim the establishment of the Menagesha-Suba
juniper population as plantation of seedlings from Wef-Washa back in fifteenth century. 相似文献
20.
Hari D. Upadhyaya K. N. Reddy C. L. L. Gowda S. N. Silim 《Genetic Resources and Crop Evolution》2007,54(8):1787-1795
Pigeonpea germplasm accessions collected from low (<500 m), medium (501–1000 m), high (1001–1500 m) and very high elevation
zones (>1500 m) of Kenya were evaluated for 15 agronomic traits and seed protein content at ICRISAT, Patancheru, India. There
were significant differences (P < 0.001) among elevation zones for the number of primary and secondary branches, days to 75% maturity, pod length, seeds per
pod, 100-seed weight and seed yield. Mean values indicated that the accessions from low elevation zone were significantly
different from those collected in higher elevation zones for early flowering and maturity, number of primary branches, pod
length, number of pods per plant, seeds per pod, 100-seed weight, seed yield and harvest index. None of the accessions collected
in Kenya belonged to extra early (<80 days to 50% flowering) and early (80–100 days to 50% flowering) maturity groups, as
defined by time to flowering at Patancheru, India. Mean diversity index based on all characters indicated that accessions
from the low elevation zone are more diverse than those from the higher elevation zones. Frequency distribution for trait
extremes indicated that the accessions from the low elevation zone were early to flower and mature, short statured, produced
more primary and secondary branches with high pod bearing length, long pods, more pods per plant, more seeds per pod, a high
seed yield and harvest index. Accessions from the very high elevation zone were late flowering, with a large number of tertiary
branches, large seeds and a high shelling percentage and could be a source for cold tolerance and the breeding of vegetable
types. Results suggest that the elevation of collection sites is therefore a very important determinant of variation patterns
of pigeonpea in Kenya. 相似文献