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1.
Molecular markers based on single nucleotide polymorphisms (SNPs) are abundant and evenly distributed in a whole genome enough
to distinguish individuals in a population. In recent years, sets of SNP markers have been designed and applied for cultivar
identification in various crop species. This paper is the first to report the development of a panel of SNP markers for variety
identification in peppers. We used conserved ortholog set II (COSII) markers developed from conserved unigenes between tomato
and Arabidopsis to identify SNPs in peppers. We tested 438 COSII primer sets amplified as single PCR products out of a total 600 COSII primer
sets. Among the 438 COSII primers, 170 primer sets (38.8%) showed polymorphisms between Capsicum annuum ‘RNaky (RN)’and C. chinense ‘PI 159234 (234)’. In contrast, only 48 primer sets (11.0%) out of 438 primers sets were polymorphic between C. annuum ‘Perennial (PER), and ‘Dempsey (DEMP)’. The average frequency of SNPs plus InDels between C. annuum and C. chinense was 1/189 bp and between C. annuum spp. was 1/948 bp. Primer sets showing SNP between C. annuum PER and DEMP were re-designed to Allele Specific PCR (AS-PCR) primers and we finally selected a total of 40 SNP markers for
cultivar identification. As the result, we were able to discriminate 97.5% of the 81 commercial hot cultivars and 100% of
the 17 sweet pepper cultivars. We conclude the paper by discussing the use of the SNP marker set for cultivar identification
and other applications. 相似文献
2.
Increasing the amount of oleic acid in soybean oil would reduce the need for hydrogenation, a process that creates unhealthy
trans fatty acids. A previous study mapped six oleic acid quantitative trait loci (QTLs) from N00-3350 (~550 g kg−1 oleic acid) on soybean chromosomes (Gm) 5, 17, 18, and 19, which corresponds to linkage groups (LG) A1, D2, G, and L, respectively. The objectives of this study
were to develop high throughput assays to detect single nucleotide polymorphism (SNP) markers associated with these oleic
acid QTLs. Several candidate genes that are putatively responsible for the oleic acid phenotype in N00-3350 were identified
by BLAST searches against the soybean genomic assembly using the sequences of fatty acid synthesis genes from soybean and
Arabidopsis. Putative accC-2 and KAS I genes were located in the region of the cqOle-001 on Gm05 (LG-A1). A putative KAS I gene was located in the region of the cqOle-003 on Gm18 (LG-G) and a putative accB-1 gene was located in the region of the cqOle-004 on Gm18 (LG-G). These fatty acid candidate genes and sequence-tagged sites were used to identify SNPs between N00-3350 and ‘Boggs’.
Based on these SNPs, seven SimpleProbe probes for melting curve analysis were developed to rapidly identify the alleles from
the mid-oleic acid soybean line N00-3350 at the desired loci. These assays provide a high-throughput and rapid SNP genotyping
method which can be used to accelerate the development of mid-oleic acid content soybean cultivars. 相似文献
3.
大豆EST-SNP的挖掘、鉴定及其CAPS标记的开发 总被引：4，自引：0，他引：4
采用生物信息学方法将大豆EST序列联配到大豆基因组序列上,挖掘到大豆EST-SNP位点537个。对其靶向基因进行功能注释分析,发现他们主要参与亚细胞定位、蛋白质结合与催化以及代谢等与大豆重要农艺性状形成相关的生物过程。同时开发了简便易行的SNP检测方法,利用EMBOSS软件筛选导致酶切位点改变的EST-SNP,分别以大豆绥农14、合丰25、Acher、Evans、Peking、PI209332、固新野生大豆、科丰1号、南农1138-2的DNA及其混合的DNA为模板,设计引物进行PCR扩增,发现44个PCR产物中有36个测序峰图在预期的EST-SNP位点表现出多态性。酶切分析发现26个PCR产物具有酶切多态性,可以作为CAPS标记。结果表明该EST-SNP挖掘体系及其CAPS标记转化系统具有高效率、低成本等优点,有利于促进大豆的遗传育种研究。 相似文献
4.
5.
Interspecific hybridization of various tuberous Begonia species hybrids with Begonia socotrana results in so-called ‘Elatior’-begonias hybrids (B. × hiemalis Fotsch). In our study, genomic in situ hybridization (GISH) has been employed to assess the genome composition in eleven
‘Elatior’-begonias hybrids and their ancestor genotypes. Genomic DNA of tuberous Begonia was sonicated to 1–10-kb fragments,
labelled by nick translation with digoxigenin-11-dUTP and used as a probe whereas B. socotrana DNA was autoclaved to 100 bp fragments and used as block. The genome of tuberous Begonia was clearly pronounced in ‘Elatior’-begonias
when the probe concentration was ~3.75 ng/μl (150 ng/slide), with 30 times the excess of B. socotrana blocking DNA and stringency of post hybridization washings at 73% (0.1× SSC at 42°C). In ‘Elatior’-begonias hybrids GISH
distinguished two groups comprising short (0.6–1.03 μm in length) and relatively longer chromosomes (1.87–3.88 μm) which represent
B. socotrana and tuberous Begonia genomes, respectively. The number of chromosomes derived from tuberous Begonia ranged from 14 to 56
and for B. socotrana from 7 to 28 which suggest the presence of different ploidy levels in analyzed ‘Elatior’-begonia hybrids. Intergenomic recombination
has not been detected through GISH in hybrids analyzed. Genomic in situ hybridization turned out to be useful to identify
the genome constitution of ‘Elatior’-begonia hybrids and thus gain an insight into the origins of these cultivars. This knowledge
on the ploidy level and genome composition is essential for further progress in breeding Begonias. 相似文献
6.
Rosa Karla Nogueira Pestanana Edson Perito Amorim Cláudia Fortes Ferreira Vanusia Batista de Oliveira Amorim Larissa Santos Oliveira Carlos Alberto da Silva Ledo Sebastião de Oliveira e Silva 《Euphytica》2011,178(2):151-158
Bananas are tropical fruits grown worldwide playing a key role in market trade and especially used as main food source for
low income populations. In Brazil, bananas are mainly consumed in natura, occupying the second largest internal market. Nevertheless, this crop presents low availability of productive commercial
varieties with good agronomic characteristics. A strategy undertaken to solve this problem is the development of new cultivars
through conventional genetic breeding methods. However, this strategy presents some obstacles such as female sterility and
low number of seeds. In order to overcome these shortcomings, use of mutation induction aiming the selection of mutants with
desirable agronomic characteristics seems to have great potential for developing new cultivars. The objective of the present
work was to evaluate the genetic variability in putative banana ‘Pacovan’ (AAB genome, subgroup Prata Type) mutants submitted
to gamma ray irradiation, using a set of agronomical and molecular data (ISSR markers). The distance between the putative
‘Pacovan’ mutants varied from 0.26 to 0.64 with cophenetic correlation coefficient of 0.7669. Four mutants were selected based
on best agronomical characteristics and height. This data also shows that there is variability that can be explored after
the irradiation of ‘Pacovan’ banana mutants, which can be used in the genetic breeding program of banana aiming to develop
short new varieties that also present good agronomic characteristics. This is the first attempt to use combined data in order
to evaluate the genetic variability in putative banana mutants. 相似文献
7.
Analysis of introgression of the Tulipa fosteriana genome into Tulipa gesneriana using GISH and FISH
Southern hybridization and genomic in situ hybridization (GISH) have demonstrated that ‘Purissima’ (2n = 2x = 24) is an interspecific hybrid comprised of one genome of Tulipa (T.) gesneriana and one genome of T. fosteriana. Backcrossing T. gesneriana with ‘Purissima’ was partially successful. Simultaneous GISH and fluorescence in situ hybridization (FISH) distinguished
chromosomes from both parent genomes, as well as recombinant chromosomes, in interspecific hybrids and their progeny. Chromosome
recombination was observed in all cultivars except ‘Purissima’ and ‘Kouki’ (2n = 3x = 36). ‘Kouki’ (2n = 3x = 36) had two genomes of the T. gesneriana and a single genome of the T. fosteriana. The number of nonrecombinant T. fosteriana chromosomes in ‘Judith Leyster’ (2n = 4x = 48) and ‘Purissima’ progeny varied from two in ‘Hatsuzakura’ to six in ‘Kikomachi’ and ‘Momotaro’. The number and type
of recombinant chromosomes also differed among cultivars. The total number of translocations ranged from one in ‘Kikomachi’
to six in ‘Hatsuzakura’. Each was a combination of a single T. fosteriana fragment and a single T. gesneriana fragment, indicating that they resulted from a single crossover event. Sequential GISH and FISH analysis with rDNA probes
yielded chromosome-specific markers that were used to identify most of the chromosomes in ‘Purissima’ progeny. This is the
first report of introgression of T. fosteriana chromatin into the T. gesneriana genome. 相似文献
8.
Sarika Mathure Asif Shaikh N. Renuka Kantilal Wakte Narendra Jawali Ratnakar Thengane Altafhusain Nadaf 《Euphytica》2011,179(2):237-246
Eighty-eight aromatic cultivars collected from Maharashtra state were assessed for determinants of kernel quality (kernel
size-shape, test weight and aroma) and grain morphology such as awning, lemma and palea characters, pubescence, colour of
sterile lemma and apiculus colour. We, report seven cultivars—‘Girga’, ‘Kothmirsal’, ‘Kala bhat’, ‘Chimansal’, ‘Jiri’, ‘Kalsal’
‘Velchi’ and ‘Kali kumud’ as indigenous to southern India. Of the 69 cultivars characterized for agronomic traits 36 cultivars
were exquisite genotypes and possessed one or more superior traits such as early flowering, dwarf stature, higher number of
productive tiller per plant; long panicles; higher number of filled grains per panicle and strong aroma. Variability in aromatic
cultivars was assessed on the basis of nine traits placed aromatic rice cultivars in five clusters. Number of cultivars in
each cluster ranged from 1 to 33. 27 significant correlations were obtained in the physical, agronomic and grain morphology
traits. Aroma was found to be negatively associated with days to 50% flowering as well as with filled grains per panicle.
However, correlation between panicle length and effective tillers with aroma was not observed. Therefore, to increase the
yield, improvement in length of panicle and increasing number of productive tillers in medium or mild scented cultivars would
be the best strategy. 相似文献
9.
Shigeki Moriya Hiroshi Iwanami Kazuma Okada Toshiya Yamamoto Kazuyuki Abe 《Euphytica》2011,177(1):135-150
Differentiation of cultivars with simple sequence repeat (SSR) markers is a very useful technique for the true-to-type characterization
of cultivars and clarification of parent-offspring relationships. We developed an SSR marker set for cultivar identification
comprising 15 markers that were screened from 46 previously published SSRs. This marker set could be used for apple varieties
including Malus × domestica and/or other Malus species. These SSRs successfully characterized 95 apples, including the leading and major founding cultivars used worldwide
for modern apple breeding. Therefore, this marker set could be applied to almost all apple cultivars. We also analyzed the
parent-offspring relationships of 69 cultivars by considering allele transmissions. This analysis revealed the true parentage
of the following seven cultivars: ‘Kizashi’, ‘Chinatsu’, ‘Honey Queen’, ‘Haruka’, ‘Seirin’, ‘Ozenokurenai’, and Morioka #48.
This analysis also revealed a parentage discrepancy for ‘Hacnine’. From the parent-offspring analysis, two microsatellite
mutation events at alleles inherited from pollen parents were observed. 相似文献
10.
Optimal carbamate treatment conditions were studied for flower induction in seedlings of an asparagus cultivar ‘Mary Washington
500 W’. Flower induction was most accelerated by soaking seeds in 50 mg l−1 carbamate solution for 12 days at 25 °C under the fluorescent light. Longer exposure to carbamate over a 12 day period induced a higher percentage of seedlings
to flower. A higher percentage of flowering seedlings were male. Flower induction frequency among seven cultivars through
carbamate treatment widely ranged from 13 to 67%. ‘Geynlim’, ‘Mary Washington 500 W’ and ‘Welcome’ exhibited a high percentage
of flowering seedlings, while ‘Larac’ and ‘Vulkan’ showed low values. Only male flowers were induced in all-male cultivars.
Variation in pollen germination was found within all cultivars. ‘Geynlim’, ‘Cito’ and ‘Mary Washington 500 W’ showed high
values of average pollen germination. Application of carbamate compound to induce flower production can rapidly produce homogenic
cultivars which include both sexes. This is necessary for genetic studies and breeding purposes.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
11.
Genotypes for the glume colour character have been studied in 27 cultivars of common wheat (Triticum aestivum L.) originated
from old landraces, and 1 specimen of T. petropavlovskyi Udacz. et Migusch. by means of analysis of the F2 populations. The following tester lines have been used: white-glumed ‘Novosibirskaya 67’ ‘Diamant I’, and ‘Federation’, carrying
the Rg1 gene alone; lines RL5405 and near-isogenic ‘Saratovskaya 29’ *5 (T. timopheevii Zhuk./T. tauschii (Coss.) Schmal.),
carrying Rg2; line (1A ‘CS’ × ‘Strela’) with Rg3. The red glume colour in 21 cultivars of Triticum aestivum and in the accession
of T. petropavlovskyi has been shown to be determined by the single gene Rg1, located on chromosome 1B. Five cultivars carrying
the gene Rg3 for red glumes on chromosome 1A have been revealed. The cultivars ‘Zhnitsa’ and ‘Iskra’ carry the gene Rg3 alone.
The red glume colour in the cultivars ‘Milturum 321’, ‘Milturum 2078’, ‘Sredneural'skaya’ is controlled by two genes, Rg1
and Rg3. In two common wheat cultivars, ‘Sarrubra’ and ‘Krasnoyarskaya 1103’ the red glume colour is determined by Rg1, inherited
from local populations (‘Turka’ and ‘Kubanka’ respectively) of tetraploid wheat T. durum Desf. var. hordeiforme Host. Wide
occurrence of the Rg1 gene in common wheat has been confirmed. On the contrary, none of the investigated varieties carries
the gene Rg2.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
12.
Hoy-Taek Kim Yutaka Hirata Yong-Uk Shin Hae-Sung Hwang Jeong-Hwan Hwang Il-Sheob Shin Dae-Il Kim Sang-Jo Kang Hyun-Jung Kim Dong-Young Shin Ill-Sup Nou 《Euphytica》2004,138(1):73-80
The pear cultivar ‘Osa-Nijisseiki’ (S2Ssm
4; sm = stylar-part mutant) has been used as a parent to breed self-compatible cultivars that produce excellent fruits. However,
determination of the self-compatibility of ‘Osa-Nijisseiki’ offspring requires a lot of time, 6 years or more, by conventional
cross breeding. We have designed a rapid reliable method for the identification of self-compatible varieties of ‘Osa-Nijisseiki’
offspring based on the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) with S-allele specific
restriction endonucleases. By using this method, 8 self-compatible varieties were selected among 16 selections resulting from
a cross between the self-compatible cultivar ‘Osa-Nijisseiki’ (S2Ssm
4) and the self-incompatible cultivars ‘Niitaka’ (S3S9), ‘Whasan’ (S3S5), ‘Chuwhangbae’ (S4S6). The S-genotypes of 16 ‘Osa-Nijisseiki’ offsprings were also determined.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
13.
Salvador Becerra-Rodríguez Víctor Manuel Medina-Urrutia Marciano Manuel Robles-González Timothy Williams 《Euphytica》2008,164(1):27-36
Grapefruit growers in the tropics require information about existing and new citrus cultivars with high productivity potential.
The objective of this study was to determine the growth, yield, and fruit quality performance of seven pigmented and four
white grapefruit cultivars under the dry tropic conditions of Colima, Mexico. The trees were budded on sour orange (Citrus aurantium L.) rootstock and planted at a distance of 8 × 4 m. ‘Oroblanco’ and ‘Marsh Gardner’ white-fleshed grapefruit cultivars and
‘Chandler’, a pink-fleshed pummelo, were the largest trees with the greatest height (5.0–5.6 m), canopy diameter (6.2–6.3 m),
trunk diameter (21.9–23.3 cm), and canopy volume (109–123 m3). Lower height (4.3–4.8 m) and canopy volume (73–96 m3), but with similar canopy diameter to the previously mentioned cultivars, were recorded for the remaining pigmented cultivars.
‘Chandler’ pummelo and four pigmented grapefruit cultivars (‘Shambar’, ‘Río Red’, ‘Ray Ruby’, and ‘Redblush #3’) had yearly
productions of 34.8, 34.9, 34.1, 32.7, and 30.6 ton ha−1, respectively. The most productive white grapefruit cultivar was ‘Marsh Gardner’ (30.5 ton ha−1). Grapefruit cultivars having the largest fruit size showed a higher inverse relationship between fruit weight and yield
than those with small fruit. Most genotypes had higher values of fruit weight, juice content, and maturity index than those
required by the local market. The most promising grapefruit cultivars based on their acceptable growth, yield superior to
30 ton ha−1, and acceptable fruit color were ‘Río Red’, ‘Shambar’, ‘Ray Ruby’, and ‘Redblush #3’. 相似文献
14.
Rong-Cai Yang Mohan R. Thiagarajah Vipan K. Bansal Gary R. Stringam M. Habibur Rahman 《Euphytica》2006,148(3):217-225
Summary The segregation and linkage between glufosinate (transgenes ‘Rf3’ and ‘T177’) and blackleg resistance genes in canola (Brassica napus L.) were assessed using F1 microspore-derived doubled haploid (DH) populations from four crosses including reciprocals, two involving the transgene ‘Rf3’ and the other two involving the transgene ‘T177’. To relax the assumption of no segregation distortion required for the conventional analysis of segregation and linkage, we employed Bailey's analysis that allows detecting segregation distortion at linked loci. The significant departures from the 1:1 segregation were detected in the crosses involving the transgene ‘T177’ but not in the crosses involving the transgene ‘Rf3’. The apparent deficit of the herbicide tolerant DH lines in the crosses with the transgene ‘T177’ is likely due to differential selection against the gametes carrying ‘T177’ during microspore culture. The linkage was strong between blackleg resistance and the transgene ‘Rf3’ but weak or absent between blackleg resistance and the transgene ‘T177’, suggesting that the two transgenes are probably inserted into distant regions of the genome. The observed linkage offers an opportunity to develop new canola cultivars with both glufosinate tolerance conferred by transgene ‘Rf3’ and blackleg resistance. 相似文献
15.
Lei Huang Ailan Zeng Pengyin Chen Chengjun Wu Dechun Wang Zixiang Wen 《Plant Breeding》2018,137(5):714-720
Salinity is a common abiotic stress causing soybean [Glycine max (L.) Merr.] yield loss worldwide. The use of tolerant cultivars is an effective and economic approach to coping with this stress. Towards this, research is needed to identify salt‐tolerant germplasm and better understand the genetic and molecular basis of salt tolerance in soybean. The objectives of this study were to identify salt‐tolerant genotypes, to search for single‐nucleotide polymorphisms (SNPs) and QTLs associated with salt tolerance. A total of 192 diverse soybean lines and cultivars were screened for salt tolerance in the glasshouse based on visual leaf scorch scores after 15–18 days of 120 mM NaCl stress. These genotypes were further genotyped using the SoySNP50K iSelect BeadChip. Genomewide association mapping showed that 62 SNP markers representing six genomic regions on chromosomes (Chr.) 2, 3, 5, 6, 8 and 18, respectively, were significantly associated with salt tolerance (p < 0.001). A total of 52 SNP markers on Chr. 3 are mapped at or near the major salt tolerance QTL previously identified in S‐100 (Lee et al., 2014). Three SNPs on Chr. 18 map near the salt tolerance QTL previously identified in Nannong1138‐2 (Chen, Cui, Fu, Gai, & Yu, 2008). The other significant SNPs represent four putative minor QTLs for salt tolerance, newly identified in this study. The results above lay the foundation for fine mapping, cloning and molecular breeding for soybean salt tolerance. 相似文献
16.
There is a need to identify genetic markers that can assist coconut (Cocos nucifera L.) breeding programs. With that objective,
electrophoretic patterns of leaf peroxidases, endopeptidases and coomassie blue stained proteins were analysed in four cultivars
(‘West African Tall’, ‘Rennell Tall’, ‘Malayan Yellow Dwarf’, ‘Cameroon Red Dwarf’), and in the hybrids PB121 (‘Malayan Yellow
Dwarf’ × ‘West African Tall’) and PB111 (‘Cameroon Red Dwarf’ × ‘West African Tall’). The polymorphisms detected fit the expression
of two alleles of a dimeric peroxidase, two alleles of a monomeric endopeptidase, and a pair of active and null alleles of
a coomassie blue stained protein. Four distinctive genotypes were identified. One for each of the tall cultivars, another
for both of the dwarf cultivars, and the last for both of the hybrids. Applications of the markers in breeding programs, and
in research concerning reproductive biology and phylogeny, are discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
17.
Celso Lopes de Albuquerque Frederico Denardi Adriana Cibele de Mesquita Dantas Rubens Onofre Nodari 《Euphytica》2011,181(2):277-284
Apple plants are self-incompatible because a genetic mechanism allows the female reproductive organ to recognize and reject
self-pollen or pollen from genetic related individuals and allows non-self pollen to effect fertilization. Thus, there are
implications to both breeding strategies and orchard management for fruit production. The purpose of this study was to identify
and to characterize the S-RNase alleles of the gametophytic incompatibility among apple cultivars developed in Brazil, seeking
to give support for choosing right combinations of parent in the apple breeding programs. It also sought to identify correct
combinations of scion/pollinator cultivars of commercial apple orchards. A total of 16 specific S-RNase alleles primers were
tested against DNA extracted from 12 Brazilian cultivars and their parents. The molecular analysis confronted to the reference
cultivars, showed that the cultivars Daiane, Imperatriz and Princesa have the same incompatibility S3 and S5 alleles, while
‘Lisgala’ showed the alleles S2 and S5; ‘Suprema’, S1 and S9; ‘Catarina’, S1 and S19; ‘Joaquina’ and ‘Fred Hough’, S5 and
S19; ‘Baronesa’, S3 and S9; ‘Duquesa’, S2 and S3. For ‘Primícia’ and ‘Condessa’ it was only possible to identify one of the
S-alleles, namely S24 and S2, respectively, with the second allele remaining to be identified. Progeny test indicated the
Mendelian inheritance for RNase alleles. Results of this study will be helpful to judiciously choose parents in apple breeding
programs to improve compatibility. 相似文献
18.
The development of triploid cultivars from crosses between the tetraploid landrace ‘Morado de Huetor’ and diploid commercial
cultivars was studied in this work. Fertility of both 4x × 2x and 2x × 4x crosses, as measured by fruit set and number of
seeds per fruit, and germination rate of triploid seeds, were studied in order to assess the viability of producing commercial
seeds. Seven triploid hybrid progenies were included in a progeny test together with thirteen experimental tetraploid hybrids
and four cultivars (‘Grande’, ‘UC157-F2’, ‘Purple Passion’ and ‘Morado de Huetor’). Marketable spear production and mean diameter
of spears were evaluated in both 2007 and 2008. Crosses 4x × 2x were more successful than 2x × 4x, and a wide variation from
high to very low fertility within tetraploid females was found. Germination rate of triploid seeds was similar to diploid
ones. After 2 years of evaluation experimental hybrid triploids were more productive than tetraploids with values similar
to the highest yielding diploid cultivar (‘Grande’). Spear diameter of triploid hybrids was in general thicker than the tetraploid
hybrids and significantly thinner than the standard cultivars ‘Grande’ and ‘Purple Passion’ (tertraploid). These results suggest
that the development of new triploid cultivars (4x × 2x) employing a tetraploid landrace from a different genetic background
of current diplods could be interesting in asparagus breeding, broadening the spectrum of cultivated asparagus cultivars. 相似文献
19.
Genetic and molecular characterization of resistance to Heterodera glycines race isolates 1, 3, and 5 in Peking 总被引：2，自引：0，他引：2
Soybean cyst nematode (SCN), Heterodera glycines Ichinohe, has caused severe damage to soybean [Glycine max (L.) Merr.] worldwide
since its discovery in 1954. ‘Peking’ is one of the most important sources in breeding SCN resistant soybean cultivars because
it is resistant to Races 1, 3, and 5. Genetic information on SCN Races 1, 3, and 5 from Peking is essential to efficiently
develop resistant soybean cultivars. Resistance to Race 3 in Peking was found to be controlled by three genes, but little
is known on the inheritance of resistance to Races 1 and 5, and whether alleles conditioning resistance to Races 1 and 5 belong
to the same linkage group and are allelic to genes giving resistance to Race 3. To determine the genetic bases of resistance
to SCN Races 1, 3, and 5, Peking was crossed to the susceptible line ‘Essex’ to generate F1 hybrids. The F2 population and F 2:3 families were advanced from the F1 and evaluated for resistance to SCN Race isolates 1, 3, and 5. Resistance to H. glycines Race isolates 1, 3, and 5 in Peking
was found to be conditioned by three genes, one dominant and two recessive (Rhg, rhg, rhg). Peking may share similar sets
of resistance loci between Races 1 and 3, but not between Races 3 and 5, or between Races 1 and 5.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
20.
Genetic characterization and identification of new accessions from Syria in an olive germplasm bank by means of RAPD markers 总被引：4，自引：0，他引：4
Angjelina Belaj Juan M. Caballero Diego Barranco Luis Rallo Isabel Trujillo 《Euphytica》2003,134(3):261-268
Thirty-two olive cultivar accessions from Syria, most of them obtained from collecting expeditions, were characterized by
means of RAPD markers before being introduced in the World Germplasm Bank of Cordoba. A total of 79 polymorphic bands(6.1
polymorphisms per primer) out of 93(7.1 bands per primer) were scored for the13 primers used, corresponding to 84.9% of the
amplification products. Thirty-one different genotypes were clearly discriminated. Differences were not found among the amplification
profiles from different individuals of the same cultivar. Only two cases of mislabeling or errors of planting were found.
Fourteen accessions corresponding to 6 homonyms were discriminated by RAPDs as different genotypes. The dendrogram obtained
by RAPD analysis included three major groups. Some evidence of relationships of the Syrian accessions studied according to
their geographic origin and/or diffusion was found. For instance, cultivars from the Central Syria (Tadmur/Palmyra)such as
Toffahi', ‘Abbadi Abo Gabra’-1033,‘Abo Kanani’, ‘Shami’-1041, ‘Abbadi Shalal’ ‘Adgam’-844 and ‘Majhol’-1013 clustered in Group
1 and 2. Six cultivars from Northern Syria clustered in Group 2. But it was not found a geographic structure for the cultivars
from South and West of Syria. These results agree with the hypothesis of autochthonous origin of most of the olive cultivars.
Some associations between cultivars from Central Syria and their fruit size were observed. This suggests that fruit size was
a criterion of local selection in olive cultivars of this area.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献