首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 15 毫秒
1.
Micrometam C is a core of novel marine compound isolated from the mangrove associates Micromelum falcatum. In this study, we investigated the protective effects of micrometam C in inflammation models in the transgenic zebrafish line Tg (corola: eGFP) and RAW264.7 macrophages. We found that micrometam C significantly suppressed the migration of immune cells in tail-cutting-induced inflammation in transgenic zebrafish and reduced lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) in both zebrafish and macrophages. In addition, micrometam C also restored LPS-induced reduction of endogenous antioxidants, such as catalase (CAT), glutathione (GSH) and superoxide dismutase (SOD). The protective effects of micrometam C were in parallel to its inhibition of NADPH oxidase and nuclear factor-kappa-binding (NF-κB) activity. Thus, the present results demonstrate that micrometam C protects against LPS-induced inflammation possibly through its antioxidant property.  相似文献   

2.
研究沉香叶黄酮稳定性及对H2O2诱导HepG2细胞氧化损伤的保护作用。通过测定溶液吸光值,探讨温度、光照、金属离子、pH值对沉香叶黄酮稳定性的影响。建立H2O2诱导HepG2细胞氧化损伤模型,将细胞分为正常组、模型组以及沉香叶黄酮低、中、高剂量组,采用水溶性四唑盐法检测细胞增殖率,2′,7′-二氢二氯荧光黄双乙酸钠法（DCFH-DA）测定细胞内活性氧（ROS）水平,生化试剂盒检测细胞乳酸脱氢酶（LDH）释放量、丙二醛（MDA）含量、细胞内总超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）活性。结果表明,沉香叶黄酮在温度70 ℃以下,pH 3~7范围内,Na+、K+、Mg2+、Ca2+金属离子中,黑暗环境与节能灯光中较稳定;能显著抑制H2O2造成的细胞死亡,降低胞内ROS与MDA的生成,减轻LDH向细胞外扩散程度,提高SOD、CAT、GSH-Px活性。沉香叶黄酮在低温、弱酸、部分金属离子、弱光条件下具有一定稳定性;对H2O2诱导HepG2氧化应激损伤具有保护作用,其作用可能与调节细胞氧化还原系统、清除胞内活性氧水平、提高细胞内抗氧化酶系活性有关。  相似文献   

3.
为了探讨细胞质对越冬期杂种小麦生理特性的影响,以四种同核异质雄性不育系(K、B、Ven、S型不育系)及其恢复系R113的杂种F_1为材料,测定了其在田间自然条件下越冬期叶片与抗寒性相关的生理生化指标。结果表明,在K型、B型、Ven型、S型杂交组合F_1子代中,S型杂交F_1叶片中SOD、POD、CAT活性、可溶性糖、游离脯氨酸和叶绿素含量最高,而H_2O_2、O_2~-·、MDA含量、电导率低于其他雄性不育杂交F_1。由此可见,在配制杂种时,S型杂种F_1的抗寒性较好,通过选择S型不育系可以改善杂种F_1的越冬期抗冻能力。  相似文献   

4.
In this work, we designed and synthesized a series of amide derivatives (1–13), benzoxazine derivatives (16–28) and amino derivatives (29–30) from xyloketal B. All 28 new derivatives and seven known compounds (14, 15, 31–35) were evaluated for their protection against H2O2-induced HUVEC injury. 23 and 24 exhibited more potential protective activities than other derivatives; and the EC50 values of them and the leading compound 31 (xyloketal B) were 5.10, 3.59 and 15.97 μM, respectively. Meanwhile, a comparative molecular similarity indices analysis (CoMSIA) was constructed to explain the structural activity relationship of these xyloketal derivatives. This 3D QSAR model from CoMSIA suggested that the derived model exhibited good predictive ability in the external test-set validation. Derivative 24 fit well with the COMSIA map, therefore it possessed the highest activity of all compounds. Compounds 23, 24 and 31 (xyloketal B) were further to examine in the JC-1 mitochondrial membrane potential (MMP) assay of HUVECs using flow cytometry (FCM). The result indicated that 23 and 24 significantly inhibited H2O2-induced decrease of the cell mitochondrial membrane potential (ΔΨm) at 25 μM. Collectively, the protective effects of xyloketals on H2O2-induced endothelial cells may be generated from oxidation action by restraining ROS and reducing the MMP.  相似文献   

5.
For making full use of aquatic by-products to produce high value-added products, Siberian sturgeon (Acipenser baerii) cartilages were degreased, mineralized, and separately hydrolyzed by five kinds of proteases. The collagen hydrolysate (SCH) generated by Alcalase showed the strongest 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·) and hydroxide radical (HO·) scavenging activity. Subsequently, thirteen antioxidant peptides (SCP1-SCP3) were isolated from SCH, and they were identified as GPTGED, GEPGEQ, GPEGPAG, VPPQD, GLEDHA, GDRGAEG, PRGFRGPV, GEYGFE, GFIGFNG, PSVSLT, IELFPGLP, LRGEAGL, and RGEPGL with molecular weights of 574.55, 615.60, 583.60, 554.60, 640.64, 660.64, 885.04, 700.70, 710.79, 602.67, 942.12, 714.82, and 627.70 Da, respectively. GEYGFE, PSVSLT, and IELFPGLP showed the highest scavenging activity on DPPH· (EC50: 1.27, 1.05, and 1.38 mg/mL, respectively) and HO· (EC50: 1.16, 0.97, and 1.63 mg/mL, respectively), inhibiting capability of lipid peroxidation, and protective functions on H2O2-damaged plasmid DNA. More importantly, GEYGFE, PSVSLT, and IELFPGLP displayed significant cytoprotection on HUVECs against H2O2 injury by regulating the endogenous antioxidant enzymes of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) to decrease the contents of reactive oxygen species (ROS) and malondialdehyde (MDA). Therefore, the research provided better technical assistance for a higher-value utilization of Siberian sturgeon cartilages and the thirteen isolated peptides—especially GEYGFE, PSVSLT, and IELFPGLP—which may serve as antioxidant additives for generating health-prone products to treat chronic diseases caused by oxidative stress.  相似文献   

6.
在非生物胁迫条件下,海藻糖可以提高植物的抗逆性,减少逆境胁迫对植物组织的伤害,维持植物的相对正常生长。为了解其具体的作用机制,以小麦为材料,通过检测高温胁迫及室温恢复过程中丙二醛(MDA)含量、过氧化氢(H2O2)含量、超氧自由基(O·-2)含量、抗氧化物质[抗坏血酸(AsA)、还原型谷胱甘肽(GSH)]含量、抗氧化酶[超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)]活性及其基因转录的表达调控,以探究外源海藻糖对小麦幼苗抗氧化系统的影响。结果表明,在高温胁迫下外源海藻糖处理主要提高了AsA含量,增强了CAT和APX活性,同时上调了Mn-SOD、Cu/ZnSOD、CAT、POD和APX的相对表达量,从而降低了MDA及H2O2的产生。而随后的室温恢复过程基本与高温胁迫的结果一致,也提高了这些酶的基因转录水平以及AsA含量,主要差别是对抗氧化酶活性的影响,即室温恢复阶段外源海藻糖主要提高了POD和APX活性。综合来看,外源海藻糖在小麦幼苗的高温胁迫及室温恢复过程中,通过促进抗氧化酶基因的上调表达,提高抗氧化酶活性和抗氧化物质含量,以酶促和非酶促两种机制共同清除高温胁迫产生的活性氧,减少氧化胁迫的损伤,维持小麦幼苗的生长。  相似文献   

7.
为探讨茶黄素（Theaflavin,TF）对过氧化氢（H2O2）诱导的血管内皮细胞氧化应激损伤的保护效应及作用机制,将人脐静脉血管内皮细胞HUVEC分为对照组、损伤组（0.2 mmol·L-1 H2O2处理）和TF预处理组（2.0、5.0、10.0 μmol·L-1 TF和0.2 mmol·L-1 H2O2处理）。损伤组和TF组均以H2O2处理24 h,其中TF组先以不同浓度TF预处理2 h,对照组均以定量溶剂处理。以MTT法测定细胞活力,DCFH-DA探针测定活性氧（ROS）水平,流式细胞术检测细胞凋亡,Western blot测定蛋白表达水平,相应试剂盒测定乳酸脱氢酶（LDH）、一氧化氮（NO）、丙二醛（MDA）含量及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化氢酶（GSH-Px）活性。结果显示,与对照组相比,损伤组细胞活力显著降低,LDH释放量增加,NO水平降低,胞内ROS水平升高,MDA增加,抗氧化酶活力降低,细胞凋亡升高;TF预处理能够显著提高细胞活力,降低LDH水平,维持NO水平,降低胞内ROS水平及MDA的产生,提高抗氧化酶活力,并抑制细胞凋亡;进一步研究显示,TF能够激活Nrf2/HO-1通路,且Nrf2抑制剂会显著降低TF的内皮细胞保护效应。可见,TF能够有效抑制H2O2诱导的血管内皮细胞氧化应激损伤,其机制至少部分是通过激活Nrf2/HO-1通路实现的。  相似文献   

8.
本文研究了白背天葵多酚的超临界CO2提取工艺及其体内外抗氧化活性,为白背天葵的开发利用提供依据。以萃取压力、萃取时间、萃取温度、CO2流量和夹带剂浓度为单因素,以多酚提取得率为指标,采用响应面法对提取工艺条件进行优化。采用体外抗氧化活性试验分析提取物对DPPH及ABTS自由基的清除能力。体内抗氧化活性试验以D-半乳糖致衰小鼠为模型,白背天葵多酚提取物150、300、600 mg/kg连续灌胃30 d,测定其血清及肝组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）活性和丙二醛（MDA）含量。结果表明,白背天葵多酚的超临界CO2最优提取工艺为萃取压力35 MPa、萃取时间2 h、萃取温度40 ℃、CO2流量20 L/h,多酚提取得率为5.32%。体外抗氧化试验表明,多酚对DPPH及ABTS自由基的清除率随其浓度的提高而提高。与空白组比较,衰老模型组血清及肝组织中的SOD、GSH-Px、CAT活性显著性降低,MDA含量显著性升高,白背天葵多酚提取物能显著提高衰老模型组血清及肝组织中的SOD、GSH-Px、CAT活性,并降低MDA含量。  相似文献   

9.
Background: Cryopreservation of pre-antral follicles is a hopeful technique to preserve female fertility. The aim of the present study was to evaluate reactive oxygen species (ROS) and total antioxidant capacity (TAC) levels of mouse vitrified pre-antral follicles in the presence of alpha lipoic acid (ALA). Methods: Isolated pre-antral follicles (140–150 µm in diameter) were divided into vitrified–warmed and fresh groups. Each group was subjected to in vitro maturation with or without ALA for 12 days, followed by adding human chronic gonadotropin to induce ovulation. In vitro fertilization was performed to evaluate their developmental competence. In parallel, the amount of ROS and TAC were assessed after 0, 24, 48, 72, and 96 h of culture by 2'',7''-dichlorofluorescin assay and ferric reducing/antioxidant power assay, respectively. Results: The respective rates of survival, antrum formation, and metaphase II oocytes were significantly higher in ALA-supplemented groups compared to the groups not treated with ALA. TAC and ROS levels were significantly decreased and increased, respectively during the culture period up to 96 h in the absence of ALA in both vitrified and non-vitrified samples. However, with pretreatment of ALA, TAC levels were increased significantly and remained constant up to 96 h in vitrified-warmed pre-antral follicles, while ROS levels completely returned to the level of starting point after 96 h of culture in the presence of ALA. Conclusion: Pretreatment of ALA positively influences development of pre-antral follicles in vitrified and non-vitrified samples through increasing follicular TAC level and decreasing ROS levels. Key Words: Vitrification, Pre-antral follicle, Alpha lipoic acid (ALA), Reactive oxygen species (ROS), Total antioxidant capacity (TAC)  相似文献   

10.
测定长尾潜蝇茧蜂（Diachasmimorpha longicaudata）寄生的4日龄橘小实蝇（B. dorsalis）幼虫体内过氧化氢酶（CAT）、过氧化物酶（POD）、超氧化物歧化酶（SOD）、酚氧化物酶（PO）活性。结果表明：幼虫在被寄生后,在其免疫系统的调节下产生大量的活性氧自由基（ROS）,致使被寄生的幼虫较未被寄生的3种抗氧化物酶（CAT、POD、SOD）活性明显高,然而被寄生的幼虫PO活性明显低于未被寄生的幼虫。这4种酶协同作用,从而有效减少寄主体内过量的ROS,保证寄生蜂能有效地寄生,提  相似文献   

11.
The effects of aging from t = 0–108 h at two different temperatures (4 and 25 °C) on the non-linear viscoelastic rheological properties and secondary protein structure of hard wheat flour dough (HWD) were investigated using large amplitude oscillatory shear tests (LAOS) coupled with Fourier transform infrared spectroscopy (FTIR) and SDS-PAGE. Storage (G') and loss (G'') moduli rapidly decreased during aging at 25 °C. Subjecting HWD to progressively longer aging times at 25 °C caused dramatic changes in the non-linear viscoelastic properties demonstrated by strain softening (negative values of e3/e1) and shear thinning (negative values of v3/v1) behavior. Elastic Lissajous curves of the unaged control dough showed clockwise turn and wider elliptical trajectories as dough aging proceeds especially at higher temperatures. Other non-linear LAOS parameters (G'M-G''L, η'M-η'L, S and T) supported that aging process at higher temperature caused a progressive change in dough structure from strain stiffening to strain softening behavior while dough samples aged at 4 °C showed fairly close behavior with the control dough sample. FTIR spectra indicated that the relative content of β-sheet and β-turn structures decreased while the content of α-helix structure increased for all dough samples as a result of dough aging. SDS-PAGE results supported the breakdown of high molecular weight (HMW) and low molecular weight (LMW) glutenin subfractions. Aging at the higher temperature of 25 °C decreased the HMW/LMW ratio from 0.77 to 0.59, while the ratio was 0.73 for the dough aged at 4 °C which is fairly close to the control sample. Our results show that the degradation rate of gluten/starch network was triggered by aging at higher temperature, longer aging time, and natural fermentation which resulted in increasing acidity and increase in endogenous proteolytic and amylolytic activity, and also increasing gluten solubility and break down of intermolecular disulfide bonds at acid pH.  相似文献   

12.
为了解黄顶菊对小麦的化感效应,研究了黄顶菊茎叶、根水浸提液对小麦根系活力、叶片细胞膜透性和抗氧化特性的影响。结果表明,随提取液质量分数的提高,小麦根系活力、SOD、POD及CAT活性逐渐降低,而叶片细胞膜透性、MDA的含量逐渐上升。从化感效应指数RI和化感综合效应指数SE比较来看,黄顶菊茎叶对小麦的化感抑制效应大于根浸提液。  相似文献   

13.
The inheritance of trichome density and droplet size was investigated in diploid and tetraploid crosses. In haploidTuberosum ×Solanum berthaultii and (S.phureja ×haploid Tuberosum) × S.berthaultii crosses, droplet size on type B trichomes was small or droplets were lacking on F1 hybrids. Parental phenotypes were recovered in the F2 and backcross generations. Trichome density was more variable within a clone that was droplet size. Density of both type A and type B trichomes appears to be under the control of small numbers of genes, as parental phenotypes were recovered in the F2 and backcross generations. The variation observed for each of the three traits studied could not be explained by hypotheses based on the segregation of one or two loci. The insect resistance ofSolanum berthaultii appears to be due to the interaction of several chemical and physical factors, thus potato breeders should handle it as a quantitatively inherited trait.  相似文献   

14.
This study was designed to examine the protective effects of the marine brown algae Petalonia binghamiae against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms. P. binghamiae methanol extract (PBME) prevented hydrogen peroxide (H2O2)-induced growth inhibition and exhibited scavenging activity against intracellular reactive oxygen species (ROS) induced by H2O2 in mouse-derived C2C12 myoblasts. PBME also significantly attenuated H2O2-induced comet tail formation in a comet assay, histone γH2A.X phosphorylation, and annexin V-positive cells, suggesting that PBME prevented H2O2-induced cellular DNA damage and apoptotic cell death. Furthermore, PBME increased the levels of heme oxygenase-1 (HO-1), a potent antioxidant enzyme, associated with the induction of nuclear factor-erythroid 2 related factor 2 (Nrf2). However, zinc protoporphyrin IX, a HO-1 competitive inhibitor, significantly abolished the protective effects of PBME on H2O2-induced ROS generation, growth inhibition, and apoptosis. Collectively, these results demonstrate that PBME augments the antioxidant defense capacity through activation of the Nrf2/HO-1 pathway.  相似文献   

15.
This study was carried out to investigate the protective effects of chitosan nanoparticles (CNP) against hydrogen peroxide (H2O2)-induced oxidative damage in murine macrophages RAW264.7 cells. After 24 h pre-incubation with CNP (25–200 μg/mL) and chitosan (CS) (50–200 μg/mL, as controls), the viability loss in RAW264.7 cells induced by H2O2 (500 μM) for 12 h was markedly restored in a concentration-dependent manner as measured by MTT assay (P < 0.05) and decreased in cellular LDH release (P < 0.05). Moreover, CNP also exerted preventive effects on suppressing the production of lipid peroxidation such as malondialdehyde (MDA) (P < 0.05), restoring activities of endogenous antioxidant including superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) (P < 0.05), along with increasing total antioxidant capacity (T-AOC) (P < 0.05). In addition, pre-incubation of CNP with RAW264.7 cells for 24 h resulted in the increase of the gene expression level of endogenous antioxidant enzymes, such as MnSOD and GSH-Px (P < 0.05). At the same concentration, CNP significantly decreased LDH release and MDA (P < 0.05) as well as increased MnSOD, GSH-Px, and T-AOC activities (P < 0.05) as compared to CS. Taken together, our findings suggest that CNP can more effectively protect RAW264.7 cells against oxidative stress by H2O2 as compared to CS, which might be used as a potential natural compound-based antioxidant in the functional food and pharmaceutical industries.  相似文献   

16.
小麦新品种烟农5158及其亲本旗叶生理特性研究   总被引：1，自引：0，他引：1  
在盆栽条件下,以小麦新品种烟农5158及其亲本鲁麦14号、烟农15号和烟航选2号为材料,研究烟农5158旗叶生理特性与亲本之间的差异.结果表明,烟农5158及其亲本旗叶净光合速率均在开花期达到最大,之后逐渐下降,在生育后期差异显著,烟农5158旗叶净光合速率始终高于其亲本.与亲本相比,烟农5158旗叶中抗氧化酶SOD、POD和CAT活性在生育后期下降缓慢且维持较高水平;烟农5158及其亲本旗叶中的SOD、POD和APX活性均随生育进程推进呈先升高后降低的趋势;花后7～28 d,烟农5158旗叶中POD活性显著高于其亲本.烟农5158与其亲本相比具有较高的可溶性蛋白含量,生育后期具有较低的MDA含量.烟农5158在生育后期具有叶片衰老缓慢、净光合速率高、抗逆性强的特点,这可能与它开花后旗叶中抗氧化酶活性较高、可溶性蛋白含量较高和MDA含量较低有关.  相似文献   

17.
SeveralSolatium tuberosum clones were tested for resistance to the reniform nematode,Rotylenchulus reniformis. Commercial cultivars La Rouge and possibly Red La Soda had high levels of resistance to the reniform nematode.R. reniformis resistance was found to exist independently of the H1, H2, or H3 genes for resistance to races of potato cyst nematodes,Globodera rostochiensis andG. pallida. In addition to reniform nematode resistance, B7642-2, B7154-8 and B7680-6 contained the H1 gene and breeding linePI 377741 contains the H1 and H2 gene for potato cyst nematode resistance. Over a three month period reniform nematode counts were significantly lower in pots where resistant varieties were grown.  相似文献   

18.
为了提高小麦的抗旱能力,通过转基因将拟南芥 RD29A:DREB1A转入到小麦品种陇春30中,成功获得三个单拷贝本底DREB1A蛋白低表达水平的纯合转基因家系,并在干旱胁迫下测定了其生理指标及产量相关农艺性状。结果表明,与陇春30相比,转基因小麦的脯氨酸和可溶性糖含量以及SOD、CAT和POD活性都显著提高,H2O2含量、MDA含量和相对导电率均显著降低,株高、穗长、穗粒数、千粒重、地上生物量显著增加,说明 RD29A:DREB1A外源基因的导入能够显著增强陇春30的抗旱性。  相似文献   

19.
The relationship between active oxygen metabolism and resistance to late blight (Phytophthora infestans) in potato (Solanum tuberosum L.) was studied for 72 h post-inoculation by comparing three resistant cultivars (low disease index) with three susceptible ones (high disease index). Activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), and the content of ascorbic acid (ASA), were higher in the resistant cultivars than in the susceptible ones. The production rate of the superoxide anion radical (O2?) was lower in the resistant cultivars than in the susceptible ones. These changes, which were associated with the potato plant’s response to infection with P. infestans, provide some insight into the physiological basis of resistance and may also provide a screening tool for resistance to late blight.  相似文献   

20.
胚乳裂纹对玉米种子萌发过程中保护酶活性的影响   总被引：2，自引：1，他引：1  
以玉米杂交种种子为试验材料,研究胚乳无裂纹、胚乳单裂纹、胚乳双裂纹和胚乳龟裂文种子在萌发过程中超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）3种保护酶活性以及丙二醛（MDA）含量和细胞膜透性的变化规律。结果表明,随着种子萌发进程推进,其SOD、POD、CAT活性和细胞膜透性均呈逐步上升趋势,保护酶活性升高幅度表现为POD最大、CAT次之、SOD最小;不同胚乳裂纹类型种子在萌发过程中,其保护酶活性均表现为龟裂文种子 <双裂纹种子 <单裂纹种子 <无裂纹种子。种子萌发过程中其MDA含量呈逐步下降的变化趋势,并表现为龟裂文种子中MDA含量最高,双裂纹的种子次之,无裂纹的种子最低。说明胚乳裂纹对种子萌发代谢具有不利影响,在种子生产中应该减少胚乳裂纹产生。  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号