共查询到20条相似文献，搜索用时 234 毫秒
1.
2.
用PCR和PCR-RFLP方法检测和鉴定进口海鱼中的异尖线虫幼虫 总被引：1，自引：0，他引：1
应用PCR和PCR-RFLP方法对厦门口岸进口的各种海鱼中的异尖线虫幼虫进行检测鉴定。结果在带鱼、竹荚鱼、金线鱼、大眼鲷和白姑鱼等海鱼中发现了简单异尖线虫、典型异尖线虫、对盲囊线虫和针回线虫等4种异尖线虫。带鱼和竹荚鱼的异尖线虫感染率达100%,竹荚鱼异尖线虫感染强度最高,白姑鱼感染率和感染强度相对较低。少数海鱼同时感染2种以上异尖线虫,从带鱼中同时检出简单异尖线虫和针蛔线虫,从竹荚鱼中检出简单异尖线虫、对盲囊线虫和典型异尖线虫。内切酶实验结果表明,应用限制性内切酶HinfI有效鉴别上述4种异尖线虫。 相似文献
3.
应用PeR和PCR—RFLP方法对厦门口岸进口的各种海鱼中的异尖线虫幼虫进行检测鉴定。结果在带鱼、竹荚鱼、金线鱼、大眼鲷和白姑鱼等海鱼中发现了简单异尖线虫、典型异尖线虫、对盲囊线虫和针回线虫等4种异尖线虫。带鱼和竹荚鱼的异尖线虫感染率达100％,竹荚鱼异尖线虫感染强度最高,白姑鱼感染率和感染强度相对较低。少数海鱼同时感染2种以上异尖线虫,从带鱼中同时检出简单异尖线虫和针蛔线虫,从竹荚鱼中检出简单异尖线虫、对盲囊线虫和典型异尖线虫。内切酶实验结果表明,应用限制性内切酶UinfI有效鉴别上述4种异尖线虫。 相似文献
4.
采用“害获灭”针剂、“７０５１灭虫丁”、“灭虫丁”粉剂、单甲脒和敌百虫等５种药物进行驱杀猪体内外寄生虫试验，对猪疥螨均有杀灭作用，前３种对体内蛔虫、毛首线虫、食道口线虫、肺线虫的杀虫率达１００％、敌百虫的驱虫率为８６％，对肺线虫不起作用，单甲脒对体内寄生虫克驱虫作用，敌百虫因毒性大，有残留，有待淘汰。 相似文献
5.
6.
柳富荣 《养殖与饲料．饲料世界》2004,(3):21-22
月鳢[Channa asiatica(Linnaeus)]，俗称七星鱼、点星鱼、点秤鱼等，属鲈形目、鳢科、月鳢属，是一种优质小型淡水野生鱼类。月鳢肌肉中粗蛋白质含量为21．8％～22．5％，粗脂肪2.3％～2.7％，肉嫩少刺，营养丰富，味道鲜美，可食率达76％，且具有滋补生肌、活血去湿等多种药用功效，因而深受广大 相似文献
7.
8.
9.
张成文 《养殖与饲料．饲料世界》2008,(3):25-26
鳢鱼肉质坚实细嫩、肌间刺少、营养丰富，具有去淤生新、生肌补血、滋补调养、加速病人手术伤口愈合之功效，我国南方和港澳地区常作为体弱病人、产妇和儿童的高级滋补食品，是经济价值较高的名贵淡水鱼类，素有“鱼中珍品”之美称。我国鳢鱼主要养殖品种有两种：乌鳢[Ophicephalus argus（Cantor）]和斑鳢[Ophicephalus maculatus（Lace-pede）]。 相似文献
10.
11.
Caligid copepods (Crustacea) known as sea lice are pests of cultured fish, causing serious diseases and economic losses in fish aquaculture worldwide. One species, Caligus sclerotinosus Roubal, Armitage & Rohde, 1983 (Caligidae), is considered a serious pest of the highly prized red seabream Pagrus major (Temminck and Schlegel, 1843) (Sparidae) cultured in Japan. Recently, in neighboring Korea, red seabream culture has intensified and almost replaced yellow tail culture. However, until now, there have been no reports on infection of this sea louse from red seabream in Korea. We surveyed 120 (20 fish per month) P. major from a sea ranched Tongyeong Marine Research Center aquaculture facility, Gyeongsangnamdo, Korea for six months in 2011 (June to November). We recorded severe infection by the sea louse C. sclerotinosus on the skin of P. major. Prevalence was 100%, mean intensity 7.06, maximum intensity 49, and minimum intensity 2. Adult females (624), males (219) and few chalimi (5) were observed and identified by their morphology. As an average of all our collections, less than 0.6% of individuals were chalimi. We suggest, therefore, that adults of C. sclerotinosus undergo ontogenetic host switching after their final moult. No infection of C. sclerotinosus was found on wild P. major collected from Tongyeong and Yeosu fish markets on the southern coast of Korea. Severe infection by this sea louse may cause secondary infections of the host. This copepod is already reported from Australia and Japan and hence, this is the first report from Korea. We expect this pest to have an impact on Korean red seabream fisheries equally serious to that being experienced in Japan. 相似文献
12.
JiíMLKOVSK 《中国鸟类》2011,2(2)
山麻雀是一种广布的小型鸣禽,拉丁学名通常为Passer rutilans (Temminck).经作者考证,该种名发表于1836年12月31日,但在此之前,Gould已于1836年4月8日发表了该种并命名为Passer cinnamomeus.因此,根据动物命名法中的优先原则,该种应称作Passer cinnamomeus (Gould,1836).其公认的3个亚种也应分别称作P.cinamomeus rutilans (Temminck,1836),P.cinnamomeus intensior Rothschild,1922及P.cinnamomeus cinnamomeus (Gould,1836). 相似文献
13.
苜蓿草粉对黄河鲤鱼消化生理指标的影响 总被引：1，自引：0，他引：1
选取来源一致、体质健康、尾均重为248.70±1.31 g的黄河鲤鱼750尾,采用单因子完全随机设计,设5个处理,3个重复,分别在基础饲粮中添加0%(对照组)、5%(试验Ⅰ组)、10%(试验Ⅱ组)、15%(试验Ⅲ组)和20%(试验Ⅳ组)紫花苜蓿草粉,研究其对黄河鲤鱼消化生理指标的影响。结果表明:1)苜蓿草粉的添加对黄河鲤鱼消化道指数无显著影响(P>0.05)。2)肝胰脏和前、中、后各肠段的蛋白酶和淀粉酶活性均随着苜蓿草粉的添加比例的增加呈现先升后降的趋势。其中,5%和10%的苜蓿草粉添加组在肝胰脏和后肠中的蛋白酶活性显著高于对照组(P<0.05);5%和10%添加组在肝胰脏和各个肠段的淀粉酶活性均显著高于对照组(P<0.05);3)适宜水平的苜蓿草粉(5%、10%)能够提高黄河鲤鱼肠绒毛高度,在前肠,10%添加组的肠绒毛高度显著高于对照组(P<0.05);而高含量的苜蓿草粉(15%、20%)则降低了肠绒毛高度。综合表明,在黄河鲤鱼饲粮中添加10%苜蓿草粉能显著提高其消化酶活性及肠绒毛高度。 相似文献
14.
胭脂鱼弹状病毒包涵体在培养细胞中的形成 总被引：7，自引：0，他引：7
从患致死性出血综合征的胭脂鱼（Myxocyprinus asiaticus Bleeker)分离到一种能产生包涵体的弹状病毒，称为胭脂鱼弹状病毒（Chinese sucker rhabdovirus,CSRV)。选择草鱼卵巢细胞系（Grass carp ovaries,CO)为宿主细胞，在接种CSRV3、6、9、12、18h后，分别取样、固定、制片、染色，用光学显微镜和电子显微镜观察。光镜观察结果显示，CSRV包涵体为直径1－10μm的球形胞质包涵体；在宿主细胞中有多个包涵体，宿主细胞的形态和直径都发生了变化，然后裂解和死亡。透射电镜观察结果显示，在包涵体内存在大量病毒颗粒。 相似文献
15.
Ederli NB Bonadiman SF de Moraes Neto AH DaMatta RA de Paula Santos C 《Veterinary parasitology》2008,151(2-4):227-232
The genus Libyostrongylus includes three species, L. douglassii, L. dentatus and L. magnus that occur as parasites in the proventriculus of Struthio camelus. We confirmed a mixed infection by L. douglassii and L. dentatus in farmed ostriches from the southeast of Brazil for the first time, and provided new information on some morphological characters that differentiate these species. Adult nematodes collected from the proventriculus of ostriches were observed by light and scanning electron microscopy. Morphologic characterization and morphometric analysis of the nematodes enabled the distinction of both species and corroborated results of prior studies. Specimens of L. dentatus have a buccal capsule with a prominent esophageal tooth. Furthermore, males and females of L. dentatus were larger (4954 and 9347 microm) than those of L. douglassii (3411 and 4229 microm), but measurements for most characters in both species were smaller then those previously reported. Besides, the cephalic structure based on scanning electron microscopy differs, and L. dentatus has thick lips with round papillae, whereas L. douglassii has fine lips with lengthened papillae. The confirmation of both species in South America strongly suggests that the mixed infection may be common in farmed ostriches. 相似文献
16.
Waruiru R.M. Nansen P. Kyvsgaard N.C. Thamsborg S.M. Munyua W.K. Gathuma J.M. Bøgh H.O. 《Veterinary research communications》1998,22(5):325-334
The gastrointestinal tracts of 672 crossbred cattle were obtained from various abattoirs in Kiambu District, Kenya from August 1992 to July 1993, and examined for the presence of gastrointestinal nematodes. Eight nematode species were found in 583 (86.8%) of the animals. The nematodes were, in order of prevalence: Haemonchus placei (67.0%), Cooperia pectinata (53.0%), Cooperia punctata (41.7%), Oesophagostomum radiatum (38.4%), Trichostrongylus axei (24.3%), Nematodirus helvetianus (19.6%), Trichuris globulosa (9.7%) and Strongyloides papillosus (3.6%). The intensity of the nematode infection was moderate; the mean burden being less than 7000 worms. H. placei accounted, on average, for 52.3% of the total burden. The total burden was least during the dry seasons and increased gradually during the rainy seasons. Adult H. placei persisted in the host throughout the year and there was no indication of hypobiosis. The heaviest gastrointestinal worm burdens were detected in 1.5- to 3-year-old animals. These findings are discussed with regard to their relevance for strategic control of gastrointestinal nematodes in cattle. 相似文献
17.
The present study was performed to investigate both the identity and the source of the bacteria responsible for a fatal septicaemia observed in a group of three subadult emerald monitors (Varanus prasinus Schlegel 1839). The emerald monitors were necropsied and examined by light microscopy, including immunohistology, and by electron microscopy. Tissue samples were additionally submitted for bacteriological, virological and parasitological examinations. The virological and parasitological results were noncontributory, whereas the bacteriological investigation resulted in the isolation of gram-positive cocci which were characterized biochemically and serologically and by molecular analysis. The death of the emerald monitors was caused by a partially leukocyte-associated septicaemic infection with streptococci of serological group B of serotype V. Phenotypically and genotypically identical group B streptococci were isolated from the intestine of subadult mice, obtained from the feed used for the monitors. The genotypical characterization included an identical DNA fingerprint of strains of both origins, indicating the epidemiological relation between the feeding mice and the infections of the monitors. 相似文献
18.
甘肃省昆虫病原线虫区系研究 总被引：1，自引：0，他引：1
为查明甘肃省昆虫病原线虫的种类及区系分布,通过大蜡螟诱捕法对甘肃省昆虫病原线虫资源进行了调查,分离得到昆虫病原线虫种群51个,分属于8个种：斯氏线虫属（Steinernema）5种,异小杆线虫属（Heterorhabditis）3种。其中斯氏属3个种A,B,C和异小杆1个种D属于国内新记录种。调查表明：甘肃省昆虫病原线虫的优势种为Steinernema feltiae,昆虫病原线虫的分布与土壤类型及植被类型关系密切,沙壤土和壤土中线虫检出率分别为12.2%和8.8%,较粘土5.3%的检出率高,且在果园、菜地和大田中的线虫检出率分别为10.4%,9.2%和5.2%,较未耕地3.3%的检出率高,说明沙壤土和壤土适于昆虫病原线虫的种群建立,且在有农事活动的地块中昆虫病原线虫分布较多。 相似文献
19.
为明确内生真菌对醉马草根际土壤线虫群落特征的影响,于2014年6,8和10月,对带内生真菌和不带内生真菌醉马草根际土壤进行采样,利用淘洗-过筛-蔗糖离心法分离线虫,根据线虫形态学特征进行鉴定,结果表明,本研究共捕获土壤线虫11889条,分别隶属于线虫动物门2纲6目22科37属,带内生真菌醉马草根际土壤线虫个体密度高于不带内生真菌醉马草,而类群数、多样性指数(H')、均匀度指数(J)和丰富度指数(SR)均低于不带内生真菌醉马草,但处理间差异不显著,表明尽管内生真菌使醉马草根际土壤线虫个体密度有所增加,使类群数有所降低,但是内生真菌的存在对醉马草土壤线虫多样性没有影响;与不带内生真菌醉马草相比,带内生真菌醉马草根际土壤线虫MI指数显著升高,而PPI指数和PPI/MI值显著降低,表明内生真菌在一定程度上确实改变了土壤线虫功能类群组成,其中主要影响植物寄生线虫。 相似文献
20.
Parasitic nematode infection indices were recorded in different fish species collected at Cartagena Bay, North of Colombia. Among 19 studied species, the Mugil genus presented the highest prevalence (83.9-100%), although Sciades herzbergii, Caranx hippos and Centropomus undecimalis were also found infected with nematodes. Parasites were found in the liver, intestinal mesenteries and encysted near the intervertebral joints, with an average parasite abundance of 4.0 ± 0.3 nematodes per fish. Morphological analysis allowed the identification of these nematodes as Contracaecum sp. A small, but positive correlation was found between parasite abundance and length (R=0.294, P<0.001) and weight (R=0.244, P<0.001). In contrast, the correlation between parasite abundance and condition factor was negative (R=-0.191, P<0.001). These results are the first describing the presence of nematodes in several fish species of this ecosystem, and it highlights the need for monitoring parasitism in Mugil species in order to avoid parasite ingestion during fish consumption. 相似文献