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1.
黄藤继代增殖培养和成苗的繁殖体选择   总被引:2,自引:1,他引:1       下载免费PDF全文
试验结果表明,黄藤组培生长健壮,形态正常的不同高度繁殖体,在继代增殖培养和成苗培养过程中,其间存在显著差异,母芽和子芽增殖培养过程中,亦存在着显著差异。高度为1.1-2.0cm的子芽为继代增殖培养的最适繁殖体;高度在2.1-4.0cm的未展叶芽体为伸长培养的理想繁殖体,4cm以上的繁殖体最适于生根诱导。  相似文献   

2.
棕榈藤组培技术研究*   总被引:12,自引:2,他引:12       下载免费PDF全文
经对单叶省藤、黄藤和白藤组织培养系列试验证明:半成熟种胚是棕榈藤组织培养最佳外植体,在RM5-1和RM8-2培养基中,相继各培养50d,即形成完整的试管苗;外植体在总盐浓度为6899mg/L的培养基中,置于690 1x光照度下每天照射16h诱导愈伤组织效果最好;RM6是丛芽诱导和继代培养最理想培养基。一个外殖体经一年的培养增殖,其理论繁殖系数为2.8×10^5/a。组培诱导分化的丛生苗切片显微观测  相似文献   

3.
探讨了大量元素含量对我国主要栽培藤种黄藤、短叶省藤和单叶省藤组培增殖的影响,确定了各大量元素的适宜范围.以优化的配方进行多次继代培养后,抽取离体芽和培养基进行大量元素含量测定,评价配方的好坏和确定多次继代使用是否需要变换配方  相似文献   

4.
碳素营养对毛白杨葡萄试管增殖培养苗的影响   总被引:2,自引:0,他引:2  
对毛白杨和葡萄试管苗增殖培养阶段碳素营养试验的研究表明,在以芽生芽为增殖方式的毛白杨继代培养中,培养基中必须加入适量的外源糖分作为碳素营养使其完成形态建成过程,其后补充CO_2可促进丛生芽苗的生长,并(或)提高芽苗的整齐度;在以单芽为增殖方式的葡萄继代培养中,无糖培养法是可行的,且比常规培养法有明显的优越性。  相似文献   

5.
对毛白杨和葡萄试管苗增殖培养阶段碳素营养试验的研究表明,在以芽生芽的增殖方式的毛白杨继代培养中,培养基中必须加入适量的外源糖分作为碳素营养使其完成形态建成过程,其后补充CO2可促进丛生芽苗的生长,并(或)提高芽苗的整齐度;在以单芽为增殖方式的葡萄继代培养中,无糖培养法是可行的,且比常规培养法有明显的优越必性。  相似文献   

6.
对金叶连翘采用植物离体培养进行快速繁殖,并在离体培养过程中对试管苗的分化、生根、移栽及其相关因素进行了研究。结果表明,继代分化培养基以MS BA4mg/L NAA0.1mg/L、生根培养基1/2MS IBA0.3mg/L为最好,试管苗移栽以珍珠岩为基质,在温室内加盖拱棚成活率达86.5%。经室内炼苗培养30天,移栽大田定植成活率达95%以上。  相似文献   

7.
两种省藤组培成苗芽的选择   总被引:2,自引:1,他引:1       下载免费PDF全文
对单叶省藤、短叶省藤组培快繁成苗芽的选择研究试验结果表明:生长健壮、形态正常的不同高度未展叶单芽组,在成苗培养过程中,高生长、生根率、生根条数和生根长度存在差异。高度在2.1~4.0cm的未展叶单芽为伸长培养和生根诱导培养的最佳高度组,生根诱导培养3个月后,芽苗生长可达长到组培苗移植规格(苗高、根长〉4.0cm)。研究试验结果还显示,2种省藤组培成苗培养过程,伸长及生根诱导培养可浓缩为一步,直接从增殖培养材料中选择2.1~4.0cm的单芽进入生根诱导培养,一步成苗。  相似文献   

8.
几种天南星科荫生观叶植物组织培养研究   总被引:6,自引:0,他引:6  
以天南星科荫生观叶植物绿巨人,绿帝王,红帝王,金帝王为对象,通过不同细胞分裂素浓度及其与生长素不同比例搭配对比植体第一次分化影响,椰汁提取物,赤霉素对外植体芽第一次分化影响及继代过程中细胞分裂素对继代芽增殖的影响;试管苗生根条件,不同种类抗菌素抑菌效果的研究,总结出所选用的四个植物品种外植体分化及继代,生根所需的最适诱导及培养条件,繁殖系数可达3^12~4.5^12,为工厂化快速育苗提供了依据与手  相似文献   

9.
取牡丹石榴春季嫩芽作外植体,进行离体快速繁殖,筛选出最佳增殖、生根培养基,确定了试管苗移栽的最佳基质。培养基MS+BA0.7mg/L IBA0.15mg/L增殖生长的最佳培养基,增殖系数为5.7;培养基1/2MS+NAA0.1mg/L对牡丹石榴试管苗生根培养较适合,生根率为91.4%;在塑料小拱棚中利用蛭石为移栽基质,移栽成活率可达86 .7%。  相似文献   

10.
对黄藤和单叶省藤种质离体培养保存进行了研究.在温度15.5℃、照度300 lx、光照时间12 h*d-1的保存条件下,采用适宜的保存培养基,以丛芽形式,黄藤和单叶省藤种质可安全保存15个月以上不转接,丛芽高生长小于1.0 cm.回接到增殖培养基和增殖条件下,单叶省藤种质能恢复原有的增殖能力,保证了保存培养和增殖培养的可循环性.种质保存后伸长能力和生根能力没有下降,可确保离体种质转换为田间种质.因而,棕榈藤种质离体保存具有一定的可行性,值得进一步研究.  相似文献   

11.

In Scandinavia, moose (Alces alces L.) sometimes cause severe browsing damage to economically-important pine. Moose-vehicle accidents have spurred construction of fences along roads, and these may interfere with moose migration between summer and winter ranges, or the road alone may be a barrier. If this happens and moose build up along roads, landowners may suffer economically. Therefore, this study investigated whether roads, fences or other factors influence the use of young pine stands by moose. Eighty stands along roads in northern Sweden were evaluated in which individually-browsed branches were counted on 9972 pines. Moose browsing was not significantly related to birch (Betula pendula Roth, B. pubescens Ehrh.) density, nor did it differ between pines (Pinus contorta Douglas or P. sylvestris L.). However, increased pine density, site productivity and proximity to a highway were associated with increased browsing. Further large-scale studies are needed to understand moose habitat selection and the effects of roads.  相似文献   

12.

The root systems of 2-yr-old Picea glauca, Picea mariana and Pinus banksiana seedlings were submitted to various frost temperatures during an artificial frost to induce different levels of root damage. Frost-damaged and control seedlings were placed in a greenhouse under high and low soil moisture regimes. Seedling growth and physiology were evaluated periodically. Seedling survival was reduced when root damage reached levels of 60-80%. Root systems of all three species showed partial to total recovery by the end of the experiment. In general, root freezing damage caused reductions in seedling growth, with these reductions becoming less significant over time. Root damage had little to no effect on black spruce and jack pine seedling physiology, while white spruce CO 2 uptake decreased with increasing root damage. Shoot nitrogen content of all three species decreased slightly with increasing root damage.  相似文献   

13.

This study investigated the stand structure in pine, spruce and deciduous forests in the border district of Finland and Russia. A total of 46 mature forest stands was selected as pairs, the members of each pair being as similar as possible with respect to their forest site type, age, moisture and topography. The stands were then compared between the two countries by means of basal areas and number of stems. The proportions of dominating tree species were 2-12% lower, and correspondingly the proportions of secondary tree species higher, in Russian forests. The density of the forest stock was also higher in each forest type in Russia. The forests in the two countries differed most radically in terms of the abundance of dead trees. The amount was two to four times higher in Russian deciduous and spruce forests, and in pine forests the difference was 10-fold. The stand structures indicated that Russian coniferous stands, in particular, were more heterogeneous than intensively managed pine and spruce stands in Finland.  相似文献   

14.

The root collar diameter and the height:diameter ratio are of particular importance in container-grown seedlings where a high density in the containers may produce spindly seedlings. Temperature regimes and light quality are known to affect plant growth. The aim of this study was to identify responses in Picea abies (L.) Karst. seedlings grown with light providing different red:far-red ratios and under temperature regimes with alternating day (DT) and night temperature (NT) from negative (DT < NT) to positive (DT > NT) difference (DIF) between DT and NT. Experiments were conducted in controlled environment chambers and in a daylight phytotron. Only limited thermoperiodic responses appeared in P . abies seedlings with respect to seedling height and dry weight accumulation. The formation of terminal buds, however, was clearly delayed in seedlings grown at negative DIF. The results indicate a requirement for day extension light that is high in far-red, to prevent terminal bud formation under natural short-day conditions. An extended study should be conducted to clarify the minimum level of light intensity and the optimal light quality needed to prevent terminal bud formation under natural short-day conditions.  相似文献   

15.
本文分析了CAD在设计中引起的正面和负面影响,并进行了系统的阐述,从而使设计者在应用中能够保持客观的态度。  相似文献   

16.

The effects of soil scarification (mounding), slash removal and clear-cut age on the natural regeneration in clear-cuts was evaluated using data from four sites in southern Sweden. The treatments were carried out during a good seed and establishment year for birch ( Betula pubescens Ehrh. and B. pendula Roth). Scarification had the strongest positive effect on the density of naturally regenerated seedlings, especially in birch, but also in pine ( Pinus sylvestris L.) and spruce [ Picea abies (L.) Karst]. Slash removal had a positive effect on birch density. No statistically significant effect of clear-cut age was found. In addition, no statistically significant interactions between clear-cut age and scarification or slash removal were found. The ingrowth of field vegetation was the fastest in areas that were not scarified, less rapid in areas scarified on old clear-cuts, and the slowest after scarification in fresh clear-cuts. In conclusion, it may be possible to control the density of birch during a good establishment year for birch. If birch is desired, the best combination of treatments is to remove the slash and scarify; otherwise, these treatments should be avoided.  相似文献   

17.
18.

Based on an enquiry, risk perception among non-industrial private forest owners is described in relation to climate change and forestry hazards. Of the respondents, 11% took action to remedy the effects of climate change. Out of a given set, hazards were ranked according to each respondent's experience of recent substantial financial loss to the estate and in relation to his or her willingness to make investments aimed at risk reduction. For each hazard, the respondent assessed the risk in four classes ranging from very high to negligible risk. Six hazards were considered most problematic in all three aspects: browsing damage, falling timber prices, damage by wind, spruce bark beetle, root rot and pine weevil. A majority of the respondents claimed to take action to reduce the risk associated with at least one hazard, while 35% did not know whether they did. Excluding climate change, the need for decision support was the largest in relation to damage by wind owing to a combination of perceived high risk and a high level of ignorance in relation to whether risk-reducing measures were taken.  相似文献   

19.
In situ produced plant residues contain a mixture of different plant components of varying quality. To assess synergistic or antagonistic interactions occurring during the decomposition and mineralization of such mixtures, individual plant parts (stems, leaves, leaf litter and roots) or the mixture of stems, leaves and leaf litter of the agroforestry species pigeonpea (Cajanus cajan) or of crop residues of peanut (Arachis hypogaea) or of the weed hairy indigo (Indigofera hirsuta) were incubated in pots for 19 weeks. Periodically, remaining plant residues were sieved out (>2 mm), weighed and N content as well as soil mineral N determined. Above- and below-ground residues of peanut decomposed fastest and showed the largest N release in agreement with their high N concentration and low-acid detergent fibre (ADF) : N ratio. Hairy indigo was hypothesized to be of lower quality than pigeonpea because of its high-polyphenol content. However, it decomposed faster than pigeonpea, largely because of the higher N and lower lignin concentration of its components. Ranking of individual plant components for N mineralization resulted in the following pattern, leaves > leaf litter > roots > stems. In mixtures of the different plant components a similar species order in decomposition was obtained, e.g. peanut > hairy indigo > pigeonpea. The amount of N released from the mixture was dominated by stem material that comprised 46–61% of the mixture. The interactions in mixtures were relatively small for peanut (generally high-quality components) as well as for pigeonpea (low proportion of high-quality components, i.e. N rich leaf material). However, a positive interaction occurred during later stages of N mineralization in the mixture of hairy indigo as it had a significant proportion of N rich components and absence of highly reactive polyphenols. Thus, for plants with low to intermediate chemical quality attributes, manipulating plant composition (e.g. by varying harvest age, affecting stem and leaf proportions) will be important to obtain significant interactions during decomposition when its components are mixed.  相似文献   

20.
The use of organic waste materials such as milk sewage as an organic fertilizer could have the dual advantages of organic-waste disposal and reduced dependence on inorganic fertilizers. The effects of fertilization with (1) conventional mineral fertilization, (2) milk sewage sludge at 40 kg N ha−1 target rate and (3) no fertilization on pasture production and tree growth were examined in an experiment consisting of two pasture mixtures under a one-year-old Pinus radiata plantation with a density of 2500 trees ha−1. The two pasture mixtures were: (1) Dactylis glomerata L. var. saborto (25 kg ha−1) + Trifolium repens L. group Ladino (4 kg ha−1) + Trifolium pratense L. var. Marino (1 kg ha−1); (2) Lolium perenne L. var. Tove (25 kg ha−1) + Trifolium repens L. group Ladino (4 kg ha−1) + Trifolium pratense L. var. Marino (1 kg ha−1). The experiment began in the spring of 1995 using a randomized block design with three replicates in Castro Riberas de Lea (Lugo, Galicia, north-western Spain). Plot size was 12 × 8 m2, with a 1 m buffer strip between plots. Two-year data showed that fertilization with either material had a positive effect on pasture production, with no significant difference between the two fertilization treatments. Tree growth in the milk sewage sludge plot was significantly higher than in the control plots. Inorganic fertilization increased pasture production, but affected tree growth negatively. The results show that milk sewage sludge could be used as a fertilizer in silvo-pastoral systems. This revised version was published online in June 2006 with corrections to the Cover Date.  相似文献   

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