共查询到19条相似文献，搜索用时 247 毫秒
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地衣芽孢杆菌CHB6高芽孢形成率发酵条件的研究 总被引：1，自引：0，他引：1
以CHB6的细胞生物量和芽孢率为指标,采用正交试验方法,研究了无机盐离子对地衣芽孢杆菌CHB6的细胞生长量及芽孢形成的影响,通过优化获得地衣芽孢杆菌CHB6的细胞生长量和芽孢形成率均较高的培养基配方。结果表明,Mn2+离子对CHB6的影响最显著,最佳培养基配比为:糖蜜1.5%、大豆蛋白胨0.5%、MnSO4.H2O 0.04%、MgSO4.7H2O 0.05%、CaCl2 0.1%、K2HPO4.3H2O 0.1%、KH2PO4 0.125%、NaCl0.5%。细胞生长量可达4.8×109 CFU/mL,芽孢形成率达到90.6%。 相似文献
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内生解淀粉芽孢杆菌TB2液体发酵条件的研究 总被引：3，自引：0，他引：3
采用单因素试验和正交试验对具有广谱拮抗作用的内生解淀粉芽孢杆菌TB2菌株的摇瓶发酵培养基和发酵条件进行优化。结果表明,最佳培养基组份为:玉米淀粉1.0%、豆粉1.0%、(NH4)2SO40.5%、MgSO4·7H2O0.1%、FeSO4·7H2O0.03%、K2HPO4·3H2O0.02%、KH2PO40.01%;培养条件为:初始pH值为7.5,温度35℃,接种量3%,装液量50mL/250mL三角瓶,摇床转速200r/min,发酵周期22～26h。优化条件下发酵水平活菌数达到8.52×1010cfu/mL。 相似文献
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响应面法优化绿色木霉H06产孢发酵培养基 总被引：2，自引：0，他引：2
采用响应面法对绿色木霉H06菌株产孢发酵培养基进行了优化。首先利用Plackett-Burman实验设计筛选出影响产孢的3个主要因素:玉米粉、大豆粕和KH2PO4。在此基础上运用最陡爬坡路径法逼近最大响应值区域,最后利用响应面分析法确定主要因子之间的交互作用及最佳条件。结果表明,蔗糖10 g/L、玉米粉12.75 g/L、NH4NO32 g/L、大豆粕4.65 g/L、MgSO4·7H2O 1 g/L、KH2PO43.32 g/L、H06最大理论孢子含量为3.29×109个/mL。经3次平行实验验证,实际平均孢子含量与预测孢子含量相近,比之前的孢子含量提高了197%。 相似文献
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玉米内生细菌YY1菌株高产抗菌物质的发酵条件优化 总被引：1，自引：0，他引：1
玉米内生细菌YY1菌株为枯草芽孢杆菌,对玉米大斑病菌(Setosphaeria turcica)有强烈的拮抗作用,通过产生抗菌物质发挥抑菌活性。以培养基的无菌滤液对玉米大斑病菌的拮抗活性为检测指标,确定其产生抗菌物质所需要的最佳碳源、氮源及无机盐,通过正交试验得到该菌株产生抗菌物质的培养基配方,并对其发酵工艺条件进行优化。结果表明,最佳培养基为可溶性淀粉3.0%,蛋白胨2.0%,KH2PO40.02%,MnCl20.01%。发酵工艺参数优化结果为培养基装液量30 mL/250 mL,接种量4%,培养基初始pH值为9.0,28℃条件下、120 r/min摇床振荡培养48 h。经发酵条件优化后,其抗菌活性有显著提高。 相似文献
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防治眼菌蚊Bt.菌株的筛选与培养条件优化 总被引：1，自引：1，他引：0
通过11株Bt.菌株对平菇厉眼蕈蚊3龄幼虫的室内毒力测定试验,得出91019菌株对平菇厉眼蕈蚊3龄幼虫有较高的毒力效果,其发酵原液和250倍稀释液对平菇厉眼蕈蚊3龄幼虫的校正死亡率分别达到93.10%和82.76%。通过正交试验对91019菌株的各培养条件进行优化,得出理论最佳培养基组分及培养条件为:玉米粉1.0%、黄豆饼粉2.5%、酵母粉2.0%、鱼粉2.0%、蛋白胨0.4%,KH2PO4 0.5 g/L,CaCO3 4.0 g/L,MgSO4.7H2O0.5 g/L,ZnSO4.7H2O 0.02 g/L;接种量2.0%、pH7.0、装液量25 mL、培养温度25℃。 相似文献
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为探讨淡紫拟青霉E7菌株固体放大发酵因子间的关系以获得大量孢子用于田间试验,通过正交试验设计优化固体发酵培养基成分和培养条件组合,并测定外加碳源、氮源、无机盐和装料方式对产孢量的影响。结果表明：以玉米粉+甘蔗渣+麸皮+壳聚糖组成的正交2号配方为最佳复合培养基质,分生孢子产量达7.13×109个/g,以发酵液pH+液相发酵终点+温度+初始含水量组成的正交4号配方为优适培养条件,分生孢子产量达8.97×109个/g;该菌株在优化后的培养基配方中添加0.4％蔗糖、0.2％蛋白胨、0.002％硫酸锰,以双层纱布袋装料按优化后的培养条件放大培养,产孢量最高可达到8.22×1010个/g。优化后的E7菌株发酵产孢培养基基质用量少,发酵成本低,适合于固体放大发酵生产淡紫拟青霉孢子。 相似文献
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研究银耳菌株Tr0040液体深层发酵工艺条件。以单因子试验为基础，确定了在液体发酵培养基关键因子葡萄糖、蛋白胨、MgS04．7H20、VBl浓度对芽孢产量影响的最适区，在此基础上通过响应面法对其进行优化得到最佳配方：1L培养基中葡萄糖33．91g：蛋白胨8．29g，MgS04．7H202-812，VB114．43m2，KH，PO,0．5g。 相似文献
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银耳芽孢遗传多样性分析 总被引：2，自引：1，他引：1
利用ISSR和RAPD分子标记分析了不同来源的14株银耳芽孢多样性.4个ISSR引物和2个RAPD引物总扩增条带数为40条带,其中多态性位点数为39,多态性位点百分比97.50%.平均等位基因位点数为1.9750,平均有效等位基因位点数为1.393 1,物种水平上Nei's基因多样度指数为0.2270,Shannon遗传多样性指数为0.3559.UPGMA聚类方法对14个银耳菌株进行聚类分析,聚类结果表明,福建省内栽培的不同银耳菌种芽孢没有差异,福建省内银耳菌种比较单一,遗传背景相当狭隘;但这些菌株的芽孢与野生采集分离的银耳菌株的芽孢存在一定的差异. 相似文献
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红菇菌丝体液体培养营养因子的筛选 总被引：1，自引：0，他引：1
采用液体培养的方法.研究不同碳源、氮源、无机盐和生长激素单个营养因子对两个红菇菌菌丝生长的影响.结果表明:在室温振荡培养条件下,0443菌株菌丝生长最适宜的碳源为葡萄糖,而0005菌株菌丝生长最适宜的碳源为乳糖;两个菌株对无机氮源(NH4)2SO4的利用率均为最高;不同的无机盐和生长激素对两个菌株菌丝生长的促进作用有一定的差异,ZnSO4和VB1对0443菌株菌丝生长的促进效果最好,而MnCl2和VB2对0005菌株菌丝生长的促进效果最好. 相似文献
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以玉米秸秆为基质,筛选对木霉增殖有利的营养物质,对生防制剂的田间应用提供依据。利用Plack-ett-Burman试验设计筛选影响木霉产孢的主要营养物,采用最陡爬坡试验测定3种营养物的最适浓度范围;通过Box-Benhnken试验设计,对3种营养物浓度进行优化;盆栽试验检测木霉在土壤中的存活情况防治玉米茎基腐病。结果表明,筛选对木霉产孢影响较大的3个营养物质(氯化铵、硫酸钾、七水硫酸亚铁),最佳配方为氯化铵0.97 g/L,硫酸钾0.92 g/L,七水硫酸亚铁0.97 g/L,在此配方条件下孢子产量为2.61×10~9个/g。盆栽试验表明,利用木霉结合秸秆还田和肥料施用,可增强其存活能力和降低玉米茎基腐病的发生率和病情指数。 相似文献
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锰与氮钾配施对冬小麦籽粒蛋白质含量及蛋白质产量的影响 总被引：1，自引：1，他引：0
为确定在河南省典型潮土上合理的锰(Mn)与氮(N)、钾(K)配施方案.提高冬小麦蛋白质含量和蛋白质产量,通过正交区组三因素五水平通用旋转组合设计的田间试验,研究了Mn与N、K配施对冬小麦蛋白质含量及蛋白质产量的影响.结果表明,合理的Mn与N、K配比可显著提高小麦籽粒蛋白质含量与蛋白质产量.N、K2O和MnSO4·H2O分别控制在33.57～42.29、259.30～276.64、163.83～164.63 kg·hm-2时,蛋白质含量和蛋白质产量才会较高.其中,氮肥对籽粒中清蛋白、球蛋白、醇溶蛋白和谷蛋白含量影响较大,对醇溶蛋白、谷蛋白的作用均为Mn>K;对球蛋白的作用则是K>Mn.因此,保证N肥的合理供应是获得较高蛋白质含量、蛋白质产量和各蛋白组分的先决条件,在此基础上根据籽粒产量和品质要求调节Mn肥与K肥的施用比例. 相似文献
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Fangfang Yang Lijuan Long Xiumei Sun Hualian Wu Tao Li Wenzhou Xiang 《Marine drugs》2014,12(3):1245-1257
Lipid production is an important indicator for assessing microalgal species for biodiesel production. In this work, the effects of medium composition on lipid production by Scenedesmus sp. were investigated using the response surface methodology. The results of a Plackett–Burman design experiment revealed that NaHCO3, NaH2PO4·2H2O and NaNO3 were three factors significantly influencing lipid production, which were further optimized by a Box–Behnken design. The optimal medium was found to contain 3.07 g L−1 NaHCO3, 15.49 mg L−1 NaH2PO4·2H2O and 803.21 mg L−1 NaNO3. Using the optimal conditions previously determined, the lipid production (304.02 mg·L−1) increased 54.64% more than that using the initial medium, which agreed well with the predicted value 309.50 mg L−1. Additionally, lipid analysis found that palmitic acid (C16:0) and oleic acid (C18:1) dominantly constituted the algal fatty acids (about 60% of the total fatty acids) and a much higher content of neutral lipid accounted for 82.32% of total lipids, which strongly proved that Scenedesmus sp. is a very promising feedstock for biodiesel production. 相似文献
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Summary The influence of freeze dried potato powder derived from tubers (cvs Désirée and Epicure) exposed to light as a culture medium
on the growth, number and spore size of nine fungal pathogens was determined in vitro with particular emphasis on the role
of glycoalkaloids. Greater reductions in pathogen growth reflected increased exposure to light of tubers prior to freeze drying.
High correlations (% R2 adj) were obtained between glycoalkaloid concentration and growth rate of the majority of pathogens tested. Where spores
were produced, numbers were in general significantly less when cultured on freeze dried powder derived from tubers exposed
to light. No effects on spore size ofFusarium species were recorded, but spore length of remaining pathogens was reduced and spore width increased, with the exception
ofC. coccodes where spore length and width was increased following culture onto freeze dried powder derived from tubers exposed to light. 相似文献
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为建立高效的节节麦幼胚再生体系,以节节麦幼胚为外植体,通过正交设计,探索基本培养基、2,4-D、碳源、KT等因素对幼胚愈伤组织诱导、分化及植株再生效果的影响。结果表明,4种因素中,基本培养基对节节麦幼胚愈伤组织诱导的影响最显著(P0.05),2,4-D浓度对节节麦幼胚愈伤组织诱导也有显著影响(P0.05),添加3.0mg·L~(-1) 2,4-D的培养基诱导出的愈伤组织质量高,淡黄色,表面呈不规则颗粒状,质地致密,再生频率可以达到17.62%。KT浓度对节节麦愈伤分化影响最显著,基本培养基、2,4-D和碳源对节节麦幼胚愈伤分化均无显著影响。不同碳源对节节麦幼胚愈伤组织诱导和分化的影响均不显著,为节约成本可直接选用30g·L~(-1)蔗糖作为碳源。节节麦幼胚组织培养的最佳组合是:愈伤诱导培养基为MS培养基+3mg·L~(-1) 2,4-D+15g·L~(-1)蔗糖+15g·L~(-1)甘露醇,愈伤分化培养基为MS培养基+15g·L~(-1)蔗糖+15g·L~(~(-1))甘露醇+1.0mg·L~(-1) KT。 相似文献
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In two field experiments acetylene (C2H2) reduction by white clover in mixed swards was compared to N2-fixed measured by 15N dilution. In both experiments, samples for C2H2 reduction were 7.5 cm diameter turves taken from plots within which microplots of 24 cm diameter were delimited and to which 15N was applied as ammonium sulphate (15NH4)2SO4). C2H2 reduction was assayed every 6–7 d. The rate of C2H2 reduction per unit length of stolon was applied to the estimated stolon length within the appropriate microplot at the time of assay, and the amount of C2H2 that would have been reduced within the microplot was estimated by integration. In experiment 1, turves taken from grass/clover swards to which 0, 1·5, 3·0, 4·5 or 6·0 g N m?2 had been applied were incubated in sealed chambers (10% C2H2, 90% air). The mean ratio of C2H2 reduced to N2 fixed during 5 weeks was 0.74:1. Application of N fertilizer lowered the proportion of assimilated N derived from N2 fixation from 95% in unfertilized swards to 83% in those receiving 6 g N m?2 (60 kg N ha?1). In experiment 2, clover roots and stolons from plots that previously had been grazed were dissected from turves and incubated in a stream of C2H2 and air (i.e. the open system). The maximum rate of ethylene (C2H4) produced during the first 12 min was taken as a measure of true nitrogenase activity. The relationship between C2H2 reduced and N2 fixed was significant (r=0.80**). The mole ratio was 0.55:1 for the 6 weeks duration of the experiment, the low ratio possibly being due to disturbance of the nodules adversely affecting acetylene reduction. Mole ratios from both experiments were well short of the theoretical 4·3:1. Using the open system does not, therefore, overcome the shortcomings of the acetylene reduction technique for measuring N2 fixation of white clover in mixed swards. 相似文献
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B. Yahuaca-Juárez H. E. Martínez-Flores J. A. Huerta-Ruelas R. C. Pless P. A. Vázquez-Landaverde R. Tello Santillán 《Plant foods for human nutrition (Dordrecht, Netherlands)》2013,68(1):65-71
The objective of this work was to evaluate the effect of alkaline cooking on the oxidative stability of oil in corn flour. A central composite design was used to study the combined effect of lime concentration (%) and steep time (h) on peroxide value (PV); specific extinction coefficients at 232 and 270 nm (K232 and K270); and FTIR absorbance at 3009 cm?1, 3444 cm?1, and 3530 cm?1 in oils from corn flour obtained by alkaline cooking. The results indicate that lime concentration and steep time affected the PV, K232, and K270. A decrease of 2.56 % was observed in the IR absorption bands, corresponding to the polyunsaturated fatty acids. The FTIR spectra also showed absorption bands related to the secondary oil oxidation products. 相似文献