共查询到20条相似文献，搜索用时 203 毫秒
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功能基因组学研究进展 总被引：1，自引：0，他引：1
基因组研究计划包括以全基因组测序为目标的结构基因组学和以基因功能鉴定为目标的功能基因组学2方面的内容.作者对功能基因组的主要研究方法:微阵列或DNA芯片、表达序列标签法、基因表达系列分析法、蛋白质组学分析法以及反向遗传学技术等进行了简要介绍,同时综述了上述各种技术的优缺点以及部分生物功能基因组的研究进展. 相似文献
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基因芯片技术是20世纪90年代发展起来的一门高新技术,具有高通量、灵敏度高,特异性好等特点.它是通过微阵列技术,将高密度DNA片段阵列,通过点样或原位合成法的形式,以一定的顺序或者排列方式使其附着在如玻璃片等固相表面,借助碱基互补杂交原理,进行大量病原基因表达系谱分析、检测、基因分型等.本文简要介绍了基因芯片技术在动物疫病诊断中的应用及其发展前景. 相似文献
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分子病理学是将病理学、细胞生物学和细胞生物化学结合起来,在分子水平上研究疾病发生机理的一门病理学分支。20世纪80年代以来,聚合酶链反应、比较基因组杂交、荧光原位杂交、高通量DNA测序和各种生物芯片等一大批生物学技术的相继问世,以及基因组计划的成功进行,为开发组织标本中丰富的分子生物学信息提供了重要的技术支撑,极大地推动了分子病理学的快速发展。目前,国内外常用的一些分子病理学检测技术有PCR技术、直接DNA测序、毛细管阵列杂交、荧光原位杂交、比较基因组杂交、流式细胞术、组织微阵列、原位杂交、DNA和cDNA芯片等。… 相似文献
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动物疫病基因芯片诊断技术的研究进展 总被引：2，自引：0，他引：2
基因芯片(Gene Chip)又称DNA芯片或DNA微阵列(DNAmicroarray),是将大量的DNA片段按预先设计的排列方式固化在载体表面,并以此作为探针,在一定的条件下,与样品中待检测的靶基因片段杂交,通过检测杂交信号,实现对靶基因的存在、含量及变异等信息的快速检测。它是分子生物学和微细加工技术(micro fabrication technology)相结合的产物。自从1996年美国Affymetrix公司成功地制作出世界上首批用于药物筛选和实验室试验用的生物芯片,并制作出芯片系统,此后世界各国在芯片研究方面快速前进,不断有新的突破。它是生物芯片(Biochip)中发展最成… 相似文献
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基因芯片及其在病原微生物检测中的应用 总被引：2，自引：0，他引：2
基因芯片是近年来迅速发展的一门生物高新技术,它以其能够快速、高效、大规模地同步检测生物遗传信息的卓越功能而得到发展。在基因测序、基因表达分析、药物筛选、基因诊断等领域显示出重要的理论和实用价值。作者就基因芯片技术的原理、技术要点以及在病原微生物检测中的应用作一综述。 相似文献
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近年来，随着测序技术的迅速革新和发展，甲基化研究持续升温。DNA甲基化修饰是在不改变DNA序列的前提下，调控细菌基因表达，改变遗传表现的一种方式。真核生物的DNA甲基化修饰在疾病发生、植物生长等领域的应用已有大量研究。但是，对于原核生物DNA甲基化修饰的研究却较少。为了更好地了解原核生物中细菌的DNA甲基化研究现状，本文对近年致病菌和乳酸菌的甲基转移酶、甲基化组学和检测技术的研究进行简要概述，以期为DNA甲基化在乳酸菌中的应用中提供一定参考。 相似文献
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Jung JW Park JS Hwang JW Kang KS Lee YS Song BS Lee GJ Yeo CD Kang JS Lee WS Jeon KS Um CH Kim YS Oh MJ Youn JP Li P Park JE Hwang SY 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1329-1333
The recent DNA microarray technology enables us to understand a large number of gene expression profiling. The technology has potential possibility to comprehend mechanism of multiple genes were related to compounds which have toxicity in biological system. So, the toxicogenomics through this technology may be very powerful for understanding the effect of unknown toxic mechanisms in biological system. We have studied that the effect of compounds related to hepatotoxin in vivo system using DNA microarray and classified chemicals which have been well characterized. We have studied three compounds; 2 peroxisome proliferators: Clofibrate (ethyl-p-chlorophenoxyisobutyrate), gemfibrozil (5-2[2,5-dimethyl-phenoxy]2-2-dimethyl-pentanonic), and an antiepileptic drug: phenytoin (5,5-diphenylhydantoin). Male Sprague-Dawely VAF(+) albino rats of 5-6 weeks old were treated with each compound for 24 hr and 2 weeks. 4.8 K cDNA microarray in house has been used for gene expression profiling. We found that the clustering of gene expression had similarity like as the toxic phenotype of compounds. 相似文献
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公共和私有的EST计划为大量物种的基因表达研究提供了大量利用EST的机会。为了估计EST数据库中基因的表达水平，可以通过计算每一种基因在不同组织、不同发育阶段和不同条件下构建的cDNA文库中的出现次数，来获得基因表达的有关信息。这些信息水平的量与常规的Northern blots不同，可以允许不同基因之间表达水平的比较。而且当EST资料来源于不同发育阶段的cDNA文库时，还可以了解不同发育阶段的基因表达谱。由电子Northern杂交分析所获得的基因表达资料可以通过应用高密度DNA点阵得到证实并扩展于高表达基因领域之外。相关ESTS可以标定于尼龙膜或玻璃上并用相关组织的一链总cDNA作为探针进行鉴定。双色荧光标记可以得到精确的mRNA比率测定。在不远的将来，包含一种机体所有基因互补物的高密度DNA点阵将成为整个基因组范围基因表达类型分析的有效工具。 相似文献
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Motoshige YasuikeHidehiro Kondo Ikuo HironoTakashi Aoki 《Comparative immunology, microbiology and infectious diseases》2011,34(2):103-110
The glycoprotein (G protein) gene, but not the nucleocapsid protein (N protein) gene, of the hirame rhabdovirus (HIRRV) was previously shown to be highly effective in inducing a protective immune response in Japanese flounder (Paralichthys olivaceus) when used as a DNA vaccine. Our previous cDNA microarray analysis demonstrated that interferon-stimulated genes (ISGs) were strongly induced by the HIRRV G protein gene (pHRV-G) but not by the N protein gene (pHRV-N). However, the molecular basis for the difference in protective immunity between pHRV-G- and pHRV-N-vaccinated fish during HIRRV infection is still unclear. In this study, we use a DNA microarray to analyze differences of gene expression in pHRV-G- and pHRV-N-vaccinated fish during HIRRV infection. Microarray analyses showed substantial difference in gene expression patterns during HIRRV infection between fish vaccinated with pHRV-G and pHRV-N. In addition, genes having homology to mammalian T cell activation-related genes were up-regulated in the HIRRV G protein-vaccinated group. 相似文献
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cDNA microarray gene expression analysis and toxicological phenotype for anticancer drug 总被引：1，自引：0，他引：1
Lee GJ Lee WS Jeon KS Um CH Kim YS Kim SJ Lee CH Yoon HK Hwang SY Park JS Hwang JW Kang KS Lee YS Kim MS Chon KJ Yeo CD Kang JS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1339-1345
Toxicogenomics, the subdiscipline that merges genomics with toxicology, hold the promise to contributing toward the goal of elucidating mechanism by studying genomic profiling related with various drugs. The application of gene expression profiling technology to examine multiple genes and signaling pathways promises a significant advance in understanding the toxic mechanisms of various drugs and prediction of new drug candidate. Toxicogenomics is emerging field combining genomics and bioinformatics to identify and characterize mechanisms of toxicity of drug and various compounds. The principal hypothesis underlying on this field is that chemical-specific pattern of altered gene expression is related with each chemicals properties, especially toxicological property, and it will be revealed using high-density microarray analysis of sample from exposed organisms. So, in this study we compare the gene expression pattern of two anticancer drugs paclitaxel and orally absorbable paclitaxel, using the cDNA microarray. And from the result of this study, it is possible to provide the new possibility for genome-wide insight into mechanism of their anticancer activity and toxicological phenotype. 相似文献
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