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1.
The effects of 15N-labelled urea, (NH4)2SO4 and KNO3 on immobilization, mineralization, nitrification and ammonium fixation were examined under aerobic conditions in an acid tropical soil (pH 4.0) and in a neutral temperate soil (pH 6.8). Urea, (NH4)2SO4 and KNO3 slightly increased net mineralization of soil organic nitrogen in both soils. There was also an apparent Added Nitrogen Interaction (ANI) i.e. added labelled NH4-N stood proxy for unlabelled NH4-N that would otherwise have been immobilized. So far as immobilization and nitrification were concerned, urea and (NH4)2SO4 behaved very similarly in each soil. Immobilization of NO3-N was negligible in both soils. Some of the added labelled NH4-N was rapidly fixed, more by the temperate soil than by the tropical soil. This labelled fixed NH4-N decreased during incubation, in contrast to labelled organic N, which did not decline.  相似文献   

2.
The symbioses between Trifolium subterraneum, mycorrhizal fungi and Rhizohium are affected by (NH4)2SO4 and by the nitrification inhibitors 2-chloro-6 (trichloromethyl) pyridine (N-Serve) and 2-trichloromethyl pyridine (2TMP). At 50 μg · g?1 soil N-Serve and 2TMP had toxic effects on plant growth, measured as leaf expansion, root length and dry weight. Lower concentrations of N-Serve also produced some toxic symptoms. The addition of (NH4)2SO4 to the soil at 2 and 6 m-equiv NH+4 per pot, resulted in reduced root length and nodulation. Shoot dry weight was reduced at 6 m-equiv NH+4 per pot. In the presence of (NH4)2SO4 the toxic effects of the nitrification inhibitors on plant growth were less.Both nitrification inhibitors reduced development of mycorrhizal entry-points and extent of root colonization (% infection). Percentage infection of the root system was also reduced by (NH4)2SO4. Development of nodules on the lateral roots was increased in the presence of N-Serve at 5 and 15 μ · g?1. This effect, however, was accompanied by a marked reduction in N2ase activity. Smaller increases in nodulation were apparent with 2TMP and were associated with variable N2ase activity.  相似文献   

3.
For understanding the effects of nitrification ability on nitrogen (N) use efficiency and N losses via denitrification in paddy soils under flooding conditions, six paddy soils with different nitrification activities were sampled from various sites of China and a pot experiment was conducted. Rice plants at tillering stage were transplanted into pots and harvested 7.5 days after transplanting, 15N-(NH4)2SO4 was applied 2.5 days after rice transplanting under continuously flooding conditions. The N losses by denitrification were determined by the unrecovered 15N applied as 15NH4 + and the N use efficiency (NUE) was calculated by 15N taken up by rice plants. Plant height (from 33.8 to 37.3 cm) and biomass (from 1.07 g pot?1 to 1.52 g pot?1) increased significantly with the native NH4 + concentration in the studied soils (P < 0.01). The NUE decreased, whereas the N losses via denitrification increased due to the increase in the nitrification rate of soils determined at 60% water holding capacity (P < 0.05). The results implied that the nitrification activity of paddy soils is a key factor in controlling NUE and N losses via denitrification.  相似文献   

4.
A laboratory-based aerobic incubation was conducted to investigate nitrogen(N) isotopic fractionation related to nitrification in five agricultural soils after application of ammonium sulfate((NH4)2SO4). The soil samples were collected from a subtropical barren land soil derived from granite(RGB),three subtropical upland soils derived from granite(RQU),Quaternary red earth(RGU),Quaternary Xiashu loess(YQU) and a temperate upland soil generated from alluvial deposit(FAU). The five soils varied in nitrification potential,being in the order of FAU YQU RGU RQU RGB. Significant N isotopic fractionation accompanied nitrification of NH+4. δ15N values of NH+4 increased with enhanced nitrification over time in the four upland soils with NH+4 addition,while those of NO-3 decreased consistently to the minimum and thereafter increased. δ15N values of NH+4 showed a significantly negative linear relationship with NH+4-N concentration,but a positive linear relationship with NO-3-N concentration. The apparent isotopic fractionation factor calculated based on the loss of NH+4 was 1.036 for RQU,1.022 for RGU,1.016 for YQU,and 1.020 for FAU,respectively. Zero- and first-order reaction kinetics seemed to have their limitations in describing the nitrification process affected by NH+4 input in the studied soils. In contrast,N kinetic isotope fractionation was closely related to the nitrifying activity,and might serve as an alternative tool for estimating the nitrification capacity of agricultural soils.  相似文献   

5.
Abstract

Urea applications to soil are subject to loss by ammonia (NH3) volatilization, unless incorporated. It has been proposed that this loss can be reduced by stimulating populations of soil nitrifiers by an ammonium sulfate [(NH4)2SO4] pretreatment two to four weeks before urea application. The objective of this laboratory trial was to evaluate this concept with five diverse soils, two North American Mollisols and three South American Oxisols. The soils were incubated untreated for two weeks, followed by pretreatment with 0 or 5 kg nitrogen (N) ha‐1 as (NH4)2SO4, on a soil surface area basis. After another two weeks of incubation, the soils were treated with the equivalent of 0 or 50 kg N ha‐1 as urea. Ammonia loss was estimated after trapping into phosphoric acid (H3PO4). Ammonium sulfate pretreatment reduced NH3 loss with the two Mollisols and a sandy Oxisol and increased the recovery of the urea application as mineral [ammonium (NH4 +) + nitrate (NO3 ‐)] N in these soils. Little NH3 loss was detected from the two clay Oxisols, and (NH4)2SO4pretreatment did not influence NH3 loss or recovery of urea as mineral N. An example of a cropping system where this concept may have utility is discussed.  相似文献   

6.
This study was conducted to examine whether the applications of N-inputs (compost and fertilizer) having different N isotopic compositions (δ15N) produce isotopically different inorganic-N and to investigate the effect of soil moisture regimes on the temporal variations in the δ15N of inorganic-N in soils. To do so, the temporal variations in the concentrations and the δ15N of NH4+ and NO3− in soils treated with two levels (0 and 150 mg N kg−1) of ammonium sulfate (δ15N=−2.3‰) and compost (+13.9‰) during a 10-week incubation were compared by changing soil moisture regime after 6 weeks either from saturated to unsaturated conditions or vice versa. Another incubation study using 15N-labeled ammonium sulfate (3.05 15N atom%) was conducted to estimate the rates of nitrification and denitrification with a numerical model FLUAZ. The δ15N values of NH4+ and NO3− were greatly affected by the availability of substrate for each of the nitrification and denitrification processes and the soil moisture status that affects the relative predominance between the two processes. Under saturated conditions for 6 weeks, the δ15N of NH4+ in soils treated with fertilizer progressively increased from +2.9‰ at 0.5 week to +18.9‰ at 6 weeks due to nitrification. During the same period, NO3− concentrations were consistently low and the corresponding δ15N increased from +16.3 to +39.2‰ through denitrification. Under subsequent water-unsaturated conditions, the NO3− concentrations increased through nitrification, which resulted in the decrease in the δ15N of NO3−. In soils, which were unsaturated for the first 6-weeks incubation, the δ15N of NH4+ increased sharply at 0.5 week due to fast nitrification. On the other hand, the δ15N of NO3− showed the lowest value at 0.5 week due to incomplete nitrification, but after a subsequence increase, they remained stable while nitrification and denitrification were negligible between 1 and 6 weeks. Changing to saturated conditions after the initial 6-weeks incubation, however, increased the δ15N of NO3− progressively with a concurrent decrease in NO3− concentration through denitrification. The differences in δ15N of NO−3 between compost and fertilizer treatments were consistent throughout the incubation period. The δ15N of NO3− increased with the addition of compost (range: +13.0 to +35.4‰), but decreased with the addition of fertilizer (−10.8 to +11.4‰), thus resulting in intermediate values in soils receiving both fertilizer and compost (−3.5 to +20.3‰). Therefore, such differences in δ15N of NO3− observed in this study suggest a possibility that the δ15N of upland-grown plants receiving compost would be higher than those treated with fertilizer because NO3− is the most abundant N for plant uptake in upland soils.  相似文献   

7.
Summary Nitrification activity (formation of NO 2 – + NO 3 – per unit soil weight) was measured in the surface layer of 15 presubmerged soils incubated in petri dishes under flooded but aerobic conditions. soils with pH above 5 nitrified quickly, whereas soils with pH below this level did not nitrify or nitrified slowly. The pH values between 7 and 8.5 were optimal for nitrification. Organic-matter levels in the 15 soils of our study did not influence their nitrification activities. In a follow-up greenhouse pot study, after a period of 3 weeks, 15N-balance measurements showed that the loss of N through apparent denitrification did not follow the nitrification patterns of the soils observed in the petri dishes. Apparent denitrification accounted for 16.8% and 18.9% loss of 15N from a soil with insignificant nitrification activity and a soil with high nitrification activity, respectively. These results, thus, indicate a lack of correspondence between the nitrification activities of soil and the denitrification loss of N when the former was measured in the dark and the latter was estimated in the light. Soils that nitrified in the darkness of the incubator did not nitrify in the daylight in the greenhouse.  相似文献   

8.
The effects on nitrification and acidification in three subtropical soils to which (NH4)2SO4 or urea had been added at rate of 250 mg N kg−1 was studied using laboratory-based incubations. The results indicated that NH4+ input did not stimulate nitrification in a red forest soil, nor was there any soil acidification. Unlike red forest soil, (NH4)2SO4 enhanced nitrification of an upland soil, whilst urea was more effective in stimulating nitrification, and here the soil was slightly acidified. For another upland soil, NH4+ input greatly enhanced nitrification and as a result, this soil was significantly acidified. We conclude that the effects of NH4+ addition on nitrification and acidification in cultivated soils would be quite different from in forest soils. During the incubation, N isotope fractionation was closely related to the nitrifying capacity of the soils.  相似文献   

9.
Nitrification inhibitors (N-Serve, ATC, and CS2) were added to soils without N fertilizers. While the amount of nitrification of NH4+-N was reduced, so was the amount of ammonification of soil N. This effect was greater with ATC and CS2 than with N-Serve. In three field experiments, the application in the fall of ATC at 22 kg ha?1 mixed into the soil reduced the loss of soil mineral N in early spring. Apparently, the inhibition suppressed both ammonification and nitrification of soil N during the winter, and consequently there was less NO?3 in soil when the wet period occurred in the spring.  相似文献   

10.
Soil was incubated under greenhouse conditions with plant residues having varying phenolic and nitrogen contents. The total plant material added in staggered applications every 4 months was 15 g kg?1 soil and the total incubation period was 12 months.The N-mineralization in these plant residues as influenced by their phenol and N contents was examined. The nitrification of applied (NH4)2SO4 in these amended soils was also investigated under optimum conditions of pH.A high plant-N content resulted in increased N-mineralization of plant residue, but this effect was lowered by the presence of high concentrations of polyphenols in the decomposing residue, most probably due to increased participation of N with polyphenols in the formation of humus fractions.Soils amended with phenol-rich residues did not show any inhibition of nitrification of applied (NH4)2SO4. Possible reasons are discussed. In organic matter decomposition, the quality of the leaf polyphenols appears to determine the degree of inhibition to soil nitrification.  相似文献   

11.
土壤水湿状况和肥料碳氮比对稻田肥料氮素转化的影响   总被引：2，自引：0，他引：2  
朱培立  黄东迈 《土壤学报》1986,23(3):251-261
本文应用15N示踪法,测定并探讨了土壤中三种水分状况及四种不同C/N值肥料对肥料氮素转化的影响,试验结果表明:土壤水分和肥料C/N值均对水稻产量有较大的影响,相比之下,土壤水分的影响似更大.水稻对肥料氮的吸收利用率,淹水栽植高于旱植,氮素固定在旱地条件下作用加强,淹水并有一定渗漏的土壤上肥料氮的损失最大,示踪结果说明从土壤渗漏液中淋失的氮素80%以上为土壤固有氮素,相对而言肥料氮的损失较低.试验还表明肥料中碳氮值与肥料氮的吸收利用率之间呈负相关,与肥料残留率呈正相关.此外,本试验还测定了土壤水湿状况和肥料碳氮值在土壤氮素转化中的作用,讨论了当土壤氮素矿化和固定作用相等时,有机肥的碳氮临界值及其实用意义.  相似文献   

12.
蔡祖聪 《土壤学报》2003,40(2):239-245
用15N分别标记尿素和KNO3,研究了淹水条件下 ,黄泥土和红壤性水稻土的无机氮转化过程及尿素和KNO3对氮素转化过程的影响。结果表明 ,淹水条件下 ,土壤中存在15NH 4 的成对硝化和反硝化过程。红壤性水稻土15NH 4 硝化只检测到15NO- 2 ,但有反硝化产物15N2 生成 ,因此 ,很可能存在着好气反硝化过程。15NO- 3浓度的下降符合一级反应方程 ,黄泥土的速率常数几乎是红壤性水稻土的 1 0倍。反硝化过程和DNRA过程共同参与15NO- 3的还原。加入尿素提高土壤pH ,增加黄泥土DNRA过程对反硝化过程的基质竞争能力 ,但反硝化过程仍占绝对优势。加入尿素或KNO3改变土壤pH是导致对无机氮转化影响有所不同的主要原因 ,浓度的作用较为次要。  相似文献   

13.
烤烟钾素库源关系生理调控措施研究   总被引：8，自引：2，他引：8  
在大田生产条件下 ,采用单株挂牌设置处理的研究方法 ,研究涂抹生长素与其它不同生长调节剂和烟株顶端调控等措施对烤烟钾吸收及其在库源中再分配的影响。结果表明 ,现蕾后不打顶或打顶结合生长素处理茎断面均有利于不同部位叶片含钾量的提高 ,以打顶结合涂抹生长素效果更好 ;内源生长素和外源生长素都有利于烟株体内的钾素向叶片中分配 ,其中外源生长素的活性更强 ,能更有效地提高烟株叶片的含钾量 ,且涂抹 2次比涂抹 1次的效果好 ,但涂抹次数间差异不显著。  相似文献   

14.
A loam from the Frilsham and one from the Wickham Series were incubated at 50 and 90 per cent of their water contents at saturation with 100 μg NH4NO3-Ng?1 soil in the presence and absence of C2H2 (0.5 per cent, v/v). Acetylene inhibited nitrification in both soils, but had no effect on mineralization of N. No denitrification (measured as the production of N2O in the presence of C2H2) occurred during incubation at 50 per cent saturation. At 90 per cent saturation, denitrification resulted in a loss of 28.4 and 36.7 μg Ng?1 after 48 h from the Frilsham and Wickham soils, respectively. The concurrent inhibition of nitrification had no effect on the extent of denitrification at this time. In the Wickham soil, NO3? was exhausted after 168 h incubation in the presence of C2H2 and denitrification was underestimated by 13 μg Ng?. The data suggested that concurrent inhibition of nitrification during measurement of denitrification using the C2H2 inhibition technique is most likely to affect the estimate of denitrification loss when NO3?supply is limited by the inhibition of nitrification.  相似文献   

15.
A better understanding of the nitrogen (N) cycle in agricultural soils is crucial for developing sustainable and environmentally friendly N fertilizer management and to propose effective nitrous oxide (N2O) mitigation strategies. This laboratory study quantified gross nitrogen transformation rates in uncultivated and cultivated black soils in Northeast China. It also elucidated the contribution made by nitrification and denitrification to the emissions of N2O. In the laboratory, soil samples adjusted to 60 % water holding capacity (WHC) were spiked with 15NH4NO3 and NH4 15NO3 and incubated at 25 °C for 7 days. The size and 15N enrichment of the mineral N pools and the N2O emission rates were determined between 0 and 7 days. The results showed that the average N2O emission rate was 21.6 ng N2O-N kg?1 h?1 in cultivated soil, significantly higher than in the uncultivated soil (11.6 ng N2O-N kg?1 h?1). Denitrification was found to be responsible for 32.1 % of the N2O emission in uncultivated soil, and the ratio increased significantly to 43.2 % in cultivated soil, due to the decrease in soil pH. Most of the increase in net N2O-N emissions observed in the cultivated soil was resulting from the increased production of N2O through denitrification. Gross nitrification rate was significantly higher in the cultivated soil than in the uncultivated soil, and the ratio of gross nitrification rate/ammonium immobilization rate was 6.87 in cultivated soil, much larger than the uncultivated soil, indicating that nitrification was the dominant NH4 + consuming process in cultivated soil, and this will lead to the increased production of nitrate, whereas the increased contribution of denitrification to N2O emission promoted the larger emission of N2O. This double impact explains why the risk of N loss to the environment is increased by long-term cultivation and fertilization of native prairie sites, and controlling nitrification maybe effective to abate the negative environmental effects.  相似文献   

16.
Incubation studies (5 weeks at 30°C) of nitrification were made in an acid (pH 5.8) and a neutral (pH 7.1) soil receiving varying concentrations of pig slurry and (NH4)2SO4 solution. Mineral-N and pH changes were observed at weekly intervals and inorganic salts media were used to obtain separate estimates of the numbers of NH4-N- and NO2-N-oxidizing bacteria. In the acid soil, pig slurry NH4-N was nitrified to a greater extent than (NH4)2SO4. In the neutral soil, slurry additions resulted in the accumulation of NO2?-N and, in one case, the complete inhibition of nitrification for 4 weeks. Slurry raised the pH of both soils more than (NH4)2SO4 and nitrification in the acid soil was most rapid in a 2 week period of elevated pH following slurry applications. Numbers of Nitroxomonas isolated from the acid soil were considered high enough to account for NH4-N oxidation in slurry-treated samples. Numbers of nitrifiers recovered from the incubated neutral soil samples were variable but frequently high enough (>104/g dry soil) to account for observed rates of nitrification. Results are discussed in relation to heterotrophic nitrification in soils, and the practical implications of spreading slurry on agricultural land.  相似文献   

17.
无定形氧化铁作为嫌气下NH4+氧化时电子受体的研究   总被引：2，自引：0，他引：2       下载免费PDF全文
通过15N示踪试验,以及根据嫌气条件下含15NH4+培养液加无定形氧化铁后全氮回收量下降(损失15N约15%),以及产生15NO2、15N2O、15N14NO、15NO、15N2和15N14N等含N气体的研究结果,初步证明无定形氧化铁可作为嫌气下NH4+氧化时的电子受体,这可能是引起水田土壤中铵态氮肥损失的又一机理。目前因无各种合15N氧化物标准气体,无法对其形成的含15N气体组分进行定量,这一机理对氮素损失的贡献究竟有多大?尚需进一步研究。  相似文献   

18.
The co-application of glufosinate with nitrogen fertilizers may alter atrazine cometabolism, thereby extending the herbicide’s residual weed control in adapted soils. The objective of this study was to assess the effects of glufosinate, ammonium sulfate, and the combination of glufosinate and ammonium sulfate on atrazine mineralization in a Dundee silt loam exhibiting enhanced atrazine degradation. Application of glufosinate at rates of 10 to 40 mg kg−1 soil extended the lag phase 1 to 2 days and reduced the maximum degradation rate by 15% to 30%. However, cumulative atrazine mineralization averaged 85% 21 days after treatment and was independent of treatment. Maximum daily rates of atrazine mineralization were reduced from 41% to 55% by application of 1 to 8 g kg−1 of ammonium sulfate. Similarly, cumulative atrazine mineralization was inversely correlated with ammonium sulfate rates ranging from 1.0 to 8 g kg−1 soil. Under the conditions of this laboratory study, atrazine degradation was relatively insensitive to exogenous mineral nitrogen, in that 8 g (NH4)2SO4 per kilogram soil repressed but did not completely inhibit atrazine mineralization. Moreover, an additive effect on reducing atrazine mineralization was observed when glufosinate was co-applied with ammonium sulfate. In addition, ammonium fertilization alters the partitioning of 14C-atrazine metabolite accumulation and nonextractable residues, indicating that ammonium represses cleavage of the triazine ring. Consequently, results indicate that the co-application of glufosinate with N may increase atrazine persistence under field conditions thereby extending atrazine residual weed control in adapted soils.  相似文献   

19.
氮肥对镉在土壤-芥菜系统中迁移转化的影响   总被引：6，自引：0，他引：6  
以芥菜为研究对象, 采用盆栽试验, 探讨了不同用量的5种氮肥对污染农田土壤中镉(Cd)在土壤–根系–地上部迁移累积的影响。结果表明: 5种氮肥均促进了芥菜根系对Cd的吸收, 且根系Cd含量随施氮量的增加而增加; 但根系吸收转运Cd的能力随氮肥施用量的增加呈先降后增的变化趋势。在≤200 mg(N)·kg-1(土)的施氮水平下, CO(NH2)2和Ca(NO3)2处理能显著降低芥菜地上部Cd含量, 降低幅度分别为13%~29%和24%~30%。在施氮量相同的条件下, NH4Cl和(NH4)2SO4显著降低了土壤pH, 增加了土壤DTPA-Cd含量, 促进了芥菜对Cd的吸收。本试验条件下, 200 mg(N)·kg-1(土)的CO(NH2)2在增加芥菜产量和降低芥菜地上部Cd含量等方面优于其他氮肥处理。  相似文献   

20.
Field experiments were conducted to determine the effect of nitrogen (N) fertilizer forms and doses on wheat (Triticum aestivum L.) on three soils differing in their ammonium (NH4) fixation capacity [high = 161 mg fixed NH4-N kg?1 soil, medium = 31.5 mg fixed NH4-N kg?1 soil and no = nearly no fixed NH4-N kg?1 soil]. On high NH4+ fixing soil, 80 kg N ha?1 Urea+ ammonium nitrate [NH4NO3] or 240 kg N ha?1 ammonium sulfate [(NH4)2SO4]+(NH4)2SO4, was required to obtain the maximum yield. Urea + NH4NO3 generally showed the highest significance in respect to the agronomic efficiency of N fertilizers. In the non NH4+ fixing soil, 80 kg N ha?1 urea+NH4NO3 was enough to obtain high grain yield. The agronomic efficiency of N fertilizers was generally higher in the non NH4+ fixing soil than in the others. Grain protein was highly affected by NH4+ fixation capacities and N doses. Harvest index was affected by the NH4+ fixation capacity at the 1% significance level.  相似文献   

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