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1.
In wetland ecosystems, such as rice fields, methanogenic bacteria (MB) play important roles in global carbon cycling as terminal organic decomposers and in hydrogen cycling as hydrogen consumers, leading to methane production (Jorgensen 1982). In the global atmosphere, concentration of methane has been increasing by about 1% per year (Blake and Rowland 1986; Bouwman 1989; Dlugokensky et al. 1994) and it is considered that 80% is of biological origin (Seiler 1984). The environmental impact of methane on global warming has also been confirmed (Chappellaz et al. 1990). Rice paddy fields contribute to an estimated 10% of the global methane emission (Bouwman 1989; IPCC 1992) and the intensification of paddy cultivation may contribute considerably to the gradual increase of atmospheric methane (Rasmussen and Khalil 1981). From 1970 to 1990, rice production increased by 110% in West Africa (FAO 1970, 1990), as a direct result of encroachment on new lands throughout most of West Africa (Windmeijer and Andriesse 1993; Issaka et al. 1996a, b). In Burkina Faso, about 85% of the rice cultivation areas are lowlands (Sié 1991). In recent years, many studies on methane emission from paddy fields and limited ecological studies on methanogenic populations have been conducted (Schütz et al. 1989; Asakawa and Hayano 1995; Adachi et al. 1996). However, studies on both methane emission and populations of methanogens in African paddy fields are very limited. Garcia et al. (1974) enumerated methanogenic populations in Sénégal rice soils by the most probable number (MPN) method. With the gradual increase in the land area under rice cultivation, more information is needed on methane fluxes, populations, and species of methanogens in paddy fields of West Africa. We reported here the enumeration of methanogenic populations in the soils of lowland paddy fields located in the Sudan and Guinea Savanna zones of Burkina Faso (West Africa) by the MPN method.  相似文献   

2.
Rice‐straw amendment increased methane production by 3‐fold over that of unamended control. Application of P as single superphosphate at 100 μg (g soil)–1 inhibited methane (CH4) production distinctly in flooded alluvial rice soil, in the absence more than in the presence of rice straw. CH4 emission from rice plants (cv. IR72) from alluvial soil treated with single superphosphate as basal application, in the presence and absence of rice straw, and held under non‐flooded and flooded conditions showed distinct variations. CH4 emission from non‐flooded soil amended with rice straw was high and almost similar to that of flooded soil without rice‐straw amendment. The cumulative CH4 efflux was highest (1041 mg pot–1) in rice‐straw‐amended flooded soil. Appreciable methanogenic reactions in rice‐straw‐amended soils were evident under both flooded and non‐flooded conditions. Rice‐straw application substantially altered the balance between total aerobic and anaerobic microorganisms even in non‐flooded soil. The mitigating effects of single‐superphosphate application or low‐moisture regime on CH4 production and emission were almost nullified due to enhanced activities of methanogenic archaea in the presence of rice straw.  相似文献   

3.
Temporal variations in diversity of methanogenic community and CH4 production potential were analyzed in an Indian tropical rice ecosystem. Laboratory incubations showed that methane production varied from 20.86 to 134.11 μg CH4 g−1 d.w.s. during the two consecutive years, 2009 and 2010. CH4 production potential was high at the flowering stage of the rice crop followed by ripening, tillering, post-harvest and pre-plantation stage. Phylogenetic analysis of 16S rRNA genes of methanogenic community indicated that flowering and ripening stages comprised of Methanomicrobiaceae, Methanosarcinaceae, Methanosaetaceae and RC I methanogenic groups, while only the members of Methanomicrobiaceae and RC I were present in the remaining stages. Further, the dominance of RC I was observed in all stages. This study demonstrates that flowering and ripening stages of rice crop offer relatively favorable ecological niche for methanogenic community. The overall analyses suggest that the temporal change in diversity of methanogens regulates CH4 production potential in rice field soils.  相似文献   

4.
Abstract

Methane (CH4) is one of the most abundant organic gases in the atmosphere. Recently the importance of CH4 as a greenhouse gas has been recognized and studies have been carried out to assess its contribution to global warming. Although the rate of increase has slowed down in the last decade (Steel et al. 1992; Rudolph 1994), the results from some of these studies have shown that the atmospheric concentration of CH4 is increasing at a rate estimated to be approximately 1% per year (Rowland 1991; Blake and Rowland 1988; Bolle et al. 1986; Graedel and McRae 1980). Clearly it is important to identify sources and sinks of CH4, in both terrestrial and oceanic ecosystems, in order to estimate global methane budgets (Cicerone and Oremland 1988).  相似文献   

5.
Addition of rice straw, which is a common practice in rice agriculture, generally results in enhanced production and emission of the greenhouse gas methane (CH4). However, it is unclear whether straw addition affects only the activity or also the composition of the methanogenic microbial community. It is also unclear to what extent methanogenic archaea would be able to proliferate in the soil. Anoxic slurries of Italian rice‐field soil produced CH4 after a lag, during which ferric iron and sulfate were reduced. Addition of rice straw slightly decreased this lag and greatly enhanced the subsequent production of CH4. At the same time, addition of rice straw enhanced the intermediate production of H2 and acetate that served as the methanogenic substrates. Compared with the unamended control, the addition of rice straw resulted in an increased concentration of phospholipid fatty acids in the soil. Quantitative ‘real‐time’ PCR targeting the 16S rRNA gene also showed increased copy numbers of both Bacteria and Archaea in the straw‐amended soil at the end of the experiment. The composition of the archaeal community was followed over time by terminal restriction length polymorphism (T‐RFLP) analysis of the archaeal 16S rRNA genes extracted from straw‐amended soil and the control. Rice Cluster‐I (RC‐I) methanogens and Methanosarcinaceae were the most abundant methanogenic populations, followed by Methanobacteriales, Methanomicrobiales and Methanosaetaceae. Addition of rice straw resulted in a relative increase of Methanosarcinaceae and Methanobacteriales and a relative decrease of RC‐I methanogens and Methanomicrobiales. Our results revealed a dynamic methanogenic community in anoxic rice‐field soil and showed that addition of organic matter selectively enhanced the growth of particular methanogenic populations, which were apparently better adapted to the presence of straw than the others. The extent of archaeal growth was consistent with that expected theoretically from the ambient Gibbs free energies of hydrogenotrophic and acetoclastic methanogenesis.  相似文献   

6.
间隙灌溉和控释肥施用对稻田土壤产甲烷微生物的影响   总被引：1，自引：0，他引：1  
纪洋  于海洋  Conrad Ralf  徐华 《土壤》2017,49(6):1132-1139
间隙灌溉和控释肥施用影响稻田CH_4的产生和排放,然而其微生物机理尚不清楚。本研究通过采集稻季田间原位试验新鲜土样,采用核酸定量技术(qPCR)和末端限制性片段长度多态性(T-RFLP)技术,研究间隙灌溉和控释肥施用对稻田土壤产甲烷微生物群落丰度和结构的影响。结果表明,稻季CH_4排放量与古菌、产甲烷菌(mcr A基因)和甲烷氧化菌(pmo A基因)数量均呈极显著正相关关系(P0.01),而与细菌数量无显著相关性。间隙灌溉显著影响产甲烷菌和甲烷氧化菌数量的季节变化,其中烤田抑制产甲烷菌生长,而对甲烷氧化菌数量没有显著影响。与尿素相比,施用控释肥增加了稻田土壤细菌、古菌和产甲烷菌数量,降低了甲烷氧化菌数量。土壤古菌群落的优势T-RFs片段为184bp和391bp,其中184bp片段的相对丰度随着间隙灌溉的进行由45%~55%降低到23%~30%;而391bp片段则相反,其相对丰度由12%~18%增加到23%~26%。典型相关性分析(CCA)表明间隙灌溉显著影响土壤古菌群落结构(P0.001),而控释肥施用对土壤古菌群落结构没有明显影响。  相似文献   

7.
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8.
With an understanding of the processes of methane production, oxidation and emission, a semi-empirical model, focused on the contributions of rice plants to the processes and also the influence of environmental factors, was developed to predict methane emission from rice paddy soils. In the present model, the amount of methane transported from the soil to the atmosphere was determined by the rates of CH4 production and an emitted fraction. The rates of CH4 production in irrigated rice soils were computed from the availability of methanogenic substrates that are primarily derived from rice plants and added organic matter and the influence of soil texture, soil redox potential and temperature. The fraction of methane emitted was assumed to be modulated by the rice plants and declines with rice growth and development. To make it applicable to a wider area with limited data sets, a simplified version of the model was also derived to predict methane emission in a more practical manner.  相似文献   

9.
中国常年淹水稻田CH4排放量估算   总被引：4，自引：0，他引：4  
A special kind of rice field exists in China that is flooded year-round. These rice fields have substantially large CH4 emissions during the rice-growing season and emit CH4 continuously in the non-rice growing season. CH4 emission factors were used to estimate the CH4 emissions from year-round flooded rice fields during the rice-growing season in China.The CH4 emissions for the year-round flooded rice fields in China for the rice growing season over a total area of 2.66 Mha were estimated to be 2.44 Tg CH4 year^-1. The uncertainties of these estimations are discussed as well. However,the emissions during the non-rice growing season could not be estimated because of limited available data. Nevertheless,methane emissions from rice fields that were flooded year-round could be several times higher than those from the rice fields drained in the non-rice-growing season. Thus, the classification of “continuously flooded rice fields”in the IPCC (International Panel on Climate Change) Guidelines for National Greenhouse Gas Inventories is suggested to be revised and divided into “continuously flooded rice fields during the rice growing season” and “year-round flooded rice fields”.  相似文献   

10.
In relation to global warming, a great deal of attention has been paid to methane (CH4 ) emission from paddy fields. The amount of CH4 emitted from paddy fields is now estimated to account for about 12% of the total CH4 emission according to Prather et al. (1995). Harvested area of rough rice in Asia covered 1,320,000 km2 in 1990 and 38% of the area was estimated to be maintained under rainfed conditions (IRRI 1991).  相似文献   

11.
Ralf CONRAD 《土壤圈》2020,30(1):25-39
Microbial methanogenesis is a major source of the greenhouse gas methane(CH4).It is the final step in the anaerobic degradation of organic matter when inorganic electron acceptors such as nitrate,ferric iron,or sulfate have been depleted.Knowledge of this degradation pathway is important for the creation of mechanistic models,prediction of future CH4 emission scenarios,and development of mitigation strategies.In most anoxic environments,CH4 is produced from either acetate(aceticlastic methanogenesis)or hydrogen(H2)plus carbon dioxide(CO2)(hydrogenotrophic methanogenesis).Hydrogen can be replaced by other CO2-type methanogenesis,using formate,carbon monoxide(CO),or alcohols as substrates.The ratio of these two pathways is tightly constrained by the stoichiometry of conversion processes.If the degradation of organic matter is complete(e.g.,degradation of straw in rice paddies),then fermentation eventually results in production of acetate and H2 at a ratio of>67%aceticlastic and<33%hydrogenotrophic methanogensis.However,acetate production can be favored when heterotrophic or chemolithotrophic acetogenesis is enhanced,and H2 production can be favored when syntrophic acetate oxidation is enhanced.This typically occurs at low and elevated temperatures,respectively.Thus,temperature can strongly influence the methanogenic pathway,which may range from 100%aceticlastic methanogenesis at low temperatures to 100%hydrogenotrophic methanogenesis at high temperatures.However,if the degradation of organic matter is not complete(e.g.,degradation of soil organic matter),the stoichiometry of fermentation is not tightly constrained,resulting,for example,in the preferential production of H2,followed by hydrogenotrophic methanogenesis.Preferential production of CH4 by either aceticlastic or hydrogenotrophic methanogenesis can also happen if one of the methanogenic substrates is not consumed by methanogens but is,instead,accumulated,volatilized,or utilized otherwise.Methylotrophic methanogens,which can use methanol as a substrate,are widespread,but it is unlikely that methanol is produced in similar quantities as acetate,CO2,and H2.Methylotrophic methanogenesis is important in saline environments,where compatible solutes are degraded to methyl compounds(trimethyl amine and dimethyl sulfide)and then serve as non-competitive substrates,while acetate and hydrogen are degraded by non-methanogenic processes,e.g.,sulfate reduction.  相似文献   

12.
烤田对种稻土壤甲烷排放的影响   总被引：20，自引：0，他引：20  
本文通过温室盆栽试验研究了烤田对种稻土壤甲烷排放的影响。在水稻移栽后的第４３天和１０２天各烤田一次,持续时间分别为２８和１１３小时。结果表明种稻土壤在开台田后前１６和４２小时内有大量甲烷排放,且在烤田后不久土壤呈微干松软状态时出现甲烷排放高峰。随着烤田的延续,至土壤呈干裂状态时甲烷排放通量降为零,烤田能促进土壤闭蓄态甲烷的排放,烤田期间甲烷排放量点水稻生长期甲烷排放总量的５．９６％～１０．０５％。  相似文献   

13.
 Application of a commercial formulation of the herbicide butachlor (N-butoxymethyl-2-chloro-2′,6′-diethyl acetanilide) at 1 kg a.i. ha–1 to an alluvial soil planted with direct-seeded flooded rice (cv. Annada), significantly inhibited both crop-mediated emission and ebullition fluxes of methane (CH4). Over a cropping period of 110 days, the crop-mediated cumulative emission flux of CH4 was lowered by ∼20% in butachlor-treated field plots compared with that of an untreated control. Concurrently, ebollition flux of CH4 was also retarded in butachlor-treated field plots by about 81% compared with that of control plots. Significant relationships existed between CH4 emission and redox potential (E h) and Fe2+ content of the flooded soil. Application of butachlor retarded a drop in soil redox potential as well as accumulation of Fe2+ in treated field plots. Methanogenic bacterial population, counted at the maturity stage of the crop, was also low in butachlor-treated plots, indicating both direct and indirect inhibitory effects of butachlor on methanogenic bacterial populations and their activity. Results indicate that butachlor, even at field-application level, can effectively abate CH4 emission and ebollition from flooded soils planted to rice whilst maintaining grain yield. Received: 15 March 2000  相似文献   

14.
In a field study, potassium (K) applied as muriate of potash (MOP) significantly reduced methane (CH4) emission from a flooded alluvial soil planted to rice. Cumulative emission was highest in control plots (125.34 kg CH4 ha−1), while the lowest emission was recorded in field plots receiving 30 kg K ha−1 (63.81 kg CH4 ha−1), with a 49% reduction in CH4 emission. Potassium application prevented a drop in the redox potential and reduced the contents of active reducing substances and Fe2+ content in the rhizosphere soil. Potassium amendment also inhibited methanogenic bacteria and stimulated methanotrophic bacterial population. Results suggest that, apart form producing higher plant biomass (both above- and underground) and grain yield, K amendment can effectively reduce CH4 emission from flooded soil and could be developed into an effective mitigation option, especially in K-deficient soils.  相似文献   

15.
Soil characteristics regulate various belowground microbial processes including methanogenesis and, consequently, affect the structure and function of methanogenic archaeal communities due to change in soil type which in turn influences the CH4 production potential of soils. Thus, five different soil orders (Alfisol, Entisol, Inceptisol, Podzol and Vertisol) were studied to assess their CH4 production potential and also the methanogenic archaeal community structure in dryland irrigated Indian paddy soils. Soil incubation experiments revealed CH4 production to range from 178.4 to 431.2 μg CH4 g-1 dws in all soil orders as: Vertisol<Inceptisol<Entisol<Podzol<Alfisol. The numbers of methanogens as quantified using real-time quantitative polymerase chain reaction (qPCR) targeting mcrA genes varied between 0.06 and 72.97 (×106 copies g-1 dws) and were the highest in Vertisol soil and the least in Alfisol soil. PCR-denaturing gradient gel electrophoresis (DGGE)-based approach targeting 16S rRNA genes revealed diverse methanogenic archaeal communities across all soils. A total of 43 DGGE bands sequenced showed the closely related groups to Methanomicrobiaceae, Methanobacteriaceae, Methanocellales, Methanosarcinaceae, Methanosaetaceae and Crenarchaeota. The composition of methanogenic groups differed among all soils and only the Methanocellales group was common and dominant in all types of soils. The highest diversity of methanogens was found in Inceptisol and Vertisol soils. Methane production potential varied significantly in different soil orders with a positive relationship (p?<?0.05) with methanogens population size, permanganate oxidizable C (POXC) and CO2 production. The present study suggested that CH4 production potential of different soils depends on physicochemical properties, methanogenic archaeal community composition and the population size.  相似文献   

16.
Estimation of release rates of CH4, N2O, and other trace gases from rice agriculture in China requires that details of microbiology, rice field benthic fauna, fertilizer use, and how farmers farm be understood. The potential importance of natural fertilizer can be estimated, based on published biogas generator efficiency in rural China, with the result that CH4 production from the fermentation of animal and human wastes prior to use as fertilizer may be comparable to that expected to be released from flooded rice fields. A review of agricultural practices in Anhui and Fujian Provinces indicates that marked seasonal and geographic variations in CH4 and other trace gas releases to the atmosphere from Chinese agricultural areas are expected.  相似文献   

17.
Rice straw polysaccharides are one of the major C sources for CH4 formation in anoxic rice paddy soils. We investigated the initial step of straw degradation by measuring the substrate-saturated activities of the polysaccharolytic enzymes #-glucosidase, exo-#-1,4-glucanase and xylosidase using substrates labelled with methylumbelliferone (MUF). The actual activity of the enzymes was measured by the release of reducing sugars after the inhibition of microbial carbohydrate uptake by toluene. The substrate-saturated enzyme activities increased during the first 11 days of incubation, while the actual activities decreased, presumably due to the decreasing access of straw polysaccharides to hydrolytic enzymes. The temporal progress of polysaccharide hydrolysis, transient accumulation of fermentation products and CH4 production indicated five distinct phases. In phase I (<8 h), the fermentation of sugar monomers released by hydrolysis of polysaccharides was limiting. In phase II (2 and acetate accumulated since the activity of methanogens was low, though increasing exponentially. In phase III (days 3-10), H2 was also consumed by respiratory processes (e.g. SO42- reduction) so that H2-dependent methanogenesis sometimes became substrate limited. However, acetate still accumulated, probably due to the limiting activity of acetoclastic methanogens. In phase IV (days 10-18), methanogenic activity was no longer limited and acetate was depleted to low concentrations. In phase V (>day 18), the methanogenic degradation of straw reached a quasi-steady state, when polysaccharide hydrolysis became the rate-limiting step for CH4 formation.  相似文献   

18.
Methane emission from flooded rice fields under irrigated conditions   总被引：11，自引：0，他引：11  
In a study on CH4 emission from flooded rice fields under irrigated conditions, fields planted with rice emitted more methane than unplanted fields. The CH4 efflux in planted plots varied with the rice variety and growth stage and ranged from 4 to 26 mg h-1m-2. During the reproductive stage of the rice plants, CH4 emission was high and the oxidation power of rice roots, in terms of -naphthylamine oxidation, was very low. The CH4 emission reached a maximum at midday and declined to minimum levels at midnight, irrespective of the rice variety. The peak CH4 emission at midday was associated with higher solar radiation and higher soil/water temperature.  相似文献   

19.
Cultivation of green manure plants during the fallow season in rice paddy soil has been strongly recommended to improve soil properties. However, green manuring may impact greenhouse gas emission, methane (hereafter, CH4) in particular, under the flooded rice cultivation and thus, application of chemical amendments being electron acceptors can be an effective mitigation strategy to reduce CH4 emissions in irrigated rice (Oryza sativa L.) field amended with green manure. To investigate the effect of iron (Fe) slag silicate fertilizer (hereafter, silicate fertilizer), which was effective in reducing CH4 emission and increasing rice productivity, in green manure-amended paddy soil, the aboveground biomass of Chinese milk vetch (hereafter, vetch) was added at rates of 0, 10, 20, and 40 Mg (fresh weight) ha−1 before the application of silicate fertilizer, which was added at rates of 0 and 2.3 Mg ha−1. Silicate fertilization reduced the seasonal CH4 flux by ca. 14.5% and increased rice yield by ca. 15.7% in the control (no vetch application) treatment. However, CH4 production was increased by silicate fertilization in vetch-treated soil particularly at the initial rice growing stage, which was probably due to the enhanced decomposition of added organic matters by the silicate liming effect. In conclusion, silicate fertilization is not effective in reducing CH4 production in green manure-amended rice paddy soils and its use should be properly controlled.  相似文献   

20.
Pot experiments were conducted to monitor the changes in compositions and δ13C values of soil-trapped CH4 and C02 in flooded rice soil with and without rice plants or rice straw. Incorporation of rice straw increased the concentration of CH4 and C02 accumulated in soil, and the quantities of emitted CH4 to the atmosphere. Rice plants reduced the concentration of soil-trapped CH4 and CO2, and the decreased portion of CH4 was replaced by N2. A significantly negative correlation was found between soil-trapped CH4 and N2. The presence of rice plants increased the δ13C values of CH4. The δ13C values of CH4 tended to increase toward the end of the growing season and were positively correlated with concentration of soil-trapped CH4. A positive correlation between δ13C values of CH4 and C02, and between the δ13C values of CH4 and its concentration, were observed. The CH4 in the rice stems was 4–14% enriched in13C relative to soil-trapped CH4. In contrast, CO2 in rice plant stems was 1–9% lighter in13C relative to soil-trapped C02. These results are discussed in relation to the precursor pools and pathways of methanogenesis.  相似文献   

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