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1.
槐耳菌液体培养富硒的研究 总被引：1，自引：0，他引：1
当发酵培养基中的硒浓度达到20μg/mL时。对菌丝体生长的抑制率最小，多糖含量达到51．3mg／g。同时进行了槐耳菌富硒条件的优化：pH5．5，接种量10％。装液量80mL/500mL三角瓶。28℃。200r／min发酵培养9d。且在发酵第5d加入硒溶液。槐耳多糖含量达到50．3mg／g，多糖中硒含量达到761．9μg／g，多糖对硒的转化率达到35．8％。 相似文献
2.
主要研究了红栓菌和槐耳菌对硒的耐受力以及菌丝体蛋白对硒的富集作用。结果表明．红栓菌的生长速率明显要快于槐耳菌．蛋白质富集硒的能力比槐耳菌强．但对硒的敏感性较强。当发酵培养基中硒的添加量达到25μg／ml时．对菌丝体生长的抑制率最小。且在发酵初期添加无机硒．红栓菌蛋白质富硒能力最强．其中蛋白总硒量为255.87μg．蛋白质中硒含量为6．40μg／mg。 相似文献
3.
虫草液体深层发酵富硒的研究 总被引：5，自引：0，他引：5
研究了不同硒浓度、培养时间、pH、培养基种类等因素对虫草菌丝体生长量、富硒量、有机硒转化率的影响，探讨了虫草菌富硒的最优培养条件。结果表明其最优富硒培养条件为：培养基硒浓度60μg／mL，培养时间5d，pH6，培养基为麸皮培养基（％）：蔗糖3，麸皮2，NaN030．2，KH2V040．5。菌丝生长量最高可达1．916g/100mL，有机硒含量可达509．026μg／100mL。虫草有机硒转化率最佳培养条件为：培养基硒浓度20μg／mL，培养时间5d，pH7，培养基为马铃薯培养基（％）：蔗糖3，马铃薯20，NaNCh0．2，KH2PO40．5。其有机硒转化率最高可达18．44％。低浓度的硒可以刺激菌丝体生长，高浓度的硒对菌丝体生长有抑制作用，使有机硒转化率降低。 相似文献
4.
茶树菇菌株富硒生长及营养成分分析研究 总被引：1，自引：0，他引：1
比较了五种茶树菇菌株富硒生长的状况及营养成分分析。结果表明，野生种和As-1在耐硒和蛋白质及多糖的合成上都优于其他3个菌株。2μg／mL和5μg／mL硒浓度对5种茶树菇菌株的生长有一定的促进作用。5μg／mL硒浓度中的菌丝生物量和蛋白质、多糖的含量达最高值，野生种和As-1的蛋白质含量分别达022mg／mL和0．23mg／mL，多糖含量分别达038mg／mL和0．41mg／mL。硒浓度超过10μg／mL，对各菌株的生长表现为抑制，当硒浓度大于50μg／mL，时，除野生种外所有菌株停止生长。野生种具有较高的耐硒能力，在硒质量浓度在50μg／mL的情况下依然能够生长。 相似文献
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蛹虫草菌丝体循环富硒法的建立及其硒多糖抑癌作用初探 总被引：1，自引：0，他引：1
研究富硒蛹虫草菌丝体循环富硒作用及该法获取的硒多糖对人宫颈癌细胞Hela的抑制作用.采用循环富硒法获得蛹虫草富硒菌丝体并制备硒多糖,经硒定量和多糖含量测定后采用MTT.法检测硒多糖与正常蛹虫草多糖(12.5、25、50、100、200μg/mL)作用Hela细胞72 h后细胞存活率的变化.循环富硒法可有效促进蛹虫草菌丝体对硒的富集,硒含量为680.2μg/g,多糖含量为47.4%;硒多糖与正常蛹虫草多糖(100μg/mL)对Hela细胞的抑制率分别为91.87%和77.98%.其IC50分别为18.5μg/mL和48.5μg/mL.循环富硒法可有效制备富硒蛹虫草菌丝体和硒多糖,循环富硒法获取的硒多糖能有效的抑制Hela细胞的增殖. 相似文献
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7.
平菇菌丝富硒培养条件的优化研究 总被引：1，自引：0，他引：1
对平菇菌丝富硒培养进行单因子试验，在此基础上进行了富硒发酵条件的正交试验研究。结果表明：葡萄糖质量分数1．5％，亚硒酸钠质量浓度8．0mg／L，起始pH7．0，培养时间5d，平菇菌丝富硒量得到最大，为331．49μg／g菌丝。 相似文献
8.
杏鲍菇不同菌株生物学特性的研究 总被引：6，自引：2，他引：6
比较研究了杏鲍菇4个菌株的菌丝拮抗性、酯酶同工酶酶谱、菌丝生长速度、菌丝体生物量及多糖含量。结果表明，4个菌株问存在遗传差异。菌株X3菌丝生长速度最快，日均长速为0．69cm；菌株X2菌丝体生物量最高，为1．572g／100mL；菌株X1菌丝体多糖含量最低，仅为39.07μg／mg，菌株X2、X4和X3菌丝体多糖含量分别为84．28μg/mg、79．20μg／mg和78．99μg/mg。 相似文献
9.
研究了稀土元素镧（La）对灵芝菌丝体富集有机硒的影响。结果表明,镧能有效促进灵芝菌丝富集有机硒,当发酵培养基中镧的添加浓度为100mg／L时,菌丝产量和硒转化率最高。正交试验结果表明,各因素中对灵芝菌丝体富硒影响最大的是镧浓度,其次是硒浓度、培养时间和pH值,最佳发酵条件是镧浓度100mg／L,硒浓度100mg／L,pH值4．5,培养时间7d,在此条件下菌丝体产量47．3g／L,硒转化率88．1％。 相似文献
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Zusammenfassung Die Leistungsprüfungen wurden im Zeitraum 1997 bis 2003 mit den Unterlagen Gisela 4 und 5, den Klonnummern 195/20 und 497/8 aus der Gisela-Serie sowie Weiroot 10, 13, 53, 72 und 158 durchgeführt. Dabei dienten Sämlinge von P1 (bulgarische Selektion aus Prunus mahaleb) als Kontrolle. Alle Unterlagen waren mit der Sorte Stella veredelt und im Dezember 1996 in der Versuchsanlage der Agraruniversität in Plovdiv, Bulgarien, im Abstand von 6 m×4,5 m gepflanzt worden. Dabei erfolgte ein Pflanzschnitt. Nach Abschluss der natürlichen Kronenentwicklung wurde jedes Jahr ein Winterschnitt vorgenommen. Der Boden wurde durch mechanische Bearbeitung offen gehalten und nach dem 4. Standjahr wurden die Baumstreifen mit Herbiziden behandelt. Die Wasserversorgung erfolgte durch eine dem natürlichen Gefälle folgende Überflutung, allerdings nicht immer zum optimalen Zeitpunkt, da keine eigene Wasserquelle zur Verfügung stand.Basierend auf den Ergebnissen bis zum Anfang des 7. Standjahres können die untersuchten Unterlagen in zwei Gruppen differenziert werden: starkwüchsig—Weiroot 10, P1 und Weiroot 13; mittelstarkwachsend bis schwachwüchsig—Gi 497/8, Gisela 4, Weiroot 53, Weiroot 158, Gi 195/20, Weiroot 72 und Gisela 5. Letztere zeichnete sich durch besondere Schwachwüchsigkeit aus. Die meisten Wurzelschosser bildeten Gisela 4, Weiroot 10 und Weiroot 13. Weiroot 53, Weiroot 72 und Weiroot 158 entwickelten deutlich weniger und P1, Gisela 5, Gi 195/20 sowie Gi 497/8 keine Wurzelschosser. Den frühesten Blühbeginn induzierte Gisela 4. Die anderen Unterlagen führten, in Abhängigkeit von den Temperaturbedingungen des jeweiligen Jahres, zu einer Verspätung der Blüte: P1 und Weiroot 10 um 1–2 Tage; Gi 497/8, Weiroot 13 und Weiroot 158 um 2–4 Tage; Weiroot 72 um 2–7 Tage; Gi 195/20 um 3–6 Tage; Weiroot 53 um 3–8 Tage und Gisela 5 um 3–10 Tage. Die Reifezeit der Früchte war bei den Bäumen auf Gisela 5 im Vergleich zu den anderen Varianten um 2–3 Tage verspätet. Gisela 5, Weiroot 72 und Gisela 4 induzierten bei der aufveredelten Sorte die höchsten Ertragsleistungen, P1 die geringsten. Bei den Bäumen auf Gisela 5 war die Fruchtgröße geringer als bei den anderen Unterlagen. Bäume auf Gisela 5 brauchen intensive Pflege. Nur wenn alle Produktionsfaktoren und kulturtechnischen Maßnahmen optimiert werden, kann das hohe Ertragspotenzial dieser Unterlage ausgeschöpft werden. 相似文献
12.
AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β-particles emission from 188Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by 188Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [3H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of 188Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from 188 Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G0/G1 arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation. 相似文献
13.
AIM:To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS:After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly (P＜0.01). The ET content in plasma also decreased significantly by L-Arg(P＜0.01).CONCLUSION: Plasma ET content and the expression of c-fos in the left ventricle of rats with renovascular hypertensive hypertrophy could be decreased by L-Arg administration. 相似文献
14.
GONG Yong-sheng LI Ji-wu YANG Peng-lin FAN Xiao-fang HU Liang-gang ZHENG Lü-zhen JIANG Zhong-sun 《园艺学报》2003,19(9):1261-1264
AIM:To investigate the effect of puerarin on pulmonary vessel collagen metabolism in pulmonary hypertension rats induced by chronic hypoxia and hypercapnia.METHODS:Collagen Ⅰ, Ⅲ and their mRNA were observed in pulmonary arterioles by the technique of immunohistochemistry and in situ hybridization.RESULTS:① Light microscopy showed media thickness of pulmonary arterioles was much higher in HH(hypoxic-hypercapnia) group than that of NC(normal control) group, and, vessel cavity turned more straiter in HH group than that of NC group.However, the damage of pulmonary arterioles in HP(hypoxic-pueratin) group was much slighter than that of HH group. ② The levels of plasma ET-1 and lung homogenates Hyr were much higher in HH group than those of NC group(P＜0.01), and lower in HP group than HH groups(P＜0.01).Plasma NO content in group HH was lower than that of group NC(P＜0.01), it was higher in group HP than that of group HH(P＜0.01).③Expression of collagen Ⅰ and collagen Ⅰ mRNA in pulmonary arterioles were significantly higher in HH groups than those of NC group (P＜0.01), and they were lower in HP group than those of HH group (P＜0.01).Expression of collagen Ⅲ and collagen Ⅲ mRNA showed no difference among three groups(P＞0.05).CONCLUSION:Puerarin inhibited the deposition of collagen and improved pulmonary vessel remodeling. 相似文献
15.
AIM: To examine the autoantibody against α1-adrenoceptor and its biologic activities during the development of renal hypertension. METHODS: Renal hypertension of rat was achieved by clipped renal artery, the titre of autoantibody to α1-adrenoceptor was detected using ELISA immunoassay. Furthermore, the biological offects of these autoantibodies on cultured cardiomyocytes were also examined. RESULTS: After two weeks of clipping renal arteries, both the frequency of occurrence and the titre of autoantibodies to cardiac α1-adrenergic receptor were significantly increased as compared with the control of pre-treatment. The increased autoantibodies lasted for several weeks and then automatically decreased gradually to the pre-clipping level at 12 weeks. The biological effects of these autoantibodies displayed an "agonistic-like" activities on the beating frequency of cultured neonatal cardiomyocytes. CONCLUSION: Autoantibodies against α1-adrenoceptor may play a role in the elevation of peripheral vascular resistance and in the development of cardiac hypertrophy in rats with renal hypertension. 相似文献
16.
《International Journal of Fruit Science》2013,13(3):101-120
Abstract Saskatoon berry (Amelanchier alnifolia Nutt., Rosaceae) and blueberry (Vaccinium corymbosum L., Ericaceae) are substantially equivalent in all characteristics that are important to the consumer, including fruit color, shape, size, nutrition, texture, and uses. In addition, both fruits are native to North America and they have practically identical historical uses and known health benefits. Their composition, processing, nutritional value and metabolism, intended uses, and levels of undesirable substances are compared. 相似文献
17.
《International Journal of Fruit Science》2013,13(1):139-156
Abstract This review is based partly on complete articles and partly on abstracts. Three of the 60 articles deal with the total uptake of elements in strawberry plant organs in two different strawberry production systems, both considered as optimal concerning amount and balance of elements. The effect on fruit quality may be dramatic if the level of a particular element is outside this range, but there may also be effects initiated by differences within the optimal range of elements. Most articles refer to product oriented quality, but some focus on consumer oriented quality, as discussed by Shewfelt (1999). The discussion here is on a general basis, so one should keep in mind that there are cultivar differences and that specification of nutrition ideally should mirror the needs of a single cultivar, or a group of cultivars with similar requirements. Also, to get a complete understanding of the subject future reviews should embrace a broader access of information including the effect on plant development of individual elements, such as the role of calcium in fruit firmness and its importance in cell wall structure. However, the intention here is to narrow the information to results that suggest a direct connection between nutrient uptake and fruit quality. 相似文献
18.
Habitat for wide-ranging species should be addressed at multiple scales to fully understand factors that limit populations.
The marbled murrelet (Brachyramphus marmoratus), a threatened seabird, forages on the ocean and nests inland in large trees. We developed statistical relationships between
murrelet use (occupancy and abundance) and habitat variables quantified across many spatial scales (statewide to local) and
two time periods in California and southern Oregon, USA. We also addressed (1) if old-growth forest fragmentation was negatively
associated with murrelet use, and (2) if some nesting areas are more important than others due to their proximity to high
quality marine habitat. Most landscapes used for nesting were restricted to low elevation areas with frequent fog. Birds were
most abundant in unfragmented old-growth forests located within a matrix of mature second-growth forest. Murrelets were less
likely to occupy old-growth habitat if it was isolated (> 5 km) from other nesting murrelets. We found a time lag in response
to fragmentation, where at least a few years were required before birds abandoned fragmented forests. Compared to landscapes
with little tono murrelet use, landscapes with many murrelets were closer to the ocean's bays, river mouths, sandy shores,
submarine canyons, and marine waters with consistently high primary productivity. Within local landscapes (≤ 800ha), inland
factors limited bird abundance, but at the broadest landscape scale studied (3200 ha), proximity to marine habitat was most
limiting. Management should focus on protecting or creating large, contiguous old-growth forest stands, especially in low-elevation
areas near productive marine habitat.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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The objective of this study was to establish a cryopreservation protocol for hawthorn shoot apices (Crataegus pinnatifida Bge.). Cryopreservation was carried out via encapsulation–dehydration, vitrification, and encapsulation–vitrification on shoot apices excised from in vitro cultures. We began by showing that cold-acclimation enhanced the regrowth of cryopreserved apices from 10.0 to 65.5% in encapsulation–dehydration. We then decided that the encapsulation–dehydration method was an optimal cryopreservation method for hawthorn shoot apices in terms of its high recovery after cryopreservation as well as its ease of use compared with vitrification and encapsulation–vitrification. In encapsulation–dehydration, the protocol leading to optimal regrowth was as follows: after cold-acclimation at 5 °C in the dark for 2 weeks, excised shoot tips were pretreated for 24 h at 25 °C on hormone-free Murashige and Skoog [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497] (MS) basal medium with 0.4 mol/L sucrose, then encapsulated and precultured in liquid MS medium with 0.8 mol/L sucrose for 16 h at 25 °C. Precultured beads were dehydrated for 6 h at 25 °C in the dessicator containing 50 g silica gel to a moisture content of 15.3% (fresh-weight basis) before cryostorage for 1 h. In addition, we examined the effect of adding glycerol to both the alginate beads and loading solution to enhance regrowth after cryopreservation in encapsulation–dehydration. In the present study, it was shown that adding 0.5 mol/L glycerol resulted in high regrowth percentages (82.5–90.0%) in four Crataegus species. 相似文献
20.
AIM: To observe the effect of thichosanthes injection on the expression of proliferating cell nuclear antigen (PCNA) of vascular smooth muscle cell (SMC). METHODS: The expression of PCNA of cultured rabbit aortic SMC was examined with LSAB immunohistochemical technique, and [3H]-thymidine( [3H]-TdR) incorporation data of SMC and the contents of superoxide dismutase (SOD), lipid peroxide (LPO), prostacyclin (PGI2) and cyclic AMP (cAMP) in medium were simultaneously determined. RESULTS: Thichosanthes injection has an effects of increasing SOD activity, decreasing LPO, elevating PGI2 and cAMP, reducing [3H]-TdR incorporation and expression of PCNA (all P＜0.05,P＜0.01). CONCLUSION: Thichosanthes could inhibit SMC proliferation. 相似文献