共查询到18条相似文献，搜索用时 78 毫秒
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为研究喜树碱(CPT)对小鼠成纤维细胞NIH/3T3中Toll样受体3(TLR3)的表达及活化的影响,本实验利用CCK-8试剂盒测定了CPT在不同浓度下、不同作用时间对NIH/3T3细胞的毒性作用,并确定CPT对NIH/3T3细胞的基本无毒浓度为1μg/mL。通过流式细胞术检测CPT作用NIH/3T3细胞表明,TLR3平均荧光强度与对照组相比显著增强,表明TLR3表达增加。此外,ELISA试剂盒检测结果显示CPT作用后IFN-β表达量增加,并高于阳性对照组,差异极显著(p<0.01)。本研究表明CPT对NIH/3T3细胞的TLR3具有显著的激动作用。 相似文献
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《中国兽医学报》2016,(5):880-884
本试验利用RNAi方法抑制牛卵丘细胞中JARID2基因mRNA的表达水平,通过免疫荧光方法检测H3K4me3、H3K9me3、H3K27me3、H3K36me3的甲基化程度。结果表明,在牛卵丘细胞中存在JARID2的表达,并且存在H3K9me3、H3K27me3的甲基化修饰,但没有检测到H3K4me3、H3K36me3的甲基化修饰。通过siRNA对牛卵丘细胞中JARID2 mRNA的表达进行成功抑制后,转染组JARID2的表达量明显下降,但H3K4me3、H3K27me3表达量无明显变化,而H3K9me3、H3K27me3表达量明显下降,即JARID2在mRNA水平上能够影响H3K9me3、H3K27me3的甲基化修饰程度,而对H3K4me3、H3K36me3的甲基化修饰无明显作用。 相似文献
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《畜牧与兽医》2015,(5):31-37
14-3-3蛋白在哺乳动物中均有表达,是一类高度保守的酸性蛋白家族,广泛参与各种信号传导途径,具有十分重要的功能。本研究以猪的14-3-3蛋白家族为研究对象,运用生物信息学方法,首先对猪(Sus scrofa)14-3-3蛋白家族基因组数据库进行搜素,获得猪14-3-3蛋白家族的7个亚型。然后通过对所有的猪14-3-3蛋白家族的DNA序列及各种表达序列标签(Expression Sequence Tags,ESTs)作进一步比对分析。结果表明:猪14-3-3蛋白家族的7个亚型在猪中均有表达;蛋白质多序列匹配分析表明猪14-3-3蛋白存在多个保守结构域,在各功能区段相当保守;通过基因结构分析、ESTs表达分析和蛋白质预测分析表明,14-3-3蛋白质家族均为亲水性蛋白,且不同亚型在猪体内不同组织表达,参与不同的生物学功能。本文将为猪14-3-3蛋白家族进一步的功能分析提供研究基础。 相似文献
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Xanthophylls increased HDLC level and nuclear factor PPARγ, RXRγ and RARα expression in hens and chicks 
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下载免费PDF全文 Y.‐Y. Gao L. Jin H. Peng L.‐H. Xu Q.‐X. Wang J. Ji C.‐K. Wang Y.‐Z. Bi 《Journal of animal physiology and animal nutrition》2018,102(1):e279-e287
This study was designed to investigate effects of xanthophylls on serum lipid profile (triglyceride, TG; cholesterol, CHO; high‐density lipoprotein cholesterol, HDLC; and low‐density lipoprotein cholesterol, LDLC) and nuclear factor (peroxisome proliferator‐activated receptor gamma, PPARγ; PPAR gamma coactivator 1 alpha, PGC1α; retinoid X receptor gamma, RXRγ; and retinoic acid receptor alpha, RARα) gene expression of breeding hens and chicks. In experiment 1, 432 hens were divided into three groups and fed diets supplemented with 0 (as control group), 20 or 40 mg/kg xanthophylls. Blood was sampled at 7, 14, 21, 28 and 35 days of trial. Liver, duodenum, jejunum and ileum were sampled at 35 days of trial. Results showed that serum HDLC level of hens was increased after dietary 40 mg/kg xanthophyll addition for 21, 28 and 35 days, while serum TG, CHO and LDLC were not affected. Xanthophyll addition also increased PPARγ expression in jejunum, RXRγ expression in duodenum and jejunum, and RARα expression in liver and duodenum. Experiment 2 was a 2 × 2 factorial design. Male chicks hatched from 0 or 40 mg/kg xanthophyll diet of hens were fed diet containing either 0 or 40 mg/kg xanthophylls. Liver, duodenum, jejunum and ileum were sampled at 0, 7, 14 and 21 days after hatching. Blood samples were also collected at 21 days. Results showed that in ovo xanthophylls elevated PPARγ in duodenum and jejunum, and RXRγ and RARα in liver of chicks mainly within 1 week after hatching, while dietary xanthophylls increased serum HDLC level and PPARγ and RXRγ in liver from 2 weeks onwards. In conclusion, our research suggested xanthophylls can regulate serum lipid profile and nuclear factor expression in hens and chicks. 相似文献
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《中国兽医学报》2017,(10):2014-2019
为探讨miR-23b-3p对3T3-L1前脂肪细胞增殖和成脂分化的影响,通过化学修饰的miR-23b-3p模拟物成功转入3T3-L1前脂肪细胞后,利用CCK-8法和流式细胞术检测过表达miR-23b-3p对3T3-L1前脂肪细胞增殖的影响;并通过实时荧光定量PCR和Western blot检测细胞周期基因CyclinD1和CDK4以及成脂标志基因PPARγ和FABP4的表达情况;油红O染色测定甘油三酯积累情况。结果显示,过表达miR-23b-3p抑制3T3-L1前脂肪细胞增殖,导致细胞G1期阻滞,CyclinD1、CDK4在mRNA和蛋白水平极显著下调;在3T3-L1前脂肪细胞成脂分化过程中,miR-23b-3p的表达水平上调;过表达miR-23b-3p促进3T3-L1前脂肪细胞甘油三酯积累,PPARγ、FABP4在mRNA和蛋白水平显著或极显著上调。结果表明,miR-23b-3p能够抑制3T3-L1前脂肪细胞增殖并促进其分化为成熟脂肪细胞。 相似文献
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