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1.
对引入青海高原的２７只北极狐作２０项生理生化参数测定。测定结果：ＲＢＣ，ＷＢＣ，Ｈｂ，嗜中性白细胞，淋巴细胞，单核细胞，嗜酸性、嗜碱性白细胞分别为９．４０±１．８６（１０￣（１２）／Ｌ），７．４０±１．２８（１０￣９／Ｌ），１４．２０±１．８（ｇ／ｄｌ），４０．５８±１．５５（％），５６．５０±１．９３（％）．２．４０±０．７５（％），０．８１±０．０８（％），０．２９±０．０６（％）；血清总蛋白，Ａ／Ｇ，尿素氮，血清总脂，淀粉酶，血糖，Ｋ￣＋，Ｎａ￣＋，Ｃｌ￣－，Ｃａ￣（２＋），Ｍｇ￣（２＋），Ｐ分别为５８．４１±５．３０（ｇ／Ｌ），１．８０±０．１８，６．６９±１．５５（ｍｍｏｌ／Ｌ），７．８８±０．６６（ｇ／Ｌ），６２１±８７．０７（Ｕ／ｄｌ），５．２２±０．６３（ｍｍｏｌ／Ｌ），２．２４±０．５３（ｍｍｏｌ／Ｌ），１７７．０８±１３．４２（ｍｍｏｌ／Ｌ），１０５．５０±１１．６０（ｍｍｏｌ／Ｌ），２．４８±０．４５（ｍｍｏｌ／Ｌ），１．１７±０．２３（ｍｍｏｌ／Ｌ），２．４１±１．０６（ｍｍｏｌ／Ｌ）；同时测得Ｔ，Ｐ，Ｒ分别为３９．２±０．３９（℃），１２７±１０．３（次／ｍｉｎ），２９．２±５．２ 相似文献
2.
商品肉鸡在西宁地区生理常值的测定 总被引：2，自引：0，他引：2
对明星、ＡＡ肉仔鸡在海拔２２００ｍ处的西宁地区进行生理常值的测定，结果为：１．呼吸频率高于文献记录（Ｐ＜０．０５），体温、心率与之相似；２．明星和ＡＡ鸡红细胞数、血红蛋白及ＰＣＶ分别为２．７３±０．３７和２．９３±０．４４百万／ｍｍ３，１１．３±１．３和１２．６±３．４ｇ／Ｌ，４３．６±３．９和４１．７±４．３ｍｌ％，与文献记载均有差异（Ｐ＜０．０５）；３．两品种的ＭＣＨ、ＭＣＶ分别为３８．８±１４．５和４６．１±８．１ｐｇ，１５９．２±２３．０和１７．１±１３．６Ｕ３，显著高于卢宗藩资料（Ｐ＜０．０５）。明星鸡ＭＣＨＣ低于王文三资料，ＡＡ鸡的高于其数据（Ｐ＜０．０５）；４．白细胞分类计数表明，两品利的嗜酸、嗜碱、异嗜、淋巴和单核细胞均与资料有一定变化。 相似文献
3.
柴达木地区幼年双峰驼14项血液生化指标测定 总被引：1，自引：0，他引：1
对青海省柴达木地区莫河驼场３７峰幼年双峰驼的１４项血液生化指标进行测定。结果：血清总蛋白７２．３３±１０．７ｇ／Ｌ，血清蛋白电泳，Ａ７６．５３±６．６５％，α１２．１８×０．９８，α２３．５４×１．６４，β９．８７×３．４６％，γ７．８８×２．９２％，Ａ／Ｇ３．５７×１．１２％；血清尿素氮８．３５×１．９５ｍｍｏｌ／Ｌ；血清总脂３．７０×０．１３ｇ／Ｌ；血清胆固醇３．２３×１．０３ｍｍｏｌ／Ｌ； 相似文献
4.
含野血牦牛八项血清生化指标测定 总被引：1，自引：0，他引：1
对大通种牛场８０头含野血牦牛８项血清生化指标进行了测定。结果：含野血成年牦牛的血清钾、钠、总钙、游离钙、游离钙百分率、氯、无机磷、总蛋白分别为６．０１±０．９３ｍｍｏｌ／Ｌ，１３３．０５±５．４５ｍｍｏｌ／Ｌ，２．４５±０．４２ｍｍｏｌ／Ｌ，１．０２±０．４７ｍｍｏｌ／Ｌ，３９．３４±１３．４２％，１００．９４±７．３６ｍｍｏｌ／Ｌ，２．５３±０．５４ｍｍｏｌ／Ｌ，６７．４０±７．３０ｇ／Ｌ；２～３月龄含野血牦牛的相应指标分别为４．９２±０．６７ｍｍｏｌ／Ｌ，１３５．７５±４．９９ｍｍｏｌ／Ｌ，２．０３±０．２７ｍｍｏｌ／Ｌ，０．４６±０．２２ｍｍｏｌ／Ｌ，２１．３３±９．０３％，９７．３２±４．２４ｍｍｏｌ／Ｌ，３．０６±０．２２ｍｍｏｌ／Ｌ，７５．１±３．２ｇ／Ｌ。 相似文献
5.
新品系兔（A）主要繁殖生理研究 总被引：1，自引：0，他引：1
通过对新培育的Ａ系公鸡兔生殖器官发育测定，采精，配种，成熟卵泡观察，血清雌二醇，睾丸酮含量测定，排卵数，胚胎成活率测定表明：新品系Ａ兔的性成熟时间公兔－４．５月龄左右，适宜的配种时间公兔５～５．５月龄，体重２．８～３．２千克，母兔４．５～５．０日龄，体重达２．７千克。新品系兔初产排卵数７．８５±１．０个，经产排卵数８．５９±０．９６个，高于德国白兔，早期胚胎成活率９０．２％，全期胚胎成活率８５．７ 相似文献
6.
7.
对青海省乐都县５１头驴的１３项血清（浆）生化参数进行测定，结果：总蛋白为７４．４±９．７ｇ／Ｌ，Ａ／Ｇ为Ｏ．９±０．２，总脂为２．５±０．６ｇ／Ｌ，胆固醇为２．５±０．９ｍｍｏｌ／Ｌ，Ｋ￣＋为４．５±０．５ｍｍｏｌ／Ｌ，Ｎａ￣＋为１３７±３ｍｍｏｌ／Ｌ，Ｃａ￣（２＋）为２．３±０．３ｍｍｏｌ／Ｌ，Ｍｇ￣（２＋）为１．６±０．４ｍｍｏｌ／Ｌ，无机磷为２．０±０．６ｍｍｏｌ／Ｌ，ＧＰＴ为８．４±６．４ＩＵ／Ｌ，ＧＯＴ为１１．６±９．７ＩＵ／Ｌ，血浆葡萄糖为５．６±０．５ｍｍｏｌ／Ｌ；上述参数在骟、母驴间无显著差异。 相似文献
8.
GnRH类似物LRH—A3配合FSH对超排母牛胚胎质量的影响 总被引：5，自引：0，他引：5
对４５头供体母牛随机用ＦＳＨ＋ＬＲＨ－Ａ３和ＦＳＨ进行超排处理。其中ＦＳＨ＋ＬＲＨ－Ａ３处理供体５２头次，ＦＳＨ处理供体２２头次；ＦＳＨ＋ＬＲＨ－Ａ３头处理组平均获得卵数，可用胚胎数分别是１０．１±８．０枚，７．４±６．９枚，与ＦＳＨ处理组（二指标分别是９．３±６．２枚，３．６±２．７）相比，分别高８．６％，１０５．６％，且二组超排获得可用胚胎数差异显著（Ｐ＜０．０５）。 相似文献
9.
选择新西兰母兔１０只，于交配前后及妊娠不同时期采血，测定ＬＨ、雌二醇（Ｅ２）、孕酮（Ｐ）浓度。结果显示：ＬＨ水平在交配前４小时为１８．０２±３．０５ｎｇ／ｍｌ，交配后４小时达４３．１４±８．７５ｎｇ／ｍｌ，交配后８小时达高峰水平１６５．１２±２６．４３ｎｇ／ｍｌ，是交配前９倍，其后逐渐降至交配前水平。Ｅ２浓度在交配前５天时为４２．５±１０．１ｐｇ／ｍｌ，交配前４小时为６８．７±１２．１ｐｇ／ｍｌ，交配后４小时为１１３．１±１３．７ｐｇ／ｍｌ，交配后８小时为１１８．５±１５．４ｐｇ／ｍｌ，其后渐降。孕酮浓度在受孕后逐步升高，交配前４小时至交配后２天，孕酮水平在１．０ｎｇ／ｍｌ左右，交配后第２天时，Ｐ水平为１．１２±０．５３ｎｇ／ｍｌ，而在交配后第３～８天时，Ｐ水平达３．８２±０．７２～１０．１０±２．３４ｎｇ／ｍｌ，是交配前后的３～１０倍，交配后第２１天，Ｐ水平为１８．９４±３．９８ｎｇ／ｍｌ，交配后２９天为４．０２±１．０５ｎｇ／ｍｌ。因此，家兔通过交配刺激排卵，也伴有ＬＨ峰值。交配３天后，Ｐ水平大幅度提高，表明家兔妊娠也需要高水平孕酮，同时也为通过测定Ｐ水平进行家兔早期妊娠诊断提供理论基础 相似文献
10.
番鸭精清中几种化学成分的分析 总被引：2，自引：1，他引：1
本文用离子选择电极法测定番鸭精清中几种无机离了的含量，用氨基酸自动分析仪测定精清中游离氨基酸的含量；此外，还测定了番鸭精清中葡萄糖，果糖等有机物质的含量以及几种酶的活力。结果表明，番鸭精清中Ｎａ＾＋，Ｋ＾＋，Ｃａ＾２＾＋，Ｍｇ＾２＾＋和Ｄｌ＾－的浓度分别为：１２６．２±１０．８，１０．７±２．５８，３．５３±０．７９，３．９６±０．９３和１２４．３±１０．６ｍｍｏｌ／Ｌ；在所有被测定的游离氨基酸中 相似文献
11.
S A Center B H Baldwin H N Erb B C Tennant 《Journal of the American Veterinary Medical Association》1985,187(9):935-940
The clinical usefulness of measuring serum bile acid concentrations as a diagnostic test for hepatobiliary disease, was examined in 150 dogs that were suspected of having hepatic disease. Serum values of total bilirubin (TB), alkaline phosphatase (ALP), alanine transaminase (ALT), and albumin were also measured. Fasting serum bile acid (FSBA) values were determined, using a solid-phase radioimmunoassay for total conjugated bile acids or a direct enzymatic spectrophotometric method. A definitive diagnosis was established by histologic examination of the liver. On the basis of histologic findings, dogs were assigned to groups (1 to 8, respectively) including: extrahepatic bile duct obstruction, cirrhosis, portal systemic vascular anastomosis (PSVA), hepatic necrosis, intrahepatic cholestasis, steroid hepatopathy, neoplasia, and secondary disease. Dogs in group 8 had no morphologic evidence of hepatobiliary disease or had mild hepatic lesions. Test efficacies of FSBA, TB, ALP, ALT, and albumin were expressed using 4 indices: sensitivity, specificity, and positive-predictive and negative-predictive values. The diagnostic efficacy of FSBA was examined alone and in combinations with the other tests. There was wide overlapping of FSBA values among dogs in groups 1 to 7, and there was wide overlapping of ALT and ALP values among dogs in all groups. The specificity of FSBA for the diagnosis of liver disease exceeded 90% at values greater than or equal to 30 mumol/L and reached 100% at greater than or equal to 50 mumol/L. Individual liver tests with the best sensitivity for each group were:FSBA and ALP for extrahepatic bile duct obstruction; FSBA for cirrhosis and PSVA; ALT for hepatic necrosis; and ALP for intrahepatic cholestasis, steroid hepatopathy, and neoplasia. Combinations of tests with the best sensitivity for each group were: FSBA + ALP for extrahepatic bile duct obstruction; FSBA + ALT for cirrhosis and PSVA; FSBA + ALT and TB + ALT for hepatic necrosis; and FSBA + ALP for intrahepatic cholestasis, steroid hepatopathy, and neoplasia. Individual tests had the best sensitivity.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
12.
Epelu-Opia J Madej A 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1988,17(1):25-28
A rapid method for determination of progesterone in bovine, goat and porcine plasma as well as in bovine milk was evaluated. The method employed was a solid-phase (125)I radioimmunoassay equipped with progesterone standards in human serum and called the Coat-A-Count procedure. The dilution curves of bovine plasma samples with high progesterone content were parallel with the standard curve based on human serum. The relation between measurements of progesterone levels in bovine plasma using the reference extraction method and the direct Coat-A-Count procedure was highly significant, resulting in the linear regression equation Y = 1.06x-0.04. In case of goat and porcine plasma, the direct method yielded higher results than the reference extraction method (Y = 1.37x + 1.38 and Y = 1.69x - 6.47, respectively). Progesterone concentration in bovine whole milk was much higher when measured by the Coat-A-Count procedure than by the reference Farmos kit (Y = 1.59x + 1.51). However, when the same samples were assayed by a modified Coat-A- Count procedure, i.e. progesterone standards from Farmos kit, the values were more or less identical (Y = 0.88x - 0.21). 相似文献
13.
J Ruprich 《Veterinární medicína》1983,28(6):361-366
The method of preparing and identifying small quantities of aflatoxin B2a after Pohland et al. (1968) was verified as it was necessary to differentiate various types of aflatoxins in analytic practice. Aflatoxin B2a was prepared by the hydroxylation of aflatoxin B1 in acid medium and was identified by thin silica gel layer chromatography (TLC) and by spectral analysis in methanol medium and in a mixture of methanol with ammonium hydroxide (14 + 1). The results of both methods of identification were in keeping with the results of earlier works. At TLC in the system of chloroform + methanol (95 + 5) the Rf values were as follows: Rf AFB1--0.68 and Rf AFB2a--0.32; in the system of chloroform + acetone + isopropyl alcohol (85 + 10 + 5) the respective values were Rf AFB1--0.46 and Rf AFB2a--0.22. Spectral analysis demonstrated a shift in the absorption maximum in the methanol + ammonium hydroxide medium from 362 nm to 404 nm in aflatoxin B2a. The yield rate of AFB2a was about 50 to 55%. 相似文献
14.
N Ek 《Acta veterinaria Scandinavica》1970,11(2):295-304
A method for the rapid electrophoresis on a cellulose acetate membrane of serum proteins from horses, sheep and pigs is discussed.The various main globulin fractions in the serum of these animals were experimentally identified.Normal values for the percentage composition of serum from normal horses, sheep and pigs were calculated.In the horse there was great individual variation in the shape of the β-fraction, assumed to be due to different transferrin types. The mean value for β-globulin of 19.5 % in the horse was higher than for the other two species.The albumin percentage was highest in the sheep and lowest in the pig, 48.5 % and 43.2 % respectively. The sheep had the highest γ-globulin percentage, 22.8 %, while the horse had the lowest with 19.0 %.Finally the values were compared with corresponding figures reported by other authors and the results discussed. 相似文献
15.
Ziv G 《The Veterinary quarterly》1980,2(4):206-210
Summary The minimal inhibitory concentrations (M1C) of tiamulin and tylosin for mycoplasma. Gram-positive, and Gram-negative micro-organisms isolated from chickens were determinated by the agar dilution method. Median M1C values for tiamulin against Mycoplasma gallisepticum (0.05 μg/ml) and Mycoplasma synoviae (0.10 μg/ml) were 2 to 4 times lower than the corresponding values for tylosin. Tiamulin was also slightly more effective in vitro in inhibiting Escherichia coli, Pasteurella multocida, and beta-haemolytic streptococci than was tylosin. Groups of chicken were offered tiamulin medicated drinking water at rates of 125 and 250 mg/litre for 48 hours. Average serum tiamulin concentrations were 0.38 and 0.78 μg/ml, respectively. When tylosin tartrate was added to the drinking water at 500 and 700 mg/litre, average serum drug levels were 0.12 and 0.17 μg/ml, respectively. Tiamulin was 45% bound in chicken serum, as against 30% serum protein binding or tylosin. Correlations were made between free (non protein bound) serum drug levels and the MIC values of the two drugs. Such comparisons suggest that when tiamulin is given in the drinking water at rates of 125 to 250 mg/litre, better antimycoplasmal activity is to be expected in vivo than by giving tylosin tartrate in the drinking water at 500 to 700 mg/litre. Based on these data, no clinical efficacy of these dose rates can be expected in flocks infected by gram-negative microorganisms such as E. coli or P. multocida. The tylosin tartrate rate of 500 to 700 mg/litre, may be clinical ineffective the treatment of Staphylococcus aureus infections. 相似文献
16.
本研究旨在研究日粮添加水果提取物对羔羊生长和屠宰性能、组织器官抗氧化及肉品质的影响.试验将60只体重接近的羔羊随机分为2组,每组6个重复,每个重复5只.在为期4周的饲养期间,对照组饲喂基础日粮,处理组饲喂基础日粮+150?mg/kg水果提取物.结果:粮添加150?mg/kg水果提取物对羔羊屠宰率、热胴体重、冷胴体重、内... 相似文献
17.
A Shirazi N Shams‐Esfandabadi E Ahmadi B Heidari 《Reproduction in domestic animals》2010,45(3):530-536
The objective of this study was to determine the effect of the presence of recombinant ovine growth hormone either alone or together with follicle stimulating hormone (FSH) during ovine oocyte in vitro maturation (IVM) on nuclear maturation and subsequent embryo development. Moreover, the effect of growth hormine (GH) on embryo development whether influenced by the presence of foetal bovine serum (FBS) was assessed. The abattoir‐derived oocytes were randomly divided into four treatment groups and cultured in maturation medium supplemented with: (i) 0.05 IU/ml FSH; (ii) 300 ng/ml roGH; (iii) FSH + roGH; and (iv) no FSH and GH (control). The percentages of germinal vesicle‐stage oocytes in GH‐treated group after 8 h of culture was significantly higher than the FSH and FSH + GH groups and lower than control (22.4%, 8.7%, 9.1%, and 32% respectively). The percentage of MII‐stage oocytes was significantly increased in the presence of GH after 16 and 24 h of culture compared to the control (44.7% and 83.1% vs 32.6% and 73.6% respectively). There was no significant synergism between GH and FSH in terms of nuclear maturation. The blastocyst rates in serum‐supplemented groups were enhanced by the presence of FSH and GH compared to the control (35.4% and 31.3 vs 11.4% respectively). Compared with either GH or FSH alone, the subsequent embryo development (blastocyst rate), however, was negatively influenced by co‐presence of both hormones (22.8%). In contrast, the corresponding values were not affected in the absence of serum. In conclusion, GH had positive effect on nuclear maturation of sheep oocytes. Moreover, the pattern of the effect of GH on embryo development was influenced by the presence of FBS during IVM. 相似文献
18.
S A Center B H Baldwin H Erb B C Tennant 《Journal of the American Veterinary Medical Association》1986,189(8):891-896
The clinical usefulness of measuring serum bile acid concentrations as a diagnostic test for hepatobiliary disease was examined in 80 cats that were suspected of having hepatic disease. Serum values of total bilirubin, alkaline phosphatase (ALP), alanine transaminase (ALT), and aspartate transaminase (AST) also were measured. Fasting serum bile acid values were determined by use of solid-phase radioimmunoassay for total conjugated bile acids or by a direct enzymatic spectrophotometric method. A definitive diagnosis was established by histologic examination of the liver, and on the basis of these findings, cats were assigned to groups (1 to 8, respectively) including: extrahepatic bile duct obstruction, hepatic lipidosis, cirrhosis, intrahepatic cholestasis (cholangiohepatitis, cholangitis), neoplasia, hepatic necrosis, portosystemic vascular anomalies, and miscellaneous. Cats in group 8 had no morphologic evidence of hepatobiliary disease or had hepatic lesions that were mild. Test efficacy of fasting serum bile acids, total bilirubin, ALP, ALT, and AST were expressed by use of 4 indices: sensitivity, specificity, positive predictive value, and negative predictive value. The diagnostic efficacy of fasting serum bile acids was examined alone and in combinations with the other tests. There was wide overlapping of values of fasting serum bile acids, total bilirubin, ALP, ALT, and AST among cats in groups 1 to 7. The specificity of fasting serum bile acids for the diagnosis of hepatic disease exceeded 90% at values greater than or equal to 5 mumol/L and reached 100% at greater than or equal to 15 mumol/L.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
19.
Method for determining serum pepsinogen concentration, using pepsin standards and ultraviolet absorbance 总被引：1，自引：0，他引：1
A simplification of the traditional hemoglobin methods for determining serum pepsinogen concentration was developed. In this method, 10% trichloroacetic acid solution was added to control samples, and hemoglobin substrate was added to controls and active enzyme samples; standards and samples were incubated for 18 hours, the proteins in the active tubes were precipitated with trichloroacetic acid and removed by filtration, and the absorbances of the supernatant of each standard and sample at 280 nm were measured. The major differences between this method and other methods for determining pepsinogen values are that the preacidification of serum with hydrochloric acid was eliminated, the incubation period was reduced to 18 hours (down from 24 hours), the relative pepsinogen concentration was determined by measuring the concentration of hydrolysis products, using ultraviolet, rather than visible absorbance, and a pepsin standard curve was used to determine the serum pepsinogen concentration. Comparison of freshly prepared pepsinogen and pepsin standard curves indicated that the pepsinogen preparations were slightly more active than the pepsin preparations (on a weight-to-weight basis) on the same substrate. Pepsin standards are used because they are more stable than pepsinogen standards. Three linear standard curve ranges were used: O 10 to 100, 50 to 300, and 100 to 500 ng of pepsinogen/ml of serum. The use of pepsin standard curves permits some variability of the incubation conditions without altering the results. For best results, the hemoglobin substrate solution should be prepared daily. This method may be useful in diagnosing ostertagiasis. 相似文献
20.
Relationship of serum and plasma copper and ceruloplasmin concentrations of cattle and the effects of whole blood sample storage 总被引：2，自引：0，他引：2
The present study was conducted to determine whether plasma and serum copper and ceruloplasmin concentrations in cattle are different and whether transport of samples with storage on ice before centrifugation affects the measurements. Mean copper and ceruloplasmin values were higher in plasma than in serum. Linear regressions were plasma copper (microgram/ml) = 1.200 serum copper -0.032 (r2 = 0.99), serum ceruloplasmin (mg/dl) = 14.0 serum copper + 2.34 (r2 = 0.48), and plasma ceruloplasmin (mg/dl) = 18.2 plasma copper + 2.1 (r2 = 0.43). The percentage of copper associated with ceruloplasmin was less in serum (55%) than in plasma (66%). Storage of blood samples on ice for 3 days decreased serum copper value by 3.5%. Linear regressions to correct for storage effects were corrected serum copper = 1.11 stored serum copper -0.04 (r2 = 0.94) and corrected plasma copper = 1.22 stored plasma copper -0.17 (r2 = 0.86). A cuproprotein may be involved in the blood clotting process, and some ceruloplasmin and its copper are apparently trapped in the fibrin clot, causing less copper in serum, compared with that in plasma. The difference between plasma and serum copper concentrations of calves was slightly increased by dietary copper supplementation. 相似文献