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以常用地被植物孔雀草1 a生植株为试材,研究了其浸提液对多年生黑麦草种子萌发的影响,初步了解孔雀草对多年生黑麦草种子萌发的化感作用.结果表明:种子萌发过程中表现情况以及发芽率、发芽势等显示,孔雀草不同部位浸提液对多年生黑麦草种子萌发均具有明显的化感作用,25％的孔雀草根与茎水提液对多年生黑麦草种子萌发具有明显的促进作用,以根水提液促进效果好；其它处理的水提液对多年生黑麦草种子的萌发均起到一定的抑制作用,抑制强度为花水提液＞茎水提液＞叶水提液＞根水提液,初步认定孔雀草存在化感作用潜势. 相似文献
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从生长120 d的辣椒植株上采集叶子,经风干后制成20 g/L、40 g,L和80g/L浓度的水浸提液,处理2叶1心的辣椒根系土壤,30 d后测定辣椒根区土壤的细菌、真菌和放线菌的群落数量以及土壤脲酶和土壤过氧化氢酶活性.结果表明,辣椒叶浸提液处理后的辣椒根区土壤的脲酶活性与过氧化氢酶活性之间相关性不明显:土壤细菌与真菌数量呈正相关、与放线菌数量呈负相关,土壤真菌数量与放线菌数量呈负相关;土壤脲酶活性与土壤细菌数量、真菌数量呈正相关,与土壤放线菌数量呈负相关.土壤过氧化氢酶活性与土壤细菌数量、真菌数量呈负相关、与土壤放线菌数量呈正相关.在辣椒叶浸提液作用下,土壤中细菌、真菌有所增加,而放线菌有所减少,土壤脲酶活性和土壤过氧化氢酶活性都有所增加. 相似文献
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辣椒叶浸提液对土壤微生物数量和土壤酶活性的影响 总被引：1，自引：0，他引：1
从生长120d的辣椒植株上采集叶子,经风干后制成20g/L、40g/L和80g/L浓度的水浸提液,处理2叶1心的辣椒根系土壤,30d后测定辣椒根区土壤的细菌、真菌和放线菌的群落数量以及土壤脲酶和土壤过氧化氢酶活性。结果表明,辣椒叶浸提液处理后的辣椒根区土壤的脲酶活性与过氧化氢酶活性之间相关性不明显:土壤细菌与真菌数量呈正相关、与放线菌数量呈负相关,土壤真菌数量与放线菌数量呈负相关;土壤脲酶活性与土壤细菌数量、真菌数量呈正相关,与土壤放线菌数量呈负相关。土壤过氧化氢酶活性与土壤细菌数量、真菌数量呈负相关、与土壤放线菌数量呈正相关。在辣椒叶浸提液作用下,土壤中细菌、真菌有所增加,而放线菌有所减少,土壤脲酶活性和土壤过氧化氢酶活性都有所增加。 相似文献
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以三角梅植株茎、叶、花为试材,采用最低抑菌浓度试验和测定抑菌圈大小试验,研究茎、叶、花水提取液对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的抑菌效果。结果表明:茎、叶、花水提取物对3种菌均有抑菌效果,通过测定它们的最低抑菌浓度(MIC)及抑菌圈大小可知,三角梅叶水提取液的抑菌效果最好,其次是茎,花的抑菌效果最差。茎、叶水提取液对大肠杆菌的抑菌效果最好,花水提取液对金黄色葡萄球菌的抑菌效果最好。通过不同pH值条件下茎、叶、花水提取液抑菌效果测定,在酸性条件下,水提取液对各菌种的抑菌效果较好。该试验为进一步开发利用三角梅资源,为天然食品防腐保鲜剂的开发提供理论依据。 相似文献
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蚯蚓粪缓解大棚黄瓜连作障碍的研究 总被引：15，自引：3，他引：12
采用室内盆栽的方法,通过向大棚连作土壤中添加蚯蚓粪来解除黄瓜连作障碍。结果表明:蚯蚓粪可以显著改善大棚连作土壤中黄瓜幼苗的生长,提高其根际土壤的肥力,可明显减轻大棚黄瓜土壤的连作障碍问题。蚯蚓粪处理的黄瓜幼苗的株高显著高于对照,其提高率在17.7%~34.4%;而其地上部干重比对照提高了12.5%~26.7%。黄瓜根际土壤可培养微生物的变化亦比较显著。添加蚯蚓粪的处理可培养细菌、真菌、放线菌,其数量随黄瓜幼苗培养时间延长而减少,但是细菌/真菌(B/F)比值呈现增加趋势,表明经过蚯蚓粪处理的黄瓜连作土壤肥力提高。 相似文献
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不同生防菌对黄瓜根际土壤微生物数量及土壤酶活性的影响 总被引：5，自引：0，他引：5
采用盆栽试验的方法,于播种时穴施单一生防菌细菌D、放线菌317和木霉菌,研究黄瓜不同生育时期根际土壤中微生物数量及酶活性的动态变化。结果表明:细菌D和放线菌317的施入利于细菌和放线菌数量的增加,同时有效控制土壤中真菌数量的增加;木霉菌的施入利于土壤中放线菌真菌数量的增加;3个菌株对根际土壤中蔗糖酶、脲酶和过氧化氢酶都有促进的作用,在拉秧期各酶活达到最大值。拉秧期的结果表明,接种细菌D和放线菌317的处理可提高根际土壤中细菌的数量,降低真菌的数量,对放线菌数量的影响不明显;接种木霉处理可明显提高根际土壤中放线菌和真菌的数量,对细菌的数量影响不明显;3个菌株处理细菌与真菌数量比约为对照的1～2倍,放线菌与真菌的数量比约为对照的1～3倍。细菌D、放线菌317和木霉处理均可提高黄瓜根际土壤中过氧化氢酶、蔗糖酶及脲酶的活性。 相似文献
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为实现对入侵植物的有效防治与转化利用,以云南地区常见的紫茎泽兰和牛膝菊2种入侵植物为试材,研究了2种植物的根、茎、叶、花各部位的组织浸泡后获得的水提液对犬齿复活线虫的毒杀活性。结果表明:紫茎泽兰和牛膝菊不同采集部位水提液均存在不同程度的毒杀线虫活性,且与水提液浓度和处理时间呈正相关;紫茎泽兰不同组织部位线虫毒杀活性强弱顺序依次为花叶茎根,牛膝菊为茎叶花根。 相似文献
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以苜蓿为试材,研究了中间锦鸡儿根、茎、叶不同浓度水浸提液(0.5、1.0、5.0、10.0、25.0mg/mL)对苜蓿种子萌发及幼苗生长的化感作用。结果表明:中间锦鸡儿不同器官水浸提液均对苜蓿种子萌发表现出低促高抑的效应。中间锦鸡儿根、茎水浸提液对苜蓿胚根的生长表现为促进作用,且随水浸提液浓度的增大呈先增强后减弱的趋势;中间锦鸡儿根、茎水浸提液对苜蓿胚轴主要表现为抑制作用;中间锦鸡儿叶水浸提液对苜蓿胚根和胚轴的生长均表现出低促高抑的效应;中间锦鸡儿根、叶水浸提液对苜蓿幼苗干重的影响表现为低促高抑,而中间锦鸡儿茎水浸提液则以促进作用为主。总体来看,中间锦鸡儿各器官的水浸提液对苜蓿种子的萌发存在化感作用,建议今后在营造锦鸡儿-苜蓿人工复合系统时需考虑种间化感作用。 相似文献
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The effects of combinations of salinity (no salt, 2000 p.p.m. or 4000 p.p.m. of CaCl2 and NaCl, 1:1) and water table (30 cm, 60 cm or 90 cm from the soil surface), on the vegetative growth and tolerance of ‘Golden Japanese’ plum and ‘Mit Ghamre’ and ‘Balady’ peaches were studied. The plants were grown in lysimeters. The growth of the trunk, total shoot length, the increase in shoot length per cm and the fresh weight of top, root and total plant were reduced with increasing salinity of the irrigation water. The effect was accentuated when the plants were maintained at high water table level. The salinity treatments resulted in the death of 43%, 73% and 76% of the plants in the plum, and the ‘Mit Ghamre’ and ‘Balady’ peaches, respectively, indicating that the plum is more tolerant to salinity than the peach. The plants of the salinity treatments showed various symptoms of salt injury, such as leaf burn, defoliation, shoot die-back and finally death. In the peaches, salt injury started to occur in the first growing-season, whereas the salt injury appeared in the plum in the second growing-season. The symptoms were more pronounced in the 4000 p.p.m. treatment than in the 2000 p.p.m. treatment and were more pronounced at the high water table level. The salinity level was the predominant factor and the effect of the water table on the vegetative growth diminished with increase in the salinity level of the irrigation water. 相似文献
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钾肥在小白菜和萝卜上的施用效果 总被引：6，自引：0，他引：6
采用盆栽试验方法研究了施用钾肥对小白菜、萝卜生长发育、产量、品质及钾素含量的影响。结果表明 ,施用钾肥对小白菜和水萝卜的株高、叶片数无显著的影响 ,但可提高水萝卜块根的产量 ,提高幅度为 31.0 4 %～ 97.93%。施用钾肥可提高小白菜和萝卜的蛋白质和VC含量 ,降低硝酸盐含量 ,增加植株的钾素含量 相似文献
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AIM：To establish a fast, accurate and economical technique for culturing mouse pulmonary arteriolar smooth muscle cells (PASMCs), and to explore the effects of hypoxia on the proliferation and apoptosis of the PASMCs. METHODS：In sterile condition, the pulmonary artery was isolated from the male BALB/c mice by digesting with collagenase I, and the cells were cultured in fetal bovine serum-coated flask. Centrifugal procedure was not used during the cell passage. The cell morphology was observed under an inverted phase-contrast microscope. α-Smooth muscle actin was identified by immunocytochemistry and immunofluorescence. The effects of hypoxia on the proliferation and apoptosis of the PASMCs were detected by CCK-8 assay and TUNEL assay. RESULTS：PASMCs were identified by the methods of immunocytochemistry, immunofluorescence staining and observation of morphology. Unlike the rat PASMCs with typical subcultured peak-vally pattern, the mouse PASMCs showed a lot different without a peak-vally pattern. The cells could be subcultured after 5 d to 7 d and there was 3 to 5 generations depending on the activity of the cells. CCK-8 assay demonstrated that the A values of PASMCs exposed to hypoxia increased after 24 h (P＜0.05) as compared with normoxia. TUNEL result showed that the apoptotic index of the PASMCs in hypoxia decreased after 24 h (P<0.05). CONCLUSION：This technique for obtaining cultured mouse PASMCs is simple, fast, accurate and economical. The digestion time is easy to control. Hypoxia promotes the proliferation and inhibits the apoptosis of PASMCs. 相似文献
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小檗碱和壳聚糖抗蔬菜病原真菌活性测定及复合膜制备 总被引：2，自引：0，他引：2
研究了小檗碱和壳聚糖对几种常见蔬菜病原真菌的抑制活性,以及以小檗碱和壳聚糖为主料制备复合膜的方法,并测定了该膜的药物释放效果。试验表明:低浓度(0.234 mg/mL)小檗碱即可抑制辣椒炭疽病菌(Vermicularia capsici)等5种蔬菜病原真菌的生长。20 mg/mL浓度壳聚糖对番茄灰霉病菌(Botrytis cinerea)的抑制率高达65%,而对其余4种果蔬病原真菌也有一定的抑制作用。为了集成这2种天然化合物的优点,制备了小檗碱-壳聚糖复合膜,该膜具有缓释功能,在模拟外部环境(磷酸缓冲液,pH 6.8)条件下,20 d小檗碱累计释放率接近25%,提示其在果蔬贮藏抗菌中的应用价值。 相似文献
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Fresh onion seeds desiccated to 6.0% seed moisture content (SMC) were stored in various packaging materials under different storage conditions. Seeds packed in aluminum-laminated pouches beside those stored with silica gel at 25 °C maintained satisfactory germinability and vigour after 12 months. Desiccated seeds stored in moisture impervious containers produced more vigourous seedlings. Germination potential of onion seeds increased with reduced SMC besides storage in moisture impervious packets along with desiccants as physiological and biochemical attributes are regulated. Seed viability and vigour decreased with accelerated ageing due to increased lipid peroxidation, decreased activities of several free radical and peroxide scavenging enzymes. Electrical conductance of seed leachates also increased with ageing. Thus, adoption of appropriate storage temperature and moisture control techniques would significantly affect onion seed quality, which was due to minimum accumulation of free peroxide radicals and enhanced activity of free radical scavenging enzymes. 相似文献