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《林业工程学报》2016,(3)
为了优化东北刺人参不定根反应器培养体系,获得较高的人参皂苷含量,以东北刺人参无菌苗诱导得到的不定根为试验材料,研究生物反应器不定根培养过程中,蔗糖浓度、生长素IBA浓度、接种量和通气量对皂苷积累的影响。比较3种培养方式发现,生物反应器培养比振荡培养和固体培养更有利于不定根中皂苷积累。在生物反应器培养过程中,随着蔗糖浓度的增加,皂苷含量明显提高,在蔗糖质量浓度为50 g/L达到最大;IBA质量浓度为3 mg/L时最利于皂苷积累;接种量为5 g/L时不定根中皂苷含量和生产量最多,而接种量超过5 g/L不利于皂苷积累;通气量75 m L/min时,皂苷含量达到最高。因此,利用生物反应器培养不定根来生产皂苷,为资源短缺的东北刺人参产品开发提供了新方法,对今后的东北刺人参产品生产具有重要意义。 相似文献
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通过在1年生、2年生辽育1号杨树幼林内间种玉米、大豆、西瓜、向日葵4种作物,研究了这些作物对杨树幼林生长量的影响。结果表明:间种不同作物对辽育1号杨树幼林生长量的影响差异较大,其中间种西瓜、大豆等矮棵作物优势更为明显,间种玉米、向日葵等高棵作物效果不如间种西瓜、大豆等矮棵作物。建议在辽西地区杨树人工林内多选择对水分需求较多的作物间种,以解决杨树生产中对水分的需求,为其丰产创造条件。 相似文献
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从黄河三角洲自然分布的中国柽柳中选出的2个无性系和从新疆引进的13种柽柳,以中国柽柳为对照,进行无性系遗传测定.通过对4年生3片区域试验林存活率、树高和地径生长量的调查及统计分析,证明东柽1号、2号两个无性系表现最好.在土壤含盐量3.2～4.6 g·kg-1的立地条件下,东柽1号、2号存活率均达88.3%;东柽1号树高、地径生长量分别大于对照17.6%、75.1%,东柽2号树高、地径生长量分别大于对照36.2%、28.4%;同时,观测研究了东柽1号、2号优良无性系的生物学特性.4年生柽柳在3地点的树高遗传力分别为0.90、0.89、0.83,地径遗传力分别为0.92、0.96、0.94.对柽柳树高和地径生长量与地点之间的互作分析得出:树种与地点之间的交互作用不显著,试验中生长表现优良的无性系在3个试验点都有很好的适应性. 相似文献
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目前种衣剂已广泛应用于大田作物，在苗期可防治病虫害，补充植株生长土壤所缺乏的微量元素与养分，对提高作物保苗率及产量作用明显[1][2]。但种衣剂在人参、西洋参上的应用尚未见报导，由于人参、西洋参在出苗期易发生地下病虫害，而且难以控制，出苗保苗率较低，因而种衣剂在西洋参上应用的意义重大。本试验首次用种衣剂包衣西洋参种子，以探求其对西洋参出苗、保苗及苗期生长的影响。1材a与方法G、B、C三种种衣剂分别按照四种不同包衣量在播种前一天对西洋参种子进行包衣，4月20日在左家人参试验基地播种，点播密度4O0粒／mZ，共12处… 相似文献
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本文用生物胶作载体,加1250ppm、2500ppm、5000ppm营养液分别配制成生物胶缓释肥,分别施于洋兰试管苗的生长基质中,用叶面积模型Y=-0.32+0.87X(X=1.d)定期测算叶面积增量,以研究生物胶缓释肥对洋兰生长量的影响.运用模糊正交设计分析表明,在施肥方法、营养液浓度和施加次数3个试验因子中,施肥方式是影响生长的主要因子.与直接施加营养液的传统施肥方法相比,生物胶2号缓释肥能有效地促进洋兰试管苗的生长,其平均叶面积生长量提高2倍以上,且肥效持续时间长,成本低廉,具有较高的实用价值. 相似文献
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采取地膜覆盖技术,在靖宇板石引种西洋参,结果表明,单产为1.26 kg·m-2;总皂甙含量为5.97%,其中,单体皂甙(R b1)含量为1.41%,氨基酸总量为7.165%,质量符合国标Ⅰ级品指标,产品质量高于原产地。在较短无霜期地区可以引种西洋参。 相似文献
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Three new dammarane-type triterpene saponins ginsenosides Rh(18) (1), Rh(19) (3) and Rh(20) (4), along with two new triterpene sapogenins 12β,23(R)-epoxydammara-24-ene-3β,6α,20(S)-triol (2) and dammara-(20E)22,25-diene-3β,6α,12β,24S-tetrol (5) were isolated from the stems and leaves of Panax ginseng C. A. Mey. Their structures were elucidated on the basis of spectral evidence and comparisons with literature data. 相似文献
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Nguyen VT Darbour N Bayet C Doreau A Raad I Phung BH Dumontet C Di Pietro A Dijoux-Franca MG Guilet D 《Fitoterapia》2009,80(1):39-42
Bio-guided fractionation of the roots of Paris polyphylla (Trilliaceae), based on inhibition of P-glycoprotein-mediated daunorubicin efflux in K562/R7 cell line, led to isolation and identification of the three saponins 3-O-Rha(1-->2)[Ara(1-->4)]Glc-pennogenine, gracillin and polyphyllin D, and the two ecdysteroids 20-hydroxyecdysone and pinnatasterone. These compounds were tested for multidrug reversion on P-glycoprotein (ABCB1) with both drug-selected and transfected cell lines, and also on Breast Cancer Resistance Protein (BCRP/ABCG2). By contrast to a weak efficiency on BCRP, the three saponins displayed significant effects as inhibitors of P-glycoprotein-mediated drug efflux. 相似文献
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Bioassay-guided fractionation of the n-BuOH extract of the roots of Clematis argentilucida led to the isolation of two new triterpenoid saponins along with a known one, cussonside B (3). By extensive spectral analysis and chemical evidences, the structures of the two new saponins were determined to be 3β-O-[β-D-ribopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl] hederagenin-11,13-dien-28-oic acid (1) and 3β-O-{β-D-ribopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-xylopyranosyl} oleanolic acid (2), respectively. Saponin 1 is the first example of triterpenoid saponins with two double bonds located at C-11 and C-13 in the aglycone from the genus Clematis. The two new saponins exhibited significant cytotoxicity against human leukemia HL-60 cell lines, human hepatocellular carcinoma Hep-G2 cell lines and human glioblastoma U251MG cell lines with a range of IC(50) values from 2.74 to 25.40μM, while 3 showed inactivity against all of the three cancer cell lines. 相似文献
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不同培养条件对金铁锁毛状根生长的影响 总被引：1，自引：0，他引：1
利用发根农杆菌ATCC15834诱导金铁锁获得毛状根,研究金铁锁毛状根的最优培养条件。结果表明,在5种培养基中,以在1/2MS培养基中皂苷的总产量最大;添加3%蔗糖、水解乳蛋白(LH)及IBA利于毛状根的生长及总皂苷的积累;而添加IAA、NAA、6-BA及蛋白胨抑制其生长。 相似文献
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Three new triterpenoid saponins polygalasaponins LI-LIII (1-3) with two acylation groups in oligosaccharide chain, together with three known saponins were isolated from the roots of Polygala japonica Houtt. (4-6). The neuroprotective effects of these compounds on neuron-like PC12 cells were evaluated in vitro. Compounds 5 and 6 show neuroprotective effects in Aβ(25-35) model at the concentration of 10μM. 相似文献
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文冠果扦插技术研究 总被引：1，自引：0，他引：1
为解决文冠果硬枝扦插过程中的关键性技术问题,使用4种生根粉、3种不同浓度、3种不同处理时间对文冠果插穗进行了生根处理试验,结果表明:不同种类的生根粉对插穗生根率的影响达到了极显著水平,2号和4号生根粉处理的插穗生根率最高,其平均值分别为:73.7%和68.7%。生根粉不同浓度对插穗生根率的影响达到了极显著水平,125mg·L-1、250mg·L-1浓度处理的效果最好,生根率达到90%和82%。4号生根粉不同浓度、不同处理时间对文冠果插穗生根率的影响达到了显著水平,125mg·L-1和250mg·L-1浓度处理效果最好,插穗生根率的平均值达到61.67%和53.33%;6h处理时间对插穗生根效果最好,达到68.67%。4号生根粉,采用125mg·L-1浓度,处理6h对文冠果硬枝扦插的效果最好。 相似文献
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Three new triterpene saponins, named asperosaponin A-C (1-3), together with seven known triterpene saponins (4-10) were isolated from the roots of Dipsacus asper. The structures of compounds 1-3 were elucidated on the basis of detailed spectroscopic analysis and chemical methods. Compounds 1-3, 6-10 had significantly protective effects in PC12 cells against the Aβ(25-35) induced cytotoxicity. All the compounds isolated showed no cytotoxic activity against three human cancer cell lines, A-549, Bel-7402 and BGC-823. 相似文献
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A study of an agrisilviculture system comprising Gmelina arborea and soybean (Glycine max) was conducted in the subhumid region of Central India. Above- and below-ground biomass production and distribution of coarse and fine roots were studied in 4-year-old G. arborea, planted at a spacing of 2 × 2 m, 2 × 3 m, 2 × 4 m and 2 × 5 m. The total biomass varied from 10.89 Mg ha–1 to 3.65 Mg ha–1 depending on the tree density. Among the different tree components, stemwood contributed maximum biomass (54.3–79.4%), followed by branches and leaves. Root distribution pattern showed that most of the coarse roots were distributed in the top 40 cm of soil, whereas fine roots were concentrated in the top 20 cm. Coarse root biomass decreased with an increase in spacing. The spread of roots was asymmetrical in trees planted at 2 × 2 m and 2 × 3 m spacings, while it was symmetrical in trees planted at wide spacings. No significant difference was observed in the fine root biomass in different stands. The root:shoot ratio increased with an increase in spacing. Crop (soybean) growth and productivity varied significantly and it increased with a decrease in tree density. Soybean yield varied between 1.5 Mg ha–1 to 2.1 Mg ha–1. The role of root architecture of G. arborea trees on productivity of crops under agri-silviculture system is discussed. 相似文献