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1.
尤桂春林文忠武竞超姚文郑凯玲 《中国南方果树》2019,(6):25-28
利用扫描电子显微镜观察“尤力克柠檬”“大果柠檬”“漳州本地柠檬”“北京柠檬”“江苏2号柠檬”“印度大果柠檬”“费米耐劳柠檬”“四季柠檬”“台湾甜柠檬”“台湾香水柠檬”等10份种质的花粉,500倍观察花粉粒群体,3000倍观察赤道面和极面,10000倍观察外壁纹饰。结果表明,10份种质的花粉均属于单粒花粉,外观形态分为长球形、近球形和扁球形三类,大小等级为小的花粉,萌发孔沟4或5条,沟长均直达两极,沟沿极轴方向等距分布,外壁纹饰均属于网状雕纹。10份种质的花粉在极轴长、赤道轴长、萌发沟数量、外壁网脊宽及50μm2网眼数等指标上有1个或者1个以上存在显著或极显著性差异,可做为不同种质间鉴定的依据。 相似文献
2.
《中国南方果树》2019,(6)
利用扫描电子显微镜观察"尤力克柠檬""大果柠檬""漳州本地柠檬""北京柠檬""江苏2号柠檬""印度大果柠檬""费米耐劳柠檬""四季柠檬""台湾甜柠檬""台湾香水柠檬"等10份种质的花粉,500倍观察花粉粒群体,3 000倍观察赤道面和极面,10 000倍观察外壁纹饰。结果表明,10份种质的花粉均属于单粒花粉,外观形态分为长球形、近球形和扁球形三类,大小等级为小的花粉,萌发孔沟4或5条,沟长均直达两极,沟沿极轴方向等距分布,外壁纹饰均属于网状雕纹。10份种质的花粉在极轴长、赤道轴长、萌发沟数量、外壁网脊宽及50μm~2网眼数等指标上有1个或者1个以上存在显著或极显著性差异,可做为不同种质间鉴定的依据。 相似文献
3.
《园艺学报》2020,(5)
运用扫描电镜对国产凤仙花组30种植物的花粉形态进行观察研究。结果表明:该组植物的花粉普遍表现为单粒4沟花粉,极面观多为矩圆形,具角萌发沟,表面具网状纹饰;此外,不同种花粉在大小、极面观形状、网脊形态、网眼大小、网眼内颗粒状附属物密度等性状上有明显差异,如极面观多为矩圆形,偶见近圆形、近方形等,花粉表面网状纹饰的网眼有疏密均匀和疏密不均匀两种类型,网脊边缘形状有平滑和波状,网眼内颗粒状附属物不同的密度等。综合以上性状将本组植物的花粉划分为2个类型,8个亚类型。本研究的结果表明花粉形态特征,特别是花粉表面纹饰的网眼是否均匀、网脊形态、网眼大小、网眼内颗粒状附属物的密度等特征可作为凤仙花组组下划分的依据,对其种水平的界定也有一定的帮助。 相似文献
4.
明确黄皮花粉的形态特征及种质间的亲缘关系可为其系统分类鉴定提供孢粉学依据。以福建省11个产地的33份黄皮种质花粉为试材,经固定、清洗、逐级脱水、干燥、喷金镀膜等处理,采用扫描电子显微镜观察其形态结构,分别对花粉群体(放大800倍)、花粉个体(放大3500倍)、花粉赤道面中部的外壁纹饰(放大8000倍)等超微形态特征进行照相和相关指标测定,统计分析相关数据。结果表明,黄皮花粉为近球形或长球形,极轴长17.97~23.76μm,赤道轴长17.11~20.55μm,均有3条萌发沟,辐射对称,分布均等,3条沟在极面不会交汇,有沟界极区,属于赤道三沟类型(NPC),外壁纹饰为网状突起。33份种质花粉间差异主要表现在大小、形状、外壁纹饰和萌发沟性状等方面,花粉形态特征具有一致性和多样性,在遗传距离10处可将这些种质分为3类。 相似文献
5.
在扫描电镜下对5个不同种源的杨山牡丹、8个观赏牡丹品种(包括5个中国中原品种和3个日本品种)和3个牡丹芍药杂交种的花粉超微结构进行观察并对其形态指标进行比较研究。结果表明:不同种源及品种间花粉纹饰差异显著。杨山牡丹以穴状纹饰为主,观赏牡丹品种以网状为主,杂交种以皱波-网状为主。牡丹花粉的极轴长、赤道面宽、极轴长/赤道面宽、萌发沟长、萌发沟宽和穿孔直径均大于牡丹芍药杂交种,但脊宽均小于杂交种。通过比较研究芍药属花粉的超微结构,其花粉形态的差异可以作为资源利用和品种鉴定的初步依据,进一步为牡丹园艺品种的起源、演化以及种类间亲缘关系等的研究提供孢粉学证据。 相似文献
6.
薹菜的花粉形态及其演化和分类的探讨 总被引：1，自引：0，他引：1
利用扫描电镜对4个薹菜的花粉形态进行了系统观察和比较分析。结果表明:在4个薹菜品种中‘南京小叶’薹菜的极轴最长,‘花叶薹菜’的极轴最短;品种之间赤道轴的长短差异不明显。花粉形态较为单一,均呈长球形,萌发沟较长,属于N3P4C5型花粉。4个薹菜品种的花粉壁表面均具有清晰的网状纹饰,但网脊宽窄不一,不同品种间网脊的宽窄及网孔大小、形状等特征存在一定的差异。通过聚类分析表明‘南京小叶’薹菜和‘麻叶黑菜’亲缘关系较近,‘京研薹菜’其次,而‘花叶薹菜’单独聚在一起。花粉特征与表型特征之间存在一定相关性,具有重要的分类学意义。 相似文献
7.
应用扫描电子显微镜对绣球属(Hydrangea)29种植物种子微形态特征进行观察,发现其种子形状分为椭球形和长柱形;种子表面纹饰为网脊形或网状多边形,网脊的形态为波状、深波状或近平直;网脊间常具皱褶状纹饰或近光滑。分析结果表明:传统分类中绣球属的离瓣组(sect.Petalanthae)的种子为椭球形,网脊为波状或深波状;脊间多数为不规则纹饰,少数为规则或孔穴纹饰;绣球组(sect.Hydrangea)的种子均为椭球形,网脊近平直,脊间较光滑;星毛组(sect.Cornidia)和挂苦子组(sect.Heteromallae)的种子均为长柱形,大部分具近平直状的脊,脊间较光滑;冠盖组(sect.Calyptranthe)的种子为长柱形或椭球形,表面具网状多边形纹饰,网眼内具洼点。绣球属种子形态及种皮微形态特征除了在组水平上表现出较强的特异性,在组内种水平上也表现出了一定的分类学价值,在某些组水平上种子微形态性状还具有系统学意义。 相似文献
8.
石蒜属植物花粉形态及分类研究 总被引：18，自引：4，他引：18
利用光学显微镜和扫描电镜对石蒜属(Lycoris Herb. ) 13种植物的花粉形态进行了系统观察和比较分析。结果表明, 石蒜属植物花粉形态均为两侧对称, 赤道面观呈舟形或肾形, 极面观为椭圆形或长椭圆形, 具有单萌发槽或萌发沟, 槽长几乎达两端; 花粉的外壁具网状纹饰, 可划分为粗网状纹饰和细网状纹饰两种类型; 根据扫描电镜清楚观察到在网眼底壁及网脊上是否附有小乳突或小瘤状突起, 又划分为无乳突粗网、乳突粗网和无乳突细网、乳突细网4个亚型。通过花粉形态特征结合其他性状的比较分析,对石蒜属植物的种间分类与系统演化关系进行了探讨。 相似文献
9.
《果树学报》2017,(11)
【目的】明确3个猕猴桃栽培种的花粉形态学特征,了解其遗传变异特性、亲缘关系,为花粉的外观形态与种间分类提供依据。【方法】利用扫描电镜对30个品系的花粉粒进行形态观察。【结果】花粉粒外观为长球形或超长球形,极面观均为三裂圆形,可看到3条内陷萌发沟。美味猕猴桃萌发沟长度18.08~31.48μm、萌发沟脊宽度4.65~11.42μm、极轴长度21.54~36.05μm、赤道轴长度9.60~17.66μm,极轴/赤道轴1.65~3.05、大小244.90~608.52μm2。中华猕猴桃萌发沟长度14.09~24.96μm、萌发沟脊宽度4.09~9.37μm、极轴长度15.60~30.11μm、赤道轴长度8.85~17.87μm、极轴/赤道轴1.26~2.65、大小182.58~443.18μm2。软枣猕猴桃萌发沟长度16.34~30.06μm、萌发沟脊宽度6.11~11.23μm、极轴长度18.66~32.11μm、赤道轴长度8.76~18.42μm、极轴/赤道轴1.33~2.68、大小166.62~547.07μm2。表面纹饰存在3种类型:(1)波纹状,美味猕猴桃、中华猕猴桃和软枣猕猴桃样品均包含,占样品总数的53.33%;(2)疣状,美味猕猴桃、中华猕猴桃和软枣猕猴桃样品均包含,占样品总数的36.67%;(3)颗粒状,均为软枣猕猴桃,占样品总数的10.00%。【结论】猕猴桃花粉外观形态和大小在相同种内和相同来源环境背景下存在一定的保守性;整体来看,美味猕猴桃花粉最大,其次是软枣猕猴桃花粉,显著大于中华猕猴桃花粉;花粉大小、萌发沟长度、萌发沟脊宽度、极轴长度、赤道轴长度,中华猕猴桃均显著小于美味猕猴桃,可作为2者划分的孢粉学特征依据。 相似文献
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11.
AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β-particles emission from 188Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by 188Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [3H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of 188Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from 188 Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G0/G1 arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation. 相似文献
12.
多效唑对猕猴桃离体试管苗生长及内源激素的影响 总被引：18，自引：0，他引：18
多效唑（ＰＰ３３３）处理猕猴桃试管苗，降低了其生长强度；植株体内的ＧＡ３、ＩＡＡ和ＺＴ含量下降，ＡＢＡ的含量上升，乙烯释放率增加；并且能降低外源的ＧＡ３和ＩＡＡ促进生长的作用，而外源的ＧＡ３和ＩＡＡ又能不同程度地逆转多效唑的抑制作用，使植株恢复生长。 相似文献
13.
HU Zhi-cheng ZHU Jia-yuan ZHU Bin GUO Dong CHEN Bin ZHANG Kai HU Kun-hua LI Ming-tao TANG Bing 《园艺学报》2011,27(9):1802-1806
AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two-dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.RESULTS: A two-dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially-expressed proteins in keloid and hypertrophic scar identified from the spots of over 4-fold change, including 16 proteins in both keloid and hypertrophic scar (8 up-regulated and 8 down-regulated), 11 only in keloid (9 up-regulated and 2 down-regulated) and 9 only in hypertrophic scar (4 up-regulated and 5 down-regulated).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars. 相似文献
14.
Duncan Brean W. Boyle Shannon Breininger David R. Schmalzer Paul A. 《Landscape Ecology》1999,14(3):291-309
Historic landcover dynamics in a scrubby flatwoods (Tel-4) and scrub landscape (Happy Creek) on John F. Kennedy Space Center were measured using aerial images from 1943, 1951, 1958, 1969, 1979, and 1989. Landcover categories were mapped, digitized, geometrically registered, and overlaid in ARC/INFO. Both study sites have been influenced by various land use histories, including periods of range management, fire suppression, and fire management. Several analyses were performed to help understand the effects of past land management on the amount and spatial distribution of landcover within the study sites. A chi-squared analysis showed a significant difference between the frequency of landcover occurrence and management period. Markov chain models were used to project observed changes over a 100-year period; these showed current management practices being effective at Tel-4 (restoring historic landscape structure) and much less effective at Happy Creek. Documenting impacts of past management regimes on landcover has provided important insight into current landscape composition and will provide the basis for improving land management on Kennedy Space Center and elsewhere. 相似文献
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AIM: Previous studies performed with XBP-01 in vitro indicated that XBP-01 could inhibit vascular smooth muscle cells from being transformed into foam cell and could eliminate the atherosclerotic plaque in C57BL/6J mouse. This experiment is to investigate its mechanism of eliminating plaques in vitro. METHODS: The cultured porcine artery smooth muscle cells incubated with XBP-01 of 0.1 mg/L for 24 h after preincubated with oxidized low density lipoprotein of 15 mg/L for 72 h in vitro. The samples were analyzed by fluorescence microscope, confocal microscope system and flow cytometry. RESULTS: Apoptosis was triggered by being incubated with oxidized low density lipoprotein and this process was accelerated additionally by being incubated with XBP-01. CONCLUSION: XBP-01 can be effective in eliminating atherosclerotic plaque by accelerating the process in which oxidized low density lipoprotein induced smooth muscle cell apoptosis. 相似文献
16.
Species distribution models (SDMs) often use elevation as a surrogate for temperature or utilise elevation sensitive interpolations
from weather stations. These methods may be unsuitable at the landscape scale, especially where there are sparse weather stations,
dramatic variations in exposure or low elevational ranges. The goal of this study was to determine whether radiation, moisture
or a novel estimate of exposure could improve temperature estimates and SDMs for vegetation on the Illawarra Escarpment, near
Sydney, Australia. Forty temperature sensors were placed on the soil surface of an approximately 12,000 ha study site between
November 2004 and August 2006. Linear regression was used to determine the relationship with environmental factors. Elevation
was correlated more with moderate temperatures (winter maximums, summer minimums, spring and autumn averages) than extreme
temperatures (summer maximums, winter minimums). The correlation (r
2) between temperature and environmental factors was improved by up to 0.38 by incorporating exposure, moisture and radiation
in the regressions. Summer maximums and winter minimums were predominately determined by exposure to the NW and coastal influences
respectively, while exposure to the NE and SW was important during other seasons. These directions correspond with the winds
that are most influential in the study area. The improved temperature estimates were used in Generalised Additive Models for
37 plant species. The deviance explained by most models was increased relative to elevation, especially for moist rainforest
species. It was concluded that improving the accuracy of seasonal temperature estimates could improve our ability to explain
the patchy distribution of many species.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
17.
AIM: To detect the expression of cytoplasmic inhibitor of apoptosis protein 2 (c-IAP2) and growth arrest-specific gene 1 (GAS1) in Hodgkin's lymphoma (HL) and anaplastic large cell lymphoma (ALCL) and to investigate the role of two genes in the pathogenesis of HL and ALCL.METHODS: HE staining, the antibodies CD30, CD15, CD45RO and CD20 were used to screen the cases of HL and ALCL from 288 cases of lymphoma. The clarified HL and ALCL were subjected for immunohistochemical staining by SP and ABC methods to analyze the expression of c-IAP2 and GAS1. RESULTS: ①The positive rate of c-IAP2 in HL was 25/26(96.1%) while that in ALCL was 6/19(31.6%), there presented statistic significance between HL and ALCL groups(P<0.05), meanwhile the positive rate of GAS1 showed statistic significance between HL and ALCL groups(P<0.05). ②Two cases were showed to be a mixed type combined with large tumor cells of HL and relatively smaller tumor cells of ALCL.CONCLUSION: ①The different expression of c-IAP2 and GAS1 in HL and ALCL implied a different mechanism of oncogenesis and the different defects in the pathway of signal transduction of apoptosis in HL and ALCL;②Few cases showed an overlap and a likely transitional state between HL and ALCL; ③The different expressing manner of GAS1 and c-IAP2 in HL and ALCL implied the potential marks for the differential dignosis of two kinds of lymphoma. 相似文献
18.
Effects of patch attributes, barriers, and distance between patches on the distribution of a rock-dwelling rodent (Lagidium viscacia) 总被引：1，自引：0，他引：1
We tested whether size of habitat patches and distance between patches are sufficient to predict the distribution of the mountain
vizcacha Lagidium viscacia a large, rock-dwelling rodent of the Patagonian steppe Argentina, or whether information on other patch and landscape characteristics
also is required. A logistic regression model including the distance between rock crevices and depth of crevices, distance
between a patch and the nearest occupied patch, and whether or not there was a river separating it from the nearest occupied
patch was a better predictor of patch occupancy by mountain vizcachas than was a model based only on patch size and distance
between patches. Our results indicate that a simple metapopulation analysis based on size of habitat patches and distance
between patches may not provide an accurate representation of regional population dynamics if patches vary in habitat quality
independently of patch size and features in the matrix alter connectivity.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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β3-adrenergic receptor is the third subtype of β-adrenergic receptors. The genetic structure and pharmacological property of β3-adrenergic receptor are markedly distinguished from β1-and β2-adrenergic receptor subtypes. Recently studies show that myocardial β3-adrenergic receptor mediates negative inotropic effect through Gi-protein/NO/cGMP pathway, the expression of β3-adrenergic receptor and negative inotropic effect mediated by β3-adrenergic receptor are increased in heart failure. However, because of the low expression of β3-adrenergic receptor in the heart, the actual pathophysiological significance of β3-adrenergic receptor remains unknown. 相似文献